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Escherichia Coli O157 H7 treatment

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https://www.readbyqxmd.com/read/29130764/efficacy-of-a-blend-of-sulfuric-acid-and-sodium-sulfate-against-shiga-toxin-producing-escherichia-coli-salmonella-and-nonpathogenic-escherichia-coli-biotype-i-on-inoculated-prerigor-beef-surface-tissue
#1
Britteny R Scott-Bullard, Ifigenia Geornaras, Robert J Delmore, Dale R Woerner, James O Reagan, J Bred Morgan, Keith E Belk
A study was conducted to investigate the efficacy of a sulfuric acid-sodium sulfate blend (SSS) against Escherichia coli O157:H7, non-O157 Shiga toxin-producing E. coli (STEC), Salmonella, and nonpathogenic E. coli biotype I on prerigor beef surface tissue. The suitability of using the nonpathogenic E. coli as a surrogate for in-plant validation studies was also determined by comparing the data obtained for the nonpathogenic inoculum with those for the pathogenic inocula. Prerigor beef tissue samples (10 by 10 cm) were inoculated (ca...
December 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/29115532/application-of-f0f1%C3%A2-atpase-immuno%C3%A2-biosensors-for-detecting-escherichia%C3%A2-coli-o157-h7
#2
Ling Wei, Bao-Ming Li, Cheng-Bin Wang, Zi-Jia Kang, Jie Sun, Hui-Juan Wu, Yong-Zhi Lun
Escherichia coli (E. coli) O157:H7 is an important food‑borne pathogen with a low infective threshold and high resistance to treatment. There are currently a number of detection methods available, however, the majority are time‑consuming, complex and expensive, thus it is hard for these methods to be applied in routine detection. Therefore, there is urgent requirement to develop more sensitive, rapid and specific detective techniques. In the present study, an immuno‑biosensor based on the interference of load to the F0F1‑ATPase rotation, indicated by the fluorescence fluctuation, was constructed to detect O157:H7...
November 7, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29111062/plasma-treated-poly-ethylene-oxide-nanofibers-containing-tea-tree-oil-beta-cyclodextrin-inclusion-complex-for-antibacterial-packaging
#3
Haiying Cui, Mei Bai, Lin Lin
This work describes the effect of cold nitrogen plasma to enhance the antibacterial activity of poly(ethylene oxide) (PEO) nanofibers containing antibacterial agent. Beta-cyclodextrin (β-CD) and tea tree oil (TTO) were used as a host-guest to form water-soluble inclusion complex. The encapsulation efficiency of TTO in inclusion complex could reach 73.23% at 60°C. As antibacterial agent, the inclusion complex was encapsulated into PEO matrix by electrospun. After plasma treatment, the release efficiency of antibacterial agent from PEO nanofibers was improved...
January 1, 2018: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29072326/antimicrobial-activity-of-suspensions-and-nanoemulsions-of-citral-in-combination-with-heat-or-pulsed-electric-fields
#4
E Pagán, D Berdejo, L Espina, D García-Gonzalo, R Pagán
The application of essential oils in form of nanoemulsions has been proposed as a method to improve their solubility in aqueous solutions, and hence their antimicrobial activity. The objective of this study was to evaluate the antimicrobial activity of citral, applied directly or in combined treatments with heat or pulsed electric fields (PEF), as a function of the inoculation procedure assayed: (i) a simple, vigorous shaking method by vortex agitation (suspension of citral; s-citral) or (ii) the previous preparation of nanoemulsions by the emulsion phase inversion (EPI) method (nanoemulsion of citral; n-citral)...
October 26, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29051644/chemical-compositions-antioxidant-and-antimicrobial-properties-of-ziziphora-clinopodioides-lam-essential-oils-collected-from-different-parts-of-iran
#5
Yasser Shahbazi
The aims of the present study were to investigate chemical compositions, antioxidant and antimicrobial properties of Ziziphora clinopodioides essential oils (ZEOs) collected from four provinces in western Iran (Ilam, Lorestan, Kermanshah and Kurdestan). Carvacrol was the most abundant constituent in the flower, stem and leaf oil samples of Ilam, Lorestan and Kermanshah regions by 73.12-74.29%, 66.47-66.89% and 65.11-65.32%, respectively. The most abundant components in Kurdestan sample were thymol (55.32-55...
