keyword
MENU ▼
Read by QxMD icon Read
search

membrane fluidity

keyword
https://www.readbyqxmd.com/read/28730698/boar-sperm-quality-after-supplementation-of-diets-with-omega-3-polyunsaturated-fatty-acids-extracted-from-microalgae
#1
Y T Andriola, F Moreira, E Anastácio, F A Camelo, A C Silva, A S Varela, S M M Gheller, K L Goularte, C D Corcini, T Lucia
This study evaluated effects of diet supplementation with omega-3 polyunsaturated fatty acids (PUFA) from microalgae on boar sperm quality. Two groups of boars (n = 3 each) were fed during 75 days either a commercial diet (control), or the same diet supplemented with omega-3 PUFA from the heterotrophic microalgae Schizochytrium sp. (120 g/kg). Sixteen ejaculates were collected per boar. Some sperm kinetics parameters were inferior for supplemented than for control boars (p < .05): distance average path; distance in both curved and straight line; velocity average path, velocity in both curved and straight line; and amplitude of lateral head displacement...
July 21, 2017: Andrologia
https://www.readbyqxmd.com/read/28728969/a-nanoporous-titanium-surface-promotes-the-maturation-of-focal-adhesions-and-the-formation-of-filopodia-with-distinctive-nanoscale-protrusions-by-osteogenic-cells
#2
Dainelys Guadarrama Bello, Aurélien Fouillen, Antonella Badia, Antonio Nanci
While topography is a key determinant of the cellular response to biomaterials the mechanisms implicated in the cell-surface interactions are complex and still not fully elucidated. In this context, we have examined the effect of nanoscale topography on the formation of filopodia, focal adhesions, and on gene expression of proteins associated with cell adhesion and sensing. Commercially pure titanium discs were treated for oxidative nanopatterning with a solution of H2SO4/H2O2 50:50 (v/v). Scanning electron microscopy and atomic force microscopy characterizations showed that this facile chemical treatment efficiently creates a unique nanoporous surface with a root-mean-square roughness of 11...
July 17, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28720839/effect-of-head-group-and-lipid-tail-oxidation-in-the-cell-membrane-revealed-through-integrated-simulations-and-experiments
#3
M Yusupov, K Wende, S Kupsch, E C Neyts, S Reuter, A Bogaerts
We report on multi-level atomistic simulations for the interaction of reactive oxygen species (ROS) with the head groups of the phospholipid bilayer, and the subsequent effect of head group and lipid tail oxidation on the structural and dynamic properties of the cell membrane. Our simulations are validated by experiments using a cold atmospheric plasma as external ROS source. We found that plasma treatment leads to a slight initial rise in membrane rigidity, followed by a strong and persistent increase in fluidity, indicating a drop in lipid order...
July 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28720385/differences-of-lipid-membrane-modulation-and-oxidative-stress-by-digoxin-and-21-benzylidene-digoxin
#4
Lilian Nd Silva, Silmara Lg Alves, Jessica Venugopal, Vanessa F Cortes, Herica L Santos, José Afp Villar, Leandro A Barbosa
Cardiotonic steroids (CTS) are compounds which bind to the Na,K-ATPase, leading to its inhibition and in some cases initiating signaling cascades. Long utilized as a treatment for congestive heart disease, CTS have more recently been observed to inhibit proliferation and cause apoptosis in several cancer cell lines. A synthetic derivative of the CTS digoxin, called 21-benzylidene digoxin (21-BD), activates the Na,K-ATPase rather than cause its inhibition, as its parent compound does. Here, the mechanism behind the unique effects of 21-BD are further explored...
July 15, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28711161/role-of-fatty-acids-in-cold-adaptation-of-antarctic-psychrophilic-flavobacterium-spp
#5
Stanislava Králová
Cold-loving microorganisms developed numerous adaptation mechanisms allowing them to survive in extremely cold habitats, such as adaptation of the cell membrane. The focus of this study was on the membrane fatty acids of Antarctic Flavobacterium spp., and their adaptation response to cold-stress. Fatty acids and cold-response of Antarctic flavobacteria was also compared to mesophilic and thermophilic members of the genus Flavobacterium. The results showed that the psychrophiles produced more types of major fatty acids than meso- and thermophilic members of this genus, namely C15:1 iso G, C15:0 iso, C15:0 anteiso, C15:1ω6c, C15:0 iso 3OH, C17:1ω6c, C16:0 iso 3OH and C17:0 iso 3OH, summed features 3 (C16:1ω7cand/or C16:1ω6c) and 9 (C16:0 10-methyl and/or C17:1 iso ω9c)...
