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https://www.readbyqxmd.com/read/28646058/semen-collection-methods-in-alpacas
#1
REVIEW
M C Abraham, K de Verdier, R Båge, J M Morrell
South American camelids, particularly alpacas, are gaining in popularity outside their native lands. Reproductive biotechnologies, such as artificial insemination or embryo transfer with cryopreserved embryos, are more complicated in these species than many others due to differences in their reproductive physiology compared to the more commonly encountered domestic livestock. This article reviews the methods currently available for obtaining semen or spermatozoa from alpacas and describes some of the problems associated with handling the viscous seminal plasma characteristic of camelids...
June 24, 2017: Veterinary Record
https://www.readbyqxmd.com/read/28643872/circulating-microrna-as-candidates-for-early-embryonic-viability-in-cattle
#2
K G Pohler, J A Green, L A Moley, S Gunewardena, W T Hung, R R Payton, X Hong, L K Christenson, T W Geary, M F Smith
Blood-borne extracellular vesicles (i.e. exosomes and microvesicles) carrying microRNAs (miRNAs) could make excellent biomarkers of disease and different physiologic states, including pregnancy status. We tested the hypothesis that circulating extracellular vesicle-derived miRNAs might differentiate the pregnancy status of cows that had maintained pregnancy to Day 30 from non-pregnant cows or from those that exhibited embryonic mortality between Days 17-30 of gestation. Cows were randomly assigned for artificial insemination with fertile semen (n= 36) or dead semen (n = 8; control group) on Day 0 (day of estrus)...
June 23, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28642755/vaginal-and-uterine-bacterial-communities-in-postpartum-lactating-cows
#3
Brooke A Clemmons, Sydney T Reese, Felipe G Dantas, Gessica A Franco, Timothy P L Smith, Olusoji I Adeyosoye, Ky G Pohler, Phillip R Myer
Reproductive inefficiency in cattle has major impacts on overall productivity of cattle operations, increasing cost of production, and impacting the sustainability of the cattle enterprise. Decreased reproductive success and associated disease states have been correlated with the presence of specific microbes and microbial community profiles, yet details of the relationship between microbial communities and host physiology are not well known. The present study profiles and compares the microbial communities in the bovine uterus and vagina using 16S rRNA sequencing of the V1-V3 hypervariable region at the time of artificial insemination...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28628844/serum-micrornas-in-buffalo-cows-potential-biomarkers-of-pregnancy
#4
Gabriella Guelfi, Valentina Stefanetti, Silvio De Luca, Andrea Giontella, Vittoria Lucia Barile, Olimpia Barbato
MiRNAs (microRNA) constitute a large family of single-stranded, non-coding small RNAs. Although the functions and target genes of most miRNAs are still unknown, it has been well established that they are involved in embryogenesis, organogenesis and neonatal birth. In recent decades, interest in buffalo breeding has largely increased worldwide thus focusing the attention on this species as a dairy purpose animal. Problems related to long calving interval, late puberty and seasonal anestrus hamper reproductive efficiency in this species...
June 4, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28623008/yucca-schidigera-can-promote-rabbit-growth-fecundity-affect-the-release-of-hormones-in-vivo-and-in-vitro-induce-pathological-changes-in-liver-and-reduce-ovarian-resistance-to-benzene
#5
Martina Földešiová, Andrej Baláži, Ľubica Chrastinová, Juraj Pivko, Jan Kotwica, Abdel Halim Harrath, Peter Chrenek, Alexander V Sirotkin
This study evaluated the effect of Yucca schidigera (YS) extract on the physiological, reproductive, and endocrine indexes of New Zealand White rabbit does. Six-week-old rabbit does were fed a standard diet (control group) or a diet enriched with 5 or 20g of Y powder extract per 100-kg feed mixture for 350days. The does were artificially inseminated after induction of superovulation. Weight gain; conception and kindling rate; viability of pups and mothers; histopathological state of liver and muscle; plasma levels of progesterone (P4), oxytocin (OT), and prostaglandin F (PGF); and the release of P4, insulin-like growth factor I (IGF-I), OT, and PGF by isolated ovarian fragments and their response to the addition of benzene were analyzed...
