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https://www.readbyqxmd.com/read/28334050/improving-transcriptome-de-novo-assembly-by-using-a-reference-genome-of-a-related-species-translational-genomics-from-oil-palm-to-coconut
#1
Alix Armero, Luc Baudouin, Stéphanie Bocs, Dominique This
The palms are a family of tropical origin and one of the main constituents of the ecosystems of these regions around the world. The two main species of palm represent different challenges: coconut (Cocos nucifera L.) is a source of multiple goods and services in tropical communities, while oil palm (Elaeis guineensis Jacq) is the main protagonist of the oil market. In this study, we present a workflow that exploits the comparative genomics between a target species (coconut) and a reference species (oil palm) to improve the transcriptomic data, providing a proteome useful to answer functional or evolutionary questions...
2017: PloS One
https://www.readbyqxmd.com/read/28330188/purification-and-characterization-of-a-surfactant-compatible-lipase-from-aspergillus-tamarii-jgif06-exhibiting-energy-efficient-removal-of-oil-stains-from-polycotton-fabric
#2
Arijit Das, Srividya Shivakumar, Sourav Bhattacharya, Sujina Shakya, S S Swathi
An extracellular lipase with 23,666.66 U/ml/min activity was produced by Aspergillus tamarii JGIF06 under submerged fermentation in mineral salt medium containing coconut oil (2.5 % v/v), tryptone (2 % w/v) and ammonium chloride (2 % w/v), with initial pH of 5 ± 0.2, incubated at 25 °C for 7 days on a rotary shaker at 120 rpm. A 7.9-fold increase in lipase-specific activity was recorded after purification by DEAE Sepharose ion exchange and Sephadex G200 column chromatography. The apparent molecular mass of this enzyme was revealed as 50 kDa by sodium dodecyl sulphate polyacrylamide gel electrophoresis...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28301719/a-reconfigured-kennedy-pathway-which-promotes-efficient-accumulation-of-medium-chain-fatty-acids-in-leaf-oils
#3
Kyle B Reynolds, Matthew C Taylor, Darren P Cullerne, Christopher L Blanchard, Craig C Wood, Surinder P Singh, James R Petrie
Medium chain fatty acids (MCFA, C6-14 fatty acids) are an ideal feedstock for biodiesel and broader oleochemicals. In recent decades, several studies have used transgenic engineering to produce MCFA in seeds oils, though these modifications result in unbalance membrane lipid profiles that impair oil yields and agronomic performance. Given the ability to engineer non-seed organs to produce oils, we have previously demonstrated that MCFA profiles can be produced in leaves, but this also results in unbalanced membrane lipid profiles and undesirable chlorosis and cell death...
March 16, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28277823/the-impact-of-virgin-coconut-oil-and-high-oleic-safflower-oil-on-body-composition-lipids-and-inflammatory-markers-in-postmenopausal-women
#4
Margaret Harris, Andrea Hutchins, Lisa Fryda
This randomized crossover study compared the impact of virgin coconut oil (VCO) to safflower oil (SO) on body composition and cardiovascular risk factors. Twelve postmenopausal women (58.8 ± 3.7 year) consumed 30 mL VCO or SO for 28 days, with a 28-day washout. Anthropometrics included body weight and hip and waist circumference. Fat percent for total body, android and gynoid, fat mass, and lean mass were measured using dual-energy X-ray absorptiometry. Women maintained their typical diet recording 28 days of food records during the study...
March 9, 2017: Journal of Medicinal Food
https://www.readbyqxmd.com/read/28244323/reactivity-of-free-malondialdehyde-during-in-vitro-simulated-gastrointestinal-digestion
#5
Angelique Vandemoortele, Pinar Babat, Mariam Yakubu, Bruno De Meulenaer
An aqueous buffer, a saturated glycerol triheptanoate oil, and a Tween 20 stabilized fully hydrogenated coconut oil-in-water emulsion, all spiked with malondialdehyde, were subjected to in vitro digestion. A dynamic equilibrium between malondialdehyde, its aldol self-condensation products, and its hydrolytic cleavage products was observed. This equilibrium depended upon the kind of sample and the temperature at which these samples were preincubated during 24 h. The presence of oil during gastric digestion protected the aldol self-condensation and cleavage products from conversion to malondialdehyde, which occurred in the aqueous acidic gastric chyme...
