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https://www.readbyqxmd.com/read/28533186/delayed-identification-of-acinetobacter-baumannii-during-an-outbreak-owing-to-disrupted-blaoxa-51-like-by-isaba19
#1
Amjad Ahmadi, Himen Salimizand
Early detection of Acinetobacter baumannii, an emerging pathogen in hospital settings, is of interest. The blaOXA-51-like family had been used as a marker to detect A. baumannii. During an infection outbreak, 14 isolates produced a 1.7-kb PCR band instead of 353 bp for this marker. This work sought the reasons behind the increased marker size. Characterisation of isolates was done by API-20NE, gyrB multiplex PCR and 16S-23S rRNA ITS sequencing. The 1.7-kb band generated and the complete blaOXA-51-like variant were sequenced to find the probable integrated element...
May 19, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28532533/glycaemic-index-and-glycaemic-load-values-of-commonly-consumed-foods-in-the-united-arab-emirates
#2
Ayesha S Al Dhaheri, C Jeyakumar K Henry, Maysm N Mohamad, Eric O Ohuma, Leila Cheikh Ismail, Fatima T Al Meqbaali, Amjad H Jarrar
Glycaemic index (GI) and glycaemic load (GL) values of some commonly consumed foods in the United Arab Emirates were determined with an aim of adding these values to the existing international table of GI and GL values. In all, eighteen test foods categorised into breads (n 5), entrée dishes (n 3), main dishes (n 5) and sweet dishes (n 5) were tested. For each test food, at least fifteen healthy participants consumed 25 or 50 g available carbohydrate portions of a reference food (glucose), which was tested three times, and a test food after an overnight fast, was tested once, on separate occasions...
May 23, 2017: British Journal of Nutrition
https://www.readbyqxmd.com/read/28532009/exploring-recent-developments-to-improve-antioxidant-anti-inflammatory-and-antimicrobial-efficacy-of-curcumin-a-review-of-new-trends-and-future-perspectives
#3
REVIEW
Zahid Hussain, Hnin Ei Thu, Muhammad Wahab Amjad, Fahad Hussain, Tarek A Ahmed, Shahzeb Khan
Curcumin derivatives have been well-documented due to their natural antioxidant, antimicrobial and anti-inflammatory activities. Curcuminoids have also gained widespread recognition due to their wide range of other activities which include anti-infective, anti-mutagenic, anticancer, anti-coagulant, antiarthrititc, and wound healing potential. Despite of having a wide range of activities, the inherent physicochemical characteristics (poor water solubility, low bioavailability, chemical instability, photodegradation, rapid metabolism and short half-life) of curcumin derivatives limit their pharmaceutical significance...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28529810/molecular-profiling-a-case-of-zbtb16-rara-acute-promyelocytic-leukemia
#4
Stephen E Langabeer, Lisa Preston, Johanna Kelly, Matt Goodyer, Ezzat Elhassadi, Amjad Hayat
Several variant RARA translocations have been reported in acute promyelocytic leukemia (APL) of which the t(11;17)(q23;q21), which results in a ZBTB16-RARA fusion, is the most widely identified and is largely resistant to therapy with all-trans retinoic acid (ATRA). The clinical course together with the cytogenetic and molecular characterization of a case of ATRA-unresponsive ZBTB16-RARA APL is described. Additional mutations potentially cooperating with the translocation fusion product in leukemogenesis have been hitherto unreported in ZBTB16-RARA APL and were sought by application of a next-generation sequencing approach to detect those recurrently found in myeloid malignancies...
2017: Case Reports in Hematology
https://www.readbyqxmd.com/read/28526937/molecular-confirmation-of-intraspecific-tomato-solanum-lycopersicum-hybrids-and-their-evaluation-against-late-blight-and-cucumber-mosaic-virus
#5
Amjad Hameed, Muhammad Yussouf Saleem, Khalid Pervaiz Akhtar, Muhammad Shoaib, Qumer Iqbal, Muhammad Asghar
Tomato is one of the most consumed vegetables in the world. Diseases are the number one concern in the development of high-yield and disease-resistant tomato hybrids which is the foremost priority of breeders. Present study was conducted (1) to develop DNA-based markers for genetic confirmation of tomato F1 hybrids, (2) to utilize sequenced characterized amplified region (SCAR) marker linked to the Ph-3 gene for Phytophthora infestans resistance in tomato and (3) to evaluate male and female parental genotypes and their F1 hybrids against late blight (LB) and cucumber mosaic virus (CMV)...
