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NAC AND Mitochondria

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https://www.readbyqxmd.com/read/28176146/centchroman-induces-redox-dependent-apoptosis-and-cell-cycle-arrest-in-human-endometrial-cancer-cells
#1
Hari Shyam, Neetu Singh, Shweta Kaushik, Ramesh Sharma, Anil K Balapure
Centchroman (CC) or Ormeloxifene has been shown to induce apoptosis and cell cycle arrest in various types of cancer cells. This has, however, not been addressed for endometrial cancer cells where its (CC) mechanism of action remains unclear. This study focuses on the basis of antineoplasticity of CC by blocking the targets involved in the cell cycle, survival and apoptosis in endometrial cancer cells. Ishikawa Human Endometrial Cancer Cells were cultured under estrogen deprived medium, exposed to CC and analyzed for proliferation and apoptosis...
February 7, 2017: Apoptosis: An International Journal on Programmed Cell Death
https://www.readbyqxmd.com/read/28130091/nitric-oxide-no-mediated-mitochondrial-damage-plays-a-critical-role-in-t-2-toxin-induced-apoptosis-and-growth-hormone-deficiency-in-rat-anterior-pituitary-gh3-cells
#2
Xianglian Liu, Pu Guo, Aimei Liu, Qinghua Wu, Xijuan Xue, Menghong Dai, Haihong Hao, Wei Qu, Shuyu Xie, Xu Wang, Zonghui Yuan
T-2 toxin, a major compound of trichothecenes, induces cell apoptosis and growth hormone (GH) deficiency and causes considerable growth retardation in animals and human cells. However, the mechanism underlying its growth suppression still remains unclear. Recent studies have suggested that ROS induced cell apoptosis and animal feed intake reduction, but there are limited reports on the role of RNS in T-2 toxin-mediated mitochondrial damage, cell apoptosis and growth retardation. Herein, T-2 toxin-induced GH3 cell damage and apoptosis were tested by MTT assay, LDH leakage and flow cytometry, respectively...
January 24, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28123000/dual-role-of-epidermal-growth-factor-receptor-in-liver-injury-and-regeneration-after-acetaminophen-overdose-in-mice
#3
Bharat Bhushan, Hemantkumar Chavan, Prachi Borude, Yuchao Xie, Kuo Du, Mitchell R McGill, Margitta Lebofsky, Hartmut Jaeschke, Partha Krishnamurthy, Udayan Apte
Epidermal growth factor receptor (EGFR) plays a crucial role in hepatocyte proliferation. Its role in acetaminophen (APAP)-mediated hepatotoxicity and subsequent liver regeneration is completely unknown. Role of EGFR after APAP-overdose in mice was studied using pharmacological inhibition strategy. Rapid, sustained and dose-dependent activation of EGFR was noted after APAP-treatment in mice, which was triggered by glutathione depletion. EGFR-activation was also observed in primary human hepatocytes after APAP-treatment, preceding elevation of toxicity markers...
February 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/28118671/mechanistic-approach-for-toxic-effects-of-bupropion-in-primary-rat-hepatocytes
#4
Elham Ahmadian, Hossein Babaei, Alireza Mohajjel Nayebi, Aziz Eftekhari, Mohammad Ali Eghbal
Bupropion is a widely prescribed antidepressant/smoke cessation drug. However, hepatotoxicity is one of its side effects reported in some recipients. The mechanisms by which bupropion induces hepatotoxicity is not clear yet. This experiment was intended to assess the cytotoxic mechanisms of bupropion toward primary rat hepatocytes. Additionally, the effect of α-tocopherol succinate (ALPHA-TOS) and N-acetyl cysteine (NAC) and mitochondrial permeability transition (MPT) pore sealing agent cyclosporine A (Cs A) on this toxicity was investigated...
