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Triple a

Simone Cristoni, Guglielmo Dusi, Paolo Brambilla, Adriana Albini, Matteo Conti, Maura Brambilla, Antonino Bruno, Francesca Digaudio, Luca Ferlin, Valeria Tazzari, Silvia Mengozzi, Simone Barera, Carlos Salier, Tommaso Trenti, Marco Cantu, Luigi Rossi Bernardi, Douglas M Noonan
Electrospray Ionization (EI) and Collision Induced Dissociation tandem mass spectrometry (CID-MS/MS) are usually employed to obtain compound identification through a mass spectra match. Different algorithms have been developed for this purpose (for example the NIST match algorithm). These approaches compare the tandem mass spectra (MS/MS) of the unknown analyte with the MS/MS spectra of known compounds inserted in a database. The compounds are usually identified on the basis of spectral match value associated with a probability of recognition...
October 24, 2016: Journal of Mass Spectrometry: JMS
Hussain Mohamad Awwad, Juergen Geisel, Rima Obeid
BACKGROUND: Trimethylamine-N-oxide (TMAO) is produced in the liver from trimethylamine (TMA) and is an important cellular osmolyte and potential atherogenic factor. Taurine is involved in cholesterol metabolism and also serves as a cellular osmolyte. Given their significant biological functions, the development of reliable measurement techniques is crucial to further study their role in health and disease METHODS: A new ultrahigh performance liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous determination of TMA, TMAO, and taurine in plasma and urine...
October 15, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Jun Itakura, Masayuki Kurosaki, Chitomi Hasebe, Yukio Osaki, Kouji Joko, Hitoshi Yagisawa, Shinya Sakita, Hiroaki Okushin, Takashi Satou, Hiroyuki Hisai, Takehiko Abe, Keiji Tsuji, Takashi Tamada, Haruhiko Kobashi, Akeri Mitsuda, Yasushi Ide, Chikara Ogawa, Syotaro Tsuruta, Kouichi Takaguchi, Miyako Murakawa, Yasuhiro Asahina, Nobuyuki Enomoto, Namiki Izumi
BACKGROUNDS & AIMS: We aimed to clarify the characteristics of resistance-associated substitutions (RASs) after treatment failure with NS5A inhibitor, daclatasvir (DCV) in combination with NS3/4A inhibitor, asunaprevir (ASV), in patients with chronic hepatitis C virus genotype 1b infection. METHODS: This is a nationwide multicenter study conducted by the Japanese Red Cross Liver Study Group. The sera were obtained from 68 patients with virological failure after 24 weeks of DCV/ASV treatment...
2016: PloS One
Katrin Ehlers, Amey S Bhide, Dawit G Tekleyohans, Benjamin Wittkop, Rod J Snowdon, Annette Becker
Seed formation is a pivotal process in plant reproduction and dispersal. It begins with megagametophyte development in the ovule, followed by fertilization and subsequently coordinated development of embryo, endosperm, and maternal seed coat. Two closely related MADS-box genes, SHATTERPROOF 1 and 2 (SHP1 and SHP2) are involved in specifying ovule integument identity in Arabidopsis thaliana. The MADS box gene ARABIDOPSIS BSISTER (ABS or TT16) is required, together with SEEDSTICK (STK) for the formation of endothelium, part of the seed coat and innermost tissue layer formed by the maternal plant...
2016: PloS One
Dai Horiuchi, Roman Camarda, Alicia Y Zhou, Christina Yau, Olga Momcilovic, Sanjeev Balakrishnan, Alexandra N Corella, Henok Eyob, Kai Kessenbrock, Devon A Lawson, Lindsey A Marsh, Brittany N Anderton, Julia Rohrberg, Ratika Kunder, Alexey V Bazarov, Paul Yaswen, Michael T McManus, Hope S Rugo, Zena Werb, Andrei Goga
Triple-negative breast cancer (TNBC), in which cells lack expression of the estrogen receptor (ER), the progesterone receptor (PR) and the ERBB2 (also known as HER2) receptor, is the breast cancer subtype with the poorest outcome. No targeted therapy is available against this subtype of cancer owing to a lack of validated molecular targets. We previously reported that signaling involving MYC-an essential, pleiotropic transcription factor that regulates the expression of hundreds of genes-is disproportionally higher in triple-negative (TN) tumors than in receptor-positive (RP) tumors...
