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https://www.readbyqxmd.com/read/28109919/the-ozone-like-syndrome-in-durum-wheat-triticum-durum-desf-mechanisms-underlying-the-different-symptomatic-responses-of-two-sensitive-cultivars
#1
Valentina Picchi, Robert Monga, Riccardo Marzuoli, Giacomo Gerosa, Franco Faoro
Colombo and Sculptur are two modern durum wheat cultivars that, in previous studies, proved to be very sensitive to ozone injury in terms of eco-physiological parameters and significant grain yield loss. Nevertheless, their response regarding leaf visible symptoms was very different; Sculptur showed almost no symptoms, even after several weeks of ozone exposure, whereas Colombo showed in a few weeks typical ozone-like symptoms (chlorotic/necrotic spots). The mechanisms underlying this different response has been studied with a biochemical and microscopical approach...
January 12, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28109808/selective-adsorption-of-pb-ii-ions-by-amylopectin-g-poly-acrylamide-co-acrylic-acid-a-bio-degradable-graft-copolymer
#2
Dinabandhu Sasmal, Jayanta Maity, Haradhan Kolya, Tridib Tripathy
Amylopectin-g-poly (acrylamide-co-acrylic acid) [AP-g-poly (AM-co-AA)] was synthesised in water medium by using potassium perdisulphate as an initiator. The graft copolymer was characterized by molecular weight determination by size exclusion chromatography (SEC), fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance (NMR) spectroscopy, scanning electron microscope (SEM) studies, thermal analysis, measurement of neutralisation equivalent and biodegradation studies. The graft copolymer was used for Pb (II) ion removal from aqueous solution...
January 18, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28109651/late-onset-krabbe-disease-due-to-the-new-galc-p-ala543pro-mutation-with-intriguingly-high-residual-galc-activity-in%C3%A2-vitro
#3
Inge Krägeloh-Mann, Klaus Harzer, Kevin Rostásy, Stefanie Beck-Wödl, Antje Bornemann, Judith Böhringer, Andrea Bevot, Verena Beck, Gisela Merkel, Maria Pechan
BACKGROUND: Krabbe disease (KD) is an inherited leukodystrophy due to a defect in the GALC gene which encodes the lysosomal galactosylceramide β-galactosidase (GALC). About two thirds of patients show the early onset form of KD dominated by cerebral demyelination leading to death in early infancy. Late onset forms include a spectrum of late infantile, juvenile and adult clinical courses. The deficiency of GALC leads to a galactosylceramide lipidosis in which lysosomal storage phenomena are seen almost only at the ultrastructural level...
January 6, 2017: European Journal of Paediatric Neurology: EJPN
https://www.readbyqxmd.com/read/28109291/systemic-nocardiosis-in-a-dog-caused-by-nocardia-cyriacigeorgica
#4
Yesari Eroksuz, Nafia Canan Gursoy, Tolga Karapinar, Burak Karabulut, Canan Akdeniz Incili, Zeynep Yerlikaya, Zulal Asci Toraman, Mehmet Ozkan Timurkan, Hatice Eroksuz
BACKGROUND: Systemic nocardiosis due to Nocardia cyriacigeorgica has not been reported in dogs. CASE PRESENTATION: Light and electron microscopy, microbiological culture and molecular identification (PCR) were used to diagnose systemic nocardiosis caused by Nocardia cyriacigeorgica in a 3-month-old husky dog. The postmortem changes included multifocal to coalescing, sharply circumscribed pyogranulomatous inflammation and abscess formation in lungs, liver, myocardium, spleen, kidneys, brain, and hilar lymph nodes...
January 21, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28109140/-transplantation-of-bone-marrow-mesenchymal-stem-cell-improves-antioxidant-capacity-and-immune-activity-of-aging-model-rats
#5
Zhihong Wang, Yun Lin, Weimin Chen, Jin Shang, Tiannan Wei
Objective To investigate the impact of bone marrow mesenchymal stem cell (BMSC) transplantation on the antioxidant capacity and immune activity of aging rats induced by D-galactose. Methods Ten healthy male SD rats served as a control group (aged 2 months). To establish aging models, healthy SD rats were daily injected subcutaneously with D-galactose (400 mg/kg). Then the aging model rats were randomized into aging model group and BMSC group (ten rats in each group). The BMSC group was injected with 3×10(6) BMSCs via tail vein...
