keyword
https://read.qxmd.com/read/38535481/cell-reprogramming-and-differentiation-utilizing-messenger-rna-for-regenerative-medicine
#1
REVIEW
Masahito Inagaki
The COVID-19 pandemic generated interest in the medicinal applications of messenger RNA (mRNA). It is expected that mRNA will be applied, not only to vaccines, but also to regenerative medicine. The purity of mRNA is important for its medicinal applications. However, the current mRNA synthesis techniques exhibit problems, including the contamination of undesired 5'-uncapped mRNA and double-stranded RNA. Recently, our group developed a completely capped mRNA synthesis technology that contributes to the progress of mRNA research...
December 20, 2023: Journal of Developmental Biology
https://read.qxmd.com/read/38532151/electroacupuncture-promotes-angiogenesis-in-mice-with-cerebral-ischemia-by-inhibiting-mir-7
#2
JOURNAL ARTICLE
Qian Yu, Shi Shu, Xin-Yao Ju, Wei Peng, Xue-Qi Ren, Shu-Han Si, Shi-Zhen Song, Xue-Yun Xie, Bang-Jiang Fang, Shuang Zhou
OBJECTIVE: To observe the angiogenesis effect of electroacupuncture (EA) at Shuigou acupoint (GV 26) in the treatment of cerebral ischemia, and explore the value of miRNA-7 (miR-7) in it. METHODS: First, 48 mice were randomly divided into sham operation, middle cerebral artery occlusion (MCAO) model, and EA treatment groups. Then 9 mice were divided into carrier control group, miR-7 knockout group and miR-7 overexpression group (n=3 each group). Finally, 20 mice were divided into model and carrier control group, model and miR-7 knockout group, EA treatment and carrier control group and EA treatment and miR-7 overexpression group, with 3-6 mice in each group...
March 27, 2024: Chinese Journal of Integrative Medicine
https://read.qxmd.com/read/38531229/an-achievement-has-been-made-in-establishing-an-induced-pluripotent-stem-cell-line-sdphi005-a-from-a-healthy-chinese-male-donor
#3
JOURNAL ARTICLE
Jing Shao, Yan Jin, Chunhong Shao, Xiaoqiao Zhang, Guang Yang
Successfully transforming isolated peripheral blood mononuclear cells (PBMCs) from a healthy male into induced pluripotent stem cells (iPSCs) was achieved using non-integrating episomal vectors containing OCT4, SOX2, c-MYC, KLF4, and BCL-XL. The iPSCs exhibited a normal karyotype, expressed pluripotency markers, and demonstrated the ability to differentiate into three germ layers in vitro. This iPSC line may be employed for subsequent research purposes.
March 16, 2024: Stem Cell Research
https://read.qxmd.com/read/38509259/molecular-basis-of-cell-reprogramming-into-ipscs-with-exogenous-transcription-factors
#4
JOURNAL ARTICLE
Hisato Kondoh
A striking discovery in recent decades concerning the transcription factor (TF)-dependent process was the production of induced pluripotent stem cell (iPSCs) from fibroblasts by the exogenous expression of the TF cocktail containing Oct3/4 (Pou5f1), Sox2, Klf4, and Myc, collectively called OSKM. How fibroblast cells can be remodeled into embryonic stem cell (ESC)-like iPSCs despite high epigenetic barriers has opened a new essential avenue to understanding the action of TFs in developmental regulation. Two forerunning investigations preceded the iPSC phenomenon: exogenous TF-mediated cell remodeling driven by the action of MyoD, and the "pioneer TF" action to preopen chromatin, allowing multiple TFs to access enhancer sequences...
