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https://www.readbyqxmd.com/read/28634118/identification-of-chickpea-seed-proteins-resistant-to-simulated-in-vitro-human-digestion
#1
I C Ribeiro, C C Leclercq, N Simões, A Toureiro, I Duarte, J B Freire, M M Chaves, J Renaut, C Pinheiro
Proteins and peptides able to resist gastrointestinal digestion and reach the intestinal mucosa have the potential to influence human health. Chickpea (Cicer arietinum L.) seed proteins are able to resist cooking (86.9% total protein) and/or in vitro simulated human digestion (15.9% total protein resists soaking, cooking and digestion with pepsin and pancreatin). To identify and characterize proteins resisting digestion we made use of different MS methodologies. The efficiency of several proteases (trypsin, AspN, chymotrypsin and LysC) was tested, and two technologies were employed (MALDI-TOF-TOF and LC-nESI-MS/MS)...
June 17, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28633270/surgical-correction-for-a-neonate-with-ebstein-s-anomaly-associated-with-tetralogy-of-fallot
#2
Ling-Yi Wei, Jin-Chung Shih, En-Ting Wu, Shyh-Jye Chen, Yih-Sharng Chen, Shu-Chien Huang
Ebstein's anomaly in combination with tetralogy of Fallot (TOF) is rare. We report a male infant with these combined anomalies who presented with severe cyanosis, heart failure, and respiratory distress after birth. Biventricular repair was successfully performed with 1-stage correction of his ventricular septal defect (VSD), right ventricular outflow tract (RVOT) obstruction, and tricuspid regurgitation. The downward displaced tricuspid valve was also restored to the normal annulus position. The infant recovered well...
July 2017: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/28633269/stenting-and-reimplanting-disconnected-pulmonary-artery-in-tetralogy-of-fallot
#3
Harikrishnan K N Kurup, Giedrius Baliulis, Marcus P Haw, Joseph J Vettukattil
Tetralogy of Fallot with absent pulmonary valve syndrome (TOF/APV) is a rare congenital malformation. Although pulmonary artery (PA) anomalies have been observed in TOF, its association with disconnected PA is extremely rare. We report successful stenting of the disconnected left PA in a 3-year-old boy with TOF/APV followed by surgical reimplantation. The significance of this transcatheter intervention for guidance during surgery and the importance of visualizing a ductal stump on angiography as an indicator of disconnected PA are discussed...
July 2017: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/28632391/transfer-hydrogenation-of-carbonyl-derivatives-catalyzed-by-an-inexpensive-phosphine-free-manganese-precatalyst
#4
Antoine Bruneau-Voisine, Ding Wang, Vincent Dorcet, Thierry Roisnel, Christophe Darcel, Jean-Baptiste Sortais
A very simple and inexpensive catalytic system based on abundant manganese as transition metal and on an inexpensive phosphine-free bidendate ligand, 2-(aminomethyl)pyridine, has been developed for the reduction of a large variety of carbonyl derivatives with 2-propanol as hydrogen donor. Remarkably, the reaction proceeds at room temperature with low catalyst loading (down to 0.1 mol %) and exhibits a good tolerance toward functional groups. High TON (2000) and TOF (3600 h(-1)) were obtained.
June 20, 2017: Organic Letters
https://www.readbyqxmd.com/read/28631328/phenotypic-characterisation-of-coagulase-negative-staphylococci-isolated-from-blood-cultures-in-newborn-infants-with-a-special-focus-on-staphylococcus-capitis
#5
Gustaf Ehlersson, Bengt Hellmark, Olov Svartström, Bianca Stenmark, Bo Söderquist
AIM: This Swedish study determined which species of coagulase-negative staphylococci (CoNS) were found in neonatal blood cultures and whether they included Staphylococcus capitis clones with decreased susceptibility to vancomycin. METHODS: CoNS isolates (n=332) from neonatal blood cultures collected at Örebro University Hospital during 1987-2014 were identified to species level with matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS)...
June 20, 2017: Acta Paediatrica
https://www.readbyqxmd.com/read/28631300/a-bacterial-chloroform-reductive-dehalogenase-purification-and-biochemical-characterization
#6
Bat-Erdene Jugder, Susanne Bohl, Helene Lebhar, Robert D Healey, Mike Manefield, Christopher P Marquis, Matthew Lee
We report herein the purification of a chloroform (CF)-reducing enzyme, TmrA, from the membrane fraction of a strict anaerobe Dehalobacter sp. strain UNSWDHB to apparent homogeneity with an approximate 23-fold increase in relative purity compared to crude lysate. The membrane fraction obtained by ultracentrifugation was solubilized in Triton X-100 in the presence of glycerol, followed by purification by anion exchange chromatography. The molecular mass of the purified TmrA was determined to be 44.5 kDa by SDS-PAGE and MALDI-TOF/TOF...
