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Escherichia Coli O157 H7 treatment

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https://www.readbyqxmd.com/read/29306732/sequential-effect-of-phages-and-cold-nitrogen-plasma-against-escherichia-coli-o157-h7-biofilms-on-different-vegetables
#1
Haiying Cui, Mei Bai, Lu Yuan, Duraiarasan Surendhiran, Lin Lin
Escherichia coli O157:H7 (E. coli O157:H7) is one of the most common pathogens in fresh vegetables and fruits, and most of the diseases produced by E. coli O157:H7 are associated with biofilms. Cold nitrogen plasma (CNP) is a cold sterilization technique which has no residue. However to completely eliminate the biofilm on the surface of vegetables the processing power and time of CNP have to be enhanced, which will impact on the quality of fruits and vegetables. Thus the sequential treatment of CNP and phage techniques was engineered in this study...
January 3, 2018: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29276663/detection-of-viable-but-non-culturable-escherichia-coli-o157-h7-by-pcr-in-combination-with-propidium-monoazide
#2
Junliang Zhong, Xihong Zhao
The aim of this study was to evaluate the applicability of the conventional PCR detection method combined with propidium monoazide (PMA) treatment for the detection of viable but non-culturable (VBNC) state Escherichia coli O157:H7 in ground beef meatballs. Under low temperature, E. coli O157:H7 cells were induced into the VBNC state in ground beef meatballs at - 20 °C after 152 days. The optimal PMA concentration of 5 µg/mL was obtained in beef meatball samples, which could completely inhibit the DNA amplification on dead cells (106 cells/mL) but with no inhibition on viable cells...
January 2018: 3 Biotech
https://www.readbyqxmd.com/read/29274960/bacterial-diversity-and-composition-of-alfalfa-silage-as-analyzed-by-illumina-miseq-sequencing-effects-of-escherichia-coli-o157-h7-and-silage-additives
#3
I M Ogunade, Y Jiang, A A Pech Cervantes, D H Kim, A S Oliveira, D Vyas, Z G Weinberg, K C Jeong, A T Adesogan
The first objective of this study was to examine effects of adding Escherichia coli O157:H7 with or without chemical or microbial additives on the bacterial diversity and composition of alfalfa silage. The second objective was to examine associations between the relative abundance of known and unknown bacterial species and indices of silage fermentation quality. Alfalfa forage was harvested at 54% dry matter, chopped to a theoretical length of cut of 19 mm, and ensiled in quadruplicate in laboratory silos for 100 d after the following treatments were applied: (1) distilled water (control); (2) 1 × 105 cfu/g of E...
December 20, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/29259594/evaluation-of-sanitizing-methods-for-reducing-microbial-contamination-on-fresh-strawberry-cherry-tomato-and-red-bayberry
#4
Wei Wei, Xu Wang, Zhongwen Xie, Wen Wang, Junfeng Xu, Yuanjing Liu, Haiyan Gao, Yu Zhou
Strawberries, cherry tomatoes, and red bayberries, which are the most popular types of fresh produce in China, are vulnerable to microbial contamination. In this study, different sanitizing methods [treatment with 2% organic acids, 0.02% sodium hypochlorite (SH), 0.1% sodium chlorite (SC), and 0.1% acidified sodium chlorite (ASC)] were applied to fresh strawberry, cherry tomato, and red bayberry, and their abilities to reduce aerobic bacteria, Escherichia coli O157:H7, mold, yeast, and Salmonella Typhimurium were evaluated...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29216383/genetic-susceptibility-to-hemolytic-uremic-syndrome-after-shiga-toxin-producing-escherichia-coli-stec-infection-a-centers-for-disease-control-and-prevention-cdc-foodnet-study
#5
Asha R Kallianpur, Yuki Bradford, Rajal K Mody, Katie N Garman, Nicole Comstock, Sarah L Lathrop, Carol Lyons, Amy Saupe, Katie Wymore, Jeffrey A Canter, Lana M Olson, Amanda Palmer, Timothy F Jones
Background: Post-diarrheal hemolytic-uremic syndrome (D+HUS) following Shiga toxin-producing Escherichia coli (STEC) infection is a serious condition lacking specific treatment. Host immune dysregulation and genetic susceptibility to complement hyperactivation are implicated in non-STEC-related HUS. However, genetic susceptibility to D+HUS remains largely uncharacterized. Methods: Patients with culture-confirmed STEC diarrhea, identified through the CDC FoodNet surveillance system (2007-2012), were serotyped and classified by laboratory and/or clinical criteria as suspected, probable, or confirmed D+HUS, or as controls and genotyped at 200 loci linked to non-diarrheal HUS or similar pathologies...
