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https://www.readbyqxmd.com/read/29345463/-%C3%A2-sativamides-a-and-b-two-pairs-of-racemic-nor-lignanamide-enantiomers-from-the-fruits-of-cannabis-sativa-hemp-seed
#1
Guo-Yuan Zhu, Ji Yang, Xiao-Jun Yao, Xing Yang, Jing Fu, Xin Liu, Li-Ping Bai, Liang Liu, Zhi-Hong Jiang
(±)-Sativamides A (1) and B (2), two pairs of nor-lignanamide enantiomers featuring a unique benzo-angular triquinane skeleton, were isolated from the fruits of Cannabis sativa (hemp seed). Their structures were elucidated by detailed spectroscopic analysis and ECD calculations. The resolution of (+)- and (-)-sativamides A and B were achieved by chiral HPLC. Pretreatment of neuroblastoma cells with 1 and 2 significantly reduced the endoplasmic reticulum (ER) stress-induced cytotoxicity.
January 18, 2018: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/29339280/simultaneous-degumming-and-production-of-a-natural-gum-from-crotalaria-juncea-seeds-physicochemical-and-rheological-characterization
#2
Suvra Sadhukhan, Annesha Bhattacharjee, Ujjaini Sarkar, Pabitra Kumar Baidya, Sibashish Baksi
The oil extracted from Crotalaria juncea (Sunn-hemp) contains 70% of gum. Several methods of degumming are attempted in order to maximize the yield of gum. During appropriate water induced degumming, about 95-98% of phosphatides are separated. The maximum oil yield for two types of degummimg processes are 0.59% and 0.69% corresponding to hot water and pure O-phosphoric acid (19.88 N) treatment respectively. The % oil yield obtained for TOP degumming is about 0.78%. Physico-chemical characteristics of the isolated gum such as moisture, ash, protein, fat and aqueous solubility along with FTIR and TGA analysis are studied in order to evaluate the effect of extraction process...
January 12, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29306345/evaluation-of-the-effects-of-different-diets-on-microbiome-diversity-and-fatty-acid-composition-of-rumen-liquor-in-dairy-goat
#3
P Cremonesi, G Conte, M Severgnini, F Turri, A Monni, E Capra, L Rapetti, S Colombini, S Chessa, G Battelli, S P Alves, M Mele, B Castiglioni
Fat supplementation plays an important role in defining milk fatty acids (FA) composition of ruminant products. The use of sources rich in linoleic and α-linolenic acid favors the accumulation of conjugated linoleic acids isomers, increasing the healthy properties of milk. Ruminal microbiota plays a pivotal role in defining milk FA composition, and its profile is affected by diet composition. The aim of this study was to investigate the responses of rumen FA production and microbial structure to hemp or linseed supplementation in diets of dairy goats...
January 8, 2018: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/29204056/effects-of-hemp-seed-soft-capsule-on-colonic-ion-transport-in-rats
#4
Xiao-Fang Lu, Meng-Di Jia, Sheng-Sheng Zhang, Lu-Qing Zhao
AIM: To investigate the effect of Hemp seed soft capsule (HSCC) on colonic ion transport and its related mechanisms in constipation rats. METHODS: Sprague-Dawley male rats were randomly divided into three groups: normal group, constipation group and HSSC group. Rats in the constipation and HSSC groups were administrated loperamide 3 mg/kg per day orally for 12 d to induce the constipation model. Then, the HSSC group was given HSSC 0.126 g/kg per day by gavage for 7 d...
November 14, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/29182999/analysis-of-cannabinoids-in-commercial-hemp-seed-oil-and-decarboxylation-kinetics-studies-of-cannabidiolic-acid-cbda
#5
Cinzia Citti, Barbara Pacchetti, Maria Angela Vandelli, Flavio Forni, Giuseppe Cannazza
Hemp seed oil from Cannabis sativa L. is a very rich natural source of important nutrients, not only polyunsaturated fatty acids and proteins, but also terpenes and cannabinoids, which contribute to the overall beneficial effects of the oil. Hence, it is important to have an analytical method for the determination of these components in commercial samples. At the same time, it is also important to assess the safety of the product in terms of amount of any psychoactive cannabinoid present therein. This work presents the development and validation of a highly sensitive, selective and rapid HPLC-UV method for the qualitative and quantitative determination of the main cannabinoids, namely cannabidiolic acid (CBDA), tetrahydrocannabinolic acid (THCA), cannabidiol (CBD), tetrahydrocannabinol (THC), cannabinol (CBN), cannabigerol (CBG) and cannabidivarin (CBDV), present in 13 commercial hemp seed oils...
