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https://www.readbyqxmd.com/read/28728394/characterization-of-porcine-cytokine-inducible-sh2-containing-protein-gene-and-its-association-with-piglet-diarrhea-traits
#1
Buyue Niu, Dongchun Guo, Zhiran Liu, Xiaofei Han, Xibiao Wang
Objective: The Cytokine inducible SH2-containing protein (CISH), which might play a role in porcine intestine immune responses, was one of the promising candidate genes for piglet anti-disease traits. An experiment was conducted to characterize the porcine CISH (pCISH) gene and to evaluate its genetic effects on pig anti-disease breeding. Methods: Both RT-PCR and PCR were performed to obtain the sequence of pCISH gene. A pEGFP- C1- CISH vector was constructed and transfected into PK-15 cells to analysis the distribution of pCISH...
May 14, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28728299/-the-value-of-b7-h4-and-carcinoembryonic-antigen-in-diagnosing-the-benign-and-malignant-pleural-effusion
#2
F Wei, Y Wei, L F Li, G L Li, G J Wang
Objective: To evaluate the value of combined detection of negative costimulatory molecule B7-H4 and carcinoembryonic antigen (CEA) in diagnosing malignant and benign pleural effusion. Methods: Ninety-seven pleural effusion specimen were collected, 55 of which were diagnosed as malignant pleural effusion and 42 were benign pleural effusion. Enzyme-linked immunosorbent assay(ELISA) was used to examine the concentration of B7-H4 and CEA in pleural effusion. Electro-chemiluminescence immunoassay was used to detect the CEA level in pleural effusion...
July 23, 2017: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
https://www.readbyqxmd.com/read/28728295/-effect-of-21-gene-recurrence-score-on-chemotherapy-decisions-for-patients-with-estrogen-receptor-positive-epidermal-growth-factor-receptor-2-negative-and-lymph-node-negative-early-stage-breast-cancer
#3
Y Mao, X S Chen, Y Liang, J Y Wu, O Huang, Y Zong, Q Fang, J R He, L Zhu, W G Chen, Y F Li, L Lin, X C Fei, K W Shen
Objective: To investigate the effect of 21-gene recurrence score on adjuvant chemotherapy decisions for patients with estrogen receptor (ER)-positive, epidermal growth factor receptor 2 (HER-2)-negative and lymph node (LN)-negative early stage-breast cancer. Methods: One hundred and forty-eight patients with ER+ , HER-2- and LN- early stage breast cancer were recruited in the Ruijin hospital, Shanghai Jiao Tong University School of Medicine. The 21-gene recurrence score (RS)assay was performed and systemic therapeutic decisions were made before and after knowing the RS results under multidisciplinary discussion...
July 23, 2017: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
https://www.readbyqxmd.com/read/28728294/-depletion-of-gp73-inhibits-invasion-and-metastasis-of-hepatocellular-carcinoma-cells
#4
L P Liu, J K Chen, Y M Liu, D H Zhang, J Zhang, X L Yang
Objective: To explore the in vitro and in vivo effect of GP73 on the proliferation, invasion and metastasis in hepatocellular carcinoma. Methods: GP73 gene was knocked out using CRISPR/Cas9 gene editing system in H22 and HepG2 cells, and stable knock out strains were constructed. The knockout efficiency was measured by western blot. Colony formation assay was used to detect the effect of GP73 on long-term survival ability. Cells were then highly synchronized in G(1) phase upon treatment with cell synchronization reagents (mimosine), and the percentage of cells in G(2)/M phase at different time points was detected by flow cytometry...
July 23, 2017: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
https://www.readbyqxmd.com/read/28728293/-curcumin-induces-apoptosis-and-protective-autophagy-in-human-gastric-cancer-cells-with-different-degree-of-differentiation
#5
W Li, Y Zhou, J Yang, H H Zhang, S L Zhao, T Zhang, J Huo, P Zheng
Objective: To investigate the effect of curcumin on the apoptosis and autophagy of human gastric cancer cells with different degree of differentiation. Methods: Gastric cancer cell lines BGC-823 and MKN-28 were treated with curcumin at different concentrations. The effect of curcumin on cell proliferation was measured by MTT assay. Apoptosis was assessed by flow cytometry. Autophagy status was analyzed by acridine orange staining. The expression levels of apoptotic and autophagy-related proteins were detected by Western blot...
