keyword
MENU ▼
Read by QxMD icon Read
search

Pentapeptide

keyword
https://www.readbyqxmd.com/read/27909427/biocombinatorial-synthesis-of-novel-lipopeptides-by-com-domain-mediated-reprogramming-of-the-plipastatin-nrps-complex
#1
Hongxia Liu, Ling Gao, Jinzhi Han, Zhi Ma, Zhaoxin Lu, Chen Dai, Chong Zhang, Xiaomei Bie
Both donors and acceptors of communication-mediating (COM) domains are essential for coordinating intermolecular communication within nonribosomal peptides synthetases (NRPSs) complexes. Different sets of COM domains provide selectivity, allowing NRPSs to utilize different natural biosynthetic templates. In this study, novel lipopeptides were synthesized by reprogramming the plipastatin biosynthetic machinery. A Thr-to-Asp point mutation was sufficient to shift the selectivity of the donor COM domain of ppsB toward that of ppsD...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27890013/in-silico-characterization-of-a-hypothetical-protein-rv1288-of-mycobacterium-tuberculosis-containing-an-esterase-signature-and-an-uncommon-lyte-domain
#2
Arbind Kumar, Pratibha Maan, Gurpreet Singh, Jagdeep Kaur
BACKGROUND: Death toll due to tuberculosis is still rising day by day. Whole genome sequence of Mycobacterium tuberculosis has provided a platform to conduct research in order to identify the probable drug target. OBJECTIVES: Out of 4000 gene products of M. tuberculosis, approximately 40% of proteins are annotated as hypothetical. Identifying and characterizing these proteins could provide a new prescriptive for developing new TB drugs. Rv1288, a protein of M. tuberculosis H37Rv has been annotated as a hypothetical protein in database...
November 24, 2016: Current Computer-aided Drug Design
https://www.readbyqxmd.com/read/27887965/muropeptide-signature-of-inhibitors-of-protein-synthesis-correlates-with-%C3%AE-lactam-synergism-against-methicillin-resistant-staphylococcus-aureus
#3
Bertrand Guignard, Paul A Majcherczyk, Cédric Monachon, Jacques Vouillamoz, Philippe Moreillon
Quinupristin/dalfopristin (Q/D) and β-lactams interact positively against methicillin-resistant Staphylococcus aureus (MRSA). The effect extends to other inhibitors of protein synthesis, but not to inhibitors of polynucleotide synthesis or assembly, or to Q/D plus non-β-lactam cell wall inhibitors. Moreover, electron microscopy studies have correlated this effect with a thickened cell wall. In this study, we sought to determine whether inhibitors of protein synthesis might produce a specific peptidoglycan muropeptide signature that would correlate with their positive β-lactam interaction...
November 16, 2016: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/27879684/role-of-osteogenic-growth-peptide-ogp-and-ogp-10-14-in-bone-regeneration-a-review
#4
REVIEW
Suzane C Pigossi, Marcell C Medeiros, Sybele Saska, Joni A Cirelli, Raquel M Scarel-Caminaga
Bone regeneration is a process that involves several molecular mediators, such as growth factors, which directly affect the proliferation, migration and differentiation of bone-related cells. The osteogenic growth peptide (OGP) and its C-terminal pentapeptide OGP(10-14) have been shown to stimulate the proliferation, differentiation, alkaline phosphatase activity and matrix mineralization of osteoblastic lineage cells. However, the exact molecular mechanisms that promote osteoblastic proliferation and differentiation are not completely understood...
November 22, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27878897/minimal-immune-determinants-connect-zika-virus-human-cytomegalovirus-and-toxoplasma-gondii-to-microcephaly-related-human-proteins
#5
Guglielmo Lucchese, Darja Kanduc
Aiming at the molecular definition of the relationship(s) between prenatal infections and fetal microcephaly and using pentapeptides as minimal immune determinants, we analyzed the peptide matching between proteins from infectious agents involved in microcephalic syndromes (namely Zika virus, human Cytomegalovirus, and Toxoplasma gondii) and human proteins that, when altered, have been specifically associated with microcephaly. We report that an unexpected high number of epitopic pentapeptides (ie, 34) are common to the three fetopathogenic agents and repeatedly occur throughout an important number of microcephaly-related human proteins...
