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https://www.readbyqxmd.com/read/27927008/microrna-296-5p-promotes-invasiveness-through-downregulation-of-nerve-growth-factor-receptor-and-caspase-8
#1
Hong Lee, Chang Hoon Shin, Hye Ree Kim, Kyung Hee Choi, Hyeon Ho Kim
Glioblastomas (GBM) are very difficult to treat and their aggressiveness is one of the main reasons for this as well as for the frequent recurrences. MicroRNAs posttranscriptionally regulate their target genes through interaction between their seed sequence and 3'UTR of the target mRNAs. We previously reported that miR-296-3p is regulated by neurofibromatosis 2 (NF2) and enhances the invasiveness of GBM cells via SOCS2/STAT3. In this study, we investigated whether miR-296-5p, which originates from the same precursor miRNA as miR-296-3p, can increase the invasiveness of GBM cells...
December 8, 2016: Molecules and Cells
https://www.readbyqxmd.com/read/27926935/serological-and-virological-evidence-of-crimean-congo-haemorrhagic-fever-virus-circulation-in-the-human-population-of-borno-state-northeastern-nigeria
#2
David N Bukbuk, Stuart D Dowall, Kuiama Lewandowski, Andrew Bosworth, Saka S Baba, Anitha Varghese, Robert J Watson, Andrew Bell, Barry Atkinson, Roger Hewson
BACKGROUND: Despite several studies on the seroprevalence of antibodies against Crimean-Congo Haemorrhagic Fever virus (CCHFV) from humans and cattle in Nigeria, detailed investigation looking at IgG and IgM have not been reported. Additionally, there have been no confirmed cases of human CCHFV infection reported from Nigeria. PRINCIPAL FINDINGS: Samples from sera (n = 1189) collected from four Local Government Areas in Borno State (Askira/Uba, Damboa, Jere and Maiduguri) were assessed for the presence of IgG and IgM antibodies...
December 2016: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/27926925/dengue-in-the-middle-east-and-north-africa-a-systematic-review
#3
John M Humphrey, Natalie B Cleton, Chantal B E M Reusken, Marshall J Glesby, Marion P G Koopmans, Laith J Abu-Raddad
BACKGROUND: Dengue virus (DENV) infection is widespread and its disease burden has increased in past decades. However, little is known about the epidemiology of dengue in the Middle East and North Africa (MENA). METHODOLOGY / PRINCIPAL FINDINGS: Following Cochrane Collaboration guidelines and reporting our findings following PRISMA guidelines, we systematically reviewed available records across MENA describing dengue occurrence in humans (prevalence studies, incidence studies, and outbreak reports), occurrence of suitable vectors (Aedes aegypti and Aedes albopictus), and DENV vector infection rates...
December 2016: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/27926864/low-cd38-identifies-progenitor-like-inflammation-associated-luminal-cells-that-can-initiate-human-prostate-cancer-and-predict-poor-outcome
#4
Xian Liu, Tristan R Grogan, Haley Hieronymus, Takao Hashimoto, Jack Mottahedeh, Donghui Cheng, Lijun Zhang, Kevin Huang, Tanya Stoyanova, Jung Wook Park, Ruzanna O Shkhyan, Behdokht Nowroozizadeh, Matthew B Rettig, Charles L Sawyers, David Elashoff, Steve Horvath, Jiaoti Huang, Owen N Witte, Andrew S Goldstein
Inflammation is a risk factor for prostate cancer, but the mechanisms by which inflammation increases that risk are poorly understood. Here, we demonstrate that low expression of CD38 identifies a progenitor-like subset of luminal cells in the human prostate. CD38(lo) luminal cells are enriched in glands adjacent to inflammatory cells and exhibit epithelial nuclear factor κB (NF-κB) signaling. In response to oncogenic transformation, CD38(lo) luminal cells can initiate human prostate cancer in an in vivo tissue-regeneration assay...
December 6, 2016: Cell Reports
https://www.readbyqxmd.com/read/27926843/membrane-anchoring-and-ion-entry-dynamics-in-p-type-atpase-copper-transport
#5
Christina Grønberg, Oleg Sitsel, Erik Lindahl, Pontus Gourdon, Magnus Andersson
Cu(+)-specific P-type ATPase membrane protein transporters regulate cellular copper levels. The lack of crystal structures in Cu(+)-binding states has limited our understanding of how ion entry and binding are achieved. Here, we characterize the molecular basis of Cu(+) entry using molecular-dynamics simulations, structural modeling, and in vitro and in vivo functional assays. Protein structural rearrangements resulting in the exposure of positive charges to bulk solvent rather than to lipid phosphates indicate a direct molecular role of the putative docking platform in Cu(+) delivery...