July 23, 2017: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
https://www.readbyqxmd.com/read/28728239/-effect-of-hydrogen-rich-saline-on-the-cd4-cd25-foxp3-treg-cells-of-allergic-rhinitis-guinea-pigs-model
#6
F F Xu, S Q Yu, C L Zhao, N Che, L Jin, Q L Wang, R M Ge
Objective: To explore the effect of hydrogen-rich saline on the CD4(+) CD25(+) Foxp3(+) Treg cells in a guinea pig model of allergic rhinitis (AR) and investigate the underling anti-inflammatory mechanism. Methods: Using random number table, eighteen guinea pigs were divided into three groups (control group/AR group/HRS group, n=6 of each group). AR guinea pig model was built with ovalbumin and aluminum. The guinea pigs were injected with hydrogen-rich saline (HRS group) for ten days after sensitation. And control group was injected with equal normal saline at the same time...
July 7, 2017: Zhonghua Er Bi Yan Hou Tou Jing Wai Ke za Zhi, Chinese Journal of Otorhinolaryngology Head and Neck Surgery
https://www.readbyqxmd.com/read/28728156/predicting-the-risk-to-develop-preeclampsia-in-the-first-trimester-combining-promoter-variant-98a-c-of-lgals13-placental-protein-13-black-ethnicity-previous-preeclampsia-obesity-and-maternal-age
#7
Liora Madar-Shapiro, Ido Karady, Alla Trahtenherts, Argryo Syngelaki, Ranjit Akolekar, Liona Poon, Ruth Cohen, Adi Sharabi-Nov, Berthold Huppertz, Marei Sammar, Kata Juhasz, Nandor Gabor Than, Zoltan Papp, Roberto Romero, Kypros H Nicolaides, Hamutal Meiri
BACKGROUND: LGALS13 (placental protein 13 [PP13]) promoter DNA polymorphisms was evaluated in predicting preeclampsia (PE), given PP13's effects on hypotension, angiogenesis, and immune tolerance. METHODS: First-trimester plasma samples (49 term and 18 intermediate) of PE cases matched with 196 controls were collected from King's College Hospital, London, repository. Cell-free DNA was extracted and the LGALS13 exons were sequenced after PCR amplification. Expression of LGALS13 promoter reporter constructs was determined in BeWo trophoblast-like cells with luciferase assays...
July 21, 2017: Fetal Diagnosis and Therapy
https://www.readbyqxmd.com/read/28728138/first-trimester-urinary-bisphenol-a-concentration-in-relation-to-anogenital-distance-an-androgen-sensitive-measure-of-reproductive-development-in-infant-girls
#8
Emily S Barrett, Sheela Sathyanarayana, Omar Mbowe, Sally W Thurston, J Bruce Redmon, Ruby H N Nguyen, Shanna H Swan
INTRODUCTION: Evidence from animal models suggests that prenatal exposure to bisphenol A (BPA), a ubiquitous endocrine-disrupting chemical, is associated with adverse reproductive outcomes in females. Exposure during early gestation, a critical period for reproductive development, is of particular concern. Anogenital distance (AGD) is a sensitive biomarker of the fetal hormonal milieu and a measure of reproductive toxicity in animal models. In some studies, the daughters of BPA-exposed dams have shorter AGD than controls...
July 11, 2017: Environmental Health Perspectives
https://www.readbyqxmd.com/read/28728123/analysis-of-sugarcane-herbicides-in-marine-turtle-nesting-areas-and-assessment-of-risk-using-in%C3%A2-vitro-toxicity-assays
#9
Hannah L Allan, Jason P van de Merwe, Kimberly A Finlayson, Jake W O'Brien, Jochen F Mueller, Frederic D L Leusch
Agricultural processes are associated with many different herbicides that can contaminate surrounding environments. In Queensland, Australia, herbicides applied to agricultural crops may pose a threat to valuable coastal habitats including nesting beaches for threatened loggerhead turtles (Caretta caretta). This study 1) measured concentrations of herbicides in the beach sand of Mon Repos, an important marine turtle nesting beach in Australia that is adjacent to significant sugarcane crops, and 2) investigated the toxicity of these herbicides to marine turtles using a cell-based assay...
July 9, 2017: Chemosphere
https://www.readbyqxmd.com/read/28728122/mutagenicity-and-estrogenicity-of-raw-water-and-drinking-water-in-an-industrialized-city-in-the-yangtze-river-delta
#10
Sanhua Xiao, Xuemin Lv, Yifan Zeng, Tao Jin, Lan Luo, Binbin Zhang, Gang Zhang, Yanhui Wang, Lin Feng, Yuan Zhu, Fei Tang
Public concern was aroused by frequently reported water pollution incidents in Taihu Lake and the Yangtze River. The pollution also caught and sustained the attention of the scientific community. From 2010 to 2016, raw water and drinking water samples were continually collected at Waterworks A and B (Taihu Lake) and Waterworks C (Yangtze River). The non-volatile organic pollutants in the water samples were extracted by solid phase extraction. Ames tests and yeast estrogen screen (YES) assays were conducted to evaluate the respective mutagenic and estrogenic effects...
