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Artificial insemination

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https://www.readbyqxmd.com/read/27913974/incorporation-of-estradiol-benzoate-to-cidr-protocol-improves-the-reproductive-responses-in-crossbred-dairy-heifers
#1
Muhammad Usman Mehmood, Ahmad Qamar, Abdul Sattar, Liaquat Ahmad, Nasim Ahmad
The present study was designed to determine the effect of estradiol benzoate (EB) on reproductive response following a controlled internal drug release (CIDR) protocol in crossbred (Sahiwal × Friesian) dairy heifers. In the first trial, a total of 100 crossbred dairy heifers were treated with CIDR protocol for 7 days and injected with the PGF2α on day 6. After 24 h of CIDR removal, one group (EB = 50) was injected with estradiol benzoate whereas the other (control = 50) remained untreated. Estrus intensity and response were recorded visually and ovulation rate was recorded by ultrasonography...
December 2, 2016: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/27908687/fertility-response-evaluation-of-artificial-insemination-methods-in-sheep-with-fresh-and-frozen-thawed-semen
#2
Reza Masoudi, Ahmad Zare Shahneh, Armin Towhidi, Hamid Kohram, Abbas Akbarisharif, Mohsen Sharafi
The aim of this study was to evaluate the fertility response of artificial insemination (AI) methods with fresh and frozen sperm in sheep. In experiment 1, one hundred and fifty fat tailed Zandi ewes were assigned into 3 equal groups and inseminated with three AI methods consisting of vaginal, laparoscopic and trans-cervical AI with fresh semen. In experiment 2, a factorial study (3 AI methods × 2 extenders) was used to analyze the effects of three AI methods and two freezing extenders containing soybean lecithin (SL) or Egg yolk (EY) on reproductive performance of 300 fat tailed Zandi ewes...
November 28, 2016: Cryobiology
https://www.readbyqxmd.com/read/27905875/a-nonsense-mutation-in-the-col7a1-gene-causes-epidermolysis-bullosa-in-vorderwald-cattle
#3
Hubert Pausch, Simon Ammermüller, Christine Wurmser, Henning Hamann, Jens Tetens, Cord Drögemüller, Ruedi Fries
BACKGROUND: The widespread use of individual sires for artificial insemination promotes the propagation of recessive conditions. Inadvertent matings between unnoticed carriers of deleterious alleles may result in the manifestation of fatal phenotypes in their progeny. Breeding consultants and farmers reported on Vorderwald calves with a congenital skin disease. The clinical findings in affected calves were compatible with epidermolysis bullosa. RESULTS: Pedigree analysis indicated autosomal recessive inheritance of epidermolysis bullosa in Vorderwald cattle...
December 1, 2016: BMC Genetics
https://www.readbyqxmd.com/read/27898968/post-artificial-insemination-supplementation-with-calcium-salts-of-soybean-oil-influences-pregnancy-establishment-factors-in-beef-cows
#4
R S Cipriano, R F Cooke, A D Rodrigues, L G T Silva, D W Bohnert, R S Marques, J L M Vasconcelos, A V Pires, R L A Cerri
The objective of this experiment was to compare hormonal, uterine, and conceptus factors associated with pregnancy establishment in beef cows supplemented or not with Ca salts of soybean oil (CSSO) for 21 d beginning after timed AI. One hundred lactating multiparous Nelore cows were allocated to 20 groups of 5 cows/group and timed inseminated on d 0 of the experiment. After AI, groups were randomly assigned to receive (as-fed basis) 100 g of protein-mineral mix + 100 g of ground corn per cow per day, in addition to 1) 100 g/cow daily of CSSO ( = 10) or 2) 100 g/cow daily of kaolin (CON; rumen-inert indigestible substance; = 10)...
November 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/27898921/using-estrus-detection-patches-to-optimally-time-insemination-improved-pregnancy-risk-in-suckled-beef-cows-enrolled-in-a-fixed-time-artificial-insemination-program
#5
S L Hill, D M Grieger, K C Olson, J R Jaeger, C R Dahlen, G A Bridges, F Dantas, J E Larson, A M Muth-Spurlock, J K Ahola, M C Fischer, G A Perry, E L Larimore, T L Steckler, W D Whittier, J F Currin, J S Stevenson
A multilocation study examined pregnancy risk (PR) after delaying AI in suckled beef cows from 60 to 75 h when estrus had not been detected by 60 h in response to a 7-d CO-Synch + progesterone insert (CIDR) timed AI (TAI) program (d -7: CIDR insert concurrent with an injection of GnRH; d 0: PGF injection and removal of CIDR insert; and GnRH injection at TAI [60 or 75 h after CIDR removal]). A total of 1,611 suckled beef cows at 15 locations in 9 states (CO, IL, KS, MN, MS, MT, ND, SD, and VA) were enrolled...
