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membrane fluidity

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https://www.readbyqxmd.com/read/28427058/dietary-selenium-or-zinc-supplementation-restores-brain-lipid-composition-and-membrane-fluidity-in-protein-undernourished-rats
#1
Olusegun L Adebayo, Bamidele A Salau, Rajat Sandhir, Gbenga A Adenuga
Studies have shown that protein undernutrition (PU) modifies the membrane lipid composition in the intestine and liver, as well as in plasma and other areas. However, there is limited information on the effect of PU on synaptosomal membrane lipid composition and fluidity and the protective role of selenium (Se) and zinc (Zn), which is a major focus of the present study. For 10 weeks, rats were fed diets containing 16% casein, which constituted the adequate protein diet, or 5% casein, representing the PU diet...
April 21, 2017: Developmental Neuroscience
https://www.readbyqxmd.com/read/28417231/high-cholesterol-low-cholesterol-effects-in-biological-membranes-review
#2
Witold K Subczynski, Marta Pasenkiewicz-Gierula, Justyna Widomska, Laxman Mainali, Marija Raguz
Lipid composition determines membrane properties, and cholesterol plays a major role in this determination as it regulates membrane fluidity and permeability, as well as induces the formation of coexisting phases and domains in the membrane. Biological membranes display a very diverse lipid composition, the lateral organization of which plays a crucial role in regulating a variety of membrane functions. We hypothesize that, during biological evolution, membranes with a particular cholesterol content were selected to perform certain functions in the cells of eukaryotic organisms...
April 17, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28413858/epr-studies-on-the-properties-of-model-photoreceptor-membranes-made-of-natural-and-synthetic-lipids
#3
Mariusz Duda, Katarzyna Kawula, Anna Pawlak, Tadeusz Sarna, Anna Wisniewska-Becker
The membranes of retina photoreceptors have unique lipid composition. They contain a high concentration of polyunsaturated docosahexaenoic acid, with six double bonds, and are enriched in phosphatidylethanolamines. Based on their phospholipid composition and cholesterol content, membranes of photoreceptors can be divided into three types: plasma membrane, young disks membranes, and old disks membranes. High amount of docosahexaenoic acid, abundant illumination, and high respiratory demands make these membranes sensitive to oxidative stress and lipid peroxidation...
April 17, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28411183/membrane-permeation-of-arginine-rich-cell-penetrating-peptides-independent-of-transmembrane-potential-as-a-function-of-lipid-composition-and-membrane-fluidity
#4
Rike Wallbrecher, Tobias Ackels, R Alis Olea, Marco J Klein, Lucie Caillon, Jürgen Schiller, Petra H Bovée-Geurts, Toin H van Kuppevelt, Anne S Ulrich, Marc Spehr, Merel J W Adjobo-Hermans, Roland Brock
Cell-penetrating peptides (CPPs) are prominent delivery vehicles to confer cellular entry of (bio-) macromolecules. Internalization efficiency and uptake mechanism depend, next to the type of CPP and cargo, also on cell type. Direct penetration of the plasma membrane is the preferred route of entry as this circumvents endolysosomal sequestration. However, the molecular parameters underlying this import mechanism are still poorly defined. Here, we make use of the frequently used HeLa and HEK cell lines to address the role of lipid composition and membrane potential...
April 11, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28410994/the-role-of-oxysterols-in-human-cancer
#5
REVIEW
Alzbeta Kloudova, F Peter Guengerich, Pavel Soucek
Oxysterols are oxygenated derivatives of cholesterol formed in the human body or ingested in the diet. By modulating the activity of many proteins [e.g., liver X receptors (LXRs), oxysterol-binding proteins (OSBPs), some ATP-binding cassette (ABC) transporters], oxysterols can affect many cellular functions and influence various physiological processes (e.g., cholesterol metabolism, membrane fluidity regulation, intracellular signaling pathways). Therefore, the role of oxysterols is also important in pathological conditions (e...
April 12, 2017: Trends in Endocrinology and Metabolism: TEM
https://www.readbyqxmd.com/read/28409384/microbial-response-to-environmental-stresses-from-fundamental-mechanisms-to-practical-applications
#6
REVIEW
Ningzi Guan, Jianghua Li, Hyun-Dong Shin, Guocheng Du, Jian Chen, Long Liu
Environmental stresses are usually active during the process of microbial fermentation and have significant influence on microbial physiology. Microorganisms have developed a series of strategies to resist environmental stresses. For instance, they maintain the integrity and fluidity of cell membranes by modulating their structure and composition, and the permeability and activities of transporters are adjusted to control nutrient transport and ion exchange. Certain transcription factors are activated to enhance gene expression, and specific signal transduction pathways are induced to adapt to environmental changes...