December 6, 2016: Biophysical Journal
https://www.readbyqxmd.com/read/27926836/probing-small-molecule-binding-to-unfolded-polyprotein-based-on-its-elasticity-and-refolding
#6
Ricksen S Winardhi, Qingnan Tang, Jin Chen, Mingxi Yao, Jie Yan
Unfolded protein, a disordered structure found before folding of newly synthesized protein or after protein denaturation, is a substrate for binding by many cellular factors such as heat-stable proteins, chaperones, and many small molecules. However, it is challenging to directly probe such interactions in physiological solution conditions because proteins are largely in their folded state. In this work we probed small molecule binding to mechanically unfolded polyprotein using sodium dodecyl sulfate (SDS) as an example...
December 6, 2016: Biophysical Journal
https://www.readbyqxmd.com/read/27926824/identification-and-characterization-of-chemosensors-for-d-malate-unnatural-enantiomer-of-malate-in-ralstonia-pseudosolanacearum
#7
Mattana Tunchai, Akiko Hida, Shota Oku, Yutaka Nakashimada, Takahisa Tajima, Junichi Kato
Ralstonia pseudosolanacearum Ps29 is attracted by nonmetabolizable D-malate, an unnatural enantiomer. Screening of a complete collection of single-mcp-gene deletion mutants of Ps29 revealed that the RSc1156 homolog is a chemosensor for D-malate. An RSc1156 homolog deletion mutant of Ps29 showed decreased but significant responses to D-malate, suggesting the existence of another D-malate chemosensor. McpM previously had been identified as a chemosensor for L-malate. We constructed an RSc1156 homolog mcpM double deletion mutant and noted that this mutant failed to respond to D-malate; thus, the RSc1156 homolog and McpM are the major chemosensors for D-malate in this organism...
December 2, 2016: Microbiology
https://www.readbyqxmd.com/read/27926815/identification-of-lysophospholipase-protein-from-spiroplasma-eriocheiris-and-verification-of-its-function
#8
Huanxi Zhu, Peng Liu, Jie Du, Jian Wang, Yunting Jing, Jia Zhang, Wei Gu, Wen Wang, Qingguo Meng
Spiroplasma eriocheiris is known to cause the tremor disease in the Chinese mitten crab Eriocheir sinensis; however, the molecular characterization of this pathogen is still unclear. S. eriocheiris has the ability to invade and survive within mouse 3T6 cells. The invasion process may require causing damage to the host cell membrane by chemical, physical, or enzymatic means. In this study, we systematically characterized a novel lysophospholipase (lysoPL) of S. eriocheiris TDA-040725-5T. The gene that encodes lysoPL in S...
December 7, 2016: Microbiology
https://www.readbyqxmd.com/read/27926787/comparison-of-the-effects-of-a-balanced-crystalloid-based-and-a-saline-based-tetrastarch-solution-on-canine-whole-blood-coagulation-and-platelet-function
#9
Annina Reuteler, Shannon Axiak-Flammer, Judith Howard, Katja-Nicole Adamik
OBJECTIVE: To evaluate the effects of a 6% hydroxyethyl starch (130/0.42) solution in either a buffered, electrolyte-balanced (HES-BAL), or a saline (HES-SAL) carrier solution on canine platelet function and whole blood coagulation. DESIGN: Prospective, randomized study. SETTING: University teaching hospital. ANIMALS: Thirty-seven client-owned dogs undergoing general anesthesia for arthroscopy or imaging studies. INTERVENTIONS: Dogs received a 15 mL/kg intravenous bolus of HES-SAL (n = 13), HES-BAL (n = 14), or a modified Ringer's solution (n = 10) over 30-40 minutes...
December 7, 2016: Journal of Veterinary Emergency and Critical Care
https://www.readbyqxmd.com/read/27926664/absence-of-serological-rheumatoid-arthritis-biomarkers-in-inflammatory-bowel-disease-patients-with-arthropathies
#10
Sanne J H Van Erp, Marije K Verheul, E W Nivine Levarht, Johan J van der Reijden, Désirée van der Heijde, Floris A van Gaalen, Daniel W Hommes, Gary L Norman, Zakera Shums, Michael Mahler, Hein W Verspaget, Leendert A Trouw, Andrea E van der Meulen-de Jong
OBJECTIVE: Biomarkers that are associated with future progression to rheumatoid arthritis (RA) and joint destruction have been discovered previously in patients with arthralgia. The present study examined these RA biomarkers in inflammatory bowel disease (IBD) patients with arthropathies. PATIENTS AND METHODS: Sera from 155 IBD patients with and 99 IBD patients without arthropathies were analyzed for immunoglobulin (Ig) M rheumatoid factor (RF), IgA-RF, anti-cyclic citrullinated peptide 2, anti-cyclic citrullinated peptide 3...
