keyword
MENU ▼
Read by QxMD icon Read
search

TOF

keyword
https://www.readbyqxmd.com/read/28822947/cell-metabolomics-reveals-the-neurotoxicity-mechanism-of-cadmium-in-pc12-cells
#1
Li Zong, Junpeng Xing, Shu Liu, Zhiqiang Liu, Fengrui Song
The heavy metals such as cadmium (Cd) can induce neurotoxicity. Extensive studies about the effects of Cd on human health have been reported, however, a systematic investigation on the molecular mechanisms of the effects of Cd on central nervous system is still needed. In this paper, the neuronal PC-12 cells were treated with a series of concentrations of CdCl2 for 48h. Then the cytotoxicity was evaluated by MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay. The IC15 value (15% inhibiting concentration) was selected for further mechanism studies...
August 17, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28822909/variation-in-basal-body-localisation-and-targeting-of-trypanosome-rp2-and-for20-proteins
#2
Jane Harmer, Xin Qi, Gabriella Toniolo, Aysha Patel, Hannah Shaw, Fiona E Benson, Michael L Ginger, Paul G McKean
TOF-LisH-PLL motifs define FOP family proteins; some members are involved in flagellum assembly. The critical role of FOP family protein FOR20 is poorly understood. Here, we report relative localisations of the four FOP family proteins in parasitic Trypanosoma brucei: TbRP2, TbOFD1 and TbFOP/FOP1-like are mature basal body proteins whereas TbFOR20 is present on pro- and mature basal bodies - on the latter it localises distal to TbRP2. We discuss how the data, together with published work for another protist Giardia intestinalis, informs on likely FOR20 function...
July 13, 2017: Protist
https://www.readbyqxmd.com/read/28822865/formation-and-morphogenesis-of-a-cuttlebone-s-aragonite-biomineral-structures-for-the-common-cuttlefish-sepia-officinalis-on-the-nanoscale-revisited
#3
Vida Čadež, Srečo D Škapin, Adrijana Leonardi, Igor Križaj, Saša Kazazić, Branka Salopek-Sondi, Ivan Sondi
This study describes and examines the structural and morphological properties of the hierarchically organized, aragonite cuttlebone forms for the common cuttlefish (Sepia officinalis, L.), including its main structural parts, the dorsal shield, and the chambers. Specifically, it complements the mechanism for the self-organized formation of aragonite, identifies the presence, and determines the role of soluble organic matrix (SOM) proteins in the morphogenesis of the cuttlebone's biomineral structures on the nanoscale...
August 10, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28822261/revelation-of-susceptibility-differences-due-to-hg-ii-accumulation-in-streptococcus-pyogenes-against-cx-agnps-and-cefixime-by-atomic-force-microscopy
#4
Wasia Rasheed, Muhammad Raza Shah, Samina Perveen, Shakil Ahmed, Sami Uzzaman
Solution based method for the formation of chemically modified silver nanoparticles (CX-AgNPs) using Cefixime as stabilizing and reducing agent was developed. The CX-AgNPs were characterized by AFM, UV-visible, FT-IR and MALDI-TOF MS. Bactericidal efficiency of CX-AgNPs and Cefixime against Streptococcus pyogenes was evaluated. Afterwards, susceptibility differences of Streptococcus pyogenes due to accumulation of Hg(II) against CX-AgNPs and Cefixime were estimated and validated through Atomic force microscopy...
August 16, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28822196/-metabolic-fingerprint-analysis-of-raw264-7-inflammatory-cell-model-by-using-uplc-q-tof-ms
#5
Shan-Shan Gao, Hui-Qing Guo, Ze-Kun Zhang, Guang-Can Bai, Xiao-Yan Gao, Chang-Hua Ma
In order to reveal the properties of polar metabolome in inflammatory cells, we selected LPS-induced RAW264.7 inflammatory cell models as the carrier for the research of metabolic fingerprint analysis. In this study, an ultra performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF/MS)-based metabolomics protocol was optimized for the extraction of polar metabolites from RAW264.7 cell line. Then orthogonal partial least squares discriminant analysis (OPLS-DA) was used to process the metabolic data, and finally, a total of 17 metabolites were selected and identified...
