keyword
https://read.qxmd.com/read/34116244/development-and-validation-of-signature-sequence-based-pcr-for-improved-molecular-diagnosis-of-tuberculosis
#21
JOURNAL ARTICLE
Neha Quadir, Syed Asad Rahman, Javeed Ahmad, Ayan Kumar Das, Naresh Arora, Javaid Ahmad Sheikh, Neeraj Kumar Gupta, Prem Kapur, Mridu Dudeja, Seyed E Hasnain, Nasreen Z Ehtesham
Reliable, fast, and affordable diagnosis for TB remains a challenge to reduce disease incidence in resource-poor countries. Tests based on nucleotide sequences that are 'signature' to M. tuberculosis (M. tb) have the potential to make a positive impact on case detection rates, which can eventually help control TB. Using extensive comparative bioinformatics approach, we mined the genome for M. tb-specific genes and identified four genes so-called "Signature sequence (SS)". With <25% homology with other known genes/proteins of mycobacterial/non-mycobacterial origin in various databases, these SS-genes are ideal targets for species-specific identification...
June 8, 2021: Journal of Molecular Diagnostics: JMD
https://read.qxmd.com/read/33819222/correlation-of-c-reactive-protein-levels-gene-polymorphism-and-platelets-count-in-dengue-infection
#22
JOURNAL ARTICLE
Sana Shamshad, Sara Khan, Ghazala Kaukab Raja, Muhammad Sheeraz Ahmad, Muhammad Javaid Asad, Tayyaba Zainab
OBJECTIVE: To determine the correlation of polymorphism in C-reactive protein gene with variation in serum levels in dengue patients. METHODS: The cross-sectional study was conducted at Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan, from October 2017 to October 2018, and comprised blood samples from dengue patients which were used to measure the serum levels of C-reactive protein. Deoxyribonucleic acid extraction followed by tetra amplification-refractory mutation system polymerase chain reaction was used to analyse the genotype variation T>G for single nucleotide polymorphism rs199953854 using allele-specific primers...
February 2021: JPMA. the Journal of the Pakistan Medical Association
https://read.qxmd.com/read/33726649/in-silico-validation-fabrication-and-evaluation-of-nano-liposomes-of-bistorta-amplexicaulis-extract-for-improved-anticancer-activity-against-hepatoma-cell-line-hepg2
#23
JOURNAL ARTICLE
Salma Batool, M Javaid Asad, Muhammad Arshad, Warda Ahmed, Muhammad Farhan Sohail, Sumra Wajid Abbasi, Sajjad Ahmad, Rahman Shah Zaib Saleem, Muhammad Sheeraz Ahmed
BACKGROUND: Bistorta amplexicaulis of the genus Polygonum (Polygonaceae) has been reported for its antioxidant and anticancer activities. However, the low cellular uptake of the compounds in its extract limit their therapeutic application. OBJECTIVES: The present study was aimed at developing a nanoliposomal carrier system for B. amplexicaulis extracts for its improved cellular uptake thus resulting in enhanced anticancer activity. METHODS: Ultra Pressure Liquid Chromatography (UPLC) was used to identify major compounds in the plant extract...
March 16, 2021: Current Drug Delivery
https://read.qxmd.com/read/33163415/-mycobacterium-smegmatis-bacteria-expressing-mycobacterium-tuberculosis-specific-rv1954a-induce-macrophage-activation-and-modulate-the-immune-response
#24
JOURNAL ARTICLE
Simran Kaur Arora, Nilofer Naqvi, Anwar Alam, Javeed Ahmad, Basma Saud Alsati, Javaid Ahmad Sheikh, Prabin Kumar, Dipendra Kumar Mitra, Syed Asad Rahman, Seyed Ehtesham Hasnain, Nasreen Zafar Ehtesham
Mycobacterium tuberculosis ( M. tb ), the intracellular pathogen causing tuberculosis, has developed mechanisms that endow infectivity and allow it to modulate host immune response for its survival. Genomic and proteomic analyses of non-pathogenic and pathogenic mycobacteria showed presence of genes and proteins that are specific to M. tb . In silico studies predicted that M.tb Rv1954A is a hypothetical secretory protein that exhibits intrinsically disordered regions and possess B cell/T cell epitopes. Treatment of macrophages with Rv1954A led to TLR4-mediated activation with concomitant increase in secretion of pro-inflammatory cytokines, IL-12 and TNF-α...
