Read by QxMD icon Read


C Sartori, V Perreten, I Ivanovic, M C Härdi-Landerer, H U Graber
In Switzerland, sanitation programs of dairy herds infected with the contagious mastitis pathogen Staphylococcus aureus genotype B (GTB) have been established for several years. In recent years, Streptococcus uberis and non-aureus staphylococci have emerged as the bacteria most frequently isolated from bovine milk samples. The latter cause subclinical mastitis, and some species are more persistent or pathogenic than others. The present study aimed to investigate the developments in the intramammary colonization spectrum of 5 dairy herds undergoing a sanitation program for Staph...
June 13, 2018: Journal of Dairy Science
Yue Song, Tingting Chai, Zhiqiang Yin, Xining Zhang, Wei Zhang, Yongzhong Qian, Jing Qiu
Ibuprofen (IBU), as a commonly used non-steroidal anti-inflammatory drug (NSAID) and pharmaceutical and personal care product (PPCP), is frequently prescribed by doctors to relieve pain. It is widely released into environmental water and soil in the form of chiral enantiomers by the urination and defecation of humans or animals and by sewage discharge from wastewater treatment plants. This study focused on the alteration of metabolism in the adult zebrafish (Danio rerio) brain after exposure to R-(-)-/S-(+)-/rac-IBU at 5 μg L-1 for 28 days...
June 9, 2018: Environmental Pollution
Wei Lei, Jianan Ni, Xueting Xia, Min Jiang, Gang Bai
Coronary heart disease is a vital cause of morbidity and mortality worldwide, and calcium channel blockers (CCBs) are important drugs that can be used to treat cardiovascular diseases. Suxiao Jiuxin Pill (SX), a traditional Chinese medicine, is widely used as an emergency drug for coronary heart disease therapy. However, understanding its potential mechanism in intracellular calcium concentration ([Ca2+ ]i ) modulation remains a challenge. To identify the active pharmacological ingredients (APIs) and reveal a novel combination therapy for ameliorating cardiovascular diseases, the ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF MS) combined with a dual-luciferase reporter [Ca2+ ]i assay system was applied...
June 8, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Paul K R Dasari, Judson P Jones, Michael E Casey, Yuanyuan Liang, Vasken Dilsizian, Mark F Smith
BACKGROUND: The effect of time-of-flight (TOF) and point spread function (PSF) modeling in image reconstruction has not been well studied for cardiac PET. This study assesses their separate and combined influence on 82 Rb myocardial perfusion imaging in obese patients. METHODS: Thirty-six obese patients underwent rest-stress 82 Rb cardiac PET. Images were reconstructed with and without TOF and PSF modeling. Perfusion was quantitatively compared using the AHA 17-segment model for patients grouped by BMI, cross-sectional body area in the scanner field of view, gender, and left ventricular myocardial volume...
June 15, 2018: Journal of Nuclear Cardiology: Official Publication of the American Society of Nuclear Cardiology
Krzysztof Kamil Wojtanowski, Tomasz Mroczek
The high performance liquid chromatography (HPLC) coupled to electrospray ionization (ESI) octopole orthogonal acceleration time-of-flight (TOF) mass spectrometry (MS) was developed to separate secondary metabolites belonging to different groups of compounds with a wide range of polarity, i.e. phenolic acids, flavonoids, sesquiterpenes and others present in minute amounts, in a single run. Moreover, application of ESI-TOF-MS with a simple in source type of fragmentation and high mass accuracy measurements (around 2 ppm) in both positive and negative ionization modes with different and optimized fragmentor voltages, enabled the identification of each single compound based on proposed fragmentation patterns...
October 31, 2018: Analytica Chimica Acta
Michiro Muraki, Kiyonori Hirota
OBJECTIVE: In this study, we aimed to identify the structural components and to clarify the biological activity in the site-specific conjugates of human Fas ligand extracellular domain (hFasLECD) with either fluorescein moiety (FL) or chicken egg-white avidin (Avi). The conjugates were characterized by molecular-weight measurement using MALDI-TOF mass-spectrometric analysis and by cell-death inducing activity measurement against a human colorectal cancer cell line, HT-29, using MTT cell-viability assay...
June 15, 2018: BMC Research Notes
Susanne Neupert, Debora Fusca, Peter Kloppenburg, Reinhard Predel
Single cell mass spectrometry has become an established technique to study specific molecular properties such as the neuropeptide complement of identified neurons. Here, we describe a strategy to characterize, by MALDI-TOF mass spectrometry, neurochemical properties of neurons that were identified by their electrophysiological and neuroanatomical properties. The workflow for the first time combined perforated patch clamp recordings with dye loading by electroporation for electrophysiological and neuroanatomical characterization as well as chemical profiling of somata by MALDI-TOF mass spectrometry with subsequent immunocytochemistry...