July 3, 2017: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/28710349/lipid-driven-nanodomains-in-giant-lipid-vesicles-are-fluid-and-disordered
#6
Alena Koukalová, Mariana Amaro, Gokcan Aydogan, Gerhard Gröbner, Philip T F Williamson, Ilya Mikhalyov, Martin Hof, Radek Šachl
It is a fundamental question in cell biology and biophysics whether sphingomyelin (SM)- and cholesterol (Chol)- driven nanodomains exist in living cells and in model membranes. Biophysical studies on model membranes revealed SM and Chol driven micrometer-sized liquid-ordered domains. Although the existence of such microdomains has not been proven for the plasma membrane, such lipid mixtures have been often used as a model system for 'rafts'. On the other hand, recent super resolution and single molecule results indicate that the plasma membrane might organize into nanocompartments...
July 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28710262/med15b-regulates-acid-stress-response-and-tolerance-in-candida-glabrata
#7
Yanli Qi, Hui Liu, Jiayin Yu, Xiulai Chen, Liming Liu
Candida glabrata is a promising producer of organic acids. To elucidate the physiological function of the Mediator tail subunit Med15B in the response to low pH stress, we constructed a deletion strain, med15BΔ, and overexpression strain, HTUΔ/CgMED15B Deletion of MED15B caused biomass production, glucose consumption rate, and cell viability to decrease by 28.3%, 31.7%, and 26.5%, respectively, compared with those of the parent strain (HTUΔ) at pH 2.0. Expression of lipid metabolism-related genes was significantly downregulated in the med15BΔ strain, whereas key genes of ergosterol biosynthesis showed abnormal upregulation...
July 14, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28708394/human-viperin-causes-radical-sam-dependent-elongation-of-escherichia-coli-hinting-at-its-physiological-role
#8
Micah T Nelp, Anthony P Young, Branden M Stepanski, Vahe Bandarian
Viperin (virus inhibitory protein, endoplasmic reticulum-associated, interferon-inducible) is a widely distributed protein that is expressed in response to infection and causes antiviral effects against a broad spectrum of viruses. Viperin is a member of the radical S-adenosyl-l-methionine (SAM) superfamily of enzymes, which typically employ a 4Fe-4S cluster to reductively cleave SAM to initiate chemistry. Though the specific reaction catalyzed by viperin remains unknown, it has been shown that expression of viperin causes an increase in the fluidity of lipid membranes, which impedes the budding of nascent viral particles from the membrane inhibiting propagation of the infection...
July 18, 2017: Biochemistry
https://www.readbyqxmd.com/read/28697671/bacterial-membranes-structure-domains-and-function
#9
Henrik Strahl, Jeff Errington
The bacterial cytoplasmic membrane is composed of roughly equal proportions of lipids and proteins. The main lipid components are phospholipids, which vary in acyl chain length, saturation, and branching and carry head groups that vary in size and charge. Phospholipid variants determine membrane properties such as fluidity and charge that in turn modulate interactions with membrane-associated proteins. We summarize recent advances in understanding bacterial membrane structure and function, focusing particularly on the possible existence and significance of specialized membrane domains...
July 11, 2017: Annual Review of Microbiology
https://www.readbyqxmd.com/read/28689720/thermal-acclimation-in-american-alligators-effects-of-temperature-regime-on-growth-rate-mitochondrial-function-and-membrane-composition
#10
Edwin R Price, Tushar S Sirsat, Sarah K G Sirsat, Gurdeep Kang, Jantana Keereetaweep, Mina Aziz, Kent D Chapman, Edward M Dzialowski
We investigated the ability of juvenile American alligators (Alligator mississippiensis) to acclimate to temperature with respect to growth rate. We hypothesized that alligators would acclimate to cold temperature by increasing the metabolic capacity of skeletal muscles and the heart. Additionally, we hypothesized that lipid membranes in the thigh muscle and liver would respond to low temperature, either to maintain fluidity (via increased unsaturation) or to maintain enzyme reaction rates (via increased docosahexaenoic acid)...
