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https://www.readbyqxmd.com/read/28551720/effect-of-paricalcitol-on-pancreatic-oxidative-stress-inflammatory-markers-and-glycemic-status-in-diabetic-rats
#1
T M Ali, B El Esawy, A Elaskary
OBJECTIVES: This study is designed to explore the effect of paricalcitol (vitamin D receptor agonist) on pancreatic oxidative stress, inflammatory markers, and adiponectin and glycemic status in diabetic rats. MATERIALS AND METHODS: Forty Sprague-Dawley male rats aged 10-12 weeks (150-250 g) were used in this study. Type 2 diabetes was developed by providing 4 weeks of high-fat-diet feeding before one shot of streptozotocin injection (40 mg/kg i.p.). Four study groups were designed as normal control rats, diabetic control vehicle-treated, diabetic paricalcitol-treated (0...
May 27, 2017: Irish Journal of Medical Science
https://www.readbyqxmd.com/read/28551320/effects-of-chronic-abdominal-vagal-stimulation-of-small-diameter-neurons-on-brain-metabolism-and-food-intake
#2
Charles-Henri Malbert, Eric Bobillier, Chloé Picq, Jean-Louis Divoux, David Guiraud, Christine Henry
BACKGROUND: Abdominal bilateral vagal stimulation reduces food intake in animals. However, the classical square wave, mA range current generator is poorly effective to evoke action potentials on A∂ and C neurons that represent the majority of vagal neurons at the abdominal level. OBJECTIVE/HYPOTHESIS: METHODS: The current thresholds for pulsons (S2 & S3) and millisecond pulses (S1) required to trigger action potentials were calculated in 5 anaesthetized pigs using single fibre recording...
April 26, 2017: Brain Stimulation
https://www.readbyqxmd.com/read/28551193/drivers-of-choice-for-fluid-milk-versus-plant-based-alternatives-what-are-consumer-perceptions-of-fluid-milk
#3
K S McCarthy, M Parker, A Ameerally, S L Drake, M A Drake
Fluid milk consumption has declined for decades while consumption of nondairy alternatives has increased. A better understanding of why consumers purchase fluid milk or nondairy alternatives is needed to assist increased sales of milk or maintain sales without further decline. The objective of this study was to determine the extrinsic attributes that drive purchase within each product category. The second objective was to determine the personal values behind the purchase of each beverage type to give further understanding why particular attributes are important...
May 24, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28550720/development-and-validation-of-an-uplc-uv-method-for-determination-of-a-novel-triazine-coccidiostat-ethanamizuril-and-its-metabolite-m3-in-chicken-tissues
#4
Xiao Zhao, Youfa Xu, Lifang Zhang, Chunmei Wang, Chunna Guo, Chenzhong Fei, Keyu Zhang, Xiaoyang Wang, Yingchun Liu, Mi Wang, Wenli Zheng, Feiqun Xue
Ethanamizuril (EZL) is a novel anti-coccidial triazine synthesized by the Shanghai Veterinary Research Institute of the Chinese Academy of Agricultural Sciences. As a novel coccidiostat, it is essential to study its residue in food animal tissues. In this study, a reliable UPLC method for the determination of ethanamizuril (EZL) and one of its metabolites (M3) in chicken muscle, skin and fat, liver, and kidney samples was developed. Analytes were extracted using acetonitrile and 10% sodium carbonate solution, defatted by n-hexane, and further purified by using an Oasis MCX cartridge...
May 11, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28550437/hyperoxaluria-in-a-model-of-mini-gastric-bypass-surgery-in-rats
#5
Milene S Ormanji, Fernando Korkes, Renata Meca, Crysthiane S R A Ishiy, Gustavo H C Finotti, Renato R N Ferraz, Ita P Heilberg
BACKGROUND: Bariatric surgery is associated with hyperoxaluria hence predisposing to nephrolithiasis. The present study aimed to investigate the underlying mechanisms contributing to increased urinary oxalate in a mini-gastric bypass (MGB) surgery model in rats under different dietary conditions. The expression of intestinal oxalate transporters was also evaluated. METHODS: Male rats underwent MGB (n = 21) or Sham procedure (n = 21) and after recovery were fed a standard or high-fat diet with or without oxalate for 8 weeks...
