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https://www.readbyqxmd.com/read/29353432/post-translational-modifications-in-tumor-biomarkers-the-next-challenge-for-aptamers
#1
Ana Díaz-Fernández, Rebeca Miranda-Castro, Noemí de-Los-Santos-Álvarez, María Jesús Lobo-Castañón
Advances in proteomics have fueled the search for novel cancer biomarkers with higher selectivity. Differential expression of low abundant proteins has been the usual way of finding those biomarkers. The existence of a selective receptor for each biomarker is compulsory for their use in diagnostic/prognostic assays. Antibodies are the receptors of choice in most cases although aptamers are becoming familiar because of their facile and reproducible synthesis, chemical stability as well as comparable affinity and selectivity...
January 20, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29353430/multiplex-highly-sensitive-immunochromatographic-assay-based-on-the-use-of-nonprocessed-antisera
#2
Nadezhda A Byzova, Alexandr E Urusov, Anatoly V Zherdev, Boris B Dzantiev
The format of an immunochromatographic multiassay is first proposed with native antisera and a universal conjugate of antispecies antibodies with gold nanoparticles. This format allows (1) the exclusion of purification and conjugation stages for specific antibodies and (2) significant reduction of the concentration of specific antibodies in the system. The independent use of specific antibodies and a conjugated marker provided a low detection limit and high signal intensity. The proposed format was implemented for the simultaneous detection of two herbicides...
January 20, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29353423/polymerase-chain-reaction-based-detection-of-myc-transduction-in-feline-leukemia-virus-infected-cats
#3
Ryosuke Sumi, Ariko Miyake, Taiji Endo, Yoshiharu Ohsato, Minh Ha Ngo, Kazuo Nishigaki
Feline lymphomas are associated with the transduction and activation of cellular proto-oncogenes, such as c-myc, by feline leukemia virus (FeLV). We describe a polymerase chain reaction assay for detection of myc transduction usable in clinical diagnosis. The assay targets c-myc exons 2 and 3, which together result in a FeLV-specific fusion gene following c-myc transduction. When this assay was conducted on FeLV-infected feline tissues submitted for clinical diagnosis of tumors, myc transduction was detected in 14% of T-cell lymphoma/leukemias...
January 20, 2018: Archives of Virology
https://www.readbyqxmd.com/read/29353420/properties-of-antibiotic-resistant-bacteria-isolated-from-onsite-wastewater-treatment-plant-in-relation-to-biofilm-formation
#4
Łukasz Jałowiecki, Joanna Żur, Joanna Chojniak, Helene Ejhed, Grażyna Płaza
The aim of the present study was to determine some properties of antibiotic-resistant bacterial strains isolated from onsite wastewater technology in relation to biofilm formation, e.g., autoaggregation and motility. Additionally, biosurfactant production by the isolates was also evaluated. The ability of selected strains to develop a biofilm was assessed by using the crystal violet method, which allows to indirectly quantify the attached bacterial biomass (live, dead cells, and polysaccharides as well). Obtained results showed that 19 of the analyzed strains were able to produce biofilm after 72 h of incubation...
January 20, 2018: Current Microbiology
https://www.readbyqxmd.com/read/29353403/genetic-polymorphism-in-hsp90aa1-gene-is-associated-with-the-thermotolerance-in-chinese-holstein-cows
#5
T M Badri, K L Chen, M A Alsiddig, Lian Li, Yafei Cai, G L Wang
The heat shock protein 90 (Hsp90) is a copious and ubiquitous molecular chaperone which plays an essential role in many cellular biological processes. The objective of this study was to identify single nucleotide polymorphisms (SNPs) in the Hsp90AA1 gene and to determine their association with heat stress traits in Chinese Holstein cattle breed. Direct sequencing was used to identify new SNPs. Luciferase reporter assay methods were used to assess g.- 87G > C and g.4172A > G loci in the promoter activity and 3'-UTR, respectively...
January 20, 2018: Cell Stress & Chaperones
https://www.readbyqxmd.com/read/29353346/singleplex-quantitative-real-time-pcr-for-the-assessment-of-human-mitochondrial-dna-quantity-and-quality
#6
Corey Goodwin, Denice Higgins, Shanan S Tobe, Jeremy Austin, Andrew Wotherspoon, Michelle E Gahan, Dennis McNevin
Mitochondrial DNA (mtDNA) can provide a means for forensic identity testing when genotyping of nuclear DNA (nuDNA) targets is not possible due to degradation or lack of template. For degraded samples, an indication of the quantity and quality of mtDNA is essential to allow selection of appropriately sized targets for hypervariable region (HVR) analysis, which may conserve sample and resources. Three human-specific mtDNA targets of increasing length (86, 190 and 452 base pairs) were amplified by singleplex quantitative real-time PCR (qPCR), capable of providing an index of mtDNA degradation from fragment length information...
