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Escherichia Coli O157 H7 treatment

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https://www.readbyqxmd.com/read/27889137/microbial-decontamination-of-onion-powder-using-microwave-powered-cold-plasma-treatments
#1
Jung Eun Kim, Yeong Ji Oh, Mee Yeon Won, Kwang-Sik Lee, Sea C Min
The effects of microwave-integrated cold plasma (CP) treatments against spores of Bacillus cereus and Aspergillus brasiliensis and Escherichia coli O157:H7 on onion powder were investigated. The growth of B. cereus, A. brasiliensis, and E. coli O157:H7 in the treated onion powder was assessed during storage at 4 and 25 °C, along with the physicochemical and sensory properties of the powder. Onion powder inoculated with B. cereus was treated with CP using helium as a plasma-forming gas, with simultaneous exposure to low microwave density at 170 mW m(-2) or high microwave density at 250 mW m(-2)...
April 2017: Food Microbiology
https://www.readbyqxmd.com/read/27870549/evaluation-of-bacteriophage-application-to-cattle-in-lairage-at-beef-processing-plants-to-reduce-escherichia-coli-o157-h7-prevalence-on-hides-and-carcasses
#2
Terrance M Arthur, Norasak Kalchayanand, Getahun E Agga, Tommy L Wheeler, Mohammad Koohmaraie
Escherichia coli O157:H7 is a major food safety concern for the beef industry. Several studies have provided evidence that cattle hides are the main source of beef carcass contamination during processing and that reductions in the E. coli O157:H7 load on the hides of cattle entering processing facilities will lead to reductions in carcass contamination. Bacteriophages have been proposed as a novel preharvest antimicrobial intervention to reduce the levels of E. coli O157:H7 on cattle hides. The objective of this study was to evaluate a commercialized phage application administered in the lairage area of commercial beef processing plants for the ability to reduce E...
November 21, 2016: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/27858378/label-free-3d-ag-nanoflower-based-electrochemical-immunosensor-for-the-detection-of-escherichia-coli-o157-h7-pathogens
#3
He Huang, Minghuan Liu, Xiangsheng Wang, Wenjie Zhang, Da-Peng Yang, Lianhua Cui, Xiansong Wang
It is highly desirable to develop a rapid and simple method to detect pathogens. Combining nanomaterials with electrochemical techniques is an efficient way for pathogen detection. Herein, a novel 3D Ag nanoflower was prepared via a biomineralization method by using bovine serum albumin (BSA) as a template. It was adopted as a sensing interface to construct an electrochemical bacteria immunosensor for the rapid detection of foodborne pathogens Escherichia coli (E. coli) O157:H7. Bacterial antibody was immobilized onto the surface of Ag nanoflowers through covalent conjugation...
December 2016: Nanoscale Research Letters
https://www.readbyqxmd.com/read/27794249/cross-protection-between-controlled-acid-adaptation-and-thermal-inactivation-for-48-escherichia-coli-strains
#4
Leticia Ungaretti Haberbeck, Xiang Wang, Chris Michiels, Frank Devlieghere, Mieke Uyttendaele, Annemie H Geeraerd
Given the importance of pH reduction and thermal treatment in food processing and food preservation strategies, the cross-protection between acid adaptation and subsequent thermal inactivation for 48 Escherichia coli strains was investigated. Those strains were selected among 188 E. coli strains according to their odds of growth under low pH conditions as determined by Haberbeck et al. (2015) [Haberbeck, L.U., Oliveira, R.C., Vivijs, B., Wenseleers, T., Aertsen, A., Michiels, C., Geeraerd, A.H., 2015. Variability in growth/no growth boundaries of 188 different Escherichia coli strains reveals that approximately 75% have a higher growth probability under low pH conditions than E...
October 11, 2016: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/27780962/combined-treatment-on-the-inactivation-of-naturally-existing-bacteria-and-escherichia-coli-o157-h7-inoculated-on-fresh-cut-kale
#5
Ji Hoon Kang, Kyung Bin Song
An aqueous chlorine dioxide (ClO₂) treatment combined with highly activated calcium oxide (CaO) and mild heat inactivated naturally existing bacteria and Escherichia coli O157:H7 inoculated on fresh-cut kale. Kale samples were treated with different concentrations of ClO₂ (10, 30, and 50 ppm), CaO (0.01, 0.05, 0.1, and 0.2%), and mild heat (25, 45, 55, and 65°C) as well with combinations of 30 or 50 ppm ClO₂ and 0.2% CaO at 55°C for 3 min. An increasing concentration of ClO₂ and CaO significantly reduced the microbial population compared to the control...
