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https://www.readbyqxmd.com/read/28550058/whole-genome-and-core-genome-multilocus-sequence-typing-and-single-nucleotide-polymorphism-analyses-of-listeria-monocytogenes-associated-with-an-outbreak-linked-to-cheese-united-states-2013
#1
Yi Chen, Yan Luo, Heather Carleton, Ruth Timme, David Melka, Tim Muruvanda, Charles Wang, George Kastanis, Lee S Katz, Lauren Turner, Angela Fritzinger, Terence Moore, Robert Stones, Joseph Blankenship, Monique Salter, Mickey Parish, Thomas S Hammack, Peter S Evans, Cheryl L Tarr, Marc W Allard, Errol A Strain, Eric W Brown
Epidemiological findings of a listeriosis outbreak in 2013 implicated Hispanic-style cheese produced by Company A, and pulsed-field gel electrophoresis (PFGE) and whole genome sequencing (WGS) were performed on clinical isolates and representative isolates collected from Company A cheese and environmental samples during the investigation. The results strengthened the evidence for cheese as the vehicle. Surveillance sampling and WGS three months later revealed that the equipment purchased by Company B from Company A yielded an environmental isolate highly similar to all outbreak isolates...
May 26, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28549878/outbreak-of-imipenem-resistant-acinetobacter-baumannii-in-different-wards-at-a-regional-hospital-related-to-untrained-bedside-caregivers
#2
Ching-Hsun Wang, Jin-Feng Li, Li-Yueh Huang, Fu-Mei Lin, Ya-Sung Yang, L Kristopher Siu, Feng-Yee Chang, Jung-Chung Lin
BACKGROUND: This study describes an outbreak caused by imipenem-resistant Acinetobacter baumannii (IRAB) involving 2 general wards at the Penghu branch of Tri-Service General Hospital. METHODS: Clinical data obtained from the patients with IRAB during an outbreak from May 2014-October 2014 were reviewed. Microbiologic sampling from the environment and the hands of health care workers (HCWs) was performed. Clinical isolates from case patients were genotyped using pulsed-field gel electrophoresis (PFGE)...
May 23, 2017: American Journal of Infection Control
https://www.readbyqxmd.com/read/28545990/retrospective-survey-of-mcr-1-and-mcr-2-in-german-pig-fattening-farms-2011-2012
#3
Nicole Roschanski, Linda Falgenhauer, Mirjam Grobbel, Sebastian Guenther, Lothar Kreienbrock, Can Imirzalioglu, Uwe Roesler
In November 2015, the first plasmid-encoded colistin resistance gene, mcr-1, was described in animals and in humans in China. Subsequently, a multitude of further studies was performed and quite recently the global spread of mcr-1 as well as the occurrence of a new gene variant, mcr-2, was reported. To obtain an overview of the occurrence of the colistin resistance genes mcr-1 and mcr-2 in German pig farms, a retrospective study, including 436 boot swab and pooled faeces samples collected from 58 pig-fattening farms throughout Germany, was performed...
May 22, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28542807/genetic-diversity-virulotyping-and-antimicrobial-resistance-susceptibility-of-yersinia-enterocolitica-isolated-from-pigs-and-porcine-products-in-malaysia
#4
Kwai Lin Thong, Lai Kuan Tan, Peck Toung Ooi
BACKGROUND: The objectives of this study were to determine the antimicrobial resistance, virulotypes and genetic diversity of Yersinia enterocolitica isolated from uncooked porcine food and live pigs in Malaysia. RESULTS: Thirty-two non-repeat Y. enterocolitica strains of three bioserotypes (3 variant/O:3, n=27; 1B/O:8, n=3; 1A/O:5, n=2) were analysed. About 90% of strains were multidrug-resistant with MAR index <0.2 and majority of the strains were resistant to nalidixic acid, clindamycin, ampicillin, ticarcillin, tetracycline and amoxicillin...
May 24, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28539344/investigation-of-kingella-kingae-invasive-infections-outbreaks-in-day-care-facilities-assessment-of-a-rapid-genotyping-tool-targeting-the-dna-uptake-sequence
#5
Philippe Bidet, Violaine Tran Quang, Pablo Yagusky, André Birgy, Stéphane Bonacorsi, Romain Basmaci
Objectives Outbreaks of Kingella kingae invasive infections have recently been reported in day-care centers. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) revealed that although the invasive strains had a widespread dissemination in the day-care population, less virulent strains were also found to be circulating in the facility. However, these typing tools are costly, time-consuming and labor-intensive, and provide delayed results. A study was conducted to assess the performance of a rapid and cost-effective genotyping tool targeting the DNA uptake sequence (DUS) in the investigation of outbreaks of K...
