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https://www.readbyqxmd.com/read/29161329/comparative-metabolomics-profiling-of-engineered-saccharomyces-cerevisiae-lead-to-a-strategy-that-improving-%C3%AE-carotene-production-by-acetate-supplementation
#1
Xiao Bu, Liang Sun, Fei Shang, Guoliang Yan
A comparative metabolomic analysis was conducted on recombinant Saccharomyces cerevisiae strain producing β-carotene and the parent strain cultivated with glucose as carbon source using gas chromatography-mass spectrometry (GC-MS), high performance liquid chromatography-mass spectrometry (HPLC-MS) and ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) based approach. The results showed that most of the central intermediates associated with amino acids, carbohydrates, glycolysis and TCA cycle intermediates (acetic acid, glycerol, citric acid, pyruvic acid and succinic acid), fatty acids, ergosterol and energy metabolites were produced in a lower amount in recombinant strain, as compared to the parent strain...
2017: PloS One
https://www.readbyqxmd.com/read/29161327/thallium-stimulates-ethanol-production-in-immortalized-hippocampal-neurons
#2
Laura Colombaioni, Massimo Onor, Edoardo Benedetti, Emilia Bramanti
Lactate and ethanol (EtOH) were determined in cell culture medium (CCM) of immortalized hippocampal neurons (HN9.10e cell line) before and after incubation with Thallium (Tl). This cell line is a reliable, in vitro model of one of the most vulnerable regions of central nervous system. Cells were incubated for 48 h with three different single Tl doses: 1, 10, 100 μg/L (corresponding to 4.9, 49 and 490 nM, respectively). After 48 h, neurons were "reperfused" with fresh CCM every 24/48 h until 7 days after the treatment and the removed CCM was collected and analysed...
2017: PloS One
https://www.readbyqxmd.com/read/29161030/nanoplastic-in-the-north-atlantic-subtropical-gyre
#3
Alexandra Ter Halle, Laurent Jeanneau, Marion Martignac, Emilie Jardé, Boris Pedrono, Laurent Brach, Julien Gigault
Plastics can be found in all ecosystems across the globe. This type of environmental pollution is important, even if its impact is not fully understood. The presence of small plastic particles at the micro- and nanoscales is of growing concern, but nanoplastic has not yet been observed in natural samples. In this study, we examined four size fractions (meso-, large micro-, small micro-, and nanoplastics) of debris collected in the North Atlantic subtropical gyre. To obtain the nanoplastic portion, we isolated the colloidal fraction of seawater...
November 21, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/29160848/application-of-enzymatic-extracts-from-a-calb-standard-strain-as-biocatalyst-within-the-context-of-conventional-biodiesel-production-optimization
#4
Carlos Luna, Diego Luna, Felipa M Bautista, Rafael Estevez, Juan Calero, Alejandro Posadillo, Antonio A Romero, Enrique D Sancho
The application of biocatalysts in the transesterification process of triglycerides (TG) allows integrating the glycerol in the form of monoglyceride (MG), sharply increasing the yield and the environmental sustainability of the conventional biodiesel production process. This is known as Ecodiesel. To overcome the inconvenient of the high cost of the currently employed highly purified commercial enzymes, the use of scarcely purified extracts obtained from standard strains of the same species of commercial lipases currently applied in this process is being investigated...
November 21, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29160760/identification-of-organic-compounds-in-landfill-leachate-treated-by-advanced-oxidation-processes
#5
Ana Paula Jambers Scandelai, Eliane Sloboda Rigobello, Beatriz Lopes Corso de Oliveira, Célia Regina Granhen Tavares
Landfill leachates are considered to be complex effluents of a variable composition containing many compounds which are difficult to biodegrade and is highly toxic both to the environment and to human health.For this purpose and considering the shortage of studies concerning the treatment of landfill leachates using ozone, as well as its combination with catalysts, the aim of this paper was to identify the organic compounds in the landfill leachate of Maringá, Paraná, Brazil treated with advanced oxidation processes (AOPs) of ozonation (O3), heterogeneous catalytic ozonation with TiO2 (O3/TiO2), and heterogeneous catalytic ozonation with ZnO (O3/ZnO)...
