keyword
MENU ▼
Read by QxMD icon Read
search

KLF4

keyword
https://www.readbyqxmd.com/read/28086833/delta-like-4-notch-signaling-promotes-apc-min-tumor-initiation-through-angiogenic-and-non-angiogenic-related-mechanisms
#1
Marina Badenes, Alexandre Trindade, Hugo Pissarra, Luís Lopes-da-Costa, António Duarte
BACKGROUND: Delta like 4 (Dll4)/Notch signaling is a key regulator of tumor angiogenesis. Additionally, the role of Dll4 has been studied on tumor stem cells. However, as these cells are implicated in tumor angiogenesis, it is conceivable that the effect of Dll4 on these cells may be a consequence of its angiogenic function. Our aim was to evaluate the expression and dissect the functions of Dll4 in the Apc (Min/+) model of colorectal cancer. METHODS: We evaluated the protein expression pattern of Dll4 and other Notch members in the Apc (Min/+) tumors relatively to the normal gut and compared endothelial-specific with ubiquitous Dll4 knockout mice on an Apc (Min/+) background...
January 13, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28079980/induction-of-mesenchymal-stem-cell-differentiation-in-the-absence-of-soluble-inducer-for-cutaneous-wound-regeneration-by-a-chitin-nanofibers-based-hydrogel
#2
Kangquan Shou, Yao Huang, Baiwen Qi, Xiang Hu, Zhanjun Ma, Ang Lu, Chao Jian, Lina Zhang, Aixi Yu
Transplantation of bone marrow mesenchymal stem cells (BMSCs) has been considered as a promising strategy for wound healing. However, poor viability of engrafted BMSCs and limited capabilities of differentiation into the desired cell types in wounds often hinder their application. Few studies report the induction of BMSCs differentiation into the skin regeneration related cell types using natural biopolymer, e.g., chitin and its derivative. Here we utilized a chitin nanofibers(CNFs) hydrogel as a directive cue to induce BMSCs differentiation for enhancing cutaneous wound regeneration in the absence of cell-differentiating factors...
January 12, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28079892/hif-2%C3%AE-and-oct4-have-synergistic-effects-on-survival-and-myocardial-repair-of-very-small-embryonic-like-mesenchymal-stem-cells-in-infarcted-hearts
#3
Shaoheng Zhang, Lan Zhao, Jiahong Wang, Nannan Chen, Jian Yan, Xin Pan
Poor cell survival and limited functional benefits have restricted mesenchymal stem cell (MSC) efficacy for treating myocardial infarction (MI), suggesting that a better understanding of stem cell biology is needed. The transcription factor HIF-2α is an essential regulator of the transcriptional response to hypoxia, which can interact with embryonic stem cells (ESCs) transcription factor Oct4 and modulate its signaling. Here, we obtained very small embryonic-like mesenchymal stem cells (vselMSCs) from MI patients, which possessed the very small embryonic-like stem cells' (VSELs) morphology as well as ESCs' pluripotency...
January 12, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28078190/loss-of-quiescence-and-self-renewal-capacity-of-hematopoietic-stem-cell-in-an-in-vitro-leukemic-niche
#4
Natalia-Del Pilar Vanegas, Jean-Paul Vernot
BACKGROUND: Leukemic and mesenchymal stem cells interact in the leukemic microenvironment and affect each other differently. This interplay has also important implications for the hematopoietic stem cell (HSC) biology and function. This study evaluated human HSC self-renewal potential and quiescence in an in vitro leukemic niche without leukemic cells. METHODS: A leukemic niche was established by co-culturing mesenchymal stem cells with a fresh conditioned medium obtained from a leukemic (REH) cell line...
2017: Experimental Hematology & Oncology
https://www.readbyqxmd.com/read/28068326/klf4-is-regulated-by-ras-raf-mek-erk-signaling-through-e2f1-and-promotes-melanoma-cell-growth
#5
M Riverso, V Montagnani, B Stecca
Melanoma is the most lethal form of skin cancer and treatment of metastatic melanoma remains challenging. BRAF/MEK inhibitors show only temporary benefit due the occurrence of resistance and immunotherapy is effective only in a subset of patients. To improve patient survival, there is a need to better understand molecular mechanisms that drive melanoma growth and operate downstream of the mitogen activated protein kinase (MAPK) signaling. The Krüppel-like factor 4 (KLF4) is a zinc-finger transcription factor that plays a critical role in embryonic development, stemness and cancer, where it can act either as oncogene or tumor suppressor...