January 20, 2018: Forensic Science, Medicine, and Pathology
https://www.readbyqxmd.com/read/29353259/non-invasive-prenatal-testing-of-pregnancies-at-risk-for-phenylketonuria
#7
Huikun Duan, Ning Liu, Zhenhua Zhao, Yiqian Liu, Yin Wang, Zhifeng Li, Mengnan Xu, David S Cram, Xiangdong Kong
BACKGROUND: Phenylketonuria (PKU) is a common metabolic disorder caused predominately by mutations in the phenylalanine hydroxylase (PAH) gene. The aim of the study was to design and validate the performance of a non-invasive prenatal test (NIPT) for PKU using circulating single molecule amplification and resequencing technology (cSMART). METHODS: A total of 18 couples at genetic risk for having a child with PKU were recruited to the study. Gold standard invasive prenatal diagnosis (IPD) was performed on amniocyte or villus cell DNA by Sanger sequencing, targeting the known parental PAH mutations...
January 20, 2018: Archives of Disease in Childhood. Fetal and Neonatal Edition
https://www.readbyqxmd.com/read/29353208/natural-products-berberine-and-curcumin-exhibited-better-ameliorative-effects-on-rats-with-non-alcohol-fatty-liver-disease-than-lovastatin
#8
Wu-Wen Feng, Shuang-Yu Kuang, Can Tu, Zhi-Jie Ma, Jing-Yao Pang, Yan-Hui Wang, Qing-Ce Zang, Ta-Si Liu, Yan-Ling Zhao, Xiao-He Xiao, Jia-Bo Wang
Studies have shown that satins and herbal products have potential to treat non-alcohol fatty liver disease (NAFLD) in clinic. However, no study has compared their effects, and their mechanisms remain unresolved. Here, we choose lovastatin and two herbal products including berberine and curcumin to compare their effects in treating NAFLD. NAFLD model was established by high fat food, and rats were administrated with lovastatin, berberine, curcumin, berberine + curcumin at the dosage of 100, 100, 100, 50 + 50 mg/kg bw, respectively...
January 17, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29353206/dihydroartemisinin-ameliorates-sepsis-induced-hyperpermeability-of-glomerular-endothelium-via-up-regulation-of-occludin-expression
#9
Zuowang Cheng, Ruixia Qi, Liqun Li, Qiang Liu, Wenqian Zhang, Xia Zhou, Dongmei Xu, Thaddeus D Allen, Silin Pan, Ju Liu
Sepsis, the systemic inflammatory responses after infection, remains a serious cause of morbidity and mortality in critically ill patients. The anti-malarial agent dihydroartemisinin (DHA) has been shown to be anti-inflammatory. In this study, we examined the effects of DHA on sepsis-induced acute kidney injury (AKI) and explored the mechanism underlying its mode of action in AKI. In a lipopolysaccharide (LPS)-induced mouse model, we observed that DHA treatment ameliorated glomerular injury, and relieved elevation of the urine albumin to creatinine ratio (UACR) and serum creatinine...
January 17, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29353202/validation-of-a-traditional-preparation-against-multi-drug-resistant-salmonella-typhi-and-its-protective-efficacy-in-s-typhimurium-infected-mice
#10
Debprasad Chattopadhyay, Durbadal Ojha, Hemanta Mukherjee, Paromita Bag, Shatrughna Prasad Vaidya, Shanta Dutta
Shorea robusta Gaertn has been used for skin and intestinal ailments in Indian Traditional medicine; while two tribal communities used its tender leaves in 'Meyadi-bukhar' or long-term fever. This prompted us to validate the aqueous and methanol extracts of Shorea robusta tender leaves against wild- and multidrug-resistant clinical isolates of Salmonella enterica Serovar Typhi (S. Typhi), the causative agent of typhoid fever. The antibacterial activity, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and growth inhibition were determined using disc diffusion, agar-and-broth dilution, dose- and time-response assays, along with the safety and protective efficacy in Balb/C mice, infected with S...