July 14, 2017: Chemosphere
https://www.readbyqxmd.com/read/28728110/development-of-a-bioanalytical-test-battery-for-water-quality-monitoring-fingerprinting-identified-micropollutants-and-their-contribution-to-effects-in-surface-water
#11
Peta A Neale, Rolf Altenburger, Selim Aït-Aïssa, François Brion, Wibke Busch, Gisela de Aragão Umbuzeiro, Michael S Denison, David Du Pasquier, Klára Hilscherová, Henner Hollert, Daniel A Morales, Jiří Novák, Rita Schlichting, Thomas-Benjamin Seiler, Helene Serra, Ying Shao, Andrew J Tindall, Knut Erik Tollefsen, Timothy D Williams, Beate I Escher
Surface waters can contain a diverse range of organic pollutants, including pesticides, pharmaceuticals and industrial compounds. While bioassays have been used for water quality monitoring, there is limited knowledge regarding the effects of individual micropollutants and their relationship to the overall mixture effect in water samples. In this study, a battery of in vitro bioassays based on human and fish cell lines and whole organism assays using bacteria, algae, daphnids and fish embryos was assembled for use in water quality monitoring...
July 9, 2017: Water Research
https://www.readbyqxmd.com/read/28728104/design-synthesis-and-evaluation-of-coumarin-pargyline-hybrids-as-novel-dual-inhibitors-of-monoamine-oxidases-and-amyloid-%C3%AE-aggregation-for-the-treatment-of-alzheimer-s-disease
#12
Hua-Li Yang, Pei Cai, Qiao-Hong Liu, Xue-Lian Yang, Fan Li, Jin Wang, Jia-Jia Wu, Xiao-Bing Wang, Ling-Yi Kong
A series of coumarin-pargyline hybrids (4a-x) have been designed, synthesized and evaluated as novel dual inhibitors of Alzheimer's disease (AD). Most of the compounds exhibited a potent ability to inhibit amyloid-β (Aβ) aggregation and monoamine oxidases. In particular, compound 4x exhibited remarkable inhibitory activities against monoamine oxidases (IC50, 0.027 ± 0.004 μM for MAO-B; 3.275 ± 0.040 μM for MAO-A) and Aβ1-42 aggregation (54.0 ± 1.1%, 25 μM). Moreover, compound 4x showed low toxicity according to in vitro cell toxicity test...
July 8, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28728056/sequence-based-diversity-of-23-autosomal-str-loci-in-koreans-investigated-using-an-in-house-massively-parallel-sequencing-panel
#13
Eun Hye Kim, Hwan Young Lee, So Yeun Kwon, Eun Young Lee, Woo Ick Yang, Kyoung-Jin Shin
As DNA databases continue to grow and international cooperation increases, forensic STR loci have expanded to increase the discriminatory power and inter-database compatibility. Current capillary electrophoresis (CE) and/or massively parallel sequencing (MPS)-based commercial STR analysis systems reflect such changing trends of expanding STR loci. Due to the general gains of larger multiplexing and the detection of sequence variation, the application of MPS technology to STR analysis has further improved discrimination and is expected to aid in mixture interpretation by increasing the effective number of alleles...
July 9, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28728040/measures-and-predictors-of-varenicline-adherence-in-the-treatment-of-nicotine-dependence
#14
Annie R Peng, Mark Morales, E Paul Wileyto, Larry W Hawk, Paul Cinciripini, Tony P George, Neal L Benowitz, Nicole L Nollen, Caryn Lerman, Rachel F Tyndale, Robert Schnoll
INTRODUCTION: While adherence to medication in smoking cessation clinical trials is strongly associated with clinical outcome, very few studies have evaluated the validity of pill count as a measure of adherence relative to a biological assay, and evaluated a broad range of correlates of adherence. METHODS: In a smoking cessation clinical trial of varenicline, we compared pill counts collected over 4 different time periods to varenicline salivary levels taken after 2weeks of treatment, as well as evaluated predictors of adherence to varenicline...
July 12, 2017: Addictive Behaviors
https://www.readbyqxmd.com/read/28728003/evaluation-of-the-effects-of-low-energetic-microwave-irradiation-on-anaerobic-digestion
#15
Bert Bastiaens, Rob Van den Broeck, Lise Appels, Raf Dewil
The present study investigates the effects of microwave irradiation on the performance of anaerobic digestion processes. A first set of experiments is performed to distinguish the upper limit of the applied energy levels. Secondly, the effects of these treatments on the performance of the digestion process are evaluated in 3 experimental setups: (i) monitoring the acetic acid degradation, (ii) performing a biological methane potential (BMP) assay and (iii) conducting a specific methanogenic activity (SMA) test...
