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https://www.readbyqxmd.com/read/29684765/application-of-maldi-tof-ms-for-the-subtyping-of-arcobacter-butzleri-strains-and-comparison-with-their-mlst-and-pfge-types
#1
Federica Giacometti, Silvia Piva, Katleen Vranckx, Katrien De Bruyne, Ilenia Drigo, Alex Lucchi, Gerardo Manfreda, Andrea Serraino
For the first time, this study evaluated the use of MALDI-TOF as a typing tool for Arcobacter butzleri. A total of 104 A. butzleri strains isolated from different sources in an artisanal dairy plant in Italy were identified and typed using MALDI-TOF and compared with their multilocus sequence typing (MLST) and pulsed field gel electrophoresis (PFGE) profiles found in previous studies. MALDI-TOF correctly identified all the isolates to species level. No clearly delineated clusters appeared on dendrograms based on either the complete spectra or the significant peaks, but nine clusters were defined using the cophenetic correlation...
April 16, 2018: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29683664/assessment-of-the-acute-toxicity-uptake-and-biotransformation-potential-of-benzotriazoles-in-zebrafish-danio-rerio-larvae-combining-hilic-with-rplc-hrms-for-high-throughput-identification
#2
Dimitrios E Damalas, Anna A Bletsou, Adamantia Agalou, Dimitris Beis, Nikolaos S Thomaidis
The current study reports on the toxicity, uptake and the biotransformation potential of zebrafish (embryos and larvae) exposed to Benzotriazoles (BTs). Acute toxicity assays were conducted. Cardiac function abnormalities (pericardial edema and poor blood circulation) were observed from the phenotypic analysis of early life zebrafish embryos after BTs exposure. For the uptake and biotransformation experiment, extracts of whole body larvae were analyzed using liquid chromatography-high-resolution tandem mass spectrometry (UPLC-Q-TOF-HRMS/MS)...
April 23, 2018: Environmental Science & Technology
https://www.readbyqxmd.com/read/29683064/genetic-variants-in-mirna-machinery-genes-associated-with-clinicopathological-characteristics-and-outcomes-of-gastric-cancer-patients
#3
Yuqian Liao, Yulu Liao, Jun Li, Liyan Liu, Junyu Li, Yiye Wan, Lixiang Peng
BACKGROUND: Polymorphisms in miRNA machinery genes have been proved to be related to risk or survival of several kinds of cancers, but the results are controversial and the role of these polymorphisms in gastric cancer remains uncertain. In our study, we investigated the association between five genetic variants in miRNA machinery genes ( DICER, RAN, XPO5 [name of the gene]) and clinical outcomes in Chinese gastric cancer patients. METHODS: A total of 96 patients with stage IB-III gastric cancer treated with radical gastrectomy and adjuvant chemotherapy of oxaliplatin and fluorouracils were analyzed...
April 1, 2018: International Journal of Biological Markers
https://www.readbyqxmd.com/read/29683008/first-trimester-combined-screening-biochemistry-in-detection-of-congenital-heart-defects
#4
Julia Alanen, Teemu Korpimaki, Heikki Kouru, Mikko Sairanen, Markku Leskinen, Mika Gissler, Markku Ryynanen, Jaana Nevalainen
OBJECTIVE: To evaluate the performance of first trimester biochemical markers, pregnancy-associated plasma protein-A (PAPP-A), free beta human chorionic gonadotropin (fβ-hCG), and nuchal translucency (NT) in detection of severe congenital heart defects (CHDs). METHODS: During the study period from 1 January 2008 to 31 December 2011, biochemical markers and NT were measured in 31,144 women as part of voluntary first trimester screening program for Down's syndrome in Northern Finland...
April 22, 2018: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/29681980/anti-helicobacter-pylori-and-anti-inflammatory-effects-and-constituent-analysis-of-modified-xiaochaihutang-for-the-treatment-of-chronic-gastritis-and-gastric-ulcer
#5
Xin Chen, Lijuan Hu, Huanhuan Wu, Wei Liu, Shuhe Chen, Aijun Zhou, Yanwen Liu
Chronic gastritis and gastric ulcers are prevalent throughout the world and are considered to be a global health problem. Modified Xiaochaihutang (MXCHT) prescription is broadly used in traditional medicine hospital for the treatment of gastritis. In order to assess the anti- Helicobacter pylori (H. pylori) effect of MXCHT, agar diffusion method in vitro and fluid dilution method for the minimal inhibitory concentration (MIC) were established. The anti-inflammatory effects were then evaluated using mouse ear edema model and rat paw edema model...