February 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28108399/fine-structure-of-the-anterior-median-eyes-of-the-funnel-web-spider-agelena-labyrinthica-araneae-agelenidae
#6
Wolfgang-D Schröer
Only few electron microscopic studies exist on the structure of the main eyes (anterior median eyes, AME) of web spiders. The present paper provides details on the anatomy of the AME in the funnel-web spider Agelena labyrinthica. The retina consists of two separate regions with differently arranged photoreceptor cells. Its central part has sensory cells with rhabdomeres on 2, 3, or 4 sides, whereas those of the ventral retina have only two rhabdomeres on opposite sides. In addition, the rhabdomeres of the ventral retina are arranged in a specific way: Whereas in the most ventral part they form long tangential rows, those towards the center are detached and are arranged radially...
January 17, 2017: Arthropod Structure & Development
https://www.readbyqxmd.com/read/28108366/functional-analysis-of-a-tyrosinase-gene-involved-in-early-larval-shell-biogenesis-in-crassostrea-angulata-and-its-response-to-ocean-acidification
#7
Bingye Yang, Fei Pu, Lingling Li, Weiwei You, Caihuan Ke, Danqing Feng
The formation of the primary shell is a vital process in marine bivalves. Ocean acidification largely influences shell formation. It has been reported that enzymes involved in phenol oxidation, such as tyrosinase and phenoloxidases, participate in the formation of the periostracum. In the present study, we cloned a tyrosinase gene from Crassostrea angulata named Ca-tyrA1, and its potential function in early larval shell biogenesis was investigated. The Ca-tyrA1 gene has a full-length cDNA of 2430bp in size, with an open reading frame of 1896bp in size, which encodes a 631-amino acid protein that includes a 24-amino acid putative signal peptide...
January 17, 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28107905/a-multi-walled-carbon-nanotubes-based-molecularly-imprinted-polymers-electrochemical-sensor-for-the-sensitive-determination-of-hiv-p24
#8
Ya Ma, Xiao-Lei Shen, Qiang Zeng, Hai-Shui Wang, Li-Shi Wang
To develop a rapid, simple and sensitive method for the determination of human immunodeficiency virus p24 (HIV-p24), a novel molecularly imprinted polymers (MIPs) electrochemical sensor was constructed on the surface of a multi-walled carbon nanotubes (MWCNTs) modified glassy carbon electrode (GCE) by surface polymerization using acrylamide (AAM) as functional monomer, N,N'-methylenebisacrylamide (MBA) as cross-linking agent and ammonium persulphate (APS) as initiator. Each modification step was carefully examined by cyclic voltammetry (CV), differential pulse voltammetry (DPV) and scanning electron microscope (SEM)...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107616/superhydrophilic-antireflective-periodic-mesoporous-organosilica-coating-on-flexible-polyimide-substrate-with-strong-abrasion-resistance
#9
Jing Wang, Cong Zhang, Chunming Yang, Ce Zhang, Mengchao Wang, Jing Zhang, Yao Xu
Superhydrophilic antireflective periodic mesoporous organosilica (PMO) coating was prepared on flexible polyimide substrate via solvent-evaporation-induced self-assembly (SEISA) method, in which tetraethoxysilane (TEOS) and a special bridged silsesquioxane were used as reactants. The bridged silsesquioxane, EG-BSQ, was synthesized through the stoichiometric reaction between 3-glycidoxyporpyltrimethoxysilane (GPTMS) and ethylene diamine (EDA) at 60 (○)C with no catalyst. Under the influence of surfactant, TEOS and EG-BSQ co-condensed and enclosed the ordered mesporous in the coating...
January 20, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28107419/a-new-israeli-tobamovirus-isolate-infects-tomato-plants-harboring-tm-22-resistance-genes
#10
Neta Luria, Elisheva Smith, Victoria Reingold, Ilana Bekelman, Moshe Lapidot, Ilan Levin, Nadav Elad, Yehudit Tam, Noa Sela, Ahmad Abu-Ras, Nadav Ezra, Ami Haberman, Liron Yitzhak, Oded Lachman, Aviv Dombrovsky
An outbreak of a new disease infecting tomatoes occurred in October-November 2014 at the Ohad village in Southern Israel. Symptomatic plants showed a mosaic pattern on leaves accompanied occasionally by narrowing of leaves and yellow spotted fruit. The disease spread mechanically and rapidly reminiscent of tobamovirus infection. Epidemiological studies showed the spread of the disease in various growing areas, in the South and towards the Southeast and Northern parts of the country within a year. Transmission electron microscope (TEM) analysis showed a single rod-like form characteristic to the Tobamovirus genus...
