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https://www.readbyqxmd.com/read/28636910/prp40-homolog-a-is-a-novel-centrin-target
#1
Adalberto Díaz Casas, Walter J Chazin, Belinda Pastrana-Ríos
Pre-mRNA processing protein 40 (Prp40) is a nuclear protein that has a role in pre-mRNA splicing. Prp40 possesses two leucine-rich nuclear export signals, but little is known about the function of Prp40 in the export process. Another protein that has a role in protein export is centrin, a member of the EF-hand superfamily of Ca(2+)-binding proteins. Prp40 was found to be a centrin target by yeast-two-hybrid screening using both Homo sapiens centrin 2 (Hscen2) and Chlamydomonas reinhardtii centrin (Crcen). We identified a centrin-binding site within H...
June 20, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28635593/the-protein-slr1143-is-an-active-diguanylate-cyclase-in-synechocystis-sp-pcc-6803-and-interacts-with-the-photoreceptor-cph2
#2
Veronika Angerer, Philipp Schwenk, Thomas Wallner, Volkhard Kaever, Andreas Hiltbrunner, Annegret Wilde
Cyclic-di-GMP is an ubiquitous second messenger in bacteria. Several c-di-GMP receptor proteins have been identified to date, and downstream signalling pathways are often mediated through protein-protein interactions. The photoreceptor Cph2 from the cyanobacterium Synechocystis sp. PCC 6803 comprises three domains related to c-di-GMP metabolism: two GGDEF and one EAL domain. It has been shown that the C-terminal GGDEF domain acts as blue-light triggered c-di-GMP producer thereby inhibiting motility of the cells in blue light...
June 21, 2017: Microbiology
https://www.readbyqxmd.com/read/28633340/citrus-citnac62-cooperates-with-citwrky1-to-participate-in-citric-acid-degradation-via-up-regulation-of-citaco3
#3
Shao-Jia Li, Xue-Ren Yin, Wen-Li Wang, Xiao-Fen Liu, Bo Zhang, Kun-Song Chen
Citric acid is the predominant organic acid of citrus fruit. Degradation of citric acid occurs during fruit development, influencing fruit acidity. Associations of CitAco3 transcripts and citric acid degradation have been reported for citrus fruit. Here, transient overexpression of CitAco3 significantly reduced the citric acid content of citrus leaves and fruits. Using dual luciferase assays, it was shown that CitNAC62 and CitWRKY1 could transactivate the promoter of CitAco3. Subcellular localization results showed that CitWRKY1 was located in the nucleus and CitNAC62 was not...
June 15, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28633330/the-asparagine-rich-protein-nrp-interacts-with-the-verticillium-effector-pevd1-and-regulates-the-subcellular-localization-of-cryptochrome-2
#4
Ruimin Zhou, Tong Zhu, Lei Han, Mengjie Liu, Mengyuan Xu, Yanli Liu, Dandan Han, Dewen Qiu, Qingqiu Gong, Xinqi Liu
The soil-borne fungal pathogen Verticillium dahliae infects a wide range of dicotyledonous plants including cotton, tobacco, and Arabidopsis. Among the effector proteins secreted by V. dahliae, the 16 kDa PevD1 induces a hypersensitive response in tobacco. Here we report the high-resolution structure of PevD1 with folds resembling a C2 domain-like structure with a calcium ion bound to the C-terminal acidic pocket. A yeast two-hybrid screen, designed to probe for molecular functions of PevD1, identified Arabidopsis asparagine-rich protein (NRP) as the interacting partner of PevD1...
June 13, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28630074/the-enterohaemorrhagic-escherichia-coli-effector-espw-triggers-actin-remodeling-in-a-rac1-dependent-manner
#5
Pamela Sandu, Valerie F Crepin, Hauke Drechsler, Andrew D McAinsh, Gad Frankel, Cedric N Berger
Enterohaemorrhagic Escherichia coli (EHEC) is a diarrheagenic pathogen that colonizes the gut mucosa and induces attaching-and-effacing lesions. EHEC employs a type III secretion system (T3SS) to translocate 50 effector proteins that hijack and manipulate host cell signalling pathways, which allow bacterial colonization, subversion of immune responses and disease progression. The aim of this study was to characterize the T3SS effector EspW. We found espW in the sequenced O157:H7 and non-O157 EHEC strains as well as in Shigella boydii Furthermore, a truncated version of EspW, containing the first 206 residues is present in EPEC strains belonging to serotype O55:H7...