2020: Frontiers in Cellular and Infection Microbiology
https://read.qxmd.com/read/32793184/immunodominant-mycobacterium-tuberculosis-protein-rv1507a-elicits-th1-response-and-modulates-host-macrophage-effector-functions
#25
JOURNAL ARTICLE
Simran Kaur Arora, Anwar Alam, Nilofer Naqvi, Javeed Ahmad, Javaid Ahmad Sheikh, Syed Asad Rahman, Seyed Ehtesham Hasnain, Nasreen Zafar Ehtesham
Mycobacterium tuberculosis ( M. tb ) persists as latent infection in nearly a quarter of the global population and remains the leading cause of death among infectious diseases. While BCG is the only vaccine for TB, its inability to provide complete protection makes it imperative to engineer BCG such that it expresses immunodominant antigens that can enhance its protective potential. In-silico comparative genomic analysis of Mycobacterium species identified M. tb Rv1507A as a "signature protein" found exclusively in M...
2020: Frontiers in Immunology
https://read.qxmd.com/read/32474554/mapping-the-genomic-landscape-diversity-of-covid-19-based-on-3950-clinical-isolates-of-sars-cov-2-likely-origin-transmission-dynamics-of-isolates-sequenced-in-india
#26
JOURNAL ARTICLE
Hina Singh, Jasdeep Singh, Mohd Khubaib, Salma Jamal, Javaid Ahmed Sheikh, Sunil Kohli, Seyed Ehtesham Hasnain, Syed Asad Rahman
No abstract text is available yet for this article.
May 30, 2020: Indian Journal of Medical Research
https://read.qxmd.com/read/32335334/emerging-genetic-diversity-among-clinical-isolates-of-sars-cov-2-lessons-for-today
#27
JOURNAL ARTICLE
Javaid Ahmad Sheikh, Jasdeep Singh, Hina Singh, Salma Jamal, Mohd Khubaib, Sunil Kohli, Ulrich Dobrindt, Syed Asad Rahman, Nasreen Zafar Ehtesham, Seyed Ehtesham Hasnain
Considering the current pandemic of COVID-19, it is imperative to gauge the role of molecular divergence in SARS-CoV-2 with time, due to clinical and epidemiological concerns. Our analyses involving molecular phylogenetics is a step toward understanding the transmission clusters that can be correlated to pathophysiology of the disease to gain insight into virulence mechanism. As the infections are increasing rapidly, more divergence is expected followed possibly by viral adaptation. We could identify mutational hotspots which appear to be major drivers of diversity among strains, with RBD of spike protein emerging as the key region involved in interaction with ACE2 and consequently a major determinant of infection outcome...
October 2020: Infection, Genetics and Evolution
https://read.qxmd.com/read/31576931/apparent-nutrient-digestibility-of-carbohydrate-corn-in-cirrhinus-mrigala-under-the-influence-of-chromium-chloride-hexahydrate
#28
JOURNAL ARTICLE
Farkhanda Asad, Samina Qamer, Asma Ashraf, Azhar Rafique, Zunaira Shaheen, Ayesha Nisar, Sidra Javaid
A feeding trial was carried out to assess the effect of dietary chromium supplementation on apparent nutrient digestibility coefficient (%) of gelatinized and non-gelatinized corn in Cirrhinus mrigala fingerlings for 90 days. Using various levels of chromium chloride hexahydrate six test diets designated as T1 (G/0.0 Cr2Cl3.6H2O mg Kg-1), T2 (NG/0.0 Cr2Cl3.6H2O mg Kg-1), T3 (G/0.2 Cr2Cl3.6H2O mg Kg-1), T4 (NG/0.2 Cr2Cl3.6H2O mg Kg-1), T5 (G/0.4 Cr2Cl3.6H2O mg Kg-1) and T6 (NG/0.4 Cr2Cl3.6H2O mg Kg-1) were prepared...
September 26, 2019: Brazilian Journal of Biology
https://read.qxmd.com/read/31032159/retraction-neurogenic-stunned-myocardium-a-literature-review
#29
JOURNAL ARTICLE
Asad Ali, Malik Qistas Ahmad, Muhammad Bilal Malik, Zara Z Alvi, Waleed Iftikhar, Deepak Kumar, Usama Nasir, Nouman Safdar Ali, Zohaib Sayyed, Risham Javaid, Neha Waqas, Shahzad Ahmed Sami, Abbas M Cheema
[This retracts the article DOI: 10.7759/cureus.3129.].
April 16, 2019: Curēus
https://read.qxmd.com/read/30974139/crystallization-thermal-stability-barrier-property-and-aging-resistance-application-of-multi-functionalized-graphene-oxide-poly-lactide-starch-nanocomposites
#30
JOURNAL ARTICLE
Pengkai Wang, ShangWen Tang, Feng Sheng, Jie Cai, Peng Fei, Asad Nawaz, Noman Walayat, Allah Bakhsh Javaid, Hanguo Xiong
Poly(lactide)-starch matrix, blended with multi-functionalized graphene oxide, was synthesized by solution casting in this study. To improve its interface compatibility, the graphene oxide (GO) was grafted with maleic anhydride and subsequently modified by dodecyl amine. The chemical structure and morphology of functionalized GO (f-GO) were determined by Fourier transform infrared (FTIR) spectroscopy, X-ray photoelectron spectroscopy (XPS), and transmission electron microscopy (TEM). The crystallization property, surface wettability, morphology, thermal stability, and dynamic mechanical and aging resistance properties of the nanocomposite were determined...
