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https://www.readbyqxmd.com/read/27913740/a-%C3%AE-ketoacyl-coa-synthase-is-involved-in-rice-leaf-cuticular-wax-synthesis-and-requires-a-cer2-like-protein-as-a-cofactor
#1
Xiaochen Wang, Yuanyuan Guan, Du Zhang, Xiangbai Dong, Lihong Tian, Le Qing Qu
Cuticular waxes are complex mixtures of very-long-chain fatty acids (VLCFAs) and their derivatives, forming a natural barrier on aerial surfaces of terrestrial plants against biotic and abiotic stresses. In VLCFA biosynthesis, β-ketoacyl-CoA synthase (KCS) is the key enzyme, catalyzing the first reaction in fatty acid elongation and determining substrate specificity. We isolated a rice (Oryza sativa L.) wax crystal-sparse leaf 4 (WSL4) gene using a map-based cloning strategy. WSL4 is predicted to encode a KCS, a homolog of Arabidopsis CER6...
December 2, 2016: Plant Physiology
https://www.readbyqxmd.com/read/27913638/ms23-a-master-basic-helix-loop-helix-factor-regulates-the-specification-and-development-of-tapetum-in-maize
#2
Guo-Ling Nan, Jixian Zhai, Siwaret Arikit, Darren Morrow, John Fernandes, Lan Mai, Nhi Nguyen, Blake C Meyers, Virginia Walbot
Successful male gametogenesis involves orchestration of sequential gene regulation for somatic differentiation in pre-meiotic anthers. We report here the cloning of Male Sterile23 (Ms23), encoding an anther-specific predicted basic helix-loop-helix (bHLH) transcription factor required for tapetal differentiation; transcripts localize initially to the precursor secondary parietal cells then predominantly to daughter tapetal cells. In knockout ms23-ref mutant anthers, five instead of the normal four wall layers are observed...
December 2, 2016: Development
https://www.readbyqxmd.com/read/27906451/proximity-dependent-biotinylation-for-identification-of-interacting-proteins
#3
Valerie Le Sage, Alessandro Cinti, Andrew J Mouland
Complex interaction networks orchestrate key cellular processes including but not limited to transcription, translation, metabolism, and cell signaling. Delineating these interactions will aid in deciphering the regulation and function of these pathways and potential for manipulation. Proximity-dependent biotin identification (BioID) is quickly gaining popularity as a powerful tool for identifying novel protein-protein and proximity-based interactions in live cells. This technique relies on a promiscuous biotin ligase, which is fused to a protein of interest and, upon expression in the desired cell, will biotinylate proximal endogenous proteins...
December 1, 2016: Current Protocols in Cell Biology
https://www.readbyqxmd.com/read/27899535/a-tomato-universal-stress-protein-involved-in-oxidative-stress-is-a-cipk6-phosphorylation-target
#4
Emilio Gutierrez-Beltran, José María Personat, Fernando N de la Torre, Olga Del Pozo
Calcineurin B-like interacting protein kinases (CIPKs) decode calcium signals upon interaction with the calcium sensors calcineurin B like proteins (CBLs) into phosphorylation events that result into adaptation to environmental stresses. Few phosphorylation targets of CIPKs are known and therefore the molecular mechanisms underlying their downstream output responses are not fully understood. Tomato (Solanum lycopersicum) Cipk6 regulates immune and susceptible Programmed cell death (PCD) in immunity transforming Ca2+ signals into Reactive Oxygen Species (ROS) signaling...
November 29, 2016: Plant Physiology
https://www.readbyqxmd.com/read/27899274/systematic-identification-of-hepatitis-e-virus-orf2-interactome-reveals-that-tmem134-engages-in-orf2-mediated-nf-%C3%AE%C2%BAb-pathway
#5
Yabin Tian, Weijin Huang, Jun Yang, Zhiheng Wen, Yansheng Geng, Chenyan Zhao, Heqiu Zhang, Youchun Wang
Hepatitis E virus (HEV) is the causative agent of acute hepatitis E. Open reading frame 2 (ORF2) encodes the capsid protein of HEV, which is the main structural protein and may participate, together with the host factors, in viral entry and egress. However, it is still not clear which host proteins are involved in the interaction with ORF2 and what the functions of these ORF2-interacting proteins are. In this study, we have applied a split-ubiquitin yeast two-hybrid screening approach in combination with the pull-down and coimmunoprecipitation assays, identified and validated multiple interacting partners of ORF2 of genotype 1 and 4, which have diverse biological functions...