May 19, 2017: Molecular Biotechnology
https://www.readbyqxmd.com/read/28523026/are-we-missing-the-diagnosis-of-depression-in-patients-with-rheumatoid-arthritis-at-a-tertiary-care-facility
#6
Ammara Masood, Babur Salim, Amjad Nasim, Ziaullah Khalid, Amir Afzal
OBJECTIVES: To determine if we are missing clinical depression in patients with Rheumatoid Arthritis and its relationship with functional disability and level of formal education in such patients. METHODS: The data for this cross-sectional, analytical study was gathered from May 2015 till December 2015 and comprised of 128 with Rheumatoid arthritis diagnosed according to ACR/EULAR 2010 criteria. The study was conducted at Fauji Foundation Hospital Rawalpindi. Functional status was assessed with Modified Health Assessment Questionnaire (mHAQ) and Beck's Depression Inventory (BDI) was used for evaluation of symptoms of depression...
March 2017: Pakistan Journal of Medical Sciences Quarterly
https://www.readbyqxmd.com/read/28511952/lithium-ameliorates-lipopolysaccharide-induced-neurotoxicity-in-the-cortex-and-hippocampus-of-the-adult-rat-brain
#7
Muhammad Sohail Khan, Tahir Ali, Muhammad Noman Abid, Myeung Hoon Jo, Amjad Khan, Min Woo Kim, Gwang Ho Yoon, Eun Woo Cheon, Shafiq Ur Rehman, Myeong Ok Kim
Lithium an effective mood stabilizer, primary used in the treatment of bipolar disorders, has been reported as a protective agent in various neurological disorders. In this study, we examined the neuroprotective role of lithium chloride (LiCl) against lipopolysaccharide (LPS) in the cortex and hippocampus of the adult rat brain. We determined that LiCl -attenuated LPS-induced activated toll-like receptor 4 (TLR4) signalling and significantly reduced the nuclear factor-kB (NF-KB) translation factor and various other inflammatory mediators such as interleukin-1 beta (IL-1β) and tumour necrosis factor alpha (TNF-α)...
May 13, 2017: Neurochemistry International
https://www.readbyqxmd.com/read/28507476/effect-of-clopidogrel-on-the-hydroxylation-and-sulfoxidation-of-omeprazole-a-single-dose-study-in-healthy-human-volunteers
#8
Lateef Ahmad, Zafar Iqbal, Shabnam Nazir, Abad Khan, Yasar Shah, Muhammad Imran Khan, Ismail Khan, Amjad Khan
Based upon the known potential interaction between omeprazole (OMP) and clopidogrel (CLOP), the current study was designed to evaluate the effect of CLOP on disposition of OMP and its two major metabolites, 5-hydroxyomeprazole (5-OH-OMP) and omeprazole sulfone (OMP-S) in healthy clinical subjects. A randomized, open label, 2-period, crossover study was designed. Twelve volunteers were selected, of whom eight were extensive metabolizers (EM) of CYP2C19 and 4 were poor metabolizers (PM). They received single dose of OMP either alone or in combination with CLOP (single dose) and samples were collected periodically to calculate various pharmacokinetic parameters...
2017: EXCLI journal
https://www.readbyqxmd.com/read/28507373/comparison-of-low-level-laser-therapy-and-interferential-current-on-post-stroke-shoulder-pain
#9
Fahmida Jan, Aamir Naeem, Arshad Nawaz Malik, Imran Amjad, Tariq Malik
The objective of the study was to compare the effects of LASER therapy and Interferential current on post stroke shoulder pain. Diagnosed patients (n=38) of post stroke shoulder pain were randomly divided into two groups i.e., LASER group (LG =20 patients) and Interferential current group (IFCG=18 patients). The variables under study were pain, satisfaction, disability, and function level before and after treatment. Improvement in pain and satisfaction level after LASER therapy was significant (p<0.001)...
May 2017: JPMA. the Journal of the Pakistan Medical Association
https://www.readbyqxmd.com/read/28506769/molecular-biomarkers-for-an-early-diagnosis-effective-treatment-and-prognosis-of-colorectal-cancer-current-updates
#10
REVIEW
Amjad Mahasneh, Fawaz Al-Shaheri, Eshraq Jamal
Colorectal cancer (CRC) is the third most prevalent cancer in the world. Globally, it has been estimated that about 1.4 million new cases of colorectal cancer are diagnosed every year. CRC is a multifactorial disease that arises due to genetics as well as epigenetic alterations in a number of oncogenes, tumor suppressor genes, mismatch repair genes, as well as cell cycle regulating genes in colon mucosal cells. These molecular alterations have been considered as potential CRC biomarkers because they can provide the physicians with diagnostic, prognostic and treatment response information...
