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https://www.readbyqxmd.com/read/28535039/deposition-of-gold-nanotriangles-in-large-scale-close-packed-monolayers-for-x-ray-based-temperature-calibration-and-sers-monitoring-of-plasmon-driven-catalytic-reactions
#1
Ferenc Liebig, Radwan M Sarhan, Mathias Sander, Wouter A Koopman, Roman Schuetz, Matias Bargheer, Joachim Koetz
Anisotropic plasmonic particles such as gold nanotriangles have extraordinary structural, optical and physico-chemical properties. For many applications in different fields it is essential to prepare them in a chemically and physically stable, structurally well-defined manner, e.g., as large and uniform coverage on a substrate. We present a direct method for the large scale close-packed monolayer formation of edge-to-edge ordered, ultrathin crystalline gold nanotriangles on Si-wafers or quartz glass via the transfer of these asymmetric particles to the air-liquid interface after adding ethanol-toluene mixtures without any subsequent surface functionalization...
May 23, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28534612/sers-activated-platforms-for-immunoassay-probes-encoding-methods-and-applications
#2
Zhuyuan Wang, Shenfei Zong, Lei Wu, Dan Zhu, Yiping Cui
Owing to their excellent multiplexing ability, high sensitivity, and large dynamic range, immunoassays using surface-enhanced Raman scattering (SERS) as the readout signal have found prosperous applications in fields such as disease diagnosis, environmental surveillance, and food safety supervision. Various ever-increasing demands have promoted SERS-based immunoassays from the classical sandwich-type ones to those integrated with fascinating automatic platforms (e.g., test strips and microfluidic chips). As recent years have witnessed impressive progress in SERS immunoassays, we try to comprehensively cover SERS-based immunoassays from their basic working principles to specific applications...
May 23, 2017: Chemical Reviews
https://www.readbyqxmd.com/read/28534412/exploration-of-biogenic-nano-chemobiotics-fabricated-by-sliver-nanoparticle-and-galactoxyloglucan-with-an-efficient-bio-distribution-in-solid-tumor-investigated-by-sers-fingerprinting
#3
Manu M Joseph, Jyothi B Nair, Ramya N Adukkadan, Neethu Hari, Raveendran Pillai, Ananthakrishnan J Nair, Kaustabh Kumar Maiti, Sreelekha Therakathinal T
An incredible exploration of a dual modality nano composite wherein, chemotherapy in fusion with antibacterial efficacy obtained in a biogenic fabrication, which transformed as a novel nano-chemobiotics (NCB) prevailing fundamental molecular level investigation by surface enhanced Raman scattering (SERS) platform. The nano composite is a facile, robust and eco-friendly constitution between silver nanoparticles (SNPs) and a naturally occurring galactoxyloglucan (PST001) denoted as SNP@PST which displayed biocompatibility with an upgraded selective cytotoxicity towards cancer cells...
May 23, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28533219/obesity-linked-phosphorylation-of-sirt1-by-ck2-inhibits-its-nuclear-localization-and-promotes-fatty-liver
#4
Sung E Choi, Sanghoon Kwon, Sunmi Seok, Zhen Xiao, Kwan-Woo Lee, Yup Kang, Xiaoling Li, Kosaku Shinoda, Shingo Kajimura, Byron Kemper, Jongsook Kim Kemper
Sirtuin1 (SIRT1) deacetylase delays and improves many obesity-related diseases, including non-alcoholic fatty liver disease (NAFLD) and diabetes, and has received great attention as a drug target. SIRT1 function is aberrantly low in obesity, so understanding the underlying mechanisms is important for drug development. Here, we show that obesity-linked phosphorylation of SIRT1 inhibits its function and promotes pathological symptoms of NAFLD. In proteomic analysis, Ser-164 was identified as a major serine phosphorylation site in SIRT1 in obese, but not lean, mice, and this phosphorylation was catalyzed by casein kinase-2 (CK2), the levels of which were dramatically elevated in obesity...
May 22, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/28532802/oral-fluid-versus-blood-sampling-in-group-housed-sows-and-finishing-pigs-feasibility-and-performance-of-antibody-detection-for-porcine-reproductive-and-respiratory-syndrome-virus-prrsv
#5
C Fablet, P Renson, F Pol, V Dorenlor, S Mahé, F Eono, E Eveno, M Le Dimna, D Liegard-Vanhecke, S Eudier, N Rose, O Bourry
The feasibility of using individual and pen-based oral fluid samples to detect PRRSV antibodies in growing-finishing pigs and group-housed sows was investigated. The diagnostic performances of a commercial oral fluid ELISA (OF-ELISA) and a serum ELISA (SER-ELISA) performed on individual or pooled samples from 5 or 10 pigs and sows was evaluated. The performance of the OF-ELISA was also assessed for pen-based oral fluids. Eight hundred and thirty-four pigs and 1598 sows from 42 PRRSV-infected and 3 PRRSV-negative herds were oral fluid sampled and bled...
