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membrane fluidity

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https://www.readbyqxmd.com/read/29909971/phospholipase-pla2g6-a-parkinsonism-associated-gene-affects-vps26-and-vps35-retromer-function-and-ceramide-levels-similar-to-%C3%AE-synuclein-gain
#1
Guang Lin, Pei-Tseng Lee, Kuchuan Chen, Dongxue Mao, Kai Li Tan, Zhongyuan Zuo, Wen-Wen Lin, Liping Wang, Hugo J Bellen
Mutations in PLA2G6 (PARK14) cause neurodegenerative disorders in humans, including autosomal recessive neuroaxonal dystrophy and early-onset parkinsonism. We show that loss of iPLA2-VIA, the fly homolog of PLA2G6, reduces lifespan, impairs synaptic transmission, and causes neurodegeneration. Phospholipases typically hydrolyze glycerol phospholipids, but loss of iPLA2-VIA does not affect the phospholipid composition of brain tissue but rather causes an elevation in ceramides. Reducing ceramides with drugs, including myriocin or desipramine, alleviates lysosomal stress and suppresses neurodegeneration...
June 7, 2018: Cell Metabolism
https://www.readbyqxmd.com/read/29908139/lipidomic-adaptations-of-the-metarhizium-robertsii-strain-in-response-to-the-presence-of-butyltin-compounds
#2
Paulina Stolarek, Sylwia Różalska, Przemysław Bernat
Metarhizium robertsii, a butyltin-resistant filamentous fungus, can rapid and complete biodegradation of di- (DBT) and tributyltin (TBT) under conditions of intensive aeration and ascorbic acid supplementation. In this paper, lipidomic investigations were performed to find the membrane adaptations necessary for effective butyltins degradation. HPLC-MS/MS analysis showed that the phospholipid profile was greatly modified during M. robertsii batch cultivation (pO2  ≥ 20%), contributing to increased membrane fluidity and facilitated mass transfer, which could enhance butyltins biodegradation...
June 13, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29903751/lipophilicity-of-the-cystic-fibrosis-drug-ivacaftor-and-its-destabilizing-effect-on-the-major-cf-causing-mutation-f508del
#3
Stephanie Chin, Maurita Hung, Amy Won, Yu-Sheng Wu, Saumel Ahmadi, Donghe Yang, Salma Elmallah, Krimo Toutah, C Michael Hamilton, Robert N Young, Russell D Viirre, Christopher M Yip, Christine E Bear
The major Cystic Fibrosis (CF) causing mutation, the deletion of phenylalanine at position 508 (F508del) at the cystic fibrosis transmembrane conductance regulator (CFTR), occurs in approximately 90% of the CF population. Recently, a combination therapy, comprising a corrector (VX-809) that rescues the processing defects of F508del-CFTR and a potentiator (VX-770) that rescues mutant channel activity, was approved for CF patients homozygous for this mutation. However, clinical studies revealed that the efficacy of this drug on lung function was modest and variable amongst patients...
June 14, 2018: Molecular Pharmacology
https://www.readbyqxmd.com/read/29903493/a-decision-support-system-for-type-1-diabetes-mellitus-diagnostics-based-on-dual-channel-analysis-of-red-blood-cell-membrane-fluidity
#4
Ermanno Cordelli, Giuseppe Maulucci, Marco De Spirito, Alessandro Rizzi, Dario Pitocco, Paolo Soda
BACKGROUND AND OBJECTIVE: Investigation of membrane fluidity by metabolic functional imaging opens up a new and important area of translational research in type 1 diabetes mellitus, being a useful and sensitive biomarker for disease monitoring and treatment. We investigate here how data on membrane fluidity can be used for diabetes monitoring. METHODS: We present a decision support system that distinguishes between healthy subjects, type 1 diabetes mellitus patients, and type 1 diabetes mellitus patients with complications...
August 2018: Computer Methods and Programs in Biomedicine
https://www.readbyqxmd.com/read/29902419/bactericidal-activity-of-amphipathic-cationic-antimicrobial-peptides-involves-altering-the-membrane-fluidity-when-interacting-with-the-phospholipid-bilayer
#5
Soraya Omardien, Jan W Drijfhout, Frédéric M Vaz, Michaela Wenzel, Leendert W Hamoen, Sebastian A J Zaat, Stanley Brul
BACKGROUND: Amphipathic cationic antimicrobial peptides (AMPs) TC19 and TC84, derived from the major AMPs of human blood platelets, thrombocidins, and Bactericidal Peptide 2 (BP2), a synthetic designer peptide showed to perturb the membrane of Bacillus subtilis. We aimed to determine the means by which the three AMPs cause membrane perturbation in vivo using B. subtilis and to evaluate whether the membrane alterations are dependent on the phospholipid composition of the membrane. METHODS: Physiological analysis was employed using Alexa Fluor 488 labelled TC84, various fluorescence dyes, fluorescent microscopy techniques and structured illumination microscopy...
