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Siemon H S Ng, Ali Azizi, Kerrie Edamura, Rebecca J Malott, Robert L Charlebois, Carine Logvinoff, Martha Schreiber, Laurent Mallet, Lucy Gisonni-Lex
UNLABELLED: To compare the performances of conventional in vitro indicator cell culture, quantitative polymerase chain reaction (qPCR), and next-generation sequencing (NGS) as detection methods for adventitious agents, a preliminary assessment was performed using human cytomegalovirus (HCMV) as a model virus. HCMV was spiked into a crude viral harvest at various concentrations and inoculated onto MRC-5 cell monolayers. The cultures were observed for cytopathic effects (CPEs) as per the compendial method requirements, and samples were taken at various time points for analysis by qPCR or NGS...
November 2014: PDA Journal of Pharmaceutical Science and Technology
Yue Song, Xing Xin, Zhijun Xia, Xingyue Zhai, Keng Shen
The objective of our study was to construct recombinant adenovirus (rAd) AdHTVP2G5-rev-casp3, which expresses autocatalytic caspase-3 driven by human telomerase reverse transcriptase promoter (hTERTp) with a two-step transcription amplification (TSTA) system and investigate its antitumor effects on ovarian cancer in vitro and in vivo. Fluorescent detection was used to detect EGFP expression in various cells. Cell viabilities were determined using the Cell Counting Kit-8 and flow cytometry. RT-PCR and immunoblotting assays were used to detect cellular apoptotic activities...
July 2014: Oncology Reports
Wei Wang, Shan Zheng, Zai Shong, Rui Zhao
To explore the possible link between perinatal cytomegalovirus infection and onset of biliary atresia, we build up a perinatal cytomeglovirus induced hepatobiliary system injury model in guinea pigs, verified the biliary tropism of gpCMV and the related inflammatory injury. It suggested host immune status, local response to inflammation, and activation of Th1-cell-mediated antiviral immune responses contributed to newborn geinea pigs' hepatobiliary damage. The model was helpful in study the CMV infection and biliary injury...
2011: Fetal and Pediatric Pathology
Maha I S Kawashti, Omnia R Amin, Nadia G Rowehy
Autism is a pervasive developmental disorder that affect children early in their life. Immunological disorders is one of several contributing factors that have been suggested to cause autism. Thirty autistic children aged 3-6 years and thirty non-autistic psychologically-free siblings were studied. Circulating IgA and IgG autoantibodies to casein and gluten dietary proteins were detected by enzyme-immunoassays (EIA). Circulating IgG antibodies to measles, mumps and rubella vaccine (M.M.R) and cytomeglovirus were investigated by EIA...
2006: Egyptian Journal of Immunology
Sara Borniquel, Inmaculada Valle, Susana Cadenas, Santiago Lamas, MarĂ­a Monsalve
Nitric oxide (NO) has both prooxidant and antioxidant activities in the endothelium; however, the molecular mechanisms involved are still a matter of controversy. PGC-1alpha [peroxisome proliferators-activated receptor (PPAR) gamma coactivator 1-alpha] induces the expression of several members of the mitochondrial reactive oxygen species (ROS) detoxification system. Here, we show that NO regulates this system through the modulation of PGC-1alpha expression. Short-term (<12 h) treatment of endothelial cells with NO donors down-regulates PGC-1alpha expression, whereas long-term (>24 h) treatment up-regulates it...
September 2006: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Tsukasa Ohmori, Jun Mimuro, Katsuhiro Takano, Seiji Madoiwa, Yuji Kashiwakura, Akira Ishiwata, Masanori Niimura, Katsuyuki Mitomo, Toshiaki Tabata, Mamoru Hasegawa, Keiya Ozawa, Yoichi Sakata
Platelets release several mediators that modify vascular integrity and hemostasis. In the present study, we developed a technique for efficient transgene expression in platelets in vivo and examined whether this targeted-gene-product delivery system using a platelet release reaction could be exploited for clinical applications. Analysis of luciferase reporter gene constructs driven by platelet-specific promoters (the GPIIb, GPIbalpha, and GPVI) revealed that the GPIbalpha promoter was the most potent in the megakaryoblastic cell line UT-7/TPO and human CD34+-derived megakaryocytes...