2024: Results and Problems in Cell Differentiation
https://read.qxmd.com/read/38509141/mirna-296-5p-promotes-the-sensitivity-of-nasopharyngeal-carcinoma-cells-to-cisplatin-via-targeted-inhibition-of-stat3-klf4-signaling-axis
#5
JOURNAL ARTICLE
Hai-Qing Luo, Yan Wang, Jing Ren, Quan-Ying Zhang, Yan Chen, Mei-Hui Chen, Ning-Xin Huang, Min-Hua Wu, Xu-Dong Tang, Xiang-Yong Li
Improving drug sensitivity is an important strategy in chemotherapy of cancer and accumulating evidence indicates that miRNAs are involved in the regulation of drug sensitivity, but the specific mechanism is still unclear. Our previous study has found that miR-296-5p was significantly downregulated in nasopharyngeal carcinoma (NPC). Here, we aim to explore whether miR-296-5p is involved in regulating cisplatin sensitivity in NPC by regulating STAT3/KLF4 signaling axis. The cell proliferation and clonogenic capacity of NPC cells were evaluated by CCK8 Assay and plate colony assay, respectively...
March 20, 2024: Scientific Reports
https://read.qxmd.com/read/38505917/identification-of-hub-genes-and-pathways-of-middle-cerebral-artery-occlusion-in-aged-rats-using-the-gene-expression-omnibus-database
#6
JOURNAL ARTICLE
Jing Guo, Yi-Zhi Yan, Jinglou Chen, Yang Duan, Peng Zeng
Stroke remained the leading cause of disability in the world, and the most important non-modifiable risk factor was age. The treatment of stroke for elder patients faced multiple difficulties due to its complicated pathogenesis and mechanism. Therefore, we aimed to identify the potential differentially expressed genes (DEGs) and singnalling pathways for aged people of stroke. To compare the DEGs in the aged rats with or without middle cerebral artery occlusion (MCAO) and to analyse the important genes and the key signaling pathways involved in the development of cerebral ischaemia in aged rats...
2024: Critical Reviews in Immunology
https://read.qxmd.com/read/38498343/circtlk1-a-novel-regulator-involved-in-vsmc-phenotypic-switching-and-the-developmental-process-of-atherosclerosis
#7
JOURNAL ARTICLE
Feng Zhang, Xuying Xiang, Cheng Wen, Xiaoqing Guo, Lei Nie, Jiaojiao Chen, Yuanpeng Xia, Bo Hu, Ling Mao
Phenotypic switching of vascular smooth muscle cells (VSMCs) is essential for atherosclerosis development. Circular RNA (circRNA) is a specific non-coding RNA that is produced as a closed-loop structure in mammals, and its specific expression pattern is closely related to its cell type and tissue. To clarify the roles of circTLK1 in VSMC phenotypic switching, we performed qRT-PCR, immunoblotting, and immunostaining. qRT-PCR revealed that circTLK1 was upregulated in both mouse models of atherosclerosis in vivo and PDGF (platelet-derived growth factor)-BB-induced VSMCs in vitro...
March 31, 2024: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://read.qxmd.com/read/38494965/-bmp9-promotes-osteogenic-differentiation-of-periodontal-ligament-stem-cells-under-inflammatory-environment-via-erk5-klf4-signaling-pathway
#8
JOURNAL ARTICLE
Li Li, Wen-Wei Lian
PURPOSE: To explore how bone morphogenetic protein 9(BMP9) promotes the osteogenic differentiation of periodontal ligament stem cells(PDLSCs) via ERK5/KLF4 signaling pathway under an inflammatory environment. METHODS: Recombinant adenovirus to overexpress BMP9 in PDLSCs stimulated with TNF-α was used and the expression level of osteogenic-related genes and proteins in BMP9-treated cells was examined using RT-PCR and Western blot. Then, KLF4 was overexpressed in PDLSCs via transfection and subjected to inflammatory stimulation to observe its effects on osteogenic differentiation and the expression of osteogenic-related genes...
December 2023: Shanghai Kou Qiang Yi Xue, Shanghai Journal of Stomatology
https://read.qxmd.com/read/38494663/microrna-7-deficiency-ameliorates-d-galactose-induced-aging-in-mice-by-regulating-senescence-of-kupffer-cells
#9
JOURNAL ARTICLE
Ya Wang, Hui Qiu, Shipeng Chen, Dongmei Li, Xu Zhao, Mengmeng Guo, Nana Li, Chao Chen, Ming Qin, Ya Zhou, Daimin Xiao, Juanjuan Zhao, Lin Xu
Aging is intricately linked to immune system dysfunction. Recent studies have highlighted the biological function of microRNA-7 (miR-7) as a novel regulator of immune cell function and related diseases. However, the potential role of miR-7 in aging remains unexplored. Here, we investigated the contribution of miR-7 to d-gal-induced aging in mice, focusing on its regulation of senescent Kupffer cells. Our findings revealed that miR-7 deficiency significantly ameliorated the aging process, characterized by enhanced CD4+ T-cell activation...
