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Wound healing by electricity

Anil Sebastian, Susan W Volk, Poonam Halai, James Colthurst, Ralf Paus, Ardeshir Bayat
Electrical stimulation (ES) is known to promote cutaneous healing, however its ability to regulate reinnervation remains unclear. Firstly, we show that ES treatment of human acute cutaneous wounds (n=40) increased reinnervation. Next, to define neurophysiological mechanisms through which ES impacts repair, microarray of wound biopsies was performed on days 3, 7, 10 and 14 post-wounding. This identified neural differentiation biomarkers Class III β-tubulin (TUBB3; melanocyte development and neuronal marker) and its upstream molecule Factor Induced Gene 4 (FIG4; phosphatidylinositol (3,5)-bisphosphate 5-phosphatase) as significantly up-regulated post-ES treatment...
November 14, 2016: Journal of Investigative Dermatology
Rumiana Tzoneva, Veselina Uzunova, Sonia Apostolova, Anne Krüger-Genge, Axel T Neffe, Friedrich Jung, Andreas Lendlein
Angiogenesis is one of the key processes during development, wound healing and tumor formation. Prerequisite for its existence is the presence of endogenous electrical fields (EFs) generated by active ion transport across polarized epithelia and endothelia, and appearance of the transcellular potentials. During angiogenesis cellular factor as endothelial growth factor (VEGF), synthesis of adhesive proteins and membrane metalloproteinases (MMPs) govern the angiogenic response to different external stimuli as biomaterials interactions and/or exogenous EF...
October 27, 2016: Clinical Hemorheology and Microcirculation
Alexander G Volkov, Eunice K Nyasani, Clayton Tuckett, Jessenia M Scott, Mariah M Z Jackson, Esther A Greeman, Ariane S Greenidge, Devin O Cohen, Maia I Volkova, Yuri B Shtessel
Electrostimulation of plants can induce plant movements, activation of ion channels, ion transport, gene expression, enzymatic systems activation, electrical signaling, plant-cell damage, enhanced wound healing, and influence plant growth. Here we found that electrical networks in plant tissues have electrical differentiators. The amplitude of electrical responses decreases along a leaf and increases by decreasing the distance between polarizing Pt-electrodes. Intercellular Ag/AgCl electrodes inserted in a leaf and extracellular Ag/AgCl electrodes attached to the leaf surface were used to detect the electrotonic potential propagation along a leaf of Aloe vera...
October 12, 2016: Bioelectrochemistry
Kehua Zhou, Ronald Schenk, Michael S Brogan
BACKGROUND: Electric stimulation (E-stim) has been found to be an effective treatment in improving wound healing rates. However, the wound healing trajectory and its related predictors for complete wound closure (CWC) have not been reported with E-stim treatment. MATERIALS AND METHODS: This was a retrospective study. Data on 159 patients treated at an outpatient wound clinic utilizing combined intervention of E-stim and conventional care were included. The Kaplan-Meier healing curve together with linear regression models depicted the percentage of patients with CWC against time...
December 2016: European Journal of Clinical Investigation
Youngmi Koo, Yeoheung Yun
Polydeoxyribonucleotides (PDRN) have been explored as an effective treatment for tissue repair in peripheral artery occlusive disease, diabetic foot ulcers, and eye lotion. We report on the effect of polydeoxyribonucleotides (PDRN) on wound healing by using the electric cell-substrate impedance sensing (ECIS) system and viability testing. Human osteoblasts (U2OS) and primary human dermal fibroblasts (HDF) were used to study the effect of PDRN on migration and proliferation. ECIS allowed the creation of a wound by applying high current, and then monitoring the healing process by measuring impedance in real time...
December 1, 2016: Materials Science & Engineering. C, Materials for Biological Applications
Mohammed Ashrafi, Teresa Alonso-Rasgado, Mohamed Baguneid, Ardeshir Bayat
Current gold standard lower extremity cutaneous wound management is not always effective. Cutaneous wounds generate a "current of injury" which is directly involved in wound healing processes. Application of exogenous electrical stimulation has been hypothesised to imitate the natural electric current that occurs in cutaneous wounds. The aim of this extensive review is to provide a detailed update on the variety of electrical stimulation modalities used in the management of lower extremity wounds. Several different waveforms and delivery methods of electrical stimulation have been used...
August 30, 2016: Experimental Dermatology
Spela Kos, Tanja Blagus, Maja Cemazar, Ursa Lampreht Tratar, Monika Stimac, Lara Prosen, Tanja Dolinsek, Urska Kamensek, Simona Kranjc, Lars Steinstraesser, Gaëlle Vandermeulen, Véronique Préat, Gregor Sersa
Skin is an attractive target for gene electrotransfer. It consists of different cell types that can be transfected, leading to various responses to gene electrotransfer. We demonstrate that these responses could be controlled by selecting the appropriate electrotransfer parameters. Specifically, the application of low or high electric pulses, applied by multi-electrode array, provided the possibility to control the depth of the transfection in the skin, the duration and the level of gene expression, as well as the local or systemic distribution of the transgene...