May 12, 2017: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/28506259/detection-of-antibacterial-activities-of-miswak-kalonji-and-aloe-vera-against-oral-pathogens-anti-proliferative-activity-against-cancer-cell-line
#11
Sameen Amjed, Kashaf Junaid, Junaid Jafar, Tuaha Amjad, Waqas Maqsood, Nadia Mukhtar, Kanza Tariq, Musarrat Sharif, Sana Javaid Awan, Farheen Ansari
BACKGROUND: Emerging drug resistance and hindrance of treatment is provoking scientists to search new, less expensive medicinally active compounds. Dental diseases caused by oral pathogens are very frequent chronic infections around the world. The medical potentials of a lot of Pakistani local herbs and herbal combinations is relatively unknown, hence attempted to explore. A study was designed to investigate potential role of local medicinal herbs for example Miswak, Kalonji & Aloe vera as antimicrobial, antioxidant and anti-proliferative agents against oral pathogens and cancer cell line...
May 15, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28504166/functionality-of-chitosan-in-batter-formulations-for-coating-of-fish-sticks-effect-on-physicochemical-quality
#12
K A Martin Xavier, Hauzoukim, Nagalakshmi Kannuchamy, Amjad K Balange, M K Chouksey, Venkateshwarlu Gudipati
Chitosan is a natural polymer having diverse applications in food industry. The present study was undertaken to evaluate chitosan as a hydrocolloid in batter composition in developing enrobed fish sticks with better functional properties and improved quality. Different concentrations of chitosan gel were made in acetic acid medium and incorporated into batter for making enrobed fish sticks. Coating parameters, fat uptake, oil reduction, chemical quality parameters, instrumental texture analysis and colour were studied...
August 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28501582/molecular-detection-of-rifampicin-resistance-by-genexpert-%C3%A2-assay-among-treated-and-untreated-pulmonary-tuberculosis-patients-from-khyber-pakhtunkhwa-pakistan
#13
Hazir Rahman, Shakir Ullah Khan, Muhamamd Amjad Khan, Muhammad Qasim, Abdul Jabbar, Sadiq Noor, Zakir Khan, Taj Ali Khan, Mubbashir Hussain, Noor Muhammad, Nawab Ali
OBJECTIVES: Drug-resistant tuberculosis (TB) is an emerging health problem. Rifampicin (RIF) is the major first-line drug against TB. RIF resistance can be used as a marker for the detection of multidrug-resistant TB (MDR-TB). The purpose of this study was to determine the RIF resistance pattern of Mycobacterium tuberculosis complex isolates among treated and untreated patients in Khyber Pakhtunkhwa, Pakistan. METHODS: A total of 349 drug-treated and untreated TB-diagnosed patients were enrolled in this study...
May 10, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28494613/new-natural-product-an-efficient-antimicrobial-applications-of-new-newly-synthesized-pyrimidine-derivatives-by-the-electrochemical-oxidation-of-hydroxyl-phenol-in-the-presence-of-2-mercapto-6-trifluoromethyl-pyrimidine-4-ol-as-nucleophile
#14
Zia Ul Haq Khan, Amjad Khan, Pingyu Wan, Arif Ullah Khan, Kamran Tahir, Nawshad Muhammad, Faheem Ullah Khan, Hidayat Ullah Shah, Zia Ullah Khan
Some new pyrimidine derivatives have been synthesised by electrochemical oxidation of catechol (1a) in the existence of 2-mercapto-6-(trifluoromethyl) pyrimidine-4-ol (3) as a nucleophile in aqueous solution using Cyclic Voltammetric and Controlled Potential Coulometry. The catechol has been oxidised to o-quinone through electrochemical method and participative in Michael addition reaction, leading to the development of some new pyrimidine derivatives. The products were achieved in good yield with high pureness...
May 11, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28492160/very-late-stent-thrombosis-is-there-any-limit-when-to-stop-antiplatelet-therapy
#15
Muhammad Nasir Rahman, Ibrahim Gul, Amjad Nabi
No abstract text is available yet for this article.
