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https://www.readbyqxmd.com/read/29330941/hydrophobic-and-hydrophilic-effects-on-water-structuring-and-adhesion-in-denture-adhesives
#1
Simrone K Gill, Nima Roohpour, Yiran An, Julien E Gautrot, Paul D Topham, Brian J Tighe
Denture adhesives are designed to be moisture-sensitive through the inclusion of a blend of polymer salts with varying degrees of water-sensitivity. This enables the adhesive to mix with saliva in vivo and activate its high tack, through the formation of a mucilaginous layer. We report for the first time, the use of differential scanning calorimetry (DSC) to study a series of hydrophobic and hydrophilic polymeric systems in order to correlate water-structuring behavior with adhesion strength. Adhesive bonding of the more hydrophobic variants was higher than that of a commercial-based control and a more hydrophilic polymer system in both lap shear and tensile configurations...
January 13, 2018: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29330459/a-novel-ultra-stable-monomeric-green-fluorescent-protein-for-direct-volumetric-imaging-of-whole-organs-using-clarity
#2
Daniel J Scott, Natalie J Gunn, Kelvin J Yong, Verena C Wimmer, Nicholas A Veldhuis, Leesa M Challis, Mouna Haidar, Steven Petrou, Ross A D Bathgate, Michael D W Griffin
Recent advances in thick tissue clearing are enabling high resolution, volumetric fluorescence imaging of complex cellular networks. Fluorescent proteins (FPs) such as GFP, however, can be inactivated by the denaturing chemicals used to remove lipids in some tissue clearing methods. Here, we solved the crystal structure of a recently engineered ultra-stable GFP (usGFP) and propose that the two stabilising mutations, Q69L and N164Y, act to improve hydrophobic packing in the core of the protein and facilitate hydrogen bonding networks at the surface, respectively...
January 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29330023/unraveling-the-differential-structural-stability-and-dynamics-features-of-t7-endolysin-partially-folded-conformations
#3
Meenakshi Sharma, Dinesh Kumar, Krishna Mohan Poluri
BACKGROUND: Characterization of partially collapsed protein conformations at atomic level is a daunting task due to their inherent flexibility and conformational heterogeneity. T7 bacteriophage endolysin (T7L) is a single-domain amidase that facilitates the lysis of Gram-negative bacteria. T7L exhibits a pH-dependent structural transition from native state to partially folded (PF) conformation. In the pH range 5-3, T7L PF states display differential ANS binding characteristics. METHODS: CD, fluorescence, NMR spectroscopy and lysis assays were used to investigate the structure-stability- dynamics relationships of T7L PF conformations...
January 9, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29329856/effect-of-tyrosinase-aided-crosslinking-on-the-ige-binding-potential-and-conformational-structure-of-shrimp-metapenaeus-ensis-tropomyosin
#4
Ishfaq Ahmed, Liangtao Lv, Hong Lin, Zhenxing Li, Jiaju Ma, Chen Guanzhi, Lirui Sun, Lili Xu
The present study was performed to determine crosslinking and oxidative reactions catalyzed by tyrosinase (Tyr), caffeic acid (CA) and their combination with respect to IgE binding potential and conformational structure of shrimp tropomyosin (TM). Cross-links and IgE binding potentials were analyzed by SDS-PAGE, western blot and indirect ELISA. While structural changes were characterized using surface hydrophobicity, ultraviolet (UV), fluorescence and circular dichroism (CD) spectroscopies. Maximum reduction in the IgG (37...
May 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29329747/antibacterial-properties-of-latarcin-1-derived-cell-penetrating-peptides
#5
Deepthi Poornima Budagavi, Archana Chugh
Cell-penetrating peptides (CPPs) and antimicrobial peptides (AMPs) share certain physicochemical parameters such as amphipathicity, hydrophobicity, cationicity and pI, due to which these two groups of peptides also exhibit overlapping functional characteristics. In our current work, we have evaluated antimicrobial properties of cell-penetrating peptides derived from Latarcin1. Latarcin derived peptide (LDP) exhibited antimicrobial activity against representative microorganisms tested and bactericidal effect against methicillin resistant Staphylococcus aureus (MRSA), which was used as model organism of study in the present work...
