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Escherichia Coli O157 infection

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https://www.readbyqxmd.com/read/29342189/the-microbiota-metabolite-indole-inhibits-salmonella-virulence-involvement-of-the-phopq-two-component-system
#1
Nandita Kohli, Zeni Crisp, Rebekah Riordan, Michael Li, Robert C Alaniz, Arul Jayaraman
The microbial community present in the gastrointestinal tract is an important component of the host defense against pathogen infections. We previously demonstrated that indole, a microbial metabolite of tryptophan, reduces enterohemorrhagic Escherichia coli O157:H7 attachment to intestinal epithelial cells and biofilm formation, suggesting that indole may be an effector/attenuator of colonization for a number of enteric pathogens. Here, we report that indole attenuates Salmonella Typhimurium (Salmonella) virulence and invasion as well as increases resistance to colonization in host cells...
2018: PloS One
https://www.readbyqxmd.com/read/29326391/zoonotic-bacteria-and-parasites-found-in-raw-meat-based-diets-for-cats-and-dogs
#2
Freek P J van Bree, Gertie C A M Bokken, Robin Mineur, Frits Franssen, Marieke Opsteegh, Joke W B van der Giessen, Len J A Lipman, Paul A M Overgaauw
Feeding raw meat-based diets (RMBDs) to companion animals has become increasingly popular. Since these diets may be contaminated with bacteria and parasites, they may pose a risk to both animal and human health. The purpose of this study was to test for the presence of zoonotic bacterial and parasitic pathogens in Dutch commercial RMBDs. We analysed 35 commercial frozen RMBDs from eight different brands. Escherichia coli serotype O157:H7 was isolated from eight products (23 per cent) and extended-spectrum beta-lactamases-producing E coli was found in 28 products (80 per cent)...
January 13, 2018: Veterinary Record
https://www.readbyqxmd.com/read/29279448/comparison-of-three-molecular-subtyping-methods-among-o157-and-non-o157-shiga-toxin-producing-escherichia-coli-isolates-from-japanese-cattle
#3
Hiromi Nakamura, Atsushi Iguchi, Tomofumi Maehara, Kyohei Fujiwara, Atsushi Fujiwara, Jun Ogasawara
To determine the infection source, route, and extent of outbreak, it is important to subtype Shiga toxin-producing Escherichia coli (STEC) isolates belonging to the same serotype for clustering into clonally related groups. In this study, we compared three molecular subtyping methods, multi-locus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and multiple-locus variable number of tandem repeat analysis (MLVA), using O157 and non-O157 STEC isolated from Japanese beef cattle. A total of 73 STEC isolates belonging to 9 O-serogroups were used...
December 26, 2017: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29273508/prevalence-quantification-and-isolation-of-pathogenic-shiga-toxin-escherichia-coli-o157-h7-along-the-production-and-supply-chain-of-pork-around-hubei-province-of-china
#4
Sher Bahadar Khan, Geng Zou, Ran Xiao, Yuting Cheng, Zia Ur Rehman, Sher Ali, Atta Muhammad Memon, Shah Fahad, Irshad Ahmad, Rui Zhou
Shiga toxin Escherichia coli (STEC) O157:H7 is an important zoonotic food borne pathogen causing gastroenteritis that may lead to life threatening hemorragic colitis (HC) and hemorrhagic uremic syndrome (HUS). 325 meat and tissue samples were tested for enumeration of O157:H7 strains using most probable number (MPN)-PCR targeting their specific genes flicH7 and rfbO157 followed by isolation, sereotyping and pathogenicity testing. The overall prevalence of O157:H7 was 41.3% (134/325) along the production and supply chain of pork (PSCP), being higher in supply chain (59%, 118/200) as compared to pig farms (12...
December 19, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29260688/geogenomic-segregation-and-temporal-trends-of-human-pathogenic-escherichia-coli-o157-h7-washington-usa-2005-20141
#5
Gillian A M Tarr, Smriti Shringi, Amanda I Phipps, Thomas E Besser, Jonathan Mayer, Hanna N Oltean, Jon Wakefield, Phillip I Tarr, Peter Rabinowitz
The often-noted and persistent increased incidence of Escherichia coli O157:H7 infections in rural areas is not well understood. We used a cohort of E. coli O157:H7 cases reported in Washington, USA, during 2005-2014, along with phylogenomic characterization of the infecting isolates, to identify geographic segregation of and temporal trends in specific phylogenetic lineages of E. coli O157:H7. Kernel estimation and generalized additive models demonstrated that pathogen lineages were spatially segregated during the period of analysis and identified a focus of segregation spanning multiple, predominantly rural, counties for each of the main clinical lineages, Ib, IIa, and IIb...
