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https://www.readbyqxmd.com/read/27901071/staphylococcus-aureus-dependent-septic-arthritis-in-murine-knee-joints-local-immune-response-and-beneficial-effects-of-vaccination
#1
Alessia Corrado, Paolo Donato, Silvia Maccari, Raffaella Cecchi, Tiziana Spadafina, Letizia Arcidiacono, Simona Tavarini, Chiara Sammicheli, Donatello Laera, Andrea Guido Oreste Manetti, Paolo Ruggiero, Bruno Galletti, Sandra Nuti, Ennio De Gregorio, Sylvie Bertholet, Anja Seubert, Fabio Bagnoli, Giuliano Bensi, Emiliano Chiarot
Staphylococcus aureus is the major cause of human septic arthritis and osteomyelitis, which deserve special attention due to their rapid evolution and resistance to treatment. The progression of the disease depends on both bacterial presence in situ and uncontrolled disruptive immune response, which is responsible for chronic disease. Articular and bone infections are often the result of blood bacteremia, with the knees and hips being the most frequently infected joints showing the worst clinical outcome. We report the development of a hematogenous model of septic arthritis in murine knees, which progresses from an acute to a chronic phase, similarly to what occurs in humans...
November 30, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27834064/evaluation-of-bd-max-staph-sr-assay-for-differentiating-between-staphylococcus-aureus-and-coagulase-negative-staphylococci-and-determining-methicillin-resistance-directly-from-positive-blood-cultures
#2
Jaewoong Lee, Yeon Joon Park, Dong Jin Park, Kang Gyun Park, Hae Kyung Lee
BACKGROUND: We evaluated the performance of the BD MAX StaphSR Assay (SR assay; BD, USA) for direct detection of Staphylococcus aureus and methicillin resistance not only in S. aureus but also in coagulase-negative Staphylococci (CNS) from positive blood cultures. METHODS: From 228 blood culture bottles, 103 S. aureus [45 methicillin-resistant S. aureus (MRSA), 55 methicillin-susceptible S. aureus (MSSA), 3 mixed infections (1 MRSA+Enterococcus faecalis, 1 MSSA+MRCNS, 1 MSSA+MSCNS)], and 125 CNS (102 MRCNS, 23 MSCNS) were identified by Vitek 2...
January 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/27790436/surgical-site-infection-by-methicillin-resistant-staphylococcus-aureus-on-decline
#3
Susmita Bhattacharya, Kuhu Pal, Sonia Jain, Shiv Sekhar Chatterjee, Jayashree Konar
INTRODUCTION: Surgical Site Infection (SSI) is the most common healthcare associated infection that could be averted by antibiotics prophylaxis against the probable offending organisms. As Staphylococcus aureus has been playing a substantial role in the aetiology of SSIs, Methicillin Resistant Staphylococcus aureus (MRSA) happens to be a problem while dealing with the postoperative wound infection. AIM: To determine the prevalence of SSI caused by MRSA and the antibiotic sensitivity pattern of MRSA...
September 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27734638/pattern-of-urinary-antibiograms-in-a-tertiary-care-hospital-of-eastern-india
#4
Nandini Chatterjee, Chandan Chatterjee, Sinjan Ghosh, Mainak Mukhopadhyay, Ramkrishna Brahmachari, Kartik Patar
BACKGROUND: Indiscriminate use of antibiotics in urinary tract infections have led to emergence of 'superbugs' worldwide. Periodical review of antibiograms is of utmost importance for optimum patient benefit. OBJECTIVE: To identify the spectrum of organisms responsible for urinary tract infection and evaluate the pattern of antibiotic sensitivity of the organisms. METHODS: Urine samples were collected from all consecutive patients getting admitted in the medicine indoor irrespective of symptomatology...
April 2016: Journal of the Association of Physicians of India
https://www.readbyqxmd.com/read/27720156/identification-of-factors-influencing-teat-dip-efficacy-trial-results-by-meta-analysis
#5
B D Enger, R R White, S C Nickerson, L K Fox
Two meta-analyses were conducted using data from peer-reviewed natural exposure (NE) and experimental challenge (EC) teat dip efficacy trials to identify factors influencing the new intramammary infection (IMI) rate. A NE data set containing 16 studies and an EC data set containing 21 studies were created. New IMI rate was calculated based on the percentage of new quarter infections per month (PNQI/mo) for each observation, in both data sets, and used as the dependent variable for model derivation. A linear, mixed-effects model with a random study effect, weighted by number of quarters eligible for infection, was derived for each data set...
