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Tina Bernadette Angerer, Ylva Magnusson, Goran Landberg, John Stephen Fletcher
Breast cancer is an umbrella term used to describe a collection of different diseases with broad inter- and intra-tumor heterogeneity. Understanding this variation is critical in order to develop, and precisely prescribe, new treatments. Changes in the lipid metabolism of cancerous cells can provide important indications as to the metabolic state of the cells but are difficult to investigate with conventional histological methods. Due to the introduction of new higher energy (40 kV) gas cluster ion beams (GCIBs) time-of-flight secondary ion mass spectrometry (ToF-SIMS) imaging is now capable of providing information on the distribution of hundreds of molecular species simultaneously on a cellular to sub-cellular scale...
October 26, 2016: Analytical Chemistry
Ann-Sophie Schott, Jürgen Behr, Jennifer Quinn, Rudi F Vogel
Lactic acid bacteria (LAB) are widely used as starter cultures in the manufacture of foods. Upon preparation, these cultures undergo various stresses resulting in losses of survival and fitness. In order to find conditions for the subsequent identification of proteomic biomarkers and their exploitation for preconditioning of strains, we subjected Lactobacillus (Lb.) paracasei subsp. paracasei TMW 1.1434 (F19) to different stress qualities (osmotic stress, oxidative stress, temperature stress, pH stress and starvation stress)...
2016: PloS One
Taisuke Murata, Kenta Miwa, Noriaki Miyaji, Kei Wagatsuma, Tomoyuki Hasegawa, Keiichi Oda, Takuro Umeda, Takashi Iimori, Yoshitada Masuda, Takashi Terauchi, Mitsuru Koizumi
BACKGROUND: The point spread function (PSF) of positron emission tomography (PET) depends on the position across the field of view (FOV). Reconstruction based on PSF improves spatial resolution and quantitative accuracy. The present study aimed to quantify the effects of PSF correction as a function of the position of a traceable point-like (22)Na source over the FOV on two PET scanners with a different detector design. METHODS: We used Discovery 600 and Discovery 710 (GE Healthcare) PET scanners and traceable point-like (22)Na sources (<1 MBq) with a spherical absorber design that assures uniform angular distribution of the emitted annihilation photons...
December 2016: EJNMMI Physics
Eugénie Riesenkampff, Wietske Luining, Mike Seed, Paweena Chungsomprasong, Cedric Manlhiot, Bernadette Elders, Brian W McCrindle, Shi-Joon Yoo, Lars Grosse-Wortmann
BACKGROUND: Unfavorable left ventricular (LV) remodelling may be associated with adverse outcomes after Tetralogy of Fallot (TOF) repair. We sought to assess T1 cardiovascular magnetic resonance (CMR) markers of diffuse LV myocardial fibrosis in children after TOF repair, and associated factors. METHODS: In this prospective, cross-sectional study, native (=non-contrast) T1 times and extracellular volume fraction (ECV) were quantified in the LV myocardium using CMR...
October 26, 2016: Journal of Cardiovascular Magnetic Resonance
Tracy L Stockley, Amit M Oza, Hal K Berman, Natasha B Leighl, Jennifer J Knox, Frances A Shepherd, Eric X Chen, Monika K Krzyzanowska, Neesha Dhani, Anthony M Joshua, Ming-Sound Tsao, Stefano Serra, Blaise Clarke, Michael H Roehrl, Tong Zhang, Mahadeo A Sukhai, Nadia Califaretti, Mateya Trinkaus, Patricia Shaw, Theodorus van der Kwast, Lisa Wang, Carl Virtanen, Raymond H Kim, Albiruni R A Razak, Aaron R Hansen, Celeste Yu, Trevor J Pugh, Suzanne Kamel-Reid, Lillian L Siu, Philippe L Bedard
BACKGROUND: The clinical utility of molecular profiling of tumor tissue to guide treatment of patients with advanced solid tumors is unknown. Our objectives were to evaluate the frequency of genomic alterations, clinical "actionability" of somatic variants, enrollment in mutation-targeted or other clinical trials, and outcome of molecular profiling for advanced solid tumor patients at the Princess Margaret Cancer Centre (PM). METHODS: Patients with advanced solid tumors aged ≥18 years, good performance status, and archival tumor tissue available were prospectively consented...
October 25, 2016: Genome Medicine
Daniel Goldenberger, Vladimira Hinić, Spasenija Savic Prince, Michael Tamm, Anna-Maria Balestra, Doris Hohler, Reno Frei
BACKGROUND: Trichosporon mycotoxinivorans is a recently described yeast-like fungal organism and its association as a pathogen for patients with cystic fibrosis (CF) was reported previously. We show the clinical course of a CF patient over 9 years as well as the applications of modern molecular and proteomic identification techniques of this rare fungus. CASE PRESENTATION: We present the case of a 32-year-old male CF patient with sputum cultures continuously positive with the anamorphic yeast T...
