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Artificial insemination

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https://www.readbyqxmd.com/read/29777964/protection-of-gnrh-analogue-by-chitosan-dextran-sulfate-nanoparticles-for-intravaginal-application-in-rabbit-artificial-insemination
#1
L Casares-Crespo, P Fernández-Serrano, M P Viudes-de-Castro
The present study was designed to prove new rabbit insemination extenders containing aminopeptidase inhibitors (AMIs) with or without chitosan (CS)-dextran sulfate (DS) nanoparticles entrapping the GnRH analogue. In addition, different hormone concentrations were tested in these extenders, evaluating their in vivo effect on rabbit reproductive performance after artificial insemination. A total of 911 females were inseminated with semen diluted with the four experimental extenders (C4 group: 4 μg buserelin/doe in control medium (Tris-citric acid-glucose supplemented with bestatin 10 μM and EDTA 20 mM), C5 group: 5 μg of buserelin/doe in control medium, Q4 group: 4 μg of buserelin/doe into CS-DS nanoparticles in control medium, Q5 group: 5 μg of busereline/doe into CS-DS nanoparticles in control medium)...
May 16, 2018: Theriogenology
https://www.readbyqxmd.com/read/29769076/insemination-with-border-disease-virus-infected-semen-results-in-seroconversion-in-cows-but-not-persistent-infection-in-fetuses
#2
Ueli Braun, Fredi Janett, Sandra Züblin, Michèle von Büren, Monika Hilbe, Reto Zanoni, Matthias Schweizer
BACKGROUND: This study examined various health variables in cows after artificial insemination with Border disease virus (BDV)-infected semen and the occurrence of persistent infection in ensuing fetuses. Five cows were inseminated (day 0) with BDV-infected semen as well as with semen from a fertile Eringer bull. One cow, inseminated with virus-free semen only, served as a control. Clinical examination, assessment of eating and rumination activities, measurement of intraruminal temperature and leukocyte count were used to monitor the health of the cows...
May 16, 2018: BMC Veterinary Research
https://www.readbyqxmd.com/read/29767722/influence-of-bull-age-ejaculate-number-and-season-of-collection-on-semen-production-and-sperm-motility-parameters-in-holstein-friesian-bulls-in-a-commercial-artificial-insemination-centre
#3
Edel M Murphy, Alan K Kelly, Ciara O'Meara, Bernard Eivers, Patrick Lonergan, Sean Fair
In the current era of genomic selection, there is an increased demand to collect semen from genomically selected sires at a young age. The objective of this study was to assess the effect of bull age, ejaculate number, and season of collection on semen production (ejaculate volume, sperm concentration, and total sperm number; TSN) and sperm motility (prefreeze and post-thaw total and gross motility) parameters in Holstein Friesian bulls in a commercial artificial insemination (AI) center. The study involved the interrogation of a large dataset collected over a 4-yr period, (n = 8,983 ejaculates; n = 176 Holstein Friesian bulls aged between 9 mo and 8 yr)...
May 14, 2018: Journal of Animal Science
https://www.readbyqxmd.com/read/29763785/exposure-to-progesterone-previous-to-the-protocol-of-ovulation-synchronization-increases-the-follicular-diameter-and-the-fertility-of-suckled-bos-indicus-cows
#4
L M S Simões, R E Orlandi, J P M Massoneto, L A Scandiuzzi, B G Freitas, M R Bastos, J C Souza, J N S Sales
The objective was to evaluate the effect of injectable progesterone previous to the timed artificial insemination (TAI) protocol on follicular growth, ovulation and pregnancy rate of suckled Bos indicus cows. In experiment 1, 10 days before the beginning to TAI protocol (D-10), 431 suckled-anestrus Nelore cows (249 multiparous and 182 primiparous), were allocated to one of three treatments groups (control, P4i and P4iGnRH). At this moment, cows in the P4i and P4iGnRH group received 150 mg of injectable progesterone intramuscularly (Sincrogest injetável® , Ouro Fino, Brazil)...
