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Escherichia Coli O157 infection

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https://www.readbyqxmd.com/read/27920212/inhibition-of-antigen-specific-and-non-specific-stimulation-of-bovine-t-and-b-cells-by-lymphostatin-from-attaching-and-effacing-escherichia-coli
#1
Robin L Cassady-Cain, Elizabeth A Blackburn, Charlotte R Bell, Elizaveta Elshina, Jayne C Hope, Mark P Stevens
Enterohaemorrhagic E. coli (EHEC) and enteropathogenic E. coli (EPEC) are enteric bacterial pathogens of worldwide importance. Most EPEC and non-O157 EHEC strains express lymphostatin (also known as LifA), a chromosomally-encoded 365 kDa protein. We previously demonstrated that lymphostatin is a putative glycosyltransferase that is important in intestinal colonisation of cattle by EHEC serogroup O5, O111 and O26 strains. However, the nature and consequences of the interaction between lymphostatin and immune cells from the bovine host are ill-defined...
December 5, 2016: Infection and Immunity
https://www.readbyqxmd.com/read/27908998/whole-genome-sequencing-applied-to-the-molecular-epidemiology-of-shiga-toxin-producing-escherichia-coli-o157-h7-in-argentina
#2
Claudia Carolina Carbonari, Nahuel Fittipaldi, Sarah Teatero, Taryn B T Athey, Luis Pianciola, Marcelo Masana, Roberto G Melano, Marta Rivas, Isabel Chinen
Shiga toxin-producing Escherichia coli strains are worldwide associated with sporadic human infections and outbreaks. In this work, we report the availability of high-quality draft whole-genome sequences for 19 O157:H7 strains isolated in Argentina.
December 1, 2016: Genome Announcements
https://www.readbyqxmd.com/read/27854514/subtyping-escherichia-coli-virulence-genes-isolated-from-feces-of-beef-cattle-and-clinical-cases-in-alberta
#3
Renata Tostes, Noriko Goji, Kingsley Amoako, Linda Chui, John Kastelic, Rebekah DeVinney, Kim Stanford, Tim Reuter
Clinical outcomes of Shiga toxin (stx)-producing Escherichia coli infection are largely determined by virulence gene subtypes. This study used a polymerase chain reaction (PCR)-pyrosequencing assay to analyze single-nucleotide polymorphisms for subtyping three major virulence genes (stx1, stx2, eae) of pathogenic E. coli (O157, O26, O111, and O103) isolated from cattle over a 2-year interval (n = 465) and human clinical cases (n = 42) in western Canada. Most bovine isolates were PCR positive for at least one target virulence gene (367/465), whereas 100% of human isolates harbored eae in combination with at least one stx gene...
November 17, 2016: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/27853704/molecular-and-phylogenetic-characterization-of-non-o157-shiga-toxin-producing-escherichia-coli-strains-in-china
#4
Xiangning Bai, Bin Hu, Yanmei Xu, Hui Sun, Ailan Zhao, Pengbin Ba, Shanshan Fu, Ruyue Fan, Yujuan Jin, Hong Wang, Qiusheng Guo, Xuebin Xu, Shan Lu, Yanwen Xiong
Shiga toxin-producing Escherichia coli (STEC) causes diarrhea and hemorrhagic colitis with life-threatening complications, such as hemolytic uremic syndrome. The aim of this study was to assess the molecular epidemiologic features of non-O157 STEC strains from different resources in China and illustrate the role of animal reservoirs or animal-derived foodstuffs in human STEC infections. A collection of 301 non-O157 STEC isolates from domestic and wild animals (i.e., cattle, goat, pig, yak, pika, and antelope), raw meats (i...
2016: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/27770538/rapid-identification-of-the-etiological-factors-causing-diarrheal-diseases
#5
G Kamkamidze, I Migriauli, D Razmadze, M Kochlamazashvili, K Mulkijanyan, M Butsashvili
The main objective of this investigation was to develop and pilot a real-time Polymerase Chain Reaction (rt-PCR) diagnostic system for rapid and simultaneous identification of pathogens with a particular emphasis on diarrheal disease diagnostics. The diarrheal diseases were selected as a target for the pilot because they constitute a primary public health priority in Georgia and worldwide. The product developed by our research team "Neo_PCR_Diagnostics" represents an original system for the identification of pathogens associated with gastrointestinal tract infections and diarrhea...