June 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28822163/-mechanism-of-treatment-effect-of-huanglian-huangqin-herb-pairs-on-cerebral-ischemia-rats-based-on-metabolomic-approach
#6
Hui-Ting Cao, Hua-Xu Zhu, Qi-Chun Zhang, Li-Wei Guo
The metabolic effect of Huanglian-Huangqin herb pairs on cerebral ischemia rats was studied by using metabolomic method. The rat model of ischemia reperfusion injury induced by introduction of transient middle cerebral artery occlusion (MCAO) followed by reperfusion. Ultra high performance liquid chromatography-series four pole time of flight mass spectrometry method(UPLC-Q-TOF/MS), Markerlynx software, and principal component analysis and partial least-squares discriminant analysis were used to analyze the different endogenous metabolites among the urine samples of sham rats, cerebral ischemia model rats, Huanglian groups (HL), Huangqin groups (HQ) and Huanglian-Huangqin herb pairs groups (LQ) was achieved, combined with accurate information about the endogenous metabolites level and secondary fragment ions, retrieval and identification of possible biological markers, metabolic pathway which build in MetPA database...
June 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28822158/-fast-identification-of-constituents-of-lagotis-brevituba-by-using-uplc-q-tof-ms-ms-method
#7
Jing Xie, Li Zhang, Jin-Xiang Zeng, Min Li, Juan Wang, Xiong-Xiong Xie, Guo-Yue Zhong, Guang-Ming Luo, Jin-Bin Yuan, Jian Liang
The chemical constituents of Lagotis brevituba were rapidly determined and analyzed by using ultra performance liquid chromatography tandem quadrupole time of flight mass spectrometry (UPLC-Q-TOF-MS/MS) method, providing material basis for the clinical application of L. brevituba. The separation was performed on UPLC YMC-Triart C₁₈ (2.1 mm×100 mm, 1.9 μm) column, with acetonitrile-water containing 0.2% formic acid as mobile phase for gradient elution. The flow rate was 0.4 mL•min-1 gradient elution and column temperature was 40 ℃, the injection volume was 2 μL...
June 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28821745/metabolomics-and-genomics-combine-to-unravel-the-pathway-for-the-presence-of-fragrance-in-rice
#8
Venea Dara Daygon, Mariafe Calingacion, Louise C Forster, James J De Voss, Brett D Schwartz, Ben Ovenden, David E Alonso, Susan R McCouch, Mary J Garson, Melissa A Fitzgerald
Since it was first characterised in 1983, 2-acetyl-1-pyrroline (2AP) has been considered to be the most important aroma compound in rice. In this study, we show four other amine heterocycles: 6-methyl, 5-oxo-2,3,4,5-tetrahydropyridine (6M5OTP), 2-acetylpyrrole, pyrrole and 1-pyrroline, that correlate strongly with the production of 2AP, and are present in consistent proportions in a set of elite aromatic rice varieties from South East Asia and Australia as well as in a collection of recombinant inbred lines (RILs) derived from indica Jasmine-type varieties, Australian long grain varieties (temperate japonica) and Basmati-type rice (Grp V)...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821212/characterization-of-syntrophic-geobacter-communities-using-tof-sims
#9
Wenchao Wei, Yanyan Zhang, Rachel Komorek, Andrew Plymale, Rujia Yu, Bingchen Wang, Zihua Zhu, Fanghua Liu, Xiao-Ying Yu
The aggregation of syntrophic Geobacter metallireducens and Geobacter sulfurreducens is beneficial for enhancing direct interspecies electron transfer (DIET). Although DIET was suspected to occur on the microbial community surface, the surface chemical speciation of such cocultured communities remains unclear. In order to better understand surface interactions related to DIET, the authors characterized a series of samples associated with syntrophic G. metallireducens and G. sulfurreducens using surface sensitive time-of-flight secondary ion mass spectrometry (ToF-SIMS)...
August 18, 2017: Biointerphases
https://www.readbyqxmd.com/read/28820985/human-bone-marrow-as-a-tissue-in-post-mortem-identification-and-determination-of-psychoactive-substances-screening-methodology
#10
Renata Wietecha-Posłuszny, Sofia Lendor, Magdalena Garnysz, Marcin Zawadzki, Paweł Kościelniak
The aim of this study was to apply a new instrumental approach to the analysis of human bone marrow for forensic purposes. A new screening method for the detection of more than 30 psychoactive drugs in bone marrow was developed and applied to case samples. The drugs used in this study belonged to the following groups: benzodiazepines (BZDs, n=16), tricyclic antidepressants (TCAs, n=5), selective serotonin reuptake inhibitors (SSRI, n=4), serotonin-norepinephrine reuptake inhibitors (n=1), anticonvulsants (n=1), imidazopyridines (n=1) and piperazines (n=3)...