March 17, 2024: Aging Cell
https://read.qxmd.com/read/38492636/mircorna-25-3p-in-skin-precursor-cell-induced-schwann-cell-derived-extracellular-vesicles-promotes-axon-regeneration-by-targeting-tgif1
#10
JOURNAL ARTICLE
Meng Cong, Jiyu Li, Lijuan Wang, Chang Liu, Mengru Zheng, Qiang Zhou, Mingzhi Du, Xinli Ye, Min Feng, Yujiao Ye, Shuyu Zhang, Wenqing Xu, Yi Lu, Cheng Wang, Yingjie Xia, Huimin Xie, Yide Zhang, Qianru He, Leilei Gong, Yun Gu, Hualin Sun, Qi Zhang, Jian Zhao, Fei Ding, Xiaosong Gu, Songlin Zhou
Nerve injury often leads to severe dysfunction because of the lack of axon regeneration in adult mammal. Intriguingly a series of extracellular vesicles (EVs) have the obvious ability to accelerate the nerve repair. However, the detailed molecular mechanisms to describe that EVs switch neuron from a transmitter to a regenerative state have not been elucidated. This study elucidated the microRNA (miRNA) expression profiles of two types of EVs that promote nerve regeneration. The functions of these miRNAs were screened in vitro...
March 14, 2024: Experimental Neurology
https://read.qxmd.com/read/38479633/hydrogen-sulfide-improves-endothelial-barrier-function-by-modulating-the-ubiquitination-degradation-of-klf4-through-traf7-s-sulfhydration-in-diabetic-aorta
#11
JOURNAL ARTICLE
Qianzhu Li, Jiaxin Kang, Ning Liu, Jiayi Huang, Xueya Zhang, Kemiao Pang, Shiwu Zhang, Mengyi Wang, Yajun Zhao, Shiyun Dong, Hongxia Li, Dechao Zhao, Fanghao Lu, Weihua Zhang
Anomalous vascular endothelium significantly contributes to various cardiovascular diseases. VE-cadherin plays a vital role in governing the endothelial barrier. Krüppel-like factor 4(KLF4), as a transcription factor, which binds the VE-cadherin promoter and enhances its transcription. Tumor necrosis factor receptor-associated factor 7 (TRAF7) is an E3 ubiquitin ligase that has been shown to modulate the degradation of KLF4. H2 S can covalently modify cysteine residues on proteins through S-sulfhydration, thereby influencing the structure and functionality of the target protein...
March 11, 2024: Free Radical Biology & Medicine
https://read.qxmd.com/read/38472195/elevated-kallistatin-promotes-the-occurrence-and-progression-of-non-alcoholic-fatty-liver-disease
#12
JOURNAL ARTICLE
Zhenzhen Fang, Gang Shen, Yina Wang, Fuyan Hong, Xiumei Tang, Yongcheng Zeng, Ting Zhang, Huanyi Liu, Yanmei Li, Jinhong Wang, Jing Zhang, Anton Gao, Weiwei Qi, Xia Yang, Ti Zhou, Guoquan Gao
Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease worldwide, and the development of non-alcoholic steatohepatitis (NASH) might cause irreversible hepatic damage. Hyperlipidemia (HLP) is the leading risk factor for NAFLD. This study aims to illuminate the causative contributor and potential mechanism of Kallistatin (KAL) mediating HLP to NAFLD. 221 healthy control and 253 HLP subjects, 62 healthy control and 44 NAFLD subjects were enrolled. The plasma KAL was significantly elevated in HLP subjects, especially in hypertriglyceridemia (HTG) subjects, and positively correlated with liver injury...