June 19, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28627360/the-plasmodium-falciparum-exported-protein-pf3d7_0402000-binds-to-erythrocyte-ankyrin-and-band-4-1
#6
Bikash Shakya, Wesley D Penn, Ernesto S Nakayasu, Douglas J LaCount
Plasmodium falciparum extensively modifies the infected red blood cell (RBC), resulting in changes in deformability, shape and surface properties. These alterations suggest that the RBC cytoskeleton is a major target for modification during infection. However, the molecular mechanisms leading to these changes are largely unknown. To begin to address this question, we screened for exported P. falciparum proteins that bound to the erythrocyte cytoskeleton proteins ankyrin 1 (ANK1) and band 4.1 (4.1R), which form critical interactions with other cytoskeletal proteins that contribute to the deformability and stability of RBCs...
June 13, 2017: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/28626465/altered-expression-of-osnla1-modulates-pi-accumulation-in-rice-oryza-sativa-l-plants
#7
Sihui Zhong, Kashif Mahmood, Yong-Mei Bi, Steven J Rothstein, Kosala Ranathunge
Current agricultural practices rely on heavy use of fertilizers for increased crop productivity. However, the problems associated with heavy fertilizer use, such as high cost and environmental pollution, require the development of crop species with increased nutrient use efficiency. In this study, by using transgenic approaches, we have revealed the critical role of OsNLA1 in phosphate (Pi) accumulation of rice plants. When grown under sufficient Pi and nitrate levels, OsNLA1 knockdown (Osnla1-1, Osnla1-2, and Osnla1-3) lines accumulated higher Pi content in their shoot tissues compared to wild-type, whereas, over-expression lines (OsNLA1-OE1, OsNLA1-OE2, and OsNLA1-OE3) accumulated the least levels of Pi...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28626006/atairp2-e3-ligase-affects-aba-and-high-salinity-responses-by-stimulating-its-atp1-sdirip1-substrate-turnover
#8
Tae Rin Oh, Jong Hum Kim, Seok Keun Cho, Moon Young Ryu, Seong Wook Yang, Woo Taek Kim
AtAIRP2 is a cytosolic RING-type E3 ubiquitin (Ub) ligase that positively regulates an ABA response in Arabidopsis (Arabidopsis thaliana). Yeast two-hybrid screening using AtAIRP2 as bait identified ATP1 (AtAIRP2 Target Protein 1) as a substrate of AtAIRP2. ATP1 was found to be identical to SDIRIP1, which was recently reported to be a negative factor in ABA signaling and a target protein of the RING E3 ligase SDIR1. ATP1 was accordingly renamed ATP1/SDIRIP1. A specific interaction between AtAIRP2 and ATP1/SDIRIP1 and ubiquitination of ATP1/SDIRIP1 by AtAIRP2 were demonstrated in vitro and in planta...
June 16, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28623579/yeast-one-and-two-hybrid-high-throughput-screenings-using-arrayed-libraries
#9
Rocío Sánchez-Montesino, Luis Oñate-Sánchez
Since their original description more than 25 years ago, the yeast one- and two-hybrid systems (Y1H/Y2H) have been used by many laboratories to detect DNA-protein (Y1H) and protein-protein interactions (Y2H). These systems use yeast cells (Saccharomyces cerevisiae) as a eukaryotic "test tube" and are amenable for most labs in the world. The development of highly efficient cloning methods has fostered the generation of large collections of open reading frames (ORFs) for several organisms that have been used for yeast screenings...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28623232/the-ring-finger-and-pdz-domain-containing-protein-pdzrn3-controls-localization-of-the-mg-2-regulator-claudin-16-in-renal-tube-epithelial-cells
#10
Kana Marunaka, Chisa Furukawa, Naoko Fujii, Toru Kimura, Takumi Furuta, Toshiyuki Matsunaga, Satoshi Endo, Hajime Hasegawa, Naohiko Anzai, Yasuhiro Yamazaki, Masahiko Yamaguchi, Akira Ikari
Ion exchange in the renal tubules is fundamental to the maintenance of physiological ion levels. Claudin-16 (CLDN16) regulates the paracellular reabsorption of Mg(2+) in the thick ascending limb of Henle's loop in the kidney, with dephosphorylation of CLDN16 increasing its intracellular distribution and decreasing paracellular Mg(2+) permeability. CLDN16 is located in the tight junctions, but the mechanism regulating its localization is unclear. Using yeast two-hybrid systems, we found that CLDN16 binds to PDZRN3, a protein containing both RING-finger and PDZ-domains...