December 6, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29214002/antibacterial-activity-of-poly-l-arginine-under-different-conditions
#6
Mohaddeseh Sepahi, Razieh Jalal, Mansour Mashreghi
Background and Objectives: Arginine-rich peptides are an important class of antimicrobial peptides (AMPs) that exert their antibacterial activity via a lytic mechanism. Although the antibacterial activity of arginine-rich peptides has been already evaluated, no reports have so far been evaluated the influence of reaction conditions on their antimicrobial potential. The aim of the present study was to investigate the influence of pH, temperature, and glycine on antibacterial activity of poly-l-arginine (PLA) with a molecular weight of 5-15 kDa against Escherichia coli O157:H7 and Staphylococcus aureus...
April 2017: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29196005/inactivation-of-escherichia-coli-o157-h7-and-bacillus-cereus-by-power-ultrasound-during-the-curing-processing-in-brining-liquid-and-beef
#7
Dacheng Kang, Yuhe Jiang, Lujuan Xing, Guanghong Zhou, Wangang Zhang
The objective of this study was to evaluate the effects of ultrasound on the number of Escherichia coli O157:H7 and vegetative cells of Bacillus cereus in brining and beef during the curing processing. The mechanisms of the inactivation for the pathogens were preliminarily explored. The ultrasound intensity (2.39, 6.23, 11.32 and 20.96Wcm-2) and sonication time (30, 60, 90 and 120min) at 10°C were assessed in brine and meat. Plate count data indicated that 20.96Wcm-2 for 120min was the optimal treatment for bacterial reductions...
December 2017: Food Research International
https://www.readbyqxmd.com/read/29179098/the-antibacterial-activity-of-clove-oil-chitosan-nanoparticles-embedded-gelatin-nanofibers-against-escherichia-coli-o157-h7-biofilms-on-cucumber
#8
Haiying Cui, Mei Bai, Marwan M A Rashed, Lin Lin
This study aims to evaluate the antibacterial activity of clove oil-loaded chitosan nanoparticles (CO@CNPs) and gelatin electrospun nanofibers against Escherichia coli O157:H7 (E. coli O157:H7) biofilms on cucumbers. The optimal CO@CNPs were prepared when the initial concentration of clove oil (CO) was 2.5mg/mL according to the ionic crosslinking method. CO@CNPs showed high antibacterial activity against E. coli O157:H7 biofilms. After 8h treatment, almost 99.98% reduction in E. coli O157:H7 population was achieved when CO@CNPs were applied at 30% (w/v)...
November 24, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29130764/efficacy-of-a-blend-of-sulfuric-acid-and-sodium-sulfate-against-shiga-toxin-producing-escherichia-coli-salmonella-and-nonpathogenic-escherichia-coli-biotype-i-on-inoculated-prerigor-beef-surface-tissue
#9
Britteny R Scott-Bullard, Ifigenia Geornaras, Robert J Delmore, Dale R Woerner, James O Reagan, J Bred Morgan, Keith E Belk
A study was conducted to investigate the efficacy of a sulfuric acid-sodium sulfate blend (SSS) against Escherichia coli O157:H7, non-O157 Shiga toxin-producing E. coli (STEC), Salmonella, and nonpathogenic E. coli biotype I on prerigor beef surface tissue. The suitability of using the nonpathogenic E. coli as a surrogate for in-plant validation studies was also determined by comparing the data obtained for the nonpathogenic inoculum with those for the pathogenic inocula. Prerigor beef tissue samples (10 by 10 cm) were inoculated (ca...
December 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/29115532/application-of-f0f1%C3%A2-atpase-immuno%C3%A2-biosensors-for-detecting-escherichia%C3%A2-coli-o157-h7
#10
Ling Wei, Bao-Ming Li, Cheng-Bin Wang, Zi-Jia Kang, Jie Sun, Hui-Juan Wu, Yong-Zhi Lun
Escherichia coli (E. coli) O157:H7 is an important food‑borne pathogen with a low infective threshold and high resistance to treatment. There are currently a number of detection methods available, however, the majority are time‑consuming, complex and expensive, thus it is hard for these methods to be applied in routine detection. Therefore, there is urgent requirement to develop more sensitive, rapid and specific detective techniques. In the present study, an immuno‑biosensor based on the interference of load to the F0F1‑ATPase rotation, indicated by the fluorescence fluctuation, was constructed to detect O157:H7...