May 3, 2018: Theriogenology
https://www.readbyqxmd.com/read/29762475/growth-hormone-secretion-patterns-in-german-landrace-dl-fetuses-and-piglets-compared-to-dl-piglets-with-inherited-1-25-dihydroxyvitamin-d3-deficiency
#5
Manfred Mielenz, Michael W Pfaffl, Christina Schlumbohm, Johein Harmeyer, Nahid Parvizi
The regulation of growth hormone (GH) release during prenatal development and during early postnatal life is not entirely clarified. In this study plasma GH concentrations in pigs with inherited pseudo vitamin D deficiency type I (PDDR-I), which regularly show growth retardation, were compared during ontogeny with unaffected pigs of the same breed (German Landrace, DL) as control. Plasma GH concentrations were measured in plasma of chronically catheterized fetuses (beginning on day 101 after mating or after artificial insemination) and in piglets (day 37 postpartum (p...
May 15, 2018: Nutrients
https://www.readbyqxmd.com/read/29753469/isolation-of-mycoplasma-spp-and-serological-responses-in-bulls-prior-to-and-following-their-introduction-into-mycoplasma-bovis-infected-dairy-herds
#6
M S Hazelton, J M Morton, K L Bosward, P A Sheehy, A M Parker, C J Dwyer, P G Niven, J K House
With the common use of bulls for breeding following a period of artificial insemination in seasonally bred dairy herds, it is important to consider the potential role of the bull in transmission of Mycoplasma spp. within and between herds. This study aimed to assess the prevalence of Mycoplasma spp. in a population of bulls before and after use in Mycoplasma bovis-infected herds. The frequency of subclinical infection was also measured serologically postbreeding, and the association of Mycoplasma spp. on semen quality was evaluated...
May 9, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29747159/seminal-plasma-influences-the-fertilizing-potential-of-cryopreserved-stallion-sperm
#7
Essraa M Al-Essawe, Margareta Wallgren, Manuela Wulf, Christine Aurich, Beatriz Macías-García, Ylva Sjunnesson, Jane M Morrell
Seminal plasma (SP) contains proteins that may influence cryosurvival and prevent capacitation-like changes due to freezing and thawing. The objective of this study was to investigate the effect of adding pooled SP from "good" (GF) or "bad" (BF) freezer stallions on sperm cells' fertilizing ability. "Good freezers" refers to stallions that usually produce ejaculates which can withstand cryopreservation, whilst "bad freezer" stallions produce ejaculates which cannot tolerate the freezing process...
April 26, 2018: Theriogenology
https://www.readbyqxmd.com/read/29740882/differential-gene-expression-of-eph-ephrin-a1-and-lepr-lep-with-high-or-low-number-of-embryos-in-pigs-during-implantation
#8
Y Fu, R V Knox, L Li, S Ren
The objective of this study was to ascertain whether mRNA and protein expressions of implantation-related genes (erythropoietin-producing hepatocellular receptor-ligand A1, Eph-ephrin A1 and leptin receptor-leptin, LEPR-LEP) differed between pigs with high and low number of embryos, and whether these differences in gene expression might affect embryo implantation. Experimental pig groups (n = 24) for high and low number of embryos were prepared by altering the number of eggs ovulated in pre-pubertal gilts treated with 1...
May 8, 2018: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29735243/effect-of-semen-processing-methods-on-lumpy-skin-disease-virus-status-in-cryopreserved-bull-semen
#9
C Henry Annandale, Mario P Smuts, Karen Ebersohn, Lizette du Plessis, Estelle H Venter, Tom A E Stout
Lumpy skin disease is an economically important disease of cattle, caused by the lumpy skin disease virus (LSDV; Capripoxvirus). It has a variable clinical appearance but, in severely affected animals, is associated with extensive skin damage, pneumonia and death. The LSDV can be found in the semen of infected bulls for prolonged periods of time, from where it can be transmitted by mating or artificial insemination and cause clinical disease in heifers and cows. In this study, an ejaculate was collected from a LSDV seronegative bull and confirmed free from LSDV DNA by PCR...
April 30, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29734443/effect-of-dilution-on-cryosurvival-of-low-sperm-doses-a-review
#10
S A Lone, T K Mohanty, M Bhakat, R K Baithalu, R Kumar
  BACKGROUND: Sperm concentration needed for artificial insemination (AI) to obtain reasonable fertility, taking into consideration genetic value of bull and numerous others components is one of the essential constituents for successful breeding program. AI-doses containing low sperm numbers are increasingly widespread to optimize the benefit of elite bulls, as well as to accommodate an eventual wider application of sex-sorted semen. Viability is reduced when higher dilutions of semen are carried out for production of low sperm doses...
