keyword
MENU ▼
Read by QxMD icon Read
search

Yeast two hybrid

keyword
https://www.readbyqxmd.com/read/28736570/genome-wide-identification-and-functional-analysis-of-the-calcineurin-b-like-protein-and-calcineurin-b-like-protein-interacting-protein-kinase-gene-families-in-turnip-brassica-rapa-var-rapa
#1
Xin Yin, Qiuli Wang, Qian Chen, Nan Xiang, Yunqiang Yang, Yongping Yang
The calcineurin B-like protein (CBL)-CBL-interacting protein kinase (CIPK) complex has been identified as a primary component in calcium sensors that perceives various stress signals. Turnip (Brassica rapa var. rapa) has been widely cultivated in the Qinghai-Tibet Plateau for a century as a food crop of worldwide economic significance. These CBL-CIPK complexes have been demonstrated to play crucial roles in plant response to various environmental stresses. However, no report is available on the genome-wide characterization of these two gene families in turnip...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28735869/dhu1-negatively-regulates-uv-b-signaling-via-its-direct-interaction-with-cop1-and-rup1
#2
Sang-Hoon Kim, Hani Kim, Sunglan Chung, Jae-Hoon Lee
Although DWD HYPERSENSITIVE TO UV-B 1 (DHU1) is reported to be a negative regulator in UV-B mediated cellular responses, its detailed role in UV-B signaling is still elusive. To further understand the action mechanism of DHU1 in UV-B response, physical and genetic interactions of DHU1 with various UV-B signaling components were investigated. Yeast two hybrid assay results suggested that DHU1 directly interacts with COP1 and RUP1, implying a functional connection with both COP1 and RUP1. In spite of the physical association between DHU1 and COP1, loss of DHU1 did not affect protein stability of COP1...
July 20, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28728781/a-yeast-two-hybrid-assay-reveals-cmya1-interacting-proteins
#3
Xiangbo Xin, Ting Wang, Xinfeng Liu, Guoning Sui, Congfei Jin, Yingwei Yue, Shuping Yang, Hong Guo
CMYA1 is a protein that plays an important role in muscle development and contains a highly conserved Xin repeats region. However, the function of the Xin-repeats in CMYA1 is unknown, and there is little information regarding proteins that interact with the CMYA1/Xin-repeats in the bovine system. In this study, we generated a high-quality bovine muscle cDNA library and performed a yeast two-hybrid screen twice using both CMYA1 and the Xin-repeats as bait. There were 27 candidate-interacting proteins identified in this screen...
July 17, 2017: Comptes Rendus Biologies
https://www.readbyqxmd.com/read/28726545/the-t-6-a-modification-acts-as-a-positive-determinant-for-the-anticodon-nuclease-prrc-and-is-distinctively-nonessential-in-streptococcus-mutans
#4
Jo Marie Bacusmo, Silvia S Orsini, Jennifer Hu, Michael DeMott, Patrick C Thiaville, Ameer Elfarash, Mellie June Paulines, Diego Rojas-Benítez, Birthe Meineke, Chris Deutsch, Dirk Iwata-Reuyl, Patrick A Limbach, Peter C Dedon, Kelly C Rice, Stewart Shuman, Valérie de Crécy-Lagard
Endoribonuclease toxins (ribotoxins) are produced by bacteria and fungi to respond to stress, eliminate non-self competitor species, or interdict virus infection. PrrC is a bacterial ribotoxin that targets and cleaves tRNA(Lys)UUU in the anticodon loop. In vitro studies suggested that the post-transcriptional modification threonylcarbamoyl adenosine (t(6)A) is required for PrrC activity but this prediction had never been validated in vivo. Here, by using t(6)A-deficient yeast derivatives, it is shown that t(6)A is a positive determinant for PrrC proteins from various bacterial species...
July 20, 2017: RNA Biology
https://www.readbyqxmd.com/read/28726294/screening-and-structural-and-functional-investigation-of-a-novel-ferritin-from-phascolosoma-esculenta
#5
Hongwei Ding, Dijun Zhang, Shuangshuang Chu, Jun Zhou, Xiurong Su
Ferritins are primary iron storage proteins and play a crucial role in iron storage and detoxification. Yeast two-hybrid method was employed to screen the cDNA library of Phascolosoma esculenta. Sequence of positive colony FER147 was analyzed. The higher similarity and conserved motifs for ferritin indicated that it belonged to a new member of ferritin family. The interaction between Ferritin and Fer147 was further confirmed through co-immunoprecipitation. The pET-28a-FER147 prokaryotic expression vector was constructed...