October 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/29044827/identifying-the-mechanism-of-escherichia-coli-o157-h7-survival-by-the-addition-of-salt-in-the-treatment-with-organic-acids
#6
Young-Min Bae, Jae-Hyun Yoon, Ji-Yeon Kim, Sun-Young Lee
AIM: In this study, the effects of addition of salt to treatment with acids (one of several organic acids and salt in various solutions including rich or minimal broth, buffer, or distilled water) on the reduction of Escherichia coli O157:H7 were investigated. The protein expression profiles corresponding to acid stress (acetic acid) with or without salt addition were studied using a comparative proteomic analysis of E. coli O157:H7. METHODS AND RESULTS: When acetic, lactic, or propionic acid was combined with 3% NaCl, mutually antagonistic effects of acid and salt on viability of E...
October 17, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29035438/high-throughput-screening-of-escherichia-coli-o157-h7-pathogenic-genes-via-pathway-enrichment-and-operon-analysis
#7
Guangxin Yuan, Jia Fu, Liping An, Yan Zhuang, Yu Wang, Yunfeng Song, Zijing Qiao, Xiaolong Wang, Yufei Tian, Xiao Han, Peige Du, Guangyu Xu
BACKGROUND: About thirty thousand people globally die every day from infectious diarrhea, mostly caused by pathogenic Escherichia coli (E. coli) O157:H7. METHODS: In order to search for clinical diagnostic biomarkers and novel drug targets for infectious diarrhea, we used a bibliometric method to collect pathogenic genes of E. coli O157:H7 and performed a functional analysis of the important pathogenic genes by pathway enrichment and operon analysis. RESULTS: We found 364 pathogenic genes which may be involved in infection with E...
October 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28975041/escherichia-coli-o157-h7-suppresses-host-autophagy-and-promotes-epithelial-adhesion-via-tir-mediated-and-camp-independent-activation-of-protein-kinase-a
#8
Yansong Xue, Min Du, Haiqing Sheng, Carolyn J Hovde, Mei-Jun Zhu
Autophagy is a pivotal innate immune response that not only degrades cytosolic components, but also serves as one of the critical antimicrobial mechanisms eliminating intracellular pathogens. However, its role in host defense against extracellular pathogens is largely unknown. Here we showed that E. coli O157:H7 altered autophagy to evade host defense and facilitate adhesion. Enhancing host cell autophagy with tumor necrosis factor (TNF), host starvation or rapamycin reduced the adherence of E. coli O157:H7 to HT-29 cells...
2017: Cell Death Discovery
https://www.readbyqxmd.com/read/28884271/the-effect-of-anaerobic-digestion-and-storage-on-indicator-microorganisms-in-swine-and-dairy-manure
#9
Annamaria Costa, Claudia Gusmara, Davide Gardoni, Mauro Zaninelli, Fulvia Tambone, Vittorio Sala, Marcella Guarino
The aim of this experimental study was to evaluate the influence of anaerobic digestion and storage on indicator microorganisms in swine and dairy excreta. Samples were collected every 90 days for 15 months at eight farms, four pig, and four dairy farms, four of them having a biogas plant. Moreover, to evaluate storage effects on samples, 20 l of manure and slurry taken at each farm (digested manure only in farms with a biogas plant) were stored in a controlled climatic chamber at 18 °C, for 6 months...
November 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28865862/rapid-and-simultaneous-quantification-of-viable-escherichia-coli-o157-h7-and-salmonella-spp-in-milk-through-multiplex-real-time-pcr
#10
Baoqing Zhou, Taobo Liang, Zhongxu Zhan, Rui Liu, Fan Li, Hengyi Xu
Escherichia coli O157:H7 and Salmonella spp. in milk are 2 common pathogens that cause foodborne diseases. An accurate, rapid, specific method has been developed for the simultaneous detection of viable E. coli O157:H7 and Salmonella spp. in milk. Two specific genes, namely, fliC from E. coli O157:H7 and invA from Salmonella spp., were selected to design primers and probes. A combined treatment containing sodium deoxycholate (SDO) and propidium monoazide (PMA) was applied to detect viable E. coli O157:H7 and Salmonella spp...