June 10, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28620256/effects-of-season-and-artificial-photoperiod-on-semen-and-seminal-plasma-characteristics-in-bucks-of-two-goat-breeds-maintained-in-a-semen-collection-center
#6
Francisco Arrebola, José-Alfonso Abecia
AIM: This study quantified the effects of season and photoperiodic treatment on semen and seminal plasma (SP) characteristics in 12 bucks of two Spanish goat breeds (Murciano-Granadina, and Payoya) for the past 1 year. MATERIALS AND METHODS: A total of 6 bucks (three of each breed) were exposed to the natural day length and the other six males (three of each breed) were exposed to alternating conditions of 2 months of long days (16 h light) and 2 months of short days (8 h light)...
May 2017: Veterinary World
https://www.readbyqxmd.com/read/28620250/influence-of-clinical-mastitis-and-its-treatment-outcome-on-reproductive-performance-in-crossbred-cows-a-retrospective-study
#7
Narender Kumar, A Manimaran, M Sivaram, A Kumaresan, S Jeyakumar, L Sreela, P Mooventhan, D Rajendran
AIM: Evaluation of the effect of clinical mastitis (CM) and its treatment outcome on the reproductive performance in crossbred cows retrospectively. MATERIALS AND METHODS: Datasets of 835 lactating cows affected with CM during a period of 12 years (2001-2012) were considered for this study. Mastitis treatment related data and reproductive parameters such as days to first detected heat (DTFDH), days to first insemination (DTFI), days open (DO), and number of services per conception (SC) were collected from mastitis treatment and artificial insemination registers, respectively...
May 2017: Veterinary World
https://www.readbyqxmd.com/read/28608609/artificial-insemination-with-frozen-thawed-boar-sperm
#8
REVIEW
Marc Yeste, Joan E Rodríguez-Gil, Sergi Bonet
Artificial insemination with frozen-thawed semen in pigs is not a routine technique; its use is restricted to specific cases, such as preservation of valuable genetic material (germplasm banks), safety strategies in case of natural disasters, long-distance transport of sperm, and in combination with sex-sorting. Cryoinjuries resulting from freeze-thawing protocols are a major concern with regard to the fertilization capacity of the treated sperm, which is lower than that of liquid-stored semen. Here we provide an overview of artificial insemination using cryopreserved sperm, and summarize the factors that influence cryopreservation success before, during, and after freeze-thaw (i...
June 13, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28608308/effect-of-reproductive-methods-and-gnrh-administration-on-long-term-protocol-in-santa-ines-ewes
#9
Marcos V Biehl, Marcos V C Ferraz Junior, Evandro M Ferreira, Daniel M Polizel, Alexandre A Miszura, José P R Barroso, Gabriela B Oliveira, Analisa V Bertoloni, Alexandre V Pires
This study aimed to determine whether reproductive performance of ewes submitted to laparoscopic timed artificial insemination (TAI) would be similar to ante meridiem (AM)/post meridiem (PM) rule and assisted natural mating (NM), and whether GnRH may enhance the pregnancy rate in TAI. In experiment I, 191 non-lactating ewes were synchronized, then TAI was performed either 48 h after progesterone (P4) removal (TAI-48 h) or 12 h after estrus detection (AM/PM); moreover, some ewes were submitted to NM (NM) as control treatment...
June 12, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28601161/effective-use-of-sexedultra%C3%A2-sex-sorted-semen-for-timed-artificial-insemination-of-beef-heifers
#10
J M Thomas, J W C Locke, R Vishwanath, J B Hall, M R Ellersieck, M F Smith, D J Patterson
An experiment was designed to evaluate the relative fertility of SexedULTRA(TM) sex-sorted semen compared to conventional, non-sex-sorted semen when used among beef heifers in conjunction with split-time AI following the 14-d CIDR-PG protocol. Units of conventional semen were generated with 25.0 × 10(6) live cells per 0.5 ml straw prior to freezing, and units of sex-sorted semen were generated using the SexedULTRA(TM) Genesis III sorting technology with 4.0 × 10(6) live cells per 0.25 ml straw prior to freezing...