July 20, 2017: Protein Science: a Publication of the Protein Society
https://www.readbyqxmd.com/read/28724759/cholesterol-25-hydroxylase-inhibits-porcine-reproductive-and-respiratory-syndrome-virus-replication-through-enzyme-activity-dependent-and-independent-mechanisms
#6
Wenting Ke, Liurong Fang, Huiyuan Jing, Ran Tao, Ting Wang, Yang Li, Siwen Long, Dang Wang, Shaobo Xiao
Cholesterol 25-hydroxylase (CH25H) has recently been identified as a host restriction factor that exerts antiviral effects by catalyzing the production of 25-hydroxycholesterol (25HC). CH25H can be rapidly induced upon infection with some viruses. Porcine reproductive and respiratory syndrome virus (PRRSV), an arterivirus, has ranked among the most important swine pathogens since it was discovered in the late 1980s. In this study, we found that PRRSV infection significantly downregulated the expression of CH25H in cells by a so far unknown mechanism, suggesting that CH25H might exert antiviral activity against PRRSV...
July 19, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28722263/ftsad2-and-ftjaz1-regulate-activity-of-the-ftmyb11-transcription-repressor-of-the-phenylpropanoid-pathway-in-fagopyrum-tataricum
#7
Meiliang Zhou, Zhanmin Sun, Mengqi Ding, Maria D Logacheva, Ivan Kreft, Dan Wang, Mingli Yan, Jirong Shao, Yixiong Tang, Yanmin Wu, Xuemei Zhu
Little is known about the molecular mechanism of the R2R3-MYB transcriptional repressors involved in plant phenylpropanoid metabolism. Here, we describe one R2R3-type MYB repressor, FtMYB11 from Fagopyrum tataricum. It contains the SID-like motif GGDFNFDL and it is regulated by both the importin protein 'Sensitive to ABA and Drought 2' (SAD2) and the jasmonates signalling cascade repressor JAZ protein. Yeast two hybrid and bimolecular fluorescence complementation assays demonstrated that FtMYB11 interacts with SAD2 and FtJAZ1...
July 19, 2017: New Phytologist
https://www.readbyqxmd.com/read/28721853/us10-a-novel-npro-interacting-protein-inhibits-classical-swine-fever-virus-replication
#8
Huifang Lv, Wang Dong, Gui Qian, Jie Wang, Xiaomeng Li, Zhi Cao, Qizhuang Lv, Chengbao Wang, Kangkang Guo, Yanming Zhang
Classical swine fever (CSF) is a severe, febrile and highly contagious disease caused by classical swine fever virus (CSFV) that has resulted in huge economic losses in the pig industry worldwide. CSFV Npro has been actively studied but remains incompletely understood. Few studies have investigated the cellular proteins that interact with Npro and their participation in viral replication. Here, the yeast two-hybrid (Y2H) system was employed to screen Npro-interacting proteins from a porcine alveolar macrophage (PAM) cDNA library, and a blast search of the NCBI database revealed that 15 cellular proteins interact with Npro...
July 18, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28720704/cytosolic-interaction-of-type-iii-human-cd38-with-cib1-modulates-cellular-cyclic-adp-ribose-levels
#9
Jun Liu, Yong Juan Zhao, Wan Hua Li, Yun Nan Hou, Ting Li, Zhi Ying Zhao, Cheng Fang, Song Lu Li, Hon Cheung Lee
CD38 catalyzes the synthesis of the Ca(2+) messenger, cyclic ADP-ribose (cADPR). It is generally considered to be a type II protein with the catalytic domain facing outside. How it can catalyze the synthesis of intracellular cADPR that targets the endoplasmic Ca(2+) stores has not been resolved. We have proposed that CD38 can also exist in an opposite type III orientation with its catalytic domain facing the cytosol. Here, we developed a method using specific nanobodies to immunotarget two different epitopes simultaneously on the catalytic domain of the type III CD38 and firmly established that it is naturally occurring in human multiple myeloma cells...
July 18, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28717008/the-cardiac-z-disc-protein-cefip-regulates-cardiomyocyte-hypertrophy-by-modulating-calcineurin-signaling
#10
Franziska Dierck, Christian Kuhn, Claudia Rohr, Susanne Hille, Julia Braune, Samuel Sossalla, Sibylle Molt, Peter F M van der Ven, Dieter O Fürst, Norbert Frey
The z-disc is a structural component at the lateral borders of the sarcomere and is important for mechanical stability and contractility of both cardiac and skeletal muscles. Of note, the sarcomeric z-disc also represents a nodal point in cardiomyocyte function and signaling. Mutations of numerous z-disc proteins are associated with cardiomyopathies and muscle diseases. To identify additional z-disc proteins that might contribute to cardiac disease, we employed an in silico screen for cardiac-enriched cDNAs...