September 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/27898908/gonadotropin-releasing-hormone-increased-pregnancy-risk-in-suckled-beef-cows-not-detected-in-estrus-and-subjected-to-a-split-time-artificial-insemination-program
#6
S L Hill, D M Grieger, K C Olson, J R Jaeger, C R Dahlen, M R Crosswhite, N Negrin Pereira, S R Underdahl, B W Neville, J Ahola, M C Fischer, G E Seidel, J S Stevenson
We hypothesized that GnRH would increase pregnancy risk (PR) in a split-time AI program for cows in which estrus was not detected. A total of 1,236 suckled beef cows at 12 locations in 3 states (Colorado, Kansas, and North Dakota) were enrolled. Before applying the fixed-time AI program, BCS was assessed. Cows were treated on d -7 with a progesterone insert concurrent with 100 μg GnRH and on d 0 with 25 mg PGF plus removal of the insert. Estrus-detection patches were affixed to cows at insert removal. Estrus was defined to have occurred when an estrus-detection patch was >50% colored (activated)...
September 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/27898863/rapid-communication-expression-of-an-endogenous-retroviral-element-during-early-gestation-in-beef-heifers
#7
K J McLean, M S Crouse, M R Crosswhite, D N Black, C R Dahlen, P P Borowicz, L P Reynolds, A K Ward, B W Neville, J S Caton
Endogenous retroviral gene elements have been implicated in development and formation of the feto-maternal interface. A variant of the syncytin endogenous retroviral envelope gene family, , was recently found in ruminants. We hypothesized that mRNA would be differentially expressed in utero-placental tissues and would fluctuate during key time points of early gestation in beef heifers. Commercial Angus crossbred heifers ( = 46; ∼15 mo of age; BW = 362.3 ± 34.7kg) housed in 6-animal pens were fed daily with native grass hay and supplemented with cracked corn to gain 0...
October 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/27890022/continuous-light-after-2-months-of-long-days-stimulates-ram-testis-volume-and-increases-fertility-in-spring
#8
D Chesneau, D Guillaume, P Chemineau, B Malpaux
Seasonal reproduction is one of the major biotechnical and economic constraints of sheep production in temperate latitudes. Treatments using extra light followed by melatonin implants have been used satisfactorily in open barns, farms and artificial insemination centres to produce out-of-season sexual activity in rams. The aim of the present study is to explore the possibility of replacing melatonin implants with continuous light (LL), which was recently shown to increase LH secretion similar to melatonin and/or pinealectomy...
November 28, 2016: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/27889127/a-comparison-of-semen-diluents-on-the-in-vitro-and-in-vivo-fertility-of-liquid-bull-semen
#9
Edel M Murphy, Craig Murphy, Ciara O'Meara, Gemma Dunne, Bernard Eivers, Patrick Lonergan, Sean Fair
The aim of this study was to assess the effect of semen diluent on calving rate (CR) following artificial insemination with liquid bull semen stored for up to 3 d postcollection. In experiment 1, the effect of storing liquid semen maintained at a constant ambient temperature in 1 of 7 different diluents [Caprogen (homemade), OptiXcell, BioXcell, BullXcell, INRA96, NutriXcell, or AndroMed (all commercially available)] on total and progressive motility was assessed on d 0, 1, 2, and 3 postcollection. In experiment 2, the field fertility of liquid semen diluted in Caprogen, BioXcell, or INRA96 and inseminated on d 1, 2, or 3 postcollection was assessed in comparison to frozen-thawed semen (total of n = 19,126 inseminations)...
November 23, 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/27885724/identification-of-lactoferrin-and-glutamate-receptor-interacting-protein-1-in-bovine-cervical-mucus-a-putative-marker-for-oestrous-detection
#10
W Y Lee, M H Park, K W Kim, H Song, K B Kim, C S Lee, N K Kim, J K Park, B C Yang, K B Oh, G S Im, H J Chung
Accurate detection of oestrus is important for artificial insemination. The aim of this study was to identify oestrous-specific bovine cervical mucus proteins that could be used to determine the optimal time for artificial insemination. Non-oestrous and controlled internal drug release (CIDR)-induced oestrous-stage mucus proteins were purified and subjected to surface-enhanced laser desorption/ionization time-of-flight mass spectrometry, sodium dodecyl sulphate polyacrylamide gel electrophoresis and MALDI-TOF/TOF...
