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https://www.readbyqxmd.com/read/29778209/ovine-mannheimia-haemolytica-isolates-from-lungs-with-and-without-pneumonic-lesions-belong-to-similar-genotypes
#1
Andrés García-Alvarez, José Francisco Fernández-Garayzábal, Fernando Chaves, Chris Pinto, Dolores Cid
This study investigated the genetic characteristics of 121 ovine Mannheimia haemolytica isolates from lungs with (n = 75) and without pneumonic lesions (n = 46) using multilocus sequence typing (MLST), virulence-associated gene typing and pulsed-field gel electrophoresis (PFGE). Twelve STs were identified with most isolates (81%) belonged to ST16, ST28 and ST8. Analysis of the M. haemolytica MLST Database indicate a wide distribution of these genotypes in small ruminants, never reported in bovine isolates...
June 2018: Veterinary Microbiology
https://www.readbyqxmd.com/read/29778206/occurrence-and-characterisation-of-esbl-encoding-plasmids-among-escherichia-coli-isolates-from-fresh-vegetables
#2
Christin Freitag, G B Michael, Jun Li, Kristina Kadlec, Yang Wang, Melanie Hassel, Stefan Schwarz
Extended-spectrum β-lactamase (ESBL)-producing Escherichia coli isolates have been increasingly reported in different reservoirs. The aims of this study were to investigate the presence of ESBL-producing E. coli in fresh vegetables and to characterise their ESBL gene-carrying plasmids. Among the 245 samples from vegetables investigated during 2011-2013, seven putative ESBL-producing E. coli (salad n = 2, sprouts n = 5) were found. They were subjected to ESBL phenotypic confirmatory tests, detection/sequencing of ESBL genes, antimicrobial susceptibility testing (AST), phylotyping, XbaI-macrorestriction analysis, multilocus sequence typing and transformation...
June 2018: Veterinary Microbiology
https://www.readbyqxmd.com/read/29778191/antimicrobial-susceptibility-and-genetic-relatedness-of-respiratory-tract-pathogens-in-weaner-pigs-over-a-12-month-period
#3
Lisa Niemann, Petra Müller, Jasmin Brauns, Rolf Nathaus, Franziska Schäkel, Kerstin Kipschull, Doris Höltig, Michael Wendt, Stefan Schwarz, Kristina Kadlec
The collaboration project VASIB aims at reducing the antibiotic consumption in pig production by integrating information from consulting expertise in clinical inspection, hygiene, epidemiology, microbiology and pharmacology. In this VASIB subproject, we investigated the antimicrobial susceptibility and relatedness of porcine respiratory tract pathogens. Bordetella bronchiseptica (n = 47), Pasteurella multocida (n = 18) and Streptococcus suis (n = 58) were obtained from weaner pigs at two farms. Antimicrobial susceptibility testing was performed by broth microdilution according to CLSI standards...
June 2018: Veterinary Microbiology
https://www.readbyqxmd.com/read/29774093/distribution-of-virulence-associated-genes-and-antimicrobial-susceptibility-in-clinical-acinetobacter-baumannii-isolates
#4
Chao Liu, Yaowen Chang, Ying Xu, Yun Luo, Linrong Wu, Zhanjun Mei, Shigang Li, Rui Wang, Xu Jia
Acinetobacter baumannii is undoubtedly one of the most clinically significant pathogens. The multidrug resistance and virulence potential of A. baumannii are responsible for hospital-acquired nosocomial infections. Unlike numerous investigations on the drug-resistant epidemiology of A. baumanni , virulence molecular epidemiology is less studied. Here, we collected 88 A. baumannii clinical isolates, tested their antimicrobial susceptibility to 10 commonly used antibiotics and analyzed the distribution of 9 selected virulence-associated genes, aims to investigate the primary characteristics of the virulence-associated genes that exist in clinically multidrug resistant (MDR) and non-MDR isolates of A...
April 24, 2018: Oncotarget
https://www.readbyqxmd.com/read/29763630/clinical-and-molecular-epidemiologic-characteristics-of-carbapenem-resistant-klebsiella-pneumoniae-infection-colonization-among-neonates-in-china
#5
Dandan Yin, Lei Zhang, Aimin Wang, Leiyan He, Yun Cao, Fupin Hu, Chuanqing Wang
BACKGROUND: The spread of carbapenem-resistant Klebsiella pneumoniae (CR-KPN) is a major concern, but data on CR-KPN infection/colonization in paediatric populations are limited. AIM: This study aimed to analyze epidemiologic and clinical characteristics, therapeutic options of CR-KPN infections in neonates in China. METHODS: A retrospective study was performed at the Children's Hospital of Fudan University, including 88 neonates with CR-KPN admitted between November 2015 and October 2016...
