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https://www.readbyqxmd.com/read/28527414/structural-characterization-and-discrimination-of-the-paris-polyphylla-var-yunnanensis-and-paris-vietnamensis-based-on-metabolite-profiling-analysis
#1
Li-Ping Kang, Yuan-Yuan Huang, Zhi-Lai Zhan, Da-Hui Liu, Hua-Sheng Peng, Tie-Gui Nan, Yuan Zhang, Qing-Xiu Hao, Jin-Fu Tang, Shou-Dong Zhu, Guang Yang, Lan-Ping Guo, Min Chen, Lu-Qi Huang
This study aimed to distinguish the rhizomes of Paris polyphylla var. yunnanensis (Franch) Hand Mazz (PPY) and Paris veitnamensis (Takht.) H. Li (PV) using metabolomics-based ultra high-performance liquid chromatography coupled with quadrupole time-of-fligh mass spectrometry (UHPLC/Q-TOF MS). First, the UHPLC/Q-TOF MS approach was optimized for metabolite profiling. Then, the MS data were processed using UNIFI™ combined with an in-house library to automatically characterize the metabolites. Based on the exact mass information, the fragmentation characteristics, and the retention time of compounds, and the fragmentation mechanism and retention behavior of steroidal glycosides in the references, the structures identified by UNIFI were further verified...
May 11, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28527370/atherosclerotic-dyslipidemia-revealed-by-plasma-lipidomics-on-apoe-mice-fed-a-high-fat-diet
#2
Yanyan Chen, Shiyuan Wen, Miaomiao Jiang, Yan Zhu, Liqin Ding, Hong Shi, Pengzhi Dong, Jing Yang, Yue Yang
BACKGROUND AND AIMS: The pathological process of atherosclerosis is closely related to lipid accumulation at arterial wall caused by lipoproteins transporting lipids through blood. In this study, we investigate the lipid composition in the plasma of apolipoprotein E deficient (ApoE(-/-)) mice fed a high-fat diet to reveal atherosclerosis-induced dyslipidemia. METHODS: ApoE(-/-) and corresponding wild-type C57BL/6J mice were used as the pathology model and control, respectively, and were fed a high-fat or normal diet...
May 11, 2017: Atherosclerosis
https://www.readbyqxmd.com/read/28526845/chemical-structure-of-the-lipid-a-component-of-pseudomonas-sp-strain-pamc-28618-from-thawing-permafrost-in-relation-to-pathogenicity
#3
Han-Gyu Park, Ganesan Sathiyanarayanan, Cheol-Hwan Hwang, Da-Hee Ann, Jung-Ho Kim, Geul Bang, Kyoung-Soon Jang, Hee Wook Ryu, Yoo Kyung Lee, Yung-Hun Yang, Yun-Gon Kim
Climate change causes permafrost thawing, and we are confronted with the unpredictable risk of newly discovered permafrost microbes that have disease-causing capabilities. Here, we first characterized the detailed chemical structure of the lipid A moiety from a Pseudomonas species that was isolated from thawing arctic permafrost using MALDI-based mass spectrometric approaches (i.e., MALDI-TOF MS and MALDI-QIT-TOF MS(n)). The MALDI multi-stage mass spectrometry (MS) analysis of lipid A extracted from the Pseudomonas sp...
May 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28525645/molecular-characterization-of-prototheca-strains-isolated-in-china-revealed-the-first-cases-of-protothecosis-associated-with-prototheca-zopfii-genotype-1
#4
Noriyuki Hirose, Zhensheng Hua, Yuichi Kato, Qiangqiang Zhang, Ruoyu Li, Kazuko Nishimura, Michiaki Masuda
In this study, six strains of Prototheca isolated in China from human patients diagnosed as protothecosis and cows with mastitis were characterized by polymerase chain reaction (PCR) and nucleotide sequencing of the ribosomal RNA gene (rDNA) and by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS). The results indicated that three strains isolated from the human patients were P. zopfii genotype 1, revealing the first cases of human protothecosis associated with P. zopfii genotype 1...
May 19, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/28523732/long-term-respiratory-outcomes-after-primary-total-correction-for-tetralogy-of-fallot-and-absent-pulmonary-valve-in-patient-with-respiratory-symptoms
#5
Takaya Hoashi, Toru Iwasa, Koji Kagisaki, Masatoshi Shimada, Kenichi Kurosaki, Isao Shiraishi, Hajime Ichikawa
OBJECTIVE: To review long-term respiratory outcomes for tetralogy of Fallot and absent pulmonary valve (TOF/APV) in respiratory symptomatic populations. METHODS: Of 25 consecutive patients undergoing primary total correction for TOF/APV between 1987 and 2016, Sixteen patients (64%) with a preoperative respiratory disturbance were enrolled. The median age at operation was 1.9 months old, including 4 neonates and 12 infants. Ten patients (62.5%) preoperatively necessitated mechanical ventilator support...