October 24, 2016: Nature Medicine
Fara Brasó-Maristany, Simone Filosto, Steven Catchpole, Rebecca Marlow, Jelmar Quist, Erika Francesch-Domenech, Darren A Plumb, Leila Zakka, Patrycja Gazinska, Gianmaria Liccardi, Pascal Meier, Albert Gris-Oliver, Maggie Chon U Cheang, Anna Perdrix-Rosell, Manar Shafat, Elodie Noël, Nirmesh Patel, Kristen McEachern, Maurizio Scaltriti, Pau Castel, Farzana Noor, Richard Buus, Sumi Mathew, Johnathan Watkins, Violeta Serra, Pierfrancesco Marra, Anita Grigoriadis, Andrew N Tutt
Triple-negative breast cancers (TNBCs) have poor prognosis and lack targeted therapies. Here we identified increased copy number and expression of the PIM1 proto-oncogene in genomic data sets of patients with TNBC. TNBC cells, but not nonmalignant mammary epithelial cells, were dependent on PIM1 for proliferation and protection from apoptosis. PIM1 knockdown reduced expression of the anti-apoptotic factor BCL2, and dynamic BH3 profiling of apoptotic priming revealed that PIM1 prevents mitochondrial-mediated apoptosis in TNBC cell lines...
October 24, 2016: Nature Medicine
Lucie Pinasseau, Arnaud Verbaere, Maryline Roques, Emmanuelle Meudec, Anna Vallverdú-Queralt, Nancy Terrier, Jean-Claude Boulet, Véronique Cheynier, Nicolas Sommerer
A rapid, sensitive, and selective analysis method using ultra high performance liquid chromatography coupled with triple-quadrupole mass spectrometry (UHPLC-QqQ-MS) has been developed for the characterization and quantification of grape skin flavan-3-ols after acid-catalysed depolymerization in the presence of phloroglucinol (phloroglucinolysis). The compound detection being based on specific MS transitions in Multiple Reaction Monitoring (MRM) mode, this fast gradient robust method allows analysis of constitutive units of grape skin proanthocyanidins, including some present in trace amounts, in a single injection, with a throughput of 6 samples per hour...
October 21, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
M Fanjul, L Pérez, J Cerdá, M Zornoza, R Rojo, I Simal, M A García-Casillas, C Corona, D Peláez, E Molina, A Parente, S Rivas, J M Angulo, E De Tomás
BACKGROUND: Multiple approaches to the treatment of simple and complicated (gangrenous or perforated) appendicitis in children have been promoted. Our goal is to develop a new protocol for these patients that allows shorter hospital stays without increasing complications rates. METHODS: Prospective collected data of patients undergoing appendicitis treated according to the new protocol for a period of 7 months were reviewed. This protocol consists on antibiotic prophylaxis in all cases continued with triple antibiotic regimen in complicated appendicitis...
October 10, 2016: Cirugía Pediátrica: Organo Oficial de la Sociedad Española de Cirugía Pediátrica
Yawen Wang, Le Yu, Xiong Wen David Lou
Unique triple-shelled Mo-polydopamine (Mo-PDA) hollow spheres are synthesized through a facile solvothermal process. A sequential self-templating mechanism for the multi-shell formation is proposed, and the number of shells can be adjusted by tuning the size of the Mo-glycerate templates. These triple-shelled Mo-PDA hollow spheres can be converted to triple-shelled MoO2 /carbon composite hollow spheres by thermal treatment. Owing to the unique multi-shells and hollow interior, the as-prepared MoO2 /carbon composite hollow spheres exhibit appealing performance as an anode material for lithium-ion batteries, delivering a high capacity of ca...
