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Escherichia Coli O157 infection

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https://www.readbyqxmd.com/read/28506793/frequency-and-risk-factors-analysis-of-escherichia-coli-o157-h7-in-bali-cattle
#1
I Wayan Suardana, Dyah Ayu Widiasih, Widagdo Sri Nugroho, Michael Haryadi Wibowo, I Nyoman Suyasa
Cattle are known as the main reservoir of zoonotic agents verocytotoxin-producing Escherichia coli. These bacteria are usually isolated from calves with diarrhea and/or mucus and blood. Tolerance of these agents to the environmental conditions will strengthen of their transmission among livestock. A total of 238 cattle fecal samples from four sub-districts in Badung, Bali were used in this study. Epidemiological data observed include cattle age, sex, cattle rearing system, the source of drinking water, weather, altitude, and type of cage floor, the cleanliness of cage floor, the slope of cage floor, and the level of cattle cleanliness...
May 12, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28505584/development-of-a-gold-nanoparticle-based-universal-oligonucleotide-microarray-for-multiplex-and-low-cost-detection-of-foodborne-pathogens
#2
Xiaoqiang Wang, Sisi Ying, Xiaoguang Wei, Jun Yuan
Bacterial foodborne diseases remain major threats to food safety and public health, especially in developing countries. In this study a novel assay, combining gold nanoparticle (GNP)-based multiplex oligonucleotide ligation-PCR and universal oligonucleotide microarray technology, was developed for inexpensive, specific, sensitive, and multiplex detection of eight common foodborne pathogens, including Shigella spp., Campylobacter jejuni, Bacillus cereus, Escherichia coli O157:H7, Listeria monocytogenes, Salmonella enterica, Staphylococcus aureus, and Vibrio parahaemolyticus...
May 9, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28476502/quercetin-suppresses-nlrp3-inflammasome-activation-in-epithelial-cells-triggered-by-escherichia-coli-o157-h7
#3
Yansong Xue, Min Du, Mei-Jun Zhu
Inflammatory responses elicited by LRR and PYD domains-containing protein 3 (NLRP3) inflammasome is induced by a wide variety of stress signals including infectious agents and cellular disorders. E. coli O157:H7 causes serious gastrointestinal diseases that results in severe inflammation and oxidative stress, causing host cell damage. In this study, we found that E. coli O157:H7 infection induced NLRP3 assembly, caspase-1 activation and interleukin (IL)-1β and IL-18 release in Caco-2 cells. Infection also resulted in mitochondrial dysfunction with disrupted mitochondrial potential and mitochondrial complex-I activity, as well as the cytosolic release of cytochrome c and altered mitochondrial respiratory chain...
May 2, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28439301/prevalence-virulence-potential-and-pulsed-field-gel-electrophoresis-profiling-of-shiga-toxin-producing-escherichia-coli-strains-from-cattle
#4
Hee-Jin Dong, Soomin Lee, Woohyun Kim, Jae-Uk An, Junhyung Kim, Danil Kim, Seongbeom Cho
BACKGROUND: As a primary source of Shiga-toxin-producing Escherichia coli (STEC) infection, cattle are often targeted to develop strategies for reducing STEC contamination. Monitoring the virulence potentials of STEC isolates from cattle is important for tracing contamination sources, managing outbreaks or sporadic cases, and reducing the risks for human infection. This study aimed to investigate the prevalence of STEC in cattle farm samples in South Korea and to assess their virulence potentials...
2017: Gut Pathogens
https://www.readbyqxmd.com/read/28435260/development-of-gold-nanoparticles-coated-with-silica-containing-the-antibiofilm-drug-cinnamaldehyde-and-their-effects-on-pathogenic-bacteria
#5
Mohankandhasamy Ramasamy, Jin-Hyung Lee, Jintae Lee
Emerging resistance to antibiotics is a mounting worldwide health concern and increases the need for nonantibiotic strategies to combat infectious diseases caused by bacterial pathogens. In this study, the authors used the antibiofilm activity of the naturally occurring antimicrobial cinnamaldehyde (CNMA) conjugated to the surface of gold nanoparticles (GNPs) to deliver CNMA efficiently and eradicate biofilms of Gram-negative organisms (enterohemorrhagic Escherichia coli O157:H7, and Pseudomonas aeruginosa), Gram positive (methicillin-sensitive Staphylococcus aureus organisms, and methicillin-resistant Staphylococcus aureus) bacteria...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28425902/evidence-for-the-benefits-of-food-chain-interventions-on-e-coli-0157-h7-nm-prevalence-in-retail-ground-beef-and-human-disease-incidence-a-success-story
#6
Frank Pollari, Tanya Christidis, Katarina D M Pintar, Andrea Nesbitt, Jeff Farber, Marie-Claude Lavoie, Alex Gill, Penelope Kirsch, Roger P Johnson
OBJECTIVES: Human infection with Escherichia coli O157:H7/NM has historically been associated with consumption of undercooked ground beef. The purpose of this paper is to investigate the correlation of the decline in E. coli O157:H7/NM infections in Canada with the introduction of control efforts in ground beef by industry. METHODS: The human incidence of E. coli O157:H7/NM, prevalence in ground beef and interventions from 1996 to 2014 were analyzed. Pathogen prevalence data were obtained from federal government and industry surveillance and inspection/compliance programs...
