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allergen cross-reactive

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https://www.readbyqxmd.com/read/29202918/almond-or-mahaleb-orthogonal-allergen-analysis-during-a-live-incident-investigation-by-elisa-molecular-biology-and-protein-mass-spectrometry
#1
Michael J Walker, Malcolm Burns, Milena Quaglia, Gavin Nixon, Christopher J Hopley, Kirstin M Gray, Victoria Moore, Malvinder Singh, Simon Cowen
It is now well known that an incident investigated in the United Kingdom in 2015 of cumin alleged to be contaminated with almond, a risk for people with almond allergy, was caused by the Prunus species, Prunus mahaleb. In the United Kingdom, the Government Chemist offers a route of technical appeal from official findings in the food control system. Findings of almond in two official samples, cumin and paprika, which had prompted action to exclude the consignments from the food chain, were so referred. Herein are described the approaches deployed to resolve the analytical issues during the investigation of the incidents...
December 5, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/29202912/assessing-almond-and-peanut-allergens-using-commercially-available-immunoanalytical-kits-and-lc-ms-ms-a-case-study
#2
Matthew Daly, Parisa Ansari, Georg Häubl, Adrian Rogers, Kurt Brunner
With an ever-increasing allergic population and an emerging market for allergen-free foods, accurate detectionof allergens in foods has never been more important. Although ELISA-based methods are the most widely used for detection of allergens in food, there is a need for the development of orthogonal approaches. A commercial ELISA detected a relatively high concentration of peanut and almond in an allergen-free product. However, another commercial ELISA declared a low peanut concentration and was negative for almond...
December 5, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/29202909/commercial-elisa-measurement-of-allergens-and-gluten-what-we-can-learn-from-case-studies
#3
Markus Lacorn, Stella Lindeke, Susanne Siebeneicher, Thomas Weiss
During the last decade, results from ELISA test kits have often been criticized as being flawed. Therefore, itmay appear counterintuitive that ELISAs are used for most food allergen and gluten analytical needs. One reason, in addition to the nonavailability of comparable alternative methods, is the fact that the methods used underwent a long validation and learning period in the market. This publication presents several case studies on interference, cross-reactivity issues, calibrators, fragmented allergens and gluten, matrix influences, and misunderstood intended-use statements...
December 5, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/29200762/allergen-sensitization-pattern-by-sex-a-cluster-analysis-in-korea
#4
Jungyoon Ohn, Seung Hwan Paik, Eun Jin Doh, Hyun-Sun Park, Hyun-Sun Yoon, Soyun Cho
Background: Allergens tend to sensitize simultaneously. Etiology of this phenomenon has been suggested to be allergen cross-reactivity or concurrent exposure. However, little is known about specific allergen sensitization patterns. Objective: To investigate the allergen sensitization characteristics according to gender. Methods: Multiple allergen simultaneous test (MAST) is widely used as a screening tool for detecting allergen sensitization in dermatologic clinics...
December 2017: Annals of Dermatology
https://www.readbyqxmd.com/read/29199966/occupational-asthma-and-urticaria-in-a-fishmonger-due-to-creatine-kinase-a-cross-reactive-fish-allergen
#5
X Larco-Rojas, M L González-Gutiérrez, S Vázquez-Cortés, B Bartolomé, C Pastor-Vargas, M Fernández-Rivas
No abstract text is available yet for this article.
2017: Journal of Investigational Allergology & Clinical Immunology
https://www.readbyqxmd.com/read/29178679/detecting-allergens-from-black-tiger-shrimp-penaeus-monodon-that-can-bind-and-cross-link-ige-by-elisa-western-blot-and-a-humanized-rat-basophilic-leukemia-reporter-cell-line-rs-atl8
#6
Thanyapat Jarupalee, Pantipa Chatchatee, Kittinan Komolpis, Narissara Suratannon, Sittiruk Roytrakul, Yodying Yingchutrakul, Wanaporn Yimchuen, Patcharavadee Butta, Alain Jacquet, Tanapat Palaga
BACKGROUND: Black tiger shrimp Penaeus monodon is one of the common causes of shellfish allergy that is increasing worldwide. One of the important problems in the management of shellfish allergy is the lack of accurate diagnostic assay because the biological and immunological properties of allergens in black tiger shrimp have not been well characterized. This study aims to detect proteins with the ability to bind and cross-link immunoglobulin E (IgE) from black tiger shrimp by enzyme-linked immunosorbent assay (ELISA), Western blot, and a humanized rat basophilic leukemia reporter cell line RS-ATL8...