November 25, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27881083/loss-of-maternal-annexin-a10-via-a-34-kb-deleted-type-copy-number-variation-is-associated-with-embryonic-mortality-in-japanese-black-cattle
#11
Shinji Sasaki, Takayuki Ibi, Takayuki Akiyama, Moriyuki Fukushima, Yoshikazu Sugimoto
BACKGROUND: Conception is a fundamental trait for successful cattle reproduction. However, conception rates in Japanese Black cattle have been gradually declining over the last two decades. Although conception failures are mainly caused by embryonic mortality, the role of maternal genetic factors in the process remains unknown. Copy number variation (CNV), defined as large-scale genomic structural variants, contributes to several genetic disorders. To identify CNV associated with embryonic mortality in Japanese Black cattle, we evaluated embryonic mortality as a categorical trait with a threshold model and conducted a genome-wide CNV association study for embryonic mortality using 791 animals...
November 24, 2016: BMC Genomics
https://www.readbyqxmd.com/read/27878881/beneficial-effect-of-extracellular-adenosine-5-triphosphate-treatment-on-the-indochinese-leopard-panthera-pardus-delacouri-sperm-quality-after-cryopreservation
#12
P Thuwanut, W Tipkantha, B Siriaroonrat, P Comizzoli, K Chatdarong
The Indochinese leopard (Panthera pardus delacouri) population, included in CITES Appendix I, has been declining for decades. Proper gamete preservation condition is critical for breeding programme management using artificial insemination or in vitro fertilization (IVF). The present study aimed at investigating the impact of post-thawing treatment of leopard semen with extracellular adenosine 5'-triphosphate (ATPe) on sperm quality (including morphological traits and ability to fertilize an oocyte). Semen from six adult male leopards was collected by electroejaculation (one ejaculation per cat)...
November 22, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27872723/a-human-reproductive-approach-to-the-study-of-infertility-in-chimpanzees-an-experience-at-leon-s-zoological-park-mexico
#13
Raul Eduardo Piña-Aguilar, Janet López-Saucedo, Lilia Ivone Ruiz-Galaz, José de Jesús Barroso-Padilla, Mayra Celina Gallegos-Rivas, Claudia González-Ortega, Antonio Martin Gutiérrez-Gutiérrez
Great apes are mammals close to humans in their genetic, behavioral, social and evolutionary characteristics and new genomic information is revolutionizing our understanding of evolution in primates. However, all these species are endangered. While there are many global programs to protect these species, the International Union for Conservation of Nature (IUCN) projects that in a near future the wild populations will decrease significantly. Nowadays, the relevance of captive populations of great apes is becoming critical for research and understanding of pathophysiology of diseases...
2016: Veterinary Research Forum
https://www.readbyqxmd.com/read/27865501/cytological-endometritis-at-artificial-insemination-in-dairy-cows-prevalence-and-effect-on-pregnancy-outcome
#14
O Bogado Pascottini, M Hostens, P Sys, P Vercauteren, G Opsomer
The aims of our field study in dairy cows were (1) to consolidate cytotape (CT) as a valid technique to diagnose cytological endometritis (CYTO) during artificial insemination (AI); (2) to establish a cutoff point concerning the polymorphonuclear cells (PMN) proportion to diagnose CYTO at AI; (3) to assess the prevalence of CYTO at AI; and (4) to evaluate the effect of CYTO on the pregnancy outcome of that AI. The investigation was performed using 1,625 AI-CT samples harvested from 873 Holstein-Friesian cows from 18 dairy farms in the Flemish region of Belgium...
November 16, 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/27865409/high-somatic-cell-counts-and-changes-in-milk-fat-and-protein-contents-around-insemination-are-negatively-associated-with-conception-in-dairy-cows
#15
Ahmad Albaaj, Gilles Foucras, Didier Raboisson
The fertility of dairy cows has decreased dramatically worldwide over the last few decades, and several causes of this trend have been reported. Several studies have associated compromised udder health with deteriorating reproduction performance. Subclinical ketosis (SCK) has also been reported to be a risk factor for decreased conception. The objective of the present study was to describe how SCK might interact with the reported association between udder health and conception in dairy cows. Data from the French Milk Control Program and data on 8,549,667 instances of artificial insemination (AI) and their corresponding preceding and subsequent test-days from 5,979,701 Holstein cows were examined over a 5-year period (2008-2012)...