April 8, 2019: International Journal of Biological Macromolecules
https://read.qxmd.com/read/30372488/correction-application-of-the-doylestown-algorithm-for-the-early-detection-of-hepatocellular-carcinoma
#31
Anand S Mehta, Daryl T-Y Lau, Mengjun Wang, Aysha Aslam, Bilal Nasir, Asad Javaid, Mugilan Poongkunran, Timothy M Block
[This corrects the article DOI: 10.1371/journal.pone.0203149.].
2018: PloS One
https://read.qxmd.com/read/30345188/neurogenic-stunned-myocardium-a-literature-review
#32
REVIEW
Asad Ali, Malik Qistas Ahmad, Muhammad Bilal Malik, Zara Z Alvi, Waleed Iftikhar, Deepak Kumar, Usama Nasir, Nouman Safdar Ali, Zohaib Sayyed, Risham Javaid, Neha Waqas, Shahzad Ahmed Sami, Abbas M Cheema
Neurogenic stunned myocardium (NSM) is defined as the occurrence of cardiac abnormalities due to neurological events such as stroke, seizures, etc. These events lead to dysfunction of the autonomic nervous system (ANS) and ultimately cause injury to the myocardium. The clinical features seen in NSM include elevated troponin level, left ventricular dysfunction, and changes on the electrocardiogram (ECG). However, these features are also seen in Takotsubo cardiomyopathy as well as in an acute coronary syndrome (ACS)...
August 10, 2018: Curēus
https://read.qxmd.com/read/30186709/spontaneous-left-anterior-descending-artery-dissection-in-a-middle-aged-woman-with-vitamin-b12-deficiency-treated-with-coronary-artery-bypass-grafting
#33
JOURNAL ARTICLE
Quratulain Fatima Masood, Ali Asad, Syed Maaz Tariq, Saad Javaid, Muhammad H Khalil, Fiaz Hussain
Spontaneous coronary artery dissection (SCAD) is one of the rarest causes of acute coronary syndromes, which include myocardial infarction (MI), stable and unstable angina, cardiogenic shock, and sudden death. The course of the disease, its etiology, prevalence, prognosis, and treatment remain ill-defined. Adding to the complexity is the fact that patients may lack typical risk factors for coronary heart disease. Herein, we report a case of a 42-year-old woman with vitamin B12 deficiency, who presented with chest pain; electrocardiography (ECG) findings were consistent with the acute anterior wall MI...
July 1, 2018: Curēus
https://read.qxmd.com/read/30169533/application-of-the-doylestown-algorithm-for-the-early-detection-of-hepatocellular-carcinoma
#34
JOURNAL ARTICLE
Anand S Mehta, Daryl T-Y Lau, Mengjun Wang, Aysha Islam, Bilal Nasir, Asad Javaid, Mugilan Poongkunran, Timothy M Block
BACKGROUND: We previously developed a logistic regression algorithm that uses AFP, age, gender, ALK and ALT levels to improve the detection of hepatocellular carcinoma (HCC). In 3,158 patients from 5 independent sites, this algorithm, referred to as the "Doylestown" algorithm, increased the AUROC of AFP 4% to 12% and had equal benefit regardless of tumor size or the etiology of liver disease. AIMS: Analysis of the Doylestown algorithm using samples from individuals taken before their diagnosis of HCC...
2018: PloS One
https://read.qxmd.com/read/30149631/iot-operating-system-based-fuzzy-inference-system-for-home-energy-management-system-in-smart-buildings
#35
JOURNAL ARTICLE
Sohail Iqbal, Safdar Abbas Khan, Asad Waqar Malik, Iftikhar Ahmad, Nadeem Javaid
Energy consumption in the residential sector is 25% of all the sectors. The advent of smart appliances and intelligent sensors have increased the realization of home energy management systems. Acquiring balance between energy consumption and user comfort is in the spotlight when the performance of the smart home is evaluated. Appliances of heating, ventilation and air conditioning constitute up to 64% of energy consumption in residential buildings. A number of research works have shown that fuzzy logic system integrated with other techniques is used with the main objective of energy consumption minimization...