January 17, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29353197/bioanalytical-assessment-of-adaptive-stress-responses-in-drinking-water-a-predictive-tool-to-differentiate-between-micropollutants-and-disinfection-by-products
#11
Armelle Hebert, Cedric Feliers, Caroline Lecarpentier, Peta A Neale, Rita Schlichting, Sylvie Thibert, Beate I Escher
Drinking water can contain low levels of micropollutants, as well as disinfection by-products (DBPs) that form from the reaction of disinfectants with organic and inorganic matter in water. Due to the complex mixture of trace chemicals in drinking water, targeted chemical analysis alone is not sufficient for monitoring. The current study aimed to apply in vitro bioassays indicative of adaptive stress responses to monitor the toxicological profiles and the formation of DBPs in three drinking water distribution systems in France...
January 3, 2018: Water Research
https://www.readbyqxmd.com/read/29353190/characterization-of-acrylamide-induced-oxidative-stress-and-cardiovascular-toxicity-in-zebrafish-embryos
#12
Mengmeng Huang, Jingjing Jiao, Jun Wang, Zhidan Xia, Yu Zhang
Acrylamide (AA) is a high production volume chemical in industrial applications and widely found in baked or fried carbohydrate-rich foods. In this study, we unravelled that AA induced developmental toxicity associated with oxidative stress status and disordered lipid distribution in heart region of developing zebrafish. Treatment with AA caused a deficient cardiovascular system with significant heart malformation and dysfunction. We also found that AA could reduce the number of cardiomyocytes through the reduced capacity of cardiomyocyte proliferation rather than cell apoptosis...
January 8, 2018: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/29353188/mechanism-of-anaerobic-bio-reduction-of-azo-dye-assisted-with-lawsone-immobilized-activated-carbon
#13
Daniel Olivo-Alanis, Refugio Bernardo Garcia-Reyes, Luis H Alvarez, Alcione Garcia-Gonzalez
Lawsone redox (LQ) mediator was covalently bound to granular activated carbon (GAC) by Fischer esterification. A high LQ adsorption capacity on GAC was achieved (∼230 mg/g), and desorption studies showed strong chemical stability. Furthermore, kinetic experiments with solid-phase redox mediator (RM) and their controls (soluble RM, GAC and anaerobic sludge) were tested for decolorization of congo red dye at initial concentration of 175 mg/L. Benzidine, a by-product of complete congo red reduction, was also measured by HPLC analysis along the kinetic experiments...
January 9, 2018: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/29353171/microcystin-lr-nanobody-screening-from-an-alpaca-phage-display-nanobody-library-and-its-expression-and-application
#14
Chongxin Xu, Ying Yang, Liwen Liu, Jianhong Li, Xiaoqin Liu, Xiao Zhang, Yuan Liu, Cunzheng Zhang, Xianjin Liu
Microcystin-LR (MC-LR) is a type of biotoxin that pollutes the ecological environment and food. The study aimed to obtain new nanobodies from phage nanobody library for determination of MC-LR. The toxin was conjugated to keyhole limpet haemocyanin (KLH) and bovine serum albumin (BSA), respectively, then the conjugates were used as coated antigens for enrichment (coated MC-LR-KLH) and screening (coated MC-LR-BSA) of MC-LR phage nanobodies from an alpaca phage display nanobody library. The antigen-specific phage particles were enriched effectively with four rounds of biopanning...
January 17, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29353169/integrated-biomarkers-response-confirm-the-antioxidant-role-of-diphenyl-diselenide-against-atrazine
#15
Aline Teixeira Marins, Cíntia Côrte Real Rodrigues, Charlene Cavalheiro de Menezes, Jeane de Lima Costa Gomes, Maiara Dorneles Costa, Mauro Eugênio Medina Nunes, Mariela de Souza Vieira, Filipe Fagan Donato, Renato Zanella, Leila Picolli da Silva, Vania Lucia Loro
Atrazine (ATZ) is a herbicide worldwide used. That can cause oxidative damage in non-target organisms, such as fish. Furthermore, the threat of exposure to pesticides together with poor nutrition is hazardous to the normal development of fish, and supplementation of the fish diet with antioxidants compounds is an alternative approach to prevent the hazardous effects of pesticide exposure. Here we aimed to investigate the capacity of diphenyl diselenide (PhSe)2 diet supplementation to improve the antioxidant defense of Cyprinus carpio (carp) exposed to environmental concentrations of ATZ...