2018: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/29681970/-k-nearest-neighbor-algorithm-coupled-with-metabonomics-to-study-the-therapeutic-mechanism-of-sendeng-4-in-adjuvant-induced-rheumatoid-arthritis-rat
#6
Xiye Wang, Dan Li, Mingyang Jiang, Zhili Pei, Liang Xu
As a traditional Mongolian medicine, Sendeng-4 (SD) has been widely used to treat rheumatoid arthritis (RA) in Inner Mongolia and exhibits a good curative effect. Unfortunately, due to geographical factors, it is difficult to popularize this drug throughout the whole country, and the mechanism of action of SD has been unclear. In this study, a serum metabolite profile analysis was performed to identify potential biomarkers associated with adjuvant-induced RA and investigate the mechanism of action of SD. Ultraperformance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) was performed for the metabonomics analysis...
2018: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/29681784/comparison-among-reconstruction-algorithms-for-quantitative-analysis-of-11-c-acetate-cardiac-pet-imaging
#7
Ximin Shi, Nan Li, Haiyan Ding, Yonghong Dang, Guilan Hu, Shuai Liu, Jie Cui, Yue Zhang, Fang Li, Hui Zhang, Li Huo
Objective: Kinetic modeling of dynamic 11 C-acetate PET imaging provides quantitative information for myocardium assessment. The quality and quantitation of PET images are known to be dependent on PET reconstruction methods. This study aims to investigate the impacts of reconstruction algorithms on the quantitative analysis of dynamic 11 C-acetate cardiac PET imaging. Methods: Suspected alcoholic cardiomyopathy patients ( N = 24) underwent 11 C-acetate dynamic PET imaging after low dose CT scan...
2018: Contrast Media & Molecular Imaging
https://www.readbyqxmd.com/read/29680302/chemical-profiling-and-the-potential-active-constituents-responsible-for-wound-healing-in-periploca-forrestii-schltr
#8
Lei Chen, Jinsong Li, Xuan Ke, Cailing Sun, Xiaoxian Huang, Pan Jiang, Feng Feng, Jie Zhang, Wenyuan Liu
ETHNOPHARMACOLOGICAL RELEVANCE: Periploca forrestii Schltr. is a popular folk medicine in china, commonly prescribed for the treatment of rheumatoid arthritis and wounds. The present research aimed to evaluate the effects of HLG on wound healing and reveal the potential active constituents. MATERIALS AND METHODS: The wound healing activity was assessed by proliferation of fibroblast, migration and collagen production using L929 cells. A reliable HPLC-Q-TOF-MS/MS method was constructed for the systematic identification and characterization of main components in HLG...
April 19, 2018: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29680057/the-effects-of-boiling-and-fermentation-on-betalain-profiles-and-antioxidant-capacities-of-red-beetroot-products
#9
Tomasz Sawicki, Wiesław Wiczkowski
The aim of study was investigation the impact of boiling and spontaneous fermentation on profile and content of betalains and antioxidant capacity of red beetroot. Betalains were analyzed by micro-HPLC-TOF-MS/MS method, while antioxidant capacity by three in vitro assays. Red beet products were abounded in betalains, with betanin, isobetanin, betanidin and vulgaxanthin I predominating among twenty two pigments identified. Boiling and fermentation of red beet reduced the content of betalains by 51-61% and 61-88%, respectively, however, this decline was limited when the peel was present...
September 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29679549/chm-1-a-novel-microtubule-destabilizing-agent-exhibits-antitumor-activity-via-inducing-the-expression-of-sirt2-in-human-breast-cancer-cells
#10
Chin-Wei Liu, Ying-Chao Lin, Chao-Ming Hung, Bing-Lan Liu, Sheng-Chu Kuo, Chi-Tang Ho, Tzong-Der Way, Chih-Hsin Hung
Breast cancer is a major public health problem throughout the world. In this report, we investigated whether CHM-1, a novel synthetic antimitotic agent could be developed into a potent antitumor agent for treating human breast cancer. CHM-1 induced growth inhibition in MDA-MB-231, MDA-MB-453 and MCF-7 cells in a concentration-dependent manner. Importantly, CHM-1 is less toxic to normal breast (HBL-100) cells. CHM-1 interacted with tubulin, markedly inhibited tubulin polymerization, and disrupted microtubule organization...