August 2017: Theriogenology
https://www.readbyqxmd.com/read/28601153/equine-chorionic-gonadotropin-increases-fertility-of-grazing-dairy-cows-that-receive-fixed-time-artificial-insemination-in-the-early-but-not-later-postpartum-period
#11
Alexandre B Prata, Guilherme C S Pontes, Pedro Leopoldo J Monteiro, Jéssica N Drum, Milo C Wiltbank, Roberto Sartori
This study evaluated effects of equine chorionic gonadotropin (eCG) on fertility of 679 crossbred (Bos taurus x Bos indicus) lactating grazing cows synchronized for fixed-time AI (FTAI). At a random day of the estrous cycle cows received an intravaginal progesterone (P4) implant, 2 mg estradiol benzoate (EB) and 100 μg gonadorelin (D0-AM). On D7-AM, cows received 0.5 mg sodium cloprostenol and were randomly assigned into two treatments: eCG (n = 340; 400 IU eCG on D7), or Control (n = 339; no eCG). On D8-PM, P4 implants were removed and cows received 0...
August 2017: Theriogenology
https://www.readbyqxmd.com/read/28601146/genetic-component-of-sensitivity-to-heat-stress-for-nonreturn-rate-of-brazilian-holstein-cattle
#12
M L Santana, A B Bignardi, G Stefani, L El Faro
The objectives of the present study were: 1) to investigate variation in the genetic component of heat stress for nonreturn rate at 56 days after first artificial insemination (NR56); 2) to identify and characterize the genotype by environment interaction (G × E) due to heat stress for NR56 of Brazilian Holstein cattle. A linear random regression model (reaction norm model) was applied to 51,748 NR56 records of 28,595 heifers and multiparous cows. The decline in NR56 due to heat stress was more pronounced in milking cows compared to heifers...
August 2017: Theriogenology
https://www.readbyqxmd.com/read/28583611/pregnancy-associated-glycoproteins-pags-concentrations-in-water-buffaloes-bubalus-bubalis-during-gestation-and-the-postpartum-period
#13
O Barbato, L Menchetti, N M Sousa, A Malfatti, G Brecchia, C Canali, J F Beckers, V L Barile
For the first time in literature this study describes the pregnancy-associated glycoprotein (PAG) profile of buffalo cows during gestation and the post-partum period using antiserum raised against PAG-molecules purified from buffalo placenta (AS#860). Ninety-eight buffalo cows, belonging to a buffalo herd subjected to a synchronization and artificial insemination (AI) program, were enrolled in this study. Blood samples were taken on days 0 (AI), 23, 25, 28, 30 and then biweekly until the end of pregnancy. Pregnancy was confirmed by ultrasonography on days 28 and 45, and by rectal palpation from day 60 onwards...
July 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28583598/detection-of-macrophages-in-rabbit-semen-and-their-relationship-with-semen-quality
#14
Lenka Kuželová, Jaromír Vašíček, Ján Rafay, Peter Chrenek
We aimed at the evaluating the occurrence of macrophages in rabbit semen and finding possible relationship between macrophage concentration and spermatozoa quality. The concentration of macrophages in semen samples from broiler rabbit males of lines M91 and P91 (n = 30) without overt evidence of genital tract infections was determined using monocyte/macrophage lineage antigen CD14 and flow cytometry. Then the rabbits were assigned into three groups according to the macrophage concentration in semen (MΦ1 group with less than 1 × 10(6) macrophages/mL, MΦ2 group with 1...
July 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28583595/the-copper-transporter-slc31a1-ctr1-is-expressed-in-bovine-spermatozoa-and-oocytes-copper-in-ivf-medium-improves-sperm-quality
#15
J P Anchordoquy, J M Anchordoquy, A M Pascua, N Nikoloff, P Peral-García, C C Furnus
Adequate dietary intake of copper (Cu) is required for normal reproductive performance in cattle. The objective of this study was to investigate the pregnancy rates from cattle with deficient, marginal and adequate Cu plasma concentration at the beginning of artificial insemination protocol. Moreover, we determined Cu concentrations present in bovine oviductal fluid (OF), and the effects of Cu on fertilizing ability of bovine spermatozoa. Also, the presence of Cu transporter, SLC31A1 (also known as CTR1), in spermatozoa and in vitro matured oocyte were investigated...