2016: Molecular Therapy. Nucleic Acids
Roger Esteban-Vives, Myung S Choi, Matthew T Young, Patrick Over, Jenny Ziembicki, Alain Corcos, Jörg C Gerlach
Partial and deep partial-thickness burn wounds present a difficult diagnosis and prognosis that makes the planning for a conservative treatment versus mesh grafting problematic. A non-invasive treatment strategy avoiding mesh grafting is often chosen by practitioners based on their clinical and empirical evidence. However, a delayed re-epithelialization after conservative treatment may extend the patient's hospitalization period, increase the risk of infection, and lead to poor functional and aesthetic outcome...
November 2016: Burns: Journal of the International Society for Burn Injuries
William J Ennis, Claudia Lee, Karen Gellada, Thomas F Corbiere, Timothy J Koh
BACKGROUND: Cellular energy is required for the healing cascade to occur. A combination of cells, cytokines, chemokines, tissue perfusion, an extracellular matrix, and local forces are also required to allow for human tissue repair to proceed. Although there are many examples of treatment options, energy-based therapies are the least understood, appreciated, and employed by practicing wound care physicians. The recent growth of tissue engineering has encouraged researchers to employ both electrical stimulation and therapeutic ultrasound (US) to stimulate cells, induce migration, and modify tissue constructs...
September 2016: Plastic and Reconstructive Surgery
Andrew L Frelinger, Anja J Gerrits, Allen L Garner, Andrew S Torres, Antonio Caiafa, Christine A Morton, Michelle A Berny-Lang, Sabrina L Carmichael, V Bogdan Neculaes, Alan D Michelson
BACKGROUND: Activated autologous platelet-rich plasma (PRP) used in therapeutic wound healing applications is poorly characterized and standardized. Using pulsed electric fields (PEF) to activate platelets may reduce variability and eliminate complications associated with the use of bovine thrombin. We previously reported that exposing PRP to sub-microsecond duration, high electric field (SMHEF) pulses generates a greater number of platelet-derived microparticles, increased expression of prothrombotic platelet surfaces, and differential release of growth factors compared to thrombin...
2016: PloS One
Eun Ho Kim, Hyo Sook Song, Seung Hoon Yoo, Myonggeun Yoon
Treatment with alternating electric fields at an intermediate frequency (100-300 kHz), referred to as tumor treating fields (TTF) therapy, inhibits cancer cell proliferation. In the present study, we demonstrated that TTF application suppressed the metastatic potential of U87 and U373 glioblastoma cell lines via the NF-kB, MAPK and PI3K/AKT signaling pathways. Wound-healing and transwell assays showed that TTF suppressed cell migration and invasion compared with controls. Soft agar and three-dimensional culture assays showed that TTF inhibited both anchorage-dependent (cell proliferation) and anchorage-independent (colony formation) GBM cell growth...
August 18, 2016: Oncotarget
Jimmy Toussaint, Won Taek Chung, Steve Mc Clain, Vivek Raut, Adam J Singer
Many deep partial thickness burns require more than 3 weeks to heal resulting in disfiguring and dysfunctional scarring. Early excision of the eschar has been shown to improve outcomes in deep burns; however, the optimal timing of the excision remains controversial. We compared wound healing and scarring of deep partial thickness burns that were excised at different time points in a porcine model. Deep partial thickness burns (2.5 by 2.5 cm each) were created on the backs of six anesthetized pigs using a previously validated model...
July 13, 2016: Journal of Burn Care & Research: Official Publication of the American Burn Association
Qin Qin, Zheng-Ai Xiong, Ying Liu, Chen-Guo Yao, Wei Zhou, Yuan-Yuan Hua, Zhi-Liang Wang
The effects of irreversible electroporation (IRE) on the proliferation, migration, invasion and adhesion of human cervical cancer cell lines HeLa and SiHa were investigated in the present study. HeLa and SiHa cells were divided into a treatment group and control group. The treatment group cells were exposed to electric pulses at 16 pulses, 1 Hz frequency for 100 µsec with 1,000 V/cm strength. Cellular proliferation was determined 24 h after treatment using a Cell Counting Kit‑8 (CCK‑8) assay and carboxyfluorescein diacetate‑succinimidyl ester (CFDA‑SE) labeling assay...