November 7, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29111062/plasma-treated-poly-ethylene-oxide-nanofibers-containing-tea-tree-oil-beta-cyclodextrin-inclusion-complex-for-antibacterial-packaging
#11
Haiying Cui, Mei Bai, Lin Lin
This work describes the effect of cold nitrogen plasma to enhance the antibacterial activity of poly(ethylene oxide) (PEO) nanofibers containing antibacterial agent. Beta-cyclodextrin (β-CD) and tea tree oil (TTO) were used as a host-guest to form water-soluble inclusion complex. The encapsulation efficiency of TTO in inclusion complex could reach 73.23% at 60°C. As antibacterial agent, the inclusion complex was encapsulated into PEO matrix by electrospun. After plasma treatment, the release efficiency of antibacterial agent from PEO nanofibers was improved...
January 1, 2018: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29072326/antimicrobial-activity-of-suspensions-and-nanoemulsions-of-citral-in-combination-with-heat-or-pulsed-electric-fields
#12
E Pagán, D Berdejo, L Espina, D García-Gonzalo, R Pagán
The application of essential oils in form of nanoemulsions has been proposed as a method to improve their solubility in aqueous solutions, and hence their antimicrobial activity. The objective of this study was to evaluate the antimicrobial activity of citral, applied directly or in combined treatments with heat or pulsed electric fields (PEF), as a function of the inoculation procedure assayed: (i) a simple, vigorous shaking method by vortex agitation (suspension of citral; s-citral) or (ii) the previous preparation of nanoemulsions by the emulsion phase inversion (EPI) method (nanoemulsion of citral; n-citral)...
October 26, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29051644/chemical-compositions-antioxidant-and-antimicrobial-properties-of-ziziphora-clinopodioides-lam-essential-oils-collected-from-different-parts-of-iran
#13
Yasser Shahbazi
The aims of the present study were to investigate chemical compositions, antioxidant and antimicrobial properties of Ziziphora clinopodioides essential oils (ZEOs) collected from four provinces in western Iran (Ilam, Lorestan, Kermanshah and Kurdestan). Carvacrol was the most abundant constituent in the flower, stem and leaf oil samples of Ilam, Lorestan and Kermanshah regions by 73.12-74.29%, 66.47-66.89% and 65.11-65.32%, respectively. The most abundant components in Kurdestan sample were thymol (55.32-55...
October 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/29044827/identifying-the-mechanism-of-escherichia-coli-o157-h7-survival-by-the-addition-of-salt-in-the-treatment-with-organic-acids
#14
Young-Min Bae, Jae-Hyun Yoon, Ji-Yeon Kim, Sun-Young Lee
AIM: In this study, the effects of addition of salt to treatment with acids (one of several organic acids and salt in various solutions including rich or minimal broth, buffer, or distilled water) on the reduction of Escherichia coli O157:H7 were investigated. The protein expression profiles corresponding to acid stress (acetic acid) with or without salt addition were studied using a comparative proteomic analysis of E. coli O157:H7. METHODS AND RESULTS: When acetic, lactic, or propionic acid was combined with 3% NaCl, mutually antagonistic effects of acid and salt on viability of E...
October 17, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29035438/high-throughput-screening-of-escherichia-coli-o157-h7-pathogenic-genes-via-pathway-enrichment-and-operon-analysis
#15
Guangxin Yuan, Jia Fu, Liping An, Yan Zhuang, Yu Wang, Yunfeng Song, Zijing Qiao, Xiaolong Wang, Yufei Tian, Xiao Han, Peige Du, Guangyu Xu
BACKGROUND: About thirty thousand people globally die every day from infectious diarrhea, mostly caused by pathogenic Escherichia coli (E. coli) O157:H7. METHODS: In order to search for clinical diagnostic biomarkers and novel drug targets for infectious diarrhea, we used a bibliometric method to collect pathogenic genes of E. coli O157:H7 and performed a functional analysis of the important pathogenic genes by pathway enrichment and operon analysis. RESULTS: We found 364 pathogenic genes which may be involved in infection with E...