March 7, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28242932/dietary-fat-composition-food-matrix-and-relative-polarity-modulate-the-micellarization-and-intestinal-uptake-of-carotenoids-from-vegetables-and-fruits
#6
Purna Chandra Mashurabad, Ravindranadh Palika, Yvette Wilda Jyrwa, K Bhaskarachary, Raghu Pullakhandam
Dietary fat increases carotenoid bioavailability by facilitating their transfer to the aqueous micellar fraction during digestion. However, the specific effect of both quantity and type of dietary fat required for optimal carotenoid absorption remained unexplored. In the present study, the effect of amount and type of vegetable oils on carotenoid micellarization from carrot, spinach, drumstick leaves and papaya using in vitro digestion/Caco-2 cell model have been assessed. Although, dietary fat (0.5-10% w/w) significantly increased the micellarization of carotenoids from all the test foods, the extent of increase was determined by the food matrix (papaya > drumstick = spinach > carrot) and polarity of carotenoids (lutein > β-carotene = α-carotene > lycopene)...
February 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28242912/oxidative-stability-thermal-stability-and-acceptability-of-coconut-oil-flavored-with-essential-oils-from-black-pepper-and-ginger
#7
Janu Chandran, N Nayana, N Roshini, P Nisha
The present study investigates the oxidative and thermal stability of flavoured oils developed by incorporating essential oils from black pepper and ginger to coconut oil (CNO) at concentrations of 0.1 and 1.0% (CNOP-0.1, CNOP-1, CNOG-0.1, CNOG-1). The stability of oils were assessed in terms of free fatty acids, peroxide, p-anisidine, conjugated diene and triene values and compared with CNO without any additives and a positive control with synthetic antioxidant TBHQ (CNOT). It was found that the stability of CNOP-1 and CNOG-1 were comparable with CNOT at both study conditions...
January 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28242902/melting-crystallization-and-storage-stability-of-virgin-coconut-oil-and-its-blends-by-differential-scanning-calorimetry-dsc-and-fourier-transform-infrared-spectroscopy-ftir
#8
Yashi Srivastava, Anil Dutt Semwal, Vallayil Appukuttan Sajeevkumar, G K Sharma
The blends were prepared of virgin coconut oil with refined soyabean oil (VCO-RSOY) and refined safflower oil (VCO-RSAFF). Blending with VCO improved the fatty acid composition which increased the shelf stability of 20:80 VCO-RSOY and VCO-RSAFF up to 12 months in different packaging systems such as low density polyethylene, linear low density polyethylene, metalized polyester pouches, polyethylene teteraphthalate, high density polyethylene (HDPE), Amber HDPE bottle. The specific spectral regions of FTIR proved to be very useful for the determination of adulteration as well as for the study of oxidation process...
January 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28215803/polycyclic-aromatic-hydrocarbons-pahs-produced-in-the-combustion-of-fatty-acid-alkyl-esters-from-different-feedstocks-quantification-statistical-analysis-and-mechanisms-of-formation
#9
Alberto Llamas, Ana-María Al-Lal, María-Jesús García-Martínez, Marcelo F Ortega, Juan F Llamas, Magín Lapuerta, Laureano Canoira
Polycyclic Aromatic Hydrocarbons (PAHs) are pollutants of concern due to their carcinogenic and mutagenic activity. Their emissions are mainly related with the combustion or pyrolysis of the organic matter, such as in fossil fuels combustion. It is important to characterize PAHs in the combustions of biofuels due to their increasing importance in the actual energetic setting. There is a lot of research focused in PAHs emission due to the combustion in diesel engines; but only few of them have analyzed the effect of raw material and type of alcohol used in the transesterification process...
May 15, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28215212/dietary-fat-and-physiological-determinants-of-plasma-chylomicron-remnant-homoeostasis-in-normolipidaemic-subjects-insight-into-atherogenic-risk
#10
Deasy Irawati, John C L Mamo, Karin M Slivkoff-Clark, Mario J Soares, Anthony P James
TAG depleted remnants of postprandial chylomicrons are a risk factor for atherosclerosis. Recent studies have demonstrated that in the fasted state, the majority of chylomicrons are small enough for transcytosis to arterial subendothelial space and accelerate atherogenesis. However, the size distribution of chylomicrons in the absorptive state is unclear. This study explored in normolipidaemic subjects the postprandial distribution of the chylomicron marker, apoB-48, in a TAG-rich lipoprotein plasma fraction (Svedberg flotation rate (Sf>400), in partially hydrolysed remnants (Sf 20-400) and in a TAG-deplete fraction (Sf<20), following ingestion of isoenergetic meals with either palm oil (PO), rice bran or coconut oil...
