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Xinfeng Zhang, Lei Wang, Renpeng Li, Peiqiang Hou, Yuanqing Zhang, Ming Fang, Bin Hu
The objective of this study was to investigate the presence and characterization of Klebsiella pneumoniae (KP) from the intestinal tract. 43 KP strains were isolated from 551 stool specimens from diarrhea patients from 2013 to 2016,with a carriage rate of 7.80%. All isolates were hypervirulent KP (hvKp) strains with hypermucoviscosity (HM) phenotype. The virulence factors and antibiotic resistance exhibited diverse distribution. 2.33% (1/43), 2.33% (1/43), 6.98% (3/43), 6.98% (3/43), 4.65% (2/43), 11.63% (5/43) and 18...
March 14, 2018: Letters in Applied Microbiology
Cristiana Maurella, Silvia Gallina, Giuseppe Ru, Daniela Adriano, Alberto Bellio, Daniela Manila Bianchi, Laura Chiavacci, Maria Ines Crescio, Margherita Croce, Valeria D'Errico, Maria Franca Dupont, Alessandro Marra, Ubaldo Natangelo, Francesco Pomilio, Angelo Romano, Stefano Stanzione, Teresa Zaccaria, Fabio Zuccon, Maria Caramelli, Lucia Decastelli
In May 2016, two separate clusters of febrile gastroenteritis caused by Listeria monocytogenes were detected by the local health authority in Piedmont, in northern Italy. We carried out epidemiological, microbiological and traceback investigations to identify the source. The people affected were students and staff members from two different schools in two different villages located in the Province of Turin; five of them were hospitalised. The epidemiological investigation identified a cooked beef ham served at the school canteens as the source of the food-borne outbreak...
March 2018: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
Anyun Zhang, Yunxia Li, Zhongbin Guan, Hongmei Tuo, Dan Liu, Yanxian Yang, Changwen Xu, Changwei Lei, Hongning Wang
The aim of this study was to evaluate the influence of apramycin administration on the development of antibiotic resistance in Escherichia coli ( E. coli ) strains isolated from chicken feces and houseflies under field conditions. Chickens in the medicated group ( n = 25,000) were given successive prophylactic doses (0.5 mg/l) of apramycin in their drinking water from Days 1 to 5, while no antibiotics were added to the un-medicated groups drinking water ( n = 25,000). Over 40 days, a total of 1170 E. coli strains were isolated from fecal samples obtained from medicated and un-medicated chickens and houseflies from the same chicken farm...
2018: Frontiers in Microbiology
Zofia Bakuła, Anna Brzostek, Paulina Borówka, Anna Żaczek, Izabela Szulc-Kiełbik, Agata Podpora, Paweł Parniewski, Dominik Strapagiel, Jarosław Dziadek, Małgorzata Proboszcz, Jacek Bielecki, Jakko van Ingen, Tomasz Jagielski
Molecular epidemiological studies of Mycobacterium kansasii are hampered by the lack of highly-discriminatory genotyping modalities. The purpose of this study was to design a new, high-resolution fingerprinting method for M. kansasii. Complete genome sequence of the M. kansasii ATCC 12478 reference strain was searched for satellite-like repetitive DNA elements comprising tandem repeats. A total of 24 variable-number tandem repeat (VNTR) loci were identified with potential discriminatory capacity. Of these, 17 were used to study polymorphism among 67 M...
March 13, 2018: Scientific Reports
Eric Y Kok, Jesus G Vallejo, Lauren M Sommer, Louie Rosas, Sheldon L Kaplan, Kristina G Hulten, J Chase McNeil
Background : Methicillin-resistant Staphylococcus aureus (MRSA), particularly those belonging to the USA300 pulsotype, have been well described to cause severe osteoarticular infections (OAI). Vancomycin MIC ≥ 1.5 μg/ml has been demonstrated to contribute to disease severity in adults with MRSA and even MSSA bacteremia. Little data exists describing the outcomes of MSSA OAI in terms of molecular characteristics and vancomycin MIC. Methods : All patients/isolates were chosen from a surveillance study at Texas Children's Hospital (TCH)...
