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https://www.readbyqxmd.com/read/28345785/nanog-overexpression-and-its-correlation-with-stem-cell-and-differentiation-markers-in-meningiomas-of-different-who-grades
#1
Diana Freitag, Aaron Lawson McLean, Michèle Simon, Arend Koch, Susanne Grube, Jan Walter, Rolf Kalff, Christian Ewald
NANOG, as a key regulator of pluripotency and acting synergistically with other factors, has been described as a crucial transcription factor in various types of cancer. In meningiomas the expression of this marker has not yet been described. With our study, we aimed to identify and localize Nanog and other possible markers of pluripotency, stem cell properties and differentiation in meningioma tissue, to elucidate a possible effect on tumorigenesis. The gene expression levels of NANOG (NANOG1 and NANOGP8), SOX2, OCT4, KLF4, ABCG2, CMYC, MSI1, CD44, NOTCH1, NES, SALL4B, TP53 and EPAS1 were quantitatively examined using RT-qPCR in 33 surgical specimens of low- (WHO grade I) as well as in atypical and anaplastic (WHO grade II/III) meningiomas with dural tissue as reference...
March 27, 2017: Molecular Carcinogenesis
https://www.readbyqxmd.com/read/28332752/bovine-trophectoderm-cells-induced-from-bovine-fibroblasts-with-induced-pluripotent-stem-cell-reprogramming-factors
#2
Neil C Talbot, Wendy O Sparks, Caitlin Phillips, Alan D Ealy, Anne M Powell, Thomas J Caperna, Wesley M Garrett, David M Donovan, Le Ann Blomberg
Thirteen independent induced bovine trophectroderm (iBT) cell lines were established by reprogramming bovine fetal liver-derived fibroblasts after viral-vector transduction with either 6 or 8 factors, including POU5F1 (OCT4), KLF4, SOX2, MYC, NANOG, LIN28, SV40 large T antigen, and hTERT.. Light- and electron-microscopy analysis showed that the iBT cells had epithelial cell morphology typical of bovine trophectoderm cells. Reverse-transcription-PCR assays indicated that all of the cell lines expressed interferon-tau (IFNT) at passages 1 or 2...
March 23, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28331164/immortalized-prairie-vole-derived-fibroblasts-vmf-k4dts-can-be-transformed-into-pluripotent-stem-cells-and-provide-a-useful-tool-with-which-to-determine-optimal-reprogramming-conditions
#3
Masafumi Katayama, Takashi Hirayama, Tohru Kiyono, Manabu Onuma, Tetsuya Tani, Satoru Takeda, Katsuhiko Nishimori, Tomokazu Fukuda
The cellular conditions required to establish induced pluripotent stem cells (iPSCs), such as the number of reprogramming factors and/or promoter selection, differ among species. The establishment of iPSCs derived from cells of previously unstudied species therefore requires the extensive optimization of programming conditions, including promoter selection and the optimal number of reprogramming factors, through a trial-and-error approach. While the four Yamanaka factors Oct3/4, Sox2, Klf4, and c-Myc are sufficient for iPSC establishment in mice, we reported previously that six reprogramming factors were necessary for the creation of iPSCs from primary prairie vole-derived cells...
March 23, 2017: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/28324774/methylation-changes-of-sirt1-klf4-dapk1-and-spg20-in-b-lymphocytes-derived-from-follicular-and-diffuse-large-b-cell-lymphoma
#4
Raffaele Frazzi, Eleonora Zanetti, Mariaelena Pistoni, Ione Tamagnini, Riccardo Valli, Luca Braglia, Francesco Merli
Diffuse large-B cell lymphomas (DLBCL) and follicular lymphomas (FL) are the most represented subtypes among mature B-cell neoplasms and originate from malignant B lymphocytes. Methylation represents one of the major epigenetic mechanisms of gene regulation. Silent information regulator 1 (SIRT1) is a class III lysine-deacetylase playing several functions and considered to be a context-dependent tumor promoter. We present the quantitative methylation, gene expression and tissue distribution of SIRT1 and some key mediators related to lymphoma pathogenesis in B lymphocytes purified from biopsies of follicular hyperplasias, FL and DLBCL...
March 9, 2017: Leukemia Research
https://www.readbyqxmd.com/read/28323540/identification-of-multipotent-stem-cells-in-human-brain-tissue-following-stroke
#5
Kotaro Tatebayashi, Yasue Tanaka, Akiko Nakano-Doi, Rika Sakuma, Saeko Kamachi, Manabu Shirakawa, Kazutaka Uchida, Hiroto Kageyama, Toshinori Takagi, Shinichi Yoshimura, Tomohiro Matsuyama, Takayuki Nakagomi
Perivascular regions of the brain harbor multipotent stem cells. We previously demonstrated that brain pericytes near blood vessels also develop multipotency following experimental ischemia in mice, and that these ischemia-induced multipotent stem cells (iSCs) can contribute to neurogenesis. However, it is essential to understand the traits of iSCs in the post-stroke human brain for possible applications in stem cell-based therapies for stroke patients. Here we report for the first time that iSCs can be isolated from the post-stroke human brain...