June 15, 2018: ACS Chemical Neuroscience
A C M Veloo, H Jean-Pierre, U S Justesen, T Morris, E Urban, I Wybo, M Kostrzewa, A W Friedrich, On Behalf Of The Enria Workgroup
This data in brief article presents the data obtained during the validation of the optimized Biotyper Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) database. The validation was performed by the different expertise laboratories, collaborating within the European Network for the Rapid Identification of Anaerobes (ENRIA) project, using 6309 human clinical anaerobic bacterial strains. Different databases were compared with each other; the db 5989 database (V5 database); the V5 database complimented with Main Spectral Profiles (MSPs) of ENRIA strains added to the next update of the database; and the V5 database complimented with the MSPs of all anaerobic clinical isolates collected within the ENRIA project...
June 2018: Data in Brief
Holger Husi, Alisdair MacDonald, Richard J E Skipworth, Janice Miller, Andrew Cronshaw, Kenneth C H Fearon, James A Ross
Myosteatosis, the infiltration of fat in skeletal muscle, is associated with lower skeletal muscle density (SMD) as detected by computed tomography (CT). It increases with aging and obesity and is thought to play a role in the aetiology of insulin resistance and type II diabetes. The clinical significance of myosteatosis in cancer cachexia, however, remains to be determined. Along with demonstrable subcutaneous and visceral lipolysis, myosteatosis may also be a key component of the syndrome. We aimed to investigate the use of human urine as a non-invasive way to screen for molecular biomarkers of myosteatosis/reduced SMD using SELDI-TOF mass spectrometry...
June 2018: Biomedical Reports
Holger Husi, Alisdair MacDonald, Richard J E Skipworth, Janice Miller, Andrew Cronshaw, Carolyn Greig, Kenneth C H Fearon, James A Ross
Dynapenia is defined as the age-related loss of muscle strength, and plays a significant role in the loss of physical function and increased risk of disability among older individuals. The need for an early diagnosis supports the search for a biomarker that reflects muscle 'weakening'. This has previously proven difficult due to patient heterogeneity at presentation and lack of understanding of the underlying molecular mechanisms. The aim of the present study was to identify potential urinary biomarkers of dynapenia in patients undergoing potentially curative surgery for upper gastrointestinal cancer...
June 2018: Biomedical Reports
Matthew I Jones, Shakeel A Qureshi
Surgical repair of tetralogy of Fallot (ToF) in childhood is associated with generally good outcomes, and almost all children can be expected to survive until adulthood. However, significant pulmonary regurgitation leading to progressive right ventricular dilatation is common in teenagers or young adults because of the nature of the surgical intervention. In patients whose repair included placement of a right ventricle to pulmonary artery conduit, it has been possible to place a stented valve within the conduit to treat this...
2018: F1000Research
Chenyu Jiang, Baokai Ma, Shuang Song, Oi-Ming Lai, Ling-Zhi Cheong
Phospholipids composition in the milk fat globule membrane (MFGM) fluctuates during entire lactation period in order to suit the growing needs of newborn infants. Present study elucidated and relatively quantified phospholipids molecular species extracted from human milk (HM), mature human milk (MHM) and infant formulas (with or without MFGM supplementation) using HILIC-ESI-IT-TOF-MS system. Principal component analysis was used to clarify the differences between phospholipids composition in HM, MHM and infant formulas...
June 14, 2018: Journal of Agricultural and Food Chemistry
Norman Atkins, Shifang Ren, Nathan G Hatcher, Penny W Burgoon, Jennifer W Mitchell, Jonathan V Sweedler, Martha U Gillette
Daily oscillations of brain and body states are under complex temporal modulation by environmental light and the hypothalamic suprachiasmatic nucleus (SCN), the master circadian clock. To better understand mediators of differential temporal modulation, we characterize neuropeptide releasate profiles by non-selective capture of secreted neuropeptides in an optic nerve-horizontal SCN brain slice model. Releasates are collected following electrophysiological stimulation of the optic nerve/retinohypothalamic tract under conditions that alter the phase of SCN activity state...
