Tiantian Tian, Huan Pang, Xinyue Li, Kai Ma, Tianxiang Liu, Jiali Li, Zhixuan Luo, Meng Li, Qiaohong Hou, Huifang Hao, Jianfei Dong, Haiying Du, Xiaomei Liu, Zhiwei Sun, Chao Zhao, Xiuling Song, Minghua Jin
Silica nanoparticles (SiNPs) are widely used in the biomedical field and can enter the central nervous system through the blood-brain barrier, causing damage to hippocampal neurons. However, the specific mechanism remains unclear. In this experiment, HT22 cells were selected as the experimental model in vitro, and the survival rate of cells under the action of SiNPs was detected by MTT method, reactive oxygen species (ROS), lactate dehydrogenase (LDH), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and adenosine triphosphate (ATP) were tested by the kit, the ultrastructure of the cells was observed by transmission electron microscope, membrane potential (MMP), calcium ion (Ca2+ ) and apoptosis rate were measured by flow cytometry, and the expressions of mitochondrial functional protein, mitochondrial dynein, mitochondrial autophagy protein as well as apoptosis related protein were detected by Western blot...
February 5, 2024: Ecotoxicology and Environmental Safety