December 6, 2016: European Journal of Gastroenterology & Hepatology
https://www.readbyqxmd.com/read/27926582/differential-procoagulant-activity-of-microparticles-derived-from-monocytes-granulocytes-platelets-and-endothelial-cells-impact-of-active-tissue-factor
#11
Olga N Shustova, Olga A Antonova, Nina V Golubeva, Svetlana G Khaspekova, Vladimir V Yakushkin, Svetlana A Aksuk, Irina B Alchinova, Mikhail Y Karganov, Alexey V Mazurov
Microparticles released by activated/apoptotic cells exhibit coagulation activity as they express phosphatidylserine and some of them - tissue factor. We compared procoagulant properties of microparticles from monocytes, granulocytes, platelets and endothelial cells and assessed the impact of tissue factor in observed differences. Microparticles were sedimented (20 000g, 30 min) from the supernatants of activated monocytes, monocytic THP-1 cells, granulocytes, platelets and endothelial cells. Coagulation activity of microparticles was examined using plasma recalcification assay...
December 6, 2016: Blood Coagulation & Fibrinolysis: An International Journal in Haemostasis and Thrombosis
https://www.readbyqxmd.com/read/27926517/suberoylanilide-hydroxamic-acid-induced-specific-epigenetic-regulation-controls-leptin-induced-proliferation-of-breast-cancer-cell-lines
#12
Xiuyan Feng, Han Han, Dan Zou, Jiaming Zhou, Weiqiang Zhou
Breast cancer is one of the most common malignancies among women in the world, investigating the characteristics and special transduction pathways is important for better understanding breast development and tumorigenesis. Leptin, a peptide hormone secreted from white adipocytes, may be an independent risk factor for breast cancer.Here, we treated suberoylanilide hydroxamic acid (SAHA) on Leptin-induced cell proliferation and invasion in the estrogen-receptor-positive breast cancer cell line MCF-7 and triple-negative breast cancer cell line MDA-MB-231...
December 1, 2016: Oncotarget
https://www.readbyqxmd.com/read/27926505/antitumor-effect-of-combination-of-the-inhibitors-of-two-new-oncotargets-proton-pumps-and-reverse-transcriptase
#13
Luana Lugini, Ilaria Sciamanna, Cristina Federici, Elisabetta Iessi, Enrico Pierluigi Spugnini, Stefano Fais
Tumor therapy needs new approaches in order to improve efficacy and reduce toxicity of the current treatments. The acidic microenvironment and the expression of high levels of endogenous non-telomerase reverse transcriptase (RT) are common features of malignant tumor cells. The anti-acidic proton pump inhibitor Lansoprazole (LAN) and the non-nucleoside RT inhibitor Efavirenz (EFV) have shown independent antitumor efficacy. LAN has shown to counteract drug tumor resistance. We tested the hypothesis that combination of LAN and EFV may improve the overall antitumor effects...
December 3, 2016: Oncotarget
https://www.readbyqxmd.com/read/27926504/the-immunomodulatory-effects-of-tnf-%C3%AE-inhibitors-on-human-th17-cells-via-ror%C3%AE-t-histone-acetylation
#14
Yi-Ching Lin, Yu-Chih Lin, Cheng-Chin Wu, Ming-Yii Huang, Wen-Chan Tsai, Chih-Hsing Hung, Po-Lin Kuo
The presence of interleukin (IL)-17-related cytokines correlates with rheumatoid arthritis (RA) pathogenesis. Epigenetic modifications, including histone acetylation, regulate gene expression in RA pathogenesis. Tumour necrosis factor-alpha (TNF-α) inhibitors such as etanercept and adalimumab, represent a breakthrough in RA treatment. We aimed to investigate the effects of etanercept and adalimumab on human Th17-polarized cells and the possible intracellular regulators of these effects, including the Th17-specific transcription factors signal transducer, activator of transcription 3 (STAT3), retinoid-related orphan receptor γ-T (RORγt) and epigenetic modification...
December 3, 2016: Oncotarget
https://www.readbyqxmd.com/read/27926502/mir-433-3p-suppresses-cell-growth-and-enhances-chemosensitivity-by-targeting-creb-in-human-glioma
#15
Shupeng Sun, Xiuyu Wang, Xinnv Xu, Hui Di, Jixiang Du, Bin Xu, Qiong Wang, Jinhuan Wang
Previous studies reported that miR-433 exerts function widely in human tumorigenesis and development. Here, we further investigate the potential role of miR-433 in glioma. Quantitative real-time PCR demonstrated that miR-433-3p and miR-433-5p were low expressed in glioma tissues and cell lines. Functional studies suggested that the overexpression of miR-433-3p suppressed proliferation, induced apoptosis and inhibited invasion and migration of human glioma cells. But the growth and metastasis of glioma cells were not significantly influenced by overexpression of miR-433-5p...