October 25, 2016: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27744210/active-edible-coating-and-%C3%AE-irradiation-as-cold-combined-treatments-to-assure-the-safety-of-broccoli-florets-brassica-oleracea-l
#6
Yosra Ben-Fadhel, Sabrina Saltaji, Mohamed Ali Khlifi, Stephane Salmieri, Khanh Dang Vu, Monique Lacroix
The antimicrobial activity of essential oils (EOs), organic acid (OA) salts and natamycin, a natural antifungal produced during fermentation by the bacterium Streptomyces natalensis, was assessed against four pathogens (Escherichia coli O157:H7, Listeria monocytogenes, Salmonella Typhimurium and Aspergillus niger). The Minimum Inhibitory Concentration (MIC) of each antimicrobial (AM) was assessed to determine their efficiency on tested microbial species in order to select the most efficient. Then, the interactions between different antimicrobial compounds showing the lowest MIC were determined by the checkerboard method...
October 11, 2016: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/27722576/co-loaded-proteinase-k-thyme-oil-liposomes-for-inactivation-of-escherichia-coli-o157-h7-biofilms-on-cucumber
#7
Haiying Cui, Cuixia Ma, Lin Lin
E. coli O157:H7 is a bacterium frequently found on vegetable surfaces, such as cucumber, that can pose a significant threat to consumers. In the present work, proteinase K (PK) and thyme oil (TO) were used to inactivate E. coli O157:H7 biofilms. Both PK and TO were encapsulated in liposomes to improve their chemical stability and to allow for a controlled release. The optimal PK/TO-loaded liposomes (particle size of 170.4 nm, polydispersity index of 0.309, zeta potential of -29.8 mV and entrapment efficiency of 33...
September 14, 2016: Food & Function
https://www.readbyqxmd.com/read/27718408/flexible-thin-layer-plasma-inactivation-of-bacteria-and-mold-survival-in-beef-jerky-packaging-and-its-effects-on-the-meat-s-physicochemical-properties
#8
Hae In Yong, Haelim Lee, Sanghoo Park, Jooyoung Park, Wonho Choe, Samooel Jung, Cheorun Jo
The aims of the present study were to examine the use of a flexible thin-layer plasma system in inactivating bacteria and mold on beef jerky in a commercial package and to evaluate the physicochemical changes of the jerky. After plasma treatment for 10min, Escherichia coli O157:H7, Listeria monocytogenes, Salmonella Typhimurium, and Aspergillus flavus populations on the beef jerky were reduced by approximately 2 to 3Log CFU/g. No significant changes in metmyoglobin content, shear force, and myofibrillar fragmentation index were found in the plasma-treated beef jerky...
September 30, 2016: Meat Science
https://www.readbyqxmd.com/read/27713208/antimicrobial-activity-of-flaxseed-meal-extract-against-escherichia-coli-o157-h7-and-staphylococcus-aureus-inoculated-on-red-mustard
#9
Hyeon-Jeong Son, Kyung Bin Song
This study examined the antimicrobial activity of flaxseed meal extract (FME) against Staphylococcus aureus and Escherichia coli O157:H7 inoculated on red mustard. With the treatment of 0.7% FME for 3 min, the reduction levels of S. aureus and E. coli O157:H7 populations were 1.23 and 1.83 log CFU/g, respectively. In addition, the combined treatment of 0.7% FME at 50°C for 3 min reduced the populations of the pathogenic bacteria by 2.28 and 2.41 log CFU/g, respectively. Color and vitamin C content were not significantly different between treatments...
October 6, 2016: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27697158/pulsed-light-inactivation-of-murine-norovirus-tulane-virus-escherichia-coli-o157-h7-and-salmonella-in-suspension-and-on-berry-surfaces
#10
Yaoxin Huang, Mu Ye, Xinang Cao, Haiqiang Chen
Pulsed light (PL) inactivation of two human norovirus (HuNoV) surrogates, murine norovirus (MNV-1) and Tulane virus (TV), and two bacterial pathogens, Escherichia coli O157:H7 and Salmonella, were evaluated. The viruses and bacteria were suspended in phosphate buffered saline (PBS) to final populations of ∼6 log PFU/mL and ∼6 log CFU/mL, respectively. Both viral and bacterial suspensions were then irradiated by PL for different durations and the reductions of each microorganisms were determined. MNV-1 and TV were significantly (P < 0...