February 5, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29112398/new-ace-inhibitory-peptides-from-hemp-seed-cannabis-sativa-l-proteins
#6
Lara P Orio, Giovanna Boschin, Teresa Recca, Carlo F Morelli, Laura Ragona, Pierangelo Francescato, Anna Arnoldi, Giovanna Speranza
A hemp seed protein isolate, prepared from defatted hemp seed meals by alkaline solubilization/acid precipitation, was subjected to extensive chemical hydrolysis under acid conditions (6 M HCl). The resulting hydrolysate was fractionated by semipreparative RP-HPLC, and the purified fractions were tested as inhibitors of angiotensin converting enzyme (ACE). Mono- and bidimensional NMR experiments and LC-MS analyses led to the identification of four potentially bioactive peptides, i.e. GVLY, IEE, LGV, and RVR...
December 6, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29098190/cannabis-sativa-hemp-seeds-%C3%AE-9-tetrahydrocannabinol-and-potential-overdose
#7
Yi Yang, Melissa M Lewis, Angelica M Bello, Ewa Wasilewski, Hance A Clarke, Lakshmi P Kotra
Introduction:Cannabis sativa (hemp) seeds are popular for their high nutrient content, and strict regulations are in place to limit the amount of potentially harmful phytocannabinoids, especially Δ9-tetrahydrocannabinol (Δ9-THC). In Canada, this limit is 10 μg of Δ9-THC per gram of hemp seeds (10 ppm), and other jurisdictions in the world follow similar guidelines. Materials and Methods: We investigated three different brands of consumer-grade hemp seeds using four different procedures to extract phytocannabinoids, and quantified total Δ9-THC and cannabidiol (CBD)...
2017: Cannabis and Cannabinoid Research
https://www.readbyqxmd.com/read/29096438/exploration-of-potentially-bioactive-peptides-generated-from-the-enzymatic-hydrolysis-of-hempseed-proteins
#8
Gilda Aiello, Carmen Lammi, Giovanna Boschin, Chiara Zanoni, Anna Arnoldi
The seed of industrial hemp is an underexploited protein source. In view of a possible use in functional foods, a hempseed protein concentrate was hydrolyzed with pepsin, trypsin, pancreatin, or a mixture of these enzymes. A detailed peptidomic analysis using data-dependent acquisition showed that the numbers of peptides identified ranged from 90 belonging to 33 parent proteins in the peptic hydrolysate to 9 belonging to 6 proteins in the pancreatin digest. The peptic and tryptic hydrolysates resulted to be the most efficient inhibitors of 3-hydroxymethyl-coenzyme A reductase activity, when tested on the catalytic domain of the enzyme...
November 2, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29080417/identification-of-a-sustainable-two-plant-diet-that-effectively-prevents-age-related-metabolic-syndrome-and-extends-lifespan-in-aged-mice
#9
Xiang-Yong Li, Ying-Hua Liu, Bin Wang, Chih-Yu Chen, Hong-Man Zhang, Jing X Kang
The current system of food production is linked to both the increasing prevalence of chronic disease and the deterioration of the environment, and thereby calls for novel ways of producing nutritious foods in a sustainable manner. In the "longevity village" of Bama, China, we have identified two plant foods, hemp seed and bitter vegetable (Sonchus oleraceus), that are commonly consumed by its residents and grow abundantly in unfarmed land without fertilizers or pesticides. Here, we show that a diet composed of these two foods (the "HB diet") provides a sufficient variety of nutrients and confers significant health benefits...
September 19, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/28886910/challenges-towards-revitalizing-hemp-a-multifaceted-crop
#10
REVIEW
Craig Schluttenhofer, Ling Yuan
Hemp has been an important crop throughout human history for food, fiber, and medicine. Despite significant progress made by the international research community, the basic biology of hemp plants remains insufficiently understood. Clear objectives are needed to guide future research. As a semi-domesticated plant, hemp has many desirable traits that require improvement, including eliminating seed shattering, enhancing the quantity and quality of stem fiber, and increasing the accumulation of phytocannabinoids...