July 2006: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Chao-man He, Liang-zhen Wen
OBJECTIVE: To investigate the difference of anticytomegaloviral activity of three kinds of traditional Chinese medicines which are the injections of Jinye Baidu, Radix Isatidis and Indigowoa in vitro. METHOD: The inhibitory activity of three traditional Chinese medicines against human cytomeglovirus (HCMV AD169) infected human embryo lung fibroblasts (HELF) was observed by cytopathic effect method (CPE) and MTT method in vitro. According their value of A, anticytomegaloviral activity has evaluated...
May 2004: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
J Tang, Y Sun, Z Meng, A Liu, J Wang, C Wang, C Tian, J Niu, S Chen, X Dong
64 cases of blood recipients were followed-up for 6 months to study infection of hepatitis C virus. The infection rate of hepatitis C virus was 18.75% in total, including 8 cases of post-transfusion hepatitis C (PT-HC), 1 case of subclinical PT-HC, and 3 cases of anti-HCV sero conversion only. The time of initial ALT abnormality and anti-HCV sero conversion were 28 to 91 (51.9 +/- 20.9) days and 23 to 76 (42.4 +/- 15.9) days after blood transfusion respectively. There were 2 cases with cytomeglovirus infection and another 5 cases with unknown infectious agent were found in this study...
March 1997: Chinese Journal of Experimental and Clinical Virology
F Qiu, R Lang, X Yang
OBJECTIVE: To study the prophylaxis, diagnosis and treatment of cytomegalovirus (CMV) infection after liver transplantation. METHODS: The clinical data of 10 patients receiving liver transplantation from July 1999 to October 2000 in our hospital were retrospectively analyzed along with literature review. RESULTS: CMV infection occurred asymptomatic cally in 6 patients, of whom, 5 were cured. CONCLUSIONS: Fluorogenic quantitative PCR (FQ-PCR) is effective, sensitive and highly specific for detecting CMV infection...
September 2001: Zhonghua Wai Ke za Zhi [Chinese Journal of Surgery]
W B Goggins, D M Finkelstein
This paper focuses on the methodology developed for analyzing a multivariate interval-censored data set from an AIDS observational study. A purpose of the study was to determine the natural history of the opportunistic infection cytomeglovirus (CMV) in an HIV-infected individual. For this observational study, laboratory tests were performed at scheduled clinic visits to test for the presence of the CMV virus in the blood and in the urine (called CMV shedding in the blood and urine). The study investigators were interested in determining whether the stage of HIV disease at study entry was predictive of an increased risk for CMV shedding in either the blood or the urine...
September 2000: Biometrics
J Baillargeon, J Grady, M J Borucki
While the prognostic role of CD4 lymphocyte count is well established, little is known about how other immunological factors are associated with HIV-related survivorship. CD8 lymphocyte count is reported to inhibit growth of HIV in vitro and to deter the development of cytomeglovirus infections. Scarce information exists, however, on its association with HIV-related survivorship. The objective of the present study therefore was to assess whether survival estimation could be improved by including CD8 lymphocyte count as a prognostic factor...
July 1999: International Journal of STD & AIDS
Nesmith, Pass
The incidence of cytomeglovirus in adolescents is high, and congenital CMV infection is a major problem for infants born to teenaged mothers. The authors review the biology, epidemiology, clinical manifestations, diagnosis, treatment, and prevention of CMV infection.
February 1995: Adolescent Medicine
E C Dunkel, D I Scheer, Q Zhu, R J Whitley, P A Schaffer, D Pavan-Langston, R J Whitely
No abstract text is available yet for this article.
June 1998: Journal of Infectious Diseases
M J Farthing, M P Kelly, A M Veitch
Persistent diarrhoea and small bowel enteropathy are important features of HIV infection. At least 80% of cases of persistent diarrhoea in patients with HIV/AIDS can be attributed to a specific enteropathogen. The coccidian parasites Cryptosporidium parvum, Isospora belli and Cyclospora and the Microsporidia account for at least 50% of cases of persistent diarrhoea in the industrialised and developing world with major contributions from Mycobacterium avium complex and other bacteria and cytomeglovirus. The intracellular protozoa can be detected on faecal microscopy although confirmation may be required by intestinal mucosal biopsies...
May 1996: Journal of Antimicrobial Chemotherapy
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