January 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/27863702/detecting-asymptomatic-rams-infected-with-mycoplasma-agalactiae-in-ovine-artificial-insemination-centers
#16
Miranda Prats-van der Ham, Juan Tatay-Dualde, Christian de la Fe, Ana Paterna, Antonio Sánchez, Juan Carlos Corrales, Antonio Contreras, Ángel Gómez-Martín
Mycoplasma agalactiae (Ma) is the main causative agent of ovine contagious agalactia, which is a serious disease of small ruminants. In endemic areas, its most common clinical situation consists of chronically infected herds, and asymptomatic infected individuals represent an epidemiological risk regarding the transmission of this disease. The aim of this work was to detect the presence of asymptomatic rams infected with Ma in different artificial insemination centers, and to determine the most effective way to identify these individuals so as to implement adequate surveillance protocols...
September 15, 2016: Theriogenology
https://www.readbyqxmd.com/read/27862440/fertility-of-angus-cross-beef-heifers-after-gnrh-treatment-on-day-23-and-timing-of-insemination-in-14-day-cidr-protocol
#17
R K Kasimanickam, J B Hall, W D Whittier
This study compared artificial insemination pregnancy rate (AI-PR) between 14-day CIDR-GnRH-PGF2α-GnRH and CIDR-PGF2α-GnRH synchronization protocol with two fixed AI times (56 or 72 hr after PGF2α). On day 0, heifers (n = 1311) from nine locations assigned body condition score (BCS: 1, emaciated; 9, obese), reproductive tract score (RTS: 1, immature, acyclic; 5, mature, cyclic) and temperament score (0, calm; and 1, excited) and fitted with a controlled internal drug release (CIDR, 1.38 g of progesterone) insert for 14 days...
November 14, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27859701/uterine-infection-with-bovine-herpesvirus-type-4-in-dairy-cows
#18
S Klamminger, I Prunner, M J Giuliodori, M Drillich
Diseases of the reproductive tract are a frequent problem in dairy herds. Herpesviruses are uterine pathogens also involved in other clinical diseases; for example, bovine herpesvirus type 4 BoHV-4 induces abortion, enteritis, metritis, pneumonia and vaginitis, but it can also be detected in healthy cows. The role of BoHV-4 in the development of clinical endometritis (CE) or subclinical endometritis (SE) has not clearly been described. Therefore, the objective of this study was to describe the prevalence of uterine BoHV-4 infection and its relationship with clinical, bacteriological and cytological findings in dairy cows 20-30 days after calving...
November 14, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27856784/usefulness-of-an-injectable-anaesthetic-protocol-for-semen-collection-through-urethral-catheterisation-in-domestic-cats
#19
Maria Carmela Pisu, Patrizia Ponzio, Chiara Rovella, Michela Baravalle, Maria Cristina Veronesi
OBJECTIVES: Although less often requested in comparison with dogs, the collection of semen in cats can be necessary for artificial insemination, for semen evaluation in tom cats used for breeding and for semen storage. Urethral catheterisation after pharmacological induction with medetomidine has proved to be useful for the collection of semen in domestic cats. However, most of the previously used protocols require the administration of high doses of medetomidine that can increase the risk of side effects, especially on the cardiovascular system...
November 17, 2016: Journal of Feline Medicine and Surgery
https://www.readbyqxmd.com/read/27846734/reproductive-technologies-and-the-risk-of-congenital-heart-defects
#20
Satoru Iwashima, Takamichi Ishikawa, Hiroaki Itoh
Assisted reproductive technology (ART) has become widespread, accounting for ∼2% of all births worldwide, with a similar proportion in Japan. Our goal was to determine whether ART is associated with an increased risk of congenital heart defect (CHD). The study subjects were 2716 pregnant women (2317 in a spontaneous conception [SC] group and 399 in an assisted conception [AC] group). Of patients in the AC group, 142 were treated with ovulation-inducing agents (OIAs), 56 with artificial insemination by the husband (AIH), 159 with in vitro fertilization (IVF), and 42 with intracytoplasmic sperm injection (ICSI)...
November 16, 2016: Human Fertility: Journal of the British Fertility Society
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