January 24, 2017: Drug Research
https://www.readbyqxmd.com/read/28109174/%C3%AE-%C3%AE-nac-cooperates-with-sam37-to-mediate-early-stages-of-mitochondrial-protein-import
#5
J Carlos Ponce-Rojas, M Clara Avendaño-Monsalve, A Roberto Yañez-Falcón, Fabiola Jaimes-Miranda, Erika Garay, Francisco Torres-Quiroz, Alexander DeLuna, Soledad Funes
The mitochondrial proteome is mostly composed of nuclear-encoded proteins. Such polypeptides are synthesized with signals that guide their intracellular transport to the surface of the organelle and later within the different mitochondrial sub-compartments until they reach their functional destination. It has been suggested that NAC - a cytosolic chaperone which recognizes nascent chains on translationally active ribosomes - has a role in the import of nuclear-encoded mitochondrial proteins. However, the molecular mechanisms that regulate the NAC-mediated co-translational import are still not clear...
January 21, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28088644/tert-butyl-hydroperoxide-t-bhp-induced-apoptosis-and-necroptosis-in-endothelial-cells-roles-of-nox4-and-mitochondrion
#6
Wenwen Zhao, Haitao Feng, Wen Sun, Kang Liu, Jin-Jian Lu, Xiuping Chen
Oxidative stress causes endothelial death while underlying mechanisms remain elusive. Herein, the pro-death effect of tert-butyl hydroperoxide (t-BHP) was investigated with low concentration (50μM) of t-BHP (t-BHPL) and high concentration (500μM) of t-BHP (t-BHPH). Both t-BHPL and t-BHPH induced endothelial cell death was determined. T-BHPL induced caspase-dependent apoptosis and reactive oxygen species (ROS) generation, which was inhibited by N-acetyl-L-cysteine (NAC). Furthermore, NADPH oxidase inhibitor diphenyleneiodonium (DPI), NOX4 siRNA, and NOX4 inhibitor GKT137831 reduced t-BHPL-induced ROS generation while mitochondrial respiratory chain inhibitors rotenone (Rot), 2-thenoyltrifluoroacetone (TTFA), and antimycin A (AA) failed to do so...
January 5, 2017: Redox Biology
https://www.readbyqxmd.com/read/28087840/vibrio-cholerae-cholix-toxin-induced-hepg2-cell-death-is-enhanced-by-tumor-necrosis-factor-alpha-through-ros-and-intracellular-signal-regulated-kinases
#7
Kohei Ogura, Yasuhiro Terasaki, Tohru Miyoshi-Akiyama, Mika Terasaki, Joel Moss, Masatoshi Noda, Kinnosuke Yahiro
Cholix toxin (Cholix) from Vibrio cholerae is a potent virulence factor exhibiting ADP-ribosyltransferase activity on eukaryotic elongation factor 2 (eEF2) of host cells, resulting in inhibition of protein synthesis. Administration of Cholix or its homologue Pseudomonas exotoxin A (PEA) to mice causes lethal hepatocyte damage. In this study, we demonstrate cytotoxicity of Cholix on immortalized human hepatocytes in the presence of tumor necrosis factor α (TNF-α), which has been reported to play a fatal role in PEA administered to mice...
January 13, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/28057718/calcium-mediated-oxidative-stress-a-common-mechanism-in-tight-junction-disruption-by-different-types-of-cellular-stress
#8
Ruchika Gangwar, Avtar Meena, Pradeep Shukla, Archana Nagaraja, Piotr Dorniak, Sandeep Pallikuth, Christopher Waters, Anil Sood, Radhakrishna Rao
The role of reactive oxygen species (ROS) in osmotic stress, dextran sulfate sodium (DSS) and cyclic stretch-induced tight junction disruption was investigated in Caco-2 cell monolayers in vitro , and restraint stress-induced barrier dysfunction in mouse colon in vivo Live cell imaging showed that osmotic stress, cyclic stretch and DSS triggered rapid production of ROS in Caco-2 cell monolayers, which was blocked by depletion of intracellular Ca(2+) by BAPTA. Knockdown of CaV1.3 or TRPV6 channels blocked osmotic stress and DSS-induced ROS production and attenuated tight junction disruption and barrier dysfunction...