May 12, 2018: Journal of Hospital Infection
https://www.readbyqxmd.com/read/29753135/daptomycin-and-vancomycin-heteroresistance-revealed-among-cc5-sccmecii-mrsa-clone-and-in-vitro-evaluation-of-treatment-alternatives
#6
Jessica Baleiro Okado, Juliana Sposto Avaca-Crusca, Alexandre Lima Oliveira, Andrei Nicoli Gebieluca Dabul, Ilana Lopes Baratella da Cunha Camargo
INTRODUCTION: Methicillin-resistant Staphylococcus aureus (MRSA) is a threat to clinical treatment. Besides high resistance, presence of heterogeneous vancomycin intermediate S. aureus (hVISA) and heterogeneous daptomycin non-susceptible S. aureus (hDNSSA) in the hospital environment are underestimated and associated with treatment failure. OBJECTIVES: To investigate a MRSA dissemination in a Brazilian hospital and to evaluate the efficacy of some treatment options in vitro...
May 9, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29753133/unraveling-the-mechanisms-of-nitrofurantoin-resistance-and-epidemiological-characteristics-among-escherichia-coli-clinical-isolates
#7
Xiaoxiao Zhang, Yizhi Zhang, Fang Wang, Chong Wang, Lijiang Chen, Haiyang Liu, Hong Lu, Hong Wen, Tieli Zhou
To investigate the mechanisms of nitrofurantoin resistance and epidemiological characteristics in E. coli clinical isolates, 18 (1.2%, 18/1444) nitrofurantoin-resistant E. coli strains were identified from a total of 1444 E. coli clinical strains collected from our hospital. The present study indicated that the minimum inhibitory concentration of nitrofurantoin to nitrofurantoin-resistant strains were in a range of 128 - ≥512 μg /ml. The prevalence of nfsA gene in resistant, intermediate and sensitive strains were 88...
May 9, 2018: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29750597/dissemination-of-vana-type-enterococcus-faecium-isolates-in-hungary
#8
Szilvia Melegh, Adrienn Nyul, Krisztina Kovács, Tamás Kovács, Ágoston Ghidán, Zsuzsanna Dombrádi, Judit Szabó, Brigitta Berta, Virág Lesinszki, Judit Pászti, Ákos Tóth, Gyula Mestyán
Although vanA carrying Enterococcus faecium human clinical isolates have been rarely found in Hungary before 2012, they have been detected in continuously increasing numbers since then. To identify factors associated with their dissemination, we investigated the clonal relatedness and plasmids of 30 vanA carrying E. faecium isolates originating from different Hungarian healthcare institutions from 2012 to 2014. Molecular typing of the isolates (n = 30) was performed with pulsed-field gel electrophoresis (PFGE), multilocus sequence typing, Tn1546 polymerase chain reaction mapping, plasmid restriction fragment length polymorphism analysis, and sequencing...
May 11, 2018: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29747038/emerging-high-level-ciprofloxacin-resistance-and-molecular-basis-of-resistance-in-salmonella-enterica-from-humans-food-and-animals
#9
Dai Kuang, Jianmin Zhang, Xuebin Xu, Weimin Shi, Sheng Chen, Xiaowei Yang, Xudong Su, Xianming Shi, Jianghong Meng
Disease caused by nontyphoidal serotypes of Salmonella enterica is the leading cause of foodborne illness worldwide. Many serotypes have also developed resistance to antimicrobials commonly used for the treatment of salmonellosis. Here we analyze 179 ciprofloxacin-resistant isolates identified among 3680 Salmonella isolated from humans, food, animals, and water collected in Shanghai, China. They were assessed for antimicrobial susceptibility, plasmid-mediated quinolone resistance (PMQR) determinants, and mutations in quinolone resistance determination regions (QRDRs); genetic relatedness was examined using PFGE...
May 2, 2018: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29744322/dissemination-of-multidrug-resistant-class-i-and-ii-integrons-and-molecular-typing-of-ctx-m-producing-klebsiella-pneumoniae
#10
Alisha Akya, Azam Elahi, Roya Chegenelorestani, Mahya Rezaee
Introduction: Klebsiella pneumoniae ( K. pneumoniae ) is an important opportunistic pathogen causes serious community and hospital-acquired infections, which is highly resistant to antibiotics. We aimed to determine the frequency of multidrug resistant (MDR) and molecular typing of clinical isolates of K. pneumoniae . Methodology: One hundred isolates of K. pneumoniae were collected from clinical samples in three general hospitals in Kermanshah. The antimicrobial susceptibility and extended-spectrum beta-lactamases (ESBL) production of isolates were determined using disk diffusion and combined disk methods, respectively...