August 7, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28820955/integration-of-an-in-situ-maldi-based-high-throughput-screening-process-a-case-study-with-receptor-tyrosine-kinase-c-met
#11
Katrin Beeman, Jens Baumgärtner, Manuel Laubenheimer, Karlheinz Hergesell, Martin Hoffmann, Ulrich Pehl, Frank Fischer, Jan-Carsten Pieck
Mass spectrometry (MS) is known for its label-free detection of substrates and products from a variety of enzyme reactions. Recent hardware improvements have increased interest in the use of matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS for high-throughput drug discovery. Despite interest in this technology, several challenges remain and must be overcome before MALDI-MS can be integrated as an automated "in-line reader" for high-throughput drug discovery. Two such hurdles include in situ sample processing and deposition, as well as integration of MALDI-MS for enzymatic screening assays that usually contain high levels of MS-incompatible components...
August 1, 2017: SLAS Discovery
https://www.readbyqxmd.com/read/28820460/determination-of-sphingosine-1-phosphate-in-human-plasma-using-liquid-chromatography-coupled-with-q-tof-mass-spectrometry
#12
Emmanuel Eroume-A Egom, Ross Fitzgerald, Rebecca Canning, Rebabonye B Pharithi, Colin Murphy, Vincent Maher
Evidence suggests that high-density lipoprotein (HDL) components distinct from cholesterol, such as sphingosine-1-phosphate (S1P), may account for the anti-atherothrombotic effects attributed to this lipoprotein. The current method for the determination of plasma levels of S1P as well as levels associated with HDL particles is still cumbersome an assay method to be worldwide practical. Recently, a simplified protocol based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the sensitive and specific quantification of plasma levels of S1P with good accuracy has been reported...
August 18, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28819389/discovery-of-non-invasive-glycan-biomarkers-for-detection-and-surveillance-of-gastric-cancer
#13
Ruihuan Qin, Junjie Zhao, Wenjun Qin, Zejian Zhang, Ran Zhao, Jing Han, Yupeng Yang, Lixiao Li, Xuefei Wang, Shifang Ren, Yihong Sun, Jianxin Gu
Purpose: Gastric cancer (GC), one of the world's top five most common cancers, is the third leading cause of cancer related death. It is urgent to identify non-invasive biomarkers for GC. The objective of our study was to find out non-invasive biomarkers for early detection and surveillance of GC based on glycomic analysis. Method: Ethyl esterification derivatization combined with MALDI-TOF MS analysis was employed for the comprehensive serum glycomic analysis in order to investigate glycan markers that would indicate the onset and progression of gastric cancer...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/28818772/multipath-ultrasonic-gas-flow-meter-based-on-multiple-reference-waves
#14
Hongliang Zhou, Tao Ji, Ruichen Wang, Xiaocheng Ge, Xiaoyu Tang, Shizhen Tang
Several technologies can be used in ultrasonic gas flow-meters, such as transit-time, Doppler, cross-correlation and etc. In applications, the approach based on measuring transit-time has demonstrated its advantages and become more popular. Among those techniques which can be applied to determine time-of-flight (TOF) of ultrasonic waves, including threshold detection, cross correlation algorithm and other digital signal processing algorithms, cross correlation algorithm has more advantages when the received ultrasonic signal is severely disturbed by the noise...
July 18, 2017: Ultrasonics
https://www.readbyqxmd.com/read/28818726/identification-of-functional-interactome-of-a-key-cell-division-regulatory-protein-ceda-of-e-coli
#15
Pankaj Sharma, Anil Kumar Tomar, Bishwajit Kundu
Cell division is compromised in DnaAcos mutant Escherichia coli cells that results in filamentous cell morphology. This is countered by over-expression of CedA protein that induces cytokinesis and thus, regular cell morphology is regained; however via an unknown mechanism. To understand the process systematically, exact role of CedA should be deciphered. Protein interactions are crucial for functional organization of a cell and their identification helps in revealing exact function(s) of a protein and its binding partners...