June 14, 2018: ACS Chemical Neuroscience
Selina Traxler, Ann-Christin Bischoff, Phillip Trefz, Jochen Schubert, Wolfram Miekisch
Volatile organic compound (VOC) profiles emitted in trace concentrations from bacteria or cells has gained increasing importance over the last decades. Analysis of VOCs in the headspace does not interfere with in vitro systems and, therefore, offers new options for noninvasive monitoring of cultures. Currently there is not any available standardized in-vitro sampling system which considers effects of dilution and contamination onto ppbV to pptV VOC concentrations during. In this study a new in-vitro system for online and offline headspace measurement of biological cultures was designed...
June 14, 2018: Journal of Breath Research
Cristina Cruz, Teresa Pinho, Carla Sousa, Cláudia Camila Dias, José Silva Cardoso, Maria Júlia Maciel
BACKGROUND: Late after tetralogy of Fallot (TOF) repair some patients exhibit aortic dilatation and stiffness. Noninvasive assessment of aortic stiffness could contribute to understand this aortopathy and may be important in risk stratification for major aortic event. METHODS: We included prospectively 82 adults after TOF repair and 41 age- and sex-matched normal controls. Aortic diameters were measured by two-dimensional transthoracic echocardiography and the aortic z-score was estimated...
June 13, 2018: Echocardiography
Rubashiny Veeramohan, Kamalrul Azlan Azizan, Wan Mohd Aizat, Hoe-Han Goh, Sharif Mahsufi Mansor, Nur Sabrina Mohd Yusof, Syarul Nataqain Baharum, Chyan Leong Ng
Mitragyna speciosa is a psychoactive plant known as "ketum" in Malaysia and "kratom" in Thailand. This plant is distinctly known to produce two important alkaloids, namely mitragynine (MG) and 7-hydroxymitragynine (7-OH-MG) that can bind to opioid receptors [1]. MG was reported to exhibit antidepressant properties in animal studies [2]. These compounds were also proposed to have the potential to replace opioid analgesics with much lower risks of side effects [3]. To date, there are only over 40 metabolites identified in M...
June 2018: Data in Brief
Jerome Vialaret, Pierre-Olivier Schmit, Sylvain Lehmann, Audrey Gabelle, Jason Wood, Marshall Bern, Rainer Paape, Detlev Suckau, Gary Kruppa, Christophe Hirtz
Top-Down approaches have an extremely high biological relevance, especially when it comes to biomarker discovery, but the necessary pre-fractionation constraints are not easily compatible with the robustness requirements and the size of clinical sample cohorts. We have demonstrated that intact protein profiling studies could be run on UHR-Q-ToF with limited pre-fractionation (Schmit et al., 2017) [1]. The dataset presented herein is an extension of this research. Proteoforms known to play a role in the pathophysiology process of Alzheimer's disease were identified as candidate biomarkers...
June 2018: Data in Brief
Paola Rey-Suárez, Cristian Acosta, Uday Torres, Mónica Saldarriaga-Córdoba, Bruno Lomonte, Vitelbina Núñez
L-amino acid oxidases (LAAOs) are ubiquitous enzymes in nature. Bioactivities described for these enzymes include apoptosis induction, edema formation, induction or inhibition of platelet aggregation, as well as antiviral, antiparasite, and antibacterial actions. With over 80 species, Micrurus snakes are the representatives of the Elapidae family in the New World. Although LAAOs in Micrurus venoms have been predicted by venom gland transcriptomic studies and detected in proteomic studies, no enzymes of this kind have been previously purified from their venoms...
2018: PeerJ
Wenxiao Jiao, Xiangxin Li, Handong Zhao, Jiankang Cao, Weibo Jiang
Thaumatin-like protein from banana (designated BanTLP) has been purified by employing a simple protocol consisting of diethylaminoethyl Sephadex (DEAE⁻Sephadex) chromatography, gel filtration on Sephadex G50, and reversed-phase chromatography. The purified protein was identified by MALDI-TOF mass spectrometry, with an estimated molecular weight of 22.1 kDa. BanTLP effectively inhibited in vitro spore germination of Penicillium expansum , one of the main postharvest pathogens in fruits. This study further investigated the antifungal properties and underlying mechanisms of BanTLP against P...
June 14, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
M Zubair Israr, Liam M Heaney, Leong L Ng, Toru Suzuki
BACKGROUND: B-type natriuretic peptide (BNP) is known to be a risk marker following acute myocardial infarction (MI). More recently, truncated molecular forms of the BNP molecule have been identified, with the association of these forms and outcome in acute MI not known. The present study investigated their use as risk stratifying biomarkers of this condition. METHODS: BNP molecular forms (BNP 5-32, BNP 4-32 and BNP 3-32) were measured in plasma from 1078 acute MI patients using immunocapture followed by MALDI-ToF-mass spectrometry...
June 2018: American Heart Journal
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"