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https://www.readbyqxmd.com/read/28214874/heat-acclimation-regulates-the-autophagy-lysosome-function-to-protect-against-heat-stroke-induced-brain-injury-in-mice
#1
Junfeng Yi, Genlin He, Ju Yang, Zhen Luo, Xuesen Yang, Xue Luo
BACKGROUND/AIMS: The mechanisms underlying the protective role of heat acclimation (HA) in heat stroke (HS)-induced brain injury are still unclear. The autophagy-lysosome pathway is known to pay an important role in protecting stressed or diseased cells from death. Nevertheless, whether autophagy and lysosomes are involved in HA-mediated neuroprotection following HS exposure remains unclear. METHODS: The protective effects of HA were assessed by rectal temperature, hematoxylin-eosin staining, transmission electron microscopic analysis, terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling staining, and Fluoro Jade B staining, after mice were subjected to HS...
January 18, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28214861/sirt1-regulates-the-inflammatory-response-of-vascular-adventitial-fibroblasts-through-autophagy-and-related-signaling-pathway
#2
Wei-Rong Wang, Ting-Ting Li, Ting Jing, Yan-Xiang Li, Xiao-Feng Yang, Yan-Hao He, Wei Zhang, Rong Lin, Ji-Ye Zhang
BACKGROUND/AIMS: Autophagy is a lysosomal degradation pathway that is essential for cellular survival, differentiation, and homeostasis. Sirtuin 1 (SIRT1), a NAD+-dependent deacetylase, plays a pivotal role in modulation of autophagy. Recent studies found that autophagy was involved in the regulation of inflammatory response. In this study, we aimed to determine the effect of SIRT1 on autophagy and inflammation, and whether autophagy can regulate the inflammatory response in vascular adventitial fibroblasts (VAFs)...
February 3, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28214756/multifarious-applications-of-atomic-force-microscopy-in-forensic-science-investigations
#3
REVIEW
Gaurav Pandey, Maithri Tharmavaram, Deepak Rawtani, Sumit Kumar, Y Agrawal
Forensic science is a wide field comprising of several subspecialties and uses methods derived from natural sciences for finding criminals and other evidence valid in a legal court. A relatively new area; Nano-forensics brings a new era of investigation in forensic science in which instantaneous results can be produced that determine various agents such as explosive gasses, biological agents and residues in different crime scenes and terrorist activity investigations. This can be achieved by applying Nanotechnology and its associated characterization techniques in forensic sciences...
February 11, 2017: Forensic Science International
https://www.readbyqxmd.com/read/28214533/the-effect-and-mechanism-of-inhibiting-glucose-6-phosphate-dehydrogenase-activity-on-the-proliferation-of-plasmodium-falciparum
#4
Zhiqiang Zhang, Xiaodan Chen, Chengrui Jiang, Zishui Fang, Yi Feng, Weiying Jiang
We screened >40,000 patients with glucose-6-phosphate dehydrogenase (G6PD) deficiency and found that the G6PD Kaiping allele was under the most positive selection for fighting against malaria in the Chinese population. However, the mechanism is unknown. The current study was designed to investigate the anti-malarial effect and mechanism of G6PD deficiency. Dehydroepiandrosterone (DHEA) was utilised for inhibiting the G6PD activity of erythrocytes. Giemsa staining of blood smears and quantitative real-time PCR were used for the detection and quantification of Plasmodium falciparum infection...
February 15, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28213963/ultrastructure-of-compatible-and-incompatible-interactions-in-phloem-sieve-elements-during-the-stylet-penetration-by-cotton-aphids-in-melon
#5
Elisa Garzo, Mercedes Fernández-Pascual, Cesar Morcillo, Alberto Fereres, M Luisa Gómez-Guillamón, W Fred Tjallingii
Resistance of the melon line TGR-1551 to the aphid Aphis gossypii is based on preventing aphids from ingesting phloem sap. In electrical penetration graphs (EPGs), this resistance has been characterized with A. gossypii showing unusually long phloem salivation periods (waveform E1) mostly followed by pathway activities (waveform C) or if followed by phloem ingestion (waveform E2), ingestion was not sustained for more than 10 minutes. Stylectomy with aphids on susceptible and resistant plants was performed during EPG recording while the stylet tips were phloem inserted...
