Pranab K Mukherjee, Quang Tam Nguyen, Jiannan Li, Shuai Zhao, Stephen M Christensen, Gail A West, Jyotsna Chandra, Ilyssa O Gordon, Sinan Lin, Jie Wang, Ren Mao, Douglas Czarnecki, Carla Rayan, Idan Goren, Suhanti Banerjee, Prerna Kotak, Thomas Plesec, Samir Lal, Thomas Fabre, Shoh Asano, Kathryn Bound, Kevin Hart, Chanyoung Park, Robert Martinez, Ken Dower, Thomas A Wynn, Shaomin Hu, Nayden Naydenov, Martin Decaris, Scott Turner, Stefan D Holubar, Scott R Steele, Claudio Fiocchi, Andrei I Ivanov, Kellie M Kravarik, Florian Rieder
BACKGROUND: Fibroblasts play a key role in stricture formation in Crohn's disease (CD) but understanding it's pathogenesis requires a systems-level investigation to uncover new treatment targets. We studied full thickness CD tissues to characterize fibroblast heterogeneity and function by generating the first single cell RNA sequencing (scRNAseq) atlas of strictured bowel and providing proof of principle for therapeutic target validation. METHODS: We performed scRNAseq of 13 fresh full thickness CD resections containing non-involved, inflamed non-strictured, and strictured segments as well as 7 normal non-CD bowel segments...
July 26, 2023: Gastroenterology