November 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28852487/rare-genetic-variants-in-shiga-toxin-associated-haemolytic-uraemic-syndrome-genetic-analysis-prior-to-transplantation-is-essential
#11
Frances Dowen, Katrina Wood, Alison L Brown, Jennifer Palfrey, David Kavanagh, Vicky Brocklebank
We present a case of haemolytic uraemic syndrome (HUS) in a 16-year-old female with serological evidence of acute Escherichia coli O157:H7 infection. She progressed to established renal failure and received a deceased donor kidney transplant. Shiga toxin-associated HUS (STEC-HUS) does not recur following renal transplantation, but unexpectedly this patient did experience rapid and severe HUS recurrence. She responded to treatment with the terminal complement inhibitor eculizumab and subsequent genetic analysis revealed a rare variant in a complement gene...
August 2017: Clinical Kidney Journal
https://www.readbyqxmd.com/read/28843125/combination-of-essential-oil-compounds-and-phenolic-acids-against-escherichia-coli-o157-h7-in-vitro-and-in-dry-fermented-sausage-production
#12
Natan V B Meira, Richard A Holley, Keliani Bordin, Renata E F de Macedo, Fernando B Luciano
Escherichia coli O157:H7 is a foodborne pathogen that causes hemorrhagic colitis and hemolytic uremic syndrome. The low dose of infection and severity of the disease represent a concern to public health. Natural compounds have been widely applied as food additives to replace synthetic preservatives. The aim of this study was to determine the efficiency of essential oil compounds (EOCs) in combination with phenolic acids (PA) in vitro and in dry-fermented sausage production. Minimum Inhibitory Concentration (MIC) and Fractional Inhibitory Concentration index (FICindex) were determined for a 5-strain mixture of E...
August 16, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28843119/biofilm-of-escherichia-coli-o157-h7-on-cantaloupe-surface-is-resistant-to-lauroyl-arginate-ethyl-and-sodium-hypochlorite
#13
Yezhi Fu, Amanda J Deering, Arun K Bhunia, Yuan Yao
Biofilms formed by Escherichia coli O157:H7 on cantaloupe rind were characterized in this study. Cantaloupe rind pieces inoculated with E. coli O157:H7 B6-914 was sampled after 2, 12, and 24h incubation for imaging with cryo-scanning electron microscopy (Cryo-SEM) or treating with lauroyl arginate ethyl (LAE) or sodium hypochlorite (SHC). Cryo-SEM images showed that E. coli O157:H7 formed a biofilm within 12h on the rind surface. For rind samples treated with LAE or SHC, the residual cell counts were significantly different (p<0...
August 16, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28814799/on-mechanism-behind-uv-a-light-enhanced-antibacterial-activity-of-gallic-acid-and-propyl-gallate-against-escherichia-coli-o157-h7
#14
Qingyang Wang, Erick Falcao de Oliveira, Solmaz Alborzi, Luis J Bastarrachea, Rohan V Tikekar
Possible mechanisms behind the enhanced antimicrobial activity of gallic acid (GA) and its ester propyl gallate (PG) in the presence of UV-A light against Escherichia coli O157:H7 were investigated. GA by itself is a mild antimicrobial and has a pro-oxidant ability. We found that the presence of UV-A light increases the uptake of GA by the bacteria. Once GA is internalized, the interaction between GA and UV-A induces intracellular ROS formation, leading to oxidative damage. Concurrently, GA + UV-A also inhibits the activity of superoxide dismutase (SOD), magnifying the imbalance of redox status of E...
August 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28805884/the-effects-of-monensin-in-diets-fed-to-finishing-beef-steers-and-heifers-on-growth-performance-and-fecal-shedding-of-escherichia-coli-o157-h7
#15
K E Hales, J E Wells, E D Berry, N Kalchayanand, J L Bono, M Kim
Two experiments were conducted to study the effects of monensin dose on growth performance and O157:H7 shedding in finishing beef cattle. In Exp. 1, 198 heifers (298 ± 1.1 kg BW) were allocated to 1 of 2 treatments consisting of 1) 200 mg/heifer daily of monensin and 2) 400 mg/heifer daily of monensin and fed for 151 d. In Exp. 2, 199 steers (430 ± 1.9 kg BW) were stratified by BW and allocated to 1 of 2 treatments consisting of 1) 0 mg/steer daily of monensin and 2) 400 mg/steer daily of monensin and fed for 128 d...