August 14, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28818578/can-exercise-training-counteract-doxorubicin-induced-oxidative-damage-of-testis-proteome
#16
José Magalhães, António Ascensão, Ana I Padrão, Inês M Aleixo, Estela Santos-Alves, Sílvia Rocha-Rodrigues, André Ferreira, Luis Korrodi-Gregório, Rui Vitorino, Rita Ferreira, Margarida Fardilha
The use of the chemotherapeutic drug doxorubicin (DOX) is limited by its toxicity in several organs such as testes. So, we analyzed the effect of endurance treadmill exercise training (EX) performed before sub-chronic DOX treatment on sperm count and motility, testes markers of oxidative damage and apoptosis. Tissue profiling of proteins more susceptible to oxidation was made to identify the molecular pathways regulated by oxidative modifications, as nitration and carbonylation. Twenty-four adult male rats were divided into four groups (n=6/group): sedentary saline (SED+SAL), sedentary sub-chronically injected with DOX (2mg-kg-1 per week, during 7 weeks; SED+DOX), 12 weeks trained saline (EX+SAL) and trained treated with DOX (EX+DOX)...
August 14, 2017: Toxicology Letters
https://www.readbyqxmd.com/read/28817985/rapid-characterisation-and-identification-of-compounds-in-saposhnikoviae-radix-by-high-performance-liquid-chromatography-coupled-with-electrospray-ionisation-quadrupole-time-of-flight-mass-spectrometry
#17
Luxiao Chen, Xiangyang Chen, Lei Su, Yanyan Jiang, Bin Liu
Saposhnikoviae Radix (SR), the dried root of Saposhnikovia divaricata (Turcz.) Schischk. (Umbelliferae), is commonly used as a traditional Chinese medicine. In this study, a rapid and accurate method was firstly, developed for the qualitative analysis of SR by high-performance liquid chromatography coupled with electrospray ionisation quadrupole time-of-flight mass spectrometry (HPLC-ESI-Q-TOF-MS/MS). A total of 45 compounds were identified or tentatively characterised, including 13 chromones, 28 coumarins and four others...
August 18, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28817943/-maldi-tof-ms-comparison-of-blood-culture-positive-bottle-pretreatment-kit
#18
Takenori Yamashita
Identification of bacteria of sepsis or bacteremia is a useful result for treatment policy. In recent years, bacterial identification has become possible from blood culture bottles by MALDI‒TOF, but it is not as accurate as bacterial identification from agar colonies. Blood culture pretreatment kit (MALDI Sepcityper Kit) is currently on sale from Bruker. However, the current situation has not reached good accuracy. This time, a new blood culture pretreatment kit appeared, so I studied. Up to now, the blood culture pretreatment kit was only MALDI Sepcityper Kit using enzyme digestion method...
August 15, 2017: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://www.readbyqxmd.com/read/28817629/maldi-tof-ms-identification-of-anopheles-gambiae-giles-blood-meal-crushed-on-whatman-filter-papers
#19
Sirama Niare, Lionel Almeras, Fatalmoudou Tandina, Amina Yssouf, Affane Bacar, Ali Toilibou, Ogobara Doumbo, Didier Raoult, Philippe Parola
BACKGROUND: Identification of the source of mosquito blood meals is an important component for disease control and surveillance. Recently, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiling has emerged as an effective tool for mosquito blood meal identification, using the abdomens of freshly engorged mosquitoes. In the field, mosquito abdomens are crushed on Whatman filter papers to determine the host feeding patterns by identifying the origin of their blood meals...
2017: PloS One
https://www.readbyqxmd.com/read/28816406/phosphinous-acid-assisted-hydration-of-nitriles-understanding-the-controversial-reactivity-of-osmium-and-ruthenium-catalysts
#20
Rebeca González-Fernández, Pascale Crochet, Victorio Cadierno, M Isabel Menéndez, Ramón López
The synthesis and catalytic behaviour of the osmium(II) complexes [OsCl2(6-p-cymene)(PR2OH)] [R = Me (2a), Ph (2b), OMe (2c), OPh (2d)] in nitrile hydration reactions is presented. Among them, the best catalytic results were obtained with the phosphinous acid derivative [OsCl2(6-p-cymene)(PMe2OH)] (2a), which selectively provided the desired primary amides in excellent yields and short times at 80 ºC, employing directly water as solvent, and without the assistance of any basic additive (TOF values up to 200 h-1)...
August 17, 2017: Chemistry: a European Journal
keyword
keyword
112268
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"