January 9, 2017: Oncogene
https://www.readbyqxmd.com/read/28068319/fbxo32-suppresses-breast-cancer-tumorigenesis-through-targeting-klf4-to-proteasomal-degradation
#6
H Zhou, Y Liu, R Zhu, F Ding, Y Wan, Y Li, Z Liu
Krüppel-like factor 4 (KLF4, GKLF) is a zinc-finger transcription factor involved in a large variety of cellular processes, including apoptosis, cell cycle progression, as well as stem cell renewal. KLF4 is critical for cell fate decision and has an ambivalent role in tumorigenesis. Emerging data keep reminding us that KLF4 dysregulation either facilitates or impedes tumor progression, making it important to clarify the regulating network of KLF4. Like most transcription factors, KLF4 has a rather short half-life within the cell and its turnover must be carefully orchestrated by ubiquitination and ubiquitin-proteasome system...
January 9, 2017: Oncogene
https://www.readbyqxmd.com/read/28066612/elevated-hotair-expression-associated-with-cisplatin-resistance-in-non-small-cell-lung-cancer-patients
#7
Ming-Yue Liu, Xi-Qing Li, Tian-Hui Gao, Yao Cui, Ning Ma, Yun Zhou, Guo-Jun Zhang
BACKGROUND: This study investigated the mechanism of drug resistance in non-small cell lung cancer (NSCLC) patients. We specifically studied whether long noncoding RNAs influence drug resistance in NSCLC to discover new therapeutic targets to increase the survival rate of drug-resistant NSCLC patients. METHODS: Tissue samples were collected from NSCLC patients, and total RNA was isolated for assessment of HOTAIR expression and drug resistance status. MTT assays, tumor sphere formation assays, and western blot were performed to cytologically determine the relationship between HOTAIR expression and cisplatin resistance, as well as to elucidate the potential molecular mechanism involved...
November 2016: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/28066139/regulation-of-vascular-smooth-muscle-phenotype-by-cross-regulation-of-kr%C3%A3-ppel-like-factors
#8
Jung Min Ha, Sung Ji Yun, Seo Yeon Jin, Hye Sun Lee, Sun Ja Kim, Hwa Kyoung Shin, Sun Sik Bae
Regulation of vascular smooth muscle cell (VSMC) phenotype plays an essential role in many cardiovascular diseases. In the present study, we provide evidence that krüppel-like factor 8 (KLF8) is essential for tumor necrosis factor α (TNFα)-induced phenotypic conversion of VSMC obtained from thoracic aorta from 4-week-old SD rats. Stimulation of the contractile phenotype of VSMCs with TNFα significantly reduced the VSMC marker gene expression and KLF8. The gene expression of KLF8 was blocked by TNFα stimulation in an ERK-dependent manner...
January 2017: Korean Journal of Physiology & Pharmacology
https://www.readbyqxmd.com/read/28059602/sumoylation-of-klf4-promotes-il-4-induced-macrophage-m2-polarization
#9
Kezhou Wang, Wei Zhou, Qi Cai, Jinke Cheng, Rong Cai, Rong Xing
Macrophages, in response to different environmental cues, undergo the classical polarization (M1 macrophages) as well as the alternative polarization (M2 macrophages) that involve the functions of stimulus-specific transcription factors. Kruppel-like factor 4 (KLF4), a member of a subfamily of the zinc-finger class of DNA-binding transcription factors, plays as a critical regulator of macrophage polarization. KLF4 has been reported as a SUMOylated protein. In this study, we showed that SUMOylation of KLF4, is induced by IL-4 treatment in macrophages...
January 6, 2017: Cell Cycle
https://www.readbyqxmd.com/read/28052023/spermatogonial-stem-cells-and-progenitors-are-refractory-to-reprogramming-to-pluripotency-by-the-transcription-factors-oct3-4-c-myc-sox2-and-klf4
#10
Sébastien Corbineau, Bruno Lassalle, Maelle Givelet, Inès Souissi-Sarahoui, Virginie Firlej, Paul Henri Romeo, Isabelle Allemand, Lydia Riou, Pierre Fouchet
The male germinal lineage, which is defined as unipotent, produces sperm through spermatogenesis. However, embryonic primordial germ cells and postnatal spermatogonial stem cells (SSCs) can change their fate and convert to pluripotency in culture when they are not controlled by the testicular microenvironment. The mechanisms underlying these reprogramming processes are poorly understood. Testicular germ cell tumors, including teratoma, share some molecular characteristics with pluripotent cells, suggesting that cancer could result from an abnormal differentiation of primordial germ cells or from an abnormal conversion of SCCs to pluripotency in the testis...
