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https://www.readbyqxmd.com/read/28813641/aim2-inflammasome-activation-and-regulation-a-structural-perspective
#1
Bing Wang, Qian Yin
Absent in melanoma 2 (AIM2) inflammasome is a multi-protein platform that recognizes aberrant cytoplasmic dsDNA and induces cytokine maturation, release and pyroptosis. It is composed of AIM2, apoptosis-associated speck-like protein containing a CARD (ASC), and caspase-1. Recent X-ray crystallographic and high resolution cryo-electron microscopic (cryo-EM) studies have revealed a series of structures in AIM2 inflammasome activation and regulation. One prominent feature common in multiple steps is the assembly of high-order structures, especially helical filaments nucleated by upstream molecules, rather than stoichiometric complexes...
August 13, 2017: Journal of Structural Biology
https://www.readbyqxmd.com/read/28813167/lipophagy-maintains-energy-homeostasis-in-the-kidney-proximal-tubule-during-prolonged-starvation
#2
Satoshi Minami, Takeshi Yamamoto, Yoshitsugu Takabatake, Atsushi Takahashi, Tomoko Namba, Jun Matsuda, Tomonori Kimura, Jun-Ya Kaimori, Isao Matsui, Takayuki Hamano, Hiroaki Takeda, Masatomo Takahashi, Yoshihiro Izumi, Takeshi Bamba, Taiji Matsusaka, Fumio Niimura, Yoshitaka Isaka
Macroautophagy/autophagy is a self-degradation process that combats starvation. Lipids are the main energy source in kidney proximal tubular cells (PTCs). During starvation, PTCs increase fatty acid (FA) uptake, form intracellular lipid droplets (LDs), and hydrolyze them for use. The involvement of autophagy in lipid metabolism in the kidney remains largely unknown. Here, we investigated the autophagy-mediated regulation of renal lipid metabolism during prolonged starvation using PTC-specific Atg5-deficient (atg5-TSKO) mice and an in vitro serum starvation model...
August 16, 2017: Autophagy
https://www.readbyqxmd.com/read/28813122/morphological-alterations-in-the-jejunal-mucosa-of-aged-rats-and-the-possible-protective-role-of-green-tea
#3
Zeinab Abel-Rehim Hassan, Agata Zauszkiewicz-Pawlak, Shaimaa A Abdelrahman, Sami Algaidi, Maha Desouky, Sally M Shalaby
INTRODUCTION: Gastrointestinal disorders become more prevalent with ageing. This study is aimed to describe morphological changes that occur in the jejunal mucosa of male albino rats as a result of ageing and the protective effect of green tea supplements. MATERIAL AND METHODS: The experiment was performed on sixty rats: thirty young-adult (6-month old, body mass 200-220 g) and thirty old (24-month-old, body mass 220-260 g) animals. Each group was further divided into two subgroups (n = 15 each): control rats and green tea-treated rats that received 1...
August 16, 2017: Folia Histochemica et Cytobiologica
https://www.readbyqxmd.com/read/28812993/enhanced-corrosion-resistance-of-carbon-steel-in-hydrochloric-acid-solution-by-eriobotrya-japonica-thunb-leaf-extract-electrochemical-study
#4
Wenjing Yang, Qihui Wang, Ke Xu, Yanjun Yin, Hebin Bao, Xueming Li, Lidan Niu, Shiqi Chen
The biodegradable inhibitors, which could effectively reduce the rate of corrosion of carbon steel, were investigated by potentiodynamic polarization and electrochemical impedance spectroscopy (EIS). The mixed-type inhibitors extracted from Eriobotrya japonica Thunb. leaf exhibited excellent inhibition performance, and the inhibition efficiency for carbon steel reached 90.0% at 298 K in hydrochloric acid. Moreover, the adsorption mechanism of the inhibitors on a carbon steel surface is described by the Langmuir adsorption isotherm...
August 16, 2017: Materials
https://www.readbyqxmd.com/read/28812227/effects-of-fluoride-on-surface-structure-of-primary-culture-leydig-cells-in-mouse
#5
Jian Li, Yan Shi, Hua Fan, Yanyan Li, Yuchen Zhu, Xijun Lin, Jianhai Zhang
Fluoride (F) is known to induce reproduction toxicity, and the elucidation of its underlying mechanisms is an ongoing research. These findings aim to provide deeper insights into roles of soduim fluoride (NaF) in testis damage, which could contribute to a better understanding of fluoride-induced male reproductive toxicity. The Leydig cells were administrated by 0, 5, 10, and 20 mg/L NaF for 24 h, respectively. Scanning electron microscope was used to identify the change of surface structure in the Leydig cells...
August 15, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/28811526/observation-of-the-unexpected-morphology-of-graphene-wrinkle-on-copper-substrate
#6
Wen Wang, Shudu Yang, Ashu Wang
Graphene, a two-dimensional material, has a wide range of unique properties and could be used in the development of varieties of mechanic, electronic and photonic devices, therefore methods to synthesis large-area high-quality graphene films are urgently required. Chemical vapor deposition (CVD) has been of particular interest recently due to its simplicity and low cost. However, because of the mismatch of thermal expansion coefficients, high densities of wrinkles are commonly observed. Despite their prevalence and potential impact on large-scale graphene properties, relatively little is known about their structural morphology and formation mechanism...