October 24, 2016: Angewandte Chemie
Ezgi Yalçıntaş, Andreas C Scheinost, Xavier Gaona, Marcus Altmaier
The mechanisms for the reduction and uptake of Tc by magnetite (Fe3O4) and mackinawite (FeS) are investigated using X-ray absorption spectroscopy (XANES and EXAFS), in combination with thermodynamic calculations of the Tc/Fe systems and accurate characterization of the solution properties (pHm, pe, [Tc]). Batch sorption experiments were performed under strictly anoxic conditions using freshly prepared magnetite and mackinawite in 0.1 M NaCl solutions with varying initial Tc(vii) concentrations (2 × 10(-5) and 2 × 10(-4) M) and Tc loadings (400-900 ppm)...
October 24, 2016: Dalton Transactions: An International Journal of Inorganic Chemistry
A Blomme, B Costanza, P de Tullio, M Thiry, G Van Simaeys, S Boutry, G Doumont, E Di Valentin, T Hirano, T Yokobori, S Gofflot, O Peulen, A Bellahcène, F Sherer, C Le Goff, E Cavalier, A Mouithys-Mickalad, F Jouret, P G Cusumano, E Lifrange, R N Muller, S Goldman, P Delvenne, E De Pauw, M Nishiyama, V Castronovo, A Turtoi
Myoferlin is a multiple C2-domain-containing protein that regulates membrane repair, tyrosine kinase receptor function and endocytosis in myoblasts and endothelial cells. Recently it has been reported as overexpressed in several cancers and shown to contribute to proliferation, migration and invasion of cancer cells. We have previously demonstrated that myoferlin regulates epidermal growth factor receptor activity in breast cancer. In the current study, we report a consistent overexpression of myoferlin in triple-negative breast cancer cells (TNBC) over cells originating from other breast cancer subtypes...
October 24, 2016: Oncogene
A Chiche, M Moumen, M Romagnoli, V Petit, H Lasla, P Jézéquel, P de la Grange, J Jonkers, M-A Deugnier, M A Glukhova, M M Faraldo
Triple-negative breast cancer is a heterogeneous disease characterized by the expression of basal cell markers, no estrogen or progesterone receptor expression and a lack of HER2 overexpression. Triple-negative tumors often display activated Wnt/β-catenin signaling and most have impaired p53 function. We studied the interplay between p53 loss and Wnt/β-catenin signaling in stem cell function and tumorigenesis, by deleting p53 from the mammary epithelium of K5ΔNβcat mice displaying a constitutive activation of Wnt/β-catenin signaling in basal cells...
October 24, 2016: Oncogene
Rüdiger Bertermann, Holger Braunschweig, Mehmet Ali Celik, Theresa Dellermann, Hauke Kelch
The reaction of the vicinal biscarbenoid Pip-C[triple bond, length as m-dash]C-Pip with dimethyltin dichloride yields a unique tetraamino-substituted cyclobutadienyl system featuring a dative C-Sn interaction. DFT investigation of the reaction mechanism revealed that coordination of the stannyl fragment to the nucleophilic carbon leads to a metal-stabilised zwitterion, allowing for [2+2] cycloaddition under thermal conditions. The compound features a homoaromatic π-system comprising the three sp(2)-hybridised carbon atoms of the four-membered ring as a consequence of charge separation...
October 24, 2016: Chemical Communications: Chem Comm
Yan Duan, Juan M Clemente-Juan, José L G Fierro, Carlos Giménez-Saiz, Eugenio Coronado
Partial reductive hydrolysis of a penta-Co(II/III) cluster [Co(H2O)2(Co(III)W9O34)(PW9O34)](12-) (1) leads to the formation of [Co2{Co3(H2O)(Co(OH)2W7O26)(PW9O34)}2](22-) (2). This polyoxometalate is made up of two capping [PW9O34](9-) units and two bridging [W7O26](10-) units that assemble to encapsulate a novel deca-Co(II) cluster core comprising octahedral and tetrahedral Co(II) ions.