November 26, 2016: Virus Research
https://www.readbyqxmd.com/read/27898696/regulation-of-gonad-morphogenesis-in-drosophila-melanogaster-by-btb-family-transcription-factors
#6
Diane Silva, Kenneth W Olsen, Magdalena N Bednarz, Andrew Droste, Christopher P Lenkeit, Edwin Chaharbakhshi, Emily R Temple-Wood, Jennifer C Jemc
During embryogenesis, primordial germ cells (PGCs) and somatic gonadal precursor cells (SGPs) migrate and coalesce to form the early gonad. A failure of the PGCs and SGPs to form a gonad with the proper architecture not only affects germ cell development, but can also lead to infertility. Therefore, it is critical to identify the molecular mechanisms that function within both the PGCs and SGPs to promote gonad morphogenesis. We have characterized the phenotypes of two genes, longitudinals lacking (lola) and ribbon (rib), that are required for the coalescence and compaction of the embryonic gonad in Drosophila melanogaster...
2016: PloS One
https://www.readbyqxmd.com/read/27898286/a-conserved-puccinia-striiformis-protein-interacts-with-wheat-npr1-and-reduces-induction-of-pathogenesis-related-genes-in-response-to-pathogens
#7
Xiaodong Wang, Baoju Yang, Kun Li, Zhensheng Kang, Dario Cantu, Jorge Dubcovsky
In Arabidopsis, NPR1 is a key transcriptional co-regulator of systemic acquired resistance. Upon pathogen challenge, NPR1 translocates from the cytoplasm to the nucleus, where it interacts with TGA-bZIP transcription factors to activate the expression of several Pathogenesis-Related (PR) genes. In a screen of a yeast two-hybrid library from wheat leaves infected with Puccinia striiformis f. sp. tritici, we identified a conserved rust protein that interacts with wheat NPR1 and named it Puccinia NPR1 interactor (PNPi)...
November 29, 2016: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/27897225/calmodulin-interacts-with-rab3d-and-modulates-osteoclastic-bone-resorption
#8
Sipin Zhu, Shek Man Chim, Taksum Cheng, Estabelle Ang, Benjamin Ng, Baysie Lim, Kai Chen, Heng Qiu, Jennifer Tickner, Huazi Xu, Nathan Pavlos, Jiake Xu
Calmodulin is a highly versatile protein that regulates intracellular calcium homeostasis and is involved in a variety of cellular functions including cardiac excitability, synaptic plasticity and signaling transduction. During osteoclastic bone resorption, calmodulin has been reported to concentrate at the ruffled border membrane of osteoclasts where it is thought to modulate bone resorption activity in response to calcium. Here we report an interaction between calmodulin and Rab3D, a small exocytic GTPase and established regulator osteoclastic bone resorption...
November 29, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27896439/characterization-of-pollen-expressed-bzip-protein-interactions-and-the-role-of-atbzip18-in-the-male-gametophyte
#9
Antónia Gibalová, Lenka Steinbachová, Said Hafidh, Veronika Bláhová, Zuzana Gadiou, Christos Michailidis, Karel Műller, Roman Pleskot, Nikoleta Dupľáková, David Honys
KEY MESSAGE : bZIP TF network in pollen. Transcriptional control of gene expression represents an important mechanism guiding organisms through developmental processes and providing plasticity towards environmental stimuli. Because of their sessile nature, plants require effective gene regulation for rapid response to variation in environmental and developmental conditions. Transcription factors (TFs) provide such control ensuring correct gene expression in spatial and temporal manner. Our work reports the interaction network of six bZIP TFs expressed in Arabidopsis thaliana pollen and highlights the potential functional role for AtbZIP18 in pollen...
November 28, 2016: Plant Reproduction
https://www.readbyqxmd.com/read/27895204/two-h-type-thioredoxins-interact-with-the-pho2ubiquitin-conjugating-e2-enzyme-to-fine-tune-phosphate-homeostasis
#10
Yinghui Ying, Wenhao Yue, Shoudong Wang, Shuai Li, Min Wang, Yang Zhao, Chuang Wang, Chuanzao Mao, James Whelan, Huixia Shou
Phosphate over-accumulator 2 (PHO2) encodes an ubiquitin-conjugating E2 enzyme that is a major negative regulator of the inorganic phosphate (Pi)-starvation response-signaling pathway. A yeast-two-hybrid (Y2H) screen in Oryza sativa (Os, rice) using OsPHO2 as bait, revealed an interaction between OsPHO2 and two h-type thioredoxins, OsTrxh1 and OsTrxh4. These interactions were confirmed in vivo using bimolecular fluorescence complementation (BiFC) of OsPHO2 and OsTrxh1/h4 in rice protoplasts, and by in vitro pull-down assays with 6His-tagged OsTrxh1/h4 and GST-tagged OsPHO2...
