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membrane fluidity

Henriette S Frislev, Janni Nielsen, Jesper Nylandsted, Daniel Otzen
Cholesterol (chol) is important in all mammalian cells as a modulator of membrane fluidity. However, its low solubility is a challenge for controlled delivery to membranes. Here we introduce a new tool to deliver chol to membranes, namely, liprotides, i.e., protein-lipid complexes composed of a fatty acid core decorated with partially denatured protein. We focus on liprotides prepared by incubating Ca2+ -depleted α-lactalbumin with oleic acid (OA) for 1 h at 20 °C (lip20) or 80 °C (lip80). The binding and membrane delivery properties of liprotides is compared to the widely chol transporter methyl-β-cyclodextrin (mBCD)...
April 17, 2018: Journal of Membrane Biology
Daichi Yamaura, Daisuke Tadaki, Shun Araki, Miyu Yoshida, Kohei Arata, Takeshi Ohori, Ken-Ichi Ishibashi, Miki Kato, Teng Ma, Ryusuke Miyata, Hideaki Yamamoto, Ryugo Tero, Masao Sakuraba, Toshio Ogino, Michio Niwano, Ayumi Hirano-Iwata
Artificial bilayer lipid membranes (BLMs) provide well-defined systems for investigating the fundamental properties of membrane proteins, including ion channels, and in screening the effect of drugs that act on them. However, the application of this technique is limited due to the low stability and low reconstitution efficiency of the process. We previously reported on improving the stability of BLM based on the fabrication of microapertures having a tapered edge in SiO2/Si3N4 septa and efficient ion channel incorporation based on vesicle fusion accelerated by a centrifugal force...
April 17, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
Waldemar Seel, Alexander Flegler, Marija Zunabovic-Pichler, André Lipski
Listeria monocytogenes is a food pathogen capable of growing at a broad temperature range from 50°C to refrigerator temperatures. A key requirement for bacterial activity and growth at low temperatures is the ability to adjust membrane lipid composition to maintain cytoplasma membrane fluidity. In this study we confirmed earlier findings that the extent of fatty acid profile adaptation differed between L. monocytogenes strains. We could demonstrate for isolates from food that growth rates at low temperatures and resistance against freeze-thaw stress was not impaired by a lower adaptive response of fatty acid composition...
April 16, 2018: Journal of Bacteriology
Mark L Schultz, Luis Tecedor, Elena Lysenko, Shyam Ramachandran, Colleen S Stein, Beverly L Davidson
The neuronal ceroid lipofuscinoses are a class of inherited neurodegenerative diseases characterized by the accumulation of autofluorescent storage material. The most common neuronal ceroid lipofuscinosis has juvenile onset with rapid onset blindness and progressive degeneration of cognitive processes. The juvenile form is caused by mutations in the CLN3 gene, which encodes the protein CLN3. While mouse models of Cln3 deficiency show mild disease phenotypes, it is apparent from patient tissue- and cell-based studies that its loss impacts many cellular processes...
April 13, 2018: Neurobiology of Disease
Marzena Mach, Karolina Węder, Katarzyna Hąc-Wydro, Michał Flasiński, Joanna Lewandowska-Lancucka, Kinga Wójcik, Pawel Wydro
An increasing number of bacterial infections and the rise in antibiotic resistance of a number of bacteria speciesforces to search for new antibacterial compounds. The latter facts motivate the investigations presented herein and aimed at studying the influence of a cationic lipid:1-palmitoyl-2-oleoyl-sn-glycero-3-ethylphosphocholine (EPOPC) on model (mono,- and bilayer) membranes. The monolayer experiments involved the analysis of the interactions of EPOPC with bacterial membrane lipids in one component and mixed systems as well as Brewster Angle Microcopy studies...
April 13, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
Sara Sameni, Leonel Malacrida, Zhiqun Tan, Michelle A Digman
A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper.
April 11, 2018: Scientific Reports
Asha Bhujangrao Shiradhone, Sujata S Ingle, And Gajanan B Zore
Considering the significance in survival and virulence, we have made an attempt to understand modulations in the membrane and cell wall properties of Candida albicans hyphae induced by temperature (37 °C) and neutral pH and yeast form cells grown under low hydrostatic pressure (LHP). Our results suggest that cell surface hydrophobicity (CSH) and adhesion are dynamic properties determined largely by the microenvironment rather than morphological forms, citing the significance of variation in niche specific virulence...
