Di Cui, Andrea Mesaros, Gregor Burdeos, Ingo Voigt, Patrick Giavalisco, Yvonne Hinze, Martin Purrio, Bernd Neumaier, Alexander Drzezga, Yayoi Obata, Heike Endepols, Xiangru Xu
Dnmt3a2, a de novo DNA methyltransferase, is induced by neuronal activity and participates in long-term memory formation with the increased expression of synaptic plasticity genes. We wanted to determine if Dnmt3a2 with its partner Dnmt3L may influence motor behavior via the dopaminergic system. To this end, we generated a mouse line, Dnmt3a2/3L Dat / wt , with dopamine transporter (DAT) promotor driven Dnmt3a2/3L overexpression. The mice were studied with behavioral paradigms (e.g., cylinder test, open field, and treadmill), brain slice patch clamp recordings, ex vivo metabolite analysis, and in vivo positron emission tomography (PET) using the dopaminergic tracer 6-[18 F]FMT...
August 31, 2020: International Journal of Molecular Sciences