May 19, 2017: Congenital Heart Disease
https://www.readbyqxmd.com/read/28523582/first-in-human-radiation-dosimetry-of-68-ga-nodaga-rgdyk
#6
Silvano Gnesin, Periklis Mitsakis, Francesco Cicone, Emmanuel Deshayes, Vincent Dunet, Augusto F Gallino, Marek Kosinski, Sébastien Baechler, Franz Buchegger, David Viertl, John O Prior
BACKGROUND: Integrin-targeting radiopharmaceuticals have potential broad applications, spanning from cancer theranostics to cardiovascular diseases. We have previously reported preclinical dosimetry results of (68)Ga-NODAGA-RGDyK in mice. This study presents the first human dosimetry of (68)Ga-NODAGA-RGDyK in the five consecutive patients included in a clinical imaging protocol of carotid atherosclerotic plaques. Five male patients underwent whole-body time-of-flight (TOF) PET/CT scans 10, 60 and 120 min after tracer injection (200 MBq)...
December 2017: EJNMMI Research
https://www.readbyqxmd.com/read/28523576/proteomic-analysis-of-serum-amyloid-a-as-a-potential-marker-in-intestinal-beh%C3%A3-et-s-disease
#7
Hyun Jung Lee, Jae Hyun Kim, Seung Won Kim, Hyun Ah Joo, Hye Won Lee, You Sun Kim, Soo Jung Park, Sung Pil Hong, Tae Il Kim, Won Ho Kim, Young-Ho Kim, Jae Hee Cheon
BACKGROUND/AIMS: Data regarding biomarkers to understand disease pathogenesis and to assess disease activity of intestinal Behçet's disease (BD) are limited. Therefore, we aimed to investigate the differentially expressed proteins in sera from patients with intestinal BD and to search for biomarkers using mass spectrometry-based proteomic analysis. METHODS: Serum samples were pooled for the screening study, and two-dimensional electrophoresis (2-DE) was performed to characterize the proteins present in intestinal BD patients...
May 18, 2017: Digestive Diseases and Sciences
https://www.readbyqxmd.com/read/28523507/tof-sims-parallel-imaging-ms-ms-of-lipid-species-in-thin-tissue-sections
#8
Anne Lisa Bruinen, Gregory L Fisher, Ron M A Heeren
Unambiguous identification of detected species is essential in complex biomedical samples. To date, there are not many mass spectrometry imaging techniques that can provide both high spatial resolution and identification capabilities. A new and patented imaging tandem mass spectrometer, exploiting the unique characteristics of the nanoTOF II (Physical Electronics, USA) TOF-SIMS TRIFT instrument, was developed to address this.Tandem mass spectrometry is based on the selection of precursor ions from the full secondary ion spectrum (MS(1)), followed by energetic activation and fragmentation, and collection of the fragment ions to obtain a tandem MS spectrum (MS(2))...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28523501/peptide-imaging-maximizing-peptide-yield-optimization-of-the-peptide-mass-fingerprint
#9
Ekta Patel
In Matrix Assisted Laser Desorption Ionization Mass Spectrometry Imaging (MALDI-MSI), identification of observed proteins remains a challenge primarily due to the drop in sensitivity of time-of-flight (TOF) mass spectrometers beyond the mass range of 25-35 kDa (Francese et al. High Throughput Screen 12: 156-174, 2009) and inadequate mass resolving power at those molecular weights. To counteract this, a bottom-up proteomic approach is often employed. Proteolysis digests proteins into smaller peptide fragments, typically between 500 and 3000 Da which are easier to detect with a higher mass accuracy...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28523500/desi-mass-spectrometry-imaging-msi
#10
Emmanuelle Claude, Emrys A Jones, Steven D Pringle
Desorption Electrospray Ionization (DESI) mass spectrometry is a technique that allows chemical information to be obtained directly from a wide range of surfaces. Using a 2D stage, DESI can be implemented in an imaging mode whereby MS spectra are collected by rastering the spray across the whole surface. Here, we describe the implementation and optimization of DESI imaging for metabolites and lipids from tissue sections using oa-TOF mass spectrometers.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28523113/human-serum-albumin-delivered-au-pet3-is-a-potent-inhibitor-of-t-cell-proliferation
#11
Tyler C Dean, Mu Yang, Mingyong Liu, Jason M Grayson, Anthony W DeMartino, Cynthia S Day, Jingyun Lee, Cristina M Furdui, Ulrich Bierbach
Using a modular library format in conjunction with cell viability (MTS) and flow cytometry assays, 90 cationic complexes [AuPL] (n+) (P = phosphine ligand; L = thiourea derivative or chloride) were studied for their antiproliferative activity in CD8(+) T lymphocyte cells. The activity of the compounds correlates with the steric bulk of the phosphine ligands. Thiourea serves as a leaving group that is readily replaced by cysteine thiol (NMR, ESI-MS). Taking advantage of selective thiourea ligand exchange, the fragments [Au(PEt3)](+) and [Au(JohnPhos)](+) (JohnPhos = 1,1'-biphenyl-2-yl)di-tert-butylphosphine) in compounds 1 and 2 were transferred to recombinant human serum albumin (rHSA)...
