Ganeko Bernardo-Seisdedos, Jon Bilbao, David Fernández-Ramos, Fernando Lopitz-Otsoa, Virginia Gutierrez de Juan, Maider Bizkarguenaga, Borja Mateos, Marcos F Fondevila, Jordi Abril-Fornaguera, Tammo Diercks, Shelly C Lu, Rubén Nogueiras, José M Mato, Oscar Millet
The liver plays a central role in all metabolic processes in the body. However, precise characterization of liver metabolism is often obscured by its inherent complexity. Phosphorylated metabolites occupy a prominent position in all anabolic and catabolic pathways. Here we develop a 31 P-NMR-based method to study the liver "phosphorome" through the simultaneous identification and quantification of multiple hydrophilic and hydrophobic phosphorylated metabolites. We applied this technique to define the metabolic landscape in livers form a mouse model of the rare disease disorder congenital erythropoietic porphyria (CEP), as well as two well-known murine models of nonalcoholic steatohepatitis, one genetic, methionine adenosyltransferase 1A knockout mice, and the other dietary, mice fed a high-fat choline deficient diet...
December 7, 2020: Hepatology: Official Journal of the American Association for the Study of Liver Diseases