August 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28805457/selection-of-surrogate-bacteria-for-use-in-food-safety-challenge-studies-a-review
#16
REVIEW
Mengyi Hu, Joshua B Gurtler
Nonpathogenic surrogate bacteria are prevalently used in a variety of food challenge studies in place of foodborne pathogens such as Listeria monocytogenes, Salmonella, Escherichia coli O157:H7, and Clostridium botulinum because of safety and sanitary concerns. Surrogate bacteria should have growth characteristics and/or inactivation kinetics similar to those of target pathogens under given conditions in challenge studies. It is of great importance to carefully select and validate potential surrogate bacteria when verifying microbial inactivation processes...
September 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28741927/rapid-antibiotic-susceptibility-testing-of-uropathogenic-e-coli-by-tracking-submicron-scale-motion-of-single-bacterial-cells
#17
Karan Syal, Simon Shen, Yunze Yang, Shaopeng Wang, Shelley E Haydel, Nongjian Tao
To combat antibiotic resistance, a rapid antibiotic susceptibility testing (AST) technology that can identify resistant infections at disease onset is required. Current clinical AST technologies take 1-3 days, which is often too slow for accurate treatment. Here we demonstrate a rapid AST method by tracking sub-μm scale bacterial motion with an optical imaging and tracking technique. We apply the method to clinically relevant bacterial pathogens, Escherichia coli O157: H7 and uropathogenic E. coli (UPEC) loosely tethered to a glass surface...
August 25, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28648298/slightly-acidic-electrolyzed-water-combined-with-chemical-and-physical-treatments-to-decontaminate-bacteria-on-fresh-fruits
#18
Charles Nkufi Tango, Imran Khan, Paul-François Ngnitcho Kounkeu, Rubab Momna, Mohammad Shakhawat Hussain, Deog-Hwan Oh
Effect of sequential combination of slightly acidic electrolyzed water (SAEW) with chemical and physical treatments on bacterial decontamination on fruits was investigated in this study. Effect of treatments on microbial and sensory quality was also analyzed after subsequent storage at 4 °C and room temperature (RT, 23 ± 0.15 °C). Whole apple and tomato fruits were inoculated with cocktail strains of Escherichia coli O157:H7 and Listeria monocytogenes. Uninoculated and inoculated fruits were washed first with distilled water (DW), calcium oxide (CaO), fumaric acid (FA), and SAEW at RT for 3 min...
October 2017: Food Microbiology
https://www.readbyqxmd.com/read/28630239/an-unusual-case-of-escherichia-coli-o157-h7-infection-with-pseudomembranous-colitis-like-lesions-associated-with-haemolytic-uraemic-syndrome-and-neurological-sequelae
#19
James Kennedy, Lauren Simmonds, Robert Orme, Warren Doherty
A 75-year-old man was admitted with abdominal pain and fresh rectal bleeding. Significantly, he had no risk factors for Clostridium difficile infection. An abdominal CT demonstrated colonic thickening, and flexible sigmoidoscopy identified pseudomembranous colitis-like lesions. After initial treatment as C. difficile colitis, a stool sample revealed Escherichia coli O157:H7 infection. Antibiotic therapy was stopped due to the risk of lysis-mediated toxin release, but unfortunately, the patient continued to deteriorate...
June 18, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28610446/impacts-of-pulsed-electric-field-and-heat-treatment-on-quality-and-sensory-properties-and-microbial-inactivation-of-pomegranate-juice
#20
Gulsun A Evrendilek
Synergistic effects of pulsed electric field+mild heat on quality properties of pomegranate juice were modeled using the best-fit multiple (non-) linear regression models with inactivation kinetics parameters of Escherichia coli O157:H7 and Staphylococcus aureus. No significant difference was detected between the control and the treated samples in terms of pH; °Brix; total antioxidant capacity; total monomeric anthocyanin content; total ascorbic acid concentration; and the sensory properties of flavor, taste, aftertaste, and overall acceptance ( p > 0...
January 1, 2017: Food Science and Technology International, Ciencia y Tecnología de Los Alimentos Internacional
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