September 2016: Molecular Medicine Reports
Liang Liu, Julie Moody, Angela Gall
Pressure ulcers (PrUs) are among the most common secondary complications following spinal cord injury (SCI). External electrical current applied to a wound is believed to mimic the body's natural bioelectricity and to restart and stimulate endogenous electrical fields to promote wound healing. A systematic review was conducted to critically appraise and synthesize updated evidence on the impact of electrical stimulation (ES) versus standard wound care (comprising cleansing, dressing, nutrition, and debridement as necessary) and/or sham stimulation on PrU healing rates in persons with SCIs...
July 2016: Ostomy/wound Management
Christina L Ross, Ishaq Syed, Thomas L Smith, Benjamin S Harrison
Traumatic spinal cord injury (SCI) is typically the result of direct mechanical impact to the spine, leading to fracture and/or dislocation of the vertebrae along with damage to the surrounding soft tissues. Injury to the spinal cord results in disruption of axonal transmission of signals. This primary trauma causes secondary injuries that produce immunological responses such as neuroinflammation, which perpetuates neurodegeneration and cytotoxicity within the injured spinal cord. To date there is no FDA-approved pharmacological agent to prevent the development of secondary SCI and induce regenerative processes aimed at healing the spinal cord and restoring neurological function...
July 11, 2016: Electromagnetic Biology and Medicine
Hakan Solmaz, Sergulen Dervisoglu, Murat Gulsoy, Yekta Ulgen
Laser biostimulation in medicine has become widespread supporting the idea of therapeutic effects of photobiomodulation in biological tissues. The aim of this study was to investigate the biostimulation effect of laser irradiation on healing of cutaneous skin wounds, in vivo, by means of bioimpedance measurements and histological examinations. Cutaneous skin wounds on rats were subjected to 635 nm diode laser irradiations at two energy densities of 1 and 3 J/cm(2) separately. Changes in the electrical properties of the wound sites were examined with multi-frequency electrical impedance measurements performed on the 3rd, 7th, 10th, and 14th days following the wounding...
November 2016: Lasers in Medical Science
Brandon M Franklin, Eleni Maroudas, Jeffrey L Osborn
Soft tissue repair is a complex process that requires specific communication between multiple cell types to orchestrate effective restoration of physiological functions. Macrophages play a critical role in this wound healing process beginning at the onset of tissue injury. Understanding the signaling mechanisms involved in macrophage recruitment to the wound site is an essential step for developing more effective clinical therapies. Macrophages are known to respond to electrical fields, but the underlying cellular mechanisms mediating this response is unknown...
June 2016: Physiological Reports
Ki-Taek Lim, Hoon Seonwoo, Kyung Soon Choi, Hexiu Jin, Kyung-Je Jang, Jangho Kim, Jin-Woo Kim, Soo Young Kim, Pill-Hoon Choung, Jong Hoon Chung
Electromagnetic fields (EMFs) can modulate cell proliferation, DNA replication, wound healing, cytokine expression, and the differentiation of mesenchymal stem cells (MSCs). Graphene, a 2D crystal of sp(2) -hybridized carbon atoms, has entered the spotlight in cell and tissue engineering research. However, a combination of graphene and EMFs has never been applied in tissue engineering. This study combines reduced graphene oxide (RGO) and pulsed EMFs (PEMFs) on the osteogenesis and neurogenesis of MSCs. First, the chemical properties of RGO are measured...
August 2016: Advanced Healthcare Materials
Ana Delia Pinzón-García, Puebla Cassini-Vieira, Cyntia Cabral Ribeiro, Carlos Eduardo de Matos Jensen, Luciola Silva Barcelos, Maria Esperanza Cortes, Ruben Dario Sinisterra
The present work demonstrated an efficient cutaneous wound healing using Bixin-loaded polycaprolactone (PCL) nanofibers as a controlled delivery system. The influence of Bixin (Bix) content on PCL nanofiber, Bix-PCL1(2.5% w/w bix) and Bix-PCL2 (12.5% w/w bix) formation was investigated using electrical conductivity, attenuated total reflectance infrared spectroscopy, X-ray diffraction, thermal analysis, and scanning electronic microscopy. The results showed that a greater bixin concentration resulted in higher polymeric solution electrical conductivity...
June 13, 2016: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Sandra L Orellana, Annesi Giacaman, Francisca Pavicic, Alejandra Vidal, Ignacio Moreno-Villoslada, Miguel Concha
The study of biomaterials by electrical charge scaling to explore the role of net charge on biocompatibility and suitability for tissue regeneration has been limited as has the search for products that could improve this first-rate variable. In the present study, we prepared sponges composed of chitosan/alginate (CS/ALG) with or without hyaluronic acid (HA) by mixing polymer stock solutions of different net electric charge ratios (n(+/) n(-) ), and then lyophilizing them to obtain porous materials. The electric charge ratios n(+/) n(-) studied were 0...
October 2016: Journal of Biomedical Materials Research. Part A
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