November 21, 2017: Environmental Technology
https://www.readbyqxmd.com/read/29160327/imbalance-in-amino-acid-and-purine-metabolisms-at-the-hypothalamus-in-inflammation-associated-depression-by-gc-ms
#6
Yu Wu, Yonghong Li, Yanjuan Jia, Chaojun Wei, Hui Xu, Rui Guo, Yuanting Li, Jing Jia, Xiaoming Qi, Xiaoling Gao
Hypothalamic dysfunction is a key factor in depression; increasing evidence highlights neuroinflammation abnormalities as well as imbalances in neurotransmitters and the purinergic system in the pathophysiology of depression. However, little is known about the metabolomic changes in the hypothalamus of depressed patients with neuroinflammation. Herein, taking advantage of the well-established lipopolysaccharide (LPS)-induced depression mouse model, we measured metabolic changes in the hypothalamus using gas chromatography-mass spectrometry (GC-MS)...
November 21, 2017: Molecular BioSystems
https://www.readbyqxmd.com/read/29159892/reliability-of-body-weight-scalars-on-the-assessment-of-propofol-induction-dose-in-obese-patients
#7
A M Araújo, H S Machado, A C Falcão, P Soares-da-Silva
BACKGROUND: Obese patients require specific perioperative care when compared with non-obese patients. The present study aimed to analyse the ability of size descriptors to estimate propofol induction dose in class II and III obese patients. METHODS: A cross-sectional study on adult patients with body mass index (BMI) equal to or greater than 35 kg/m(2) and on adult patients with BMI lower than 35 kg/m(2) was carried out. General anaesthesia was induced with remifentanil, propofol and rocuronium...
November 21, 2017: Acta Anaesthesiologica Scandinavica
https://www.readbyqxmd.com/read/29159689/antibacterial-and-antioxidant-metabolites-of-diaporthe-spp-isolated-from-flowers-of-melodorum-fruticosum
#8
Chutima Tanapichatsakul, Sakon Monggoot, Eleni Gentekaki, Patcharee Pripdeevech
Fifty-two strains of endophytic fungi were isolated from flowers of the medicinal plant Melodorum fruticosum. Seven genera were identified including Alternaria, Aspergillus, Colletotrichum, Diaporthe, Fusarium, Greeneria and Nigrospora. All strains were cultured for 30 days and further macerated in ethyl acetate solvent for 3 days. The obtained fungal extracts were examined for antibacterial activity using agar disc diffusion against nine pathogenic bacteria: Staphylococcus aureus, Bacillus subtilis, B. cereus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Escherichia coli, Shigella flexneri, Vibrio cholerae and V...
November 20, 2017: Current Microbiology
https://www.readbyqxmd.com/read/29159438/polybrominated-diphenyl-ethers-pbdes-and-hydroxylated-pbdes-in-human-serum-from-shanghai-china-a-study-on-their-presence-and-correlations
#9
Bentuo Xu, Minghong Wu, Mingnan Wang, Chenyuan Pan, Wenhui Qiu, Liang Tang, Gang Xu
Polybrominated diphenyl ethers (PBDEs) are becoming a public health concern because of their potential toxicity, from endocrine disruption system to neurodevelopmental impairments. Nonetheless, information on their levels in human blood is scarce. In this study, human serum samples collected in Shanghai, China, were analyzed for the concentrations of PBDEs and their hydroxylated metabolites (OH-PBDEs). Eight PBDE congeners and six OH-PBDE congeners were quantified in serum samples by gas chromatography with mass spectrometry (GC-MS) and high-performance liquid chromatography with tandem mass spectrometry (HPLC-MS/MS)...
November 20, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29158770/does-the-enterolactone-enl-affect-fatty-acid-transporters-and-lipid-metabolism-in-liver
#10
Krzysztof Drygalski, Klaudia Berk, Tomasz Charytoniuk, Nicoletta Iłowska, Bartłomiej Łukaszuk, Adrian Chabowski, Karolina Konstantynowicz-Nowicka
Background: NAFLD as a result of inappropriate diet and obesity, may progress to sever conditions such as: type 2 diabetes mellitus or steatohepatitis, and has recently become a prevalent topic of numerous investigations. Due to its dangerous aftermaths, finding new substances, such as polyphenols and their derivatives, which might reduce liver steatosis is the main target of research into NAFLD treatment. Hence, the aim of the present study was to evaluate the effect(s) of enterolactone (ENL), a metabolite of secoisolariciresinol (SECO), on lipid metabolism together with changes in the expression of fatty acid transporters in fatty liver...