November 23, 2016: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/27864999/a-synthetic-toolbox-for-the-in-situ-formation-of-functionalized-homo-and-heteropolysaccharide-based-hydrogel-libraries
#6
Nick Dibbert, Andreas Krause, Julio-Cesar Rios-Camacho, Ina Gruh, Andreas Kirschning, Gerald Dräger
A synthetic toolbox for the introduction of aldehydo and hydrazido groups into the polysaccharides hyaluronic acid, alginate, dextran, pullulan, glycogen, and carboxymethyl cellulose and their use for hydrogel formation is reported. Upon mixing differently functionalized polysaccharides derived from the same natural precursor, hydrazone cross-linking takes place, which results in formation of a hydrogel composed of one type of polysaccharide backbone. Likewise, hydrogels based on two different polysaccharide strands can be formed after mixing the corresponding aldehydo- and hydrazido-modified polysaccharides...
November 16, 2016: Chemistry: a European Journal
https://www.readbyqxmd.com/read/27851780/characterisation-of-a-new-family-of-carboxyl-esterases-with-an-osmc-domain
#7
Mai-Britt V Jensen, Louise E Horsfall, Caroline Wardrope, Peter D Togneri, Jon Marles-Wright, Susan J Rosser
Proteins in the serine esterase family are widely distributed in bacterial phyla and display activity against a range of biologically produced and chemically synthesized esters. A serine esterase from the psychrophilic bacterium Pseudoalteromonas arctica with a C-terminal OsmC-like domain was recently characterized; here we report on the identification and characterization of further putative esterases with OsmC-like domains constituting a new esterase family that is found in a variety of bacterial species from different environmental niches...
2016: PloS One
https://www.readbyqxmd.com/read/27806933/mechanisms-of-fluoroquinolone-resistance-in-mycobacterium-tuberculosis
#8
Zhang Yujiao, Li Xiaojing, Mi Kaixia
Tuberculosis, caused by the pathogen Mycobacterium tuberculosis, is one of the world's deadliest bacterial infectious disease. It is still a global-health threat, particularly because of the drug-resistant forms. Fluoroquinolones, with target of gyrase, are among the drugs used to treat tuberculosis. However, their widespread use has led to bacterial resistance. The molecular mechanisms of fluoroquinolone resistance in mycobacterium tuberculosis have been reported, such as DNA gyrase mutations, drug efflux pumps system, bacterial cell wall thickness and pentapeptide proteins (MfpA) mediated regulation of gyrase...
October 20, 2016: Yi Chuan, Hereditas
https://www.readbyqxmd.com/read/27778504/characterization-of-the-reversed-phase-chromatographic-behavior-of-pegylated-peptides-based-on-the-poly-ethylene-glycol-dispersity
#9
Eun Ji Park, Dong Hee Na
The separation mechanism of PEGylated peptides in reversed-phase high-performance liquid chromatography (RP-HPLC) is complex, because the PEGylated molecules exhibit physicochemical properties that are different from those of the parent molecules and have heterogeneous structure. Since most separation studies have focused on the hydrophobicity of the peptide relative to poly(ethylene glycol) (PEG), the role of PEG in the separation of PEGylated peptides on RP-HPLC is not clear. To elucidate the effect of the attached PEG on the retention of PEGylated peptides on RP-HPLC, the mono-PEGylated forms of collagen pentapeptide and octreotide were fractionated drop-by-drop from the outlet of the HPLC system and each drop was subjected to matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)...
October 25, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27773717/cardioprotective-effects-of-monocyte-locomotion-inhibitory-factor-on-myocardial-ischemic-injury-by-targeting-vimentin
#10
Shu Jiang, Yulan Liu, Jing Wang, Yue Zhang, Yaocheng Rui, Yuefan Zhang, Tiejun Li
Monocyte locomotion inhibitory factor (MLIF), a heat-stable pentapeptide produced by Entamoeba histolytica, has anti-inflammatory function and protective effect on ischemic stroke. In this study, we evaluated the effect of MLIF on myocardial ischemia. The myocardial ischemia reperfusion (I/R) model was established by ligation of the left anterior descending branch (LAD) using 8-0 silk suture for 30min followed by 3h reperfusion in mice. After sacrifice, the serum concentrations of cardiac troponin I (cTnI), creatine kinase (CK), lactate dehydrogenase (LDH) as well as the heart infarct size were measured...