February 20, 2017: British Journal of Nutrition
https://www.readbyqxmd.com/read/28198126/coconut-oil-the-future-of-atopic-dermatitis-treatment
#11
Anand S Desai
No abstract text is available yet for this article.
February 15, 2017: Dermatologic Therapy
https://www.readbyqxmd.com/read/28188639/effect-of-unconventional-oilseeds-safflower-poppy-hemp-camelina-on-in-vitro-ruminal-methane-production-and-fermentation
#12
Shaopu Wang, Michael Kreuzer, Ueli Braun, Angela Schwarm
BACKGROUND: Dietary supplementation with oilseeds can reduce methane emission in ruminants, but only a few common seeds have been tested so far. This study tested safflower (Carthamus tinctorius), poppy (Papaver somniferum), hemp (Cannabis sativa), and camelina (Camelina sativa) seeds in vitro using coconut (Cocos nucifera) oil and linseed (Linum usitatissimum) as positive controls. RESULTS: All the tested oilseeds suppressed methane yield (ml g(-1) dry matter, up to 21%) compared to the non-supplemented control when provided at 70 g oil kg(-1) dry matter, and they were as effective as coconut oil...
February 11, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28179911/characterization-and-ectopic-expression-of-cowri1-an-ap2-erebp-domain-containing-transcription-factor-from-coconut-cocos-nucifera-l-endosperm-changes-the-seeds-oil-content-in-transgenic-arabidopsis-thaliana-and-rice-oryza-sativa-l
#13
RuHao Sun, Rongjian Ye, Lingchao Gao, Lin Zhang, Rui Wang, Ting Mao, Yusheng Zheng, Dongdong Li, Yongjun Lin
Coconut (Cocos nucifera L.) is a key tropical crop and a member of the monocotyledonous family Arecaceae (Palmaceae). Few genes and related metabolic processes involved in coconut endosperm development have been investigated. In this study, a new member of the WRI1 gene family was isolated from coconut endosperm and was named CoWRI1. Its transcriptional activities and interactions with the acetyl-CoA carboxylase (BCCP2) promoter of CoWRI1 were confirmed by the yeast two-hybrid and yeast one-hybrid approaches, respectively...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28161866/utilization-of-agro-industry-residue-for-rhamnolipid-production-by-p-aeruginosa-amb-as7-and-its-application-in-chromium-removal
#14
Mariaamalraj Samykannu, Anant Achary
Coconut oil sludge and oil cake was utilized as carbon source for biosurfactant production by Pseudomonas aeruginosa AMB AS7. The results of optimization study revealed that 1.5% (w/v) of coconut oil cake, 2% (w/v) of coconut oil sludge, pH 7.2, 37 °C, and 120 rpm were the optimum conditions for biosurfactant production. The yield coefficient of biosurfactant on biomass (Y P/X ) was 1.29 g/g. Besides, the results indicated that aeration of 0.5 vvm and agitation of 450 rpm in bioreactor resulted in high volumetric productivity of biosurfactant (r p ) and specific product formation rate (q p ) of 0...
February 4, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28130913/selective-pre-column-derivatization-of-fatty-acids-with-the-fluorescent-tag-6-aminoquinoline-and-their-determination-in-some-food-samples-by-reversed-phase-chromatography
#15
Murthy Jonnada, Guadalupe Davila El Rassi, Ziad El Rassi
Fatty acids (FAs) have been selectively derivatized with a fluorescent tag, 6-aminoquinoline (6AQ), which yielded fluorescent FA-6AQ derivatives that have excitation (λexc = 270 nm) and emission (λemi = 495 nm) wavelengths that are farther apart. This precolumn derivatization is characterized by its simplicity occurring at room temperature between the carboxylic acid group of the FA and the amino group of 6AQ in the presence of a non-aqueous soluble carbodiimide coupling agent such as the N,N´-dicyclohexylcarbodiimide...