March 21, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28314379/rejuvenation-by-partial-reprogramming-of-the-epigenome
#6
Andrew R Mendelsohn, James Larrick, Jennifer L Lei
Epigenetic variation with age is one of the most important hallmarks of aging. Resetting or repairing the epigenome of aging cells in intact animals may rejuvenate the cells and perhaps the entire organism. In fact, differentiated adult cells, which by definition have undergone some epigenetic changes, are capable of being rejuvenated and reprogrammed to create pluripotent stem cells and viable cloned animals. Apparently, such reprogramming is capable of completely resetting the epigenome. However, attempts to fully reprogram differentiated cells in adult animals have failed in part because reprogramming leads to formation of teratomas...
March 17, 2017: Rejuvenation Research
https://www.readbyqxmd.com/read/28314267/induced-pluripotent-stem-cell-related-genes-contribute-to-de-differentiation-in-oral-squamous-cell-carcinoma
#7
Daisuke Takeda, Takumi Hasegawa, Takeshi Ueha, Eiji Iwata, Risa Harada, Akiko Sakakibara, Teruya Kawamoto, Tsutomu Minamikawa, Yoshitada Sakai, Takahide Komori
BACKGROUND/AIM: Cancer stem cells are suspected to contribute to malignancy in tumors. Hypoxia affects cell differentiation and induces stem-cell-like characteristics in malignancies. Induced pluripotency was demonstrated in mouse fibroblasts by reprogramming with four transcriptional factors: Oct3/4, Sox2, c-Myc, and Klf4. Conversely, oncogenic transformations frequently express transcriptional factors and Nanog. Therefore, cancer cells present some similarities with induced pluripotent stem (iPS) cells...
March 2017: Anticancer Research
https://www.readbyqxmd.com/read/28304152/reprogramming-of-histone-methylation-controls-the-differentiation-of-monocytes-into-macrophages
#8
Qi-Fan Zheng, Hui-Min Wang, Zhan-Feng Wang, Jin-Yang Liu, Qi Zhang, Li Zhang, Yuan-Hua Lu, Han You, Guang-Hui Jin
Subset heterogeneity of the mononuclear phagocyte system (MPS) is controlled by defined transcriptional networks and programs; however, the dynamic establishment of programs that control broad, orchestrated expression of transcription factors (TFs) during the progression of monocyte-into-phagocyte (MP) differentiation remains largely unexplored. By using chromatin immunoprecipitation assays, we show the extensive trimethylation of histone H3 lysine 4 (H3K4me3) as well as histone H3 lysine 27 (H3K27me3) occupancy with broad footprints at the promoters of MP differentiation-related TFs, such as HOXA and FOXO genes, KLF4, IRF8 and others...
March 17, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28289824/a-mirna-signature-for-an-environmental-heterocyclic-amine-defined-by-a-multi-organ-carcinogenicity-bioassay-in-the-rat
#9
Ying-Shiuan Chen, Rong Wang, Wan-Mohaiza Dashwood, Christiane V Löhr, David E Williams, Emily Ho, Susanne Mertens-Talcott, Roderick H Dashwood
Heterocyclic amines (HCAs) produced during high-temperature cooking have been studied extensively in terms of their genotoxic/genetic effects, but recent work has implicated epigenetic mechanisms involving non-coding RNAs. Colon tumors induced in the rat by 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) have altered microRNA (miRNA) signatures linked to dysregulated pluripotency factors, such as c-Myc and Krüppel-like factor 4 (KLF4). We tested the hypothesis that dysregulated miRNAs from PhIP-induced colon tumors would provide a "PhIP signature" for use in other target organs obtained from a 1-year carcinogenicity bioassay in the rat...
March 13, 2017: Archives of Toxicology
https://www.readbyqxmd.com/read/28289232/transcriptional-landscape-of-the-human-cell-cycle
#10
Yin Liu, Sujun Chen, Su Wang, Fraser Soares, Martin Fischer, Feilong Meng, Zhou Du, Charles Lin, Clifford Meyer, James A DeCaprio, Myles Brown, X Shirley Liu, Housheng Hansen He
Steady-state gene expression across the cell cycle has been studied extensively. However, transcriptional gene regulation and the dynamics of histone modification at different cell-cycle stages are largely unknown. By applying a combination of global nuclear run-on sequencing (GRO-seq), RNA sequencing (RNA-seq), and histone-modification Chip sequencing (ChIP-seq), we depicted a comprehensive transcriptional landscape at the G0/G1, G1/S, and M phases of breast cancer MCF-7 cells. Importantly, GRO-seq and RNA-seq analysis identified different cell-cycle-regulated genes, suggesting a lag between transcription and steady-state expression during the cell cycle...