March 12, 2018: Antimicrobial Agents and Chemotherapy
Carole Ayoub Moubareck, Shaimaa F Mouftah, Tibor Pál, Akela Ghazawi, Dalal H Halat, Anju Nabi, Mouza A AlSharhan, Zulfa O AlDeesi, Christabel C Peters, Handan Celiloglu, Manjunath Sannegowda, Dolla K Sarkis, Ágnes Sonnevend
Few studies have addressed the molecular epidemiology of carbapenem resistant Enterobacteriaceae (CRE) isolates in the Arabian Peninsula, and such investigations have been missing from Dubai, a major economical, tourism and medical centre of the region. The antibiotic susceptibility, the carbapenemase type produced, and the clonality of 89 CRE strains isolated in five major Dubai hospitals in June 2015 - June 2016 were determined. Thirty three percent of the collection of 70 K. pneumoniae, 13 E. coli and 6 other Enterobacteriaceae were extremely drug resistant, 27% were resistant to colistin, and 4...
March 9, 2018: International Journal of Antimicrobial Agents
Paola Caruso, Elena G Biosca, Edson Bertolini, Ester Marco-Noales, María Teresa Gorris, Concetta Licciardello, María M López
The characterization and intraspecific diversity of a collection of 45 Ralstonia solanacearum strains isolated in Spain from different sources and geographical origins is reported. To test the influence of the site and the host on strain diversity, phenotypic and genotypic analysis were performed by a polyphasic approach. Biochemical and metabolic profiles were compared. Serological relationship was evaluated by Indirect-ELISA using polyclonal and monoclonal antibodies. For genotypic analysis, hrpB and egl DNA sequence analysis, repetitive sequences (rep-PCR), amplified fragment length polymorphism (AFLP) profiles and macrorestriction with XbaI followed by pulsed field gel electrophoresis (PFGE) were performed...
December 2017: International Microbiology: the Official Journal of the Spanish Society for Microbiology
Inés Bado, Romina Papa Ezdra, Nicolás Cordeiro, Matilde Outeda, Leticia Caiata, Virginia García-Fulgueiras, Verónica Seija, Rafael Vignoli
OBJECTIVES: The objective of the present study was to characterise the mechanisms underlying quinolone and oxyimino-cephalosporin resistance in a Citrobacter freundii clinical isolate obtained from the ICU in Uruguay's University Hospital. METHODS: Citrobacter freundii strain CF638 was isolated from a urine culture. Identification and susceptibility testing were performed using the VITEK® 2 system, and MIC determination and disc diffusion assay, respectively. Resistance genes and mobile genetic elements were identified, by PCR and sequencing...
March 8, 2018: Journal of Global Antimicrobial Resistance
Caroline Vincent, Valentine Usongo, Chrystal Berry, Denise M Tremblay, Sylvain Moineau, Khadidja Yousfi, Florence Doualla-Bell, Eric Fournier, Céline Nadon, Lawrence Goodridge, Sadjia Bekal
Salmonella enterica serovar Heidelberg (S. Heidelberg) is one of the top serovars causing human salmonellosis. This serovar ranks second and third among serovars that cause human infections in Québec and Canada, respectively, and has been associated with severe infections. Traditional typing methods such as PFGE do not display adequate discrimination required to resolve outbreak investigations due to the low level of genetic diversity of isolates belonging to this serovar. This study evaluates the ability of four whole genome sequence (WGS)-based typing methods to differentiate among 145 S...
August 2018: Food Microbiology
Giancarlo Ripabelli, Manuela Tamburro, Giuliana Guerrizio, Incoronata Fanelli, Romeo Flocco, Massimiliano Scutellà, Michela L Sammarco
Antimicrobial-resistant Klebsiella pneumoniae represent a global public health concern. K. pneumoniae strains isolated during 2010 and 2014-2016 within a single hospital of Molise Region, Central Italy, were analyzed testing antimicrobial susceptibility, clonality by pulsed-field gel electrophoresis (PFGE) and random amplified polymorphic DNA (RAPD)-PCR, and prevalence of carbapenem resistance genes by PCR. Forty isolates (23 wild-type in 2010 and 17 non-wild-type in 2014-2016) were collected from hospitalized patients (65...