April 6, 2018: Journal of Fungi (Basel, Switzerland)
Jordan D Poley, Laura M Braden, Amber M Messmer, Okechukwu O Igboeli, Shona K Whyte, Alicia Macdonald, Jose Rodriguez, Marta Gameiro, Lucien Rufener, Jacques Bouvier, Dorota W Wadowska, Ben F Koop, Barry C Hosking, Mark D Fast
Drug resistance in the salmon louse Lepeophtheirus salmonis is a global issue for Atlantic salmon aquaculture. Multiple resistance has been described across most available compound classes with the exception of the benzoylureas. To target this gap in effective management of L. salmonis and other species of sea lice (e.g. Caligus spp.), Elanco Animal Health is developing an in-feed treatment containing lufenuron (a benzoylurea) to be administered prior to seawater transfer of salmon smolts and to provide long-term protection of salmon against sea lice infestations...
March 13, 2018: International Journal for Parasitology, Drugs and Drug Resistance
Katarzyna Dopierała, Marta Skrzypiec
The aim of the present study was to investigate the interfacial behaviour of model biomembranes in the presence of β-carotene (βC). The Langmuir monolayer technique was used to form the mixed lipid film at the air/water interface. Using the surface pressure-area isotherms, the surface potential-area curves and the Brewster angle microscopy the nature of interactions between carotenoid and lipid components of the monolayers was investigated. The results were obtained for complex models of the lipid bilayer composed of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and cholesterol (CHOL)...
April 4, 2018: Chemistry and Physics of Lipids
Chiharu Mizuguchi, Mitsuki Nakamura, Naoko Kurimitsu, Takashi Ohgita, Kazuchika Nishitsuji, Teruhiko Baba, Akira Shigenaga, Toshinori Shimanouchi, Keiichiro Okuhira, Akira Otaka, Hiroyuki Saito
Here, we examined the effects of phosphatidylserine (PS) and cholesterol on the fibril-forming properties of the N-terminal 1‒83 fragment of an amyloidogenic G26R variant of apoA-I bound to small unilamellar vesicles. A thioflavin T fluorescence assay together with microscopic observations showed that PS significantly retards the nucleation step in fibril formation by apoA-I 1‒83/G26R, whereas cholesterol slightly enhances fibril formation. Circular dichroism analyses demonstrated that PS facilitates a structural transition from random coil to α-helix in apoA-I 1‒83/G26R with great stabilization of the α-helical structure upon lipid binding...
April 3, 2018: Scientific Reports
Jihee Sohn, Hang Lin, Madalyn Rose Fritch, Rocky S Tuan
BACKGROUND: Adult mesenchymal stem cells (MSCs) are an important resource for tissue growth, repair, and regeneration. To utilize MSCs more effectively, a clear understanding of how they react to environmental cues is essential. Currently, relatively little is known about how the composition of the plasma membranes affects stem cell phenotype and properties. The presence of lipid molecules, including cholesterol in particular, in the plasma membrane plays a crucial role in regulating a variety of physiological processes in cells...
April 3, 2018: Stem Cell Research & Therapy
Abdullah A Saber, Karolina Fučíková, Hilary A McManus, Graziano Guella, Marco Cantonati
The biodiversity of terrestrial algae is still grossly understudied, and African deserts in particular are barely touched in this respect. Here, four coccoid green algae from oases in the Western Desert of Egypt were characterized using a combination of morphotaxonomic, ecological and 18S rDNA data, with additional carotenoid and lipid analyses for two of the strains. Three strains were identified as affiliated with known taxa: Mychonastes sp., Asterarcys sp. (first report of this genus from a desert soil), and Stichococcus cf...
March 30, 2018: Journal of Phycology
Lin Zhang, Ting Wang, Shou-Ying Du, Yang Lu, Zhi-Heng Fan, Jun-Ming Ma, Jia-Wei Tan, Yu-Tao Xue
The aim of this research is to investigate the effects of paeoniflorin and menthol on the physiological function of Calu-3 cell membrane during the transport of puerarin. Calu-3 cell was used as the in vitro cell model to simulate nasal mucosa tissues, and the cell membrane fluidity, Na⁺-K⁺-ATPase activity and Ca²⁺-ATPase activity were detected by fluorescence recovery after photobleaching(FRAP) and ultramicro enzyme activity testing, in order to explore the mechanism of compatible drugs on promoting puerarin transport...
February 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Venkatraman Srinivasan Radhakrishnan, Surya Prakash Dwivedi, Mohammed Haris Siddiqui, Tulika Prasad
Silver nanoparticles (AgNps) have attracted maximal attention among all metal nanoparticles, and the study of their biological properties has gained impetus for further medical adoption. This study evaluated the cellular and molecular mechanisms associated with the action of AgNps against an opportunistic pathogen, Candida albicans . Spherical, stable AgNp (average size 21.6 nm) prepared by a chemical reduction method showed minimum inhibitory concentration (required to inhibit the growth of 90% of organisms) at 40 μg/mL...