April 13, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28409218/transmembrane-helices-containing-a-charged-arginine-are-thermodynamically-stable
#7
Martin B Ulmschneider, Jakob P Ulmschneider, J Alfredo Freites, Gunnar von Heijne, Douglas J Tobias, Stephen H White
Hydrophobic amino acids are abundant in transmembrane (TM) helices of membrane proteins. Charged residues are sparse, apparently due to the unfavorable energetic cost of partitioning charges into nonpolar phases. Nevertheless, conserved arginine residues within TM helices regulate vital functions, such as ion channel voltage gating and integrin receptor inactivation. The energetic cost of arginine in various positions along hydrophobic helices has been controversial. Potential of mean force (PMF) calculations from atomistic molecular dynamics simulations predict very large energetic penalties, while in vitro experiments with Sec61 translocons indicate much smaller penalties, even for arginine in the center of hydrophobic TM helices...
April 13, 2017: European Biophysics Journal: EBJ
https://www.readbyqxmd.com/read/28408132/comparison-of-the-effects-of-major-fatty-acids-present-in-the-mediterranean-diet-oleic-acid-docosahexaenoic-acid-and-in-hydrogenated-oils-elaidic-acid-on-7-ketocholesterol-induced-oxiapoptophagy-in-microglial-bv-2-cells
#8
Meryam Debbabi, Amira Zarrouk, Maryem Bezine, Wiem Meddeb, Thomas Nury, Asmaa Badreddine, El Mostafa Karym, Randa Sghaier, Lionel Bretillon, Stéphane Guyot, Mohammad Samadi, Mustapha Cherkaoui-Malki, Boubker Nasser, Mondher Mejri, Sofien Ben-Hammou, Mohamed Hammami, Gérard Lizard
Increased levels of 7-ketocholesterol (7KC), which results mainly from cholesterol auto-oxidation, are often found in the plasma and/or cerebrospinal fluid of patients with neurodegenerative diseases and might contribute to activation of microglial cells involved in neurodegeneration. As major cellular dysfunctions are induced by 7KC, it is important to identify molecules able to impair its side effects. Since consumption of olive and argan oils, and fish is important in the Mediterranean diet, the aim of the study was to determine the ability of oleic acid (OA), a major compound of olive and argan oil, and docosahexaenoic acid (DHA) present in fatty fishes, such as sardines, to attenuate 7KC-induced cytotoxic effects...
April 10, 2017: Chemistry and Physics of Lipids
https://www.readbyqxmd.com/read/28405720/ceramide-synthase-2-deficiency-aggravates-aom-dss-induced-colitis-in-mice-role-of-colon-barrier-integrity
#9
Stephanie Oertel, Klaus Scholich, Andreas Weigert, Dominique Thomas, Julia Schmetzer, Sandra Trautmann, Marthe-Susanna Wegner, Heinfried H Radeke, Natalie Filmann, Bernhard Brüne, Gerd Geisslinger, Irmgard Tegeder, Sabine Grösch
Loss of intestinal barrier functions is a hallmark of inflammatory bowel disease like ulcerative colitis. The molecular mechanisms are not well understood, but likely involve dysregulation of membrane composition, fluidity, and permeability, which are all essentially regulated by sphingolipids, including ceramides of different chain length and saturation. Here, we used a loss-of-function model (CerS2(+/+) and CerS2(-/-) mice) to investigate the impact of ceramide synthase 2, a key enzyme in the generation of very long-chain ceramides, in the dextran sodium salt (DSS) evoked model of UC...
April 12, 2017: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/28402890/cholesterol-regulates-monocyte-rolling-through-cd44-distribution
#10
Amit K Saha, Pawel Osmulski, Shatha F Dallo, Maria Gaczynska, Tim H-M Huang, Anand K Ramasubramanian
Cholesterol is an important risk factor of atherosclerosis, due to its active uptake by monocytes/macrophages. Monocyte recruitment from flowing blood to atherosclerotic foci is the key first step in the development of atherosclerosis. Cholesterol content alters cell membrane stiffness, and lateral lipid and protein diffusion. We hypothesized that cholesterol content will modulate the recruitment of monocytes to inflamed endothelial surface by altering the dynamics of adhesion receptors. We depleted or enriched the cellular cholesterol levels using methyl-β-cyclodextran in freshly isolated human monocytes...