May 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28532286/hb-bakersfield-hba1-c-151_152insggagcc-the-insertion-of-arg-his-between-codons-49-and-50-of-the-%C3%AE-1-globin-chain-leads-to-increased-oxygen-affinity
#6
Saskia Brunner-Agten, Thomas von Känel, Benno Röthlisberger, Charles Broquet, Andreas R Huber
We describe an insertion variant on the α1-globin gene (HBA1) identified in a 49-year-old woman of Jurassian ancestry presenting with macrocytosis and erythrocytosis. The variant resulted in a peak of 15.5% of the total hemoglobin (Hb) on high performance liquid chromatography (HPLC). Stability and oxygen affinity testing revealed that the variant was stable and had an increased oxygen affinity. Molecular genetic testing detected the heterozygous sequence variant Hb Bakersfield [α50(CE8)His→0; Arg-Ser-His- inserted between 49(CE7) and 51(CE9) of α1; HBA1: c...
January 2017: Hemoglobin
https://www.readbyqxmd.com/read/28530804/characterization-of-growth-patterns-of-nanoscale-organic-films-on-carbon-electrodes-by-surface-enhanced-raman-spectroscopy
#7
Mustafa Supur, Scott R Smith, Richard L McCreery
Electrochemical deposition of aromatic organic molecules by reduction of diazonium reagents enables formation of molecular layers with sufficient integrity for use in molecular electronic junctions of interest to microelectronics. Characterization of organic films with thicknesses in the 1-10 nm range is difficult with Raman spectroscopy, since most molecular structures of electronic interest have Raman cross sections which are too small to observe as either thin films on solid electrodes or within intact molecular junctions...
May 22, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28530726/surface-enhanced-hyper-raman-scattering-sehrs-and-its-applications
#8
REVIEW
Fani Madzharova, Zsuzsanna Heiner, Janina Kneipp
Surface enhanced hyper Raman scattering (SEHRS) is the spontaneous, two-photon excited Raman scattering that occurs for molecules residing in high local optical fields of plasmonic nanostructures. Being regarded as a non-linear analogue of surface enhanced Raman scattering (SERS), SEHRS shares most of its properties, but also has additional characteristics. They include complementary spectroscopic information resulting from different selection rules and a stronger enhancement due to the non-linearity in excitation...
May 22, 2017: Chemical Society Reviews
https://www.readbyqxmd.com/read/28530534/biological-evaluation-of-99mtc-hynic-edda-tricine-ser-3-d4-peptide-for-tumor-targeting
#9
Ziba Kazemi, Mona Haddad Zahmatkesh, Seyed Mohammad Abedi, Seyed Jalal Hosseinimehr
BACKGROUND: D4 small peptide (Leu-Ala-Arg-Leu-Leu-Thr) was selected as an appropriate agent for specific targeting of epidermal growth factor receptor (EGFR). OBJECTIVE: The aim of study was to investigate the 99mTc-labeled D4 peptide for non small cell lung tumor targeting. METHOD: HYNIC-(Ser)3-D4 peptide was labeled with 99mTc using mixture of tricine and ethylenediamine diacetic acid (EDDA) as co-ligands. The in vitro cellular uptake of radiolabebled peptide was evaluated by blocking test on human non small lung cancer (A-549) cell line and its biodistribution was evaluated in A-549 xenografted nude mice...
May 19, 2017: Current Radiopharmaceuticals
https://www.readbyqxmd.com/read/28530395/rapid-detection-of-prosthetic-joint-infection-based-on-magnetically-assisted-surface-enhanced-raman-spectroscopy-ma-sers
#10
Ariana Fargasova, Anna Balzerova, Robert Prucek, Miroslava Htoutou Sedláková, Katerina Bogdanova, Jiri Gallo, Milan Kolar, Vaclav Ranc, Radek Zboril
Accurate and rapid diagnosis of prosthetic joint infection (PJI) is vital for rational and effective therapeutic management of this condition. Several diagnostic strategies have been developed for discriminating between infected and non-infected cases. However, none of them can reliably diagnose the whole spectrum of clinical presentations of PJI. Here we report a new method for PJI detection based on magnetically assisted surface enhanced Raman spectroscopy (MA-SERS) using streptavidin-modified magnetic nanoparticles (MNP@Strep) whose surface is functionalized with suitable biotinylated antibodies and then coated with silver nanoparticles by self-assembly...
