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Artificial insemination

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https://www.readbyqxmd.com/read/28736292/reproductive-features-and-use-of-an-anti-inflammatory-drug-in-estrus-induced-dairy-goats-artificially-inseminated-in-a-standing-position-with-cervix-immobilization
#1
Jeferson Ferreira Fonseca, Gilmar Pereira Alvim, Joanna Maria Gonçalves Souza-Fabjan, Maria Emília Franco Oliveira, Viviane Lopes Brair, Felipe Zandonadi Brandão, Olivardo Facó
This study evaluated reproductive features and role of Flunixin-Meglumine at timed artificial insemination (AI), using a new technique of standing position with cervix immobilization. In Experiment 1, 10 goats (n=5 nulliparous [Null] and 5 pluriparous [Plu]) were evaluated after estrus induction by recorded reproductive parameters to define the ideal time for AI. In Experiment 2, goats were artificially inseminated 51-54h after sponge removal with frozen-thawed semen. At AI, 1mL saline (CONTROL; 18 Null and 14 Plu) or 50mg Flunixin-Meglumine (FLUNIXIN; 15 Null and 18 Plu) was administered i...
July 20, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28734228/effect-of-education-on-ability-of-ai-professionals-and-herd-owner-inseminators-to-detect-cows-not-in-oestrus-and-its-relation-with-progesterone-concentration-on-day-of-re-insemination
#2
K Vartia, J Taponen, J Heikkinen, H Lindeberg
The effect of training background of persons performing artificial insemination (AI) (herd-owner inseminators (OWNER), AI technicians (AI-T), and fertility consultants (FC)) on pregnancy rate and their ability to detect cows not in oestrus were studied. A total of 1584 re-AI occasions on 754 dairy farms were included. Milk samples for progesterone (P4) analysis in all cases were collected, as were data on the herd, previous breeding attempts, oestrous signs, uterine tone, slipperiness of cervix, and co-operation of the cow...
July 13, 2017: Theriogenology
https://www.readbyqxmd.com/read/28722364/-allergy-to-human-seminal-plasma
#3
Laura Moi, Igor Salvadé, Camillo Ribi
Hypersensitivity to human seminal plasma is rare, but probably under-diagnosed. It should be suspected in women with unexplained anaphylaxis, vulvo-vaginitis, pelvic pain and dyspareunia. The diagnosis relies on the clinical presentation, the exclusion of other causes, a positive skin test result to seminal plasma and the detection of IgE directed to allergenic proteins (such as prostate specific antigen). Use of condoms is the best preventive measure. Premedication with antihistamines or anti-inflammatory agents is often unreliable...
April 5, 2017: Revue Médicale Suisse
https://www.readbyqxmd.com/read/28720157/the-influence-of-azaperone-treatment-at-weaning-on-reproductive-performance-of-sows-altering-effects-of-season-and-parity
#4
T Schwarz, J Nowicki, R Tuz, P M Bartlewski
Azaperone treatment can control aggression and decrease stress due to weaning, re-grouping and hierarchical fighting of gilts and sows. However, the effects of this butyrophenone neuroleptic and sedative administered at weaning on pig reproductive function are poorly characterized. In this year-long study, a total of 619 cross-bred sows (Polish Large White×Polish Landrace) kept on a commercial farm received an i.m. injection of azaperone (Stresnil®; 2 mg/kg BW) just before weaning and were artificially inseminated during the ensuing estrus with 3×109 spermatozoa per dose of an inseminate; 1180 sows served as untreated controls...
July 19, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28712062/genome-editing-and-genetic-engineering-in-livestock-for-advancing-agricultural-and-biomedical-applications
#5
Bhanu P Telugu, Ki-Eun Park, Chi-Hun Park
Genetic modification of livestock has a longstanding and successful history, starting with domestication several thousand years ago. Modern animal breeding strategies predominantly based on marker-assisted and genomic selection, artificial insemination, and embryo transfer have led to significant improvement in the performance of domestic animals, and are the basis for regular supply of high quality animal derived food. However, the current strategy of breeding animals over multiple generations to introduce novel traits is not realistic in responding to the unprecedented challenges such as changing climate, pandemic diseases, and feeding an anticipated 3 billion increase in global population in the next three decades...