November 2017: Cryo Letters
https://www.readbyqxmd.com/read/29733416/administration-of-recombinant-bovine-somatotropin-prior-to-fixed-time-artificial-insemination-and-the-effects-on-fertility-embryo-and-fetal-size-in-beef-heifers
#11
Nicola Oosthuizen, Pedro L P Fontes, Darren D Henry, Francine M Ciriaco, Carla D Sanford, Luara B Canal, Gentil V de Moraes, Nicolas DiLorenzo, John F Currin, Sherrie Clark, William D Whittier, Vitor R G Mercadante, G Cliff Lamb
Our objectives were to determine the effects of the administration of recombinant bovine somatotropin (bST) at the initiation of a fixed-time AI (TAI) protocol on concentrations of plasma IGF-1, follicle diameter, embryo/fetal size, and pregnancy rates in replacement beef heifers. Four hundred and fourteen Angus-based beef heifers were enrolled in a completely randomized design at 4 locations from January to July of 2016. All heifers were exposed to the 7-d CO-Synch + controlled internal drug release (CIDR) protocol where they received a 100-µg injection of GnRH and a CIDR insert on day -9, 25 mg of PGF2α at CIDR removal on day -2, followed by a 100-µg injection of GnRH and TAI 54 ± 2 h later on day 0...
May 4, 2018: Journal of Animal Science
https://www.readbyqxmd.com/read/29729909/the-relationship-between-serum-anti-m%C3%A3-llerian-hormone-concentrations-and-fertility-and-genome-wide-associations-for-anti-m%C3%A3-llerian-hormone-in-holstein-cows
#12
M Gobikrushanth, D C Purfield, M G Colazo, S T Butler, Z Wang, D J Ambrose
The objectives of this study were to (1) evaluate factors associated with variation in circulating anti-Müllerian hormone (AMH) concentrations, (2) establish an optimum AMH threshold predictive of pregnancy to first artificial insemination (P/AI), (3) examine the relationship between AMH and fertility (P/AI, pregnancy loss between 30 and 60 d after artificial insemination, and pregnancy risk up to 250 d postpartum), and (4) identify quantitative trait loci associated with phenotypic variation of AMH concentrations in dairy cows...
May 2, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29717688/review-semen-handling-time-of-insemination-and-insemination-technique-in-cattle
#13
M G Diskin
In cattle artificial insemination plays not only a vital role in the successful establishment of pregnancy, which is a prerequisite for initiation of the subsequent lactation, but also in accelerating genetic improvement and facilitating the distribution of semen from genetically elite sires. The latter has been greatly facilitated by the ability to successfully cryopreserve semen. The objective of an insemination is to ensure that there is an adequate reservoir of competent, capacitated, motile sperm in the caudal region of the oviductal isthmus, the site of the main sperm reservoir in the cow, at the time of ovulation to ensure fertilisation...
May 2, 2018: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/29717166/comparative-proteomics-reveal-the-association-between-spanx-proteins-and-clinical-outcomes-of-artificial-insemination-with-donor-sperm
#14
X M Wang, Z Xiang, Y Fu, H L Wu, W B Zhu, L Q Fan
Semen analysis is used for diagnosing male infertility and evaluating male fertility for more than a century. However, the semen analysis simply represents the population characteristics of sperm. It is not a comprehensive assessment of the male reproductive potential. In this study, 20 semen samples from human sperm bank with distinctive artificial insemination with donor sperm (AID) clinical outcomes were collected and analyzed using a two-dimensional differential in-gel electrophoresis (2D-DIGE); 45 differentially expressed protein spots were obtained, and 26 proteins were identified...
May 1, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29705068/sperm-chromatin-alterations-in-fertile-and-subfertile-bulls
#15
Elisson Terêncio Souza, Cláudio Vieira Silva, Bruno Augusto Nassif Travençolo, Benner Geraldo Alves, Marcelo Emílio Beletti
Alterations in sperm chromatin have been related with subfertility in several mammals. In this study, chromatin alteration types (Base, Basal half, Central axis, Dispersed, and Whole) were assessed by toluidine blue (TB) staining, 6-diamidino-2-fenilindole (DAPI) and anti-protamine 1 antibody (anti-PR1) labeling in sperm samples of fertile and subfertile bulls. Semen samples were obtained from bulls kept in Artificial Insemination Center (fertile bulls) or from bulls subjected to scrotal insulation (subfertile bulls)...