October 25, 2016: BMC Infectious Diseases
Jindong Ren, Jianhong Hu, Li Chen, Yali Liu, Xiaoqin Xu, Jun He, Jianliang Shen, Lizhi Lu
Egg white proteins play an important role in avian reproductive systems and are an ideal resource for bioreactor construction. In this study, 1D electrophoresis and MALDI-TOF-MS were performed to analyze egg white proteins in four species. In total, 18, 11, 28, and 13 proteins were identified in the egg whites of the chicken, duck, goose, and pigeon, respectively. Egg white proteins in chickens have been studied previously; therefore, we focused on the proteins in goose and duck egg whites. Based on the amino acid sequence analysis and a comparison of the unique peptides, high similarity was observed between the goose and duck egg whites...
October 25, 2016: Protein and Peptide Letters
Sai Zheng, Jia-Chen Shi, Jian-Ying Hu, Wen-Xin Hu, Jing Zhang, Bing Shao
The reaction kinetics and transformation pathways between bisphenol F (BPF) and sodium hypochlorite were investigated at pH values ranging from 6.5 to 8.5 and with different initial concentration ratios. The reaction rate was pH- and free available chlorine (FAC)-dependent: the reaction rate at pH 8.5 was almost 10 times than that at pH 6.5. A total of 40 compounds were tentatively identified as chloro-substituted BPF and polyphenolic compounds by liquid chromatography quadrupole time-of-flight mass spectrometry operating in electrospray ionization mode (LC-ESI-Q-ToF), and 4 main byproducts were confirmed by (1)H and (13)C nuclear magnetic resonance (NMR)...
October 19, 2016: Water Research
Eun Ji Park, Dong Hee Na
The separation mechanism of PEGylated peptides in reversed-phase high-performance liquid chromatography (RP-HPLC) is complex, because the PEGylated molecules exhibit physicochemical properties that are different from those of the parent molecules and have heterogeneous structure. Since most separation studies have focused on the hydrophobicity of the peptide relative to poly(ethylene glycol) (PEG), the role of PEG in the separation of PEGylated peptides on RP-HPLC is not clear. To elucidate the effect of the attached PEG on the retention of PEGylated peptides on RP-HPLC, the mono-PEGylated forms of collagen pentapeptide and octreotide were fractionated drop-by-drop from the outlet of the HPLC system and each drop was subjected to matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)...
October 25, 2016: Analytical Chemistry
Xingkai An, Jie Fang, Qing Lin, Congxia Lu, Qilin Ma, Hongli Qu
Migraine is a common and disabling nervous system disease with a significant genetic predisposition. The sex hormones play an important role in the pathogenesis of migraine. However, the conclusions of the previous genetic relation studies are conflicting. The aim of this study is to determine whether variants in genes involved in estrogen receptor and estrogen hormone metabolism are related to Chinese migraine. By employing a case-control approach, 8 SNPs in the ESR1, ESR2, and CYP19A1 genes are studied in a cohort of 494 migraine cases and 533 controls...
October 24, 2016: Journal of Neurology
Silke Schelenz, Ferry Hagen, Johanna L Rhodes, Alireza Abdolrasouli, Anuradha Chowdhary, Anne Hall, Lisa Ryan, Joanne Shackleton, Richard Trimlett, Jacques F Meis, Darius Armstrong-James, Matthew C Fisher
BACKGROUND: Candida auris is a globally emerging multidrug resistant fungal pathogen causing nosocomial transmission. We report an ongoing outbreak of C. auris in a London cardio-thoracic center between April 2015 and July 2016. This is the first report of C. auris in Europe and the largest outbreak so far. We describe the identification, investigation and implementation of control measures. METHODS: Data on C. auris case demographics, environmental screening, implementation of infection prevention/control measures, and antifungal susceptibility of patient isolates were prospectively recorded then analysed retrospectively...
2016: Antimicrobial Resistance and Infection Control
Dijun Zhang, Weina He, Qianqian Tong, Jun Zhou, Xiurong Su
BACKGROUND: The acquisition of iron is important for the pathogenicity of bacteria and blood. Three different culture environments (Fe stimulation, blood agar plate and normal plate) were used to stimulate Enterobacter cloacae, and their respective pathogenicities were compared at the proteomic, mRNA and metabolomic levels. METHODS: 2D-DIGE combined with MALDI-TOF-MS/MS, RT-PCR and (1)H NMR were used to analyze the differential expression levels of proteins, mRNA and metabolites...
2016: Proteome Science
Tomasz Mroczek, Tomasz Plech, Monika Wujec
Till now, three major spectroscopic techniques, fourier transform infrared (FT-IR), nuclear magnetic resonance (NMR) spectroscopy and X-ray crystallography, have been used for determination of thiol-thione tautomeric forms. Therefore, a novel analytical concept of discrimination of 1,2,4-triazole-3-thione and 3-thiol tautomers using two high-resolution mass spectrometers has been proposed. It comprises the high-performance liquid chromatography (HPLC) hyphenated with electrospray ionization (ESI)-time-of-flight mass spectrometry (ESI-TOF-MS, positive ion mode) followed by in-source collision-induced dissociation (CID) and low-energy CID-tandem mass spectrometry (MS/MS) analysis measured with a Quadruple-TOF-MS instrument...