April 18, 2018: Chemico-biological Interactions
https://www.readbyqxmd.com/read/29679385/genus-level-identification-of-dermatophytes-by-maldi-tof-ms-after-two-days-of-colony-growth
#11
Jari Intra, Cecilia Sarto, Natalia Tiberti, Silvia Besana, Cinzia Savarino, Paolo Brambilla
Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) is becoming a popular technology in clinical microbiology. It is a fast and highly specific method for the routine identification of microorganisms. In this study, we evaluated the suitability of dermatophyte identification after only two days of colony growth using MALDI-TOF MS. Two protein extraction protocols were also evaluated consisting of either formic acid alone or of ethanol-formic acid-acetonitrile to achieve a complete protein extraction...
April 21, 2018: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29678480/gram-negative-anaerobe-susceptibility-testing-in-clinical-isolates-using-sensititre-and-etest-methods
#12
C Hughes, C Ashhurst-Smith, J K Ferguson
Gram negative anaerobic organisms are important pathogens in a range of clinical infections, and susceptibility testing is not commonly performed in the microbiology laboratory. We performed anaerobic susceptibility testing on 70 clinically relevant Gram negative anaerobes isolated from routine cultures in a busy diagnostic laboratory which were identified by MALDI-TOF mass spectrometry (MALDI-TOF MS). The susceptibility testing was performed by two methods: Sensititre trays (ThermoFisher Scientific) against 15 different antibiotics, and Etests (bioMérieux) against five clinically relevant antibiotics (metronidazole, piperacillin-tazobactam, amoxicillin-clavulanate, meropenem and clindamycin)...
April 17, 2018: Pathology
https://www.readbyqxmd.com/read/29678339/usefulness-of-pulmonary-arterial-end-diastolic-forward-flow-late-after-tetralogy-of-fallot-repair-to-predict-a-restrictive-right-ventricle
#13
Shelby Kutty, Anne Marie Valente, Matthew T White, Kelsey Hickey, David A Danford, Andrew J Powell, Tal Geva
The functional significance of pulmonary arterial end-diastolic forward flow (EDFF) in patients with repaired tetralogy of Fallot (rTOF) is not fully understood, with conflicting reports regarding its associations with pulmonary regurgitation (PR), right ventricular (RV) size and function, and so-called restrictive RV physiology. To examine these associations, we retrospectively analyzed 399 patients with rTOF who had contemporaneous echocardiography (Echo) and cardiovascular magnetic resonance (CMR) studies...
March 7, 2018: American Journal of Cardiology
https://www.readbyqxmd.com/read/29677683/application-of-solid-phase-microextraction-followed-by-liquid-chromatography-mass-spectrometry-in-the-determination-of-antibiotic-drugs-and-their-metabolites-in-human-whole-blood-and-tissue-samples
#14
Malgorzata Szultka-Mlynska, Pawel Pomastowski, Boguslaw Buszewski
A sensitive, rapid and specific analytical method using high performance liquid chromatography coupled with mass spectrometry (HPLC-QqQ-MS) was developed to determine selected antibiotic drugs and their metabolites (amoxicillin, cefotaxime, ciprofloxacin, clindamycin and metronidazole; amoxycilloic acid, 4-hydroxyphenyl glycyl amoxicillin, desacetyl cefotaxime, 3-desacetyl cefotaxime lactone, ciprofloxacin N-oxide, N-demethylclindamycin, clindamycin sulfoxide, and hydroxy metronidazole) in human whole blood and vascularized tissue after single oral administration...
April 12, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29677109/antiproliferative-and-antioxidant-activities-of-two-extracts-of-the-plant-species-euphorbia-dendroides-l
#15
Agena Ghout, Amar Zellagui, Noureddine Gherraf, Ibrahim Demirtas, Yaglioglu Ayse Sahin, Meriem Boukhenaf, Mesbah Lahouel, Gema Nieto, Salah Akkal
Background: These days, the desire for naturally occurring antioxidants has significantly increased, especially for use in foodstuffs, cosmetics, and pharmaceutical products, to replace synthetic antioxidants that are regularly constrained due to their carcinogenicity. Methods : The study in hand aimed to appraise the antioxidant effect of two Euphorbia dendroides extracts using reducing power, anti-peroxidation, and DPPH (1,1 Diphenyl 2 Pycril Hydrazil) scavenging essays, in addition to the anticancer activity against two tumor cell lines, namely C6 (rat brain tumor)cells, and Hela (human uterus carcinoma)cell lines...