February 18, 2017: Insect Science
https://www.readbyqxmd.com/read/28213258/p-aminothiophenol-coated-cdse-zns-quantum-dots-as-a-turn-on-fluorescent-probe-for-ph-detection-in-aqueous-media
#6
Dong Li, Hu Xu, Dan Li, Yuhong Wang
Since controlling hydrogen ion concentration (i.e. pH) is fairly important in various fields, developing a facile pH detection method is highly desired. Owing to the superior optical properties, semiconductor quantum dots (QDs) have been developed as a promising alternative for organic fluorophores in fluorescence analysis. In this study, a hydrophilic p-aminothiophenol (pATP) capped CdSe/ZnS QDs (denoted as pATP-QDs) as a selective "turn-on" fluorescence probe for pH in aqueous media is reported. The pATP-QDs probe easily obtained via ligand exchange are characterized by various analytic techniques including ultraviolet-visible, fourier transform infrared, fluorescence, time-resolved fluorescence spectroscopies, and transmission electron microscope etc...
May 1, 2017: Talanta
https://www.readbyqxmd.com/read/28213232/analysis-of-flavors-and-fragrances-by-hplc-with-fe3o4-go-magnetic-nanocomposite-as-the-adsorbent
#7
Rui Xiao, Xiaoting Zhang, Xiaona Zhang, Jiahua Niu, Minghua Lu, Xiuhua Liu, Zongwei Cai
In this article, a method for the analysis of flavors and fragrances including ethyl vanillin, trans-cinnamic acid, methyl cinnamate, ethyl cinnamate, and benzyl cinnamate in foods by HPLC with Fe3O4@GO nanocomposite as the adsorbent of magnetic solid-phase extraction was developed. The magnetic Fe3O4@GO nanocomposite was prepared and characterized by transmission electron microscope, infrared spectroscopy, X-ray diffraction and vibrating sample magnetometer. The as-prepared Fe3O4@GO nanocomposite was used as adsorbent for extraction and preconcentration of flavors and fragrances in foods...
May 1, 2017: Talanta
https://www.readbyqxmd.com/read/28211931/the-dynamic-conductance-response-and-mechanics-modulated-memristive-behavior-of-the-azurin-monolayer-under-cyclic-loads
#8
Xiaoyue Zhang, Jian Shao, Yun Chen, Weijin Chen, Jing Yu, Biao Wang, Yue Zheng
Azurin (Az) has been considered as the research hotspot in molecular electronics, as well as a promising material for building functional devices on the molecular scale because of its special electrical properties and force-dependent conductance effects. Here we carry out an in-depth investigation combined with molecular scale experiments, molecular dynamics simulations, first-principles calculations and theoretical models to reveal the dynamic conductance response of the Az monolayer under cyclic mechanical loading...
February 17, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28211724/experimental-evidence-for-static-charge-density-waves-in-iron-oxypnictides
#9
A Martinelli, P Manfrinetti, A Provino, A Genovese, F Caglieris, G Lamura, C Ritter, M Putti
In this Letter we report high-resolution synchrotron x-ray powder diffraction and transmission electron microscope analysis of Mn-substituted LaFeAsO samples, demonstrating that a static incommensurate modulated structure develops across the low-temperature orthorhombic phase, whose modulation wave vector depends on the Mn content. The incommensurate structural distortion is likely originating from a charge-density-wave instability, a periodic modulation of the density of conduction electrons associated with a modulation of the atomic positions...
February 3, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/28210641/atypical-plasmacytic-proliferation-in-a-case-of-c3-glomerulopathy-pathophysiology-demystified
#10
Osama Elfituri, Nathan Aardsma, Suman Setty, Frederick Behm, Kimberly Czech
An 11-year-old Hispanic female underwent evaluation of asymptomatic proteinuria and hematuria. The patient denied fever, edema, and gross hematuria. Urinalysis showed mild proteinuria, and a urine microscopic examination revealed red blood cells. Screening tests for glomerulonephritis revealed a low C3 and negative ANA, ASO, DNAse-B, and ANCA. Histological examination of a renal biopsy specimen showed glomeruli with endocapillary proliferation, a predominant C3 deposition in the capillary loops by immunofluorescence, and electron dense deposits in the mesangium, paramesangium, and capillary walls by electron microscopy consistent with a diagnosis of C3 glomerulopathy...