November 2017: Trends in Plant Science
https://www.readbyqxmd.com/read/28448475/formulation-characterization-and-properties-of-hemp-seed-oil-and-its-emulsions
#11
Veronika Mikulcová, Věra Kašpárková, Petr Humpolíček, Leona Buňková
The formulation, characterization, and anticipated antibacterial properties of hemp seed oil and its emulsions were investigated. The oil obtained from the seeds of Cannabis sativa L. in refined and unrefined form was characterized using iodine, saponification, acid values, and gas chromatography, and was employed for the preparation of stable oil-in-water emulsions. The emulsions were prepared using pairs of non-ionic surfactants (Tween, Span). The effects of the emulsification method (spontaneous emulsification vs...
April 27, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28444789/effect-of-dietary-supplementation-of-hemp-seed-cannabis-sativa-l-on-meat-quality-and-egg-fatty-acid-composition-of-japanese-quail-coturnix-coturnix-japonica
#12
H Yalcin, Y Konca, F Durmuscelebi
This study was conducted in two separate experiments. Experiment I was conducted to investigate the effects of hemp seed (HS) on meat quality traits, and experiment II was designed to determine egg fatty acid (FA) composition in Japanese quail (Coturnix coturnix japonica). In experiment I and experiment II treatments, groups were as follows: (i) control (not included HS0), (ii) 5% HS inclusion to diets, (iii) 10% HS inclusion to diets and (iv) 20% HS inclusion to diets. In experiment I, a total of 192 7-day-old-quail were fed with HS diets for 5 weeks, and end of the experiment I, a total of 64 quail (16 each) slaughtered and meat samples were analysed for meat colour, pH, cooking and thawing loss and FA composition...
April 26, 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/28317711/analysis-of-cannabinoids-by-liquid-chromatography-mass-spectrometry-in-milk-liver-and-hemp-seed-to-ensure-food-safety
#13
Úrsula Escrivá, María Jesús Andrés-Costa, Vicente Andreu, Yolanda Picó
A method for determining cannabinoids, Δ9-tetrahidrocannabinol (THC), 11-nor-9-carboxy-Δ(9)-THC (THC-COOH) and 11-hidroxy-Δ(9)-THC (THC-OH) in milk, liver and hemp seeds based on liquid chromatography tandem mass spectrometry has been optimized and validated. Analytes were extracted with methanol and the extracts cleaned-up by solid-phase extraction using Oasis HLB (60mg). The developed method was validated according to the Commission Decision 2002/657/EC. The decision limit (CCα) and detection capability (CCβ) ranged from 3...
August 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28240903/flexible-bionanocomposites-from-epoxidized-hemp-seed-oil-thermosetting-resin-reinforced-with-halloysite-nanotubes
#14
Peter S Shuttleworth, Ana M Díez-Pascual, Carlos Marco, Gary Ellis
Hemp seed (Cannabis sativa L.) oil comprises a variety of beneficial unsaturated triglycerides with well-documented nutritional and health benefits. However, it can become rancid over a relatively short time period, leading to increased industrial costs and waste of a valuable product. The development of sustainable polymers is presented as a strategy, where both the presence of unsaturation and peroxide content could be effectively used to alleviate both the waste and financial burden. After the reaction with peroxyacetic acid, the incorporation of halloysite nanotubes (HNTs), and the subsequent thermal curing, without the need for organic solvents or interfacial modifiers, flexible transparent materials with a low glass-transition temperature were developed...
March 8, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28224333/anti-neuroinflammatory-effects-of-grossamide-from-hemp-seed-via-suppression-of-tlr-4-mediated-nf-%C3%AE%C2%BAb-signaling-pathways-in-lipopolysaccharide-stimulated-bv2-microglia-cells
#15
Qian Luo, Xiaoli Yan, Larisa Bobrovskaya, Mei Ji, Huiqing Yuan, Hongxiang Lou, Peihong Fan
Grossamide, a representative lignanamide in hemp seed, has been reported to possess potential anti-inflammatory effects. However, the potential anti-neuroinflammatory effects and underlying mechanisms of action of grossamide are still unclear. Therefore, the present study investigated the possible effects and underlying mechanisms of grossamide against lipopolysaccharide (LPS)-induced inflammatory response in BV2 microglia cells. BV2 microglia cells were pre-treated with various concentrations of grossamide before being stimulated with LPS to induce inflammation...