September 2016: Georgian Medical News
https://www.readbyqxmd.com/read/27713209/virulence-factors-and-stability-of-coliphages-specific-to-escherichia-coli-o157-h7-and-to-various-e-coli-infection
#6
Eun-Jin Kim, Hyun-Joo Chang, Soojin Kwak, Jong-Hyun Park
Characteristics of E.coli O157:H7 specific infection bacteriophages (O157 coliphages) and broad-host-range bacteriophages for other E.coli serotypes (broad-host coliphages) were compared. The burst sizes of two groups were ranged from 40 to 176 PFU/infected. Distribution of virulence factors of stx1, stx2, ehxA, and saa between two groups were not differenciated. Broad host coliphages showed a little stable at 70°C than O157 coliphages. However, O157 coliphages showed high acid and ethanol tolerant by reduction of only 22% and 11% phages under pH 3 and 70% ethanol for an hour exposure...
October 6, 2016: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27678285/antimicrobial-resistance-in-shiga-toxin-producing-escherichia-coli-serogroups-o157-and-o26-isolated-from-human-cases-of-diarrhoeal-disease-in-england-2015
#7
Martin Day, Michel Doumith, Claire Jenkins, Timothy J Dallman, Katie L Hopkins, Richard Elson, Gauri Godbole, Neil Woodford
OBJECTIVES: Shiga toxin-producing Escherichia coli (STEC) are zoonotic and transmission to humans occurs via contaminated food or contact with infected animals. In this study, WGS data were used to predict antimicrobial resistance (AMR) in STEC from symptomatic human cases to assess the extent of transmission of antibiotic-resistant E. coli from animals to humans. METHODS: WGS data from 430 isolates of STEC were mapped to genes known to be associated with phenotypic AMR...
September 27, 2016: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/27672499/characterization-of-novel-bacteriophage-phic119-capable-of-lysing-multidrug-resistant-shiga-toxin-producing-escherichia-coli-o157-h7
#8
Luis Amarillas, Cristóbal Chaidez, Arturo González-Robles, Yadira Lugo-Melchor, Josefina León-Félix
BACKGROUND: Shiga toxin-producing Escherichia coli (STEC) is one of the most common and widely distributed foodborne pathogens that has been frequently implicated in gastrointestinal and urinary tract infections. Moreover, high rates of multiple antibiotic-resistant E. coli strains have been reported worldwide. Due to the emergence of antibiotic-resistant strains, bacteriophages are considered an attractive alternative to biocontrol pathogenic bacteria. Characterization is a preliminary step towards designing a phage for biocontrol...
2016: PeerJ
https://www.readbyqxmd.com/read/27672437/preparation-and-characterization-of-photoactive-antimicrobial-graphitic-carbon-nitride-g-c3n4-films
#9
John H Thurston, Necia M Hunter, Kenneth A Cornell
Photoactive films derived from nanostructured samples of metal-free, intermediate band gap semiconductor graphitic carbon nitride (ns-g-C3N4) have been synthesized and characterized for their particle properties and antimicrobial activity. Physical characterization reveals that these materials are composed of discrete nanoparticles whose dimensions range from 200 nm to 700 nm. Investigation of the photochemical reactivity of ns-g-C3N4 using coumarin-3-carboxylic acid (3-CCA) indicates that this material produces reactive oxygen species (ROS) under visible radiation...
2016: RSC Advances
https://www.readbyqxmd.com/read/27650979/anti-bacterial-effect-of-essential-oil-from-xanthium-strumarium-against-shiga-toxin-producing-escherichia-coli
#10
J Sharifi-Rad, L Soufi, S A M Ayatollahi, M Iriti, M Sharifi-Rad, E M Varoni, F Shahri, S Esposito, K Kuhestani, M Sharifi-Rad
Shiga toxin-producing Escherichia coli (STEC) serotype O157:H7 is one of the most important human pathogenic microorganisms, which can cause life-threatening infections. Xanthium strumarium L. is a plant with anti-bacterial activity against gram-negative and gram-positive bacteria. This study aims to demonstrate in vitro efficacy of the essential oil (EO) extracted from Xanthium strumarium L. against E. coli O157:H7. Using the agar test diffusion, the effect of Xanthium strumarium L. EO (5, 10, 15, 30, 60, and 120 mg/mL) was verified at each of the four different growth phases of E...