October 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28975041/escherichia-coli-o157-h7-suppresses-host-autophagy-and-promotes-epithelial-adhesion-via-tir-mediated-and-camp-independent-activation-of-protein-kinase-a
#16
Yansong Xue, Min Du, Haiqing Sheng, Carolyn J Hovde, Mei-Jun Zhu
Autophagy is a pivotal innate immune response that not only degrades cytosolic components, but also serves as one of the critical antimicrobial mechanisms eliminating intracellular pathogens. However, its role in host defense against extracellular pathogens is largely unknown. Here we showed that E. coli O157:H7 altered autophagy to evade host defense and facilitate adhesion. Enhancing host cell autophagy with tumor necrosis factor (TNF), host starvation or rapamycin reduced the adherence of E. coli O157:H7 to HT-29 cells...
2017: Cell Death Discovery
https://www.readbyqxmd.com/read/28884271/the-effect-of-anaerobic-digestion-and-storage-on-indicator-microorganisms-in-swine-and-dairy-manure
#17
Annamaria Costa, Claudia Gusmara, Davide Gardoni, Mauro Zaninelli, Fulvia Tambone, Vittorio Sala, Marcella Guarino
The aim of this experimental study was to evaluate the influence of anaerobic digestion and storage on indicator microorganisms in swine and dairy excreta. Samples were collected every 90 days for 15 months at eight farms, four pig, and four dairy farms, four of them having a biogas plant. Moreover, to evaluate storage effects on samples, 20 l of manure and slurry taken at each farm (digested manure only in farms with a biogas plant) were stored in a controlled climatic chamber at 18 °C, for 6 months...
November 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28865862/rapid-and-simultaneous-quantification-of-viable-escherichia-coli-o157-h7-and-salmonella-spp-in-milk-through-multiplex-real-time-pcr
#18
Baoqing Zhou, Taobo Liang, Zhongxu Zhan, Rui Liu, Fan Li, Hengyi Xu
Escherichia coli O157:H7 and Salmonella spp. in milk are 2 common pathogens that cause foodborne diseases. An accurate, rapid, specific method has been developed for the simultaneous detection of viable E. coli O157:H7 and Salmonella spp. in milk. Two specific genes, namely, fliC from E. coli O157:H7 and invA from Salmonella spp., were selected to design primers and probes. A combined treatment containing sodium deoxycholate (SDO) and propidium monoazide (PMA) was applied to detect viable E. coli O157:H7 and Salmonella spp...
November 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28852487/rare-genetic-variants-in-shiga-toxin-associated-haemolytic-uraemic-syndrome-genetic-analysis-prior-to-transplantation-is-essential
#19
Frances Dowen, Katrina Wood, Alison L Brown, Jennifer Palfrey, David Kavanagh, Vicky Brocklebank
We present a case of haemolytic uraemic syndrome (HUS) in a 16-year-old female with serological evidence of acute Escherichia coli O157:H7 infection. She progressed to established renal failure and received a deceased donor kidney transplant. Shiga toxin-associated HUS (STEC-HUS) does not recur following renal transplantation, but unexpectedly this patient did experience rapid and severe HUS recurrence. She responded to treatment with the terminal complement inhibitor eculizumab and subsequent genetic analysis revealed a rare variant in a complement gene...
August 2017: Clinical Kidney Journal
https://www.readbyqxmd.com/read/28843125/combination-of-essential-oil-compounds-and-phenolic-acids-against-escherichia-coli-o157-h7-in-vitro-and-in-dry-fermented-sausage-production
#20
Natan V B Meira, Richard A Holley, Keliani Bordin, Renata E F de Macedo, Fernando B Luciano
Escherichia coli O157:H7 is a foodborne pathogen that causes hemorrhagic colitis and hemolytic uremic syndrome. The low dose of infection and severity of the disease represent a concern to public health. Natural compounds have been widely applied as food additives to replace synthetic preservatives. The aim of this study was to determine the efficiency of essential oil compounds (EOCs) in combination with phenolic acids (PA) in vitro and in dry-fermented sausage production. Minimum Inhibitory Concentration (MIC) and Fractional Inhibitory Concentration index (FICindex) were determined for a 5-strain mixture of E...
August 16, 2017: International Journal of Food Microbiology
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