2017: PloS One
https://www.readbyqxmd.com/read/28106983/two-dimensional-sns-a-phosphorene-analogue-with-strong-in-plane-electronic-anisotropy
#11
Zhen Tian, Chenglei Guo, Mingxing Zhao, Ranran Li, Jiamin Xue
We study the anisotropic electronic properties of two-dimensional (2D) SnS, an analogue of phosphorene, grown by physical vapor transport. With transmission electron microscope and polarized Raman spectroscopy, we identify the zigzag and armchair directions of the as-grown 2D crystals. 2D SnS field-effect transistors with a cross-Hall-bar structure are fabricated. They show heavily hole-doped (~10(19) cm(-3)) conductivity with strong in-plane anisotropy. At room temperature the mobility along the zigzag direction exceeds 20 cm(2)V(-1)s(-1), which can be up to 1...
January 20, 2017: ACS Nano
https://www.readbyqxmd.com/read/28106833/influence-of-temperature-on-transdermal-penetration-enhancing-mechanism-of-borneol-a-multi-scale-study
#12
Qianqian Yin, Ran Wang, Shufang Yang, Zhimin Wu, Shujuan Guo, Xingxing Dai, Yanjiang Qiao, Xinyuan Shi
The influence of temperature on the transdermal permeation enhancing mechanism of borneol (BO) was investigated using a multi-scale method, containing a coarse-grained molecular dynamic (CG-MD) simulation, an in vitro permeation experiment, and a transmission electron microscope (TEM) study. The results showed that BO has the potential to be used as a transdermal penetration enhancer to help osthole (OST) penetrate into the bilayer. With the increasing temperature, the stratum corneum (SC) becomes more flexible, proving to be synergistic with the permeation enhancement of BO, and the lag time (TLag) of BO and OST are shortened...
January 19, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28106390/tubulin-binding-and-polymerization-promoting-properties-of-tppp-proteins-are-evolutionarily-conserved
#13
Judit Oláh, Tibor Szénási, Adél Szabó, Kinga Kovács, Péter Lőw, Mauro Stifanic, Ferenc Orosz
Tubulin Polymerization Promoting Proteins (TPPPs) constitute a new eukaryotic protein family. There are three TPPP paralogs in the human genome; denoted as TPPP1, TPPP2 and TPPP3. TPPP1 and TPPP3 are intrinsically unstructured proteins (IUPs) that bind and polymerize tubulin and stabilize microtubules but TPPP2 does not. Vertebrate TPPPs originated from the ancient invertebrate TPPP by two-round whole-genome-duplication thus it was an open question whether the tubulin/microtubule binding function of TPPP1 and TPPP3 is a newly acquired property or it was present in the invertebrate orthologs (generally one TPPP/species)...
January 20, 2017: Biochemistry
https://www.readbyqxmd.com/read/28106371/enhancement-of-electrode-stability-using-platinum-cobalt-nanocrystals-on-a-novel-composite-sictic-support
#14
María Millán, Hector Zamora, Manuel Andrés Rodrigo, Justo Lobato
PtCo alloy catalysts for high temperature PEMFCs (Protonic Exchange Membrane Fuel Cells) were synthesized on a novel non-carbonaceous support (SiCTiC) using the impregnation method with NaBH4 as the reducing agent at different synthesis temperatures to evaluate the effect of this variable on their physicochemical and electrochemical properties. The catalysts were characterized by inductively coupled plasma optical emission spectrometry (ICP-OES), scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDX), X-ray diffraction (XRD), transmission electron microscope-energy dispersive X-ray (TEM) and temperature programmed reduction (TPR)...
January 20, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28104521/endoscopic-versus-microscopic-transsphenoidal-surgery-in-the-treatment-of-pituitary-adenoma-a-systematic-review-and-meta-analysis
#15
Aijun Li, Weisheng Liu, Peicheng Cao, Yuehua Zheng, Zhenfu Bu, Tao Zhou
BACKGROUND: Inconsistent findings have been reported regarding the efficacy and safety of endoscopic and microscopic transsphenoidal surgery for pituitary adenoma. This study aimed to assess the benefits and shortcomings of these surgical methods in patients with pituitary adenoma. METHODS: The electronic databases PubMed, EmBase, and the Cochrane Library were systematically searched, as well as proceedings of major meetings. Eligible studies with retrospective or prospective design that evaluated endoscopic versus microscopic methods in patients with pituitary adenoma were included...