October 24, 2016: Journal of Chromatographic Science
Graziano Colombo, Marco Clerici, Alessandra Altomare, Francesco Rusconi, Daniela Giustarini, Nicola Portinaro, Maria Lisa Garavaglia, Ranieri Rossi, Isabella Dalle-Donne, Aldo Milzani
: In this study, we assessed the oxidative damage occurring in plasma proteins when human blood was exposed to inflammatory concentrations of hypochlorous acid (HOCl). We used specific thiol labelling and Western blot analyses to determine protein thiol oxidation, as well as analytical gel filtration HPLC coupled to fluorescence detection to explore formation of high molecular weight (HMW) protein aggregates. Thiol-containing proteins oxidized by HOCl were identified by redox proteomics...
October 21, 2016: Journal of Proteomics
Svetlana A Ivanova, Diana Z Osmanova, Anastasia S Boiko, Ivan V Pozhidaev, Maxim B Freidin, Olga Yu Fedorenko, Arkadiy V Semke, Nikolay A Bokhan, Elena G Kornetova, Lubov D Rakhmazova, Bob Wilffert, Anton J M Loonen
BACKGROUND: Antipsychotic drugs can cause hyperprolactinemia. However, hyperprolactinemia was also observed in treatment-naive patients with a first schizophrenic episode. This phenomenon might be related to the role of prolactin as a cytokine in autoimmune diseases. Extrapituitary prolactin production is regulated by an alternative promoter, which contains the functional single nucleotide polymorphism -1149 G/T (rs1341239). We examined whether this polymorphism was associated with hyperprolactinemia in patients with schizophrenia...
October 21, 2016: Schizophrenia Research
Jie Yang, Yonghui Lin, Xiaodan Yang, Tzi Bun Ng, Xiuyun Ye, Juan Lin
Magnetic cross-linked enzyme aggregates (M-CLEAs) were prepared for Cerrena laccase and used in antibiotic treatment. Of the seven antibiotics examined in this study, Cerrena laccase M-CLEAs were most effective in degradation of tetracycline (TC) and oxytetracycline (OTC), followed by ampicillin, sulfamethoxazole and erythromycin. The redox mediator ABTS was not able to improve efficiencies of degradation of TC and OTC. Cerrena laccase at 40U/mL eliminated 100μg/mL TC at pH 6 and 25°C in 48h in the absence of a redox mediator, with over 80% degradation occurring within the first 12h...
October 11, 2016: Journal of Hazardous Materials
Lihui Men, Zifeng Pi, Yuan Zhou, Mengying Wei, Yuanyuan Liu, Fengrui Song, Zhongying Liu
Obesity has become a global epidemic and public health challenge which associates with serious health issues including diabetes, cardiovascular disease, stroke, arthritis, and some types of cancer. To better understand obesity and obesity-related dysfunction, a high-fat diet (HFD) induced obese model was developed on Sprague-Dawley rats. Metabolomics based on ultra high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) was untilized to identify and analyze obesity related metabolites in rat urine samples...
October 14, 2016: Journal of Pharmaceutical and Biomedical Analysis
Ben Ajmia Wafa, Mohamed Makni, Sonda Ammar, Lamia Khannous, Amal Ben Hassana, Mohamed Bouaziz, Nour Eddine Es-Safi, Radhouane Gdoura
Punica granatum L. is widely recognized for its potency against a broad spectrum of bacterial pathogens. The purpose of this study was to explore the inhibitory and the bactericidal activities of Punica granatum against Salmonella strains. The effect of extracts obtained from different parts (peels, seeds, juice and flowers) of pomegranate and using different solvents against Salmonella enterica serovars Kentucky and Enteritidis isolated from chicken meat was thus investigated. Salmonella strains were identified with the standard API-20E system and confirmed by real time PCR...
October 11, 2016: International Journal of Food Microbiology
Junhui Chen, Xin Li, Shuai Wang, Farong Chen, Wei Cao, Chengjun Sun, Li Zheng, Xiaoru Wang
This study aimed to develop an exact mass suspect screening approach to perform finding of multiple lipophilic marine toxins (LMTs) in seawater, suspended particulate matter (SPM), and marine sediment from marine aquaculture area using liquid chromatography-time of flight mass spectrometry (LC-TOF/MS). The method was validated and proven to be reliable for the screening of various LMTs. Then, the method was applied to screen LMTs in marine environmental samples collected from mariculture area of Jiaozhou Bay, China...
October 21, 2016: Chemosphere
Pedro J Alcolea, Ana Alonso, Francisco García-Tabares, María C Mena, Sergio Ciordia, Vicente Larraga
Leishmania amazonensis is one of the major etiological agents of the neglected, stigmatizing disease termed american cutaneous leishmaniasis (ACL). ACL is a zoonosis and rodents are the main reservoirs. Most cases of ACL are reported in Brazil, Bolivia, Colombia and Peru. The biological cycle of the parasite is digenetic because sand fly vectors transmit the motile promastigote stage to the mammalian host dermis during blood meal intakes. The amastigote stage survives within phagocytes of the mammalian host...
2016: PloS One
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