October 5, 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/27638256/short-communication-in-vivo-screening-platform-for-bacteriocins-using-caenorhabditis-elegans-to-control-mastitis-causing-pathogens
#6
S J Son, M R Park, S D Ryu, B E Maburutse, N S Oh, J Park, S Oh, Y Kim
This study aimed to develop an in vivo screening platform using Caenorhabditis elegans to identify a novel bacteriocin for controlling the mastitis-causing pathogen Staphylococcus aureus strain RF122 in dairy cows. Using Bacillus spp. isolated from traditional Korean foods, we developed a direct in vivo screening platform that uses 96-well plates and fluorescence image analysis. We identified a novel bacteriocin produced by Bacillus licheniformis strain 146 (lichenicin 146) with a high in vivo antimicrobial activity using our liquid C...
September 13, 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/27606553/2-5-mafenide-acetate-a-cost-effective-alternative-to-the-5-solution-for-burn-wounds
#7
Ashkan Afshari, Lyly Nguyen, Steven A Kahn, Blair Summitt
Mafenide acetate is an antimicrobial agent used to decrease the bacterial load for burn wounds. The 5% solution is more commonly used yet double the cost of its 2.5% counterpart. This study aims to evaluate outcomes and cost associated with the use of 2.5 vs 5% mafenide acetate formulation in the adult burn population. Adult patients (≥18 years) receiving 2.5% mafenide acetate during an 11-month period between 2014 and 2015, corresponding to a policy change in favor of the use of 2.5% mafenide acetate, were queried...
August 11, 2016: Journal of Burn Care & Research: Official Publication of the American Burn Association
https://www.readbyqxmd.com/read/27592437/investigation-of-the-antibiotic-resistance-and-biofilm-forming-ability-of-staphylococcus-aureus-from-subclinical-bovine-mastitis-cases
#8
Özkan Aslantaş, Cemil Demir
A total of 112 Staphylococcus aureus isolates obtained from subclinical bovine mastitis cases were examined for antibiotic susceptibility and biofilm-forming ability as well as genes responsible for antibiotic resistance, biofilm-forming ability, and adhesin. Antimicrobial susceptibility of the isolates were determined by disk diffusion method. Biofilm forming ability of the isolates were investigated by Congo red agar method, standard tube method, and microplate method. The genes responsible for antibiotic resistance, biofilm-forming ability, and adhesion were examined by PCR...
August 31, 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/27504549/species-identification-and-antibiotic-susceptibilities-of-coagulase-negative-staphylococci-isolated-from-urinary-tract-infection-specimens
#9
Asra Hashmi, Farhan Essa Abdullah, Nihal Essa Abdullah, Shahana Urooj Kazmi
OBJECTIVE: To determine the incidence of Coagulase- negative S. aureusin urinary tract infections and sensitivities of these isolates to antimicrobial agents. STUDY DESIGN: Cohort study. PLACE AND DURATION OF STUDY: Dr. Essa Laboratory and Immunology and Infectious Disease Research Laboratory (IIDRL), Microbiology Department, University of Karachi, from January 2009 to January 2010. METHODOLOGY: Urine specimens, suggestive of urinary tract infection (UTI), were identified...
July 2016: Journal of the College of Physicians and Surgeons—Pakistan: JCPSP
https://www.readbyqxmd.com/read/27495844/epidemiologic-and-demographic-attributes-of-primary-spondylodiscitis-in-a-middle-eastern-population-sample
#10
K Venugopal Menon, Tamer Malak Moawad Sorour
BACKGROUND: The prevalence and characteristics of spontaneous spondylodiscitis in the Middle East is unknown. There seems to be an emerging dominance of pyogenic infections over Tuberculosis and Brucellosis. METHODS: Retrospective observational study based on electronic case records of 4 years from a tertiary reference facility in Oman. Case records, microbiology reports, blood reports, imaging studies, histopathology and surgical reports of all spinal infection cases were studied...