November 28, 2016: Plant Physiology
https://www.readbyqxmd.com/read/27888470/mutation-of-the-ser18-phosphorylation-site-on-the-sole-saccharomyces-cerevisiae-ucs-protein-she4-can-compromise-high-temperature-survival
#11
Susana Gomez-Escalante, Peter W Piper, Stefan H Millson
Folding of the myosin head often requires the joint actions of Hsp90 and a dedicated UNC45, Cro1, She4 (UCS) domain-containing cochaperone protein. Relatively weak sequence conservation exists between the single UCS protein of simple eukaryotes (She4 in budding yeast) and the two UCS proteins of higher organisms (the general cell and smooth muscle UNC45s; UNC45-GC and UNC45-SM respectively). In vertebrates, UNC45-GC facilitates cytoskeletal function whereas the 55% identical UNC45-SM assists in the assembly of the contractile apparatus of cardiac and skeletal muscles...
November 25, 2016: Cell Stress & Chaperones
https://www.readbyqxmd.com/read/27882103/identification-of-proteins-suppressing-the-functions-of-oncogenic-phosphatase-of-regenerating-liver-1-and-3
#12
Ju-Dong Lee, Haiyoung Jung, Sang-Hyun Min
The phosphatase of regenerating liver (PRL) family, including PRL-1, PRL-2, and PRL-3, comprises protein tyrosine phosphatases whose deregulation is associated with the tumorigenesis and metastasis of many types of cancer. However, the underlying mechanism is poorly understood. In this study, aiming to increase understanding of the molecular mechanisms underlying the functions of PRL-1 and PRL-3, a yeast two-hybrid system was employed to screen for their interacting proteins. Alignment with the NCBI BLAST database revealed 12 interactive proteins: Synaptic nuclear envelope protein 2, emerin, mannose 6-phosphate receptor-binding protein 1, low-density lipoprotein receptor-related protein 10, Rab acceptor 1, tumor protein D52-like 2, selectin P ligand (SELPLG), guanylate binding protein 1, transmembrane and ubiquitin-like domain-containing 2, NADH:ubiquinone oxidoreductase subunit B8, syndecan 4 and FK506-binding protein 8 (FKBP8)...
November 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/27874987/retraction
#13
(no author information available yet)
This article corrects: Retracted: Three BUB1 and BUBR1/MAD3-related spindle assembly checkpoint proteins are required for accurate mitosis in Arabidopsis. New Phytologist 205: 202-215. Article first published online: 29 September 2014. This article has been retracted at the request of: Editor-in-Chief and Author 'Three BUB1 and BUBR1/MAD3-related spindle assembly checkpoint proteins are required for accurate mitosis in Arabidopsis', by Paganelli L, Caillaud M-C, Quentin M, Damiani I, Govetto B, Lecomte P, Karpov, PA, Abad P, Chabouté M-E and Favery B...
December 2016: New Phytologist
https://www.readbyqxmd.com/read/27873207/profiling-phosphopeptide-binding-domain-recognition-specificity-using-peptide-microarrays
#14
Michele Tinti, Simona Panni, Gianni Cesareni
Cellular organization and response to internal and external stimuli are mediated by an intricate web of protein interactions. Some of these interactions are regulated by covalent posttranslational modifications such as phosphorylation and acetylation. These modifications can change the chemical nature of the interaction interfaces and modulate the binding affinity of the interacting partners. In signal transduction, the most frequent modification is reversible phosphorylation of tyrosine, serine or threonine residues...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27872659/whole-genome-de-novo-sequencing-combined-with-rna-seq-analysis-reveals-unique-genome-and-physiological-features-of-the-amylolytic-yeast-saccharomycopsis-fibuligera-and-its-interspecies-hybrid
#15
Jin Ho Choo, Chang Pyo Hong, Jae Yun Lim, Jeong-Ah Seo, Young-Suk Kim, Dong Wook Lee, Sin-Gi Park, Gir Won Lee, Emily Carroll, Yin-Won Lee, Hyun Ah Kang
BACKGROUND: Genomic studies on fungal species with hydrolytic activity have gained increased attention due to their great biotechnological potential for biomass-based biofuel production. The amylolytic yeast Saccharomycopsis fibuligera has served as a good source of enzymes and genes involved in saccharification. Despite its long history of use in food fermentation and bioethanol production, very little is known about the basic physiology and genomic features of S. fibuligera. RESULTS: We performed whole-genome (WG) de novo sequencing and complete assembly of S...