July 17, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28713394/a-novel-nac-transcription-factor-pbenac1-of-pyrus-betulifolia-confers-cold-and-drought-tolerance-via-interacting-with-pbedrebs-and-activating-the-expression-of-stress-responsive-genes
#11
Cong Jin, Kong-Qing Li, Xiao-Yong Xu, Hu-Ping Zhang, Hui-Xian Chen, Yu-Hong Chen, Jing Hao, Yang Wang, Xiao-San Huang, Shao-Ling Zhang
NAC (NAM, ATAF, and CUC) transcription factors are important regulator in abiotic stress and plant development. However, knowledge concerning the functions of plant NAC TFs functioning in stress tolerance and the underlying molecular basis are still limited. In this study, we report functional characterization of the NAC TF, PbeNAC1, isolated from Pyrus betulifolia. PbeNAC1 were greatly induced by cold and drought, while salt stress had little effect on expression. PbeNAC1 was localized in the nuclei showed transactivation activity...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28710879/the-transcription-factor-pstste12-is-required-for-virulence-of-puccinia-striiformis-f-sp-tritici
#12
Xiaoguo Zhu, Wei Liu, Xiuling Chu, Qixiong Sun, Chenglong Tan, Qian Yang, Min Jiao, Jun Guo, Zhensheng Kang
Puccinia striiformis f. sp. tritici (Pst) is an obligate biotrophic fungus that causes extensive damage in wheat. The pathogen is now known to be a heteroecious fungus with an intricate life cycle containing sexual and asexual stages. Orthologs of the STE12 transcription factor that regulate mating and filamentation in Saccharomyces cerevisiae as well as virulence in other fungi has been extensively described. Because reliable transformation and gene disruption methods are lacking for Pst, knowledge about the function of its STE12 ortholog is limited...
July 15, 2017: Molecular Plant Pathology
https://www.readbyqxmd.com/read/28710291/two-types-of-etiological-mutation-in-the-limb-specific-enhancer-of-shh
#13
Takanori Amano, Tomoko Sagai, Ryohei Seki, Toshihiko Shiroishi
An enhancer named MFCS1 regulates Sonic hedgehog (Shh) expression in the posterior mesenchyme of limb buds. Several mutations in MFCS1 induce ectopic Shh expression in the anterior limb bud, and these result in preaxial polydactyly (PPD). However, the molecular basis of ectopic Shh expression remains elusive, although some mutations are known to disrupt the negative regulation of Shh expression in the anterior limb bud. Here, we analyzed the molecular mechanism of ectopic Shh expression in PPD including in a mouse mutation-hemimelic extra toes (Hx)-and in other MFCS1 mutations in different species...
July 14, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/28702045/functional-characterization-of-tafusca3-a-b3-superfamily-transcription-factor-gene-in-the-wheat
#14
Fusheng Sun, Xiyan Liu, Qiuhui Wei, Jiannan Liu, Tianxiang Yang, Liyang Jia, Yuesheng Wang, Guangxiao Yang, Guangyuan He
The end-use quality of wheat, including its unique rheology and viscoelastic properties, is predominantly determined by the composition and concentration of gluten proteins. While, the mechanism regulating expression of the seed storage protein (SSP) genes and other related genes in wheat remains unclear. In this study, we report on the cloning and functional identification of TaFUSCA3, a B3-superfamily transcription factor (TF) gene in wheat. Sequence alignment indicated that wheat and barley FUSCA3 genes are highly conserved...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28701884/cis-prenyltransferase-interacts-with-a-nogo-b-receptor-homolog-for-dolichol-biosynthesis-in-panax-ginseng-meyer
#15
Ngoc Quy Nguyen, Sang-Choon Lee, Tae-Jin Yang, Ok Ran Lee
BACKGROUND: Prenyltransferases catalyze the sequential addition of isopentenyl diphosphate units to allylic prenyl diphosphate acceptors and are classified as either trans-prenyltransferases (TPTs) or cis-prenyltransferases (CPTs). The functions of CPTs have been well characterized in bacteria, yeast, and mammals compared to plants. The characterization of CPTs also has been less studied than TPTs. In the present study, molecular cloning and functional characterization of a CPT from a medicinal plant, Panax ginseng Mayer were addressed...