June 16, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28623136/arabidopsis-inositol-polyphosphate-kinase-atipk2%C3%AE-is-phosphorylated-by-cpk4-and-positively-modulates-aba-signaling
#11
Peng Wang, Qiaofeng Yang, Sihong Sang, Yao Chen, Yujiao Zhong, Zhaoyun Wei
Arabidopsis inositol polyphosphate kinase 2β (AtIpk2β) has multiple functions in plant development and in responding to abiotic stress. Although some related clues suggested a potential role of AtIpk2β in ABA signaling, the defined evidence was still lack. Here we discovered that a key ABA signaling component calcium-dependent protein kinase 4 (CPK4) can interact with AtIpk2β under ABA treated conditions through affinity purification and mass spectrometry detection. The interaction between CPK4 and AtIpk2β were further confirmed by yeast two hybrid and bimolecular fluorescence complementation assays...
June 13, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28620080/the-sorting-nexin-3-retromer-pathway-regulates-the-cell-surface-localization-and-activity-of-a-wnt-activated-polycystin-channel-complex
#12
Shuang Feng, Andrew J Streets, Vasyl Nesin, Uyen Tran, Hongguang Nie, Marta Onopiuk, Oliver Wessely, Leonidas Tsiokas, Albert C M Ong
Autosomal dominant polycystic kidney disease (ADPKD) is caused by inactivating mutations in PKD1 (85%) or PKD2 (15%). The ADPKD proteins encoded by these genes, polycystin-1 (PC1) and polycystin-2 (PC2), form a plasma membrane receptor-ion channel complex. However, the mechanisms controlling the subcellular localization of PC1 and PC2 are poorly understood. Here, we investigated the involvement of the retromer complex, an ancient protein module initially discovered in yeast that regulates the retrieval, sorting, and retrograde transport of membrane receptors...
June 15, 2017: Journal of the American Society of Nephrology: JASN
https://www.readbyqxmd.com/read/28615442/the-heterotrimeric-g-protein-g%C3%AE-1-interacts-with-the-catalytic-subunit-of-protein-phosphatase-1-and-modulates-g-protein-coupled-receptor-signaling-in-platelets
#13
Subhashree Pradhan, Tanvir Khatlani, Angus C Nairn, K Vinod Vijayan
Thrombosis is caused by the activation of platelets at the site of ruptured atherosclerotic plaques. This activation involves engagement of G protein coupled receptors (GPCR) on platelets that promotes their aggregation. Although it is known that protein kinases and phosphatases modulate GPCR signaling, how serine/threonine phosphatases integrate with G protein signaling pathways is less understood. Since the subcellular localization and substrate specificity of the catalytic subunit of protein phosphatase 1 (PP1c) is dictated by PP1c-interacting proteins, here we sought to identify new PP1c interactors...
June 14, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28613140/influenza-virus-protein-pb1-f2-interacts-with-calcoco2-ndp52-to-modulate-innate-immune-response
#14
Olivier Leymarie, Léa Meyer, Lionel Tafforeau, Vincent Lotteau, Bruno Da Costa, Bernard Delmas, Christophe Chevalier, Ronan Le Goffic
PB1-F2 is a viral protein encoded by influenza A viruses (IAVs). PB1-F2 is implicated in virulence by triggering immune cell apoptosis and enhancing inflammation. To obtain an insight into the molecular mechanisms of PB1-F2-mediated virulence, we used the yeast two-hybrid approach to find new PB1-F2 cellular interactors. This allowed us to identify calcium-binding and coiled-coil domain 2 (CALCOCO2, also known as NDP52) as a binding partner of PB1-F2. Binding of PB1-F2 to CALCOCO2 was confirmed by pull-down...