2017: Nutrition & Metabolism
https://www.readbyqxmd.com/read/29158542/metabolic-profiling-of-umbilical-cord-blood-in-macrosomia
#11
H Sun, Y Wang, C Wang, X Xu, Y Wang, H Yan, X Yang
BACKGROUND/OBJECTIVE: The term macrosomia is used to describe neonates with a birth weight of 4000 g or more. Macrosomia is a potential risk factor for obesity and metabolic syndromes in postnatal and adult life, yet little is known about its associations with metabolic difference in the early-age. We performed metabolic profiling of umbilical cord blood to discover differential metabolites of macrosomia. METHODS: We conducted a case-control study of full-term singletons with normal maternal glucose tolerance [50 cases (macrosomia, birth weight ⩾4000 g); 50 controls (normal weight, birth weight 2500-3999 g)]...
November 21, 2017: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/29157001/screening-of-analgesic-activity-of-tunisian-urtica-dioica-and-analysis-of-its-major-bioactive-compounds-by-gcms
#12
Raouia Dhouibi, Dorsaf Moalla, Kamilia Ksouda, Maryem Ben Salem, Serria Hammami, Zouheir Sahnoun, Khaled Mounir Zeghal, Hanen Affes
The present study was aimed to evaluate the analgesic properties of Urtica dioica (UD) and to profile phytochemicals by gas chromatography-mass spectrometry (GC-MS). The ethanolic extracts were prepared by maceration method and extraction using rotary evaporator. The analgesic activity was analysed by hot plate method, formalin test, acetic acid-induced writhing test and the tail-flick test with different doses of the ethanolic extract. In all tests, the leaf's ethanolic extract exhibited significant analgesic activity (p < ...
November 20, 2017: Archives of Physiology and Biochemistry
https://www.readbyqxmd.com/read/29156967/seed-metabolite-profiling-of-vicia-species-from-china-via-gc-ms
#13
Ann Abozeid, Jia Liu, Yanming Ma, Yang Liu, Xiaorui Guo, Zhonghua Tang
In this study, we examined Vicia seeds using gas chromatography-mass spectrometry (GC-MS). The metabolic differences of seeds of twelve Vicia species were assessed. 184 metabolites were identified. Vicia species were classified via multivariate data analyses into four clusters. V. unijuga was most enriched in fatty acids and anthraquinones contents while highest levels of amino acids, alcohols and phenolic were in V. costata. Clustering analysis of biochemical profiles matched with the pervious phenotypic observation with all examined species from section Cracca grouped together under one sub-cluster, except for V...
November 20, 2017: Natural Product Research
https://www.readbyqxmd.com/read/29156274/determination-of-2-6-di-tert-butyl-hydroxytoluene-and-its-transformation-products-in-indoor-dust-and-sediment-by-gas-chromatography-mass-spectrometry-coupled-with-precolumn-derivatization
#14
Ruirui Zhang, Chao Li, Yunzi Li, Xinyi Cui, Lena Q Ma
We developed an analytical method to simultaneously determine 2,6-di-tert-butyl-hydroxytoluene (BHT) and its four transformation products in indoor dust and sediment samples. BHT, 2,6-di-tert-butylcyclohexa-2,5-diene-1,4-dione (BHT-Q), and 3,5-di-tert-butyl-4-hydroxybenzaldehyde (BHT-CHO) were measured by gas chromatography-mass spectrometry (GC-MS) after ultrasonic extraction with hexane/dichloromethane (1:3), while 2,6-di-tert-butyl-4-(hydroxymethyl) phenol (BHT-OH) and 3,5-di-tert-butyl-4-hydroxybenzoic acid (BHT-COOH) were derivatized using N, O-bis (trimethylsilyl) trifluoroacetamide before GC-MS analysis...
November 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29156272/a-quantitative-assessment-of-distributions-and-sources-of-tropospheric-halocarbons-measured-in-singapore
#15
Sayantan Sarkar, Wei Hong Fan, Shiguo Jia, Donald R Blake, Jeffrey S Reid, Puji Lestari, Liya E Yu
This work reports the first ground-based atmospheric measurements of 26 halocarbons in Singapore, an urban-industrial city-state in Southeast (SE) Asia. A total of 166 whole air canister samples collected during two intensive 7 Southeast Asian Studies (7SEAS) campaigns (August-October 2011 and 2012) were analyzed for C1-C2 halocarbons using gas chromatography-electron capture/mass spectrometric detection. The halocarbon dataset was supplemented with measurements of selected non-methane hydrocarbons (NMHCs), C1-C5 alkyl nitrates, sulfur gases and carbon monoxide to better understand sources and atmospheric processes...