June 20, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28631207/qrs-width-as-a-predictor-of-right-ventricular-remodeling-after-percutaneous-pulmonary-valve-implantation
#7
C Paech, I Dähnert, F T Riede, R Wagner, T Kister, K Nieschke, F Wagner, R A Gebauer
Recent data showed a right ventricular dyssynchrony in patients with tetralogy of Fallot (TOF). Percutaneous pulmonary valve implantation (PPVI) has become an important procedure to treat a pulmonary stenosis and/or regurgitation of the right ventricular outflow tract in these patients. Despite providing good results, there is still a considerable number of nonresponders to PPVI. The authors speculated that electrical dysfunction of the right ventricle plays an underestimated role in the outcome of patients after PPVI...
June 19, 2017: Pediatric Cardiology
https://www.readbyqxmd.com/read/28631113/fast-determination-of-ingredients-in-solid-pharmaceuticals-by-microwave-enhanced-in-source-decay-of-microwave-plasma-torch-mass-spectrometry
#8
Rui Su, Xinchen Wang, Changming Hou, Meiling Yang, Keke Huang, Huanwen Chen
Rapid qualitative and quantitative analysis of solid samples (e.g., pharmaceutical preparations) by using a small and low-resolution mass spectrometer without MS/MS function is still a challenge in ambient pressure ionization mass spectrometric analysis. Herein, a practically efficient method termed microwave-enhanced in-source decay (MEISD) using microwave plasma torch desorption ionization coupled with time-of-flight mass spectrometry (MPTDI-TOF MS) was developed for fast analysis of pharmaceutical tablets using a miniature TOF mass spectrometer without tandem mass function...
June 19, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28630686/fecal-carriage-of-extended-spectrum-%C3%AE-lactamase-and-carbapenemase-producing-enterobacteriaceae-in-egyptian-patients-with-community-onset-gastrointestinal-complaints-a-hospital-based-cross-sectional-study
#9
H M Abdallah, N Alnaiemi, E A Reuland, B B Wintermans, A Koek, A M Abdelwahab, A Samy, K W Abdelsalam, C M J E Vandenbroucke-Grauls
OBJECTIVES: The aim of this study was to determine the prevalence of extended-spectrum β-lactamase (ESBL) and carbapenemase production among Enterobacteriaceae isolated from ambulatory patients with gastrointestinal complaints admitted to El-Ahrar General Hospital, Zagazig, Egypt in the period between January 2013 and May 2013. METHODS: One hundred and thirteen Enterobacteriaceae isolates were recovered from 100 consecutive Egyptian patients with community-onset gastrointestinal complaints...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/28630187/cumulative-effect-of-an-antimicrobial-stewardship-and-rapid-diagnostic-testing-bundle-on-early-streamlining-of-antimicrobial-therapy-in-gram-negative-bloodstream-infections
#10
P B Bookstaver, E B Nimmich, T J Smith, J A Justo, J Kohn, K L Hammer, C Troficanto, H A Albrecht, M N Al-Hasan
OBJECTIVES: The use of rapid diagnostic tests (RDT) enhances antimicrobial stewardship programs (ASP) interventions in optimization of antimicrobial therapy. This quasi-experimental cohort study evaluates the combined impact of an ASP/RDT bundle on appropriateness of empirical antimicrobial therapy (EAT) and time to de-escalation of broad-spectrum agents (BSAA) in gram-negative bloodstream infections (GNBSI). METHODS: ASP/RDT bundle consisted of a system-wide GNBSI treatment guidelines, prospective stewardship monitoring, and sequential introduction of two RDTs, Matrix Assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF) and FilmArray® Blood Culture Identification (BCID) panel...