January 28, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28129764/maternal-intake-of-dietary-virgin-coconut-oil-modifies-essential-fatty-acids-and-causes-low-body-weight-and-spiky-fur-in-mice
#16
Renuka Gunasekaran, Mohammed Rafid Shaker, Siti Waheeda Mohd-Zin, Aminah Abdullah, Azlina Ahmad-Annuar, Noraishah Mydin Abdul-Aziz
BACKGROUND: Coconut oil is commonly used as herbal medicine worldwide. There is limited information regarding its effects on the developing embryo and infant growth. METHODS: We investigated the effect of virgin coconut oil post-natally and until 6 weeks old in mice (age of maturity). Females were fed with either standard, virgin olive oil or virgin coconut oil diets 1 month prior to copulation, during gestation and continued until weaning of pups. Subsequently, groups of pups borne of the respective diets were continuously fed the same diet as its mother from weaning until 6 weeks old...
January 28, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28126466/coconut-oil-protects-cortical-neurons-from-amyloid-beta-toxicity-by-enhancing-signaling-of-cell-survival-pathways
#17
F Nafar, J P Clarke, K M Mearow
Alzheimer's disease is a progressive neurodegenerative disease that has links with other conditions that can often be modified by dietary and life-style interventions. In particular, coconut oil has received attention as having potentially having benefits in lessening the cognitive deficits associated with Alzheimer's disease. In a recent report, we showed that neuron survival in cultures co-treated with coconut oil and Aβ was rescued compared to cultures exposed only to Aβ. Here we investigated treatment with Aβ for 1, 6 or 24 h followed by addition of coconut oil for a further 24 h, or treatment with coconut oil for 24 h followed by Aβ exposure for various periods...
May 2017: Neurochemistry International
https://www.readbyqxmd.com/read/28126345/regulation-of-plasma-glucose-and-sulfate-excretion-in-pacific-hagfish-eptatretus-stoutii-is-not-mediated-by-11-deoxycortisol
#18
Alexander M Clifford, Nicolas R Bury, Aaron G Schultz, James D Ede, Brendan L Goss, Greg G Goss
The goal of this study was to identify whether Pacific hagfish (Eptatretus stoutii) possess glucocorticoid and mineralocorticoid responses and to examine the potential role(s) of four key steroids in these responses. Pacific hagfish were injected with varying amounts of cortisol, corticosterone or 11-deoxycorticosterone (DOC) using coconut oil implants and plasma glucose and gill total-ATPase activity were monitored as indices of glucocorticoid and mineralocorticoid responses. Furthermore, we also monitored plasma glucose and 11-deoxycortisol (11-DOC) levels following exhaustive stress (30 min of agitation) or following repeated infusion with SO4(2-)...
January 23, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28124592/acyclovir-solid-lipid-nanoparticles-for-skin-drug-delivery-fabrication-characterization-and-in-vitro-study
#19
Maria John Newton, Kaur Bhupinder
OBJECTIVE: The fractionated coconut oil based solid lipid nanoparticles (SLNs) of Acyclovir (ACV) were fabricated in two batches by using Glyceryl mono stearate (GMS) and Lipoid S75 (Fat free soybean phospholipids with 70% phosphatidylcholine- Lipoid GmbH, Germany). The research was focused on developing ACV-SLN by using high pressure hot-homogenization technique. The ingredients were used in different concentrations and ratios to identify the best formulation design. METHODS: The GMS with Fractionated coconut oil and Lipoid S 75 with Fractionated coconut oil were used in various concentrations in formulation design to assess the impact on the fabrication and evaluation of SLNs...
January 17, 2017: Recent Patents on Drug Delivery & Formulation
https://www.readbyqxmd.com/read/28118770/enhanced-memory-in-wistar-rats-by-virgin-coconut-oil-is-associated-with-increased-antioxidative-cholinergic-activities-and-reduced-oxidative-stress
#20
Nur Syafiqah Rahim, Siong Meng Lim, Vasudevan Mani, Abu Bakar Abdul Majeed, Kalavathy Ramasamy
CONTEXT: Virgin coconut oil (VCO) has been reported to possess antioxidative, anti-inflammatory and anti-stress properties. OBJECTIVE: Capitalizing on these therapeutic effects, this study investigated for the first time the potential of VCO on memory improvement in vivo. MATERIALS AND METHODS: Thirty male Wistar rats (7-8 weeks old) were randomly assigned to five groups (n = six per group). Treatment groups were administered with 1, 5 and 10 g/kg VCO for 31 days by oral gavages...
December 2017: Pharmaceutical Biology
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