May 24, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28536672/response-to-bile-salts-in-clinical-strains-of-acinetobacter-baumannii-lacking-the-adeabc-efflux-pump-virulence-associated-with-quorum-sensing
#6
Maria López, Lucia Blasco, Eva Gato, Astrid Perez, Laura Fernández-Garcia, Luis Martínez-Martinez, Felipe Fernández-Cuenca, Jesús Rodríguez-Baño, Alvaro Pascual, German Bou, Maria Tomás
Introduction:Acinetobacter baumannii is an opportunistic nosocomial pathogen associated with multiple infections. This pathogen usually colonizes (first stage of microbial infection) host tissues that are in contact with the external environment. As one of the sites of entry in human hosts is the gastrointestinal tract, the pathogen must be capable of tolerating bile salts. However, studies analyzing the molecular characteristics involved in the response to bile salts in clinical strains of A. baumannii are scarce...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28535790/clonal-replacement-of-epidemic-kpc-producing-klebsiella-pneumoniae-in-a-hospital-in-china
#7
Yuying Liang, Xiuyun Yin, Lijun Zeng, Shuiping Chen
BACKGROUND: Klebsiella pneumoniae is a frequent nosocomial pathogen causing difficult-to-treat infections worldwide. The prevalence of Klebsiella pneumoniae carbapenemase (KPC)-producing Klebsiella pneumoniae (KPC-KP) is increasing in China. The aim of this study was to investigate the molecular epidemiology of KPC-KP in a nosocomial outbreak. METHODS: Fifty-four KPC-KP isolates were consecutively collected between November 2013 and August 2014 during a KPC-KP outbreak in a tertiary care hospital in Beijing, China...
May 23, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28532801/dissemination-of-erm-b-and-its-associated-multidrug-resistance-genomic-islands-in-campylobacter-from-2013-to-2015
#8
Dejun Liu, Fengru Deng, Yanan Gao, Hong Yao, Zhangqi Shen, Congming Wu, Yang Wang, Jianzhong Shen
A total of 1372 Campylobacter isolates (1107 Campylobacter coli and 265 Campylobacter jejuni) were obtained from 3462 samples collected from slaughterhouses and farms in three representative regions of China (Shandong, Guangdong, and Shanghai) over three successive years (2013-2015). Of these, 84 (84/1372, 6.1%) were erm(B)-positive, and all 84 positive isolates were identified as C. coli (83 chicken isolates and one swine isolate). The prevalence of erm(B) in Campylobacter isolates was compared amongst the different regions and between the three years investigated...
May 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28532797/genetic-basis-for-loss-of-immuno-reactive-o-chain-in-salmonella-enterica-serovar-enteritidis-veterinary-isolates
#9
Istvan Szabo, Marianne Grafe, Nicole Kemper, Ernst Junker, Burkhard Malorny
Fifty-two rough Salmonella enterica serovar Enteritidis (S. Enteritidis) isolates from broilers and the environment were characterized for their serological and genotypic properties. Under routine diagnostic serotyping methods such isolates lack the immuno-reactivity of the O-chain of the lipopolysaccharide (LPS), and are referred to as non-typeable. Using a modified slide agglutination method, the isolates could be differentiated into three different serological variants. Twenty-six isolates (50%) were defined as semi-rough, nineteen isolates (37%) as deep-rough, four isolates (8%) as rough and three isolates could not be assigned...
May 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28532793/streptococcus-agalactiae-in-elephants-a-comparative-study-with-isolates-from-human-and-zoo-animal-and-livestock-origin
#10
Tobias Eisenberg, Jörg Rau, Uta Westerhüs, Tobias Knauf-Witzens, Ahmad Fawzy, Karen Schlez, Michael Zschöck, Ellen Prenger-Berninghoff, Carsten Heydel, Reinhard Sting, Stefanie P Glaeser, Dipen Pulami, Mark van der Linden, Christa Ewers
Streptococcus (S.) agalactiae represents a significant pathogen for humans and animals. However, there are only a few elderly reports on S. agalactiae infections in wild and zoo elephants even though this pathogen has been isolated comparatively frequently in these endangered animal species. Consequently, between 2004 and 2015, we collected S. agalactiae isolates from African and Asian elephants (n=23) living in four different zoos in Germany. These isolates were characterised and compared with isolates from other animal species (n=20 isolates) and humans (n=3)...