April 20, 2018: Medicines (Basel, Switzerland)
https://www.readbyqxmd.com/read/29676129/-rapidly-identify-oligosaccharides-in-morinda-officinalis-by-uplc-q-tof-mse
#16
Qing-Xiu Hao, Li-Ping Kang, Shou-Dong Zhu, Yi Yu, Ming-Hua Hu, Fang-Li Ma, Jie Zhou, Lan-Ping Guo
In this paper, an approach was applied for separation and identification of oligosaccharides in Morinda officinalis How by Ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) with collision energy. The separation was carried out on an ACQUITY UPLC BEH Amide C₁₈(2.1mm×100 mm,1.7 μm) with gradient elution using acetonitrile(A) and water(B) containing 0.1% ammonia as mobile phase at a flow rate of 0.2 mL·min⁻¹. The column temperature was maintained at 40 °C...
March 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29676128/-quality-assessment-of-commercial-rosemary-based-on-liquid-gas-chromatography-and-mass-spectrography-with-multivariate-statistical-analysis
#17
Ji-Dan Zhang, Wei Guo, Yan Tang, Ai-Juan Shao, Bin Yang
A GC-MS fingerprint of rosemary oil and an UPLC fingerprint of non-volatile compounds of rosemary were established. Sixty-three kinds of volatile compounds and thirty-eight kinds of non-volatile compounds were identified tentatively using GC-MS and UPLC-Q-TOF-MS, respectively, and most of them are flavonoids, oxygenated monoterpenes, and diterpenoids, etc. According to multivariate data analysis, chemical differences existed among commercial rosemary samples, for example, the essential oil of imported rosemary sample belongs to Morocco/Tunisian type and the one of domestic sample belongs to Spanish type...
March 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29676080/-quality-evaluation-of-medicinal-herb-decoction-forsythiae-fructus
#18
Jiao Xu, Jung Chao, Yun-Tao Dai, Chao Zhu, Qi Li, Tomoda Takehisa, Dan-Dan Wang, Shi-Lin Chen
To establish the quality control methods for the standard decoction of Forsythiae Fructus. Twelve batches of representative Forsythiae Fructus were collected to prepare standard decoction of Forsythiae Fructus, and then the parameters such as extraction ratio, transfer rate of the index components and pH value of the solution were calculated to evaluate the stability of the process. The simultaneous determination method of target components and fingerprint method were established, and ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) was used to identify the main common peaks in the fingerprint to clarify the main chemical constituents in the decoction...
March 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29676079/-quality-evaluation-of-rhei-radix-et-rhizoma-decoction
#19
Man-Jia Zhao, Jung Chao, Yun-Tao Dai, Shi-Lin Chen, Qi Li, Zi-Quan Fan, Dan-Dan Wang, Takehisa Tomoda
Decoction of single medicinal herb is a reference for the standardization of different dosage forms of Chinese medicine and it provides a new direction for solving the problems existing in the quality of Chinese medicinal granules such as no uniform dosage form and no clear quality standard. In this paper, the quality evaluation method of standard decoction of rhubarb was established to provide reference for the quality control of common dosage forms such as clinical decoction and formula granule. 10 batches of representative Rhei Radix et Rhizoma were collected to establish UPLC fingerprints were established...
March 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29675779/antibiotic-resistance-and-molecular-characteristics-of-staphylococcus-aureus-isolated-from-backyard-raised-pigs-and-pig-workers
#20
Asabe Halimat Momoh, Jacob K P Kwaga, Mohammed Bello, Anthony K B Sackey, Anders Rhod Larsen
Staphylococcus aureus is a commensal and pathogenic bacterium with impact on public health and livestock industry. The study investigated nasal carriage, antibiotic resistance, and molecular characterization of S. aureus in pigs and pig workers. Nasal swabs from 300 backyard-raised pigs and 101 pig workers were used for the study. Resulting isolates were confirmed using MALDI-TOF MS, tested for antibiotic resistance, and three different multiplex PCRs were used to detect enterotoxin, mecA, spaA, scn, and pvl genes...
April 19, 2018: Tropical Animal Health and Production
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