2016: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/27866222/the-cytoskeletal-protein-septin-11-is-associated-with-human-obesity-and-is-involved-in-adipocyte-lipid-storage-and-metabolism
#16
Natalia Moreno-Castellanos, Amaia Rodríguez, Yoana Rabanal-Ruiz, Alejandro Fernández-Vega, José López-Miranda, Rafael Vázquez-Martínez, Gema Frühbeck, María M Malagón
AIMS/HYPOTHESIS: Septins are newly identified members of the cytoskeleton that have been proposed as biomarkers of a number of diseases. However, septins have not been characterised in adipose tissue and their relationship with obesity and insulin resistance remains unknown. Herein, we characterised a member of this family, septin 11 (SEPT11), in human adipose tissue and analysed its potential involvement in the regulation of adipocyte metabolism. METHODS: Gene and protein expression levels of SEPT11 were analysed in human adipose tissue...
November 19, 2016: Diabetologia
https://www.readbyqxmd.com/read/27866166/variability-in-a-short-tandem-repeat-mediates-complex-epistatic-interactions-in-arabidopsis-thaliana
#17
Maximilian Oliver Press, Christine Queitsch
Short tandem repeats are hypervariable genetic elements that occur frequently in coding regions. Their high mutation rate readily generates genetic variation contributing to adaptive evolution and human diseases. We previously reported that natural ELF3 polyglutamine variants cause reciprocal genetic incompatibilities in two divergent Arabidopsis thaliana backgrounds. Here, we dissect the genetic architecture of this incompatibility, revealing as many as four loci putatively interacting with ELF3 We were able to specifically identify one such ELF3-interacting gene, LSH9 We further used a yeast two-hybrid strategy to identify proteins whose physical interactions with ELF3 were affected by polyglutamine tract length...
November 18, 2016: Genetics
https://www.readbyqxmd.com/read/27864322/e-coli-proteome-microarrays-identified-the-substrates-of-clpyq-protease
#18
Chih-Hsuan Tsai, Yu-Hsuan Ho, Tzu-Cheng Sung, Whei-Fen Wu, Chien-Sheng Chen
Proteolysis is a vital mechanism to regulate the cellular proteome in all kingdoms of life, and ATP-dependent proteases play a crucial role within this process. In Escherichia coli, ClpYQ is one of the primary ATP-dependent proteases. In addition to function with removals of abnormal peptides in the cells, ClpYQ degrades regulatory proteins if necessary and thus let cells adjust to various environmental conditions. In E. coli, SulA, RcsA, RpoH and TraJ as well as RNase R, have been identified as natural protein substrates of ClpYQ...
November 18, 2016: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/27861562/digitor-dasciz-has-multiple-roles-in-drosophila-development
#19
Saheli Sengupta, Uttama Rath, Changfu Yao, Michael Zavortink, Chao Wang, Jack Girton, Kristen M Johansen, Jørgen Johansen
In this study we provide evidence that the spindle matrix protein Skeletor in Drosophila interacts with the human ASCIZ (also known as ATMIN and ZNF822) ortholog, Digitor/dASCIZ. This interaction was first detected in a yeast two-hybrid screen and subsequently confirmed by pull-down assays. We also confirm a previously documented function of Digitor/dASCIZ as a regulator of Dynein light chain/Cut up expression. Using transgenic expression of a mCitrine-labeled Digitor construct, we show that Digitor/dASCIZ is a nuclear protein that is localized to interband and developmental puff chromosomal regions during interphase but redistributes to the spindle region during mitosis...
2016: PloS One
https://www.readbyqxmd.com/read/27860167/jrwrky2-and-jrwrky7-of-juglans-regia-mediate-responses-to-abiotic-stresses-and-abscisic-acid-by-formation-of-homodimers-and-interaction
#20
Guiyan Yang, Wenhui Zhang, Zexing Liu, A-Yijiamali Yi-Maer, Meizhi Zhai, Zhenggang Xu
[Backgroud] WRKY transcription factors (TFs) belong to a large protein family that is involved in diverse developmental processes and abiotic stress responses. Currently, there is little understanding of the role of WRKY TFs in regulatory mechanisms in plants, especially in the protein-protein interactions that are essential for biological regulatory functions and networks. [Methods] In the present study, the yeast one-hybrid, yeast two-hybrid, transient expression and quantitative RT-PCR were applied to investigate the potential characters of two WRKY proteins from Juglans regia, JrWRKY2 (GenBank No...
November 11, 2016: Plant Biology
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