2018: International Journal of Nanomedicine
Dharshini Selvarajan, Chakravarthi Mohan, Vignesh Dhandapani, Gauri Nerkar, Ashwin Narayan Jayanarayanan, Manoj Vadakkancherry Mohanan, Naveenarani Murugan, Lovejot Kaur, Mahadevaiah Chennappa, Ravinder Kumar, Minturam Meena, Bakshi Ram, Appunu Chinnaswamy
Sugarcane ( Saccharum sp.) is predominantly grown in both tropics and subtropics in India, and the subtropics alone contribute more than half of sugarcane production. Sugarcane active growth period in subtropics is restricted to 8-9 months mainly due to winter's low temperature stress prevailing during November to February every year. Being a commercial crop, tolerance to low temperature is important in sugarcane improvement programs. Development of cold tolerant sugarcane varieties require a deep knowledge on molecular mechanism naturally adapted by cold tolerant genotypes during low temperature stress...
April 2018: 3 Biotech
Mohit P Mathew, Julie G Donaldson
Clathrin-independent endocytosis (CIE) is a form of endocytosis that lacks a defined cytoplasmic machinery. Here, we asked whether glycan interactions, acting from the outside, could be a part of that endocytic machinery. We show that the perturbation of global cellular patterns of protein glycosylation by modulation of metabolic flux affects CIE. Interestingly, these changes in glycosylation had cargo-specific effects. For example, in HeLa cells, N-acetylglucosamine treatment, which increases glycan branching, increased major histocompatibility complex class I (MHCI) internalization but inhibited CIE of the glycoprotein CD59 molecule (CD59)...
March 26, 2018: Journal of Biological Chemistry
Devanita Ghosh, Punyasloke Bhadury, Joyanto Routh
Elevated levels of arsenic (As) in aquifers of South East Asia have caused diverse health problems affecting millions of people who drink As-rich groundwater and consume various contaminated agriculture products. The biogeochemical cycling and mobilization/immobilization of As from its mineral-bound phase is controlled by pH, oxic/anoxic conditions, and different microbial processes. The increased As flux generated from ongoing biogeochemical processes in the subsurface in turn affects the in situ microbial communities...
March 25, 2018: MicrobiologyOpen
Kamila Pasternak, Dominika Wróbel, Olga Nowacka, Ireneusz Pieszyński, Maria Bryszewska, Jolanta Kujawa
INTRODUCTION: Authors of numerous publications have proved the therapeutic effect of laser irradiation on biological material, but the mechanisms at cellular and subcellular level are not yet well understood. OBJECTIVE: The aim of this study was to assess the effect of laser radiation emitted by the MLS M1 system (Multiwave Locked System) at two wavelengths (808 nm continuous and 905 nm pulsed) on the stability and fluidity of liposomes with a lipid composition similar to that of human erythrocyte membrane or made of phosphatidylocholine...
March 14, 2018: Annals of Agricultural and Environmental Medicine: AAEM
Md Soriful Islam, Clara Castellucci, Rosamaria Fiorini, Stefania Greco, Riccardo Gagliardi, Alessandro Zannotti, Stefano R Giannubilo, Andrea Ciavattini, Natale G Frega, Deborah Pacetti, Pasquapina Ciarmela
Uterine leiomyomas (fibroids or myomas) are the most common benign tumors of premenopausal women and new medical treatments are needed. This study aimed to determine the effects of omega-3 fatty acids on the lipid profile, membrane architecture and gene expression patterns of extracellular matrix components (collagen1A1, fibronectin, versican, or activin A), mechanical signaling (integrin β1, FAK, and AKAP13), sterol regulatory molecules (ABCG1, ABCA1, CAV1, and SREBF2), and mitochondrial enzyme (CYP11A1) in myometrial and leiomyoma cells...
March 25, 2018: Journal of Cellular Physiology
M Togo, N Konari, M Tsukamoto, R Kimoto, T Yamaguchi, H Takeda, I Kambayashi
Background: Obesity is a primary factor of lifestyle-related diseases, and the age of its onset has decreased. The reactive oxygen species (ROS), the superoxide anion, is generated in the mitochondrial electron transport chain and the damage it induces in cells may be a contributing factor to obesity-related lifestyle diseases. In the present study, the influence of the ingestion of a high-fat diet (HFD) on superoxide anion generation in rat liver mitochondria (Mt) and membrane fluidity was investigated...
2018: Journal of the International Society of Sports Nutrition
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