January 5, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/28057548/the-protection-effects-of-1e-6e-1-7-diphenylhepta-1-6-diene-3-5-dione-a-curcumin-analogue-against-operative-liver-injury-in-rats
#9
Xiaowei Chi, Dan Yu, Peijing Li, Qianfeng Lu, Wenjiao Jiang, Kun Hao
The relationship between the chemistry characteristic and the hepatoprotective effects of (1E,6E)-1,7-diphenylhepta-1,6-diene-3,5-dione (DDD), a curcumin analogue, in operative liver injury rats was investigated to reveal the mechanism of hepatic protection effects of DDD. DDD (1.2-4.8mmol/kg) was administrated 10min before reperfusion phase in hepatic ischemia-reperfusion injury (IRI) rats. DDD (4.8mmol/kg) administrated 10min before ischemia and N-acetylcysteine (NAC) (4.8mmol/kg) administrated 10min before reperfusion were included for comparative studies...
January 3, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/27925012/effect-of-nac-on-mouse-gv-oocyte-survival-and-subsequent-embryonic-development-following-vitrfication
#10
S L Yue, Y T Zhang, S W Wang, M Sun, Y C Xing, J Wen, J B Zhou
BACKGROUND: Oocytes that survive cryopreservation may accumulate ROS which are known to bring harmful effects on embryonic development. NAC is an antioxidant which can be a supplement to reduce oxidative stress. However, whether NAC can improve the developmental competence of vitrified GV-oocytes remains unclear. OBJECTIVE: The study was to investigate the effect of NAC on subsequent embryonic developmental competence of mice vitrified GV-oocytes. MATERIALS AND METHODS: This study compared the effects of different concentration of NAC on the cleavage and blastocyst rates of mice vitrified GV-oocytes...
July 2016: Cryo Letters
https://www.readbyqxmd.com/read/27907115/brucella-melitensis-16m-regulates-the-effect-of-air-domain-on-inflammatory-factors-autophagy-and-apoptosis-in-mouse-macrophage-through-the-ros-signaling-pathway
#11
Tiansen Li, Yafang Xu, Laizhen Liu, Meiling Huang, Zhen Wang, Zhixia Tong, Hui Zhang, Fei Guo, Chuangfu Chen
Brucellosis is a highly contagious zoonosis caused by Brucella. Brucella can invade and persist inside host cells, which results in chronic infection. We constructed AIR interference and overexpression lentiviruses to acquire AIR interference, overexpression, and rescue stable expression cell lines. We also established a Brucella melitensis 16M-infected macrophage model, which was treated with either the vehicle control or NAC (ROS scavenger N-acetylcysteine (NAC) for 0, 3, 6, 12, and 24 h. Confocal laser microscopy, transmission electron microscopy, fluorescence quantitative PCR, flow cytometry, ELISA, and Western blot were used to detect inflammation, cell autophagy and apoptosis-related protein expression levels, ROS levels, and the distribution of mitochondria...
2016: PloS One
https://www.readbyqxmd.com/read/27902334/the-ns1-protein-of-avian-influenza-virus-h9n2-induces-oxidative-stress-mediated-chicken-oviduct-epithelial-cells-apoptosis
#12
Xuefeng Qi, Huizhu Zhang, Qiuzhen Wang, Jingyu Wang
The pathogenesis of H9N2 subtype avian influenza virus infection (AIV) in hens is often related to oviduct tissue damage. The viral non-structural NS1 protein is thought to play a key role in regulating the pathogenicity of AIV, but its exact function in this process remains elusive. In this study, the pro-apoptosis effect of H9N2 NS1 protein was examined on chicken oviduct epithelial cells (COECs) and our data indicated that NS1-induced oxidative stress was a contributing factor in apoptosis. Our data indicate that NS1 protein level was correlated with reactive oxygen species (ROS) in COECs transfected with NS1 expression plasmids...