October 20, 2016: Life Sciences
https://www.readbyqxmd.com/read/27746119/development-of-the-first-internally-quenched-fluorescent-substrates-of-human-cathepsin-c-the-application-in-the-enzyme-detection-in-biological-samples
#11
Monika Łęgowska, Yveline Hamon, Anna Wojtysiak, Renata Grzywa, Marcin Sieńczyk, Timo Burster, Brice Korkmaz, Adam Lesner
Cathepsin C is a widely expressed cysteine exopeptidase that is mostly recognized for the activation of the granule-associated proinflammatory serine proteases in neutrophils, cytotoxic T lymphocytes and mast cells. It has been shown that the enzyme can be secreted extracellularly; however, its occurrence in human bodily fluids/physiological samples has not been thoroughly studied. In the course of this study, the first fluorescence resonance energy transfer peptides for the measurement of the activity of human cathepsin C were designed and synthesized...
October 13, 2016: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/27734910/development-of-a-one-pot-assay-for-screening-and-identification-of-mur-pathway-inhibitors-in-mycobacterium-tuberculosis
#12
Kandasamy Eniyan, Anuradha Kumar, Geetha Vani Rayasam, Andrej Perdih, Urmi Bajpai
The cell wall of Mycobacterium tuberculosis (Mtb) consists of peptidoglycan, arabinogalactan and mycolic acids. The cytoplasmic steps in the peptidoglycan biosynthetic pathway, catalyzed by the Mur (A-F) enzymes, involve the synthesis of UDP-n-acetylmuramyl pentapeptide, a key precursor molecule required for the formation of the peptidoglycan monomeric building blocks. Mur enzymes are indispensable for cell integrity and their lack of counterparts in eukaryotes suggests them to be promising Mtb drug targets...
October 13, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27716106/in-vivo-functional-and-molecular-characterization-of-the-penicillin-binding-protein-4-dacb-of-pseudomonas-aeruginosa
#13
Cristian Gustavo Aguilera Rossi, Paulino Gómez-Puertas, Juan Alfonso Ayala Serrano
BACKGROUND: Community and nosocomial infections by Pseudomonas aeruginosa still create a major therapeutic challenge. The resistance of this opportunist pathogen to β-lactam antibiotics is determined mainly by production of the inactivating enzyme AmpC, a class C cephalosporinase with a regulation system more complex than those found in members of the Enterobacteriaceae family. This regulatory system also participates directly in peptidoglycan turnover and recycling. One of the regulatory mechanisms for AmpC expression, recently identified in clinical isolates, is the inactivation of LMM-PBP4 (Low-Molecular-Mass Penicillin-Binding Protein 4), a protein whose catalytic activity on natural substrates has remained uncharacterized until now...
October 6, 2016: BMC Microbiology
https://www.readbyqxmd.com/read/27713572/neuroprotective-pentapeptide-cn-105-improves-functional-and-histological-outcomes-in-a-murine-model-of-intracerebral-hemorrhage
#14
Beilei Lei, Michael L James, Ji Liu, Guanen Zhou, Talaignair N Venkatraman, Christopher D Lascola, Shawn K Acheson, Laura G Dubois, Daniel T Laskowitz, Haichen Wang
Presently, no pharmacological treatments have been demonstrated to improve long-term functional outcomes following intracerebral hemorrhage (ICH). Clinical evidence associates apolipoprotein E (apoE) genotype with ICH incidence and outcome. While apoE modifies neuroinflammatory responses through its adaptive role in glial downregulation, intact apoE holoprotein is too large to cross the blood-brain barrier (BBB). Therefore, we developed a 5-amino acid peptide - CN-105 - that mimics the polar face of the apoE helical domain involved in receptor interactions...
October 7, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27711177/structural-basis-of-the-heterodimer-formation-between-cell-shape-determining-proteins-csd1-and-csd2-from-helicobacter-pylori
#15
Doo Ri An, Ha Na Im, Jun Young Jang, Hyoun Sook Kim, Jieun Kim, Hye Jin Yoon, Dusan Hesek, Mijoon Lee, Shahriar Mobashery, Soon-Jong Kim, Se Won Suh
Colonization of the human gastric mucosa by Helicobacter pylori requires its high motility, which depends on the helical cell shape. In H. pylori, several genes (csd1, csd2, csd3/hdpA, ccmA, csd4, csd5, and csd6) play key roles in determining the cell shape by alteration of cross-linking or by trimming of peptidoglycan stem peptides. H. pylori Csd1, Csd2, and Csd3/HdpA are M23B metallopeptidase family members and may act as d,d-endopeptidases to cleave the d-Ala4-mDAP3 peptide bond of cross-linked dimer muropeptides...