January 2018: Allergy, Asthma & Immunology Research
https://www.readbyqxmd.com/read/29171912/concomitant-sensitization-to-legumin-fag-e-2-and-fag-e-5-predicts-buckwheat-allergy
#7
S Geiselhart, C Nagl, P Dubiela, A C Pedersen, M Bublin, C Radauer, C Bindslev-Jensen, K Hoffmann-Sommergruber, C G Mortz
BACKGROUND: Buckwheat (Fagopyrum esculentum) has become increasingly popular as a healthy food in Europe. However, for sensitized individuals, consumption can cause anaphylactic reactions. The aim of this study was to identify individual well-characterized buckwheat allergens for component resolved diagnosis. METHODS: Patients were selected by positive skin prick test to buckwheat and divided into two groups: (1) sensitized to buckwheat without clinical symptoms and (2) buckwheat allergy...
November 24, 2017: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29171882/characterization-of-epitope-specificities-of-reference-antibodies-used-for-the-quantification-of-the-birch-pollen-allergen-bet-v-1
#8
Sébastien Brier, Maxime Le Mignon, Karine Jain, Constance Lebrun, François Peurois, Christine Kellenberger, Véronique Bordas-Le Floch, Laurent Mascarell, Emmanuel Nony, Philippe Moingeon
BACKGROUND: Accurate allergen quantification is needed to document the consistency of allergen extracts used for immunotherapy. Herein, we characterize the epitope specificities of two monoclonal antibodies used in an ELISA for the quantification of the major birch pollen allergen Bet v 1, established as a reference by the BSP090 European project. METHODS: The ability of mAbs 5B4 and 6H4 to recognize Bet v 1 isoforms was addressed by immunochromatography. The capacity of each mAb to compete with patients' IgE for binding to Bet v 1 was measured by ELISA inhibition...
November 24, 2017: Allergy
https://www.readbyqxmd.com/read/29159565/a-comprehensive-review-on-mustard-induced-allergy-and-implications-for-human-health
#9
REVIEW
Akanksha Sharma, Alok K Verma, Rinkesh Kumar Gupta, Neelabh, Premendra D Dwivedi
Mustard is widely used in a variety of foods/food products to enhance the flavor and nutritional value that subsequently raise the risk of hypersensitivity reactions. Mustard allergy has been reported for many years and is increasing gradually especially in the areas where its consumption is comparatively higher, and it may be considered among the most important food allergies. A number of relevant clinical studies focused on mustard-induced allergic manifestations are summarized in the current review. In addition, the knowledge regarding the immunological as well as biochemical characteristics of mustard allergens that have been known till date and their cross-reactivity with other food allergens have also been discussed here...
November 20, 2017: Clinical Reviews in Allergy & Immunology
https://www.readbyqxmd.com/read/29156294/phospholipase-a1-based-cross-reactivity-among-venoms-of-clinically-relevant-hymenoptera-from-neotropical-and-temperate-regions
#10
Amilcar Perez-Riverol, Luís Gustavo Romani Fernandes, Alexis Musacchio Lasa, José Roberto Aparecido Dos Santos-Pinto, Débora Moitinho Abram, Gabriel Hideki Izuka Moraes, Frederic Jabs, Michaela Miehe, Henning Seismman, Mario Sergio Palma, Ricardo de Lima Zollner, Edzard Spillner, Márcia Regina Brochetto-Braga
Molecular cross-reactivity caused by allergen homology or cross-reactive carbohydrate determinants (CCDs) is a major challenge for diagnosis and immunotherapy of insect venom allergy. Venom phospholipases A1 (PLA1s) are classical, mostly non-glycosylated wasp and ant allergens that provide diagnostic benefit for differentiation of genuine sensitizations from cross-reactivity. As CCD-free molecules, venom PLA1s are not causative for CCD-based cross-reactivity. Little is known however about the protein-based cross-reactivity of PLA1 within vespid species...
