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Escherichia Coli O157 H7 treatment

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https://www.readbyqxmd.com/read/28805884/the-effects-of-monensin-in-diets-fed-to-finishing-beef-steers-and-heifers-on-growth-performance-and-fecal-shedding-of-escherichia-coli-o157-h7
#1
K E Hales, J E Wells, E D Berry, N Kalchayanand, J L Bono, M Kim
Two experiments were conducted to study the effects of monensin dose on growth performance and O157:H7 shedding in finishing beef cattle. In Exp. 1, 198 heifers (298 ± 1.1 kg BW) were allocated to 1 of 2 treatments consisting of 1) 200 mg/heifer daily of monensin and 2) 400 mg/heifer daily of monensin and fed for 151 d. In Exp. 2, 199 steers (430 ± 1.9 kg BW) were stratified by BW and allocated to 1 of 2 treatments consisting of 1) 0 mg/steer daily of monensin and 2) 400 mg/steer daily of monensin and fed for 128 d...
August 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28805457/selection-of-surrogate-bacteria-for-use-in-food-safety-challenge-studies-a-review
#2
Mengyi Hu, Joshua B Gurtler
Nonpathogenic surrogate bacteria are prevalently used in a variety of food challenge studies in place of foodborne pathogens such as Listeria monocytogenes, Salmonella, Escherichia coli O157:H7, and Clostridium botulinum because of safety and sanitary concerns. Surrogate bacteria should have growth characteristics and/or inactivation kinetics similar to those of target pathogens under given conditions in challenge studies. It is of great importance to carefully select and validate potential surrogate bacteria when verifying microbial inactivation processes...
August 14, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28741927/rapid-antibiotic-susceptibility-testing-of-uropathogenic-e-coli-by-tracking-sub-micron-scale-motion-of-single-bacterial-cells
#3
Karan Syal, Simon Shen, Yunze Yang, Shaopeng Wang, Shelley E Haydel, Nongjian Tao
To combat antibiotic resistance, a rapid antibiotic susceptibility testing (AST) technology that can identify resistant infections at disease onset is required. Current clinical AST technologies take 1-3 days, which is often too slow for accurate treatment. Here we demonstrate a rapid AST method by tracking sub-µm scale bacterial motion with an optical imaging and tracking technique. We apply the method to clinically relevant bacterial pathogens, Escherichia coli O157: H7 and uropathogenic E. coli (UPEC) loosely tethered to a glass surface...
July 25, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28648298/slightly-acidic-electrolyzed-water-combined-with-chemical-and-physical-treatments-to-decontaminate-bacteria-on-fresh-fruits
#4
Charles Nkufi Tango, Imran Khan, Paul-François Ngnitcho Kounkeu, Rubab Momna, Mohammad Shakhawat Hussain, Deog-Hwan Oh
Effect of sequential combination of slightly acidic electrolyzed water (SAEW) with chemical and physical treatments on bacterial decontamination on fruits was investigated in this study. Effect of treatments on microbial and sensory quality was also analyzed after subsequent storage at 4 °C and room temperature (RT, 23 ± 0.15 °C). Whole apple and tomato fruits were inoculated with cocktail strains of Escherichia coli O157:H7 and Listeria monocytogenes. Uninoculated and inoculated fruits were washed first with distilled water (DW), calcium oxide (CaO), fumaric acid (FA), and SAEW at RT for 3 min...
October 2017: Food Microbiology
https://www.readbyqxmd.com/read/28630239/an-unusual-case-of-escherichia-coli-o157-h7-infection-with-pseudomembranous-colitis-like-lesions-associated-with-haemolytic-uraemic-syndrome-and-neurological-sequelae
#5
James Kennedy, Lauren Simmonds, Robert Orme, Warren Doherty
A 75-year-old man was admitted with abdominal pain and fresh rectal bleeding. Significantly, he had no risk factors for Clostridium difficile infection. An abdominal CT demonstrated colonic thickening, and flexible sigmoidoscopy identified pseudomembranous colitis-like lesions. After initial treatment as C. difficile colitis, a stool sample revealed Escherichia coli O157:H7 infection. Antibiotic therapy was stopped due to the risk of lysis-mediated toxin release, but unfortunately, the patient continued to deteriorate...