January 2018: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/29227212/screening-for-host-proteins-interacting-with-escherichia-coli-o157-h7-espf-using-bimolecular-fluorescence-complementation
#6
Ying Hua, Jingwei Ju, Xiangyu Wang, Bao Zhang, Wei Zhao, Qiwei Zhang, Yingzhu Feng, Wenbin Ma, Chengsong Wan
AIM: To screen host proteins that interact with enterohemorrhagic Escherichia coli O157:H7 EspF. MATERIALS & METHODS: Flow cytometry and high-throughput sequencing were used to screen interacting proteins. Molecular function, biological processes and Kyoto Encyclopedia of Genes and Genomes pathways were studied using the DAVID online tool. Glutathione S-transferase pull down and dot blotting were used to verify the interactions. RESULTS: 293 host proteins were identified to associate with EspF...
December 11, 2017: Future Microbiology
https://www.readbyqxmd.com/read/29216383/genetic-susceptibility-to-hemolytic-uremic-syndrome-after-shiga-toxin-producing-escherichia-coli-stec-infection-a-centers-for-disease-control-and-prevention-cdc-foodnet-study
#7
Asha R Kallianpur, Yuki Bradford, Rajal K Mody, Katie N Garman, Nicole Comstock, Sarah L Lathrop, Carol Lyons, Amy Saupe, Katie Wymore, Jeffrey A Canter, Lana M Olson, Amanda Palmer, Timothy F Jones
Background: Post-diarrheal hemolytic-uremic syndrome (D+HUS) following Shiga toxin-producing Escherichia coli (STEC) infection is a serious condition lacking specific treatment. Host immune dysregulation and genetic susceptibility to complement hyperactivation are implicated in non-STEC-related HUS. However, genetic susceptibility to D+HUS remains largely uncharacterized. Methods: Patients with culture-confirmed STEC diarrhea, identified through the CDC FoodNet surveillance system (2007-2012), were serotyped and classified by laboratory and/or clinical criteria as suspected, probable, or confirmed D+HUS, or as controls and genotyped at 200 loci linked to non-diarrheal HUS or similar pathologies...
December 6, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29214000/role-of-antigen-43-on-biofilm-formation-and-horizontal-antibiotic-resistance-gene-transfer-in-non-o157-shiga-toxin-producing-escherichia-coli-strains
#8
Roholla Taghadosi, Mohammad Reza Shakibaie, Reza Ghanbarpour, Hossein Hosseini-Nave
Background and Objectives: The objectives of this study were to evaluate the antibiotic resistance profiles, biofilm formation, presence of antigen 43 (Ag43) gene, and transfer of antibiotic resistance phenotype among non-O157 Shiga toxin producing Escherichia coli (STEC). Materials and Methods: From October 2014 to November 2015 a total of 276 stool samples were collected from healthy calves, goats and 395 patients with the sign of nonbloody diarrhea and screened for presence of stx and serotype O157 genes by polymerase chain reaction (PCR) technique...
April 2017: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29208163/population-structure-of-escherichia-coli-o26%C3%A2-%C3%A2-h11-with-recent-and-repeated-stx2-acquisition-in-multiple-lineages
#9
Yoshitoshi Ogura, Yasuhiro Gotoh, Takehiko Itoh, Mitsuhiko P Sato, Kazuko Seto, Shyuji Yoshino, Junko Isobe, Yoshiki Etoh, Mariko Kurogi, Keiko Kimata, Eriko Maeda, Denis Piérard, Masahiro Kusumoto, Masato Akiba, Kiyoshi Tominaga, Yumi Kirino, Yuki Kato, Katsuhiko Shirahige, Tadasuke Ooka, Nozomi Ishijima, Ken-Ichi Lee, Sunao Iyoda, Jacques Georges Mainil, Tetsuya Hayashi
A key virulence factor of enterohaemorrhagic Escherichia coli (EHEC) is the bacteriophage-encoded Shiga toxin (Stx). Stxs are classified into two types, Stx1 and Stx2, and Stx2-producing strains are thought to cause more severe infections than strains producing only Stx1. Although O26 : H11 is the second most prevalent EHEC following O157 : H7, the majority of O26 : H11 strains produce Stx1 alone. However, Stx2-producing O26 strains have increasingly been detected worldwide. Through a large-scale genome analysis, we present a global phylogenetic overview and evolutionary timescale for E...