July 2017: Journal of Ginseng Research
https://www.readbyqxmd.com/read/28696213/ca-2-regulation-of-cav3-3-t-type-ca-2-channel-is-mediated-by-calmodulin
#16
Narae Lee, Sua Jeong, Kang-Chang Kim, Jin-Ah Kim, Jin-Yong Park, Ho-Won Kang, Edward Perez-Reyes, Jung-Ha Lee
Numerous investigations reported that increases of internal Ca(2+) (Ca(2+)i) pivotally regulate high voltage-activated (HVA) Ca(2+) channels via calmodulin (CaM). However, it is largely elusive that Ca(2+)i can regulate low voltage-activated T-type Ca(2+) channels. Using whole cell patch clamp, we compared the biophysical properties of Ca(2+) current through T-type Ca(2+) channel Cav3.1, Cav3.2, or Cav3.3 stably expressed in HEK293 cells between internal solutions containing 27 nM and l μM free Ca(2+) Both activation and inactivation kinetics of Cav3...
July 10, 2017: Molecular Pharmacology
https://www.readbyqxmd.com/read/28695958/identification-and-characterization-of-the-autophagy-related-genes-atg12-and-atg5-in-hydra
#17
Nishikant S Dixit, Bhupendra V Shravage, Surendra Ghaskadbi
Autophagy is an evolutionarily conserved process in eukaryotic cells that is involved in the degradation of cytoplasmic contents including organelles via the lysosome. Hydra is an early metazoan which exhibits simple tissue grade organization, a primitive nervous system, and is one of the classical non-bilaterian models extensively used in evo-devo research. Here, we describe the characterization of two core autophagy genes, Atg12 and Atg5, from hydra. In silico analyses including sequence similarity, domain analysis, and phylogenetic analysis demonstrate the conservation of these genes across eukaryotes...
2017: International Journal of Developmental Biology
https://www.readbyqxmd.com/read/28695742/the-human-regulatory-protein-ki-1-57-is-a-target-of-sumoylation-and-affects-pml-nuclear-body-formation
#18
Ângela Saito, Edmarcia E de Souza, Fernanda C Costa, Gabriela V Meirelles, Kaliandra de Almeida Gonçalves, Marcos T Santos, Gustavo C Bressan, Mark E McComb, Catherine E Costello, Stephen A Whelan, Jörg Kobarg
Ki-1/57 is a nuclear and cytoplasmic regulatory protein first identified in malignant cells from Hodgkin's lymphoma. It is involved in gene expression regulation on both transcriptional and mRNA metabolism levels. Ki-1/57 belongs to the family of intrinsically unstructured proteins, undergoes phosphorylation by PKC and methylation by PRMT1. Previous characterization of its protein interaction profile by yeast two-hybrid screening showed that Ki- 1/57 interacts with proteins of the SUMOylation machinery: the SUMO E2 conjugating enzyme UBC9 and the SUMO E3 ligase PIAS3, which suggested that Ki-1/57 could be involved with this process...
July 11, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28694796/evolutionary-diversification-of-alanine-transaminases-in-yeast-catabolic-specialization-and-biosynthetic-redundancy
#19
Ximena Escalera-Fanjul, Carlos Campero-Basaldua, Maritrini Colón, James González, Dariel Márquez, Alicia González
Gene duplication is one of the major evolutionary mechanisms providing raw material for the generation of genes with new or modified functions. The yeast Saccharomyces cerevisiae originated after an allopolyploidization event, which involved mating between two different ancestral yeast species. ScALT1 and ScALT2 codify proteins with 65% identity, which were proposed to be paralogous alanine transaminases. Further analysis of their physiological role showed that while ScALT1 encodes an alanine transaminase which constitutes the main pathway for alanine biosynthesis and the sole pathway for alanine catabolism, ScAlt2 does not display alanine transaminase activity and is not involved in alanine metabolism...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28694440/phylogenomic-analysis-of-mkks-and-mapks-from-16-legumes-and-detection-of-interacting-pairs-in-chickpea-divulge-mapk-signalling-modules
#20
Savithri Purayannur, Kamal Kumar, Vemula Chandra Kaladhar, Praveen Kumar Verma
The mitogen-activated protein kinase (MAPK)-mediated phosphorylation cascade is a vital component of plant cellular signalling. Despite this, MAPK signalling cascade is less characterized in crop legumes. To fill this void, we present here a comprehensive phylogeny of MAPK kinases (MKKs) and MAPKs identified from 16 legume species belonging to genistoid (Lupinus angustifolius), dalbergioid (Arachis spp.), phaseoloid (Glycine max, Cajanus cajan, Phaseolus vulgaris, and Vigna spp.), and galegoid (Cicer arietinum, Lotus japonicus, Medicago truncatula, Pisum sativum, Trifolium spp...
July 10, 2017: Scientific Reports
keyword
keyword
111912
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"