June 19, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28630069/degradation-of-incretins-and-modulation-of-blood-glucose-levels-by-periodontopathic-bacterial-dipeptidyl-peptidase-4
#11
Yuko Ohara-Nemoto, Manami Nakasato, Yu Shimoyama, Tomomi T Baba, Takeshi Kobayakawa, Toshio Ono, Takashi Yaegashi, Shigenobu Kimura, Takayuki K Nemoto
Severe periodontitis is known to aggravate diabetes mellitus, though molecular events related to that link have not been fully elucidated. Porphyromonas gingivalis, a major pathogen of periodontitis, expresses dipeptidyl peptidase 4 (DPP4), which is involved in regulation of blood glucose levels by cleaving incretins in humans. We examined the enzymatic characteristics of DPP4 from P. gingivalis as well as two other periodontopathic bacteria, Tannerella forsythia and Prevotella intermedia, and determined whether it is capable of regulating blood glucose levels...
June 19, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28629818/corydalis-hendersonii-hemsl-protects-against-myocardial-injury-by-attenuating-inflammation-and-fibrosis-via-nf-%C3%AE%C2%BAb-and-jak2-stat3-signaling-pathways
#12
Ruifeng Bai, Xu Yin, Xiao Feng, Yuan Cao, Yan Wu, Zhixiang Zhu, Chun Li, Pengfei Tu, Xingyun Chai
ETHNOPHARMACOLOGICAL RELEVANCE: Corydalis hendersonii Hemsl. (CH) with heat clearing and detoxifying effects are well described in Tibetan folk medicine. It has been used for centuries in China largely for the treatment of high altitude polycythemia, a pathophysiological condition referred to "plethora" in Tibetan medicine, hypertension, hepatitis, edema, gastritis, and other infectious diseases. AIM OF THE STUDY: To investigate the cardioprotective effects of Corydalis hendersonii extract in an ICR mouse model of myocardial ischemic injury...
June 16, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28629229/colistin-resistance-associated-with-outer-membrane-protein-change-in%C3%A2-klebsiella-pneumoniae-and-enterobacter-asburiae
#13
Béla Kádár, Béla Kocsis, Ákos Tóth, Katalin Kristóf, Péter Felső, Béla Kocsis, Katalin Böddi, Dóra Szabó
In this study, outer membrane proteins (OMPs) of colistin-resistant Klebsiella pneumoniae and Enterobacter asburiae were analyzed. One colistin-susceptible and three colistin-resistant K. pneumoniae sequence type 258 strains as well as one colistin-susceptible E. asburiae and its colistin-heteroresistant counterpart strain were involved in the study. OMP analysis of each strain was performed by microchip method. Matrix-assisted laser desorption ionization time of flight/mass spectrometry (MALDI-TOF/MS) investigation was carried out after separation of OMPs by two-dimensional gel electrophoresis and in-gel digestion...
June 20, 2017: Acta Microbiologica et Immunologica Hungarica
https://www.readbyqxmd.com/read/28628752/high-prevalence-of-resistance-to-fluoroquinolones-and-tetracycline-campylobacter-spp-isolated-from-poultry-in-poland
#14
Anna Woźniak-Biel, Gabriela Bugla-Płoskońska, Alicja Kielsznia, Kamila Korzekwa, Anna Tobiasz, Agnieszka Korzeniowska-Kowal, Alina Wieliczko
Campylobacter spp. is a major cause of foodborne diseases in humans, particularly when transmitted by the handling or consumption of undercooked poultry meat. Most Campylobacter infections are self-limiting, but antimicrobial treatment (e.g., fluoroquinolones and macrolides) is necessary in severe or prolonged cases. The indiscriminate use of these drugs, both in clinical medicine and animal production, has a major impact on public health. The aim of the present study was to identify Campylobacter strains, isolated from turkey and broilers, using both PCR and the matrix-assisted laser desorption-ionization time-of-flight (MALDI-TOF) methods to reveal the accuracy of identification, as well to evaluate the antimicrobial and genetic resistance of the investigated strains...
June 19, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28627098/effect-of-concomitant-birth-defects-and-genetic-anomalies-on-infant-mortality-in-tetralogy-of-fallot
#15
Eric G Jernigan, Paula D Strassle, Rebecca C Stebbins, Robert E Meyer, Jennifer S Nelson
BACKGROUND: A substantial proportion of infants born with tetralogy of Fallot (TOF) die in infancy. A better understanding of the heterogeneity associated with TOF, including extracardiac malformations and chromosomal anomalies is vital to stratifying risk and optimizing outcomes during infancy. METHODS: Using the North Carolina Birth Defects Monitoring Program, infants diagnosed with TOF and born between 2003 and 2012 were included. Kaplan-Meier survival curves were used to estimate cumulative 1-year mortality, stratified by the presence of concomitant birth defects (BDs) and chromosomal anomalies...