April 20, 2017: Canadian Journal of Public Health. Revue Canadienne de Santé Publique
https://www.readbyqxmd.com/read/28421309/genetic-makeup-of-shiga-toxin-producing-escherichia-coli-in-relation-to-clinical-symptoms-and-duration-of-shedding-a-microarray-analysis-of-isolates-from-swedish-children
#7
A Matussek, C Jernberg, I-M Einemo, S Monecke, R Ehricht, I Engelmann, S Löfgren, S Mernelius
Shiga toxin (Stx)-producing Escherichia coli (STECs) cause non-bloody diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome, and are the primary cause of acute renal failure in children worldwide. This study investigated the correlation of genetic makeup of STEC strains as revealed by DNA microarray to clinical symptoms and the duration of STEC shedding. All STEC isolated (n = 96) from patients <10 years of age in Jönköping County, Sweden from 2003 to 2015 were included. Isolates were characterized by DNA microarray, including almost 280 genes...
April 19, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28402463/dynamics-of-insertion-sequence-element-is629-inactivation-of-verotoxin-2-genes-in-escherichia-coli-o157-h7
#8
Heiður Loftsdóttir, Robert Söderlund, Tomas Jinnerot, Erik Eriksson, Erik Bongcam-Rudloff, Anna Aspán
There are several anecdotal reports of insertion sequence (IS)-element inactivation of verotoxin genes among enterohaemorrhagic Escherichia coli of the serotype O157:H7, a pathogen causing severe gastrointestinal disease in infected humans. These insertions can be expected to drastically reduce the virulence of the bacteria. IS-element inactivation has been shown to be reversible in model systems, suggesting the possibility of spontaneous restoration of virulence. In the present study, traditional and high-throughput sequencing was used to characterize three patterns of IS629 inactivation of verotoxin 2 genes in EHEC O157:H7, caused by insertion or insertion followed by partial deletion...
April 11, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28400860/multiple-etiologies-of-infectious-diarrhea-and-concurrent-infections-in-a-pediatric-outpatient-based-screening-study-in-odisha-india
#9
Arpit Kumar Shrivastava, Subrat Kumar, Nirmal Kumar Mohakud, Mrutyunjay Suar, Priyadarshi Soumyaranjan Sahu
BACKGROUND: There are multiple etiologies responsible for infectious gastroenteritis causing acute diarrhea which are often under diagnosed. Also acute diarrhea is one of the major causes of morbidity and mortality among children less than 5 years of age. METHODS: In our study, fecal samples (n = 130) were collected from children (<5 years) presenting with symptoms of acute diarrhea. Samples were screened for viral, bacterial, and parasitic etiologies. Rotavirus and Adenovirus were screened by immunochromatographic tests...
2017: Gut Pathogens
https://www.readbyqxmd.com/read/28397273/internalisation-of-escherichia-coli-o157-h7-gfp-in-rocket-and-swiss-chard-baby-leaves-as-affected-by-abiotic-and-biotic-damage
#10
Rahel Hartmann, Andreas Fricke, Hartmut Stützel, Suzan Mansourian, Teun Dekker, Walter Wohanka, Beatrix Alsanius
Internalisation of human pathogens in edible parts of vegetables eaten raw is a major concern, since once internalised they are protected from sanitising treatments. In this study, we examined invasion of gfp-labelled E. coli O157:H7 into intact and biotically (infection with Xanthomonas campestris/Pseudomonas syringae) and abiotically (grating with silicon carbide) damaged leaves of wild rocket (Diplotaxis tenuifolia) and Swiss chard (Beta vulgaris subsp. cicla) using laser scanning confocal microscopy. Bacterial cells were found in internal locations of the tissue, irrespective of tissue health status...