May 11, 2017: ACS Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28522973/profiling-and-distribution-of-metabolites-of-procyanidin-b2-in-mice-by-uplc-dad-esi-it-tof-ms-n-technique
#12
Ying Xiao, Zhongzhi Hu, Zhiting Yin, Yiming Zhou, Taiyi Liu, Xiaoli Zhou, Dawei Chang
The metabolite profiles and distributions of procyanidin B2 were qualitatively described using UPLC-DAD-ESI-IT-TOF-MS(n) without help of reference standards, and a possible metabolic pathway was proposed in the present study. Summarily, 53 metabolites (24 new metabolites) were detected as metabolites of procyanidin B2, and 45 of them were tentatively identified. Twenty seven metabolites were assigned as similar metabolites of (-)-epicatechin by scission of the flavanol interflavanic bond C4-C8, including 16 aromatic metabolites, 5 conjugated metabolites, 3 ring-cleavage metabolites, and 2 phenylvalerolactone metabolites...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28522664/non-contrast-enhanced-silent-scan-mr-angiography-of-intracranial-anterior-circulation-aneurysms-treated-with-a-low-profile-visualized-intraluminal-support-device
#13
N Takano, M Suzuki, R Irie, M Yamamoto, K Teranishi, K Yatomi, N Hamasaki, K K Kumamaru, M Hori, H Oishi, S Aoki
BACKGROUND AND PURPOSE: The Low-Profile Visualized Intraluminal Support Device comprises a small-cell nitinol structure and a single-wire braided stent that provides greater metal coverage than previously reported intracranial stents, as well as assumed strong susceptibility artifacts. This study aimed to assess the benefits of non-contrast-enhanced MRA by using a Silent Scan (Silent MRA) for intracranial anterior circulation aneurysms treated with Low-Profile Visualized Intraluminal Support Device stents...
May 18, 2017: AJNR. American Journal of Neuroradiology
https://www.readbyqxmd.com/read/28522339/proteomics-analysis-of-fusarium-proliferatum-under-various-initial-ph-during-fumonisin-production
#14
Taotao Li, Liang Gong, Yong Wang, Feng Chen, Vijai Kumar Gupta, Qijie Jian, Xuewu Duan, Yueming Jiang
Fusarium proliferatum as a fungal pathogen can produce fumonisin which causes a great threat to animal and human health. Proteomic approach was a useful tool for investigation into mycotoxin biosynthesis in fungal pathogens. In this study, we analyzed the fumonisin content and mycelium proteins of Fusarium proliferatum cultivated under the initial pH5 and 10. Fumonisin production after 10days was significantly induced in culture condition at pH10 than pH5. Ninety nine significantly differently accumulated protein spots under the two pH conditions were detected using two dimensional polyacrylamide gel electrophoresis and 89 of these proteins were successfully identified by MALDI-TOF/TOF and LC-ESI-MS/MS analysis...
May 15, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28521505/orientation-and-conformation-of-osteocalcin-adsorbed-onto-calcium-phosphate-and-silica-surfaces
#15
Luisa A Scudeller, Selvi Srinivasan, Alexandre M Rossi, Patrick S Stayton, Gary P Drobny, David G Castner
Adsorption isotherms, circular dichroism (CD) spectroscopy, x-ray photoelectron spectroscopy (XPS), and time-of-flight secondary ion mass spectrometry (ToF-SIMS) were used to investigate the adsorption of human osteocalcin (hOC) and decarboxylated (i.e., Gla converted back to Glu) hOC (dhOC) onto various calcium phosphate surfaces as well as silica surfaces. The adsorption isotherms and XPS nitrogen signals were used to track the amount of adsorbed hOC and dhOC. The intensities of key ToF-SIMS amino acid fragments were used to assess changes in the structure of adsorbed hOC and dhOC...