June 19, 2017: Birth defects research
https://www.readbyqxmd.com/read/28626737/high-level-bacterial-contamination-of-secondary-school-students-mobile-phones
#16
Siiri Kõljalg, Rando Mändar, Tiina Sõber, Tiiu Rööp, Reet Mändar
INTRODUCTION: While contamination of mobile phones in the hospital has been found to be common in several studies, little information about bacterial abundance on phones used in the community is available. Our aim was to quantitatively determine the bacterial contamination of secondary school students' mobile phones. METHODS: Altogether 27 mobile phones were studied. The contact plate method and microbial identification using MALDI-TOF mass spectrometer were used for culture studies...
June 2017: Germs
https://www.readbyqxmd.com/read/28624308/efficient-heterologous-expression-functional-characterization-and-molecular-modeling-of-annular-seabream-digestive-phospholipase-a2
#17
Nabil Smichi, Houcemeddine Othman, Neila Achouri, Alexandre Noiriel, Soumaya Triki, Vincent Arondel, Najet Srairi-Abid, Abdelkarim Abousalham, Youssef Gargouri, Nabil Miled, Ahmed Fendri
Here we report the cDNA cloning of a phospholipase A2 (PLA2) from five Sparidae species. The deduced amino acid sequences show high similarity with pancreatic PLA2. In addition, a phylogenetic tree derived from alignment of various available sequences revealed that Sparidae PLA2 are closer to avian PLA2 group IB than to mammals' ones. In order to understand the structure-function relationships of these enzymes, we report here the recombinant expression in E.coli, the refolding and characterization of His-tagged annular seabream PLA2 (AsPLA2)...
June 14, 2017: Chemistry and Physics of Lipids
https://www.readbyqxmd.com/read/28623701/transition-temperature-of-poly-methyl-methacrylate-determined-by-time-of-flight-secondary-ion-mass-spectrometry-and-contact-angle-measurements
#18
Yi Fu, Yiu-Ting R Lau, Lu-Tao Weng, Kai-Mo Ng, Chi-Ming Chan
The surface chain conformations of poly(methyl methacrylate) (PMMA) at different temperatures were extensively studied by time-of-flight secondary ion mass spectrometry (ToF-SIMS). Similar to our previous experimental studies on polystyrene (PS) and poly(2, 3, 4, 5, 6-pentafluorostyrene) (5FPS), a transition temperature (TT) could be identified through the principal component analysis (PCA) of the ToF-SIMS spectra obtained from the PMMA samples annealed at different temperatures. Interestingly, our results show that the TT depended on molecular weight and was about 50-60˚C below the bulk glass transition temperature (Tg) and therefore could possibly be related to the surface glass transition temperature (Tg(S))...
June 1, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28623565/the-novel-oleaginous-bacterium-sphingomonas-sp-egy1-dsm-29616-a-value-added-platform-for-renewable-biodiesel
#19
Nehad N Amer, Yasser Elbahloul, Amira M Embaby, Ahmed Hussein
Oleaginous microorganisms are regarded as efficient, renewable cell factories for lipid biosynthesis, a biodiesel precursor, to overwhelm the cosmopolitan energy crisis with affordable investment capital costs. Present research highlights production and characterization of lipids by a newly isolated oleaginous bacterium, Sphingomonas sp. EGY1 DSM 29616 through an eco-friendly approach. Only sweet whey [42.1% (v/v)] in tap water was efficiently used as a growth medium and lipid production medium to encourage cell growth and trigger lipid accumulation simultaneously...
July 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28623484/identification-of-mycobacterium-species-by-maldi-tof-mass-spectrometry
#20
M Neuschlova, M Vladarova, J Kompanikova, V Sadlonova, E Novakova
Matrix-assisted laser desorption ionization - time-of-flight (MALDI-TOF) mass spectrometry enables to identify microorganisms by comparison of the protein content with reference spectra in the database. The aim of this study was to evaluate the efficacy of phenotypic identification of mycobacteria by MALDI-TOF mass spectrometry in laboratory practice. Seventy five isolates of mycobacteria were identified by molecular and phenotypic method, and the results were compared by MALDI-TOF. For MALDI-TOF, material was processed according to the Bruker Daltonics protocol and Mycobacterial Library database version 2...
June 17, 2017: Advances in Experimental Medicine and Biology
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