April 2018: International Journal of Applied and Basic Medical Research
https://www.readbyqxmd.com/read/29741928/comparative-study-on-antibiotic-resistance-and-dna-profiles-of-salmonella-enterica-serovar-typhimurium-isolated-from-humans-retail-foods-and-the-environment-in-shanghai-china
#11
Zengfeng Zhang, Chenyang Cao, Bin Liu, Xuebin Xu, Yanfei Yan, Shenghui Cui, Sheng Chen, Jianghong Meng, Baowei Yang
We characterized antibiotic resistance profiles, antibiotic resistance-associated genes, and pulsed-field gel electrophoresis (PFGE) patterns of 145 Salmonella enterica serotype Typhimurium isolates from human infections and retail foods that were possibly responsible for salmonellosis outbreaks from 2008 to 2012 in Shanghai, China. Resistance to at least three antibiotics was found in 66.7% of chicken isolates, 76.5% of duck isolates, 77.8% of pork isolates, and 80.5% of human isolates. Seven antibiotic resistance phenotypes were detected in chicken isolates, 16 in pork isolates, 17 in duck isolates, and 50 in human isolates...
May 9, 2018: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/29735381/escherichia-coli-isolates-from-patients-with-inflammatory-bowel-disease-expec-virulence-and-colicin-determinants-are-more-frequent-compared-to-healthy-controls
#12
Lenka Micenková, Lucia Frankovičová, Iva Jaborníková, Juraj Bosák, Petr Dítě, Jan Šmarda, Martin Vrba, Alena Ševčíková, Marta Kmeťová, David Šmajs
A set of 178 Escherichia coli isolates taken from patients with inflammatory bowel disease (IBD) was analyzed for bacteriocin production and tested for the prevalence of 30 bacteriocin and 22 virulence factor determinants. Additionally, E. coli phylogenetic groups were also determined. Pulsed-field gel electrophoresis (PFGE) was used for exclusion of clonal character of isolates. Results were compared to data from a previously published analysis of 1283 fecal commensal E. coli isolates. The frequency of bacteriocinogenic isolates (66...
May 1, 2018: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/29734665/pediatric-methicillin-resistant-staphylococcus-aureus-osteoarticular-infections
#13
REVIEW
Ashlesha Kaushik, Helen Kest
Osteoarticular infections (OSI) are a significant cause of hospitalizations and morbidity in young children. The pediatric patient with OSI presents unique challenges in diagnosis and management due to higher morbidity, effect on growth plate with associated long-lasting sequelae, and challenges in early identification and management. Methicillin-resistant Staphylococcus aureus (MRSA), first described in the 1960s, has evolved rapidly to emerge as a predominant cause of OSI in children, and therefore empiric treatment for OSI should include an antibiotic effective against MRSA...
May 4, 2018: Microorganisms
https://www.readbyqxmd.com/read/29732329/-salmonella-brandenburg-in-the-pork-chain-in-italy-genetic-comparison-with-the-human-isolates
#14
Silvia Bonardi, Marina Morganti, Giovanni Pupillo, Franco Brindani
Salmonella Brandenburg ranked 16th among the serovars responsible for human infections in EU in 2015 and it was found to be associated with swine. In Emilia- Romagna and Lombardy regions of northern Italy, S. Brandenburg was isolated from mesenteric lymph nodes, fecal matter, carcasses and conveyor belts at pig slaughterhouses in 2014 and 2015. In the same area, S. Brandenburg was detected in pork salami in 2015. In the present study, 12 isolates of S. Brandenburg recovered from the pork food-chain were typed by Xba I PFGE and their three profiles were compared to all human S ...
March 31, 2018: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/29728111/emergence-of-multidrug-resistant-providencia-rettgeri-isolates-co-producing-ndm-1-carbapenemase-and-per-1-extended-spectrum-%C3%AE-lactamase-causing-a-first-outbreak-in-korea
#15
Saeam Shin, Seok Hoon Jeong, Hyukmin Lee, Jun Sung Hong, Min-Jeong Park, Wonkeun Song
BACKGROUND: Nosocomial outbreak due to carbapenem-resistant Enterobacteriaceae has become serious challenge to patient treatment and infection control. We describe an outbreak due to a multidrug-resistant Providencia rettgeri from January 2016 to January 2017 at a University Hospital in Seoul, Korea. METHODS: A total of eight non-duplicate P. rettgeri isolates were discovered from urine samples from eight patients having a urinary catheter and admitted in a surgical intensive care unit...