April 2017: Journal of the College of Physicians and Surgeons—Pakistan: JCPSP
https://www.readbyqxmd.com/read/28487172/prediction-of-vaccine-candidates-against-pseudomonas-aeruginosa-an-integrated-genomics-and-proteomics-approach
#16
Muhammad Ibrahim Rashid, Anam Naz, Amjad Ali, Saadia Andleeb
Pseudomonas aeruginosa is among top critical nosocomial infectious agents due to its persistent infections and tendency for acquiring drug resistance mechanisms. To date, there is no vaccine available for this pathogen. We attempted to exploit the genomic and proteomic information of P. aeruginosa though reverse-vaccinology approaches to unveil the prospective vaccine candidates. P. aeruginosa strain PAO1 genome was subjected to sequential prioritization approach following genomic, proteomics and structural analyses...
May 6, 2017: Genomics
https://www.readbyqxmd.com/read/28486870/long-term-cardiac-morbidity-and-mortality-in-patients-with-aortic-valve-disease-following-liver-transplantation-a-case-matching-study
#17
Ramona Nicolau-Raducu, Thomas Marshall, Hamang Patel, Kelly Ural, Joseph Koveleskie, Susan Smith, Donald Ganier, Bryan Evans, Brian Fish, William Daly, Ari J Cohen, George Loss, Amjad Bokhari, Bobby Nossaman
INTRODUCTION: This retrospective study examined the role of aortic valve (AV) disease in patients who underwent orthotopic liver transplantation (OLT) to determine the incidence of postoperative cardiac morbidity and mortality when compared with a matched control group without AV disease. METHODS: Patients were included in the AV group if diagnosed with aortic stenosis (AS) or aortic regurgitation or had received AV replacement prior to OLT. The AV group (n = 53) was matched to a control group (n = 212) with the following preoperative variables: type of organ transplanted, age, gender, race, body mass index, MELD, redo-transplantation, preoperative renal replacement therapy, nonalcoholic steatohepatitis, viral hepatitis, diabetes, and coronary artery disease...
May 1, 2017: Seminars in Cardiothoracic and Vascular Anesthesia
https://www.readbyqxmd.com/read/28484986/validation-of-a-clinical-pathway-to-assess-asymptomatic-renal-transplant-candidates-using-myocardial-perfusion-imaging
#18
Rami Doukky, Ibtihaj Fughhi, Tania Campagnoli, Marwan Wassouf, Michael Kharouta, Aviral Vij, Chiedozie Anokwute, Andrew Appis, Amjad Ali
BACKGROUND: An AHA/ACCF scientific statement proposed 8 risk factors to assess the need for noninvasive coronary artery disease (CAD) surveillance in asymptomatic patients undergoing evaluation for kidney transplantation. The clinical application of these risk factors and the role of noninvasive testing in this context have not been defined. METHODS AND RESULTS: We retrospectively followed a cohort of 581 consecutive kidney transplant recipients of whom 401 had pre-transplant radionuclide myocardial perfusion imaging (MPI) and 90 had pre-transplant coronary angiography...
May 8, 2017: Journal of Nuclear Cardiology: Official Publication of the American Society of Nuclear Cardiology
https://www.readbyqxmd.com/read/28482786/nanotechnology-based-theranostic-approaches-in-alzheimer-s-disease-management-current-status-and-future-perspective
#19
Javed Ahmad, Sohail Akhter, Md Rizwanullah, Mohammad Ahmed Khan, Lucie Pigeon, Richard T Addo, Nigel H Greig, Patrick Midoux, Chantal Pichon, Mohammad Amjad Kamal
Alzheimer's disease (AD), a cognitive dysfunction/dementia state amongst the elders is characterized by irreversible neurodegeneration due to varied pathophysiology. Up till now, anti-AD drugs having different pharmacology have been developed and used in clinic. Yet, these medications are not curative and only lowering the AD associated symptoms. Improvement in treatment outcome required drug targeting across the blood-brain barrier (BBB) to the central nervous system (CNS) in optimal therapeutic concentration...
May 8, 2017: Current Alzheimer Research
https://www.readbyqxmd.com/read/28479723/biosynthesis-characterization-and-biological-activities-of-iron-nanoparticles-using-sesamum-indicum-seeds-extract
#20
Farah Bano, Muhammad Baber, Amjad Ali, Ziaullah Shah, Syed Aun Muhammad
BACKGROUND: Iron nanoparticles (FeNPs) have got many biomedical and health applications because of biocompatible and nontoxic nature to humans. OBJECTIVE: To synthesize the FeNPs using natural sources. MATERIALS AND METHODS: In this study, simple and economical procedure was adopted for FeNPs synthesis. Sesame seeds were processed to obtain seed extract as a biological material for FeNPs production. FeNPs were characterized by Fourier transform infrared spectroscopy, X-ray diffraction, and scanning electron microscopic...
January 2017: Pharmacognosy Magazine
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