April 11, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28391537/virulence-factors-of-shiga-toxin-producing-escherichia-coli-and-the-risk-of-developing-haemolytic-uraemic-syndrome-in-norway-1992-2013
#11
U Naseer, I Løbersli, M Hindrum, T Bruvik, L T Brandal
Shiga toxin-producing Escherichia coli (STEC) may cause haemolytic uraemic syndrome (HUS). Age ≤5 years and presence of stx2a and eae are risk factors for the development of HUS. In this study, we investigated STEC isolates for the presence of adhesins, toxins and molecular risk assessment (MRA) factors to identify virulence genes associated with HUS development. We included non-duplicate isolates from all STEC infections (n = 340, HUS = 32) reported to the Norwegian National Reference Laboratory (NRL) for Enteropathogenic Bacteria from 1992 to 2013...
April 8, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28382165/an-electrochemical-strategy-using-multifunctional-nanoconjugates-for-efficient-simultaneous-detection-of-escherichia-coli-o157-h7-and-vibrio-cholerae-o1
#12
Yan Li, Ya Xiong, Lichao Fang, Lili Jiang, Hui Huang, Jun Deng, Wenbin Liang, Junsong Zheng
The rapid and accurate quantification of the pathogenic bacteria is extremely critical to decrease the bacterial infections in all areas related to health and safety. We have developed an electrochemical strategy for simultaneous ultrasensitive detection of E. coli O157:H7 and Vibrio cholerae O1. This approach was based on the specific immune recognition of different pathogenic bacteria by multifunctional nanoconjugates and subsequent signal amplification. By employing the proposed biosensor, the concentrations of these pathogenic bacteria could be established on a single interface in a single run with improved sensitivity and accuracy...
2017: Theranostics
https://www.readbyqxmd.com/read/28368034/protection-of-human-colon-cells-from-shiga-toxin-by-plant-based-recombinant-secretory-iga
#13
Katsuhiro Nakanishi, Shota Morikane, Shiori Ichikawa, Kohta Kurohane, Yasuo Niwa, Yoshihiro Akimoto, Sachie Matsubara, Hayato Kawakami, Hirokazu Kobayashi, Yasuyuki Imai
Shiga toxin is a major virulence factor of food-poisoning caused by Escherichia coli such as O157:H7. Secretory immunoglobulin (Ig) A (SIgA) is supposed to prevent infection of the mucosal surface and is a candidate agent for oral immunotherapy. We previously established a recombinant monoclonal antibody (mAb) consisting of variable regions from a mouse IgG mAb specific for the binding subunit of Shiga toxin 1 (Stx1) and the Fc region of mouse IgA. Here we produced a secretory form of the recombinant IgA (S-hyIgA) with transgenic Arabidopsis thaliana plant...
April 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28360900/oral-immunization-with-recombinant-lactobacillus-acidophilus-expressing-espa-tir-m-confers-protection-against-enterohemorrhagic-escherichia-coli-o157-h7-challenge-in-mice
#14
Ruqin Lin, Yiduo Zhang, Beiguo Long, Yawen Li, Yuhua Wu, Siqin Duan, Bo Zhu, Xianbo Wu, Hongying Fan
Enterohemorrhagic Escherichia coli O157:H7 (EHEC O157:H7) causes hemorrhagic colitis and the formation of characteristic attaching and effacing (A/E) lesions in humans. Given the severe sequelae of EHEC O157:H7 infection, it is critical to develop effective vaccines for human use. However, for achieving this goal many hurdles need to be addressed, such as the type or subset of antigens, adjuvant, and the delivery route. We developed a candidate vaccine by inserting the bivalent antigen espA-Tir-M composed of espA and the Tir central domain into Lactobacillus acidophilus...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28349121/cloacael-carriage-and-multidrug-resistance-escherichia-coli-o157-h7-from-poultry-farms-eastern-ethiopia
#15
Mude Shecho, Naod Thomas, Jelalu Kemal, Yimer Muktar
A cross-sectional study was carried out to determine antimicrobial drug resistance patterns of E. coli O157:H7 isolates and estimate the level of the pathogen. A total of 194 cloacae swab samples were collected randomly in two poultry farms. Standard cultural, biochemical, and serological (latex agglutination) methods were used to isolate E. coli O157:H7. The isolates were subjected to antimicrobial susceptibility testing using disc diffusion method. Out of 194 cloacae samples examined, 13.4% (n = 26) were found to be positive for E...