August 25, 2018: Sensors
https://read.qxmd.com/read/29510254/the-functionality-of-prebiotics-as-immunostimulant-evidences-from-trials-on-terrestrial-and-aquatic-animals
#36
REVIEW
Asad Nawaz, Allah Bakhsh Javaid, Sana Irshad, Seyed Hossein Hoseinifar, Hanguo Xiong
The gut immune system is, the main option for maintaining host's health, affected by numerous factors comprising dietary constituents and commensal bacteria. These dietary components that affect the intestinal immunity and considered as an alternative of antibiotics are called immunosaccharides. Fructooligosaccharide (FOS), Galactooligosaccharide (GOS), inulin, dietary carbohydrates, and xylooligosaccharide (XOS) are among the most studied prebiotics in human as well as in aquaculture. Although prebiotics and probiotics have revealed potential as treatment for numerous illnesses in both human and fish, a comprehensive understanding of the molecular mechanism behind direct and indirect effect on the intestinal immune response will help more and perhaps extra effective therapy intended for ailments...
May 2018: Fish & Shellfish Immunology
https://read.qxmd.com/read/29142550/clinical-outcome-of-23g-trans-conjunctival-pars-plana-vitrectomy-a-prospective-comparison-of-phaco-vitrectomy-with-only-vitrectomy-in-phakic-eyes
#37
JOURNAL ARTICLE
Haroon Tayyab, Asad Aslam Khan, Rana Muhammad Mohsin Javaid
Objective: To evaluate the effectiveness and safety profile of combined phacoemulsification with 23G pars plana vitrectomy when compared to pars plana vitrectomy alone in phakic patients. Methods: This study was performed at Al-Ehsan Eye Hospital (tertiary care eye hospital in Lahore, Pakistan) from January 2016 to August 2016. A total of 40 eyes in two equal groups of 20 eyes each, were enrolled in this prospective study. Group-A underwent combined phaco-vitrectomy, whereas Group-B underwent vitrectomy only for various vitreoretinal pathologies...
September 2017: Pakistan Journal of Medical Sciences Quarterly
https://read.qxmd.com/read/28924289/knowledge-attitude-and-practices-kap-of-the-families-of-b-thalassaemia-children-in-thalassaemia-centers-of-rawalpindi-and-islamabad-pakistan
#38
JOURNAL ARTICLE
Aamir Shahzad, Nazia Rafiq, Ikram Ullah, Muhammad Javaid Asad, Muhammad Sheeraz Ahmad, Usman Waheed
The present study was designed to assess the Knowledge, Attitude and Practices (KAP) of the parents of b-thalassaemia children (410) selected from public (73.2%) and private (26.8%) thalassaemia centers of Rawalpindi-Islamabad. Qualitative and quantitative approaches were used to collect the data, which was analyzed by using SPSS. Majority of the respondents (70%) were rural young parents with no knowledge of thalassaemia before marriage. However, now 81.2% were aware about this. Majority of the respondents (89%) had the knowledge about premarital screening, 86...
September 2017: JPMA. the Journal of the Pakistan Medical Association
https://read.qxmd.com/read/28873034/the-absence-of-hcv-rna-and-ns5a-protein-in-peripheral-blood-mononuclear-cells-is-a-prognostic-tool-for-sustained-virological-response
#39
JOURNAL ARTICLE
Azhar Mehmood, Muhammad Javaid Asad, Muhammad Ovais, Nasib Zaman, Hafsa Aziz, Javaid Irfan, Irshad Ahmad, Abida Raza
Hepatitis C Virus (HCV) infection is a major health concern worldwide. The presence of both HCV viral RNA and NS5A proteins in peripheral blood mononuclear cells (PBMCs) indicate the efficacy of the treatment during sustained virological response (SVR) and end of treatment response (ETR). The main objective of this study was to detect the absence or presence of HCV RNA and NS5A proteins in PBMCs. Blood samples were taken from selected patients (Islamabad, Pakistan) before treatment, at ETR, and during SVR. Two hundred HCV responders to pegylated IFN-α-2a plus ribavirin were selected...
October 2017: Viral Immunology
https://read.qxmd.com/read/28375125/hypoglycemic-and-hypocholesterolemic-activity-of-leave-of-few-medicinal-plants-against-steptozotocin-induced-hyperglycemia
#40
JOURNAL ARTICLE
Asma Ahmed Asma Ahmed, Muhammad Gulfraz, Muhammad Javaid Asad, Rehmatullah Qureshi, Shabnam Bibi, Syed Imam Shah
Diabetes mellitus (DM) is universal of the hormonal problem and Type II diabetes is foremost obstacle. Accessible management in medicine has numerous contrary paraphernalia. Medical flora shows an essential part in managing diabetes specifically in unindustrialized nations. The present study was done on leaves of Rhazya stricta Decane, Adhatoda zeylanica, Berberis lycium Royle and Olea furrrignea, whose methanolic extracts were used to check their hypoglycemic and hypolipidemic activity by using glucometer and kit method respectively in blood of male and female albino mice Balb C...
November 2016: Pakistan Journal of Pharmaceutical Sciences
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