May 26, 2017: Obesity Surgery
https://www.readbyqxmd.com/read/28549558/the-kv1-3-channel-blocker-vm24-enhances-muscle-glucose-transporter-4-mobilization-but-does-not-reduce-body-weight-gain-in-diet-induced-obese-male-rats
#6
Lorraine Jaimes-Hoy, Georgina B Gurrola, Miguel Cisneros, Patricia Joseph-Bravo, Lourival D Possani, Jean-Louis Charli
AIMS: Voltage-gated potassium channels 1.3 (Kv1.3) can be targeted to reduce diet-induced obesity and insulin resistance in mice. Since species-specific differences in Kv1.3 expression and pharmacology have been observed, we tested the effect of Vm24, a high-affinity specific blocker of Kv1.3 channels from Vaejovis mexicanus smithi, on body weight (BW), glucose tolerance and insulin resistance in diet-induced obese rats. MATERIALS AND METHODS: Young adult male Wistar rats were switched to a high-fat/high-fructose (HFF) diet...
May 23, 2017: Life Sciences
https://www.readbyqxmd.com/read/28549202/polymorphism-in-the-beta-casein-gene-and-analysis-of-milk-characteristicsin-gir-and-guzer%C3%A3-dairy-cattle
#7
A H N Rangel, L G Zaros, T C Lima, L H F Borba, L P Novaes, L F M Mota, M S Silva
Several human health problems have been related to the allergenic constitution of bovine milk due to the body's immune reaction to milk proteins. It is necessary find solutions to minimize the occurrence of such reactions, given the importance of milk as a source of animal protein. The aim of this study was to evaluate the allelic frequency of the CSN2 gene and to evaluate differences in the characteristics of Gir and Guzerá bovine milk. One hundred and fifty-six cows were used (68 Gir and 88 Guzerá) from the Felipe Camarão Experimental Station herd of the Agricultural Research Corporation of RN (EMPARN)...
May 25, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28546638/effects-of-obesity-on-liver-cytochromes-p450-in-various-animal-models
#8
Veronika Tomankova, Pavel Anzenbacher, Eva Anzenbacherova
The prevalence of obesity and other obesity-related diseases is increasing worldwide. Obesity is a disease characterized by increased body weight, or a condition resulting from excessive accumulation of body fat. Due to increased body fat deposits, obesity has also been associated with increased mortality resulting from higher incidence rates of hypertension, diabetes, or various types of cancer, such as breast, colorectal, cervical and prostate cancer. Physiological changes associated with obesity are likely to result in altered drug biotransformation...
May 25, 2017: Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia
https://www.readbyqxmd.com/read/28545081/perturbed-adipose-tissue-hydrogen-peroxide-metabolism-in-centrally-obese-men-association-with-insulin-resistance
#9
May G Akl, Eman Fawzy, Maha Deif, Ayman Farouk, Amany K Elshorbagy
OBJECTIVE: Although adipose tissue hydrogen peroxide (H2O2) and its metabolizing enzymes have been linked to obesity and insulin resistance in animal studies, this relation remains to be evaluated in humans. METHODS: Non-diabetic men (N = 43, median age, 49 (37, 54 y)) undergoing abdominal surgeries were studied. Participants were classified by body mass index (BMI) into normal-weight (N = 19), or overweight/obese (Ow/Ob; BMI ≥25; N = 24). Centrally obese men were identified by waist-height ratio ≥0...
2017: PloS One
https://www.readbyqxmd.com/read/28544772/ganglioside-gm3-content-in-skeletal-muscles-is-increased-in-type-2-but-decreased-in-type-1-diabetes-rat-models-implications-of-glycosphingolipid-metabolism-in-pathophysiology-of-diabetes
#10
Josko Bozic, Anita Markotic, Vedrana Cikes-Culic, Anela Novak, Josip Andelo Borovac, Hrvoje Vucemilovic, Gorana Trgo, Tina Ticinovic Kurir
BACKGROUND: Ganglioside GM3 is an abundant constituent of the plasma membrane where its accumulation attenuates insulin receptor signaling. Considering the role of skeletal muscles for insulin-stimulated glucose uptake, the aim of this study was to determine the expression of GM3 and its precursors in skeletal muscle of diabetes mellitus type 1 (DM1) and type 2 (DM2) rat models. METHODS: Diabetes was induced by streptozotocin (55 mg/kg for DM1 and 35 mg/kg for DM2) injection to male Sprague-Dawley rats which were fed with normal pellet diet (DM1 type) or high-fat diet (DM2 type)...