November 17, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/29132960/influenza-derived-peptides-cross-react-with-allergens-and-provide-asthma-protection
#11
Chrysanthi Skevaki, Christoph Hudemann, Mikhail Matrosovich, Christian Möbs, Sinu Paul, Andreas Wachtendorf, Bilal Alashkar Alhamwe, Daniel P Potaczek, Stefanie Hagner, Diethard Gemsa, Holger Garn, Alessandro Sette, Harald Renz
BACKGROUND: The hygiene hypothesis is the leading concept to explain the current asthma epidemic, which is built on the observation that a lack of bacterial contact early in life induces allergic Th2 immune responses. OBJECTIVE: Since little is known about the contribution of respiratory viruses in this context, we evaluated the effect of prior influenza-infection on the development of allergic asthma. METHODS: Mice were infected with influenza and once recovered, subjected to an ovalbumin or house dust mite-induced experimental asthma protocol...
November 10, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29130794/allergen-immunotherapy-for-birch-apple-syndrome-what-do-we-know
#12
Cristoforo Incorvaia, Erminia Ridolo, Marina Mauro, Marina Russello, Elide Pastorello
Apple allergy is common in patients allergic to birch pollen, and this is defined as 'birch-apple syndrome'. Allergens responsible for cross-reactivity belong to the pathogenesis-related-10 family, and high homology in the amino acid sequences of the major allergens Bet v 1 from birch and Mal d 1 from apple has been demonstrated. Here we review the literature on the treatment of birch-apple syndrome by allergen immunotherapy. The only allergen immunotherapy method available thus far is based on the administration of birch-pollen extracts, through the subcutaneous or sublingual route, to induce tolerance to Bet v1 and to the homologous allergen Mal d 1...
November 2017: Immunotherapy
https://www.readbyqxmd.com/read/29121407/strong-and-frequent-t-cell-responses-to-the-minor-allergen-phl-p-12-in-spanish-patients-ige-sensitized-to-profilins
#13
Gitte Lund, Stephanie Brand, Tania Ramos, Lucia Jimeno, Patrice Boissy, Francisco Vega, Maria Arina, Lars Harder Christensen, Ilka Hoof, Kåre Hvalsøe Meno, Domingo Barber, Carlos Blanco, Peter Adler Würtzen, Peter Sejer Andersen
BACKGROUND: Profilins are dominant pan-allergens known to cause cross-sensitization, leading to clinical symptoms such as the pollen food syndrome. This study aims to determine the T-cell response to Phl p 12 in profilin-sensitized patients, by measuring the prevalence, strength and cross-reactivity to clinically relevant profilins. METHODS: The release of Phl p allergens from pollen was determined by mass spectrometry and immunochemistry. T-cell responses, epitope mapping and cross-reactivity to profilins (Phl p 12, Ole e 2, Bet v 2, and Mal d 4) were measured in-vitro using PBMCs from 26 Spanish grass allergic donors IgE-sensitized to profilin...
November 9, 2017: Allergy
https://www.readbyqxmd.com/read/29120805/characterization-of-narrow-leaf-lupin-lupinus-angustifolius-l-recombinant-major-allergen-ige-binding-proteins-and-the-natural-%C3%AE-conglutin-counterparts-in-sweet-lupin-seed-species
#14
Jose C Jimenez-Lopez, Rhonda C Foley, Ella Brear, Victoria C Clarke, Elena Lima-Cabello, Jose F Florido, Karam B Singh, Juan D Alché, Penelope M C Smith
β-conglutin has been identified as a major allergen for Lupinus angustifolius seeds. The aim of this study was to evaluate the binding of IgE to five recombinant β-conglutin isoforms (rβ) that we overexpressed and purified and to their natural counterparts in different lupin species and cultivars. Western blotting suggested β-conglutins were the main proteins responsible for the IgE reactivity of the lupin species and cultivars. Newly identified polypeptides from "sweet lupin" may constitute a potential new source of primary or cross-reactive sensitization to lupin, particularly to L...
April 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29120774/a-group-specific-quantitative-real-time-pcr-assay-for-detection-of-crab-a-crustacean-shellfish-allergen-in-complex-food-matrices
#15
Anne C Eischeid, Sarah R Stadig
A real-time PCR assay was developed for detection of crab, a crustacean allergen, in food products. Group-specific primers and probes were developed to detect numerous species of crab. Method validation included tests of detection in complex food matrices, evaluation of commercial food products, and cross-reactivity testing on a wide variety of crustaceans. The method was able to detect several species of crab spiked into complex food matrices at levels ranging from 0.1 to 10(5) parts per million (weight/weight), worked equally well on different platforms, exhibited high specificity for crab over other types of crustaceans, and yielded much higher signals from commercial food products listing crab as an ingredient than from those containing other crustaceans...