June 11, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29900630/n-terminal-dual-lipidation-coupled-molecular-targeting-into-the-primary-cilium
#6
Masahiro Kumeta, Yulia Panina, Hiroya Yamazaki, Kunio Takeyasu, Shige H Yoshimura
The primary cilium functions as an "antenna" for cell signaling, studded with characteristic transmembrane receptors and soluble protein factors, raised above the cell surface. In contrast to the transmembrane proteins, targeting mechanisms of nontransmembrane ciliary proteins are poorly understood. We focused on a pathogenic mutation that abolishes ciliary localization of retinitis pigmentosa 2 protein and revealed a dual acylation-dependent ciliary targeting pathway. Short N-terminal sequences which contain myristoylation and palmitoylation sites are sufficient to target a marker protein into the cilium in a palmitoylation-dependent manner...
June 13, 2018: Genes to Cells: Devoted to Molecular & Cellular Mechanisms
https://www.readbyqxmd.com/read/29893945/characterization-of-storage-induced-red-blood-cell-hemolysis-using-raman-spectroscopy
#7
Rekha Gautam, Joo-Yeun Oh, Marisa B Marques, Richard A Dluhy, Rakesh P Patel
Background: The therapeutic efficacy and safety of stored red blood cells (RBCs) relies on minimal in-bag hemolysis. The accuracy of current methods of measuring hemolysis can suffer as a result of specimen collection and processing artefacts. Objective: To test whether Raman spectroscopy could be used to assess hemolysis. Methods: RBCs were stored for as long as 42 days. Raman spectra of RBCs were measured before and after washing, and hemolysis was measured in supernatant by visible spectroscopy...
June 11, 2018: Laboratory Medicine
https://www.readbyqxmd.com/read/29885356/laurdan-fluorescence-and-phasor-plots-reveal-the-effects-of-a-h-2-o-2-bolus-in-nih-3t3-fibroblast-membranes-dynamics-and-hydration
#8
REVIEW
Leonel Malacrida, Enrico Gratton
Fluorescence spectroscopy, coupled with microscopy, opens new frontiers for the study of dynamic processes with high spatio-temporal resolution. The application of phasor plots to FLIM and hyperspectral imaging demonstrate unprecedented capabilities to study complex photophysics at the subcellular level. Using these approaches we studied the effects of an H2 O2 bolus on NIH-3T3 membranes dynamics monitored by LAURDAN fluorescence. Exposure of NIH-3T3 cells to a bolus of H2 O2 modifies the cell membranes and, in particular, the plasma membrane in a complex manner...
June 6, 2018: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/29864488/changes-in-the-lipid-composition-of-bradyrhizobium-cell-envelope-reveal-a-rapid-response-to-water-deficit-involving-lysophosphatidylethanolamine-synthesis-from-phosphatidylethanolamine-in-outer-membrane
#9
Adriana B Cesari, Natalia S Paulucci, María A Biasutti, Gustavo M Morales, Marta S Dardanelli
We evaluate the behavior of the membrane of Bradyrhizobium sp. SEMIA6144 during adaptation to polyethylene glycol (PEG). A dehydrating effect on the morphology of the cell surface, as well as a fluidizing effect on the membrane was observed 10 min after PEG shock; however, the bacteria were able to restore optimal membrane fluidity. Shock for 1 h caused an increase of lysophosphatidylethanolamine in the outer membrane at the expense of phosphatidylcholine and phosphatidylethanolamine, through an increase in phospholipase activity...
June 1, 2018: Research in Microbiology
https://www.readbyqxmd.com/read/29864405/studies-on-the-interactions-of-anticancer-drug-minerval-with-membrane-lipids-in-binary-and-ternary-langmuir-monolayers
#10
Karolina Węder, Marzena Mach, Katarzyna Hąc-Wydro, Paweł Wydro
2-Hydroxyoleic acid (2OHOA, Minerval), a derivative of oleic acid, is the lipid used in Membrane Lipid Therapy. This compound is of confirmed anticancer effect, however its exact mechanism of action has not been fully elucidated. In this work the interactions of 2OHOA with cholesterol, sphingomyelin and phosphatidylcholine in Langmuir films were investigated. Moreover, the influence of this drug on SM/Chol and POPC/Chol films was studied. The collected results evidenced that 2OHOA substantially increases fluidity of lipid monolayers and modifies membrane organization, however, its influence depends on drug concentration and membrane properties...