January 16, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29353152/tgf%C3%AE-c-509t-tgf%C3%AE-t869c-xrcc1-arg194trp-ikb%C3%AE-c642t-il4-c-590t-genetic-polymorphisms-combined-with-socio-economic-lifestyle-diet-factors-and-gastric-cancer-risk-a-case-control-study-in-south-indian-population
#16
Dhayalan Pavithra, Majumdar Gautam, Ranganathan Rama, Rajaraman Swaminathan, Gopisetty Gopal, Ayyalur Seshadri Ramakrishnan, Thangarajan Rajkumar
BACKGROUND: Gastric cancer is worldwide the third major cause of cancer related death. Risk factors for gastric cancer includes Helicobacter pylori infection, gastric ulcer, less hygienic condition, use of tobacco, alcohol consumption, use of salted, smoked food, genetic alterations etc. In order to identify the risk factors associated with gastric cancer in South Indian population a case-control study involving 200 proven gastric cancer cases and 400 controls was conducted. METHODS: A structured questionnaire was used to interview all the subjects who participated in our study...
January 17, 2018: Cancer Epidemiology
https://www.readbyqxmd.com/read/29353142/mirna-185-regulates-the-vegfa-signaling-pathway-in-dairy-cows-with-retained-fetal-membranes
#17
REVIEW
C Y Zheng, X Zou, H J Lin, B C Zhao, M L Zhang, C H Luo, S X Fu
Retention of fetal membranes (RFM) of cows is an important reproductive disturbance, and is related to miRNAs. Vascular endothelial growth factor (VEGF)A, regulated by miRNA-185, can activate arachidonic acid (ARA) release via the VEGFA signaling pathway, which influences RFM. The aim of this study was to explore the pathogenic mechanism of RFM by investigating the regulatory relationship between miRNA-185 and the VEGFA signaling pathway. Serum samples of healthy Holstein dairy cows (n = 20) and RFM cows (n = 12), with a similar age, parity, weight, and milk yield, were collected to detect VEGFA and ARA concentrations at 6, 12, and 24 h after calving...
January 9, 2018: Theriogenology
https://www.readbyqxmd.com/read/29353137/assessment-of-the-cytotoxicity-of-ionic-liquids-on-spodoptera-frugiperda-9-sf-9-cell-lines-via-in-vitro-assays
#18
Shuanggen Wu, Liangbin Zeng, Chaoyun Wang, Yuanru Yang, Wanlai Zhou, Fenfang Li, Zhijian Tan
Cytotoxicity studies are important tools for the assessment of the toxicity of ionic liquids (ILs). In the present study, the cytotoxicity of eleven ILs against Spodoptera frugiperda 9 (Sf-9) cell lines were evaluated via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays. The effect on cellular morphology, ultrastructural morphology, and nuclear morphology induced by 1-ethyl-3-methylimidazolium bromide ([C2mim][Br]) was studied via inverted light microscopy observation, acridine orange staining, and transmission electron microscope (TEM) analysis, respectively...
January 17, 2018: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/29353135/copper-induces-expression-and-methylation-changes-of-early-development-genes-in-crassostrea-gigas-embryos
#19
Rossana Sussarellu, Morgane Lebreton, Julien Rouxel, Farida Akcha, Guillaume Rivière
Copper contamination is widespread along coastal areas and exerts adverse effects on marine organisms such as mollusks. In the Pacific oyster, copper induces severe developmental abnormalities during early life stages; however, the underlying molecular mechanisms are largely unknown. This study aims to better understand whether the embryotoxic effects of copper in Crassostrea gigas could be mediated by alterations in gene expression, and the putative role of DNA methylation, which is known to contribute to gene regulation in early embryo development...
January 3, 2018: Aquatic Toxicology
https://www.readbyqxmd.com/read/29353083/combining-ultrasound-and-intratumoral-administration-of-doxorubicin-loaded-microspheres-to-enhance-tumor-cell-killing
#20
Anh-Vu Do, Sean M Geary, Dongrim Seol, Phillip Tobias, Daniel Carlsen, Nattawut Leelakanok, James A Martin, Aliasger K Salem
Melanoma is an incurable disease for which alternative treatments to chemotherapy alone are sought. Here, using a melanoma model, we investigated the antitumor potential of combining ultrasound (US) with poly(lactic-co-glycolic acid) (PLGA) microspheres loaded with doxorubicin (DOX). The aim was to achieve synergistic tumoricidal activity through direct and indirect US-mediated damage of tumor cells combined with sustained and potentially controllable release (when combined with US) of DOX from microspheres...
January 15, 2018: International Journal of Pharmaceutics
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