2017: Journal of veterinary medicine
https://www.readbyqxmd.com/read/28348875/short-term-evolution-of-shiga-toxin-producing-escherichia-coli-o157-h7-between-two-food-borne-outbreaks
#16
Lauren A Cowley, Timothy J Dallman, Stephen Fitzgerald, Neil Irvine, Paul J Rooney, Sean P McAteer, Martin Day, Neil T Perry, James L Bono, Claire Jenkins, David L Gally
Shiga toxin-producing Escherichia coli (STEC) O157:H7 is a public health threat and outbreaks occur worldwide. Here, we investigate genomic differences between related STEC O157:H7 that caused two outbreaks, eight weeks apart, at the same restaurant. Short-read genome sequencing divided the outbreak strains into two sub-clusters separated by only three single-nucleotide polymorphisms in the core genome while traditional typing identified them as separate phage types, PT8 and PT54. Isolates did not cluster with local strains but with those associated with foreign travel to the Middle East/North Africa...
September 2016: Microbial Genomics
https://www.readbyqxmd.com/read/28348764/first-reported-case-of-campylobacter-lanienae-enteritis-in-a-human
#17
Simon Lévesque, Frédéric Lemay, Sadjia Bekal, Eric H Frost, Sophie Michaud
INTRODUCTION: Campylobacters are the most frequently identified bacteria causing diarrhoea in humans worldwide. Campylobacter lanienae was isolated for the first time in 2000 from faecal samples of two asymptomatic abattoir workers in Switzerland during a routine hygiene screen, but has never been associated with human disease. CASE PRESENTATION: At hospital admission, the patient reported diarrhoea, lower abdominal cramps, nausea, one episode of bilious vomiting and low-grade fever of 38 °C...
June 2016: JMM Case Reports
https://www.readbyqxmd.com/read/28343354/unusual-severe-case-of-hemolytic-uremic-syndrome-due-to-shiga-toxin-2d-producing-e-coli-o80-h2
#18
Kioa L Wijnsma, Anne M Schijvens, John W A Rossen, A M D Mirjam Kooistra-Smid, Michiel F Schreuder, Nicole C A J van de Kar
BACKGROUND: Hemolytic uremic syndrome (HUS) is one of the most common causes of acute renal failure in children, with the majority of cases caused by an infection with Shiga toxin-producing Escherichia coli (STEC). Whereas O157 is still the predominant STEC serotype, non-O157 serotypes are increasingly associated with STEC-HUS. However, little is known about this emerging and highly diverse group of non-O157 serotypes. With supportive therapy, STEC-HUS is often self-limiting, with occurrence of chronic sequelae in just a small proportion of patients...
March 25, 2017: Pediatric Nephrology: Journal of the International Pediatric Nephrology Association
https://www.readbyqxmd.com/read/28337193/baicalin-protects-mice-from-lethal-infection-by-enterohemorrhagic-escherichia-coli
#19
Yong Zhang, Zhimin Qi, Yan Liu, Wenqi He, Cheng Yang, Quan Wang, Jing Dong, Xuming Deng
Shiga-like toxin-producing Escherichia coli (STEC) O157:H7 poses grave challenges to public health by its ability to cause severe colonic diseases and renal failure in both human and animals. Shiga-like toxins are the major pathogenic factor for some highly virulent E. coli expecially Shiga-like toxin 2. Conventional treatments such as antibiotics can facilitate the release of the toxin thus potentially exacerbate the diseases. Small molecule inhibitors and antibodies capable of neutralizing the toxins are the two major venues for the development of therapeutics against enterohemorrhagic serotype E...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28321227/co-expression-with-the-type-3-secretion-chaperone-cest-from-enterohemorrhagic-e-coli-increases-accumulation-of-recombinant-tir-in-plant-chloroplasts
#20
Jacqueline MacDonald, Sean Miletic, Typhanie Gaildry, Adam Chin-Fatt, Rima Menassa
Type 3 secretion systems (T3SSs) are utilized by pathogenic Escherichia coli to infect their hosts and many proteins from these systems are affected by chaperones specific to T3SS-containing bacteria. Toward developing a recombinant vaccine against enterohaemorrhagic E. coli (EHEC), we expressed recombinant T3SS and related proteins from predominant EHEC serotypes in Nicotiana chloroplasts. Nicotiana benthamiana were transiently transformed to express chloroplast-targeted Tir, NleA, and EspD from the EHEC serotype O157:H7; a fusion of EspA proteins from serotypes O157:H7 and O26:H11; and a fusion of epitopes of Tir (Tir-ep) from serotypes O157:H7, O26:H11, O45:H2, and O111:H8...
2017: Frontiers in Plant Science
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