January 2017: Journal of Investigative Medicine High Impact Case Reports
https://www.readbyqxmd.com/read/28210458/osseous-oral-hyaline-ring-granuloma-mimicking-a-mandible-tumor-in-a-child-with-congenital-agenesis-of-the-corpus-callosum
#11
Rodrigo Neves-Silva, Camilla-Borges Ferreira-Gomes, Natalia Palmier, Marcelo Brum-Corrêa, Oslei Paes-Almeida, Marcio Ajudarte-Lopes, Pablo Agustin-Vargas, Alan-Roger Santos-Silva
BACKGROUND: Hyaline ring granuloma (HRG) of the oral cavity is an uncommon disorder considered to be a foreign-body reaction resulting from implantation of food vegetable particles. Microscopically, it is characterized by the presence of structures of hyaline rings in an inflamed fibrous tissue background, which contains multinucleated giant cells. MATERIAL AND METHODS: We present the case of a 4-year-old boy diagnosed with a mandible osseous HRG, which showed clinical and tomographic aspects suggestive of an aggressive bone tumor...
February 2017: Journal of Clinical and Experimental Dentistry
https://www.readbyqxmd.com/read/28210242/biocidal-inactivation-of-lactococcus-lactis-bacteriophages-efficacy-and-targets-of-commonly-used-sanitizers
#12
Stephen Hayes, James Murphy, Jennifer Mahony, Gabriele A Lugli, Marco Ventura, Jean-Paul Noben, Charles M A P Franz, Horst Neve, Arjen Nauta, Douwe Van Sinderen
Lactococcus lactis strains, being intensely used in the dairy industry, are particularly vulnerable to members of the so-called 936 group of phages. Sanitization and disinfection using purpose-made biocidal solutions is a critical step in controlling phage contamination in such dairy processing plants. The susceptibility of 36 936 group phages to biocidal treatments was examined using 14 biocides and commercially available sanitizers. The targets of a number of these biocides were investigated by means of electron microscopic and proteomic analyses...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28209381/an-easy-fast-and-low-tech-equipment-requiring-alternative-method-to-optimize-immunolabelling-conditions-for-pre-embedding-immunogold-electron-microscopy-and-to-correlate-light-and-electron-microscopical-immunogold-labelling-results
#13
Shweta Suiwal, Gabriele Kiefer, Frank Schmitz, Karin Schwarz
Correlating light microscopic immunolabelling results with electron microscopic data is of great interest in many fields of biomedical research but typically requires very specialized, expensive equipment and complex procedures which are not available in most labs. In this technical study, we describe an easy and "low-tech"-equipment-requiring pre-embedding immunolabelling approach that allows correlation of light microscopical immunolabelling results with electron microscopic (EM) data as demonstrated by the example of immunolabelled synaptic ribbons from retinal rod photoreceptor synapses...
February 13, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28209008/effect-of-resin-bonded-luting-agents-influencing-marginal-discrepancy-in-all-ceramic-complete-veneer-crowns
#14
Dhanraj Ganapathy, Anusha Sathyamoorthy, Hemalatha Ranganathan, Karthikeyan Murthykumar
INTRODUCTION: Marginal discrepancy severely affects the long term success of All ceramic complete veneer crowns. The precise role of resin luting agents influencing this phenomenon needs to be explored further. AIM: To estimate and compare the marginal discrepancy in CAD/CAM processed All ceramic complete veneer crowns prior and following luting with resin bonded luting agents. MATERIALS AND METHODS: Extracted human maxillary first premolars were randomly allocated into four groups of 27 samples each Viz...
December 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28206765/ultrafast-electronic-energy-transfer-in-an-orthogonal-molecular-dyad
#15
Christian Wiebeler, Felix Plasser, Gordon J Hedley, Arvydas Ruseckas, Ifor D W Samuel, Stefan Schumacher
Understanding electronic energy transfer (EET) is an important ingredient in the development of artificial photosynthetic systems and photovoltaic technologies. Although EET is at the heart of these applications and crucially influences their light-harvesting efficiency, the nature of EET over short distances for covalently bound donor and acceptor units is often not well understood. Here we investigate EET in an orthogonal molecular dyad (BODT4), in which simple models fail to explain the very origin of EET...