May 22, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28528869/pure-membranous-lupus-nephritis-description-of-a-cohort-of-150-patients-and-review-of-the-literature
#11
Lucía Silva-Fernández, Teresa Otón, Anca Askanase, Patricia Carreira, Francisco Javier López-Longo, Alejandro Olivé, Íñigo Rúa-Figueroa, Javier Narváez, Esther Ruiz-Lucea, Mariano Andrés, Enrique Calvo, Francisco Toyos, Juan José Alegre-Sancho, Eva Tomero, Carlos Montilla, Antonio Zea, Esther Uriarte, Jaime Calvo-Alén, Carlos Marras, Víctor M Martínez-Taboada, María Ángeles Belmonte-López, José Rosas, Enrique Raya, Gema Bonilla, Mercedes Freire, José María Pego-Reigosa, Isabel Millán, Adwoa Hughes-Morley
OBJECTIVES: The course and long-term outcome of pure membranous lupus nephritis (MLN) are little understood. The aims of this study are to evaluate the clinical features, course, outcome and prognostic indicators in pure MLN and to determine the impact of ethnicity and the type of health insurance on the course and prognosis of pure MLN. METHODS: We conducted a retrospective review of medical records of 150 patients with pure MLN from Spain and the USA. RESULTS: Mean age was 34...
May 18, 2017: Reumatología Clinica
https://www.readbyqxmd.com/read/28528695/protein-phosphatase-5-promotes-hepatocarcinogenesis-through-interaction-with-amp-activated-protein-kinase
#12
Yao-Li Chen, Man-Hsin Hung, Pei-Yi Chu, Tzu-I Chao, Ming-Hsien Tsai, Li-Ju Chen, Yung-Jen Hsiao, Chih-Ting Shih, Feng-Shu Hsieh, Kuen-Feng Chen
The serine-threonine protein phosphatase family members are known as critical regulators of various cellular functions, such as survival and transformation. Growing evidence suggests that pharmacological manipulation of phosphatase activity exhibits therapeutic benefits. Ser/Thr protein phosphatase 5 (PP5) is known to participate in glucocorticoid receptor (GR) and stress-induced signaling cascades that regulate cell growth and apoptosis, and has been shown to be overexpressed in various human malignant diseases...
May 18, 2017: Biochemical Pharmacology
https://www.readbyqxmd.com/read/28527375/surfactant-enhanced-remediation-of-polycyclic-aromatic-hydrocarbons-a-review
#13
REVIEW
Shanti Lamichhane, K C Bal Krishna, Ranjan Sarukkalige
Polycyclic aromatic hydrocarbons (PAHs) are toxic, mutagenic and carcinogenic organic compounds that are widely present in the environment. The bioremediation of PAHs is an economical and environmentally friendly remediation technique, but it is limited because PAHs have low water solubility and fewer bioavailable properties. The solubility and bioavailability of PAHs can be increased by using surfactants to reduce surface tension and interfacial tension; this method is called surfactant-enhanced remediation (SER)...
May 17, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28526426/the-effects-of-troglitazone-on-ampk-in-hepg2-cells
#14
Katherine M Allen, Kimberly A Coughlan, Fabliha N Mahmood, Rudy J Valentine, Neil B Ruderman, Asish K Saha
AMP-activated protein kinase (AMPK) is an enzyme crucial in cellular metabolism found to be inhibited in many metabolic diseases including type 2 diabetes. Thiazolidinediones (TZDs) are a class of anti-diabetic drug known to activate AMPK through increased phosphorylation at Thr(172), however there has been no research to date on whether they have any effect on inhibition of AMPK's lesser known site of inhibition, Ser(485/491). HepG2 cells were treated with troglitazone and phosphorylation of AMPK was found to increase at both Thr(172) and Ser(485) in a dose- and time-dependent manner...
May 16, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28526415/lnk-deficiency-aggregates-palmitate-induced-preadipocyte-apoptosis
#15
Jie-Yi Du, Chen-Chen Jin, Guo-Hao Wang, Xiong-Qing Huang, Jian-Ding Cheng, Xue-Jun Wen, Xiao-Miao Zhao, Guan-Lei Wang
LNK (SH2B3) is an intracellular adaptor protein that negatively regulates cellular proliferation or self-renewal of hematopoietic stem cells and some other progenitor cells. LNK is also recognized as a key regulator of insulin resistance and inflammatory responses in several tissues and organs. It is unknown the function of LNK in adipose tissue. We previously demonstrated that type 2 diabetes mellitus (T2DM) mouse model had elevated serum free fatty acids (FFAs) levels and increased preadipocyte apoptosis in visceral fat tissue, showing the occurrence of lipotoxicity...