December 28, 2016: Oncotarget
https://www.readbyqxmd.com/read/28040064/klf5-is-involved-in-regulation-of-ifitm1-2-and-3-genes-during-h5n1-virus-infection-in-a549-cells
#11
H-F Wang, L Chen, J Luo, H-X He
The interferon-induced transmembrane (IFITM) proteins usually protect cells from many virus infections by viral entry and replication. Thus, the present study aimed to identification novel regulation factors of IFITM genes. Our data showed up-regulation of IFITM1, IFITM2, and IFITM3 genes in A549 by H5N1 virus infection. However, IFITM1 was not affected, IFITM2 and IFITM3 increases expression following different concentration H5N1 virus infection in HEK293T cells. This is the first time to find differences of IFITM1 transcription in different cells with the H5N1 virus infection...
November 30, 2016: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/28028181/rybp-expression-is-regulated-by-klf4-and-sp1-and-is-related-to-hepatocellular-carcinoma-prognosis
#12
Qiaojiajie Zhao, Weihua Cai, Xuan Zhang, Shuo Tian, Junwen Zhang, Haibo Li, Congcong Hou, Xiaoli Ma, Hong Chen, Bingren Huang, Deng Chen
The expression of Ring1 and YY1 binding protein (RYBP) is reduced in several human cancers, but the molecular mechanism(s) have remained elusive. In this study, we used human HCC cell lines and tissue specimens to study the mechanism, and herein report several new findings. First, we cloned and characterized the basal promoter region of the human rybp gene. We found that the decreased RYBP expression in HCC tissues was not due to promoter sequence variation/polymorphisms or CpG dinucleotide methylation. We identified two transcription factors, KLF4 and Sp1, which directly bind the promoter region of rybp to respectively induce and suppress rybp transcription...
December 27, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28019664/pyk2-inhibition-promotes-contractile-differentiation-in-arterial-smooth-muscle
#13
Mario Grossi, Anirban Bhattachariya, Ina Nordström, Karolina M Turczyńska, Daniel Svensson, Sebastian Albinsson, Bengt-Olof Nilsson, Per Hellstrand
Modulation from contractile to synthetic phenotype of vascular smooth muscle cells is a central process in disorders involving compromised integrity of the vascular wall. Phenotype modulation has been shown to include transition from voltage-dependent towards voltage-independent regulation of the intracellular calcium level, and inhibition of non-voltage dependent calcium influx contributes to maintenance of the contractile phenotype. One possible mediator of calcium-dependent signaling is the FAK-family non-receptor protein kinase Pyk2, which is activated by a number of stimuli in a calcium-dependent manner...
December 26, 2016: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28003360/downregulation-of-insulin-receptor-substrate-1-during-hyperglycemia-induces-vascular-smooth-muscle-cell-dedifferentiation
#14
Gang Xi, Christine Wai, Morris F White, David R Clemmons
Diabetes is a major risk factor for the development of atherosclerosis but the mechanism by which hyperglycemia accelerates lesion development is not well defined. Insulin and insulin like growth factor-I (IGF-I)1 signal through the scaffold protein insulin receptor substrate-1(IRS-1). In diabetes IRS-1 is down regulated and cells become resistant to insulin. Under these conditions the IGF-I receptor signals through an alternate scaffold protein, SHPS-1, resulting in pathophysiologic stimulation of vascular smooth muscle cell (VSMC) migration and proliferation...
December 21, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27992436/transcriptomic-analysis-shows-decreased-cortical-expression-of-nr4a1-nr4a2-and-rxrb-in-schizophrenia-and-provides-evidence-for-nuclear-receptor-dysregulation
#15
Susan M Corley, Shan-Yuan Tsai, Marc R Wilkins, Cynthia Shannon Weickert
Many genes are differentially expressed in the cortex of people with schizophrenia, implicating factors that control transcription more generally. Hormone nuclear receptors dimerize to coordinate context-dependent changes in gene expression. We hypothesized that members of two families of nuclear receptors (NR4As), and retinoid receptors (RARs and RXRs), are altered in the dorsal lateral prefrontal cortex (DLPFC) of people with schizophrenia. We used next generation sequencing and then qPCR analysis to test for changes in mRNA levels for transcripts encoding nuclear receptors: orphan nuclear receptors (3 in the NR4A, 3 in the RAR, 3 in the RXR families and KLF4) in total RNA extracted from the DLPFC from people with schizophrenia compared to controls (n = 74)...
2016: PloS One
https://www.readbyqxmd.com/read/27991915/klf4-functions-as-an-activator-of-the-androgen-receptor-through-reciprocal-feedback
#16
M-K Siu, F Suau, W-Y Chen, Y-C Tsai, H-Y Tsai, H-L Yeh, Y-N Liu
In prostate cancer, Krüppel-like factor 4 (KLF4) depletion occurs frequently, suggesting a role as suppressor tumor. KLF4 is a transcription factor associated with androgen receptor (AR) expression; however, its cellular functions and signaling regulation mechanism remain largely unknown. In this study, we demonstrated that activated AR binds to the KLF4 promoter and enhances KLF4 expression, which reciprocally targets the AR promoter, thus sustaining KLF4 activity. Ectopic KLF4 expression in androgen-independent prostate cancer cells induced AR expression and decreased cell proliferation, invasion and bone metastasis...