June 18, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28610446/impacts-of-pulsed-electric-field-and-heat-treatment-on-quality-and-sensory-properties-and-microbial-inactivation-of-pomegranate-juice
#6
Gulsun A Evrendilek
Synergistic effects of pulsed electric field+mild heat on quality properties of pomegranate juice were modeled using the best-fit multiple (non-) linear regression models with inactivation kinetics parameters of Escherichia coli O157:H7 and Staphylococcus aureus. No significant difference was detected between the control and the treated samples in terms of pH; °Brix; total antioxidant capacity; total monomeric anthocyanin content; total ascorbic acid concentration; and the sensory properties of flavor, taste, aftertaste, and overall acceptance ( p > 0...
January 1, 2017: Food Science and Technology International, Ciencia y Tecnología de Los Alimentos Internacional
https://www.readbyqxmd.com/read/28607018/antibiotic-mediated-modulations-of-outer-membrane-vesicles-in-enterohemorrhagic-escherichia-coli-o104-h4-and-o157-h7
#7
Andreas Bauwens, Lisa Kunsmann, Helge Karch, Alexander Mellmann, Martina Bielaszewska
Ciprofloxacin, meropenem, fosfomycin, and polymyxin B strongly increase production of outer membrane vesicles (OMVs) in Escherichia coli O104:H4 and O157:H7. Ciprofloxacin also upregulates OMV-associated Shiga toxin 2a, the major virulence factor of these pathogens, whereas the other antibiotics increase OMV production without the toxin. Both these effects might worsen the clinical outcome of infections caused by Shiga toxin-producing E. coli Our data support the existing recommendations to avoid antibiotics for treatment of these infections...
June 12, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28596790/high-doses-of-halotolerant-gut-indigenous-lactobacillus-plantarum-reduce-cultivable-lactobacilli-in-newborn-calves-without-increasing-its-species-abundance
#8
Alexander Rodriguez-Palacios, Henry R Staempfli, J Scott Weese
To elucidate the ecological effect of high oral doses of halotolerant (resistant to table salt) indigenous-gut bacteria on other commensals early in life, we conducted a culture-based study to quantify the effect of intestinal Lactobacillus plantarum strain of bovine origin (with remarkable aerobic growth capabilities and inhibitory activity against Escherichia coli O157:H7 and F5) on clinical health and gut lactobacilli/coliforms in newborn calves. In a double-blind placebo-randomized trial twelve colostrum-fed calves, consecutively born at a farm, were fed L...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/28578053/bactericidal-effect-of-266-to-279nm-wavelength-uvc-leds-for-inactivation-of-gram-positive-and-gram-negative-foodborne-pathogenic-bacteria-and-yeasts
#9
Do-Kyun Kim, Soo-Ji Kim, Dong-Hyun Kang
Recently, UVC-LED technology has been validated as an alternative to irradiation with conventional mercury UV lamps. In this study, we sought to determine primary factors affecting reduction trends shown in several microorganisms. Four major foodborne pathogens (Escherichia coli O157:H7, Salmonella spp. Listeria monocytogenes, Staphylococcus aureus) and spoilage yeasts (Saccharomyces pastorianus, Pichia membranaefaciens), important to the brewing industry, were inoculated onto selective and non-selective media in order to investigate reduction tendencies at 4 different peak wavelengths (266 to 279nm)...
July 2017: Food Research International
https://www.readbyqxmd.com/read/28573247/in-vitro-study-of-concentration-effect-and-time-course-pattern-of-white-alum-on-escherichia-coli-o157-h7-growth
#10
Reza Shahriari, Saeed Salari, Saeed Shahriari
BACKGROUND: Nowadays, the demand for antibacterial fabrics has increased. White alum is used for oral aphthous ulcers treatment in traditional medicine of Sistan city, Sistan and Baluchistan Province, Iran, and also as a flocculent for water purification. This study is aimed to evaluate the effect of concentration and time on antibacterial activity of white alum on Escherichia coli O157:H7. MATERIALS AND METHODS: 0.25, 0.5, 1 and 2% concentrations of white alum were added to 10(8) CFU of Escherichia coli O157:H7...