June 1, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29849946/pivotal-role-of-human-stearoyl-coa-desaturases-scd1-and-5-in-breast-cancer-progression-oleic-acid-based-effect-of-scd1-on-cell-migration-and-a-novel-pro-cell-survival-role-for-scd5
#11
Cristiana Angelucci, Alessio D'Alessio, Fortunata Iacopino, Gabriella Proietti, Alba Di Leone, Riccardo Masetti, Gigliola Sica
The influence of cell membrane fluidity on cancer progression has been established in different solid tumors. We previously reported that "cancer-associated fibroblasts" (CAFs) induced epithelial-mesenchymal transition and increased cell membrane fluidity and migration in poorly (MCF-7) and highly invasive (MDA-MB-231) breast cancer cells. We also found that the membrane fluidity regulating enzyme stearoyl-CoA desaturase 1 (SCD1) was upregulated in tumor cells co-cultured with CAFs and established its essential role for both intrinsic and CAF-driven tumor cell motility...
May 11, 2018: Oncotarget
https://www.readbyqxmd.com/read/29806994/interaction-of-gabaergic-ketones-with-model-membranes-a-molecular-dynamics-and-experimental-approach
#12
Virginia Miguel, Mariela E Sánchez-Borzone, Daniel A García
γ-Aminobutyric-acid receptor (GABAA -R), a membrane intrinsic protein, is activated by GABA and modulated by a wide variety of recognized drugs. GABAA -R is also target for several insecticides which act by recognition of a non-competitive blocking site. Mentha oil is rich in several ketones with established activity against various insects/pests. Considering that mint ketones are highly lipophilic, their action mechanism could involve, at least in part, a non-specific receptor modulation by interacting with the surrounding lipids...
May 25, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29803841/the-second-generation-tyrosine-kinase-inhibitor-dasatinib-induced-eryptosis-in-human-erythrocytes-an-in-vitro-study
#13
Wai Yin Chan, Pui Man Lau, Ka Wing Yeung, Siu Kai Kong
Dasatinib, a new tyrosine kinase inhibitor, is used clinically to kill chronic myelogenous leukemia and acute lymphoblastic leukemia through apoptosis. Obviously, anemia is developed in many patients receiving dasatinib for treatment. Until now, the mechanism for the cytotoxic effects of dasatinib in human erythrocytes is not fully understood. As many tyrosine kinases are found in human erythrocytes, it is therefore logical to hypothesize that dasatinib is able to induce apoptosis (or eryptosis) in human erythrocytes...
May 24, 2018: Toxicology Letters
https://www.readbyqxmd.com/read/29799188/nanoscale-investigation-into-the-cellular-response-of-glioblastoma-cells-exposed-to-protons
#14
Ewelina Lipiec, Bayden R Wood, Andrzej Kulik, Wojciech M Kwiatek, Giovanni Dietler
Exposure to ionizing radiation can induce cellular defense mechanisms including cell activation and rapid proliferation prior to metastasis and in extreme cases can result in cell death. Herewith we apply infrared nano- and microspectroscopy combined with multidimensional data analysis to characterize the effect of ionizing radiation on single glioblastoma nuclei isolated from cells treated with 10 Gy of X-rays or 1 and 10 Gy of protons. We observed chromatin fragmentation related to the formation of apoptotic bodies following X-ray exposure...
June 6, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29788718/morphology-and-physical-properties-of-hydrophilic-polymer-modified-lipids-in-supported-lipid-bilayers
#15
Yasuhiro Kakimoto, Yoshihiro Tachihara, Yoshiaki Okamoto, Keisuke Miyazawa, Takeshi Fukuma, Ryugo Tero
Lipid molecules such as glycolipids which are modified with hydrophilic biopolymers participate in the biochemical reactions occurring on cell membranes. Their functions and efficiency are determined by the formation of microdomains and their physical properties. We investigated the morphology and properties of domains induced by the hydrophilic-polymer-modified lipid applying poly-ethylene glycol (PEG)-modified lipid as a model modified lipid. We formed supported lipid bilayers (SLBs) using a 0-10 mol % range of PEG-modified lipid concentration (CPEG)...