January 9, 2018: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29329419/a-single-mutation-unlocks-cascading-exaptations-in-the-origin-of-a-potent-pitviper-neurotoxin
#6
A Carl Whittington, Andrew J Mason, Darin R Rokyta
Evolutionary innovations and complex phenotypes seemingly require an improbable amount of genetic change to evolve. Rattlesnakes display two dramatically different venom phenotypes. Type I venoms are hemorrhagic with low systemic toxicity and high expression of tissue-destroying snake venom metalloproteinases (SVMP). Type II venoms are highly neurotoxic and lack SVMP expression and associated hemorrhagic activity. This dichotomy hinges on Mojave toxin (MTx), a phospholipase A2 (PLA2) based ᵝ-neurotoxin expressed in Type II venoms...
January 10, 2018: Molecular Biology and Evolution
https://www.readbyqxmd.com/read/29329081/presence-of-fe-al-binary-oxide-adsorbent-cake-layer-in-ceramic-membrane-filtration-and-their-impact-for-removal-of-ha-and-bsa
#7
Kyung-Jo Kim, Am Jang
To enhance the removal of natural organic matter (NOM) in ceramic (Ce) membrane filtration, an iron-aluminum binary oxide (FAO) was applied to the ceramic membrane surface as the adsorbent cake layer, and it was compared with heated aluminum oxide (HAO) for the evaluation of the control of NOM. Both the HAO and FAO adsorbent cake layers efficiently removed the NOM regardless of NOM's hydrophobic/hydrophilic characteristics, and the dissolved organic carbon (DOC) removal in NOM for FAO was 1-1.12 times greater than that for HAO, which means FAO was more efficient in the removal of DOC in NOM...
January 5, 2018: Chemosphere
https://www.readbyqxmd.com/read/29329078/schmallenberg-virus-non-structural-protein-nsm-intracellular-distribution-and-role-of-non-hydrophobic-domains
#8
Franziska Kraatz, Kerstin Wernike, Sven Reiche, Andrea Aebischer, Ilona Reimann, Martin Beer
Schmallenberg virus (SBV) induces fetal malformation, abortions and stillbirth in ruminants. While the non-structural protein NSs is a major virulence factor, the biological function of NSm, the second non-structural protein which consists of three hydrophobic transmembrane (I, III, V) and two non-hydrophobic regions (II, IV), is still unknown. Here, a series of NSm mutants displaying deletions of nearly the entire NSm or of the non-hydrophobic domains was generated and the intracellular distribution of NSm was assessed...
January 9, 2018: Virology
https://www.readbyqxmd.com/read/29329012/oxidative-transformation-kinetics-and-pathways-of-albendazole-from-reactions-with-manganese-dioxide
#9
Sin-Yi Liou, Wan-Ru Chen
Albendazole (ABZ) is a benzimidazole-based veterinary anthelmintic used extensively in the treatment of intestinal parasites. Due to its high hydrophobicity, ABZ tends to accumulate in soils and sediments in the environment. This study aims to investigate ABZ's possible degradation by manganese oxides. Minor effects from ionic strength and metal cations on ABZ degradation were observed. By contrast, decrease of pH greatly enhanced the reaction rate. Surface complexation between ABZ and MnO2 was indicated to be the dominant control in the reaction kinetics...
December 29, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/29328994/does-high-pressure-have-any-effect-on-the-structure-of-alpha-amylase-and-its-ability-to-binding-to-the-oligosaccharides-having-3-7-residues-molecular-dynamics-study
#10
S Hooman Vahidi, Mohammad Reza Bozorgmehr, Ali Morsali, S Ali Beyramabadi
Studies have shown that deletion of amino acids from the C-terminus of amylase do not alter its amylolytic activity. Although high pressure is used to modify the structure and function of this enzyme, the effects of high pressures on the structures of the wild-type and truncated amylases have not yet been understood at the molecular level. Using molecular dynamic simulations and docking, we studied the structures of wild-type and truncated Taka-amylases at high pressures (1000-4000 bar). To construct the truncated Taka-amylase, 50 and 100 C-terminal residues were removed in two separate steps...