May 22, 2017: Journal of Diabetes
https://www.readbyqxmd.com/read/28544542/conditional-macrophage-depletion-increases-inflammation-and-does-not-inhibit-the-development-of-osteoarthritis-in-obese-mafia-mice
#11
Chia-Lung Wu, Jenna McNeill, Kelsey Goon, Dianne Little, Kelly Kimmerling, Janet Huebner, Virginia Kraus, Farshid Guilak
OBJECTIVE: Macrophages are believed to play a critical role in the inflammation associated with the development of osteoarthritis (OA) in obesity. The objective of the study was to investigate whether short-term, systemic depletion of macrophages would mitigate OA following injury in obese mice. METHODS: CSF-1R-GFP(+) Macrophage Fas-Induced Apoptosis (MaFIA) transgenic mice that allow conditional depletion of macrophages were placed on a high-fat diet and underwent surgery to induce knee OA...
May 23, 2017: Arthritis & Rheumatology
https://www.readbyqxmd.com/read/28543984/cycle-affects-imidacloprid-efficiency-by-mediating-cytochrome-p450-expression-in-the-brown-planthopper-nilaparvata-lugens
#12
K Kang, P Yang, R Pang, L Yue, W Zhang
Circadian clocks influence most behaviours and physiological activities in animals, including daily fluctuations in metabolism. However, how the clock gene cycle influences insects' responses to pesticides has rarely been reported. Here, we provide evidence that cycle affects imidacloprid efficacy by mediating the expression of cytochrome P450 genes in the brown planthopper (BPH) Nilaparvata lugens, a serious insect pest of rice. Survival bioassays showed that the susceptibility of BPH adults to imidacloprid differed significantly between the two time points tested [Zeitgeber Time 8 (ZT8) and ZT4]...
May 23, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/28542922/study-of-retinal-neurodegeneration-and-maculopathy-in-diabetic-meriones-shawi-a-particular-animal-model-with-human-like-macula
#13
Imane Hammoum, Maha Benlarbi, Ahmed Dellaa, Klaudia Szabó, Bulcsú Dékány, Dávid Csaba, Zsuzsanna Almási, Rozina I Hajdú, Rached Azaiz, Ridha Charefeddine, Ákos Lukáts, Rafika Benchouacha-Chekir
The purpose of this work was to evaluate a potentially useful animal model, Meriones shawi (M.sh) - developing metabolic X syndrome, diabetes and possessing a visual streak similar to human macula - in the study of diabetic retinopathy and diabetic macular edema (DME). Type 2 diabetes (T2D) was induced by high fat diet administration in M.sh. Body weights, blood glucose levels were monitored throughout the study. Diabetic retinal histopathology was evaluated 3 and 7 months after diabetes induction. Retinal thickness was measured, retinal cell types were labeled by immunohistochemistry and the number of stained elements were quantified...
May 20, 2017: Journal of Comparative Neurology
https://www.readbyqxmd.com/read/28542544/development-and-characterization-of-an-experimental-model-of-diet-induced-metabolic-syndrome-in-rabbit
#14
Oscar Julián Arias-Mutis, Vannina G Marrachelli, Amparo Ruiz-Saurí, Antonio Alberola, Jose Manuel Morales, Luis Such-Miquel, Daniel Monleon, Francisco J Chorro, Luis Such, Manuel Zarzoso
Metabolic syndrome (MetS) has become one of the main concerns for public health because of its link to cardiovascular disease. Murine models have been used to study the effect of MetS on the cardiovascular system, but they have limitations for studying cardiac electrophysiology. In contrast, the rabbit cardiac electrophysiology is similar to human, but a detailed characterization of the different components of MetS in this animal is still needed. Our objective was to develop and characterize a diet-induced experimental model of MetS that allows the study of cardiovascular remodeling and arrhythmogenesis...
2017: PloS One
https://www.readbyqxmd.com/read/28542485/a-link-between-thrifty-phenotype-and-maternal-care-across-two-generations-of-intercrossed-mice
#15
Bruno Sauce, Carolina P Goes, Isabela Forti, Bruno Gabriel O do Monte, Isabela M Watanabe, Joao Cunha, Andrea C Peripato
Maternal effects are causal influences from mother to offspring beyond genetic information, and have lifelong consequences for multiple traits. Previously, we reported that mice whose mothers did not nurse properly had low birth weight followed by rapid fat accumulation and disturbed development of some organs. That pattern resembles metabolic syndromes known collectively as the thrifty phenotype, which is believed to be an adaptation to a stressful environment which prepares offspring for reduced nutrient supply...