March 13, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28280847/neuronal-differentiation-capability-of-nasal-polyps-of-chronic-rhinosinusitis
#11
Michael Koennecke, Robert Böscke, Ann-Christin Pfannerstill, Stefan Reers, Martina Elsner, Benjamin Fell, Anja Richter, Karl-Ludwig Bruchhage, Sandra Schumann, Ralph Pries, Ludger Klimek, Barbara Wollenberg
Chronic rhinosinusitis with nasal polyps is considered a subgroup of chronic rhinosinusitis and a significant health problem, but the pathogenesis remains unclear to date. Therefore, we investigated the stemness to determine the role of stem cells in nasal polyps, with additional analysis of the neuronal differentiation potential of nasal polyp cells. We determined gene and protein expression profiles of stem cells in nasal polyp tissues, using whole genome microarray, quantitative real-time PCR (qPCR), immunohistochemistry, and flow cytometry...
March 9, 2017: Archivum Immunologiae et Therapiae Experimentalis
https://www.readbyqxmd.com/read/28280489/specific-and-novel-micrornas-are-regulated-as-response-to-fungal-infection-in-human-dendritic-cells
#12
Andreas Dix, Kristin Czakai, Ines Leonhardt, Karin Schäferhoff, Michael Bonin, Reinhard Guthke, Hermann Einsele, Oliver Kurzai, Jürgen Löffler, Jörg Linde
Within the last two decades, the incidence of invasive fungal infections has been significantly increased. They are characterized by high mortality rates and are often caused by Candida albicans and Aspergillus fumigatus. The increasing number of infections underlines the necessity for additional anti-fungal therapies, which require extended knowledge of gene regulations during fungal infection. MicroRNAs are regulators of important cellular processes, including the immune response. By analyzing their regulation and impact on target genes, novel therapeutic and diagnostic approaches may be developed...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28279221/notch-signaling-plays-a-crucial-role-in-cancer-stem-like-cells-maintaining-stemness-and-mediating-chemotaxis-in-renal-cell-carcinoma
#13
Wei Xiao, Zhiyong Gao, Yixing Duan, Wuxiong Yuan, Yang Ke
BACKGROUND: Cancer stem cells (CSCs) are correlated with the initiation, chemoresistance and relapse of tumors. Notch pathway has been reported to function in CSCs maintenance, but whether it is involved in renal cell carcinoma (RCC) CSCs maintaining stemness remain unclear. This study aims to explore the effect of Notch pathway on stemness of CSCs in RCC and the underlying mechanisms. METHODS: The CD133(+)/CD24(+) cells were isolated from RCC ACHN and Caki-1 cell line using Magnetic-activated cell sorting and identified by Flow cytometry analysis...
March 9, 2017: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/28278702/kr%C3%A3-ppel-like-factor-4-contributes-to-the-pathogenesis-of-mantle-cell-lymphoma
#14
Xin-Yu Li, Ling-Yun Geng, Xiang-Xiang Zhou, Na Wei, Xiao-Sheng Fang, Ying Li, Xin Wang
Mantle cell lymphoma (MCL) is an aggressive subtype of B-cell non-Hodgkin lymphoma (NHL) with poor prognosis. Krüppel-like factor 4 (KLF4) has been reported as a bi-regulator in malignancies, but little is known about its role in MCL. Here, we showed that KLF4 was downregulated in three MCL cell lines and lymph nodes from MCL patients, which resulted in a negative prognosis. We also found that the regulation of KLF4 could inhibit the proliferation and induce apoptosis of Jeko-1 cells. The lentivirally over-expressed KLF4 protein was found bind to β-catenin and could inhibit downstream molecules such as cyclinD1 and c-Myc...
February 28, 2017: Leukemia & Lymphoma
https://www.readbyqxmd.com/read/28276156/retinoic-acid-inducible-gene-1-protein-rig1-like-receptor-pathway-is-required-for-efficient-nuclear-reprogramming
#15
Nazish Sayed, Frank Ospino, Farhan Himmati, Jieun Lee, Palas Chanda, Edward S Mocarski, John P Cooke
We have revealed a critical role for innate immune signaling in nuclear reprogramming to pluripotency, and in the nuclear reprogramming required for somatic cell transdifferentiation. Activation of innate immune signaling causes global changes in the expression and activity of epigenetic modifiers to promote epigenetic plasticity. In our previous papers, we focused on the role of toll-like receptor 3 (TLR3) in this signaling pathway. Here we define the role of another innate immunity pathway known to participate in the response to viral RNA, the retinoic acid-inducible gene 1 receptor (RIG-1)-like receptor (RLR) pathway...