March 9, 2018: Current Microbiology
Yongming Zhou, Jingyun Zhang, Shukun Wang, Wen Xu, Weili Liang, Meiying Yan, Duochun Wang, Baowei Diao, Bo Pang, Xin Lu, Fenxia Fan, Jie Li, Jing Lou, Li Zhang, Ruibai Wang, Xiaoying Cui, Meng Zhao, Rui Wu, Hongyan Cai, Xiaoli Du, Zhigang Cui, Wenpeng Gu, Rusong Yang, Biao Kan
OBJECTIVES: To conduct a one-year pathogen surveillance of acute diarrheal disease based on outpatient clinics in township hospitals in rural Hongta District of Yunnan Province, China. METHODS: Fecal specimens of acute diarrhea cases and relevant epidemiological information were collected. Salmonella, Shigella, Vibrio, Aeromonas, Plesiomonas shigelloides and diarrheogenic Escherichia coli (DEC) were examined. RESULTS: Among the 797 stool specimens sampled, 198 samples (24...
March 5, 2018: International Journal of Infectious Diseases: IJID
Hamid Solgi, Farzad Badmasti, Christian G Giske, Shadi Aghamohammad, Fereshteh Shahcheraghi
Objectives: Despite the fact that the blaOXA-48 and blaNDM-1 genes have successfully disseminated among Klebsiella pneumoniae isolates worldwide, outbreaks remain unidentified in Iran. Here we examined the molecular epidemiology of 96 carbapenem-resistant K. pneumoniae recovered from an Iranian hospital. Methods: A total of 96 non-replicate carbapenem-resistant K. pneumoniae were recovered from clinical specimens in a university hospital. Detection of ESBLs and carbapenemases produced by studied strains was performed using PCR and DNA sequencing...
March 6, 2018: Journal of Antimicrobial Chemotherapy
Qiu E Yang, Siham Rajab Agouri, Jonathan Mark Tyrrell, Timothy Rutland Walsh
The occurrence of heavy metal resistance genes in multi-resistant Enterobacteriaceae possessing bla NDM-1 or bla CTX-M-15 genes were examined by PCR and S1-PFGE. When compared with clinical susceptible isolates (10.0-30.0%), the pcoA, merA, silC and arsA genes occurred with higher frequencies in bla NDM-1 (48.8-71.8%) and bla CTX-M-15 (19.4-52.8%) positive isolates, and they are mostly located on plasmids. Given the high association of metal resistance genes with multidrug resistant Enterobacteriaceae, the use of heavy metals in hospitals and the environment needs increased vigilance...
March 5, 2018: Antimicrobial Agents and Chemotherapy
Maria Giufrè, Giulia Errico, Marisa Accogli, Monica Monaco, Laura Villa, Maria Antonietta Distasi, Tito Del Gaudio, Annalisa Pantosti, Alessandra Carattoli, Marina Cerquetti
The emergence of carbapenemase-producing Enterobacteriaceae (CPE) is a critical concern worldwide. In Italy, CPE isolates are very frequent with the KPC types strongly predominant whereas the New Delhi metallo-β-lactamase (NDM) enzymes are extremely rare. In this study, we report the first detection of NDM-5-producing Escherichia coli sequence type (ST) 167 isolates from two patients with urinary tract infection (Ec001and Ec002 from urines) including one with colonization (Ec003 from faeces) admitted to the same hospital in 2017 two months apart...
February 28, 2018: International Journal of Antimicrobial Agents
Satoshi Nakano, Miki Nagao, Tomomi Yamasaki, Hiroyuki Morimura, Natsuki Hama, Yoshio Iijima, Hiroto Shinomiya, Michio Tanaka, Masaki Yamamoto, Yasufumi Matsumura, Shiro Miyake, Satoshi Ichiyama
The early detection of Shiga toxin-producing Escherichia coli (STEC) is important for early diagnosis and preventing the spread of STEC. Although the confirmatory test for STEC should be based on the detection of Shiga toxin using molecular analysis, isolation permits additional characterization of STEC using a variety of methods, including O:H serotyping. The conventional slide agglutination O-antigen serogrouping used in many clinical laboratories is laborious and time-consuming. Surface plasmon resonance (SPR)-based immunosensors are commonly used to investigate a large variety of bio-interactions such as antibody/antigen, peptide/antibody, DNA/DNA, and antibody/bacteria interactions...