July 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28583529/association-between-the-fertile-period-and-live-birth-post-kidney-transplantation-a-retrospective-single-center-cohort-study
#16
Y Itabashi, Y Ohashi, M Muramatsu, T Kawamura, Y Hyodo, Y Hamasaki, S Shishido, K Sakai, T Maemura, M Morita, A Aikawa
BACKGROUND: Despite restoration of fertility after kidney transplantation, the benefit is limited in female kidney recipients. Our objective is to determine the reasons for this discrepancy. METHODS: We evaluated 315 women who underwent kidney transplantation from 1983 to 2015 (a median of age at transplantation [10th-90th percentile] of 32 years [7-55 years]); 230 recipients between the ages of 15 to 49 years old as of March 2016 were observed. RESULTS: We experienced 10 abortions and 21 live births from our 23 recipients and 2 abortions and 7 live births in 7 recipients from other transplant center...
June 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/28578791/letrozole-an-aromatase-inhibitor-reduces-post-peak-age-related-regression-of-rooster-reproductive-performance
#17
Emad Abdulgabbar Ali, Mahdi Zhandi, Armin Towhidi, Mojtaba Zaghari, Mahdi Ansari, Mojtaba Najafi, Hamid Deldar
This study was designed to evaluate orally administrated Letrozole (Lz) on reproductive performance, plasma testosterone and estradiol concentrations and relative abundance of mRNA of GnRH, FSH and LH in roosters. Ross 308 roosters (n=32) that were 40-weeks of age were individually housed and received a basal standard diet supplemented different amounts of capsulated Lz [0 (Lz-0), 0.5 (Lz-0.5), 1 (Lz-1) or 1.5 (Lz-1.5), mg Lz/bird/day] for 12 weeks. Sperm quality variables and plasma testosterone and estradiol concentrations were assessed from the first to the tenth week of the treatment period...
May 27, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28576343/economic-assessment-of-ostertagia-ostertagi-and-fasciola-hepatica-infections-in-dairy-cattle-herds-in-germany-using-paracalc-%C3%A2
#18
Jane Fanke, Johannes Charlier, Torsten Steppin, Georg von Samson-Himmelstjerna, Jozef Vercruysse, Janina Demeler
The aim of the current study was to estimate economic costs of Ostertagia ostertagi and Fasciola hepatica infections in dairy cattle herds in Germany using the online calculation programme Paracalc(®). Following a questionnaire, survey data were available from 464 farms in 14 federal states. On those farms bulk tank milk (BTM) samples and additionally up to six serum samples collected from first season grazing calves were analysed, using a commercially available ELISA (Boehringer Ingelheim SVANOVA Biotech AB, Uppsala, Sweden), an in-house ELISA (F...
June 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28571986/characterization-of-the-variability-and-repeatability-of-gonadotropin-releasing-hormone-induced-luteinizing-hormone-responses-in-dairy-cows-within-a-synchronized-ovulation-protocol
#19
M Gobikrushanth, P A Dutra, T C Bruinjé, M G Colazo, S T Butler, D J Ambrose
The primary objective was to determine the variability and repeatability of GnRH-induced LH responses. The secondary objective was to evaluate the associations among plasma LH, FSH, estradiol (E2), and progesterone (P4) concentrations. One hundred lactating Holstein cows (35 primiparous, 65 multiparous) were initially subjected to a presynchronization protocol (d 0, PGF2α; d 3, GnRH) followed 7 d later by Ovsynch (d 10, GnRH; d 17, PGF2α; 56 h later, GnRH) and timed artificial insemination 16 h after the last GnRH...
May 29, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28551196/estimation-of-genetic-parameters-and-heterosis-for-longevity-in-crossbred-danish-dairy-cattle
#20
J B Clasen, E Norberg, P Madsen, J Pedersen, M Kargo
Crossbreeding has been shown to improve the longevity of dairy cattle in countries across the world. The aim of this study was to estimate heterosis, breed effects, and genetic parameters for longevity in crossbred dairy cattle among Danish Holstein (DH), Danish Red (DR), and Danish Jersey (DJ) breeds. Data were provided from 119 Danish commercial herds that use systematic crossbreeding (i.e., rotational crossbreeding). Additional data from 11 mixed-breed herds with DH and DJ were included to estimate reliable breed effects for DJ...
May 24, 2017: Journal of Dairy Science
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