February 2017: Food Microbiology
https://www.readbyqxmd.com/read/27625996/from-in-silico-protein-epitope-density-prediction-to-testing-escherichia-coli-o157-h7-vaccine-candidates-in-a-murine-model-of-colonization
#11
Daniel Tapia, Brittany N Ross, Anjana Kalita, Mridul Kalita, Christopher L Hatcher, Laura A Muruato, Alfredo G Torres
Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a leading cause of foodborne illnesses worldwide and is a common serotype linked to hemorrhagic colitis and an important cause of hemolytic uremic syndrome (HUS). Treatment of EHEC O157:H7 infections is complicated, as antibiotics can exacerbate Shiga toxin (Stx) production and lead to more severe symptoms including HUS. To date, no vaccines have been approved for human use, exposing a void in both treatment and prevention of EHEC O157:H7 infections. Previously, our lab has shown success in identifying novel vaccine candidates via bio- and immunoinformatics approaches, which are capable of reducing bacterial colonization in an in vivo model of intestinal colonization...
2016: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/27611358/combination-of-cymbopogon-citratus-and-allium-cepa-essential-oils-increased-antibacterial-activity-in-leafy-vegetables
#12
Luis A Ortega-Ramirez, Brenda A Silva-Espinoza, Irasema Vargas-Arispuro, Gustavo A Gonzalez-Aguilar, M Reynaldo Cruz-Valenzuela, Filomena Nazzaro, J Fernando Ayala-Zavala
BACKGROUND: Cymbopogon citratus and Allium cepa essential oils (EOs) are rich in terpenes and sulfur compounds respectively, both with antibacterial activity and different cell targets, supporting the idea that their combination can increase their efficacy. RESULTS: Major constituents of C. citratus were geranial and neral, while A. cepa presented dipropyl disulfide and dipropyl trisulfide. Cymbopogon citratus and A. cepa EOs inhibited the in vitro growth of Escherichia coli O157:H7 (minimal inhibitory concentrations of 2...
September 9, 2016: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/27578754/new-insights-into-the-formation-of-viable-but-nonculturable-escherichia-coli-o157-h7-induced-by-high-pressure-co2
#13
Feng Zhao, Yongtao Wang, Haoran An, Yanling Hao, Xiaosong Hu, Xiaojun Liao
UNLABELLED: The formation of viable but nonculturable (VBNC) Escherichia coli O157:H7 induced by high-pressure CO2 (HPCD) was investigated using RNA sequencing (RNA-Seq) transcriptomics and isobaric tag for relative and absolute quantitation (iTRAQ) proteomic methods. The analyses revealed that 97 genes and 56 proteins were significantly changed upon VBNC state entry. Genes and proteins related to membrane transport, central metabolisms, DNA replication, and cell division were mainly downregulated in the VBNC cells...
2016: MBio
https://www.readbyqxmd.com/read/27570746/mutation-of-the-enterohemorrhagic-escherichia-coli-core-lps-biosynthesis-enzyme-rfad-confers-hypersusceptibility-to-host-intestinal-innate-immunity-in-vivo
#14
Cheng-Ju Kuo, Jenn-Wei Chen, Hao-Chieh Chiu, Ching-Hao Teng, Tai-I Hsu, Pei-Jung Lu, Wan-Jr Syu, Sin-Tian Wang, Ting-Chen Chou, Chang-Shi Chen
Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is an important foodborne pathogen causing severe diseases in humans worldwide. Currently, there is no specific treatment available for EHEC infection and the use of conventional antibiotics is contraindicated. Therefore, identification of potential therapeutic targets and development of effective measures to control and treat EHEC infection are needed. Lipopolysaccharides (LPS) are surface glycolipids found on the outer membrane of gram-negative bacteria, including EHEC, and LPS biosynthesis has long been considered as potential anti-bacterial target...
2016: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/27569378/sanitizing-radish-seeds-by-simultaneous-treatments-with-gaseous-chlorine-dioxide-high-relative-humidity-and-mild-heat
#15
Jihyun Bang, Moonhak Choi, Hyeri Son, Larry R Beuchat, Yoonsook Kim, Hoikyung Kim, Jee-Hoon Ryu
Sanitizing radish seeds intended for edible sprout production was achieved by applying simultaneous treatments with gaseous chlorine dioxide (ClO2), high relative humidity (RH, 100%), and mild heat (55°C). Gaseous ClO2 was produced from aqueous ClO2 (0.66ml) by mixing sulfuric acid (5% w/v) with sodium chlorite (10 mg/mL) in a sealed container (1.8L). Greater amounts of gaseous ClO2 were measured at 23% RH (144ppm after 6h) than at 100% RH (66ppm after 6h); however, the lethal activity of gaseous ClO2 against naturally occurring mesophilic aerobic bacteria (MAB) on radish seeds was significantly enhanced at 100% RH...