January 6, 2018: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/29328989/inhibition-of-udp-glucuronosyltransferases-ugts-by-phthalate-monoesters
#11
Zuo Du, Yun-Feng Cao, Sai-Nan Li, Cui-Min Hu, Zhi-Wei Fu, Chun-Ting Huang, Xiao-Yu Sun, Yong-Zhe Liu, Kun Yang, Zhong-Ze Fang
Phthalate monoesters are important metabolites of phthalate esters (PAEs) which have been extensively utilized in industry. This study aims to investigate the inhibition of phthalate monoesters on the activity of various isoforms of UDP-glucuronosyltransferases (UGTs), trying to elucidate the toxicity mechanism of environmental endocrine disruptors from the new perspectives. In vitro recombinant UGTs-catalyzed glucuronidation of 4-methylumbelliferone (4-MU) was employed to evaluate 8 kinds of phthalate monoesters on 11 sorts of main human UGT isoforms...
January 5, 2018: Chemosphere
https://www.readbyqxmd.com/read/29328954/probing-the-stereoselective-interaction-of-ofloxacin-enantiomers-with-corresponding-monoclonal-antibodies-by-multiple-spectrometry
#12
Hongtao Mu, Zhenlin Xu, Yingju Liu, Yuanming Sun, Baoling Wang, Xiulan Sun, Zhanhui Wang, Sergei Eremin, Anatoly V Zherdev, Boris B Dzantiev, Hongtao Lei
Although stereoselective antibody has immense potential in chiral compounds detection and separation, the interaction traits between stereoselective antibody and the corresponding antigenic enantiomers are not yet fully exploited. In this study, the stereospecific interactions between ofloxacin isomers and corresponding monoclonal antibodies (McAb-WR1 and McAb-MS1) were investigated using time-resolved fluorescence, steady-state fluorescence, and circular dichroism (CD) spectroscopic methods. The chiral recognition discrepancies of antibodies with ofloxacin isomers were reflected through binding constant, number of binding sites, driving forces and conformational changes...
January 5, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29328953/the-binding-interaction-of-imazapyr-with-cucurbit-n-uril-n-6-8-combined-experimental-and-molecular-modeling-study
#13
Maali Saad Mokhtar, FakhrEldin O Suliman, Abdalla A Elbashir
The inclusion complexes of imazapyr (IMA) with cucurbit[n]uril, CB[n] (n=6-8), have been investigated. Fluorescence spectroscopy, MALDI-TOF, and 1HNMR were used to investigate and characterize the inclusion complexation of IMA and CB[n] in solutions. Whereas the solid state complexes have been characterized by Fourier transform infrared spectroscopy (FTIR), and powder X-ray diffraction (PXRD). IMA was found to form 1:1 complexes with CB[n] with association constants ranging from 5.80×102-2.65×103. The guest molecule IMA was found to encapsulate into the larger cavities of CB[7] and CB[8], whereas with CB[6] the molecule remains outside the cavity...
January 4, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29328676/stabilizing-leaf-and-branch-compost-cutinase-lcc-with-glycosylation-mechanism-and-effect-on-pet-hydrolysis
#14
Abhijit N Shirke, Christine White, Jacob A Englaender, Allison Sophie Zwarycz, Glenn L Butterfoss, Robert J Linhardt, Richard A Gross
Cutinases are polyester hydrolases that show a remarkable capability to hydrolyze polyethylene terephthalate (PET) to its monomeric units. This revelation has stimulated research aimed at developing sustainable and green cutinase-catalyzed PET recycling methods. Leaf and branch compost cutinase (LCC) is particularly suited towards these ends given its relatively high PET hydrolysis activity and thermostability. Any practical enzymatic PET recycling application will require that the protein have kinetic stability at or above the PET glass transition temperature (Tg, i...
January 12, 2018: Biochemistry
https://www.readbyqxmd.com/read/29328648/two-step-synthesis-and-surface-modification-of-caznos-mn2-phosphors-and-the-fabrication-of-a-luminescent-poly-dimethylsiloxane-film
#15
Zihan Xu, Zhiguo Xia, Quanlin Liu
The CaZnOS:Mn2+ (CZOSM) phosphor has been extensively studied for its excellent optical performance, with a typical red emission band peaking at about 580 nm ascribed to the 4T1(4G)-6A1(6S) transition of Mn2+. Herein the CZOSM phosphor was synthesized by a novel two-step method accompanied by control of the morphology of the precursor in the first step followed by sintering in the second step, which demonstrated improved emission intensity and uniform morphology simultaneously compared to those obtained by the traditional solid-state reaction route...