March 12, 2024: Signal Transduction and Targeted Therapy
https://read.qxmd.com/read/38464159/kr%C3%A3-ppel-like-factor-klf-family-members-control-expression-of-genes-required-for-serous-cavity-and-alveolar-macrophage-identities
#13
Kathleen Pestal, Leianna C Slayden, Gregory M Barton
UNLABELLED: Tissue-resident macrophages adopt distinct gene expression profiles and exhibit functional specialization based on their tissue of residence. Recent studies have begun to define the signals and transcription factors that induce these identities. Here we describe an unexpected and specific role for the broadly expressed transcription factor Kruppel-like Factor 2 (KLF2) in the development of embryonically derived Large Cavity Macrophages (LCM) in the serous cavities. KLF2 not only directly regulates the transcription of genes previously shown to specify LCM identity, such as retinoic acid receptors and GATA6, but also is required for induction of many other transcripts that define the identity of these cells...
March 3, 2024: bioRxiv
https://read.qxmd.com/read/38460578/transcriptional-regulation-of-the-alternative-sex-hormone-binding-globulin-promoter-by-klf4
#14
JOURNAL ARTICLE
Warren M Meyers
In most mammals the major site of sex hormone-binding globulin (SHBG) synthesis is the liver wherefrom it is secreted into the bloodstream and is the primary determinant of sex steroid access to target tissues. The minor site of SHBG synthesis is the testis and in lower mammals testicular SHBG has long been known to be synthesized and secreted by Sertoli cells. However, human testicular SHBG is expressed in developing germ cells from an upstream alternative promoter (altP-SHBG). Transcripts arising from this region comprise an alternative first exon (1A) with the resultant protein confined to the acrosomal compartment of the mature spermatozoa...
March 7, 2024: Gene Expression Patterns: GEP
https://read.qxmd.com/read/38453894/single-cell-transcriptome-analysis-of-the-thy-tau22-mouse-model-of-alzheimer-s-disease-reveals-sex-dependent-dysregulations
#15
JOURNAL ARTICLE
Muhammad Ali, Pierre Garcia, Laetitia P Lunkes, Alessia Sciortino, Melanie Thomas, Tony Heurtaux, Kamil Grzyb, Rashi Halder, Djalil Coowar, Alex Skupin, Luc Buée, David Blum, Manuel Buttini, Enrico Glaab
Alzheimer's disease (AD) progression and pathology show pronounced sex differences, but the factors driving these remain poorly understood. To gain insights into early AD-associated molecular changes and their sex dependency for tau pathology in the cortex, we performed single-cell RNA-seq in the THY-Tau22 AD mouse model. By examining cell type-specific and cell type-agnostic AD-related gene activity changes and their sex-dimorphism for individual genes, pathways and cellular sub-networks, we identified both statistically significant alterations and interpreted the upstream mechanisms controlling them...
March 7, 2024: Cell Death Discovery
https://read.qxmd.com/read/38452705/generation-of-an-induced-pluripotent-stem-cell-line-gwcmci006-a-from-a-patient-with-autosomal-dominant-neurodevelopmental-disorder-with-or-without-hyperkinetic-movements-and-seizures-harboring-grin1-c-389a%C3%A2-%C3%A2-g-mutation
#16
JOURNAL ARTICLE
Zhen Shi, Huan Liu, Fangmei Feng, Zhifang Huang, Wen-Xiong Chen
Autosomal dominant neurodevelopmental disorder with or without hyperkinetic movements and seizures (NDHMSD) is a rare neurological disorder characterized by neurodevelopmental disorder and hyperkinetic movement, with or without seizures. Heterozygous mutation in the GRIN1 encoding the subunit 1 of the N-methyl-D-aspartate receptor caused this disorder. We first established an induced pluripotent stem cell (iPSC) line from a male patient with c.389A > G mutation in the GRIN1, via reprogramming with KLF4, SOX2, OCT3/4, and c-MYC...