August 2, 2016: World Neurosurgery
https://www.readbyqxmd.com/read/27436387/correlation-of-mupirocin-resistance-with-biofilm-production-in-methicillin-resistant-staphylococcus-aureus-from-surgical-site-infections-in-a-tertiary-centre-egypt
#11
Ghada I Barakat, Yasmin M Nabil
The aim of this study was to detect mupirocin-resistant isolates from pus/wound swabs taken postoperatively in a tertiary centre in Egypt and to determine their ability to form biofilm in order to establish its correlation with mupirocin resistance. This was a prospective study including 513pus/wound swabs from patients suffering from postoperative surgical site infections over the period July 2013-January 2015. Samples were cultured and isolates were identified by coagulase activity, DNase test, mannitol fermentation by mannitol salt agar followed by API Staph 32...
March 2016: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/27358615/a-study-of-asymptomatic-bacteriuria-in-egyptian-school-going-children
#12
Ahmed Mohammed, Magid Abdelfattah, Ayman Ibraheem, Ahmad Younes
BACKGROUND: Urinary tract infections (UTI) are a common and important clinical problem in childhood. Upper urinary tract infections (i.e., acute pyelonephritis) may lead to renal scarring, hypertension, and end-stage renal disease. Despite the presence of simple and reliable methods of preliminary screening of children's urine, urinary tract infection continues to be under diagnosed. OBJECTIVES: The aim of this study was to establish prevalence rates of significant bacteriuria in asymptomatic school children by simple urine tests in comparison to standard urine culture techniques in Giza, Egypt...
March 2016: African Health Sciences
https://www.readbyqxmd.com/read/27214804/use-of-central-venous-catheters-in-children-with-haemophilia-one-haemophilia-treatment-centre-experience
#13
I Warrier, K Baird-Cox, J M Lusher
We report our clinic experience with central venous catheters (CVCs) in 23 children with haemophilia, who, in total, had 35 catheters. Seventeen of the 23 children had Broviac catheters (external), 6/23 had an implantable device (Port-A-Cath) only, while four had Broviac and Port-A-Cath at different times. The age of the patients at the time of initial catheter placement ranged from 4 months to 18 years; 11 were under age 3 years. Indications for CVC placement included induction of immune tolerance, treatment of HIV-related complications, prophylactic therapy following intracranial haemorrhage, primary prophylaxis and secondary prophylaxis...
July 1997: Haemophilia: the Official Journal of the World Federation of Hemophilia
https://www.readbyqxmd.com/read/27099680/genotyping-of-coa-and-aroa-genes-of-methicillin-resistant-staphylococcus-aureus-strains-isolated-from-nasal-samples-in-western-iran
#14
Parviz Mohajeri, Samira Azizkhani, Abbas Farahani, Baharak Norozi
BACKGROUND: Methicillin-resistant Staphylococcus aureus (MRSA) is a bacterial pathogen frequently isolated in both hospital and community environments. Methicillin-resistant Staphylococcus aureus is considered a major nosocomial pathogen that causes severe morbidity and mortality. OBJECTIVES: The main objective of this study was to determine the genotypes of MRSA strains isolated from the nares of hospitalized and community patients in Kermanshah Hospital, western Iran, by PCR-restriction fragment length polymorphism (PCR-RFLP)...
January 2016: Jundishapur Journal of Microbiology
https://www.readbyqxmd.com/read/27096795/prevalence-and-etiological-agents-of-subclinical-mastitis-at-the-end-of-lactation-in-nine-dairy-herds-in-north-east-poland
#15
M Sztachańska, W Barański, T Janowski, J Pogorzelska, S Zduńczyk
The aim of this study was to evaluate the prevalence and etiological agents of subclinical mastitis at the end of lactation in nine dairy herds in North-East Poland. In total, 387 Polish HF were involved in the study. The diagnosis of mastitis was performed on the basis of clinical examination of the udder, macroscopic evaluation of milk, determination of somatic cell count and bacteriological examination of milk. Subclinical mastitis was found in an average of 36.7% (range from 21.0% to 53.1%) of cows and of 15...