May 16, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28523552/epigenetics-of-huntington-s-disease
#16
Silvia Bassi, Takshashila Tripathi, Alan Monziani, Francesca Di Leva, Marta Biagioli
Huntington's disease (HD) is a genetic, fatal autosomal dominant neurodegenerative disorder typically occurring in midlife with symptoms ranging from chorea, to dementia, to personality disturbances (Philos Trans R Soc Lond Ser B Biol Sci 354:957-961, 1999). HD is inherited in a dominant fashion, and the underlying mutation in all cases is a CAG trinucleotide repeat expansion within exon 1 of the HD gene (Cell 72:971-983, 1993). The expanded CAG repeat, translated into a lengthened glutamine tract at the amino terminus of the huntingtin protein, affects its structural properties and functional activities...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28518223/low-shear-stress-induces-endothelial-reactive-oxygen-species-via-the-at1r-enos-no-pathway
#17
Yuelin Chao, Peng Ye, Linlin Zhu, Xiangquan Kong, Xinliang Qu, Junxia Zhang, Jie Luo, Hongfeng Yang, Shaoliang Chen
Reactive oxygen species (ROS) contribute to many aspects of physiological and pathological cardiovascular processes. However, the underlying mechanism of ROS induction by low shear stress (LSS) remains unclear. Accumulating evidence has shown that the angiotensin II type 1 receptor (AT1R) is involved in inflammation, apoptosis, and ROS production. Our aim was to explore the role of AT1R in LSS-mediated ROS induction. We exposed human umbilical vein endothelial cells (HUVECs) to LSS (3 dyne/cm(2) ) for different periods of time...
May 18, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28516893/design-of-electrohydrodynamic-sprayed-polyethylene-glycol-hydrogel-microspheres-for-cell-encapsulation
#18
Anisa S Qayyum, Era Jain, Grant Kolar, Yonghyun Kim, Scott A Sell, Silviya P Zustiak
Electrohydrodynamic spraying (EHS) has recently gained popularity for microencapsulation of cells for applications in cell delivery and tissue engineering. Some of the polymers compatible with EHS are alginate, chitosan, and other similar natural polymers, which are subject to ionotropic or physical gelation. It is desirable to further extend the use of the EHS technique beyond such polymers for wider biofabrication applications. Here, building upon our previous work of making PEG microspheres via EHS, we utilized the principles of EHS to fabricate cell-laden polyethylene glycol (PEG) hydrogel microspheres...
May 18, 2017: Biofabrication
https://www.readbyqxmd.com/read/28516869/recommendations-for-infectious-disease-screening-in-migrants-to-western-europe-with-inflammatory-arthropathies-before-starting-biologic-agents-results-from-a-multidisciplinary-task-force-of-four-european-societies-sir-ser-simet-semtsi-facing-the-lar
#19
Filippo Bartalesi, Carlo Scirè, Ana Requena-Méndez, Miguel Angel Abad, Dora Buonfrate, Roberto Caporali, Fabrizio Conti, Federico Diaz-Gonzalez, Cruz Fernández-Espartero, Carmen Martinez-Fernandez, Marta Mascarello, Elena Generali, Giovanni Minisola, Aldo Morrone, Jose Muñoz, Patricia Richi, Gariffalia Sakellariou, Joaquin Salas Coronas, Michele Spinicci, Francesco Castelli, Alessandro Bartoloni, Zeno Bisoffi, Francisco Gimenez-Sanchez, Santiago Muñoz-Fernandez, Marco Matucci-Cerinic
OBJECTIVES: Inflammatory arthritis needs infectious disease screening before starting a biologic agent, however, few data are known about migrant patients, who represent a peculiar population which requires a multidisciplinary approach among international health specialists and should also be considered by health authorities. For this reason, the Italian and Spanish Societies of Rheumatology (SIR and SER) and Tropical Medicine (SIMET and SEMTSI) promoted a multidisciplinary task force in order to produce specific recommendations about screening and advices to be considered in migrant patients with inflammatory arthritis candidate to receive biological therapy, according to their geographical origin...
May 8, 2017: Clinical and Experimental Rheumatology
https://www.readbyqxmd.com/read/28513762/water-fluoridation-in-brazilian-cities-at-the-first-decade-of-the-21st-century
#20
Paulo Frazão, Paulo Capel Narvai
OBJECTIVE: To assess the coverage of the fluoridation of the public water supply in Brazilian municipalities at the first decade of the 21st century, according to population size and municipal human development index (MHDI). METHODS: We have used data produced by national information agencies and the United Nations Development Programme. Population size was separated into < 10,000, 10,000-50,000, and > 50,000 inhabitants. The MHDI was classified into < 0...
May 15, 2017: Revista de Saúde Pública
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