February 21, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/28205512/superplastic-behavior-of-silica-nanowires-obtained-by-direct-patterning-of-silsesquioxane-based-precursors
#16
Mustafa Yılmaz, Nicole Wollschläger, Mohammad Nasr Esfahani, Werner Österle, Yusuf Leblebici, B Erdem Alaca
Silica nanowires spanning 10 μm-deep trenches are fabricated from different types of silsesquioxane-based precursors by direct e-beam patterning on silicon followed by release through deep reactive ion etching. Nanowire aspect ratios as large as 150 are achieved with a critical dimension of about 50 nm and nearly rectangular cross-sections. In situ bending tests are carried out inside a scanning electron microscope, where the etch depth of 10 [Formula: see text] provides sufficient space for deformation. Silica NWs are indeed observed to exhibit superplastic behavior without fracture with deflections reaching the full etch depth, about two orders of magnitude larger than the nanowire thickness...
February 16, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28203012/-effects-of-saliva-contamination-on-bond-strength-of-resin-resin-interfaces
#17
H J Guo, C Z Gao, F Lin, W Liu, L Yue
OBJECTIVE: To estimate the bond strength between different resin composite interfaces, and to evaluate the effect of saliva contamination and management with the contamination on the bond strength. METHODS: Two commercial resin composites containing different types of fillers (AP-X: barium-glass; P60: quartz) were tested in this study. The basic composite blocks were made of AP-X. After wet-ground flatted, the experimental groups were divided into three groups according to the surface treatment: (1)Direct bonding group: the bonding surface was rinsed with distilled water for 20 s, then dried with oil-free air for 20 s; (2)Saliva contamination group: the bonding surface was bathed in saliva for 30 min, then distilled water was rinsed for 20 s and dried with oil-free air for 20 s; (3)Saliva contamination and 75% ethanol wiping group: bonding surface bathed in saliva for 30 min, then wipe the surface with 75% ethanol, distilled water rinse for 20 s and dry with oil-free air for 20 s; The control group was made of the basic composite bulks of AP-X directly bonding with composite P60...
February 18, 2017: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/28199862/effect-of-accelerating-voltage-on-beam-damage-of-asbestos-fibers-in-the-transmission-electron-microscope-tem
#18
Joannie Martin, Martin Beauparlant, Sébastien Sauvé, Gilles L'Espérance
Transmission electron microscopy (TEM) is a powerful and efficient tool for the analysis of asbestos fibers. Although this analysis technique is common and several standard methods exist for asbestos analysis, questions remain about the optimal conditions to be used. Because asbestos fibers are relatively sensitive to the electron beam, it is important to better understand the phenomena of damage in order to avoid them. This study specifically investigates the effect of the acceleration voltage on damage to four different types of asbestos fibers: chrysotile, amosite, crocidolite and anthophyllite...
January 25, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28199184/biphasic-organo-bioceramic-fibrous-composite-as-a-biomimetic-extracellular-matrix-for-bone-tissue-regeneration
#19
Sanjay Kumar, James A Stokes Iii, Derrick Dean, Christian Rogers, Elijah Nyairo, Vinoy Thomas, Manoj K Mishra
In bone tissue engineering, the organo-ceramic composite, electrospun polycaprolactone/hydroxyapatite (PCL/HA) scaffold has the potential to support cell proliferation, migration, differentiation, and homeostasis. Here, we report the effect of PCL/HA scaffold in tissue regeneration using human mesenchymal stem cells (hMSCs). We characterized the scaffold by fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), scanning electron microscope (SEM) and assessed its biocompatibility...
March 1, 2017: Frontiers in Bioscience (Elite Edition)
https://www.readbyqxmd.com/read/28199115/efficient-spin-flip-excitation-of-a-nickelocene-molecule
#20
Maider Ormaza, Nicolas Bachellier, Marisa N Faraggi, Benjamin Verlhac, Paula Abufager, Philippe Ohresser, Loïc Joly, Michelangelo Romeo, Fabrice Scheurer, Marie-Laure Bocquet, Nicolas Lorente, Laurent Limot
Inelastic electron tunneling spectroscopy (IETS) within the junction of a scanning tunneling microscope (STM) uses current-driven spin-flip excitations for an all-electrical characterization of the spin state of a single object. Usually decoupling layers between the single object, atom or molecule, and the supporting surface are needed in order to observe these excitations. Here we study the surface magnetism of a sandwich nickelocene molecule (Nc) adsorbed directly on Cu(100) by means of X-ray magnetic circular dichroism (XMCD) and density functional theory calculations (DFT), and show with IETS that it exhibits an exceptionally efficient spin-flip excitation...
February 15, 2017: Nano Letters
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