2017: African Journal of Traditional, Complementary, and Alternative Medicines: AJTCAM
https://www.readbyqxmd.com/read/28571909/the-presence-of-contaminations-in-sewage-sludge-the-current-situation
#11
Krzysztof Fijalkowski, Agnieszka Rorat, Anna Grobelak, Malgorzata J Kacprzak
Sewage sludge/biosolids are by-wastes of municipal and industrial wastewater treatment. As sources of nutrients (C, N, P) they are widely used in intensive farming where large supplementation of organic matter to maintain fertility and enhance crop yields is needed. However, according to the report of European Commission published in 2010, only 39% of produced sewage sludge is recycled into agriculture in the European Union. This situation occurs mainly due to the fact, that the sewage sludge may contain a dangerous volume of different contaminants...
May 29, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28559930/comparison-of-clinical-and-immunological-findings-in-gnotobiotic-piglets-infected-with-escherichia-coli-o104-h4-outbreak-strain-and-ehec-o157-h7
#12
Bettina Wöchtl, Florian Gunzer, Wilhelm Gerner, Hagen Gasse, Michaela Koch, Zoltán Bagó, Martin Ganter, Herbert Weissenböck, Nora Dinhopl, Sina M Coldewey, Alexandra von Altrock, Karl-Heinz Waldmann, Armin Saalmüller, Kurt Zimmermann, Jörg Steinmann, Jan Kehrmann, Ludger Klein-Hitpass, Jochen Blom, Ralf Ehricht, Ines Engelmann, Isabel Hennig-Pauka
BACKGROUND: Shiga toxin (Stx) producing Escherichia coli (E. coli) (STEC) is the most frequent cause of diarrhoea-positive haemolytic uraemic syndrome (D + HUS) in humans. In 2011, a huge outbreak with an STEC O104:H4 strain in Germany highlighted the limited possibilities for causative treatment of this syndrome. The responsible STEC strain was found to combine Stx production with adherence mechanisms normally found in enteroaggregative E. coli (EAEC). Pathotypes of E. coli evolve and can exhibit different adhesion mechanisms...
2017: Gut Pathogens
https://www.readbyqxmd.com/read/28556680/efficacy-of-gaseous-ozone-application-during-vacuum-cooling-against-escherichia-coli-o157-h7-on-spinach-leaves-as-influenced-by-bacterium-population-size
#13
Mustafa Yesil, David R Kasler, En Huang, Ahmed E Yousef
Foodborne disease outbreaks associated with the consumption of fresh produce pose a threat to public health, decrease consumer confidence in minimally processed foods, and negatively impact the sales of these commodities. The aim of the study was to determine the influence of population size of inoculated pathogen on its inactivation by gaseous ozone treatment during vacuum cooling. Spinach leaves were spot inoculated with Escherichia coli O157:H7 at approximate initial populations of 10(8), 10(7), and 10(5) CFU/g...
May 30, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28542913/reduction-of-escherichia-coli-o157-h7-in-biofilms-using-bacteriophage-bpeco-19
#14
Mohammad Sadekuzzaman, Sungdae Yang, Md Furkanur Rahaman Mizan, Sang-Do Ha
Biofilm formation is a growing concern in the food industry. Escherichia coli O157:H7 is one of the most important foodborne pathogens that can persists in food and food-related environments and subsequently produce biofilms. The efficacy of bacteriophage BPECO 19 was evaluated against three E. coli O157:H7 strains in biofilms. Biofilms of the three E. coli O157:H7 strains were grown on abiotic (stainless steel, rubber, and minimum biofilm eradication concentration [MBEC(TM) ] device) and biotic (lettuce) surfaces at different temperatures...
June 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28525985/investigation-of-optimum-ohmic-heating-conditions-for-inactivation-of-escherichia-coli-o157-h7-salmonella-enterica-serovar-typhimurium-and-listeria-monocytogenes-in-apple-juice
#15
Il-Kyu Park, Jae-Won Ha, Dong-Hyun Kang
BACKGROUND: Control of foodborne pathogens is an important issue for the fruit juice industry and ohmic heating treatment has been considered as one of the promising antimicrobial interventions. However, to date, evaluation of the relationship between inactivation of foodborne pathogens and system performance efficiency based on differing soluble solids content of apple juice during ohmic heating treatment has not been well studied. This study aims to investigate effective voltage gradients of an ohmic heating system and corresponding sugar concentrations (°Brix) of apple juice for inactivating major foodborne pathogens (E...