November 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29154880/consolidated-bioprocessing-for-production-of-polyhydroxyalkanotes-from-red-algae-gelidium-amanssi
#16
Shailesh S Sawant, Bipinchandra K Salunke, Beom Soo Kim
Noncompetitive carbon sources such as algae are unconventional and promising raw material for sustainable biofuel production. The capability of one marine bacterium, Saccharophagus degradans 2-40 to degrade red seaweed Gelidium amansii for production of polyhydroxyalkanoates (PHA) was evaluated in this study. S. degradans can readily attach to algae, degrade algal carbohydrates, and utilize that material as main carbon source. Minimal media containing 8g/L G. amansii were used for the growth of S. degradans...
November 14, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29154671/express-forensic-drug-identification-confirmation-and-quantification-using-a-fully-integrated-gas-chromatography-fourier-transform-infrared-mass-spectrometer-gc-ft-ir-ms
#17
Adam Lanzarotta, Lisa Lorenz, Sarah Voelker, Travis M Falconer, JaCinta Batson
No abstract text is available yet for this article.
January 1, 2017: Applied Spectroscopy
https://www.readbyqxmd.com/read/29154397/effects-of-high-ambient-temperature-on-plasma-metabolomic-profiles-in-chicks
#18
Shozo Tomonaga, Hirofumi Okuyama, Tetsuya Tachibana, Ryosuke Makino
Exposure to high ambient temperature is detrimental to the poultry industry. To understand the influence from a metabolic perspective, we investigated the effects of exposure to high ambient temperature on plasma low-molecular-weight metabolite levels in chicks using gas chromatography/mass spectrometry-based non-targeted metabolomic analysis. Heat exposure for 4 days suppressed growth and food intake. Of the 92 metabolites identified, the levels of 29 decreased, whereas the levels of nine increased. We performed an enrichment analysis on the identified metabolites and found 35 candidates for metabolic processes affected by heat exposure...
November 20, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/29154179/the-effect-of-follicullar-fluid-pesticides-and-polychlorinated-biphenyls-concentrations-on-intracytoplasmic-sperm-injection-icsi-embryological-and-clinical-outcome
#19
Tarek K Al-Hussaini, Ahmed A Abdelaleem, Ihab Elnashar, Omar M Shabaan, Rashad Mostafa, Mona A H El-Baz, Sahar E M El-Deek, Tarek A Farghaly
OBJECTIVES: The present study evaluated the correlation between the concentrations of polychlorinated biphenyls (PCBs) and pesticides in the follicular fluid (FF) obtained during intracytoplasmic sperm injection (ICSI) with the ovarian response, endometrial thickness, and embryological and clinical outcomes. DESIGN: Cross-sectional observational study. MATERIALS AND METHODS: Women aged 20 to 38 years (300 infertile couples) presenting to a university-affiliated fertility center were approached to participate in the study...
November 15, 2017: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/29154120/optimization-of-multi-residue-method-for-targeted-screening-and-quantitation-of-243-pesticide-residues-in-cardamom-elettaria-cardamomum-by-gas-chromatography-tandem-mass-spectrometry-gc-ms-ms-analysis
#20
T P Ahammed Shabeer, Rushali Girame, Sagar Utture, Dasharath Oulkar, Kaushik Banerjee, D Ajay, Ranjith Arimboor, K R K Menon
Higher matrix interference makes the multi-residue pesticide analysis in spices more challenging. A simple, sensitive, and robust large-scale multi-residue method was developed for the rapid analysis of 243 pesticides in cardamom matrix by gas chromatography tandem mass spectrometry (GC-MS/MS). Prehydration of cardamom in 1:4 sample:water for 30 min improved the homogeneity and extractability. QuEChERS extraction followed by cleanup with 25 mg primary secondary amine, 100 mg C18, and 10 mg graphitized carbon black to 1 ml supernatant was used for sample preparation...
November 1, 2017: Chemosphere
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