April 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28188639/effect-of-unconventional-oilseeds-safflower-poppy-hemp-camelina-on-in-vitro-ruminal-methane-production-and-fermentation
#16
Shaopu Wang, Michael Kreuzer, Ueli Braun, Angela Schwarm
BACKGROUND: Dietary supplementation with oilseeds can reduce methane emission in ruminants, but only a few common seeds have been tested so far. This study tested safflower (Carthamus tinctorius), poppy (Papaver somniferum), hemp (Cannabis sativa), and camelina (Camelina sativa) seeds in vitro using coconut (Cocos nucifera) oil and linseed (Linum usitatissimum) as positive controls. RESULTS: All the tested oilseeds suppressed methane yield (mL g(-1) dry matter, up to 21%) compared to the non-supplemented control when provided at 70 g oil kg(-1) dry matter, and they were as effective as coconut oil...
February 11, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/27924117/transcriptome-differences-between-fiber-type-and-seed-type-cannabis-sativa-variety-exposed-to-salinity
#17
Jiajia Liu, Qin Qiao, Xia Cheng, Guanghui Du, Gang Deng, Mingzhi Zhao, Feihu Liu
The industrial hemp varieties 'Yunma 5' and 'Bamahuoma,' which demonstrate growth vigor and environmental adaptability, have been primarily cultivated in Yunnan and Guangxi, China, respectively, for fiber and seeds. The results of physiological measurements showed the phenotypic differences between the two varieties in response to salt stress. RNA-Seq analysis was first performed on leaves of both varieties sampled at four time intervals (0, 2, 4, 6 days) after treatment with salt (500 mM NaCl) We identified 220 co-up-regulated differentially expressed genes (DEGs) in the two varieties, while 26 up-regulated DEGs and 24 down-regulated DEGs were identified exclusively in the single varieties after 2 days of salt stress...
October 2016: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/27900531/accidental-cannabis-poisoning-in-the-elderly
#18
Anja Zupan Mežnar, Miran Brvar, Gregor Kralj, Dragan Kovačič
There are two main varieties of Cannabis sativa plant, namely, hemp which is cultivated for fiber and seeds and contains low amounts of psychoactive cannabinoids and the other which is cultivated for the drug cannabis or marijuana. Increasing popularity of hemp food products and alleged beneficial effects of cannabinoids in the drug variety of cannabis might lead to confusion in the lay population and unintentional ingestion of marijuana, as described in this case report. During a workshop on the use of hemp for medicinal purposes, there was a degustation of various hemp food products including also cookies that were brought by one of the attendees...
December 2016: Wiener Klinische Wochenschrift
https://www.readbyqxmd.com/read/27882891/a-comparative-mass-spectrometric-study-of-fatty-acids-and-metals-in-some-seed-extracts
#19
COMPARATIVE STUDY
Sonia Niculina Suvar, R Bleiziffer, P Podea, A Iordache, C Voica, R Zgavarogea, M Culea
A major cause of cardiovascular diseases and cancer is diet content, so the optimization of micronutrients in food is very important. Omega-3 fatty acids supplementation for patients had beneficial effects on subjective global assessment score and metabolic profiles. Fatty acids content and the metal ions in different seeds (e.g. linseed, poppy, grape, hemp, nuts, pumpkin, sesame, watermelon, chia) recommended as food supplements, purchased on the Romanian market, were compared. Gas chromatography coupled to mass spectrometry (GC-MS) was used as an excellent technique for fatty acids identification and quantitation, and inductively coupled plasma mass spectrometer (ICP-MS) for analytical measurements of metals...
2016: European Journal of Mass Spectrometry
https://www.readbyqxmd.com/read/27590030/determination-of-cannabinoids-in-hemp-nut-products-in-taiwan-by-hplc-ms-ms-coupled-with-chemometric-analysis-quality-evaluation-and-a-pilot-human-study
#20
Chih-Wei Chang, Chun-Wei Tung, Chin-Chuan Tsai, Yu-Tse Wu, Mei-Chich Hsu
Hemp nuts are mature cannabis seeds obtained after shelling and that are commonly used in traditional Chinese medicine for treating functional constipation. In this work, we screened hemp nut products, classified them, and verified the legality of consuming them. A total of 18 products were purchased from Taiwan, China, and Canada. Validated high-performance liquid chromatography with tandem mass spectrometry methods were developed for analyzing the cannabinoid (i.e., Δ(9) -tetrahydrocannabinol (THC), cannabidiol (CBD), and cannabinol) content of the products and the concentration of urinary 11-nor-9-carboxy-THC...
September 2, 2016: Drug Testing and Analysis
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