May 19, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28508923/biomarkers-of-oxidative-damage-in-bacteria-for-the-assessment-of-sanitation-efficacy-in-lettuce-wash-water
#16
Andrea Cossu, Fang Dou, Glenn M Young, Nitin Nitin
In the fresh produce industry, validation of sanitation efficacy is critical to prevent cross-contamination of produce. The current validation approaches are either based on time-consuming plate counting assays or indirect measurements of chemical properties of wash water. In the study, the focus was to identify biomarkers that can provide direct assessment of oxidative damage in bacteria upon exposure to sanitizers in the presence of fresh produce and correlation of these oxidative biomarkers with logarithmic inactivation of bacteria...
July 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28476502/quercetin-suppresses-nlrp3-inflammasome-activation-in-epithelial-cells-triggered-by-escherichia-coli-o157-h7
#17
Yansong Xue, Min Du, Mei-Jun Zhu
Inflammatory responses elicited by LRR and PYD domains-containing protein 3 (NLRP3) inflammasome is induced by a wide variety of stress signals including infectious agents and cellular disorders. E. coli O157:H7 causes serious gastrointestinal diseases that results in severe inflammation and oxidative stress, causing host cell damage. In this study, we found that E. coli O157:H7 infection induced NLRP3 assembly, caspase-1 activation and interleukin (IL)-1β and IL-18 release in Caco-2 cells. Infection also resulted in mitochondrial dysfunction with disrupted mitochondrial potential and mitochondrial complex-I activity, as well as the cytosolic release of cytochrome c and altered mitochondrial respiratory chain...
May 2, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28474971/effect-of-ultrasound-on-some-chemical-and-microbiological-properties-of-sour-cherry-juice-by-response-surface-methodology
#18
Tuğba Türken, Hande S Erge
In this study, it is aimed to determine effect of ultrasonication on some chemical and microbiological properties of sour cherry juice by response surface methodology, since ultrasound is known as an alternative method for thermal food processing. Sour cherry juice was sonicated at varying amplitude levels (50, 75, 100%); moderate temperatures (20, 30, 40 ℃); and treatment times of 2, 6, 10 min at a constant frequency of 20 kHz. Different ultrasonication amplitudes, temperatures, and times had no significant effect on pH,°Bx, and titratable acidity...
January 1, 2017: Food Science and Technology International, Ciencia y Tecnología de Los Alimentos Internacional
https://www.readbyqxmd.com/read/28467186/potential-of-cinnamon-oil-emulsions-as-alternative-washing-solutions-of-carrots
#19
Yue Zhang, Huaiqiong Chen, Faith Critzer, P Michael Davidson, Qixin Zhong
The objective of this study was to evaluate the potential of cinnamon oil emulsions as alternative washing solutions to improve the microbial safety of carrots. Whey protein concentrate (WPC), gum arabic (GA), lecithin, and their combinations were used to prepare cinnamon oil emulsions. The emulsions were characterized for their hydrodynamic diameter (Dh) during 7 days of storage and their antimicrobial activity against cocktails of Salmonella enterica , Escherichia coli O157:H7, and Listeria monocytogenes ...
June 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28452110/antimicrobial-effects-of-quillaja-saponaria-extract-against-escherichia-coli-o157-h7-and-the-emerging-non-o157-shiga-toxin-producing-e-coli
#20
Snigdha Sewlikar, Doris H D'Souza
Natural alternate methods to control the spread of Shiga toxin-producing Escherichia coli (STEC) are important to prevent foodborne outbreaks. Quillaja saponaria aqueous bark extracts (QE), cleared by the U.S. Food and Drug Administration as a natural flavorant, contain bioactive polyphenols, tannins, and tri-terpenoid saponins with anti-inflammatory and antimicrobial activity. The objective of this study was to determine the effects of commercial QE against E. coli O157:H7 and non-O157 strains over 16 h at 37 °C and RT...
May 2017: Journal of Food Science
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