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lumbar degeneration in humans

Demissew Shenegelegn Mern, Anja Tschugg, Sebastian Hartmann, Claudius Thomé
Inhibition of intervertebral disc (IVD) degeneration, which is often accompanied by painful inflammatory and immunopathological processes, is challenging. Current IVD gene therapeutic approaches are based on adenoviral gene delivery systems, which are limited by immune reactions to their viral proteins. Their applications in IVDs near to sensitive neural structure could provoke toxicity and immunological side-effects with neurological deficits. Self-complementary adeno-associated virus (scAAV) vectors, which do not express any viral gene and are not linked with any known disease in humans, are attractive therapeutic gene delivery vectors in degenerative IVDs...
2017: PloS One
S Rajasekaran, Chitraa Tangavel, Siddharth N Aiyer, Sharon Miracle Nayagam, M Raveendran, Naveen Luke Demonte, Pramela Subbaiah, Rishi Kanna, Ajoy Prasad Shetty, K Dharmalingam
STUDY DESIGN: Proteomic and 16S rDNA analysis of disc tissues obtained in vivo. OBJECTIVE: To address the controversy of infection as an aetiology for disc disorders through protein profiling. There is raging controversy over the presence of bacteria in human lumbar discs in vivo, and if they represent contamination or infection. Proteomics can provide valuable insight by identifying proteins signifying bacterial presence and, also host defence response proteins (HDRPs), which will confirm infection...
February 6, 2017: European Spine Journal
U N Ramírez-Jarquín, F Rojas, B van Zundert, R Tapia
Amyotrophic lateral sclerosis is a fatal neurodegenerative disease and studies in vitro show that motoneuron degeneration is triggered by non-cell-autonomous mechanisms. However, whether soluble toxic factor(s) released by mutant superoxide dismutase 1 (SOD1) expressing astrocytes induces death of motoneurons and leads to motor dysfunction in vivo is not known. To directly test this, healthy adult rats were treated with conditioned media derived from primary mouse astrocytes (ACM) that express human (h) SOD1(G93A) (ACM-hG93A) via chronic osmotic pump infusion in the lumbar spinal cord...
January 27, 2017: Journal of Cellular Physiology
Krzysztof A Tomaszewski, Brandon Michael Henry, Tomasz Gładysz, Roman Głowacki, Jerzy A Walocha, Romana Tomaszewska
BACKGROUND CONTEXT: Vertebral joints consist of intervertebral discs (IVDs) and cartilaginous end plates (EP) that lie superiorly and inferiorly to the IVDs and separate them from the adjacent vertebral bodies. With aging, both IVDs and EPs undergo degeneration. The Histologic Degeneration Score (HDS) is a grading system that microscopically evaluates the degree of degeneration in lumbar discs and predicts it with high accuracy basing on several histological markers of IVD and EP. There is currently a lack of validated histologic grading schemes for cervical spine degeneration...
January 16, 2017: Spine Journal: Official Journal of the North American Spine Society
Min Hyung Lee, Il Sup Kim, Jae Taek Hong, Jae Hoon Sung, Sang Won Lee, Daniel H Kim
OBJECTIVE: Low back pain, caused intervertebral disc degeneration has been treated by thermal annuloplasty procedure, which is a non-surgical treatement. The theoretical backgrounds of the annuloplasty are thermal destruct of nociceptor and denaturization of collagen fiber to induce contraction, to shrink annulus and thus enhancing stability. This study is about temperature and its distribution during thermal annuloplasty using 1414 nm Nd : YAG laser. METHODS: Thermal annuloplasty was performed on fresh human cadaveric lumbar spine with 20 intact intervertebral discs in a 37℃ circulating water bath using newly developed 1414 nm Nd : YAG laser...
November 2016: Journal of Korean Neurosurgical Society
Zuo-Qing Liu, Shan Zhao, Wen-Qin Fu
Autophagy has been shown to antagonize the development of Lumbar disc degeneration (LDD), whereas the molecular regulation of autophagy is unknown. We recently reported a potential role of Insulin-like growth factor 1 receptor (IGF1R)/phosphatidylinositol-3 kinase (PI3k)/Akt signaling in the initiation and progression of LDD. Here, we studied the effects of IGF1R signaling on disc cell autophagy. We showed a correction of activation of IGF1R and disc cell autophagy in the resected discs in LDD patients. In vitro, activation of IGF1R signaling antagonized the decreases in cell viability of human disc cells, HNPSV, through suppression of apoptosis and enhancement of autophagy...