November 2017: Microbial Genomics
https://www.readbyqxmd.com/read/29177093/patchy-promiscuity-machine-learning-applied-to-predict-the-host-specificity-of-salmonella-enterica-and-escherichia-coli
#10
Nadejda Lupolova, Tim J Dallman, Nicola J Holden, David L Gally
Salmonella enterica and Escherichia coli are bacterial species that colonize different animal hosts with sub-types that can cause life-threatening infections in humans. Source attribution of zoonoses is an important goal for infection control as is identification of isolates in reservoir hosts that represent a threat to human health. In this study, host specificity and zoonotic potential were predicted using machine learning in which Support Vector Machine (SVM) classifiers were built based on predicted proteins from whole genome sequences...
October 2017: Microbial Genomics
https://www.readbyqxmd.com/read/29173628/survival-of-escherichia-coli-o157-h7-during-the-manufacture-and-ripening-of-cacioricotta-goat-cheese
#11
F Ioanna, N C Quaglia, M M Storelli, D Castiglia, E Goffredo, A Storelli, M De Rosa, G Normanno, A Caputi Jambrenghi, A Dambrosio
The aim of this study was to assess the growth and survival of Escherichia coli O157:H7 during the manufacturing and ripening of Cacioricotta goat cheese. Goat milk was artificially contaminated with E. coli O157:H7 and the bacterial load was monitored from production up to 90 days of ripening. Goat milk was inoculated with 102 cfu ml-1 of E. coli O157:H7 and the bacterial count of the curd at time zero was 2.31 log10 cfu g-1. During the first day of ripening, the bacterial load has increased to 5.73 log10 cfu g-1 to more than 6...
April 2018: Food Microbiology
https://www.readbyqxmd.com/read/29157822/antibiofilm-activities-of-norharmane-and-its-derivatives-against-escherichia-coli-o157-h7-and-other-bacteria
#12
Jin-Hyung Lee, Yong-Guy Kim, Sang Hee Shim, Jintae Lee
BACKGROUND: Bacterial biofilms exhibit reduced sensitivity to conventional antibiotics and host defence systems and contribute to the persistence of chronic bacterial infections. HYPOTHESIS: The antibiofilm approach using plant alkaloids provides an alternative to antibiotic strategies. STUDY DESIGN: In this study, the antibiofilm activities of various plant alkaloids were investigated against enterohemorrhagic Escherichia coli O157:H7 and Pseudomonas aeruginosa...
December 1, 2017: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/29157370/bayesian-estimation-of-true-prevalence-sensitivity-and-specificity-of-three-diagnostic-tests-for-detection-of-escherichia-coli-o157-in-cattle-feces
#13
Pius S Ekong, Michael W Sanderson, Nora M Bello, Lance W Noll, Natalia Cernicchiaro, David G Renter, Jianfa Bai, T G Nagaraja
Cattle are a reservoir for Escherichia coli O157 and they shed the pathogen in their feces. Fecal contaminants on the hides can be transferred onto carcasses during processing at slaughter plants, thereby serving as a source of foodborne infection in humans. The detection of E. coli O157 in cattle feces is based on culture, immunological, and molecular methods We evaluated the diagnostic sensitivity and specificity of one culture- and two PCR-based tests for the detection of E. coli O157 in cattle feces, and its true prevalence using a Bayesian implementation of latent class models...
December 1, 2017: Preventive Veterinary Medicine
https://www.readbyqxmd.com/read/29132042/long-range-interactions-keep-bacterial-cells-from-liquid-solid-interfaces-evidence-of-a-bacteria-exclusion-zone-near-nafion-surfaces-and-possible-implications-for-bacterial-attachment
#14
Yifan Cheng, Carmen I Moraru
Hydrophilic surfaces of both abiotic and biological origin have been shown to bear particle-exclusion zones as large as hundreds of micrometers at liquid-solid interfaces. Here we present the first systematic investigation and evidence for bacteria-free exclusion zones for several bacterial strains, including pathogens associated with hospital infections and/or foodborne outbreaks: Staphylococcus aureus, Escherichia coli O157:H7, and Listeria monocytogenes. Tests were carried out both in a phosphate buffer, as well as triptic soy broth (TSB) of high ionic strength...