2016: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/27625996/from-in-silico-protein-epitope-density-prediction-to-testing-escherichia-coli-o157-h7-vaccine-candidates-in-a-murine-model-of-colonization
#11
Daniel Tapia, Brittany N Ross, Anjana Kalita, Mridul Kalita, Christopher L Hatcher, Laura A Muruato, Alfredo G Torres
Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a leading cause of foodborne illnesses worldwide and is a common serotype linked to hemorrhagic colitis and an important cause of hemolytic uremic syndrome (HUS). Treatment of EHEC O157:H7 infections is complicated, as antibiotics can exacerbate Shiga toxin (Stx) production and lead to more severe symptoms including HUS. To date, no vaccines have been approved for human use, exposing a void in both treatment and prevention of EHEC O157:H7 infections. Previously, our lab has shown success in identifying novel vaccine candidates via bio- and immunoinformatics approaches, which are capable of reducing bacterial colonization in an in vivo model of intestinal colonization...
2016: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/27624517/the-use-of-a-novel-nanoluc-based-reporter-phage-for-the-detection-of-escherichia-coli-o157-h7
#12
Dandan Zhang, Claudia P Coronel-Aguilera, Patricia L Romero, Lynda Perry, Udit Minocha, Carla Rosenfield, Andrew G Gehring, George C Paoli, Arun K Bhunia, Bruce Applegate
Rapid detection of the foodborne pathogen Escherichia coli O157:H7 is of vital importance for public health worldwide. Among detection methods, reporter phages represent unique and sensitive tools for the detection of E. coli O157:H7 from food as they are host-specific and able to differentiate live cells from dead ones. Upon infection, target bacteria become identifiable since reporter genes are expressed from the engineered phage genome. The E. coli O157:H7 bacteriophage ΦV10 was modified to express NanoLuc luciferase (Nluc) derived from the deep-sea shrimp Oplophorus gracilirostris...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27622632/identification-of-enteropathogenic-escherichia-coli-in-children-with-acute-diarrheic-syndrome-from-sucre-state-venezuela
#13
Elvia Michelli, Adriana Millán, Hectorina Rodulfo, Mirian Michelli, Jesús Luiggi, Numirin Carreño, Marcos De Donato
INTRODUCTION: Diarrheagenic Escherichia coli is an important causative agent of acute diarrheic syndrome.  OBJECTIVE: To identify clonal groups of enteropathogenic E. coli (EPEC), in 485 children with acute diarrhea aged 0 to 10 years attending health care centers in Arismendi, Benítez and Sucre municipalities, Sucre state, Venezuela, from March to December, 2011.  MATERIALS AND METHODS: After obtaining the informed consent, stool samples were collected...
2016: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/27618439/comprehensive-evaluation-and-implementation-of-improvement-actions-in-butcher-shops
#14
Gerardo A Leotta, Victoria Brusa, Lucía Galli, Cristian Adriani, Luciano Linares, Analía Etcheverría, Marcelo Sanz, Adriana Sucari, Pilar Peral García, Marcelo Signorini
Foodborne pathogens can cause acute and chronic diseases and produce a wide range of symptoms. Since the consumption of ground beef is a risk factor for infections with some bacterial pathogens, we performed a comprehensive evaluation of butcher shops, implemented improvement actions for both butcher shops and consumers, and verified the impact of those actions implemented. A comprehensive evaluation was made and risk was quantified on a 1-100 scale as high-risk (1-40), moderate-risk (41-70) or low-risk (71-100)...
2016: PloS One
https://www.readbyqxmd.com/read/27609320/a-geostatistical-investigation-of-agricultural-and-infrastructural-risk-factors-associated-with-primary-verotoxigenic-e-coli-vtec-infection-in-the-republic-of-ireland-2008-2013
#15
C Óhaiseadha, P D Hynds, U B Fallon, J O'Dwyer
Ireland reports the highest incidence of verotoxigenic Escherichia coli (VTEC) infection in Europe. This study investigated potential risk factors for confirmed sporadic and outbreak primary VTEC infections during 2008-2013. Overall, 989 VTEC infections including 521 serogroup O157 and 233 serogroup O26 were geo-referenced to 931 of 18 488 census enumeration areas. The geographical distribution of human population, livestock, unregulated groundwater sources, domestic wastewater treatment systems (DWWTS) and a deprivation index were examined relative to notification of VTEC events in 524 of 6242 rural areas...