2016: American Journal of Translational Research
Maria Claudia Gonzalez Deniselle, Philippe Liere, Antoine Pianos, Maria Meyer, Fanny Aprahamian, Annie Cambourg, Noelia P Di Giorgio, Michael Schumacher, Alejandro F De Nicola, Rachida Guennoun
The The Wobbler mouse is an animal model for human motoneuron diseases, especially amyotrophic lateral sclerosis (ALS), used in the investigation of both pathology and therapeutic treatment. ALS is a fatal neurodegenerative disease, characterized by the selective and progressive death of motoneurons, leading to progressive paralysis. Previous limited studies have reported steroidal hormone dysregulation in Wobbler mouse and in ALS patients, suggesting endocrine dysfunctions which may be involved in the pathogenesis of the disease...
August 29, 2016: Endocrinology
S E Gullbrand, N R Malhotra, T P Schaer, Z Zawacki, J T Martin, J R Bendigo, A H Milby, G R Dodge, E J Vresilovic, D M Elliott, R L Mauck, L J Smith
OBJECTIVE: The objective of this study was to establish a large animal model that recapitulates the spectrum of intervertebral disc degeneration that occurs in humans and which is suitable for pre-clinical evaluation of a wide range of experimental therapeutics. DESIGN: Degeneration was induced in the lumbar intervertebral discs of large frame goats by either intradiscal injection of chondroitinase ABC (ChABC) over a range of dosages (0.1U, 1U or 5U) or subtotal nucleotomy...
January 2017: Osteoarthritis and Cartilage
Ping-Heng Lan, Zhi-Heng Liu, Yan-Jun Pei, Zhi-Gang Wu, Yang Yu, Yong-Feng Yang, Xu Liu, Lu Che, Chi-Jiao Ma, Yan-Ke Xie, Qing-Jie Hu, Zhong-Yuan Wan, Hai-Qiang Wang
Accumulating evidence indicates noncoding RNAs (ncRNAs) fine-tune gene expression with mysterious machinery. We conducted a combination of mRNA, miRNA, circRNA, LncRNA microarray analyses on 10 adults' lumbar discs. Moreover, we performed additional global exploration on RNA interacting machinery in terms of in silico computational pipeline. Here we show the landscape of RNAs in human lumbar discs. In general, the RNA-abundant landscape comprises 14,635 mRNAs (37.93%), 2,059 miRNAs (5.34%), 18,995 LncRNAs (49...
September 27, 2016: Oncotarget
Jean-François Nisolle, Benoît Bihin, Nathalie Kirschvink, Fabienne Neveu, Peter Clegg, Alexandra Dugdale, Xiaoqing Wang, Jean-Michel Vandeweerd
Ovine models are used to study intervertebral disc (IVD) degeneration. The objective of the current study was to assess the naturally occurring age-related changes of the IVD that can be diagnosed by CT and MRI in the lumbar spine of sheep. We used CT and T2-weighted MR images to score the IVD (L6S1 to L1L2) in 41 sheep (age, 6 mo to 11 y) that were euthanized for reasons not related to musculoskeletal disease. T2 mapping and measurement of T2 time of L6S1 to L2L3 were performed in 22 of the sheep. Degenerative changes manifested as early as 2 y of age and occurred at every IVD level...
2016: Comparative Medicine
Tatsuhiro Wada, Osamu Togao, Chiaki Tokunaga, Ryohei Funatsu, Yasuo Yamashita, Kouji Kobayashi, Yasuhiko Nakamura, Hiroshi Honda
PURPOSE: To evaluate the dependence of saturation pulse power and duration on glycosaminoglycan chemical exchange saturation transfer (gagCEST) imaging and assess the degeneration of human lumbar intervertebral discs (IVDs) using this method. MATERIALS AND METHODS: All images were acquired on a 3T magnetic resonance imaging (MRI) scanner. The CEST effects were measured in the glycosaminoglycan (GAG) phantoms with different concentrations. In the human study, CEST effects were measured in the nucleus pulposus of IVD...
July 20, 2016: Journal of Magnetic Resonance Imaging: JMRI
Zuo-Qing Liu, Wen-Qin Fu, Shan Zhao, Xin Zhao
A potential role of Insulin-like growth factor 1 (IGF1) receptor/phosphatidylinositol-3 kinase (PI3k)/Akt signaling in the initiation and progression of Lumbar disc degeneration (LDD) has been recently reported. However, the regulation of IGF1 receptor (IGF1R) at post-transcriptional levels in the development of LDD remains unknown. Here, we studied the effects of microRNA-4458 on the expression of IGF1R. We examined the IGF1R levels and microRNA-4458 (miR-4458) levels in the resected LDD discs, compared with the traumatized, non-LDD discs...