April 11, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28401504/metabolism-and-secretion-of-yellow-pigment-under-high-glucose-stress-with-monascus-ruber
#11
Tao Huang, Meihua Wang, Kan Shi, Gong Chen, Xiaofei Tian, Zhenqiang Wu
The biosynthesis of microbial secondary metabolites is induced by a wide range of environmental stresses. In this study, submerged fermentation of Monascus yellow pigments by Monascus ruber CGMCC 10910 under high glucose stress was investigated. The increase of lipid content was the major contributor to the increase of dry cell weight (DCW), and the lipid-free DCW was only slightly changed under high glucose stress, which benefited the accumulation of intracellular hydrophobic pigments. The fatty acid composition analysis in Monascus cell membranes showed that high glucose stress significantly increased the ratio of unsaturated/saturated fatty acid and the index of unsaturated fatty acid (IUFA) value, which would improve the fluidity and permeability of the cell membrane...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28389697/antioxidant-capacity-lipid-peroxidation-and-lipid-composition-changes-during-long-term-and-short-term-thermal-acclimation-in-daphnia
#12
Bret L Coggins, John W Collins, Kailea J Holbrook, Lev Y Yampolsky
Examples of phenotypic plasticity-the ability of organisms of identical genotypes to produce different phenotypes in response to the environment-are abundant, but often lack data on the causative physiology and biochemistry. Phenotypes associated with increased protection against or reduced damage from harmful environments may, in fact, be downstream effects of hidden adaptive responses that remain elusive to experimental measurement or be obscured by homeostatic or over-compensatory effects. The freshwater zooplankton crustacean Daphnia drastically increases its heat tolerance as the result of acclimation to high temperatures, an effect often assumed to be based on plastic responses allowing better protection against oxidative stress...
April 7, 2017: Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology
https://www.readbyqxmd.com/read/28389203/preferential-binding-of-inorganic-mercury-to-specific-lipid-classes-and-its-competition-with-cadmium
#13
Evan Kerek, Mohamed Hassanin, Wenzhou Zhang, Elmar J Prenner
Upon uptake of Hg and Cd into living systems, possible targets for metal induced toxicity include the membranes surrounding nervous, cardiovascular and renal cells. To further our understanding of the interactions of Hg and Cd with different lipid structures under physiologically relevant chloride and pH conditions (100 mM NaCl pH 7.4), we used fluorescence spectroscopy and dynamic light scattering to monitor changes in membrane fluidity and phase transition and liposome size. The metal effects were studied on zwitterionic, cationic and anionic lipids to elucidate electrostatically driven metal-lipid interactions...
April 4, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28375583/flow-cytometry-and-capillary-electrophoresis-analyses-in-ethanol-stressed-oenococcus-oeni-strains-and-changes-assessment-of-membrane-fatty-acids-composition
#14
M G Bonomo, C Cafaro, A Guerrieri, F Crispo, L Milella, L Calabrone, G Salzano
AIMS: This study aimed to investigate dynamics and physiological heterogeneity of Oenococcus oeni under different conditions, cell membrane fluidity and permeability variations and assessment of changes in cell surface charging rates. METHODS AND RESULTS: Flow cytometry, membrane fatty acid analysis and capillary electrophoresis were performed to study ethanol-induced variations. Different physiological states were assessed, revealing cell subpopulations able to adapt and withstand to environmental stress, in order to recover their functionality...
April 4, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28371558/dynamic-cellular-interactions-with-extracellular-matrix-triggered-by-biomechanical-tuning-of-low-rigidity-supported-lipid-membranes
#15
Setareh Vafaei, Seyed R Tabaei, Kabir H Biswas, Jay T Groves, Nam-Joon Cho
The behavior of cells in a tissue is regulated by chemical as well as physical signals arising from their microenvironment. While gel-based substrates have been widely used for mimicking a range of substrate rigidities, there is a need for the development of low rigidity substrates for mimicking the physical properties of soft tissues. In this study, the authors report the development of a supported lipid bilayer (SLB)-based low rigidity substrate for cell adhesion studies. SLBs are functionalized with either collagen I or fibronectin via covalent, amine coupling to a carboxyl group-modified lipid molecule...