January 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/27607835/presence-of-escherichia-coli-o157-salmonella-spp-and-listeria-monocytogenes-in-raw-ovine-milk-destined-for-cheese-production-and-evaluation-of-the-equivalence-between-the-analytical-methods-applied
#16
Giulia Amagliani, Annalisa Petruzzelli, Elisa Carloni, Franco Tonucci, Martina Foglini, Eleonora Micci, Mariagrazia Ricci, Stefania Di Lullo, Luca Rotundo, Giorgio Brandi
Italy is one of the main producers and exporters of cheese made from unpasteurized sheep milk. Since raw milk and its derived products are known sources of human infections, cheese produced from raw sheep milk could pose a microbiological threat to public health. Hence, the objectives of the study were: to characterize the potential risk of the presence of pathogens Escherichia coli O157, Listeria monocytogenes, and Salmonella in raw ovine milk destined for cheese production obtained from all the sheep farms (n = 24) in the Marches region (Central Italy) that directly transform raw milk into cheeses and to evaluate the equivalence between the analytical methods applied...
September 8, 2016: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/27603508/development-of-a-sandwich-elisa-for-ehec-o157-h7-intimin-%C3%AE-1
#17
Xuehan Zhang, Meng Li, Bicheng Zhang, Kangming Chen, Kongwang He
Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a zoonotic pathogen of worldwide importance that causes foodborne infections in humans. Intimin gamma 1 (intimin γ1) is one of the most important outer membrane proteins required for EHEC's intimate adhesion to epithelial cells. Here, we generated a polyclonal antibody (pAb) and a monoclonal antibody (mAb) against intimin γ1 to develop a double antibody sandwich ELISA (DAS-ELISA) with increased sensitivity and specificity for measuring EHEC O157:H7. To achieve this goal, a rabbit pAb was used as a capture antibody, and a mouse mAb was a detection antibody...
2016: PloS One
https://www.readbyqxmd.com/read/27600997/experimental-infection-of-calves-with-escherichia-coli-o104-h4-outbreak-strain
#18
K Hamm, S A Barth, S Stalb, L Geue, E Liebler-Tenorio, J P Teifke, E Lange, K Tauscher, G Kotterba, M Bielaszewska, H Karch, C Menge
In 2011, a severe outbreak of hemolytic-uremic syndrome was caused by an unusual, highly virulent enterohemorrhagic E. coli (EHEC) O104:H4 strain, which possessed EHEC virulence traits in the genetic background of human-adapted enteroaggregative E. coli. To determine magnitude of fecal shedding and site of colonization of EHEC O104:H4 in a livestock host, 30 (ten/strain) weaned calves were inoculated with 10(10) CFU of EHEC O104:H4, EHEC O157:H7 (positive control) or E. coli strain 123 (negative control) and necropsied (4 or 28 d...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27586477/computational-determination-of-the-effects-of-virulent-escherichia-coli-and-salmonella-bacteriophages-on-human-gut
#19
Marwa Mostafa Mostafa, Mohammad Nassef, Amr Badr
BACKGROUND AND OBJECTIVE: Salmonella and Escherichia coli are different types of bacteria that cause food poisoning in humans. In the elderly, infants and people with chronic conditions, it is very dangerous if Salmonella or E. coli gets into the bloodstream and then they must be treated by phage therapy. Treating Salmonella and E. coli by phage therapy affects the gut flora. This research paper presents a system for detecting the effects of virulent E. coli and Salmonella bacteriophages on human gut...
October 2016: Computer Methods and Programs in Biomedicine
https://www.readbyqxmd.com/read/27578754/new-insights-into-the-formation-of-viable-but-nonculturable-escherichia-coli-o157-h7-induced-by-high-pressure-co2
#20
Feng Zhao, Yongtao Wang, Haoran An, Yanling Hao, Xiaosong Hu, Xiaojun Liao
UNLABELLED: The formation of viable but nonculturable (VBNC) Escherichia coli O157:H7 induced by high-pressure CO2 (HPCD) was investigated using RNA sequencing (RNA-Seq) transcriptomics and isobaric tag for relative and absolute quantitation (iTRAQ) proteomic methods. The analyses revealed that 97 genes and 56 proteins were significantly changed upon VBNC state entry. Genes and proteins related to membrane transport, central metabolisms, DNA replication, and cell division were mainly downregulated in the VBNC cells...
2016: MBio
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