2016: PloS One
https://www.readbyqxmd.com/read/27680590/structure-activity-relationship-of-cyclic-pentapeptide-malformins-as-fibrinolysis-enhancers
#16
Yukio Koizumi, Kenichiro Nagai, Keiji Hasumi, Keiji Kuba, Toshihiro Sugiyama
The formation of blood clots in blood vessels causes severe ischemic diseases such as cerebral infarction and myocardial infarction. While searching for microbial products that increase fibrinolytic activity using an in vitro fibrin degradation assay, we found malformin A1, a disulfide form of cyclo(-d-Cys-d-Cys-l-Val-d-Leu-l-Ile-), as an active compound. In this study, we synthesized malformin derivatives using a solid-phase peptide synthesis method and evaluated their fibrinolytic activity and cytotoxicity...
September 16, 2016: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/27669322/the-peptidoglycan-pattern-of-staphylococcus-carnosus-tm300-detailed-analysis-and-variations-due-to-genetic-and-metabolic-influences
#17
Julia Deibert, Daniel Kühner, Mark Stahl, Elif Koeksoy, Ute Bertsche
The Gram-positive bacterium Staphylococcus carnosus (S. carnosus) TM300 is an apathogenic staphylococcal species commonly used in meat starter cultures. As with all Gram-positive bacteria, its cytoplasmic membrane is surrounded by a thick peptidoglycan (PGN) or murein sacculus consisting of several layers of glycan strands cross-linked by peptides. In contrast to pathogenic staphylococci, mainly Staphylococcus aureus (S. aureus), the chemical composition of S. carnosus PGN is not well studied so far. UPLC/MS analysis of enzymatically digested S...
2016: Antibiotics
https://www.readbyqxmd.com/read/27667506/electrophilic-rna-for-peptidyl-rna-synthesis-and-site-specific-cross-linking-with-trna-binding-enzymes
#18
Matthieu Fonvielle, Nicolas Sakkas, Laura Iannazzo, Chloé Le Fournis, Delphine Patin, Dominique Mengin-Lecreulx, Afaf El-Sagheer, Emmanuelle Braud, Sébastien Cardon, Tom Brown, Michel Arthur, Mélanie Etheve-Quelquejeu
RNA functionalization is challenging due to the instability of RNA and the limited range of available enzymatic reactions. We developed a strategy based on solid phase synthesis and post-functionalization to introduce an electrophilic site at the 3' end of tRNA analogues. The squarate diester used as an electrophile enabled sequential amidation and provided asymmetric squaramides with high selectivity. The squaramate-RNAs specifically reacted with the lysine of UDP-MurNAc-pentapeptide, a peptidoglycan precursor used by the aminoacyl-transferase FemXWv for synthesis of the bacterial cell wall...
October 17, 2016: Angewandte Chemie
https://www.readbyqxmd.com/read/27662793/investigating-the-addition-of-collagen-and-its-integrin-binding-sequence-rgd-to-glass-polyalkenoate-in-terms-of-material-and-cellular-properties-to-explore-a-more-biocompatible-method-of-root-caries-restoration
#19
A M Salem, S J Jones, I R Ellis, R G Chadwick
AIMS: Placement of a restoration to treat root caries disrupts many tissues. There is scope for the restorative material to interact with these to augment reductions in micro leakage afforded by an adhesive restorative material. OBJECTIVES: 1) To investigate the effects of incorporating bioactive molecules into a glass polyalkenoate (GPA) 2) To quantify the changes in physical properties of the material. METHODS: Biocompatibility of the GPA cement (Chemfil Superior, Dentsply De Trey, Konstanz, Germany) in unmodified and modified forms was ascertained using cell culture techniques...
November 2016: Journal of Dentistry
https://www.readbyqxmd.com/read/27660028/angiotensin-1-5-an-active-mediator-of-renin-angiotensin-system-stimulates-anp-secretion-via-mas-receptor
#20
Lamei Yu, Kuichang Yuan, Hoang Thi Ai Phuong, Byung Mun Park, Suhn Hee Kim
Angiotensin-(1-5) [Ang-(1-5)], which is a metabolite of Angiotensin-(1-7) [Ang-(1-7)] catalyzed by angiotensin-converting enzyme (ACE), is a pentapeptide of the renin-angiotensin system (RAS). It has been reported that Ang-(1-7) and Ang-(1-9) stimulate the secretion of atrial natriuretic peptide (ANP) via Mas receptor (Mas R) and Ang II type 2 receptor (AT2R), respectively. However, it still remains unknown whether Ang-(1-5) has a similar function to Ang-(1-7). We investigated the effect of Ang-(1-5) on ANP secretion and to define its signaling pathway using isolated perfused beating rat atria...
December 2016: Peptides
keyword
keyword
111832
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"