2016: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/27040789/evaluation-of-a-lysostaphin-fusion-protein-as-a-dry-cow-therapy-for-staphylococcus-aureus-mastitis-in-dairy-cattle
#16
K J Hoernig, D M Donovan, P Pithua, F Williams, J R Middleton
This study evaluated the efficacy of a recombinant lysostaphin fused to a protein transduction domain (rLYS-PTD) as a dry-cow therapy for the treatment of experimentally induced chronic, subclinical Staphylococcus aureus mastitis. Twenty-two Holstein dairy cows were experimentally infected with Staph. aureus in a single pair of diagonal mammary quarters approximately 45d before dry off. Staphylococcus aureus-infected mammary quarters of cows were randomly assigned to 1 of 2 treatment groups at dry off: (1) 279mg of rLYS-PTD in 50mL of vehicle (n=11 cows; 22 quarters) or (2) 50mL of vehicle solution (n=11 cows; 22 quarters) by intramammary infusion...
June 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/26958825/antimicrobial-activity-of-extracts-from-macroalgae-ulva-lactuca-against-clinically-important-staphylococci-is-impacted-by-lunar-phase-of-macroalgae-harvest
#17
A M Deveau, Z Miller-Hope, E Lloyd, B S Williams, C Bolduc, J M Meader, F Weiss, K M Burkholder
UNLABELLED: Staphylococcus aureus is a common human bacterial pathogen that causes skin and soft tissue infections. Methicillin-resistant Staph. aureus (MRSA) are increasingly drug-resistant, and thus there is great need for new therapeutics to treat Staph. aureus infections. Attention has focused on potential utility of natural products, such as extracts of marine macroalgae, as a source of novel antimicrobial compounds. The green macroalgae Ulva lactuca produces compounds inhibitory to human pathogens, although the effectiveness of U...
May 2016: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/26873818/clinical-presentation-management-and-outcomes-of-staph-aureus-bacteremia-sab-in-older-adults
#18
Dafna Yahav, Agata Schlesinger, Hila Shaked, Elad Goldberg, Mical Paul, Jihad Bishara, Leonard Leibovici
AIM: The incidence of Staphylococcus aureus bacteremia (SAB) increases with advancing age with higher mortality reported in older adults. We aimed to describe the clinical presentation, management and outcomes of older patients with SAB. METHODS: We analyzed data from a retrospectively collected database including 1692 patients with SAB, and compared 1158 older patients (≥65 years) with 534 younger patients (<65 years) in terms of clinical features, management of infection, and outcomes...
February 12, 2016: Aging Clinical and Experimental Research
https://www.readbyqxmd.com/read/26830736/interaction-between-bovine-associated-coagulase-negative-staphylococci-species-and-strains-and-bovine-mammary-epithelial-cells-reflects-differences-in-ecology-and-epidemiological-behavior
#19
F N Souza, S Piepers, A M M P Della Libera, M B Heinemann, M M O P Cerqueira, S De Vliegher
Bacteria adherence seems to be an essential first stage for the internalization of bacteria into the cytoplasm of the host cell, which is considered an important virulence strategy enabling bacteria to occupy a microenvironment separated from host defense mechanisms. Thus, this study aimed to explore the difference in the capacity of 4 bovine-associated staphylococci species or strains to adhere to and internalize into bovine mammary epithelial cells (MEC). Three different isolates of coagulase-negative staphylococci (CNS) were used: one strain of Staphylococcus fleurettii isolated from sawdust and considered an environmental opportunistic bacterium, and 2 dissimilar Staphylococcus chromogenes isolates, one cultured from a heifer's teat apex (Staph...
April 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/26805990/udder-infections-with-staphylococcus-aureus-streptococcus-dysgalactiae-and-streptococcus-uberis-at-calving-in-dairy-herds-with-suboptimal-udder-health
#20
Å Lundberg, A-K Nyman, A Aspán, S Börjesson, H Ericsson Unnerstad, K Persson Waller
Udder infections with Staphylococcus aureus, Streptococcus dysgalactiae, and Streptococcus uberis are common causes of bovine mastitis. To study these pathogens in early lactation, a 12-mo longitudinal, observational study was carried out in 13 herds with suboptimal udder health. The aims of the study were to investigate the occurrence of these pathogens and to identify if presence of the 3 pathogens, and of genotypes within the pathogens, differed with respect to herd, season, and parity. Quarter milk samples, collected at calving and 4 d in milk (DIM), were cultured for the 3 pathogens...
March 2016: Journal of Dairy Science
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