July 17, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28727985/comparative-study-and-analytical-verification-of-pcr-methods-for-the-diagnosis-of-congenital-chagas-disease
#16
Carolina Inés Cura, Juan Carlos Ramírez, Marcelo Rodríguez, Constanza Lopez-Albízu, Lucía Irazu, Karenina Scollo, Sergio Sosa-Estani
Congenital infection is currently the first cause of new cases of Chagas disease in Argentina and nonendemic areas worldwide. Its diagnosis is of utmost importance to guarantee curative treatment. To improve such diagnosis, a transfer process of PCR tests to the national laboratory network has been initiated. We performed a comparative study of four PCR assays [two end-point PCR and two duplex real-time quantitative PCR (qPCR) procedures] to detect Trypanosoma cruzi DNA in blood samples. Because satellite DNA and kinetoplastid DNA qPCR methods have the best performance and the use of two different molecular targets for confirmatory purposes has been recommended, these methods selected to perform the transfer process and, in consequence, subjected to an analytical verification protocol based on international guidelines...
July 17, 2017: Journal of Molecular Diagnostics: JMD
https://www.readbyqxmd.com/read/28727940/intestinal-transit-time-and-cortisol-mediated-stress-in-zebrafish
#17
Clayton Brady, Maxwell Denora, Ian Shannon, Karl J Clark, Adam Rich
Intestinal motility, the spontaneous and rhythmic smooth muscle contraction, is a complex process that is regulated by overlapping and redundant regulatory mechanisms. Primary regulators intrinsic to the gastrointestinal tract include interstitial cells of Cajal, enteric neurons, and smooth muscle cells. Extrinsic primary regulators include the autonomic nervous system, immune system, and the endocrine system. Due to this complexity, a reductionist approach may be inappropriate if the ultimate goal is to understand motility regulation in vivo...
July 20, 2017: Zebrafish
https://www.readbyqxmd.com/read/28727918/rapid-sensitive-and-quantitative-detection-of-anti-peg-antibodies-by-mpeg-coated-spr-sensors
#18
Peng Zhang, Fang Sun, Hsiang-Chieh Hung, Priyesh Jain, Kasey Joanne Leger, Shaoyi Jiang
Pre-existing and induced anti-poly(ethylene glycol) (PEG) antibodies (abs) have been shown to be related with limitation of therapeutic efficacy and reduction in tolerance of several therapeutic agents. However, the current methods to detect anti-PEG abs are tedious and usually lack of quantification. A facile, rapid, sensitive and reliable technique to detect anti-PEG abs is highly desired in both research and clinic settings. In this work, we have presented a surface plasmon resonance (SPR) biosensor technique for the detection of anti-PEG abs and compared three PEG surface chemistries...
July 20, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28727913/alpha-l-fucosidase-immunoassay-for-early-detection-of-hepatocellular-carcinoma
#19
Eric Waidely, Abdulrahman Obaid Al-Youbi, Abdulaziz Saleh Omar Bashammakh, Mohammad Soror El-Shahawi, Roger M Leblanc
Detection of alpha-L-fucosidase has been shown to have relevance in diagnosing hepatocellular carcinoma. Few assays have been developed to measure this enzyme, with most relying on colorimetric techniques involving the enzyme's kinetics. While these assays are facile and quick, the sensitivity is not always sufficient for early tumor detection. To improve upon previous assays for alpha-L-fucosidase, a fluorescence based immunoassay was produced implementing an alpha-L-fucosidase specific antibody (FUCA2). The immobilization of the alpha-L-fucosidase-specific antibody onto a quartz slide was investigated with several bioconjugation approaches and an immunoassay for detection of alpha-L-fucosidase was produced...
July 20, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28727855/association-of-apoe-gene-polymorphism-with-lipid-profile-and-coronary-artery-disease-in-afro-caribbeans
#20
Laurent Larifla, Christophe Armand, Jacqueline Bangou, Anne Blanchet-Deverly, Patrick Numeric, Christiane Fonteau, Carl-Thony Michel, Séverine Ferdinand, Véronique Bourrhis, Fritz-Line Vélayoudom-Céphise
OBJECTIVES: Apolipoprotein E gene (APOE) polymorphism is associated with the lipid profile and cardio-vascular disease. However, these relationships vary between ethnic groups. We evaluated, for the first time in an Afro-Caribbean population, the distribution of APOE polymorphisms and their associations with coronary artery disease (CAD), the lipid profile and other cardio-metabolic risk factors. METHODS: We studied 712 Afro-Caribbean subjects including 220 with documented CAD and 492 healthy subjects...
2017: PloS One
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