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https://www.readbyqxmd.com/read/29437395/visible-light-mediated-nitration-of-protected-anilines
#1
Simon J S Duesel, Burkhard König
The photocatalytic nitration of protected anilines proceeds with riboflavin tetraacetate as organic photoredox catalyst. Sodium nitrite serves as NO2-source in the visible-light driven room temperature reaction. Various nitroanilines are obtained in moderate to good yields without the addition of acid or stoichiometric oxidation agents. The catalytic cycle is closed by aerial oxygen as the terminal oxidant.
February 13, 2018: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/29432804/effects-of-long-term-nitrate-supplementation-on-carbohydrate-metabolism-lipid-profiles-oxidative-stress-and-inflammation-in-male-obese-type-2-diabetic-rats
#2
Sevda Gheibi, Sajad Jeddi, Mattias Carlström, Hanieh Gholami, Asghar Ghasemi
PURPOSE: Supplementation with inorganic nitrate to boost the nitrate-nitrite-nitric oxide (NO) pathway, may act as a potential therapeutic agent in diabetes. The aim of this study was to determine the effects of nitrate on carbohydrate metabolism, lipid profiles, oxidative stress, and inflammation in obese type 2 diabetic rats. METHODS: Male Wistar rats were divided into 4 groups: Control, control + nitrate, diabetes, and diabetes + nitrate. Diabetes was induced using a high-fat diet and low-dose of streptozotocin...
February 10, 2018: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/29432741/dietary-nitrate-protects-submandibular-gland-from-hyposalivation-in-ovariectomized-rats-via-suppressing-cell-apoptosis
#3
Yipu Xu, Baoxing Pang, Liang Hu, Xiaoyu Feng, Lei Hu, Jingsong Wang, Chunmei Zhang, Songlin Wang
Xerostomia, a major oral symptom of menopause, is a subjective feeling of dry mouth associated with oral pain and difficulties in deglutition and speech, which significantly reduces patient's quality of life. Dietary nitrate, which can be converted to nitric oxide, has multiple physiological functions in the body, including antioxidant activity and vasodilatation; however, its protective effect against xerostomia remains poorly understood. The present study aimed to evaluate the effects of dietary nitrate on estrogen deficiency-induced xerostomia...
February 9, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29428840/organ-uptake-and-release-of-inorganic-nitrate-and-nitrite-in-the-pig
#4
Karin E Eriksson, Ting Yang, Mattias Carlström, Eddie Weitzberg
Numerous studies have shown beneficial cardiovascular and metabolic effects of dietary nitrate but the release or uptake of these anions on an organ level is still poorly elucidated. Here we administered sodium nitrate in the pig and measured acute changes in release/uptake of nitrate and nitrite across several organs as well as cardiovascular and metabolic functions. In 17 anesthetized pigs multiple venous catheters and arterial ultrasonic blood flow probes were positioned. After pretreatment with the NO synthase (NOS) inhibitor l-NAME to minimize involvement of NOS-dependent nitrate/nitrite generation, the animals received bolus injections of either sodium nitrate or sodium chloride...
February 8, 2018: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/29412066/alkalinity-and-external-carbon-requirements-for-denitrification-nitrification-of-coke-wastewater
#5
Eleanor Raper, Raymond Fisher, David R Anderson, Tom Stephenson, Ana Soares
The Industrial Emissions Directive (IED) requires that coke wastewater is treated to reach an effluent with <50 mg/L total nitrogen (TN). A shortage of alkalinity (3.6 mg as CaCO3/mg NH4+-N) in the wastewater limited nitrification to 45%. Various compounds were tested as a source of additional alkalinity, with optimal results being found for sodium carbonate, which enabled 95% nitrification at 300 mg/L (as CaCO3). Sodium bicarbonate led to incomplete ammonia oxidation (76%) whilst soda ash prevented nitrite oxidation...
February 7, 2018: Environmental Technology
https://www.readbyqxmd.com/read/29381898/effect-of-natural-pre-converted-nitrite-sources-on-color-development-in-raw-and-cooked-pork-sausage
#6
Ko-Eun Hwang, Tae-Kyung Kim, Hyun-Wook Kim, Dong-Ho Seo, Young-Boong Kim, Ki-Hong Jeon, Yun-Sang Choi
Objective: The effect of pre-converted nitrites from natural sources (spinach, lettuce, celery, and red beet) on color development in raw and cooked pork sausage was investigated in this study. Methods: The pork sausage was manufactured with six treatments: NC (negative control, nitrite free), PC (positive control, 150 ppm sodium nitrite), FS (3.0% fermented spinach extracts), FL (3.0% fermented lettuce extracts), FC (3.0% fermented celery extracts), and FR (3.0% fermented red beet extracts)...