December 2016: Journal of General Virology
https://www.readbyqxmd.com/read/27900412/demethyleneberberine-alleviates-inflammatory-bowel-disease-in-mice-through-regulating-nf-%C3%AE%C2%BAb-signaling-and-t-helper-cell-homeostasis
#13
Ying-Ying Chen, Rui-Yan Li, Mei-Jing Shi, Ya-Xing Zhao, Yan Yan, Xin-Xin Xu, Miao Zhang, Xiao-Tong Zhao, Yu-Bin Zhang
OBJECTIVE: The activation of NF-κB signaling and unbalance of T-helper (Th) cells have been reported to play a key role in the pathogenesis of colitis. Cortex Phellodendri Chinensis (CPC) is commonly used to treat inflammation and diarrhea. Demethyleneberberine (DMB), a component of CPC, was reported to treat alcoholic liver disease as a novel natural mitochondria-targeted antioxidant in our previous study. In this study, we investigated whether DMB could protect against dextran sulfate sodium (DSS)-induced inflammatory colitis in mice by regulation of NF-κB pathway and Th cells homeostatis...
November 29, 2016: Inflammation Research: Official Journal of the European Histamine Research Society ... [et Al.]
https://www.readbyqxmd.com/read/27888799/new-bipyridine-gold-iii-dithiocarbamate-containing-complexes-exerted-a-potent-anticancer-activity-against-cisplatin-resistant-cancer-cells-independent-of-p53-status
#14
Muhammad Altaf, Muhammad Monim-Ul-Mehboob, Abdel-Nasser Kawde, Giuseppe Corona, Roberto Larcher, Marcia Ogasawara, Naike Casagrande, Marta Celegato, Cinzia Borghese, Zahid H Siddik, Donatella Aldinucci, Anvarhusein A Isab
We synthesized, characterized and tested in a panel of cancer cell lines, nine new bipyridine gold(III) dithiocarbamate-containing complexes. In vitro studies demonstrated that compounds 1, 2, 4, 5, 7 and 8 were the most cytotoxic in prostate, breast, ovarian cancer cell lines and in Hodgkin lymphoma cells with IC50 values lower than the reference drug cisplatin. The most active compound 1 was more active than cisplatin in ovarian (A2780cis and 2780CP-16) and breast cancer cisplatin-resistant cells. Compound 1 determined an alteration of the cellular redox homeostasis leading to increased ROS levels, a decrease in the mitochondrial membrane potential, cytochrome-c release from the mitochondria and activation of caspases 9 and 3...
January 3, 2017: Oncotarget
https://www.readbyqxmd.com/read/27888692/mitochondrial-gsh-replenishment-as-a-potential-therapeutic-approach-for-niemann-pick-type-c-disease
#15
Sandra Torres, Nuria Matías, Anna Baulies, Susana Nuñez, Cristina Alarcon-Vila, Laura Martinez, Natalia Nuño, Anna Fernandez, Joan Caballeria, Thierry Levade, Alba Gonzalez-Franquesa, Pablo Garcia-Rovés, Elisa Balboa, Silvana Zanlungo, Gemma Fabrías, Josefina Casas, Carlos Enrich, Carmen Garcia-Ruiz, José C Fernández-Checa
Niemann Pick type C (NPC) disease is a progressive lysosomal storage disorder caused by mutations in genes encoding NPC1/NPC2 proteins, characterized by neurological defects, hepatosplenomegaly and premature death. While the primary biochemical feature of NPC disease is the intracellular accumulation of cholesterol and gangliosides, predominantly in endolysosomes, mitochondrial cholesterol accumulation has also been reported. As accumulation of cholesterol in mitochondria is known to impair the transport of GSH into mitochondria, resulting in mitochondrial GSH (mGSH) depletion, we investigated the impact of mGSH recovery in NPC disease...
November 20, 2016: Redox Biology
https://www.readbyqxmd.com/read/27863415/induction-of-the-mitochondria-mediated-apoptosis-in-human-esophageal-cancer-cells-by-ds2-a-newly-synthetic-diterpenoid-analog-is-regulated-by-bax-and-caused-by-generation-of-reactive-oxygen-species
#16
Yong-Cheng Ma, Yu Ke, Xiaolin Zi, Fei Zhao, Lin Yuan, Ying-Li Zhu, Xia-Xia Fan, Ning-Min Zhao, Qiao-Yan Li, Yu-Hua Qin, Hong-Min Liu
Ent-kaurane diterpene compounds have attracted considerable attention in recent years due to its antitumor, antibacterial, and antiviral activities. However, the clinical development of natural kaurane diterpenes, for example, oridonin for cancer therapy has been hampered by its relatively moderate potency, limited bioavailability. Herein, we report a newly synthetic analog of natural ent-kaurane diterpene, DS2, which exhibits significantly improved activity of antiproliferation against various cancer cell lines relative to oridonin...