August 2017: Journal of Thermal Biology
https://www.readbyqxmd.com/read/28689316/higher-lipophilic-index-indicates-higher-risk-of-coronary-heart-disease-in-postmenopausal-women
#11
Qing Liu, Alice H Lichtenstein, Nirupa R Matthan, Chanelle J Howe, Matthew A Allison, Barbara V Howard, Lisa W Martin, Carolina Valdiviezo, JoAnn E Manson, Simin Liu, Charles B Eaton
Fatty acids (FAs) are essential components of cell membranes and play an integral role in membrane fluidity. The lipophilic index [LI, defined as the sum of the products between FA levels and melting points (°C), divided by the total amount of FA: [Formula: see text]] is thought to reflect membrane and lipoprotein fluidity and may be associated with the risk of coronary heart disease (CHD). Therefore, we examined the associations of dietary and plasma phospholipid (PL) LI with CHD risk among postmenopausal women...
July 8, 2017: Lipids
https://www.readbyqxmd.com/read/28684313/membrane-fluidity-is-a-driving-force-for-recoverin-myristoyl-immobilization-in-zwitterionic-lipids
#12
Kim Potvin-Fournier, Geneviève Valois-Paillard, Thierry Lefèvre, Line Cantin, Christian Salesse, Michèle Auger
Recoverin is the only protein for which the phenomenon of calcium-myristoyl switch has been demonstrated without ambiguity. It is located in rod disk membranes where the highest content in polyunsaturated lipid acyl chains can be found. However, although essential to better understand the inactivation of the phototransduction process, the role of membrane fluidity on recoverin recruitment is unclear. We have therefore investigated the immobilization of the recoverin myristoyl moiety in the presence of phosphocholine bilayers using (2)H solid-state NMR spectroscopy...
July 3, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28683206/changes-in-the-mitochondrial-properties-may-contribute-to-enhanced-resistance-to-ischemia-reperfusion-injury-in-the-diabetic-rat-heart
#13
Martina Muráriková, Miroslav Ferko, Iveta Waczulíková, Magdaléna Jašová, Ivana Kancirová, Jana Murínová, Táňa Ravingerová
As has been well-documented, diabetes mellitus, besides deleterious effects, induces cardiac adaptation that may reduce its susceptibility to ischemia/reperfusion (I/R) injury. This study aimed to investigate whether changes in mitochondrial properties are involved in the mechanisms of increased resistance of diabetic heart to I/R. Adult male Wistar rats were made diabetic by a single dose of streptozotocin (STZ, 65 mg/kg, i.p.), and on the 8th day, Langendorff-perfused hearts were subjected to 30-min global ischemia/40-min reperfusion...
July 6, 2017: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/28671727/effects-of-global-change-factors-on-fatty-acids-and-mycosporine-like-amino-acid-production-in-chroothece-richteriana-rhodophyta
#14
Daniel Gonzalez-Silvera, Sandra Pérez, Nathalie Korbee, Félix L Figueroa, Antonia D Asencio, Marina Aboal, José Ángel López-Jiménez
Under natural conditions, Chroothece richteriana synthesizes a fairly high proportion of fatty acids. However, nothing is known about how environmental changes affect their production, or about the production of protective compounds, when colonies develop under full sunshine with high levels of UV radiation. In this study wild colonies of C. richteriana were subjected to increasing temperature, conductivity, ammonium concentrations and PAR and UV radiations to assess the potential changes in lipid composition and MAAs concentration...
July 3, 2017: Journal of Phycology
https://www.readbyqxmd.com/read/28670715/lipid-membrane-modulation-and-pigmentation-a-cryoprotection-mechanism-in-arctic-pigmented-bacteria
#15
Archana Singh, Kottekattu P Krishnan, Dharmar Prabaharan, Rupesh K Sinha
The present study aims to address the effect of gradual change in temperature (15-4 °C) followed by freeze-thaw on pigmented bacterial strains - Leeuwenhoekiella aequorea, Pseudomonas pelagia, Halomonas boliviensis, Rhodococcus yunnanensis, and Algoriphagus ratkwoskyi, isolated from Kongsfjorden (an Arctic fjord) to understand their survival in present climate change scenario. The total cell count and retrievability of the isolates were not affected despite the variation in temperature. In all the isolates, the saturated fatty acids, particularly stearic and palmitic acid were predominant at higher temperature, while at 4 °C, the unsaturated fatty acids, primarily cis-10-pentadecenoic, palmitoleic, and oleic acid, were major constituents, confirming homeoviscous adaptation...