May 23, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29786073/integrin-%C3%AE-6%C3%AE-4-src-akt-signaling-induces-cellular-senescence-by-counteracting-apoptosis-in-irradiated-tumor-cells-and-tissues
#16
Seung Hee Jung, Minyoung Lee, Hyun A Park, Hyung Chul Lee, Donghee Kang, Hyun Jung Hwang, Chanho Park, Dong-Min Yu, Yu Ri Jung, Mi-Na Hong, Yong-Nyun Kim, Heon Joo Park, Young-Gyu Ko, Jae-Seon Lee
Cellular senescence refers to an irreversible growth arrest that is triggered by various intrinsic and extrinsic stresses. Many recent studies have demonstrated that cellular senescence plays a crucial role in the regression of tumors exposed to ionizing radiation (IR), but the underlying mechanism remains unknown. Here we show that the activation of integrin β4 is essential for IR-induced cellular senescence. IR treatment results in the phosphorylation of integrin β4 at tyrosine residue 1510, leading to activation of the integrin α6β4-Src-AKT signaling pathway...
May 21, 2018: Cell Death and Differentiation
https://www.readbyqxmd.com/read/29782965/membrane-cholesterol-delays-cellular-apoptosis-induced-by-ginsenoside-rh2-a-steroid-saponin
#17
Sandrine L Verstraeten, Marie Albert, Adrien Paquot, Giulio G Muccioli, Donatienne Tyteca, Marie-Paule Mingeot-Leclercq
Saponins exhibit several biological and pharmacological activities, such as antibacterial, anti-inflammatory and anticancer effects. Many studies attribute their activities to their interactions with cholesterol. In this study, we focus on the steroid saponin ginsenoside Rh2, one of the active principles of Panax ginseng root. Some evidence suggests that lipid rafts, defined as nanodomains enriched in cholesterol and sphingolipids, could be involved in the Rh2-induced apoptosis. However, the role of membrane lipids, especially cholesterol, in this process is still poorly understood...
May 18, 2018: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29782818/antimicrobial-activity-profiles-of-amphiphilic-xanthone-derivatives-are-a-function-of-their-molecular-oligomerization
#18
Jun-Jie Koh, Shuimu Lin, Yang Bai, Wendy Wan Ling Sin, Thet Tun Aung, Jianguo Li, Verma Chandra, Konstantin Pervushin, Roger W Beuerman, Shouping Liu
Currently, membrane-targeting small antimicrobial peptidomimetics (SAP) are important in antibiotic development because bacteria appear to develop resistance to these surface-active compounds less readily. However, the molecular membrane-targeting action of SAPs has received little attention. In this study, we investigated the effect of oligomerization of amphiphilic xanthone, a model SAP, on its antimicrobial properties against both Gram-positive and Gram-negative bacteria. First, oligomer formation by an amphiphilic xanthone, compound 2 (also coded as AM052), was investigated via solution-state nuclear magnetic resonance (NMR) spectroscopy...
May 18, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29778781/fullerenol-c-60-oh-36-protects-human-erythrocyte-membrane-against-high-energy-electrons
#19
Jacek Grebowski, Paulina Kazmierska, Grzegorz Litwinienko, Anna Lankoff, Marian Wolszczak, Anita Krokosz
Fullerenols (polyhydroxylated fullerene C60 ) are nanomaterial with potentially broad applicability in biomedical sciences with high antioxidant ability, thus, we investigated the radioprotecting potential of fullerenol C60 (OH)36 on human erythrocytes irradiated by high-energy electrons of 6 MeV. The results demonstrate that C60 (OH)36 at concentration of 150 μg/mL protects the erythrocytes against the radiation-induced hemolysis (comparing to non-protected cells, we observed 30% and 39% protection for 0...
May 17, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29776647/-effect-of-green-alga-ulva-lactuca-polysaccharides-supplementation-on-blood-pressure-and-on-atherogenic-risk-factors-in-rats-fed-a-high-fat-diet
#20
Z I Tair, F Bensalah, F Boukortt
AIM OF THE STUDY: To highlight the benefits of green alga Ulva lactuca polysaccharides supplementation on blood pressure and atherogenic risk factors in rats fed a high fat diet. METHODS: Wistar male rats were fed a high fat diet (30% sheep fat) for 3 months. At an average body weight (BW) of 360g, the rats (n=18) were divided into 3 groups and consumed, for 28 days, either a high fat diet (HFD) or a high fat diet enriched with 1% of whole green algae (WGA) powder or with 1% of its polysaccharides (PLS)...
May 15, 2018: Annales de Cardiologie et D'angéiologie
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