November 15, 2016: Oncotarget
https://www.readbyqxmd.com/read/27862231/early-stage-functions-of-mitochondrial-autophagy-and-oxidative-stress-in-acetaminophen-induced-liver-injury
#17
Yan Gao, Shifeng Chu, Zhao Zhang, Wei Zuo, Congyuan Xia, Qidi Ai, Piao Luo, Peng Cao, Naihong Chen
Mitochondria go through frequent cycles of fusion and fission, a process required for mitochondrial quality control by eliminating ROS-damaged mitochondria through mitochondrial autophagy. Acetaminophen (APAP) overdose can cause liver injury in animals and human beings by inducing mitochondrial damage, which need to be further evaluated. The aim of the current study is to assess the changes between oxidative damage and mitophagy in vivo and in vitro, which mimics APAP-induced liver injury (AILI) in humans. Liver damage was monitored by measuring the levels of biochemical indexes...
November 9, 2016: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/27855364/parthenolide-induces-reactive-oxygen-species-mediated-autophagic-cell-death-in-human-osteosarcoma-cells
#18
Chen Yang, Qing Ou Yang, Qing-Jie Kong, Wen Yuan, Yue-Ping Ou Yang
BACKGROUND AND AIM: Osteosarcoma is a devastating tumor of bone, primarily affecting adolescents. Parthenolide, a naturally occurring small molecule that interferes with NF-κB signaling, has recently attracted considerable attention because of its pharmacological action involving anti-cancer effects. However, the mechanism of the cytotoxic effect exerted by parthenolide on tumor cells is not clearly defined today. METHODS: In this study, the effects of parthenolide were evaluated and characterized in human osteosarcoma cancer cell...
2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27843711/iron-overload-induced-death-of-osteoblasts-in-vitro-involvement-of-the-mitochondrial-apoptotic-pathway
#19
Qing Tian, Shilei Wu, Zhipeng Dai, Jingjing Yang, Jin Zheng, Qixin Zheng, Yong Liu
BACKGROUND: Iron overload is recognized as a new pathogenfor osteoporosis. Various studies demonstrated that iron overload could induce apoptosis in osteoblasts and osteoporosis in vivo. However, the exact molecular mechanisms involved in the iron overload-mediated induction of apoptosis in osteoblasts has not been explored. PURPOSE: In this study, we attempted to determine whether the mitochondrial apoptotic pathway is involved in iron-induced osteoblastic cell death and to investigate the beneficial effect of N-acetyl-cysteine (NAC) in iron-induced cytotoxicity...
2016: PeerJ
https://www.readbyqxmd.com/read/27831562/mitochondrial-transfer-of-mesenchymal-stem-cells-effectively-protects-corneal-epithelial-cells-from-mitochondrial-damage
#20
Dan Jiang, Fei Gao, Yuelin Zhang, David Sai Hung Wong, Qing Li, Hung-Fat Tse, Goufeng Xu, Zhendong Yu, Qizhou Lian
Recent studies have demonstrated that mesenchymal stem cells (MSCs) can donate mitochondria to airway epithelial cells and rescue mitochondrial damage in lung injury. We sought to determine whether MSCs could donate mitochondria and protect against oxidative stress-induced mitochondrial dysfunction in the cornea. Co-culturing of MSCs and corneal epithelial cells (CECs) indicated that the efficiency of mitochondrial transfer from MSCs to CECs was enhanced by Rotenone (Rot)-induced oxidative stress. The efficient mitochondrial transfer was associated with increased formation of tunneling nanotubes (TNTs) between MSCs and CECs, tubular connections that allowed direct intercellular communication...
November 10, 2016: Cell Death & Disease
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