2017: PloS One
https://www.readbyqxmd.com/read/28542353/identification-of-cow-and-buffalo-milk-based-on-beta-carotene-and-vitamin-a-concentration-using-fluorescence-spectroscopy
#16
Rahat Ullah, Saranjam Khan, Hina Ali, Muhammad Bilal, Muhammad Saleem
The current study presents the application of fluorescence spectroscopy for the identification of cow and buffalo milk based on β-carotene and vitamin-A which is of prime importance from the nutritional point of view. All samples were collected from healthy animals of different breeds at the time of lactation in the vicinity of Islamabad, Pakistan. Cow and buffalo milk shows differences at fluorescence emission appeared at band position 382 nm, 440 nm, 505 nm and 525 nm both in classical geometry (right angle) setup as well as front face fluorescence setup...
2017: PloS One
https://www.readbyqxmd.com/read/28540734/effects-of-dietary-fat-on-the-saturated-and-monounsaturated-fatty-acid-metabolism-in-growing-pigs
#17
Stanisława Raj, Grzegorz Skiba, Monika Sobol, Barbara Pastuszewska
The effect of dietary fats differing in fatty acid (FA) composition on the metabolism of saturated FA (SFA) and monounsaturated FA (MUFA) in growing pigs was investigated. The deposition of FA in the body and the fate of individual dietary FA were assessed after slaughter. Gilts with an initial body weight (BW) of 60 kg were used as experimental animals. Six pigs were slaughtered at 60 kg BW, while further 18 pigs received three isoenergetic and isonitrogen experimental diets containing linseed oil, rapeseed oil or beef tallow at 50 g/kg diet until they reached 105 kg (six pigs per group)...
May 25, 2017: Archives of Animal Nutrition
https://www.readbyqxmd.com/read/28536524/mitotherapy-for-fatty-liver-by-intravenous-administration-of-exogenous-mitochondria-in-male-mice
#18
Ailing Fu, Xianxun Shi, Huajing Zhang, Bin Fu
Mitochondrial dysfunction is a major and common mechanism in developing non-alcoholic fatty liver disease (NAFLD). Replacement of dysfunctional mitochondria by functional exogenous mitochondria may attenuate intrahepatic excessive lipid and recover hepatocyte function. However, no data shows that mitochondria can be systemically administrated to animals to date. Here we suggest that mitochondria isolated from hepatoma cells are used as a mitotherapy agent to treat mouse fatty liver induced by high-fat diets...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28534852/effects-of-ketogenic-diets-on-cardiovascular-risk-factors-evidence-from-animal-and-human-studies
#19
REVIEW
Christophe Kosinski, François R Jornayvaz
The treatment of obesity and cardiovascular diseases is one of the most difficult and important challenges nowadays. Weight loss is frequently offered as a therapy and is aimed at improving some of the components of the metabolic syndrome. Among various diets, ketogenic diets, which are very low in carbohydrates and usually high in fats and/or proteins, have gained in popularity. Results regarding the impact of such diets on cardiovascular risk factors are controversial, both in animals and humans, but some improvements notably in obesity and type 2 diabetes have been described...
May 19, 2017: Nutrients
https://www.readbyqxmd.com/read/28533262/acute-and-chronic-changes-in-rat-soleus-muscle-after-high-fat-high-sucrose-diet
#20
Kelsey H Collins, David A Hart, Ian C Smith, Anthony M Issler, Raylene A Reimer, Ruth A Seerattan, Jaqueline L Rios, Walter Herzog
The effects of obesity on different musculoskeletal tissues are not well understood. The glycolytic quadriceps muscles are compromised with obesity, but due to its high oxidative capacity, the soleus muscle may be protected against obesity-induced muscle damage. To determine the time-course relationship between a high-fat/high-sucrose (HFS) metabolic challenge and soleus muscle integrity, defined as intramuscular fat invasion, fibrosis and molecular alterations over six time points. Male Sprague-Dawley rats were fed a HFS diet (n = 64) and killed at serial short-term (3 days, 1 week, 2 weeks, 4 weeks) and long-term (12 weeks, 28 weeks) time points...
May 2017: Physiological Reports
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