January 26, 2018: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/29358811/a-rapid-and-sensitive-evaluation-of-nitrite-content-in-saudi-arabian-processed-meat-and-poultry-using-a-novel-ultra-performance-liquid-chromatography-mass-spectrometry-method
#7
Masoom Raza Siddiqui, Saikh Mohammad Wabaidur, Moonis Ali Khan, Zeid A ALOthman, M Z A Rafiquee, Ayoub Abdullah Alqadami
Quantitative assessment of nitrite (NO2-) anion was performed using a newly developed high throughput ultra performance liquid chromatography-mass spectrometric (UPLC-MS) method. The nitrite determination with the proposed method using micellar mobile phase was unknown. Selected ion reaction mode using negative electrospray ionization was adopted for the identification and quantitative analysis of nitrite. The chromatographic separation was performed using BEH C-18 column and a micellar mobile phase consisted of sodium dodecyl sulphate and acetonitrile in ratio 30:70 was used...
January 2018: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/29355552/maternal-hypertension-and-feto-placental-growth-restriction-is-reversed-by-sildenafil-evidence-of-independent-effects-of-circulating-nitric-oxide-levels
#8
Victor Hugo Gonçalves-Rizzi, José Sérgio Possomato-Vieira, Regina Aparecida Nascimento, Mayara Caldeira-Dias, Carlos Alan Dias-Junior
Sildenafil has shown nitric oxide (NO)-independent pleiotropic effects, however the mechanisms involved are unclear. We investigated the protective effects of sildenafil against hypertension in pregnancy and feto-placental growth restriction induced by NO inhibition, and if sodium nitrite-derived NO formation influences sildenafil effects. We evaluated the plasmatic levels of NO metabolites, cyclic guanosine monophosphate (cGMP), oxidative stress and myeloperoxidase, which are involved in endothelial dysfunction during hypertension in pregnancy...
January 17, 2018: European Journal of Pharmacology
https://www.readbyqxmd.com/read/29339610/effect-of-precursor-type-on-the-reduction-of-concentrated-nitrate-using-zero-valent-copper-and-sodium-borohydride
#9
Tihitinna Asmellash Belay, C Y Lin, H M Hsiao, M F Chang, J C Liu
In this study, we demonstrated that the choice of precursor has a strong effect on the reduction of nitrate (NO3-) using zero-valent copper (Cu0) synthesized by sodium borohydride (NaBH4). Different precursors: CuSO4, CuO, Cu2O, Cu powder, and Cu mesh were used to reduce NO3- at 677 mg-N/L under the reducing conditions of NaBH4. Compared with the prehydrolyzed samples, those prepared without prehydrolysis exhibited lower reduction rates, longer times and higher concentrations of nitrite (NO2-) intermediate...
January 2018: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/29323037/bone-marrow-derived-mesenchymal-stem-cells-ameliorate-sodium-nitrite-induced-hypoxic-brain-injury-in-a-rat-model
#10
Elham H A Ali, Omar A Ahmed-Farid, Amany A E Osman
Sodium nitrite (NaNO2) is an inorganic salt used broadly in chemical industry. NaNO2 is highly reactive with hemoglobin causing hypoxia. Mesenchymal stem cells (MSCs) are capable of differentiating into a variety of tissue specific cells and MSC therapy is a potential method for improving brain functions. This work aims to investigate the possible therapeutic role of bone marrow-derived MSCs against NaNO2 induced hypoxic brain injury. Rats were divided into control group (treated for 3 or 6 weeks), hypoxic (HP) group (subcutaneous injection of 35 mg/kg NaNO2 for 3 weeks to induce hypoxic brain injury), HP recovery groups N-2wR and N-3wR (treated with the same dose of NaNO2 for 2 and 3 weeks respectively, followed by 4-week or 3-week self-recovery respectively), and MSCs treated groups N-2wSC and N-3wSC (treated with the same dose of NaNO2 for 2 and 3 weeks respectively, followed by one injection of 2 × 106 MSCs via the tail vein in combination with 4 week self-recovery or intravenous injection of NaNO2 for 1 week in combination with 3 week self-recovery)...