December 3, 2016: Oncotarget
https://www.readbyqxmd.com/read/27926488/blocking-ezh2-methylation-transferase-activity-by-gsk126-decreases-stem-cell-like-myeloma-cells
#16
Delong Zeng, Maoxing Liu, Jingxuan Pan
EZH2 is a critical epigenetic regulator that is deregulated in various types of cancers including multiple myeloma (MM). In the present study, we hypothesized that targeting EZH2 might induce apoptosis in myeloma cells including stem cell-like cells (CSCs). We investigated the effect of EZH2 inhibition on MM cells using a potent inhibitor (GSK126). The results showed that GSK126 effectively abrogated the methylated histone 3 (H3K27me3) level in MM.1S and LP1 cells, and inhibited the number of live cells and colony formation in soft agar of six MM cell lines...
December 2, 2016: Oncotarget
https://www.readbyqxmd.com/read/27926480/calcium-dependent-binding-of-myc-to-calmodulin
#17
Philipp Raffeiner, Andrea Schraffl, Thomas Schwarz, Ruth Röck, Karin Ledolter, Markus Hartl, Robert Konrat, Eduard Stefan, Klaus Bister
The bHLH-LZ (basic region/helix-loop-helix/leucine zipper) oncoprotein Myc and the bHLH-LZ protein Max form a binary transcription factor complex controlling fundamental cellular processes. Deregulated Myc expression leads to neoplastic transformation and is a hallmark of most human cancers. The dynamics of Myc transcription factor activity are post-translationally coordinated by defined protein-protein interactions. Here, we present evidence for a second messenger controlled physical interaction between the Ca2+ sensor calmodulin (CaM) and all Myc variants (v-Myc, c-Myc, N-Myc, and L-Myc)...
December 1, 2016: Oncotarget
https://www.readbyqxmd.com/read/27926476/mitochondrial-cytochrome-c-oxidase-is-inhibited-by-atp-only-at-very-high-atp-adp-ratios
#18
Rabia Ramzan, Andreas K Schaper, Petra Weber, Annika Rhiel, Muhammad Saad Siddiq, Sebastian Vogt
In the past, divergent results have been reported based on different methods and conditions used for enzymatic activity measurements of cytochrome c oxidase (CytOx). Here, we analyze in detail and show comparable and reproducible polarographic activity measurements of ATP- dependent inhibition of CytOx kinetics in intact and non-intact rat heart mitochondria and mitoplasts. We found that this mechanism is always present in isolated rat heart mitochondria and mitoplasts; however, it is measurable only at high ATP/ADP ratios using optimal protein concentrations...
December 7, 2016: Biological Chemistry
https://www.readbyqxmd.com/read/27925693/identification-of-n-arylated-nh125-analogues-as-rapid-eradicating-agents-against-mrsa-persister-cells-and-potent-biofilm-killers-of-gram-positive-pathogens
#19
Yasmeen Abouelhassan, Akash Basak, Hussain Yousaf, Robert William Huigens Iii
Bacterial biofilms housing dormant persister cells are innately tolerant to antibiotics and disinfectants, yet several membrane-active agents are known to eradicate tolerant bacterial cells. NH125, a membrane-active persister-killer and starting point for development, led to the identification of N-arylated analogues 1 and 2 and displayed improved biofilm-killing potencies and rapid persister-killing activities in stationary cultures of methicillin-resistant Staphylococcus aureus (MRSA). We found 1 and 2 to be superior to other membrane-active agents in biofilm eradication assays with 1 demonstrating minimum biofilm eradication concentrations (MBEC) of 23...
December 7, 2016: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/27925685/in-vitro-characterization-of-synthoplate-%C3%A2-synthetic-platelet-technology-and-its-in-vivo-evaluation-in-severely-thrombocytopenic-mice
#20
Meenal Shukla, Ujjal D S Sekhon, Venkaiah Betapudi, Wei Li, DaShawn A Hickman, Christa L Pawlowski, Mitchell R Dyer, Matthew D Neal, Keith R McCrae, Anirban Sen Gupta
BACKGROUND: Platelet transfusion applications face severe challenges due to the limited availability and portability, high risk of contamination and short shelf-life of platelets. Therefore there is significant interest in synthetic platelet substitutes that can render hemostasis while avoiding these issues. Platelets promote hemostasis by injury site-selective adhesion and aggregation, and propagation of coagulation reactions on their membrane. Based on these mechanisms, we have developed a synthetic platelet technology (SynthoPlate(™) ) that integrates platelet-mimetic site-selective 'adhesion' and 'aggregation' functionalities via heteromultivalent surface-decoration of lipid vesicles with Von Willebrand Factor-binding, collagen-binding and active platelet integrin GPIIb-IIIa-binding peptides...
December 7, 2016: Journal of Thrombosis and Haemostasis: JTH
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