May 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28515641/molecular-typing-and-antimicrobial-susceptibility-of-staphylococcus-aureus-strains-isolated-from-raw-milk-cheese-minced-meat-and-chicken-meat-samples
#11
Hayriye Yeşim Can, Mehmet Elmalı, Alper Karagöz
The objectives of this study were: i) to detect the presence of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in raw milk, cheese, beef minced meat, and chicken meat samples; ii) to evaluate the antimicrobial susceptibility of the isolates; and iii) to determine clonal relation among the isolates by using pulsed-field gel electrophoresis (PFGE) method. Therefore, a total of 160 food samples were randomly collected between August 2014 and May 2015 in Hatay province, located in the southern Turkey...
2017: Korean Journal for Food Science of Animal Resources
https://www.readbyqxmd.com/read/28513778/phenotypic-and-genotypic-characterization-of-salmonella-spp-isolated-from-foods-and-clinical-samples-in-brazil
#12
Andrea L Miranda, Soraia M Cordeiro, Joice N Reis, Lucas G Cardoso, Alaíse G Guimarães
Increasing antimicrobial resistance in Salmonella species has been a serious problem for public health worldwide. This study examines Salmonella spp. recovered from foods and clinical samples on serotype, antimicrobial resistance and PFGE genotypes. It identified 91 salmonellae, belonging to 31 different serotypes, from 36 isolates from food and 55 clinical samples. Salmonella Infantis (16.5%) and Salmonella Enteritidis (13.7%) are the most common among food isolates, whereas Salmonella Enteritidis (29.0%) and Salmonella Typhimurium (16...
May 15, 2017: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/28512290/evolution-of-drug-resistant-acinetobacter-baumannii-after-dcd-renal-transplantation
#13
Hong Jiang, Luxi Cao, Lihui Qu, Tingting Qu, Guangjun Liu, Rending Wang, Bingjue Li, Yuchen Wang, Chaoqun Ying, Miao Chen, Yingying Lu, Shi Feng, Yonghong Xiao, Junwen Wang, Jianyong Wu, Jianghua Chen
Infection after renal transplantation remains a major cause of morbidity and death, especially infection from the extensively drug-resistant bacteria, A. baumannii. A total of fourteen A. baumannii isolates were isolated from the donors' preserved fluid from DCD (donation after cardiac death) renal transplantation and four isolates in the recipients' draining liquid at the Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, from March 2013 to November 2014. An outbreak of A...
May 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28509842/molecular-epidemiology-of-methicillin-resistant-and-methicillin-susceptible-staphylococcus-aureus-in-the-ovine-dairy-chain-and-in-farm-related-humans
#14
Guerrino Macori, Giuseppina Giacinti, Alberto Bellio, Silvia Gallina, Daniela Manila Bianchi, Daniele Sagrafoli, Nicla Marri, Gilberto Giangolini, Simonetta Amatiste, Lucia Decastelli
Staphylococcus aureus is a major cause of clinical infections in humans and its enterotoxins cause foodborne disease. In the present study, we tested a total of 51 isolates of S. aureus from small-ruminant dairy farms with artisan dairy facilities, all located in Latium, Italy. The farms have a known history of a high prevalence of methicillin-resistant S. aureus (MRSA). Most of the MRSA isolates (27 of 51) belonged to spa-type t127 (43.1%), followed by t2678 (3.9%), t044 (2%), t1166 (2%), and t1773 (2%). PFGE performed on mecA positive strains identified one cluster (≥ 80% of similarity), comprising 22 MRSA...
May 16, 2017: Toxins
https://www.readbyqxmd.com/read/28506660/specific-clones-of-staphylococcus-lugdunensis-may-be-associated-with-colon-carcinoma
#15
Norihisa Noguchi, Mari Fukuzawa, Takeaki Wajima, Kaoru Yokose, Masae Suzuki, Hidemasa Nakaminami, Takashi Kawai, Fuminori Moriyasu, Masanori Sasatsu
Staphylococcus lugdunensis produces a tannase with activity that may be associated with the onset of colon carcinoma. To clarify this feature of colon carcinoma-associated S. lugdunensis, we obtained isolates from healthy subjects and patients with colon adenomas and carcinomas and analyzed their genetic backgrounds. In total, 40 S. lugdunensis isolates from 288 rectal swabs collected between 2002 and 2008 were used. These isolates were classified into four groups according to the diseases of the subjects: healthy (n=13), colon carcinoma (n=13), colon adenoma (n=9), and unknown (n=5)...