December 2017: Neural Regeneration Research
https://www.readbyqxmd.com/read/29302847/a-green-non-acid-catalyzed-process-for-direct-n-n-c-group-formation-comprehensive-study-modeling-and-optimization
#11
Vahid Khakyzadeh, Mohammad Ali Zolfigol, Fatemeh Derakhshan-Panah, Majid Jafarian, Mir Vahid Miri, Maryam Gilandoust
The aim of this work is to introduce, model, and optimize a new non-acid-catalyzed system for a direct N[Formula: see text]N-C bond formation. By reacting naphthols or phenol with anilines in the presence of the sodium nitrite as nitrosonium ([Formula: see text] source and triethylammonium acetate (TEAA), a N[Formula: see text]N-C group can be formed in non-acid media. Modeling and optimization of the reaction conditions were investigated by response surface method. Sodium nitrite, TEAA, and water were chosen as variables, and reaction yield was also monitored...
January 4, 2018: Molecular Diversity
https://www.readbyqxmd.com/read/29279966/betulinic-acid-alleviates-dextran-sulfate-sodium-induced-colitis-and-visceral-pain-in-mice
#12
Jaspreet Kalra, Madhu Cholenahalli Lingaraju, Karikalan Mathesh, Dhirendra Kumar, Subhashree Parida, Thakur Uttam Singh, Anil Kumar Sharma, Dinesh Kumar, Surendra Kumar Tandan
Betulinic acid (BA) exhibits many biological effects including anti-inflammatory and anti-oxidant activities. Free radicals and pro-inflammatory mediators play an important role in the pathology of inflammatory bowel disease (IBD) and associated pain. We, therefore, examined the anti-oxidant, anti-inflammatory, and anti-nociceptive potential of BA in colitis. Colitis was induced with 3% (w/v) dextran sulfate sodium (DSS) in drinking water in mice for 1to7 days. BA (3, 10 and 30 mg/kg) was given orally for 0 to 7 days...
December 26, 2017: Naunyn-Schmiedeberg's Archives of Pharmacology
https://www.readbyqxmd.com/read/29240267/acute-oral-dose-of-sodium-nitrite-causes-redox-imbalance-and-dna-damage-in-rat-kidney
#13
Fariheen Aisha Ansari, Shaikh Nisar Ali, Aijaz Ahmed Khan, Riaz Mahmood
Sodium nitrite (NaNO2) is widely used as a food additive and preservative in fish and meat products. We have evaluated the effect of a single acute oral dose of NaNO2 on oxidative stress parameters, antioxidant capacity and DNA in rat kidney. Male Wistar rats were divided into four groups and given single oral dose of NaNO2 at 20, 40, 60 and 75 mg/kg body weight; untreated rats served as the control group. All animals in NaNO2 -treated groups showed marked alterations in various parameters of oxidative stress as compared to the control group...
December 14, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29236024/heterotrophic-nitrogen-removal-in-bacillus-sp-k5-involvement-of-a-novel-hydroxylamine-oxidase
#14
Yunlong Yang, Ershu Lin, Shaobin Huang
An aerobic denitrifying bacterium isolated from a bio-trickling filter treating NOx, Bacillus sp. K5, is able to convert ammonium to nitrite, in which hydroxylamine oxidase (HAO) plays a critical role. In the present study, the performance for simultaneous nitrification and denitrification was investigated with batch experiments and an HAO was purified by an anion-exchange and gel-filtration chromatography from strain K5. The purified HAO's molecular mass was determined by SDS-PAGE and its activity by measuring the change in the concentration of ferricyanide, the electron acceptor...
December 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/29226063/advances-in-antimicrobial-photodynamic-inactivation-at-the-nanoscale
#15
Nasim Kashef, Ying-Ying Huang, Michael R Hamblin
The alarming worldwide increase in antibiotic resistance amongst microbial pathogens necessitates a search for new antimicrobial techniques, which will not be affected by, or indeed cause resistance themselves. Light-mediated photoinactivation is one such technique that takes advantage of the whole spectrum of light to destroy a broad spectrum of pathogens. Many of these photoinactivation techniques rely on the participation of a diverse range of nanoparticles and nanostructures that have dimensions very similar to the wavelength of light...
August 2017: Nanophotonics
https://www.readbyqxmd.com/read/29223781/enhanced-sludge-solubilization-and-dewaterability-by-synergistic-effects-of-nitrite-and-freezing
#16
Faqian Sun, KeKe Xiao, Wenyu Zhu, Nipuna Withanage, Yan Zhou
In this study, nitrite was added into sludge during freezing process to evaluate its role in waste activated sludge (WAS) solubilization and effect on sludge dewatering characteristics. The results showed that the introduction of 100 mg L-1 of nitrite could increase dissolved organic carbon (DOC) concentration from 29.5 to 48.8 mg DOC g-1 VSS under freezing conditions. More DOC was released with the increase of nitrite concentration. Freezing temperature, or freezing speed, also played a role in sludge solubilization...