January 12, 2018: Inorganic Chemistry
https://www.readbyqxmd.com/read/29328626/polystyrene-core-silica-shell-scintillant-nanoparticles-for-low-energy-radionuclide-quantification-in-aqueous-media
#16
Colleen M Janczak, Isen A C Calderon, Zeinab Mokhtari, Craig Alan Aspinwall
β-particle emitting radionuclides are useful molecular labels due to their abundance in biomolecules. Detection of -emission from 3H, 35S, and 33P, important biological isotopes, is challenging due to the low energies (Emax ≤ 300 keV) and short penetration depths (≤ 0.6 mm) in aqueous media. The activity of biologically relevant β-emitters is usually measured in liquid scintillation cocktail (LSC), a mixture of energy-absorbing organic solvents, surfactants, and scintillant fluorophores, which places significant limitations on the ability to acquire time-resolved measurements directly in aqueous biological systems...
January 12, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29327480/membrane-active-peptide-pv3-efficiently-eradicates-multidrug-resistant-pseudomonas-aeruginosa-in-a-mouse-model-of-burn-infection
#17
Hamed Memariani, Delavar Shahbazzadeh, Jean-Marc Sabatier, Kamran Pooshang Bagheri
The aim of this study was to evaluate the topical bactericidal activity of peptide PV3 against a MDR isolate of Pseudomonas aeruginosa in a mouse model of burn infection. The structural analysis of PV3 by circular dichroism spectroscopy indicated a low peptide helical content in water, whereas a high helical content was observed in the presence of the more hydrophobic 50% (v/v) trifluoroethanol/water buffer. A confocal microscopy analysis indicated that the main action of PV3 occurred at the membrane of bacteria...
January 12, 2018: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/29327308/methionine-in-proteins-it-s-not-just-for-protein-initiation-anymore
#18
Jung Mi Lim, Geumsoo Kim, Rodney L Levine
Methionine in proteins is often thought to be a generic hydrophobic residue, functionally replaceable with another hydrophobic residue such as valine or leucine. This is not the case, and the reason is that methionine contains sulfur that confers special properties on methionine. The sulfur can be oxidized, converting methionine to methionine sulfoxide, and ubiquitous methionine sulfoxide reductases can reduce the sulfoxide back to methionine. This redox cycle enables methionine residues to provide a catalytically efficient antioxidant defense by reacting with oxidizing species...
January 11, 2018: Neurochemical Research
https://www.readbyqxmd.com/read/29327016/isoenergic-modification-of-whey-protein-structure-by-denaturation-and-crosslinking-using-transglutaminase
#19
Emil G P Stender, Glykeria Koutina, Kristoffer Almdal, Tue Hassenkam, Alan Mackie, Richard Ipsen, Birte Svensson
Transglutaminase (TG) catalyzes formation of covalent bonds between lysine and glutamine side chains and has applications in manipulation of food structure. Physical properties of a whey protein mixture (SPC) denatured either at elevated pH or by heat-treatment and followed by TG catalyzed crosslinking, have been characterised using dynamic light scattering, size exclusion chromatography, flourescence spectroscopy and atomic force microscopy. The degree of enzymatic crosslinking appeared higher for pH- than for heat-denatured SPC...
January 12, 2018: Food & Function
https://www.readbyqxmd.com/read/29326993/turning-double-hydrophilic-into-amphiphilic-ir825-conjugated-polymeric-nanomicelles-for-near-infrared-fluorescence-imaging-guided-photothermal-cancer-therapy
#20
Guang-Yu Pan, Hao-Ran Jia, Ya-Xuan Zhu, Fu-Gen Wu
Developing biocompatible and photodegradable photothermal agents (PTAs) holds great promise for potential clinical applications in photothermal cancer therapy. Herein, a new PTA was innovatively constructed by conjugating the hydrophobic near-infrared (NIR) heptamethine cyanine molecule IR825-NH2 with a double hydrophilic block copolymer methoxypoly(ethylene glycol)5k-block-poly(l-aspartic acid sodium salt)10 (abbreviated as PEG-PLD) via amine-carboxyl reaction. The as-designed PEG-PLD(IR825) was amphiphilic and could self-assemble into polymeric nanomicelles in aqueous solutions...
January 12, 2018: Nanoscale
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