May 18, 2017: Biointerphases
https://www.readbyqxmd.com/read/28521273/rapid-discovery-of-cyclopamine-analogs-from-fritillaria-and-veratrum-plants-using-lc-q-tof-ms-and-lc-qqq-ms
#16
Yuan Du, Zu-Guo Zheng, Yue Yu, Zi-Tian Wu, Dan Liang, Ping Li, Yan Jiang, Hui-Jun Li
Cyclopamine, an inhibitor of the Hedgehog (Hh) signaling pathway, has been paid much attention in treating a wide variety of tumors. However, isolation and purification of cyclopamine analogs from medicinal plants remain challengeable. We herein proposed an efficient strategy using liquid chromatography quadrupole-time-of-flight mass spectrometry (LC-Q-TOF-MS) and liquid chromatography triple-quadrupole mass spectrometry (LC-QqQ-MS) for rapid screening and targeted isolation of cyclopamine analogs in Fritillaria and Veratrum plants...
May 1, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28520776/proteomic-analysis-of-tea-plants-camellia-sinensis-with-purple-young-shoots-during-leaf-development
#17
Qiongqiong Zhou, Zhidan Chen, Jinwook Lee, Xinghui Li, Weijiang Sun
Tea products made from purple leaves are highly preferred by consumers due to the health benefits. This study developed a proteome reference map related to color changes during leaf growth in tea (Camellia sinensis) plant with purple young shoots using two-dimensional electrophoresis (2-DE). Forty-six differentially expressed proteins were detected in the gel and successfully identified by using MALDI-TOF/TOF-MS. The pronounced changes in the proteomic profile between tender purple leaves (TPL) and mature green leaves (MGL) included: 1) the lower activity of proteins associated with CO2 assimilation, energy metabolism and photo flux efficiency and higher content of anthocyanins in TPL than those in MGL may protect tender leaves against photo-damage; 2) the higher abundance of chalcone synthase (CHS), chalcone isomerase (CHI) and flavonol synthase (FLS) likely contributes to the synthesis of anthocyanins, catechins and flavonols in TPL tissues; 3) higher abundance of stress response proteins, such as glutathione S-transferases (GST) and phospholipid hydroperoxide glutathione peroxidase (PHGPx), could enhance the tolerance of TPL tissues to adverse condition in; and 4) the increased abundance of proteins related to protein synthesis, nucleic acids and cell wall proteins should be beneficial for the proliferation and expansion of leaf cell in TPL tissues...
2017: PloS One
https://www.readbyqxmd.com/read/28520558/data-driven-event-by-event-respiratory-motion-correction-using-tof-pet-list-mode-centroid-of-distribution
#18
Silin Ren, Xiao Jin, Chung Chan, Yiqiang Jian, Tim Mulnix, Chi Liu, Richard E Carson
Data-driven respiratory gating techniques were developed to correct for respiratory motion in PET studies, without the help of external motion tracking systems. Due to the greatly increased image noise in gated reconstructions, it is desirable to develop a data-driven event-by-event respiratory motion correction method. In this study, using the Centroid-of-distribution (COD) algorithm, we established a data-driven event-by-event respiratory motion correction technique using TOF PET list-mode data, and investigated its performance by comparing with an external system-based correction method...
May 18, 2017: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/28520473/a-new-method-and-mass-spectrometric-instrument-for-extraterrestrial-microbial-life-detection-using-the-elemental-composition-analyses-of-martian-regolith-and-permafrost-ice
#19
G G Managadze, A A Safronova, K A Luchnikov, E A Vorobyova, N S Duxbury, P Wurz, N G Managadze, A E Chumikov, R Kh Khamizov
We propose a new technique for the detection of microorganisms by elemental composition analyses of a sample extracted from regolith, permafrost, and ice of extraterrestrial bodies. We also describe the design of the ABIMAS instrument, which consists of the onboard time-of-flight laser mass-reflectron (TOF LMR) and the sample preparation unit (SPU) for biomass extraction. This instrument was initially approved to fly on board the ExoMars 2020 lander mission. The instrument can be used to analyze the elemental composition of possible extraterrestrial microbial communities and compare it to that of terrestrial microorganisms...
May 2017: Astrobiology
https://www.readbyqxmd.com/read/28520413/temporal-characteristics-of-brown-carbon-over-the-central-indo-gangetic-plain
#20
Rangu Venkata Satish, Puthukkadan Moosakutty Shamjad, Navaneeth M Thamban, Sachchida N Tripathi, Neeraj Rastogi
Recent global models estimate that light absorption by brown carbon (BrC) in several regions of the world is ~30-70% of that due to black carbon (BC). It is, therefore, important to understand its sources and characteristics on temporal and spatial scale. In this study, we conducted semi-continuous measurements of water-soluble organic carbon (WSOC) and BrC using particle-into-liquid sampler coupled with liquid waveguide capillary cell and total carbon analyzer (PILS-LWCC-TOC) over Kanpur (26.5°N, 80.3°E, 142 m amsl) during a winter season (December 2015 to February 2016)...
May 18, 2017: Environmental Science & Technology
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