October 24, 2016: Chemical Communications: Chem Comm
Yan Guo, Jie Wu, Shilin Zhao, Fei Ye, Yinghao Su, Travis Clark, Quanhu Sheng, Brian Lehmann, Xiao-Ou Shu, Qiuyin Cai
Background. Proper rRNA depletion is crucial for the successful utilization of FFPE specimens when studying gene expression. We performed a study to evaluate two major rRNA depletion methods: Ribo-Zero and RNase H. RNAs extracted from 4 samples were treated with the two rRNA depletion methods in duplicate and sequenced (N = 16). We evaluated their reducibility, ability to detect RNA, and ability to molecularly subtype these triple negative breast cancer specimens. Results. Both rRNA depletion methods produced consistent data between the technical replicates...
2016: International Journal of Genomics
Shekhar K Gadkaree, Daniel Q Sun, Carol Li, Frank R Lin, Luigi Ferrucci, Eleanor M Simonsick, Yuri Agrawal
Objectives. To investigate whether sensory function declines independently or in parallel with age within a single individual. Methods. Cross-sectional analysis of Baltimore Longitudinal Study of Aging (BLSA) participants who underwent vision (visual acuity threshold), proprioception (ankle joint proprioceptive threshold), vestibular function (cervical vestibular-evoked myogenic potential), hearing (pure-tone average audiometric threshold), and Health ABC physical performance battery testing. Results. A total of 276 participants (mean age 70 years, range 26-93) underwent all four sensory tests...
2016: Journal of Aging Research
Linyong Song, Qiang Ye, Xueping Ge, Anil Misra, Candan Tamerler, Paulette Spencer
A self-strengthening methacrylate-based dental adhesive system was developed by introducing an epoxy cyclohexyl trimethoxysilane (TS) which contains both epoxy and methoxysilyl functional groups. The experimental formulation, HEMA/BisGMA/TS (22.5/27.5/50, wt%), was polymerized by visible-light. Real-time Fourier transform infrared spectroscopy (FTIR) was used to investigate in situ the free radical polymerization of methacrylate, ring-opening cationic polymerization of epoxy, and photoacid-induced sol-gel reactions...
2016: RSC Advances
Grace Thompson, Mina John, Abha Chopra
No abstract text is available yet for this article.
February 2016: Pathology
Rhiannon K Beckers, Christina Selinger, Ricardo Vilain, Jason Madore, James S Wilmott, Kate Harvey, Anne Holliday, Caroline L Cooper, Elizabeth Robbins, David Gillet, Catherine W Kennedy, Laurence Gluch, Hugh Carmalt, Cindy Mak, Sanjay Warrier, Harriet E Gee, Charles Chan, Anna McLean, Emily Walker, Catriona M McNeil, Jane M Beith, Alexander Swarbrick, Richard A Scolyer, Sandra A O'Toole
No abstract text is available yet for this article.
February 2016: Pathology
Tatsuya Kaji, Osamu Yamasaki, Minoru Takata, Masaki Otsuka, Toshihisa Hamada, Shin Morizane, Kenji Asagoe, Hiroyuki Yanai, Yoji Hirai, Hiroshi Umemura, Keiji Iwatsuki
BACKGROUND: Searching for driver mutations in melanoma is critical to understanding melanoma genesis, progression and response to therapy. OBJECTIVES: We aimed to investigate the frequency and pattern of driver mutations in Japanese primary and metastatic melanomas including cases of unknown primary origin, in relation to their clinicopathologic manifestations. METHODS: Seventy-seven samples from 60 patients with melanoma were screened for 70 driver mutations of 20 oncogenes by Sequenom MelaCarta MassARRAY, and the results for primary and metastatic melanomas were compared...
October 13, 2016: Journal of Dermatological Science
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