April 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29118674/high-frequency-of-ige-sensitization-towards-kiwi-seed-storage-proteins-among-peanut-allergic-individuals-also-reporting-allergy-to-kiwi
#16
Jenny van Odijk, Sigrid Sjölander, Peter Brostedt, Magnus P Borres, Hillevi Englund
Background: IgE sensitization to storage proteins from nuts and seed is often related to severe allergic symptoms. There is a risk of immunological IgE cross-reactivity between storage proteins from different species. The potential clinical implication of such cross-reactivity is that allergens other than the known sensitizer can cause allergic symptoms. Previous studies have suggested that kiwi seed storage proteins may constitute hidden food allergens causing cross-reactive IgE-binding with peanut and other tree nut homologs, thereby mediating a potential risk of causing allergy symptoms among peanut ant tree nut allergic individuals...
2017: Clinical and Molecular Allergy: CMA
https://www.readbyqxmd.com/read/29106961/immunological-analysis-of-food-proteins-using-high-performance-thin-layer-chromatography-immunostaining
#17
Lena Morschheuser, Kathrin Mink, Ramona Horst, Constanze Kallinich, Sascha Rohn
The chromatographic analysis of intact proteins is still challenging, especially when biological functions as antigenicity of proteins or peptides are in the focus. Traditional immunoassays provide information about the entirety of antigenic proteins/peptides, e.g., in ELISA assays. On the other hand, when focusing on the investigation of (cross) reactivity of antibodies, Western blot following gel-electrophoresis represents the method of choice. However, gel-electrophoresis is limited by the molecular weight and therefore, not suitable for peptides ≤3kDa or proteins ≥250kDa...
October 20, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/29096167/crystal-structure-determination-and-analysis-of-11s-coconut-allergen-cocosin
#18
S Vajravijayan, N Nandhagopal, K Gunasekaran
Allergy is an abnormal immune response against an innocuous target. Food allergy is an adverse reaction caused by common foods most well-known being those involving peanuts. Apart from mono sensitized food allergy, cross-reactivity with other food allergens is also commonly observed. To understand the phenomenon of cross-reactivity related to immune response, three dimensional structures of the allergens and their antigenic epitopes has to be analysed in detail. The X-ray crystal structure of Cocosin, a common 11S food allergen from coconut, has been determined at 2...
October 27, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/29072879/cockroach-is-a-major-cross-reactive-allergen-source-in-shrimp-sensitized-rural-children-in-southern-china
#19
Zhaowei Yang, Jiefeng Zhao, Nili Wei, Mulin Feng, Mo Xian, Xu Shi, Zhenyu Zheng, Qiujuan Su, Gary W K Wong, Jing Li
BACKGROUND: Little is known about the prevalence of food allergy (FA) in China. OBJECTIVE: To investigate the prevalence of FA and its disparity between urban and rural areas in southern China. METHODS: Europrevall questionnaire responses were obtained from 5,542 school-age-children in urban Guangzhou and 5,319 in rural Shaoguan. A case-control study enrolled 190 children with adverse reactions (ARs) after food intake as cases, and 212 controls in Guangzhou; 116 cases and 233 controls in Shaoguan...
October 26, 2017: Allergy
https://www.readbyqxmd.com/read/29065418/characterization-of-a-major-allergen-from-mongolian-oak-quercus-mongolica-a-dominant-species-of-oak-in-korea
#20
June Yong Lee, Misuk Yang, Kyoung Yong Jeong, Da Woon Sim, Jin Hee Park, Kyung Hee Park, Jae-Hyun Lee, Jung-Won Park
BACKGROUND: Oaks are the most common trees in Korean forests, and Mongolian oak, Quercus mongolica, is the dominant species. However, no allergen has been characterized from Mongolian oak. In this study, we tried to characterize a major allergen from Mongolian oak. METHODS: A molecule homologous to pathogenesis-related 10 (PR-10)-like protein, Que m 1, was cloned by RT-PCR. Its recombinant protein, along with Que a 1, an allergen from white oak (Q. alba), was produced...
2017: International Archives of Allergy and Immunology
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