April 25, 2018: Reproductive Biology
https://www.readbyqxmd.com/read/29688495/supplementing-ca-salts-of-soybean-oil-after-artificial-insemination-increases-pregnancy-success-in-bos-taurus-beef-cows
#16
A P Brandão, R F Cooke, K M Schubach, R S Marques, D W Bohnert, R S Carvalho, N W Dias, C L Timlin, S Clark-Deener, J F Currin, D B Jump, K G Pohler, R L A Cerri, V R G Mercadante
Two experiments investigated the effects of supplementing Ca salts of soybean oil (CSSO) during early gestation on reproductive function and pregnancy rates to artificial insemination (AI) in Bos taurus beef cows. In Exp. 1, 771 suckled, lactating, multiparous Angus cows were divided into 22 groups of approximately 35 cows/group, and timed-inseminated on d 0. After AI, groups were assigned randomly to receive (as-fed basis) 100 g of ground corn + 100 g of soybean meal per cow/d, in addition to: 1) 100 g/cow daily of CSSO (n = 11), or 2) 87 g of prilled saturated fat + 13 g of limestone per cow/d (CON; n = 11)...
April 24, 2018: Journal of Animal Science
https://www.readbyqxmd.com/read/29684811/behavioral-semiochemical-and-androgen-responses-by-male-giant-pandas-to-the-olfactory-sexual-receptivity-cues-of-females
#17
Abbey E Wilson, Darrell L Sparks, Katrina K Knott, Andrew J Kouba, Scott Willard, Ashli Brown
Male giant pandas identify female sexual receptivity through the detection of olfactory cues in estrous urine. However, it is yet unknown which specific days of the female estrous cycle may provoke male sexual-social responses and a physiological readiness to mate. We hypothesized that female urine from specific days of the estrous cycle will be positively associated with specific changes in male behaviors, urinary semiochemical production, and steroidogenic activity. Experimental simultaneous choice trials were conducted in captivity with four male giant pandas during the spring breeding season and during fall...
April 11, 2018: Theriogenology
https://www.readbyqxmd.com/read/29684167/reproduction-in-domestic-ruminants-during-the-past-50-years-discovery-to-application
#18
Michael F Smith, Rodney D Geisert, John J Parrish
The study of reproductive physiology in domestic ruminants has progressed from the whole animal to the molecular level in an amazingly short period of time. The volume of information on this subject is enormous; therefore, we have focused on domestic ruminants, with an emphasis on cattle. To date, artificial insemination (AI) is perhaps the most powerful technique that reproductive physiologists and geneticists have provided the livestock industry for genetic improvement. Early efforts to establish AI as a tool were initiated in Russia around 1899 and since that time major advances in methods of semen collection, evaluation of male fertility, cryopreservation of sperm, sex-sorted semen, and estrous cycle control have occurred...
April 19, 2018: Journal of Animal Science
https://www.readbyqxmd.com/read/29681721/progesterone-hypersensitivity-a-challenge-for-luteal-support
#19
Astha Gupta, Deepak Goenka, Mohan L Goenka
Progesterone hypersensitivity is a rare phenomenon which can occur after both endogenous and exogenous exposures. We present a case of hypersensitivity to various forms and routes of exogenous progesterone. A 27-year-old female presented with primary infertility. Investigations revealed Grade 1 endometriosis and polycystic ovary syndrome. Three cycles of intrauterine insemination were attempted which were unsuccessful and in vitro fertilization was proceeded. Six blastocysts of Grade A were formed and cryopreserved...
January 2018: Journal of Human Reproductive Sciences
https://www.readbyqxmd.com/read/29680455/current-review-of-artificial-insemination-in-dogs
#20
REVIEW
Stuart J Mason
Artificial insemination is the collection of semen from the male and the subsequent insertion of the collected semen into the female. Artificial insemination may be requested for several reasons, including inability to achieve a mating or due to the use of fresh chilled or frozen semen. A good understanding of the cycle of the bitch is imperative for maximizing pregnancy rates, as poor timing of insemination is the most common cause of subfertility in the bitch. Insemination techniques commonly undertaken in the bitch include vaginal insemination, surgical intrauterine insemination, and transcervical insemination...
April 18, 2018: Veterinary Clinics of North America. Small Animal Practice
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