July 15, 2017: Mammalian Genome: Official Journal of the International Mammalian Genome Society
https://www.readbyqxmd.com/read/28708535/comparison-of-estrus-synchronization-by-controlled-internal-drug-release-device-cidr-and-adhesive-transdermal-progestin-patch-in-postpartum-beef-cows
#6
Jatuporn Kajaysri, Chaiwat Chumchoung, Supphathat Wutthiwitthayaphong, Wanvipa Suthikrai, Praphai Sangkamanee
Estrous synchronization with progesterone based protocols has been essentially used in cattle industry. Although intravaginal devices have been commonly used, this technique may induce vaginitis. This study aimed at examining the efficiency of novel transdermal progestin patch on follicle development and comparing the progestin patch versus CIDR device on estrous synchronization, complication at treated site and pregnancy in beef cattle. In experiment 1, seven beef cows were treated with an adhesive transdermal progestin patch on the ventral surface of the proximal part of the tail for 7 days...
June 6, 2017: Theriogenology
https://www.readbyqxmd.com/read/28708513/interaction-of-extender-composition-and-freezing-method-for-effective-semen-cryopreservation-in-the-north-american-river-otter-lontra-canadensis
#7
Helen L Bateman, William F Swanson
Semen cryopreservation and storage in genome resource banks (GRBs), in combination with artificial insemination (AI), could be invaluable for genetic management and conservation of endangered otter species. For any applied conservation benefit, effective methods for otter sperm processing and cryopreservation first must be established. In this study, our objective was to develop an effective semen cryopreservation method for the North American river otter, evaluating the effect of extender composition (i.e...
June 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28708510/changes-in-localization-and-density-of-cd63-positive-exosome-like-substances-in-the-hen-oviduct-with-artificial-insemination-and-their-effect-on-sperm-viability
#8
A Huang, N Isobe, Y Yoshimura
Avian sperm are stored in the sperm storage tubules (SSTs) of the hen oviduct for a prolonged period. However, the precise mechanisms by which sperm are kept alive in the SSTs are still not fully understood. The aim of this study was to determine whether exosomes are secreted by SST cells and play a role in the survival of sperm. Utero-vaginal junction (UVJ) tissue from approximately 50 wk old White Leghorn hens was collected before (control group) and after intravaginal insemination with seminal plasma (SP group) or semen (AI group)...
June 29, 2017: Theriogenology
https://www.readbyqxmd.com/read/28708499/split-time-artificial-insemination-in-beef-cattle-iii-comparing-fixed-time-artificial-insemination-to-split-time-artificial-insemination-with-delayed-administration-of-gnrh-in-postpartum-cows
#9
B E Bishop, J M Thomas, J M Abel, S E Poock, M R Ellersieck, M F Smith, D J Patterson
This experiment was designed to compare pregnancy rates in postpartum beef cows following split-time (STAI) or fixed-time (FTAI) artificial insemination. Estrus was synchronized for 671 cows at seven locations following administration of the 7-d CO-Synch + CIDR protocol (100 μg GnRH + CIDR insert [1.38 g progesterone] on d 0; 25 mg prostaglandin F2α [PG] at CIDR removal on d 7). Cows were assigned to treatments that were balanced across locations based on age, body condition score, and days postpartum at the time treatments were initiated...
April 29, 2017: Theriogenology
https://www.readbyqxmd.com/read/28701622/effects-on-glycocalyx-structures-of-frozen-thawed-bovine-sperm-induced-by-flow-cytometry-and-artificial-capacitation
#10
Kohei Umezu, Yuuki Hiradate, Takashi Numabe, Kenshiro Hara, Kentaro Tanemura
Sperm sorting by flow cytometry is a useful technology in the bovine industry, but the conception rates after artificial insemination using sex-sorted sperm are lower than when using the un-sorted sperm. In this study, we have investigated the causes for these low conception rates. We have focused on changes caused by flow cytometry to the glycocalyx, which forms the outermost surface of the sperm membrane. We have also evaluated the effects of capacitation on the glycocalyx since capacitation involves a redistribution of the sperm membrane that is vital for successful fertilization and conception...
