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Ashish Dhir, Emanuele Buratti, Maria A van Santen, Reinhard L├╝hrmann, Francisco E Baralle
Abundance of pseudo splice sites in introns can potentially give rise to innumerable pseudoexons, outnumbering the real ones. Nonetheless, these are efficiently ignored by the splicing machinery, a process yet to be understood completely. Although numerous 5' splice site-like sequences functioning as splicing silencers have been found to be enriched in predicted human pseudoexons, the lack of active pseudoexons pose a fundamental challenge to how these U1snRNP-binding sites function in splicing inhibition. Here, we address this issue by focusing on a previously described pathological ATM pseudoexon whose inhibition is mediated by U1snRNP binding at intronic splicing processing element (ISPE), composed of a consensus donor splice site...
February 17, 2010: EMBO Journal
Mariastefania Antica, Borka Kusic, Dubravka Hranilovic, Allan B Dietz, Stanimir Vuk-Pavlovic
We studied genes differentially transcribed during development of murine thymocytes. By the use of differential display of mRNA by polymerase chain reaction (DD-PCR) we identified a cDNA for U2snRNP-A' from a transcript abundant in precursor thymocytes, but rare in mature T cells. The transcript was fully cloned and found to be 97% homologous to the human cDNA for U2 snRNP-A'. We found the gene most abundantly transcribed on day 15 of gestation and in adult prothymocytes, spleen, testis and liver. Further characterization of snRNP proteins in the mouse is warranted in an effort to establish animal models of autoimmunity relevant for studies of connective tissue diseases or systemic lupus erythematosus, where patients harbor autoantibodies reactive to snRNP...
July 3, 2002: Immunology Letters
D Libri, N Graziani, C Saguez, J Boulay
The UAP56 gene has been shown to be required for prespliceosome assembly in mammals. We report here the isolation of the Schizosaccharomyces pombe ortholog of this gene by heterologous complementation of a combined PRP40HA(3)/nam8Delta defect in budding yeast. The Saccharomyces cerevisiae ortholog, YDL084w/SUB2, is also able to suppress this defect. We show that SUB2 is involved in splicing in vivo as well as in vitro. Sub2 defective extracts form a stalled intermediate that contains U2snRNP and can be chased into functional spliceosomes...
January 1, 2001: Genes & Development
J P O'Connor, J C Alwine, C S Lutz
Mouse monoclonal antibodies (MAbs) were generated against Escherichia coli-produced U1snRNP-A (U1A) protein. U1A-specific MAbs as well as MAbs that reacted with both U1A and U2snRNP-B" (U2B") were isolated. MAb 12E12 was unique among the characterized MAbs because it failed to immunoprecipitate U1A protein produced by in vitro transcription and translation using rabbit reticulocyte lysates. However, when U1A protein was made using a wheat germ extract, MAb 12E12 could immunoprecipitate U1A quite readily, as did the other MAbs...
December 1997: RNA
G G Simpson, P Vaux, G Clark, R Waugh, J D Beggs, J W Brown
U1 and U2snRNPs play key roles in pre-mRNA splicing. The interactions between the U1 and U2snRNP-specific proteins, U1A, U2A' and U2B'' and their respective UsnRNAs are of interest both to elucidate their roles in splicing, and as models to study RNA-protein interactions. We have cloned a full-length cDNA, encoding U2B'', from potato. This is the first report of a sequence for a plant UsnRNP protein. The plant U2B'' sequence exhibits extensive similarity with the human U2B'' protein at both the DNA and amino acid levels...
October 11, 1991: Nucleic Acids Research
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