March 30, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28363597/the-cytotoxic-effects-of-ve-3n-a-novel-1-4-dihydropyridine-derivative-involve-the-mitochondrial-bioenergetic-disruption-via-uncoupling-mechanisms
#16
Javier Marín-Prida, Gilberto L Pardo Andreu, Camila Pederiva Rossignoli, Michael González Durruthy, Estael Ochoa Rodríguez, Yamila Verdecia Reyes, Roberto Fernández Acosta, Sergio A Uyemura, Luciane C Alberici
Several 1,4-dihydropyridine derivatives overcome the multidrug resistance in tumors, but their intrinsic cytotoxic mechanisms remain unclear. Here we addressed if mitochondria are involved in the cytotoxicity of the novel 1,4-dihydropyridine derivative VE-3N [ethyl 6-chloro-5-formyl-2-methyl-4-(3-nitrophenyl)-1,4-dihydropyridine-3-carboxylate] towards cancer cells by employing hepatic carcinoma (HepG2) cells and isolated rat liver mitochondria. In HepG2 cells, VE-3N induced mitochondrial membrane potential dissipation, ATP depletion, annexin V/propidium iodide double labeling, and Hoechst staining; events indicating apoptosis induction...
March 29, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28353350/multi-level-characterization-of-the-membrane-properties-of-resveratrol-incorporated-liposomes
#17
Jin Han, Keishi Suga, Keita Hayashi, Yukihiro Okamoto, Hiroshi Umakoshi
Resveratrol (RES) is a kind of polyphenolic compounds discovered from grapes and has become a prominence as a possible contributor to many diseases treatments. Herein, three different types of liposomes were prepared as model cell membranes, and then the influence of the incorporation of RES on their membrane properties was evaluated by utilizing the membrane-binding fluorescent probes. The binding of RES lead the membrane polarities decreased slightly, regardless of the phase states of membrane. While the membrane fluidities decreased only in the case of liquid-disordered phase...
March 29, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28349837/apoptosis-mediated-chemosensitization-of-tumor-cells-to-5-fluorouracil-on-supplementation-of-fish-oil-in-experimental-colon-carcinoma
#18
Isha Rani, Bhoomika Sharma, Sandeep Kumar, Satinder Kaur, Navneet Agnihotri
5-Fluorouracil has been considered as a cornerstone therapy for colorectal cancer; however, it suffers from low therapeutic response rate and severe side effects. Therefore, there is an urgent need to increase the clinical efficacy of 5-fluorouracil. Recently, fish oil rich in n-3 polyunsaturated fatty acids has been reported to chemosensitize tumor cells to anti-cancer drugs. This study is designed to understand the underlying mechanisms of synergistic effect of fish oil and 5-fluorouracil by evaluation of tumor cell-associated markers such as apoptosis and DNA damage...
March 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28349093/regulation-of-thermotrps-by-lipids
#19
REVIEW
Sara L Morales-Lázaro, Luis Lemus, Tamara Rosenbaum
The family of Transient Receptor Potential (TRP) ion channels is constituted by 7 subfamilies among which are those that respond to temperature, the thermoTRPs. These channels are versatile molecules of a polymodal nature that have been shown to be modulated in various fashions by molecules of a lipidic nature. Some of these molecules interact directly with the channels on specific regions of their structures and some of these promote changes in membrane fluidity or modify their gating properties in response to their agonists...
2017: Temperature: Multidisciplinary Biomedical Journal
https://www.readbyqxmd.com/read/28348085/the-extent-of-the-temperature-induced-membrane-remodeling-in-two-closely-related-bordetella-species-reflects-their-adaptation-to-diverse-environmental-niches
#20
Gabriela Seydlova, Jana Beranova, Ilona Bibova, Ana Dienstbier, Jakub Drzmisek, Jiri Masin, Radovan Fiser, Ivo Konopasek, Branislav Vecerek
Changes in environmental temperature represent one of the major stresses faced by microorganisms as it affects the function of the cytoplasmic membrane. In this study we have analyzed the thermal adaptation in two closely related respiratory pathogens Bordetella pertussis and Bordetella bronchiseptica. While B. pertussis represents a pathogen strictly adapted to the human body temperature, B. bronchiseptica causes infection in a broad range of animals and survives also outside of the host. We applied GC-MS to determine the fatty acids of both Bordetella species grown at different temperatures and analyzed the membrane fluidity by fluorescence anisotropy measurement...
March 27, 2017: Journal of Biological Chemistry
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