July 13, 2017: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/28700645/overweight-in-young-males-reduce-fertility-in-rabbit-model
#11
Francisco Marco-Jiménez, José Salvador Vicente
Semen quality has certainly declined over the past few decades, possibly owing to modern lifestyle factors. In this sense, the role of overweight and obesity in the development of subfertility in males has generated a considerable amount of interest in recent years. However, there is no consensus on whether overweight or obesity impaired sperm quality. Thus, based on the ongoing debate about risk factors for subfertility associated with overweight and obesity in men, this study was designed to investigate the effect of overweight on sperm quality parameters and fertility success in randomized controlled trial in a rabbit model...
2017: PloS One
https://www.readbyqxmd.com/read/28699207/supplemental-effect-of-different-levels-of-taurine-in-modena-on-boar-semen-quality-during-liquid-preservation-at-17%C3%A2-c
#12
Hao Li, Xiao-Gang Zhang, Qian Fang, Qi Liu, Ren-Rang Du, Gong-She Yang, Li-Qiang Wang, Jian-Hong Hu
Peroxidation damage induces sublethal injury to boar sperm during the storage process. Taurine has already been demonstrated to protect cells effectively from oxidant-induced injury. This study was aimed to evaluate the effect of different concentrations of taurine (0.5, 1, 5 and 10 mmol/L) in Modena diluent on boar sperm quality during liquid storage at 17°C. Ejaculates from sexually mature Duroc pigs were collected, pooled and preserved in the Modena containing different concentrations of taurine. Sperm motility, plasma membrane integrity, acrosome integrity, total antioxidative capacity (T-AOC) activity and malondialdehyde content (MDA) were examined every 24 h...
July 12, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28695606/orally-administered-chrysin-improves-post-thawed-sperm-quality-and-fertility-of-rooster
#13
M Zhandi, M Ansari, P Roknabadi, A Zare Shahneh, M Sharafi
Chrysin is a bioflavonoid compound found in passion flower, chamomile, propolis and honey at high levels. Post-thawed sperm quality and fertility of Chrysin-fed roosters were assessed in this study. Twenty 40-week-old male broiler breeders were randomly divided into four groups and fed basal diet supplemented with different levels of Chrysin including 0 (Ch-0), 25 (Ch-25), 50 (Ch-50) or 75 (Ch-75) mg/day for 12 consecutive weeks. Semen samples were weekly collected from 6th to 9th week of experiment to evaluate some sperm quality parameters including total and progressive motility, plasma membrane integrity and functionality (in fresh and post-thawed samples) and mitochondrial activity (only in post-thawed samples)...
July 10, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28691353/reproductive-performance-of-immobilized-cryopreserved-bovine-semen-used-for-timed-artificial-insemination
#14
A H Alm-Kristiansen, G Dalen, G Klinkenberg, L Bekk, L T Thorkildsen, K E Waterhouse, E Kommisrud
The SpermVital(®) technology comprises embedding of spermatozoa within an alginate gel to facilitate release of sperm cells over a prolonged period in utero after AI. The aim of this study was to examine whether the survival time of spermatozoa is extended when applying this immobilization technology in combination with cryopreservation. Sperm cell survival (acrosome and plasma membrane integrity) was studied in vitro for 48 hr at physiological temperature. One dose of SpermVital(®) (SV) semen was compared with single doses of Biladyl(®) (B) processed semen as well as double doses of B (B double)...
July 9, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28691349/ovsynch-plus-protocol-improves-ovarian-response-in-anovular-murrah-buffaloes-in-low-breeding-season
#15
R K Sharma, S K Phulia, A Jerome, I Singh
The objective of the study was to evaluate the efficacy of ovarian response and pregnancy rate in anovular buffaloes following Ovsynch and Ovsynch Plus protocols. Buffaloes (n = 55) were divided into two groups: Ovsynch group (n = 26): GnRH (10 μg, GnRH1) on Day 0, PGF2 α (25 mg) on Day 7, GnRH (10 μg, GnRH2) on Day 9; Ovsynch Plus group (n = 29): 500 IU equine chorionic gonadotropin (eCG) 72 hr (day -3) prior to Ovsynch protocol, followed by fixed timed artificial insemination (FTAI) 6 and 24 hr after GnRH2 injection in bot groups...