June 14, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28603081/pattern-of-expression-and-interaction-specificity-of-multiple-g-protein-beta-g%C3%AE-subunit-isoforms-with-their-potential-target-proteins-reveal-functional-dominance-of-bjug%C3%AE-1-in-the-allotetraploid-brassica-juncea
#15
Roshan Kumar, Aprajita Sharma, Ishita Chandel, Naveen C Bisht
Heterotrimeric G-protein, consisting Gα, Gβ and Gγ subunits, interacts with various upstream and downstream effector (target) proteins to regulate a large array of conserved and species-specific biological functions. The targets of G-protein components are recently reported in model plant Arabidopsis thaliana; however limited information is available from crop species. In this study, we utilized yeast two-hybrid (Y2H) assay to screen the diversity of interacting partners of multiple Gβ subunit isoforms from allotetraploid Brassica juncea, a globally important oilseed and vegetable crop...
May 31, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28597972/a-novel-interacting-protein-serp1-regulates-the-n-linked-glycosylation-and-function-of-glp-1-receptor-in-the-liver
#16
Yuanyuan Xiao, Junfeng Han, Qianqian Wang, Yueqin Mao, Meilin Wei, Weiping Jia, Li Wei
Glucagon-like peptide 1 (GLP-1) exerts multiple effects on metabolism through its receptor, GLP-1R, in the liver. Activation and transduction of GLP-1R require complex interactions of largely unknown accessory proteins, and these processes are crucial to the response to endoplasmic reticulum (ER) stress. Using the membrane-based split ubiquitin yeast two-hybrid system (MYTH) and a human liver cDNA library, we obtained the human GLP-1R interactome and identified SERP1 as a potential interacting protein based on its ability to stabilize membrane proteins and facilitate N-linked glycosylation...
June 9, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28596477/modulation-of-estrogen-related-receptor-alpha-activity-by-the-kinesin-kif17
#17
A M P B Seneviratne, Zeynep Turan, Aurelie Hermant, Patrick Lecine, William O Smith, Jean-Paul Borg, Fanny Jaulin, Geri Kreitzer
Estrogen-related receptor alpha (ERR1) is an orphan nuclear receptor that can bind transcriptional co-activators constitutively. ERR1 expression correlates with poor patient outcomes in breast cancer, heightening interest in this nuclear receptor as a therapeutic target. Because ERR1 has no known regulatory ligand, a major challenge in targeting its activity is to find cellular or synthetic modulators of its function. We identified an interaction between ERR1 and KIF17, a kinesin-2 family microtubule motor, in a yeast-2-hybrid screen...
May 23, 2017: Oncotarget
https://www.readbyqxmd.com/read/28592521/no-evidence-for-extrinsic-post-zygotic-isolation-in-a-wild-saccharomyces-yeast-system
#18
Guillaume Charron, Christian R Landry
Although microorganisms account for the largest fraction of Earth's biodiversity, we know little about how their reproductive barriers evolve. Sexual microorganisms such as Saccharomyces yeasts rapidly develop strong intrinsic post-zygotic isolation, but the role of extrinsic isolation in the early speciation process remains to be investigated. We measured the growth of F1 hybrids between two incipient species of Saccharomyces paradoxus to assess the presence of extrinsic post-zygotic isolation across 32 environments...
June 2017: Biology Letters
https://www.readbyqxmd.com/read/28592505/genome-diversity-and-evolution-in-the-budding-yeasts-saccharomycotina
#19
Bernard A Dujon, Edward J Louis
Considerable progress in our understanding of yeast genomes and their evolution has been made over the last decade with the sequencing, analysis, and comparisons of numerous species, strains, or isolates of diverse origins. The role played by yeasts in natural environments as well as in artificial manufactures, combined with the importance of some species as model experimental systems sustained this effort. At the same time, their enormous evolutionary diversity (there are yeast species in every subphylum of Dikarya) sparked curiosity but necessitated further efforts to obtain appropriate reference genomes...
June 2017: Genetics
https://www.readbyqxmd.com/read/28589840/characterization-of-a-sea-urchin-iq-motif-containing-protein-d-as-a-coactivator-of-nuclear-receptors
#20
Mi Ae Kim, Young Chang Sohn
Nuclear receptor (NR) interacting proteins, such as coactivators and corepressors, play a crucial role in specifying the transcriptional activity of the receptor. However, little is known about the functional features of the NR coregulators in marine invertebrates. Using the yeast two-hybrid screening method, a sea urchin oocyte cDNA library was screened for proteins that interact with the ligand-binding domain of human RXRα (hRXRα) as the bait protein in the presence of 9-cis retinoic acid. Here, we describe IQ motif containing protein D (IQCD) as an RXR-interacting coactivator...
June 2017: Zoological Science
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