July 3, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28668704/effects-of-1-4-naphthoquinone-aged-carbon-black-particles-on-the-cell-membrane-of-human-bronchial-epithelium
#16
Yongming Zhang, Li Wang, Huimin Feng, Guiping Hu, Lele Wang, Jiaxing Liu, Xin Gao, Jing Shang, Tong Zhu, Shichuan Tang, Guang Jia
Black carbon (BC) and polycyclic aromatic hydrocarbons (PAHs), which are major atmospheric pollutants in China, are hazardous to humans following inhalation. BC can be oxidized by PAHs forming secondary particles of which the health effects are unknown. In this study, carbon black (CB) was used to simulate BC to reveal the adverse effects of 1,4-naphthoquinone aged carbon black (CB/1,4-NQ) particles on the membrane of human bronchial epithelial (16HBE) cells. It was showed that, the cell viability, cell membrane fluidity, membrane potential and mitochondrial membrane potential were significantly decreased after 16HBE cells were treated with CB, 1,4-NQ or CB/1,4-NQ...
June 20, 2017: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/28664534/does-triacylglycerol-tag-serve-a-photoprotective-function-in-plant-leaves-an-examination-of-leaf-lipids-under-shading-and-drought
#17
Renée M Marchin, Tarryn L Turnbull, Audrey I Deheinzelin, Mark A Adams
Plant survival in many ecosystems requires tolerance of large radiation loads, unreliable water supply, and suboptimal soil fertility. We hypothesized that increased production of neutral lipids (triacylglycerols, TAGs) in plant leaves is a mechanism for dissipating excess radiation energy. In a greenhouse experiment, we combined drought and shade treatments and examined responses among four species differing in life form, habitat, and drought- and shade-tolerance. We also present a lipid extraction protocol suitable for sclerophyllous leaves of native Australian trees (e...
June 29, 2017: Physiologia Plantarum
https://www.readbyqxmd.com/read/28663766/non-invasive-ventilation-improves-hemorheology-status-in-hypoxemic-patients-with-acute-myocardial-infarction-after-pci
#18
Xi-Fu Wang, Ming Ye, Dong Yan, Hui-Min Zhang, Ping Jia, Xue-Jun Ren, Yu-Jie Zeng
BACKGROUND: Hypoxemia sometimes occurs in the emergency room in the patients with acute myocardial infarction (AMI) after percutaneous coronary intervention (PCI), even in those with administration of conventional high-flow oxygen inhalation. The objective of the present study was to evaluate the effectiveness of non-invasive ventilation (NIV) in improving blood oxygen content and hemorheology in patients with AMI and hypoxemia. METHODS: This prospective study enrolled 50 consecutive eligible patients with AMI (aged 72...
April 2017: Journal of Geriatric Cardiology: JGC
https://www.readbyqxmd.com/read/28660529/inhibition-of-polyunsaturated-fatty-acids-synthesis-decreases-growth-rate-and-membrane-fluidity-of-rhodosporidium-kratochvilovae-at-low-temperature
#19
Jun Wang, Wei Chen, Hongjuan Nian, Xiuling Ji, Lianbing Lin, Yunlin Wei, Qi Zhang
The intention of this study was to investigate the role of polyunsaturated fatty acids (PUFA) in the cold adaptation of Rhodosporidium kratochvilovae YM25235 by knockout of the Δ(12)/Δ(15)-fatty acid desaturase gene (RKD12) to inactivate Δ(12)/Δ(15)-fatty acid desaturase. Polymerase chain reaction (PCR) amplification was used to detect the genomic structure of RKD12 gene in YM25235. The RKD12 gene was knocked out by DNA homologous recombination to inhibit the biosynthesis of PUFA. Then, the contents of linoleic acid (LNA) and α-linolenic acid (ALA) after gene knockout were investigated using a gas chromatography-mass spectrometer, followed by determination of the growth rate and membrane fluidity of YM25235 at low temperature...
June 28, 2017: Lipids
https://www.readbyqxmd.com/read/28658673/hyperthermia-and-associated-changes-in-membrane-fluidity-potentiate-p2x7-activation-to-promote-tumor-cell-death
#20
Paola de Andrade Mello, Shu Bian, Luiz Eduardo Baggio Savio, Haohai Zhang, Jingping Zhang, Wolfgang Junger, Márcia Rosângela Wink, Guido Lenz, Andréia Buffon, Yan Wu, Simon Christopher Robson
Extracellular ATP (eATP) accumulation within the tumor microenvironment (TME) has the potential to activate purinergic signaling. The eATP evoked signaling effects bolster antitumor immune responses while exerting direct cytotoxicity on tumor cells and vascular endothelial cells, mediated at least in part through P2X7 receptors. Approaches to augment purinergic signaling in TME e.g. by ectonucleotidase CD39 blockade, and/or boosting P2X7 functional responses, might be used as immunomodulatory therapies in cancer treatment...
June 21, 2017: Oncotarget
keyword
keyword
111795
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"