July 9, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28689480/prevalence-and-risk-factors-of-brucellosis-among-veterinary-health-care-professionals
#16
Rajeswari Shome, Triveni Kalleshamurthy, Padmashree B Shankaranarayana, Prashanth Giribattanvar, Nagarathna Chandrashekar, Nagalingam Mohandoss, Bibek Ranjan Shome, Ashok Kumar, Sukhadeo B Barbuddhe, Habibur Rahman
The study describes prevalence, clinical symptoms and risk factors for brucellosis in personnel engaged in veterinary health care in Karnataka, India. A total of 1050 sera samples were collected from animal handlers, veterinarians, veterinary students, para-veterinarians and persons engaged in artificial insemination of animals. The sera samples were tested for brucellosis by Rose Bengal plate test (RBPT), serum agglutination test (SAT), IgG and IgM indirect ELISA and PCR. Age, sex, clinical symptoms and risk factors were recorded in structured questionnaire...
July 8, 2017: Pathogens and Global Health
https://www.readbyqxmd.com/read/28669062/comparative-efficacy-of-oestrus-synchronization-protocols-in-buffalo-bubalus-bubalis
#17
R Rathore, R K Sharma, S K Phulia, V Mudgal, A Jerome, S P S Ghuman, I Singh
This study was conducted to test the efficacy of gonadotropic hormone (GnRH)-based synchronization protocols (Ovsynch, Heatsynch, and Ovsynch Plus) in buffaloes under field condition. Based on anamnesis and transrectal palpation twice at 10-day interval and serum progesterone (P4) concentration, 150 anoestrous buffaloes and delayed pubertal heifers were selected to induce oestrus using GnRH-based protocols. These selected animals were randomly divided into three groups: group I: Ovsynch (n = 50), group II: Heatsynch (n = 50), and group III: Ovsynch Plus (n = 50) regimen...
July 1, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28668536/high-urea-and-pregnancy-or-conception-in-dairy-cows-a-meta-analysis-to-define-the-appropriate-urea-threshold
#18
D Raboisson, A Albaaj, G Nonne, G Foucras
Dietary proteins play an important role in reproduction, and increased dietary crude proteins, increased degradability of dietary proteins, and elevated blood or milk urea have been associated with decreased conception and pregnancy in many studies. The aim of this work was to provide a meta-analysis on the relationship between high milk or blood urea and pregnancy or conception, with a focus on defining the appropriate urea threshold associated with this issue. The meta-analysis included 61 different models from 21 papers...
June 28, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28668533/resynchronization-of-ovulation-protocols-for-dairy-cows-including-or-not-including-gonadotropin-releasing-hormone-to-induce-a-new-follicular-wave-effects-on-re-insemination-pattern-ovarian-responses-and-pregnancy-outcomes
#19
R Wijma, M L Stangaferro, M Masello, G E Granados, J O Giordano
Our objectives were to evaluate the pattern of re-insemination, ovarian responses, and pregnancy per artificial insemination (P/AI) of cows submitted to different resynchronization of ovulation protocols. The base protocol started at 25 ± 3 d after artificial insemination (AI) and was as follows: GnRH, 7 and 8 d later PGF2α, GnRH 32 h after second PGF2α, and fixed timed AI (TAI) 16 to 18 h after GnRH. At 18 ± 3 d after AI, cows were randomly assigned to the G25 (n = 1,100) or NoG25 (n = 1,098) treatments...
June 28, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28664146/oviductal-transcriptional-profiling-of-a-bovine-fertility-model-by-next-generation-sequencing
#20
Angela Maria Gonella-Diaza, Sónia Cristina da Silva Andrade, Mariana Sponchiado, Guilherme Pugliesi, Fernando Silveira Mesquita, Veerle Van Hoeck, Ricardo de Francisco Strefezzi, Gustavo R Gasparin, Luiz L Coutinho, Mario Binelli
In cattle, the oviduct plays a fundamental role in the reproductive process. Oviductal functions are controlled by the ovarian sex steroids: estradiol and progesterone. Here, we tested the hypothesis that the exposure to contrasting sex steroid milieus differentially impacts the oviductal transcriptional profile. We manipulated growth of the pre-ovulatory follicle to obtain cows that ovulated a larger (LF group) or a smaller (SF group) follicle. The LF group presented greater proestrus/estrus concentrations of estradiol and metaestrus concentrations of progesterone (Gonella-Diaza et al...
September 2017: Genomics Data
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