December 19, 2016: Oncogenesis
https://www.readbyqxmd.com/read/27984723/in%C3%A2-vivo-amelioration-of-age-associated-hallmarks-by-partial-reprogramming
#17
Alejandro Ocampo, Pradeep Reddy, Paloma Martinez-Redondo, Aida Platero-Luengo, Fumiyuki Hatanaka, Tomoaki Hishida, Mo Li, David Lam, Masakazu Kurita, Ergin Beyret, Toshikazu Araoka, Eric Vazquez-Ferrer, David Donoso, Jose Luis Roman, Jinna Xu, Concepcion Rodriguez Esteban, Gabriel Nuñez, Estrella Nuñez Delicado, Josep M Campistol, Isabel Guillen, Pedro Guillen, Juan Carlos Izpisua Belmonte
Aging is the major risk factor for many human diseases. In vitro studies have demonstrated that cellular reprogramming to pluripotency reverses cellular age, but alteration of the aging process through reprogramming has not been directly demonstrated in vivo. Here, we report that partial reprogramming by short-term cyclic expression of Oct4, Sox2, Klf4, and c-Myc (OSKM) ameliorates cellular and physiological hallmarks of aging and prolongs lifespan in a mouse model of premature aging. Similarly, expression of OSKM in vivo improves recovery from metabolic disease and muscle injury in older wild-type mice...
December 15, 2016: Cell
https://www.readbyqxmd.com/read/27982015/evolution-of-multiple-cell-clones-over-a-29-year-period-of-a-cll-patient
#18
Zhikun Zhao, Lynn Goldin, Shiping Liu, Liang Wu, Weiyin Zhou, Hong Lou, Qichao Yu, Shirley X Tsang, Miaomiao Jiang, Fuqiang Li, MaryLou McMaster, Yang Li, Xinxin Lin, Zhifeng Wang, Liqin Xu, Gerald Marti, Guibo Li, Kui Wu, Meredith Yeager, Huanming Yang, Xun Xu, Stephen J Chanock, Bo Li, Yong Hou, Neil Caporaso, Michael Dean
Chronic lymphocytic leukaemia (CLL) is a frequent B-cell malignancy, characterized by recurrent somatic chromosome alterations and a low level of point mutations. Here we present single-nucleotide polymorphism microarray analyses of a single CLL patient over 29 years of observation and treatment, and transcriptome and whole-genome sequencing at selected time points. We identify chromosome alterations 13q14-, 6q- and 12q+ in early cell clones, elimination of clonal populations following therapy, and subsequent appearance of a clone containing trisomy 12 and chromosome 10 copy-neutral loss of heterogeneity that marks a major population dominant at death...
December 16, 2016: Nature Communications
https://www.readbyqxmd.com/read/27965959/a-novel-antithrombotic-mechanism-mediated-by-the-receptors-of-the-kallikrein-kinin-and-renin-angiotensin-systems
#19
REVIEW
Alvin H Schmaier
The contact activation (CAS) and kallikrein/kinin (KKS) systems regulate thrombosis risk in two ways. First, the CAS influences contact activation-induced factor XI activation and thrombin formation through the hemostatic cascade. Second, prekallikrein (PK) and bradykinin of the KKS regulate expression of three vessel wall G-protein-coupled receptors, the bradykinin B2 receptor (B2R), angiotensin receptor 2, and Mas to influence prostacyclin formation. The degree of intravascular prostacyclin formation inversely regulates intravascular thrombosis risk...
2016: Frontiers in Medicine
https://www.readbyqxmd.com/read/27934604/generation-of-induced-pluripotent-stem-cells-ipscs-stably-expressing-crispr-based-synergistic-activation-mediator-sam
#20
Kai Xiong, Yan Zhou, Poul Hyttel, Lars Bolund, Kristine Karla Freude, Yonglun Luo
Human fibroblasts were engineered to express the CRISPR-based synergistic activation mediator (SAM) complex: dCas9-VP64 and MS2-P65-HSF1. Two induced pluripotent stem cells (iPSCs) clones expressing SAM were established by transducing these fibroblasts with lentivirus expressing OCT4, SOX2, KLF4 and C-MYC. We have validated that the reprogramming cassette is silenced in the SAM iPSC clones. Expression of pluripotency genes (OCT4, SOX2, LIN28A, NANOG, GDF3, SSEA4, and TRA-1-60), differentiation potential to all three germ layers, and normal karyotypes are validated...
November 17, 2016: Stem Cell Research
keyword
keyword
11183
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"