November 30, 2017: Water Research
https://www.readbyqxmd.com/read/29219187/integrated-molecular-biochemical-and-physiological-assessment-unravels-key-extraction-method-mediated-influences-on-rat-neonatal-cardiomyocytes
#17
Leonardo Jensen, Elida Neri, Vinicius Bassaneze, Nathalia Corrêa De Almeida Oliveira, Rafael Dariolli, Thiago Lauro Turaça, Débora Levy, Douglas Veronez, Mariana Sacrini Ayres Ferraz, Adriano Mesquita Alencar, Sérgio Paulo Bydlowski, Idágene Aparecida Cestari, José Eduardo Krieger
Neonatal cardiomyocytes are instrumental for disease modeling, but the effects of different cell extraction methods on basic cell biological processes remain poorly understood. We assessed the influence of two popular methods to extract rat neonatal cardiomyocytes, Pre-plating (PP) and Percoll (PC) on cell structure, metabolism, and function. Cardiomyocytes obtained from PP showed higher gene expression for troponins, titin, and potassium and sodium channels compared to PC. Also, PP cells displayed higher levels of troponin I protein...
December 8, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29212831/impaired-pulmonary-arterial-vasoconstriction-and-nitric-oxide-mediated-relaxation-underlie-severe-pulmonary-hypertension-in-sugen-hypoxia-rat-model
#18
Helen Christou, Hannes Hudalla, Zoe Michael, Evgenia Filatava, Jun Li, Minglin Zhu, Jose Possomato-Vieira, Carlos Dias-Junior, Stella Kourembanas, Raouf A Khalil
Pulmonary vasoreactivity could determine the responsiveness to vasodilators and in turn the prognosis of pulmonary hypertension (PH). We hypothesized that pulmonary vasoreactivity is impaired, and examined the underlying mechanisms, in the sugen-hypoxia rat model of severe PH. Male Sprague-Dawley rats were injected with sugen (20 mg/kg sc) and exposed to hypoxia (9% O2) for 3 weeks followed by 4 weeks in normoxia (Su/Hx), or treated with sugen alone (Su) or hypoxia alone (Hx) or neither (Nx). After hemodynamic measurements, the heart was assessed for right ventricular hypertrophy (Fulton's Index), the pulmonary artery, aorta and mesenteric arteries were isolated for vascular function studies, and contractile markers were measured in pulmonary artery using quantitative PCR...
December 6, 2017: Journal of Pharmacology and Experimental Therapeutics
https://www.readbyqxmd.com/read/29201569/denitrifying-metabolism-of-the-methylotrophic-marine-bacterium-methylophaga-nitratireducenticrescens-strain-jam1
#19
Florian Mauffrey, Alexandra Cucaita, Philippe Constant, Richard Villemur
Background: Methylophaga nitratireducenticrescens strain JAM1 is a methylotrophic, marine bacterium that was isolated from a denitrification reactor treating a closed-circuit seawater aquarium. It can sustain growth under anoxic conditions by reducing nitrate ([Formula: see text]) to nitrite ([Formula: see text]). These physiological traits are attributed to gene clusters that encode two dissimilatory nitrate reductases (Nar). Strain JAM1 also contains gene clusters encoding two nitric oxide (NO) reductases and one nitrous oxide (N2O) reductase, suggesting that NO and N2O can be reduced by strain JAM1...
2017: PeerJ
https://www.readbyqxmd.com/read/29175357/hemodynamic-effects-of-iv-sodium-nitrite-in-hospitalized-comatose-survivors-of-out-of-hospital-cardiac-arrest
#20
Cameron Dezfulian, Michele Olsufka, Deborah Fly, Sue Scruggs, Rose Do, Charles Maynard, Graham Nichol, Francis Kim
BACKGROUND: Patients resuscitated from cardiac arrest have brain and cardiac injury. Recent animal studies suggest that the administration of sodium nitrite after resuscitation from 12min of asystole limits acute cardiac dysfunction and improves survival and neurologic outcomes. It has been hypothesized that low doses of IV sodium nitrite given during resuscitation of out of hospital cardiac arrest (OHCA) will improve survival. Low doses of sodium nitrite (e.g., 9.6mg of sodium nitrite) are safe in healthy individuals, however the effect of nitrite on blood pressure in resuscitated cardiac arrest patients is unknown...
January 2018: Resuscitation
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