January 16, 2017: World Neurosurgery
https://www.readbyqxmd.com/read/28104477/enantioselective-oxidative-stress-and-oxidative-damage-caused-by-rac-%C3%A2-and-s-metolachlor-to-scenedesmus-obliquus
#16
Huijun Liu, YiLu Xia, Weidan Cai, Yina Zhang, Xiaoqiang Zhang, Shaoting Du
The rational use and environmental security of chiral pesticides has gained the interest of many researchers. The enantioselective effects of Rac- and S-metolachlor on oxidative stress in Scenedesmus obliquus were determined in this study. Stronger green fluorescence was observed in response to S-metolachlor treatment than to Rac-metolachlor treatment, suggesting that more reactive oxygen species (ROS) were stimulated by S-metolachlor. ROS levels following S-metolachlor treatment were 1.92-, 8.31-, and 1.08-times higher than those observed following Rac-metolachlor treatment at 0...
January 5, 2017: Chemosphere
https://www.readbyqxmd.com/read/28104322/effect-from-rotational-speed-on-torsional-resistance-of-the-nickel-titanium-instruments
#17
Jung-Hong Ha, Sang Won Kwak, Sung Kyo Kim, Asgeir Sigurdsson, Hyeon-Cheol Kim
INTRODUCTION: The purpose of this study was to evaluate differences in torsional resistance using up-regulated speed of rotational spindle. METHODS: Three NiTi rotary instrument systems were selected in this study: K3XF (SybronEndo, Glendora, CA), BLX (B&L Biotech, Ansan, Korea), and OneShape (MicroMega, Besançon, France). The tip size and taper for all files were #25 and 0.06. Experimental groups (n = 10/group) were assigned to 2-, 60-, 350-, and 600-rpm groups by the rotational speed of spindle...
January 16, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28104018/-the-ultrastructure-of-utricular-maculae-of-mouse
#18
L J Yang, L J Li, T Liu, L W Wang, P Shen
Objective: Using scanning electron microscope to observe the ultrastructure of utricular maculae of mouse. Methods: Ten young (6 to 8 weeks) and ten old (>12 months) mice were executed, and their utricles were harvested and the specimens were processed, using scanning electron microscope to observe the structures of the utricles from the surface of otoconia layer to the roots of hair cell cilia. Results: Under the scanning electron microscope, several ultrastructures were observed, including otoconia layer, unstructured gelatinous extracellular matrix layer, honeycomb-like gelatinous extracellular matrix layer, inter-cilia otoconia and hair cell cilia associated with these structures...
January 7, 2017: Zhonghua Er Bi Yan Hou Tou Jing Wai Ke za Zhi, Chinese Journal of Otorhinolaryngology Head and Neck Surgery
https://www.readbyqxmd.com/read/28103990/-effects-of-human-amniotic-epithelial-stem-cells-derived-exosomes-on-healing-of-wound-with-full-thickness-skin-defect-in-rats
#19
B Zhao, G F Wu, Y J Zhang, W Zhang, F F Yang, D Xiao, K X Zeng, J H Shi, L L Su, D H Hu
Objective: To investigate the effects of human amniotic epithelial stem cells-derived exosomes on healing of wound with full-thickness skin defect in rats. Methods: (1) Human amniotic epithelial stem cells were isolated from the amnion tissue of 5 full-term pregnant women in Department of Obstetrics of our hospital by the method of trypsin digestion, and their morphology was observed. The third passage of cells were stained with rhodamine-phalloidin for cytoskeleton observation. The third passage of cells were identified with flow cytometry through the detection of expressions of cell surface markers CD29, CD31, CD34, CD90, CD105, SSEA3, SSEA4 and immunity-related marker human leukocyte antigen-D related site (HLA-DR)...
January 20, 2017: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
https://www.readbyqxmd.com/read/28103989/-experimental-research-on-the-effects-of-different-activators-on-the-formation-of-platelet-rich-gel-and-the-release-of-bioactive-substances-in-human-platelet-rich-plasma
#20
Y Yang, W Zhang, B Cheng
Objective: To explore the effects of calcium gluconate and thrombin on the formation of platelet-rich gel (PRG) and the release of bioactive substances in human platelet-rich plasma (PRP) and the clinical significance. Methods: Six healthy blood donors who met the inclusion criteria were recruited in our unit from May to August in 2016. Platelet samples of each donor were collected for preparation of PRP. (1) PRP in the volume of 10 mL was collected from each donor and divided into thrombin activation group (TA, added with 0...
January 20, 2017: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
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