November 21, 2016: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/27562348/dielectric-barrier-discharge-atmospheric-cold-plasma-inhibits-escherichia-coli-o157-h7-salmonella-listeria-monocytogenes-and-tulane-virus-in-romaine-lettuce
#16
Sea C Min, Si Hyeon Roh, Brendan A Niemira, Joseph E Sites, Glenn Boyd, Alison Lacombe
The present study investigated the effects of dielectric barrier discharge atmospheric cold plasma (DACP) treatment on the inactivation of Escherichia coli O157:H7, Salmonella, Listeria monocytogenes, and Tulane virus (TV) on Romaine lettuce, assessing the influences of moisture vaporization, modified atmospheric packaging (MAP), and post-treatment storage on the inactivation of these pathogens. Romaine lettuce was inoculated with E. coli O157:H7, Salmonella, L. monocytogenes (~6logCFU/g lettuce), or TV (~2logPFU/g lettuce) and packaged in either a Petri dish (diameter: 150mm, height: 15mm) or a Nylon/polyethylene pouch (152×254mm) with and without moisture vaporization...
November 21, 2016: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/27554151/influence-of-water-activity-on-inactivation-of-escherichia-coli-o157-h7-salmonella-typhimurium-and-listeria-monocytogenes-in-peanut-butter-by-microwave-heating
#17
Won-Jae Song, Dong-Hyun Kang
This study evaluated the efficacy of a 915 MHz microwave with 3 different electric power levels to inactivate three pathogens in peanut butter with different aw. Peanut butter inoculated with Escherichia coli O157:H7, Salmonella enterica serovar Typhimurium and Listeria monocytogenes (0.3, 0.4, and 0.5 aw) were treated with a 915 MHz microwave with 2, 4, and 6 kW for up to 5 min. Six kW 915 MHz microwave treatment for 5 min reduced these three pathogens by 1.97 to >5.17 log CFU/g. Four kW 915 MHz microwave processing for 5 min reduced these pathogens by 0...
December 2016: Food Microbiology
https://www.readbyqxmd.com/read/27497118/effect-of-hydrogen-peroxide-in-combination-with-minimal-thermal-treatment-for-reducing-bacterial-populations-on-cantaloupe-rind-surfaces-and-transfer-to-fresh-cut-pieces
#18
Dike O Ukuku, Sudarsan Mukhopadhyay, David Geveke, Modesto Olanya, Brendan Niemira
Surface structure and biochemical characteristics of bacteria and produce play a major role in how and where bacteria attach, complicating decontamination treatments. Whole cantaloupe rind surfaces were inoculated with Salmonella, Escherichia coli O157:H7, and Listeria monocytogenes at 10(7) CFU/ml. Average population size of Salmonella, Escherichia coli O157:H7, and L. monocytogenes recovered after surface inoculation was 4.8 ± 0.12, 5.1 ± 0.14, and 3.6 ± 0.13 log CFU/cm(2), respectively. Inoculated melons were stored at 5 and 22°C for 7 days before washing treatment interventions...
August 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/27484251/quality-attributes-and-microbial-survival-on-whole-cantaloupes-with-antimicrobial-coatings-containing-chitosan-lauric-arginate-cinnamon-oil-and-ethylenediaminetetraacetic-acid
#19
Qiumin Ma, Yue Zhang, Faith Critzer, P Michael Davidson, Qixin Zhong
Cantaloupes are susceptible to microbiological contamination in pre- or postharvest environments. Novel intervention strategies, such as antimicrobial coatings, are needed to improve the microbiological safety of cantaloupes. The objective of this study was to prepare whole cantaloupes coated with mixtures containing chitosan, lauric arginate (LAE), cinnamon oil (CO), and ethylenediaminetetraacetic acid (EDTA) and determine survival characteristics of inoculated foodborne pathogens during storage as well as cantaloupe quality attributes...
October 17, 2016: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/27462311/whole-transcriptome-analysis-of-verocytotoxigenic-escherichia-coli-o157-h7-sakai-suggests-plant-species-specific-metabolic-responses-on-exposure-to-spinach-and-lettuce-extracts
#20
Louise Crozier, Pete E Hedley, Jenny Morris, Carol Wagstaff, Simon C Andrews, Ian Toth, Robert W Jackson, Nicola J Holden
Verocytotoxigenic Escherichia coli (VTEC) can contaminate crop plants, potentially using them as secondary hosts, which can lead to food-borne infection. Currently, little is known about the influence of the specific plant species on the success of bacterial colonization. As such, we compared the ability of the VTEC strain, E. coli O157:H7 'Sakai,' to colonize the roots and leaves of four leafy vegetables: spinach (Spinacia oleracea), lettuce (Lactuca sativa), vining green pea (Pisum sativum), and prickly lettuce (Lactuca serriola), a wild relative of domesticated lettuce...
2016: Frontiers in Microbiology
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