March 9, 2017: Stem Cells
https://www.readbyqxmd.com/read/28262547/a-role-for-klf4-in-promoting-the-metabolic-shift-via-tcl1-during-induced-pluripotent-stem-cell-generation
#16
Ken Nishimura, Shiho Aizawa, Fransiska Liliani Nugroho, Emi Shiomitsu, Yen Thi Hai Tran, Phuong Linh Bui, Evgeniia Borisova, Yuta Sakuragi, Hitomi Takada, Akira Kurisaki, Yohei Hayashi, Aya Fukuda, Mahito Nakanishi, Koji Hisatake
Reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) is accompanied by morphological, functional, and metabolic alterations before acquisition of full pluripotency. Although the genome-wide effects of the reprogramming factors on gene expression are well documented, precise mechanisms by which gene expression changes evoke phenotypic responses remain to be determined. We used a Sendai virus-based system that permits reprogramming to progress in a strictly KLF4-dependent manner to screen for KLF4 target genes that are critical for the progression of reprogramming...
March 14, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28256619/a-core-regulatory-circuit-in-glioblastoma-stem-cells-links-mapk-activation-to-a-transcriptional-program-of-neural-stem-cell-identity
#17
Gregory Riddick, Svetlana Kotliarova, Virginia Rodriguez, H S Kim, Amanda Linkous, Andrew J Storaska, Susie Ahn, Jennifer Walling, Galina Belova, Howard A Fine
Glioblastoma, the most common primary malignant brain tumor, harbors a small population of tumor initiating cells (glioblastoma stem cells) that have many properties similar to neural stem cells. To investigate common regulatory networks in both neural and glioblastoma stem cells, we subjected both cell types to in-vitro differentiation conditions and measured global gene-expression changes using gene expression microarrays. Analysis of enriched transcription factor DNA-binding sites in the promoters of differentially expressed genes was used to reconstruct regulatory networks involved in differentiation...
March 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28247282/backbone-1-h-13-c-and-15-n-nmr-resonance-assignments-of-the-kr%C3%A3-ppel-like-factor-4-activation-domain
#18
Brigid S Conroy, Emma R Weiss, Steven P Smith, David N Langelaan
Krüppel-like factor 4 (KLF4) is a transcription factor involved in diverse biological processes, including development, cellular differentiation and proliferation, and maintenance of tissue homeostasis. KLF4 has also been associated with disease states, such as cardiovascular disease and several cancers. KLF4 contains an activation domain, repression domain, and a structurally characterized C-terminal zinc finger domain that mediates its binding to DNA. The structurally uncharacterized KLF4 activation domain is critical for transactivation by KLF4 and mediates its binding to the transcriptional coactivator CBP/p300...
February 28, 2017: Biomolecular NMR Assignments
https://www.readbyqxmd.com/read/28240438/feeder-independent-canine-induced-pluripotent-stem-cells-maintained-under-serum-free-conditions
#19
Toshiya Nishimura, Shingo Hatoya, Ryoji Kanegi, Daluthgamage Pasty Himali Wijesekera, Kousuke Sanno, Erina Tanaka, Kikuya Sugiura, Noritoshi Kawate, Hiromitsu Tamada, Hiroshi Imai, Toshio Inaba
Canine induced pluripotent stem cells (ciPSCs) are an attractive source for regenerative veterinary medicine, and may also serve as a disease model for human regenerative medicine. Extending the application of ciPSCs from bench to bedside, however, requires the resolution of many issues. We generated ciPSCs expressing doxycycline-inducible murine Oct3/4 (Pou5f1), Sox2, Klf4, and c-Myc, which were introduced using lentiviral vectors. The resultant ciPSCs required doxycycline to proliferate in the undifferentiated state...
February 27, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28240320/analysis-of-co-associated-transcription-factors-via-ordered-adjacency-differences-on-motif-distribution
#20
Gaofeng Pan, Jijun Tang, Fei Guo
Transcription factors (TFs) binding to specific DNA sequences or motifs, are elementary to the regulation of transcription. The gene is regulated by a combination of TFs in close proximity. Analysis of co-TFs is an important problem in understanding the mechanism of transcriptional regulation. Recently, ChIP-seq in mapping TF provides a large amount of experimental data to analyze co-TFs. Several studies show that if two TFs are co-associated, the relative distance between TFs exhibits a peak-like distribution...
February 27, 2017: Scientific Reports
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