February 28, 2018: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
Falguni Debnath, Asish K Mukhopadhyay, Goutam Choudhury, Rudra Narayan Saha, Shanta Dutta
A foodborne acute gastroenteritis outbreak due to Shigella sonnei infection occurred in a household after having food in a housewarming party of Pakapol Village, South 24 Parganas District of West Bengal, an Indian state in November 2016. Here we report, the epidemiological and microbiological findings of this outbreak. Thirty-four people attended the party on 23rd November 2016 and had lunch together. Median incubation period for development of acute gastroenteritis (AGE) cases from time of food consumption was 18...
February 28, 2018: Japanese Journal of Infectious Diseases
Alex-Mikael Barkoff, Jussi Mertsola, Denis Pierard, Tine Dalby, Silje Vermedal Hoegh, Sophie Guillot, Paola Stefanelli, Marjolein van Gent, Guy Berbers, Didrik F Vestrheim, Margrethe Greve-Isdahl, Lena Wehlin, Margaretha Ljungman, Norman K Fry, Kevin Markey, Kari Auranen, Qiushui He
One reason for increased pertussis incidence is the adaptation of Bordetella pertussis to vaccine-induced immunity by modulating its genomic structure. This study, EUpert IV, includes 265 isolates collected from nine European countries during 2012 to 2015 (n=265) and compares the results to previous EUpert I-III studies (1998-2009). The analyses included genotyping, serotyping and pulsed-field gel electrophoresis (PFGE) and multi-locus variable-number tandem repeat analysis (MLVA). Genotyping results showed only small variation among the common virulence genes of B...
February 28, 2018: Journal of Clinical Microbiology
Purnima Gogoi, Probodh Borah, Iftikar Hussain, Leena Das, Girin Hazarika, Shantanu Tamuly, Luit Moni Barkalita
Twelve Salmonella isolates belonging to different serovars, viz. S. Enteritidis (4), S. Weltevreden (4), S Newport (1), S. Litchifield (1) and untypable strains (2) were isolated out of 332 diarrhoeic faecal samples collected from animals, birds and human. Of the two molecular typing methods, viz REP-PCR (Repetitive Element sequence-based polymerase chain reaction) and PFGE (Pulsed-field gel electrophoresis) applied, PFGE could clearly differentiate the strains belonging to different serovars as well as between the strains of the same serovar with respect to their source of isolation, while REP-PCR could not differentiate between strains of the same serovar...
February 28, 2018: Journal of Clinical Microbiology
Takahiro Yamaguchi, Ryuji Kawahara, Chihiro Katsukawa, Masashi Kanki, Tetsuya Harada, Shinya Yonogi, Satomi Iwasaki, Hirokazu Uehara, Saori Okajima, Hiroshi Nishimura, Kazushi Motomura, Masaya Miyazono, Yuko Kumeda, Kentaro Kawatsu
In September 2016, 140 patients with primary symptoms of sore throat and fever were identified in a school dormitory in Osaka, Japan. Epidemiological and laboratory investigations determined that this symptomatic condition was a food-borne outbreak of group G Streptococcus (GGS), with GGS being isolated from samples from patients, cooks and foods. The strain of GGS was identified as Streptococcus dysgalactiae subsp. equisimilis (SDSE) of two emm types ( stG 652.0 and st C36.0). The causative food, a broccoli salad, was contaminated with the two types of SDSE, totaling 1...
February 28, 2018: Journal of Clinical Microbiology
A Peirotén, J L Arqués, M Medina, E Rodríguez-Mínguez
Importance of bifidobacteria as part of the infant intestinal microbiota has been highlighted. Their acquisition is influenced by the mode of birth and the feed regime afterwards, with a special role of the maternal microbiota. The presence of the same shared bifidobacterial strains between breast milk and infant faeces in 14 mother-infant pairs was assessed by means of pulsed-field gel electrophoresis (PFGE) genotyping. Four shared strains of Bifidobacterium breve (2), Bifidobacterium longum subsp. infantis and B...
February 27, 2018: Beneficial Microbes
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