May 11, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28505330/acinetobacter-baumannii-transfers-the-blandm-1-gene-via-outer-membrane-vesicles
#16
Somdatta Chatterjee, Ayan Mondal, Shravani Mitra, Sulagna Basu
Objectives: To investigate the transmission of the gene encoding New Delhi metallo-β-lactamase-1 ( bla NDM-1 ) through outer membrane vesicles (OMVs) released from an Acinetobacter baumannii strain (A_115). Methods: Isolation and purification of OMVs by density gradient from a carbapenem-resistant clinical strain of A. baumannii harbouring plasmid-mediated bla NDM-1 and aac(6')-Ib-cr genes was performed. DNA was purified from the OMVs and used for PCR and dot-blot analysis...
May 12, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28504096/multiple-clones-and-low-antimicrobial-resistance-rates-for-salmonella-enterica-serovar-infantis-populations-in-greece
#17
Theofilos Papadopoulos, Evanthia Petridou, Antonios Zdragas, Georgia Mandilara, Georgios Vafeas, Maria Passiotou, Alkiviadis Vatopoulos
All the Salmonella enterica ser. Infantis strains isolated under official control programs in Greece during a four year period were studied, 23 of human origin, 16 from food animals and one from food. Molecular analyses (PFGE) in combination with antimicrobial susceptibility testing were used to study whether the occurrence S. Infantis in Greece resulted from different biotypes or a successful spread of one clone. Low rates of antimicrobial resistance were observed, except for streptomycin among human isolates (48%), indicating that selective pressure due to consumption of antimicrobials has not resulted the spread of dominant clones...
April 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/28497996/characterization-of-the-salmonella-enterica-serotype-isangi-isolated-from-patients-for-the-first-time-in-china
#18
Xin-Peng Li, Ri-Hong Gao, Pei-Bin Hou, Yan-Yan Ren, Hua-Ning Zhang, Kui-Ying Jiang, Yu-Zhen Chen, Zi-Gang Qi, Min Xu, Zhen-Wang Bi
No studies have reported the isolation of serotype Salmonella Isangi from cases of salmonellosis in mainland China. We investigated an outbreak of foodborne disease with salmonella and collected the samples from the patients and surplus foods. Salmonella strains were isolated and the serotype was identified according to the Kauffmann-White scheme. The relatedness of the isolates was determined using pulsed-field gel electrophoresis (PFGE) and whole genome sequencing (WGS). Antimicrobial susceptibility was conducted by the broth microdilution method...
May 12, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/28490008/an-uncommon-st1224-ndm-1-producing-klebsiella-pneumoniae-isolated-from-the-bloodstream-of-a-leukemia-patient-in-china
#19
Xiaoxiao Zhang, Jia Du, Cui Zhou, Jianming Cao, Hong Lu, Haiyang Liu, Wenzi Bi, Tieli Zhou
AIMS: This study aimed to analyze the clinical data and characteristics of an NDM-1 (New Delhi metallo-β-lactamase-1)-producing Klebsiella pneumoniae isolated from the bloodstream of a leukemia patient. MATERIALS AND METHODS: A retrospective analysis was used for the clinical data of the patient. The modified Hodge test (MHT) and ethylenediaminetetraacetic acid (EDTA)-disk synergy test were used for detecting metallo-β-lactamase. Antibiotic resistance was determined using the agar dilution method...
May 11, 2017: Chemotherapy
https://www.readbyqxmd.com/read/28485710/epidemiological-typing-of-multidrug-resistant-klebsiella-pneumoniae-which-causes-paediatric-ventilator-associated-pneumonia-in-egypt
#20
Eman R Mohamed, Sherine A Aly, Hamada M Halby, Shabaan H Ahmed, Amira M Zakaria, Osama M El-Asheer
PURPOSE: Multidrug-resistant Klebsiella pneumoniae is a common nosocomial pathogen that plays an important role in ventilator-associated pneumonia (VAP). This study aimed to define the clonal relatedness of K. pneumoniae strains isolated from paediatric VAP in addition to those isolated from environmental samples. METHODOLOGY: This study included 19 clinical and 4 environmental K. pneumoniae isolates recovered from the paediatric intensive care unit (PICU) in Assiut University Children's Hospital...
May 2017: Journal of Medical Microbiology
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