2016: American Journal of Translational Research
Choi Jong-Il, Lim Dong-Jun, Ha Sung-Kon, Kim Sang-Dae, Kim Se-Hoon
AIM: Arthroplasty maintains the biomechanical features of a healthy disc, decreases the adjacent segment disease rate, and decreases the accelerated degeneration rate of the neighboring discs in traditional fusion procedures. However, there are only a few reports on adjacent disc pressure (DP) and facet strain (FS) after lumbar arthroplasty under a physiologic compressive preload. MATERIAL AND METHODS: Baseline DP and FS measurements were obtained from five intact cadaveric human lumbosacral spines for different modes of motion...
January 25, 2016: Turkish Neurosurgery
Vjekoslav Jelec, Rajka Turner, Miljenko Franić, Anđelko Korušić, Krešimir Rotim
The influence of facet orientation and tropism on the process of spinal degeneration has been extensively studied during the last few decades, but there are still many controversies and conflicting results in this field of research. The biomechanical cause of accelerated degeneration of stabilizing structures in lower lumbar spine lies within the combination of several factors, but two most important ones are compressive load and more coronal facet orientation that offers less resistance against torsional loading...
March 2016: Acta Clinica Croatica
Chris Daly, Peter Ghosh, Graham Jenkin, David Oehme, Tony Goldschlager
Lower back pain is the leading cause of disability worldwide. Discogenic pain secondary to intervertebral disc degeneration is a significant cause of low back pain. Disc degeneration is a complex multifactorial process. Animal models are essential to furthering understanding of the degenerative process and testing potential therapies. The adult human lumbar intervertebral disc is characterized by the loss of notochordal cells, relatively large size, essentially avascular nature, and exposure to biomechanical stresses influenced by bipedalism...
2016: BioMed Research International
Dhara B Amin, Dana Sommerfeld, Isaac M Lawless, Richard M Stanley, Boyin Ding, John J Costi
While the effects of disc degeneration on compression and rotation motions have been studied, there is no data for shear loading. Clinical research has shown that those with low back pain (a potential consequence of degeneration) experience a 75% greater lateral shear force than those without it. Therefore, the aim was to compare the effect of degeneration on spine segment stiffness and phase angle in each of six degree of freedom (6DOF) loading directions. Fourteen intact functional spinal units (FSU) were dissected from human lumbar spines (mean (SD) age 76...
August 2016: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
Jeremy P Morgan, Ashley L Miller, Paul A Thompson, Wilson T Asfora
BACKGROUND: Low back pain and degeneration of the intervertebral disc are an integrated malady that affects millions of Americans. Cage devices used in association with posterior lumbar interbody fusion (PLIF) have been shown to be an effective approach in the treatment of a number of lower spine disorders attributed to degenerative disc disease (DDD). OBJECTIVE: This study was undertaken as part of a U.S. Food and Drug Administration (FDA) Investigational Device Exemption (IDE) study and compares the effectiveness of the Asfora Bullet Cage System (ABCS) to successfully fuse vertebra at one or two levels between L2 and S1 in patients with DDD to an FDA approved comparison device, the Medtronic-Sofamor Danek Inter Fix Threaded Fusion Device (MSDIFD)...
April 2016: South Dakota Medicine: the Journal of the South Dakota State Medical Association
Kai Zhang, Wei Sun, Xin-Yu Liu, Chang-Qing Zhao, Hua Li, Xiao-Jiang Sun, Xie You-Zhuan, Wei Ding, Jie Zhao
STUDY DESIGN: Histological, immunohistochemical, and ELISA analysis of the human ligamentum flavum (LF). OBJECTIVE: The purpose of this study was to determine the level of expression of LPA in the LF from LSS patients and to analyze the relationship among LPA, LPA receptors (LPARs) and LF hypertrophy. SUMMARY OF BACKGROUND DATA: Ligamentum flavum (LF) hypertrophy and fibrosis are important causes of Lumbar spinal stenosis (LSS). Lysophosphatidic acid (LPA) is involved in the fibrotic process in multiple organ systems...
June 3, 2016: Clinical Spine Surgery
Nilsson Holguin
No abstract text is available yet for this article.
August 2016: Clinical Orthopaedics and related Research
Yan Li, Dino Samartzis, Desmond D Campbell, Stacey S Cherny, Kenneth M C Cheung, Keith D K Luk, Jaro Karppinen, Youqiang Song, Kathryn S Cheah, Danny Chan, Pak C Sham
BACKGROUND CONTEXT: Lumbar disc degeneration (LDD) is a major cause of low back pain, and is a common and disabling condition worldwide. It has been defined and measured by multiple spine magnetic resonance imaging (MRI) features, but the heterogeneity among them has never been fully addressed. PURPOSE: This study examined the intercorrelations, risk factor associations, and single nucleotide polymorphism (SNP) heritabilities of lumbar disc MRI features in a large-scale sample to classify the different intervertebral disc phenotypes associated with LDD...
September 2016: Spine Journal: Official Journal of the North American Spine Society
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