May 5, 2018: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/29727717/coexistence-of-ndm-1-producing-escherichia-coli-and-citrobacter-freundii-in-the-same-patient
#16
Bo Zhu, Chaoqun Ying, Hao Xu, Jingjing Ying
OBJECTIVES: Although blaNDM-1 has now been widely detected in various Enterobacteriaceae clinical isolates, multiple blaNDM-1 colonizations within the same patient remain rare. The aim of the study was to describe a patient with respiratory tract colonization of NDM-1-producing Escherichia coli and Citrobacter freundii during a hospitalization in China. METHODS: Two carbapenem-resistant isolates were analysed. Susceptibility testing, metallo-β-lactamase production, conjugation, plasmid analysis, and molecular typing were performed...
May 1, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29727511/effect-of-electron-beam-and-gamma-radiation-on-drug-susceptible-and-drug-resitant-listeria-monocytogenes-strains-in-salmon-under-different-temperature
#17
Krzysztof Skowron, Katarzyna Grudlewska, Grzegorz Gryń, Karolina Jadwiga Skowron, Agnieszka Świeca, Zbigniew Paluszak, Zbigniew Zimek, Andrzej Rafalski, Eugenia Gospodarek-Komkowska
AIMS: To investigate the effect of gamma radiation and high energy electron beam doses on the inactivation of antibiotic-susceptible and antibiotic-resistant Listeria monocytogenes strains inoculated on the surface of raw salmon fillets stored at different temperature (-20°C, 4°C and 25°C). METHODS AND RESULTS: The population of bacteria strains resistance to penicillin, ampicillin, meropenem, erythromycin and trimethoprim-sulfamethoxazole was generated. When using gamma irradiation, the theoretical lethal dose ranged from 1...
May 4, 2018: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29724270/enterotoxin-gene-profile-of-methicillin-resistant-staphylococcus-pseudintermedius-isolates-from-dogs-humans-and-the-environment
#18
Nathita Phumthanakorn, Punpichaya Fungwithaya, Pattrarat Chanchaithong, Nuvee Prapasarakul
PURPOSE: This study aimed to detect and identify staphylococcal enterotoxin (SE) genes in methicillin-resistant Staphylococcus pseudintermedius (MRSP) strains from different sources, and to investigate the relationship between their sequence types (STs) and SE gene patterns. METHODOLOGY: The profiles of 17 SE genes in 93 MRSP strains isolated from dogs (n=43), humans (n=18) and the environment (n=32) were detected by PCR. Multilocus sequence typing (MLST), SCCmec typing and pulsed-field gel electrophoresis (PFGE) were used to analyse the clonal relatedness between the molecular type and SE gene profiles...
May 4, 2018: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29713466/dissemination-of-bla-ndm-5-gene-via-an-incx3-type-plasmid-among-non-clonal-escherichia-coli-in-china
#19
Xi Li, Ying Fu, Mengyuan Shen, Danyan Huang, Xiaoxing Du, Qingfeng Hu, Yonglie Zhou, Dairong Wang, Yunsong Yu
Background: The emergence and spread of New Delhi metallo-β-lactamase-producing Enterobacteriaceae has been a serious challenge to manage in the clinic due to its rapid dissemination of multi-drug resistance worldwide. As one main type of carbapenemases, New Delhi metallo-β-lactamase (NDM)is able to confer resistance to almost all β-lactams, including carbapenems, in Enterobacteriaceae . Recently, New Delhi metallo-β-lactamase-5 attracted extensive attention because of increased resistance to carbapenems and widespread dissemination...
2018: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/29709977/report-of-relapse-typhoid-fever-cases-from-kolkata-india-recrudescence-or-reinfection
#20
Sriparna Samajpati, Surojit Das, Ujjwayini Ray, Shanta Dutta
Three relapse cases out of 107 hospital attending typhoid cases within a period of 2 years (2014-2016) from Apollo Geneagles hospital, Kolkata, India were reported. Of three cases, two were treated with ceftriaxone for 7 days and one was for 14 days in the first episode of typhoid fever. Six Salmonella Typhi (S. Typhi) isolates, obtained from three patients in both the episodes, were subjected to antimicrobial susceptibility test (AST), detection of QRDR mutation and molecular subtyping by PFGE, MLVA, MLST, CRISPR and H58 haplotyping...
April 27, 2018: Japanese Journal of Infectious Diseases
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