February 29, 2024: Stem Cell Research
https://read.qxmd.com/read/38448665/reprogramming-of-astrocytes-to-neuronal-like-cells-in-spinal-cord-injury-a-systematic-review
#17
REVIEW
Seyed Danial Alizadeh, Mohammad-Rasoul Jalalifar, Zahra Ghodsi, Mohsen Sadeghi-Naini, Hamid Malekzadeh, Golnoosh Rahimi, Kurosh Mojtabavi, Sina Shool, Zahra Eskandari, Rasoul Masoomi, Sahar Kiani, James Harrop, Vafa Rahimi-Movaghar
STUDY DESIGN: A Systematic Review OBJECTIVES: To determine the therapeutic efficacy of in vivo reprogramming of astrocytes into neuronal-like cells in animal models of spinal cord injury (SCI). METHODS: PRISMA 2020 guidelines were utilized, and search engines Medline, Web of Science, Scopus, and Embase until June 2023 were used. Studies that examined the effects of converting astrocytes into neuron-like cells with any vector in all animal models were included, while conversion from other cells except for spinal astrocytes, chemical mechanisms to provide SCI models, brain injury population, and conversion without in-vivo experience were excluded...
March 6, 2024: Spinal Cord
https://read.qxmd.com/read/38442582/mir-29a-klf4-signaling-inhibits-breast-tumor-initiation-by-regulating-cancer-stem-cells
#18
JOURNAL ARTICLE
Qian Zhao, Yuanyuan Shang, Jinhui Lü, Yu Liu, Tao Wang, Danni Li, Jiayuan Li, Ying Lu, Zhongrui Wang, Zuoren Yu
Cancer stem cells (CSCs) are known for their potent ability to drive tumor initiation and recurrence, yet the molecular mechanisms regulating CSCs are still unclear. Our study found a positive correlation between increased levels of miR-29a and better survival rates in early-stage breast cancer patients, but a negative correlation in late-stage patients, suggesting a dual function of miR-29a in regulating breast cancer. Furthermore, miR-29a showed significant downregulation in the ALDH+ breast cancer stem cell population compared to non-stem cancer cells...
March 4, 2024: International Immunopharmacology
https://read.qxmd.com/read/38441676/transcriptome-profiling-of-longissimus-dorsi-during-different-prenatal-stages-to-identify-genes-involved-in-intramuscular-fat-deposition-in-lean-and-obese-pig-breeds
#19
JOURNAL ARTICLE
Jun Feng Chen, Jing Wang, Jin Chai, Wei Jin, Qiao Ling Ren, Qiang Ma, Qing Xia Lu, Jia Jie Sun, De Lin Mo, Jia Qing Zhang, Bao Song Xing
BACKGROUND: There was significant difference in muscle development between fat-type and lean-type pig breeds. METHODS AND RESULTS: In current study, transcriptome analysis and bioinformatics analysis were used to compare the difference in longissimus dorsi (LD) muscle at three time-points (38 days post coitus (dpc), 58 dpc, and 78 dpc ) between Huainan (HN) and Large white (LW) pig breeds. A total of 24500 transcripts were obtained in 18 samples, and 2319, 2799, and 3713 differently expressed genes (DEGs) were identified between these two breeds at 38 dpc, 58 dpc, and 78 dpc, respectively...
March 5, 2024: Molecular Biology Reports
https://read.qxmd.com/read/38438579/intestinal-epithelial-kr%C3%A3-ppel-like-factor-4-alleviates-endotoxemia-and-atherosclerosis-through-improving-nf-%C3%AE%C2%BAb-mir-34a-mediated-intestinal-permeability
#20
JOURNAL ARTICLE
He-Zhong-Rong Nie, Yi-Wen Zhou, Xiao-Hong Yu, Cong-Guo Yin, Ling-Fei Li, Hui-Qin Hao, Tao Yuan, Yong Pan
Maintenance of intestinal barrier function contributes to gastrointestinal homeostasis and therefore cardiovascular diseases. A number of studies show that intestinal permeability is affected by excessive inflammatory responses. Krüppel-like factor (KLF) 4 is one of the critical transcriptional factors, which controls multiple immune responses. In this study we investigated the role of KLF4 in regulating intestinal inflammation and permeability during the atherosclerotic process. Atherosclerotic model was established in ApoE-/- mice by feeding a high fat high cholesterol (HFHC) diet...
March 4, 2024: Acta Pharmacologica Sinica
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