November 10, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29123549/isolation-and-physiomorphological-characterization-of-escherichia-coli-o157-h7-infecting-bacteriophages-recovered-from-beef-cattle-operations
#15
Pushpinder Kaur Litt, Divya Jaroni
Bacteriophages, recovered from beef cattle environment and specifically targeting Escherichia coli O157:H7, were examined for their physiological and morphological characteristics. Degree of bacterial lysis and host range of isolated bacteriophages was determined against 55 isolates of E. coli O157:H7. Morphology of phages was examined under transmission electron microscope. Phage growth parameters, particularly rate of adsorption, rise period, latent period, and burst size were also determined. The stability of isolated phages was tested at acidic and alkaline pH, at high temperatures, and in cold storage...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/29115532/application-of-f0f1%C3%A2-atpase-immuno%C3%A2-biosensors-for-detecting-escherichia%C3%A2-coli-o157-h7
#16
Ling Wei, Bao-Ming Li, Cheng-Bin Wang, Zi-Jia Kang, Jie Sun, Hui-Juan Wu, Yong-Zhi Lun
Escherichia coli (E. coli) O157:H7 is an important food‑borne pathogen with a low infective threshold and high resistance to treatment. There are currently a number of detection methods available, however, the majority are time‑consuming, complex and expensive, thus it is hard for these methods to be applied in routine detection. Therefore, there is urgent requirement to develop more sensitive, rapid and specific detective techniques. In the present study, an immuno‑biosensor based on the interference of load to the F0F1‑ATPase rotation, indicated by the fluorescence fluctuation, was constructed to detect O157:H7...
November 7, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29111613/management-of-shiga-toxin-producing-escherichia-coli-infected-children-a-multi-national-multi-specialty-survey
#17
Stephen B Freedman, Silviu Grisaru, Jianling Xie, Susan Samuel, Andrew Dixon, Amy C Plint, David Schnadower
AIM: Research has highlighted the potential role that hydration status may play in predicting outcomes in Shiga toxin-producing Escherichia coli (STEC)-infected children. Because little is known about the management of STEC-infected children in the pre-haemolytic uremic syndrome phase, we compared paediatric emergency medicine and nephrologist-stated management approaches to STEC-infected children. METHODS: Members of the Pediatric Emergency Research Canada (PERC; n = 228), the Pediatric Emergency Medicine Collaborative Research Committee (PEM CRC; n = 221) and the Canadian Association of Pediatric Nephrologists (CAPN; n = 66) were surveyed...
November 7, 2017: Journal of Paediatrics and Child Health
https://www.readbyqxmd.com/read/29091926/lactobacillus-reuteri-suppresses-e-coli-o157-h7-in-bovine-ruminal-fluid-toward-a-pre-slaughter-strategy-to-improve-food-safety
#18
Yolande Bertin, Chloé Habouzit, Lysiane Dunière, Marie Laurier, Alexandra Durand, David Duchez, Audrey Segura, Delphine Thévenot-Sergentet, Federico Baruzzi, Frédérique Chaucheyras-Durand, Evelyne Forano
The bovine gastrointestinal tract (GIT) is the main reservoir for enterohaemorrhagic Escherichia coli (EHEC) responsible for food-borne infections. Therefore, it is crucial to develop strategies, such as EHEC suppression by antagonistic microorganisms, to reduce EHEC survival in the GIT of cattle and to limit shedding and food contamination. Most human-derived Lactobacillus reuteri strains produce hydroxypropionaldehyde (HPA), an antimicrobial compound, during anaerobic reduction of glycerol. The capacity of L...
2017: PloS One
https://www.readbyqxmd.com/read/29080954/microbiological-risk-infection-assessment-using-qmra-in-agriculture-systems-in-c%C3%A3-te-d-ivoire-west-africa
#19
Parfait K Kouamé, Hung Nguyen-Viet, Kouassi Dongo, Christian Zurbrügg, Jean Biémi, Bassirou Bonfoh
Poor wastewater management that results from a lack of appropriate sanitation infrastructure contributes to increasing health risks in urban areas in Côte d'Ivoire. We assessed the health risks associated with the use of wastewater for watering salad destined for human consumption, to help local authorities in developing appropriate risk mitigation measures for Yamoussoukro, the political capital of Côte d'Ivoire. We applied a stochastic approach based on quantitative microbiological risk assessment (QMRA), focusing on wastewater for farming activities and salad consumption at the household level...
October 28, 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/29080423/prevalence-and-characteristics-of-shiga-toxin-producing-escherichia-coli-in-finishing-pigs-implications-on-public-health
#20
Wonhee Cha, Pina M Fratamico, Leah E Ruth, Andrew S Bowman, Jacqueline M Nolting, Shannon D Manning, Julie A Funk
Shiga toxin-producing Escherichia coli (STEC) are important food-borne pathogens, which can cause serious illnesses, including hemorrhagic colitis and hemolytic uremic syndrome. To study the epidemiology of STEC in finishing pigs and examine the potential risks they pose for human STEC infections, we conducted a longitudinal cohort study in three finishing sites. Six cohorts of pigs (2 cohorts/site, 20 pigs/cohort) were randomly selected, and fecal samples (n=898) were collected every two weeks through their finishing period...
October 21, 2017: International Journal of Food Microbiology
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