August 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28811371/angular-reconstitution-based-3d-reconstructions-of-nanomolecular-structures-from-superresolution-light-microscopy-images
#7
Desirée Salas, Antoine Le Gall, Jean-Bernard Fiche, Alessandro Valeri, Yonggang Ke, Patrick Bron, Gaetan Bellot, Marcelo Nollmann
Superresolution light microscopy allows the imaging of labeled supramolecular assemblies at a resolution surpassing the classical diffraction limit. A serious limitation of the superresolution approach is sample heterogeneity and the stochastic character of the labeling procedure. To increase the reproducibility and the resolution of the superresolution results, we apply multivariate statistical analysis methods and 3D reconstruction approaches originally developed for cryogenic electron microscopy of single particles...
August 15, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28810900/polymer-free-dual-drug-eluting-stents-evaluated-in-a-porcine-model
#8
Bin Zhang, Bo Zheng, Xingang Wang, Qiuping Shi, Jia Jia, Yong Huo, Chunshui Pan, Jingyan Han, Ming Chen
BACKGROUND: Although drug-eluting stents have dramatically reduced the rates of restenosis and target lesion revascularization, they are associated with stent thrombosis (ST), a catastrophic event likely due to delayed endothelialization and chronic inflammation caused by the polymer and the metal scaffolds. To increase the safety and efficacy of stents, polymer-free dual drug-eluting stents (DDES) have been developed. METHODS: A total 160 stents (Bare-metal stents (BMS), polymer-free probucol stents (PrES), sirolimus-eluting stents (SES) and DDES) were randomly implanted in the coronary arteries of 80 pigs...
August 15, 2017: BMC Cardiovascular Disorders
https://www.readbyqxmd.com/read/28810223/effects-of-an-allelochemical-in-phaeodactylum-tricornutum-filtrate-on-heterosigma-akashiwo-morphological-physiological-and-growth-effects
#9
Rui Wang, Qiaona Xue, Jiangtao Wang, Liju Tan, Qingchun Zhang, Yue Zhao, Donald M Anderson
The effects of an allelochemical extracted from the culture filtrate of diatom Phaeodactylum tricornutum on the raphidophyte Heterosigma akashiwo were investigated using a series of morphological, physiological and biochemical characters. Growth experiments showed that H. akashiwo was significantly inhibited immediately after exposure to the allelochemical, with many cells rapidly dying and lysing based on microscopic observation. The effects of the allelochemical on the surviving cells were explored using Scanning Electron Microscopy (SEM) and Flow cytometry (FCM), the latter by examination of a suite of physiological parameters (membrane integrity, esterase activity, chlorophyll-a content, membrane potential)...
August 7, 2017: Chemosphere
https://www.readbyqxmd.com/read/28809996/submammary-granular-parakeratosis-treated-with-mastopexy
#10
Garrett Nelson, Mary H Lien, Jane L Messina, Sonali Ranjit, Neil Alan Fenske
<p>Granular parakeratosis, originally named axillary granular parakeratosis, is an uncommon disease with an unclear etiology. It is thought to result from defective processing of profillagrin to fillagrin, causing retention of keratohyaline granules in the epidermis. A myriad of causative factors has been proposed, including friction, moisture, heat, and contact irritants such as deodorants. We present a case in the inframammary area that resolved with mastopexy, further supporting the role of friction, moisture, and heat...
August 1, 2017: Journal of Drugs in Dermatology: JDD
https://www.readbyqxmd.com/read/28809468/construction-of-supramolecular-drug-drug-delivery-system-for-non-small-cell-lung-cancer-therapy
#11
Zhihao Zhang, Leilei Shi, Chenwei Wu, Yue Su, Jiwen Qian, Hongping Deng, Xinyuan Zhu
Nanoscale drug delivery systems (DDSs) are generally considered to be an effective alternative to small molecular chemotherapeutics due to the improved accumulation in tumor site and enhanced retention in blood. Nevertheless, most of DDSs have low loading efficiency or even pose high threat to normal organs caused by severe side effects. Ideally, supramolecular drug-drug delivery system (SDDDS) made up of pure drugs via supramolecular interaction provides a hopeful solution for cancer treatment. Herein, we put forward a facile method to construct SDDDS via co-assembly of Gefitinib (GEF) and tripeptide tyroservatide (YSV), two kinds of chemotherapeutic pharmaceuticals for non-small cell lung cancer (NSCLC), via multiple intermolecular interactions, including hydrogen bond and π-π stacking...
August 15, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28809107/synthesis-and-insecticidal-activity-of-enzyme-triggered-functionalized-hollow-mesoporous-silica-for-controlled-release
#12
Amir Esmat Kaziem, Yunhao Gao, Shun He, Jianhong Li
In the present study, enzymatic responsive controlled release formulations (CRFs) were fabricated. The CRFs were achieved by anchoring mechanically interlocked molecules using α-cyclodextrin onto the surface pore rims of hollow mesoporous silica (HMS). The CRFs were characterized using Fourier transform infrared spectroscopy, scanning electron microscope, transmission electron microscope, and thermogravimetric analysis. The results showed that the CRFs had extraordinary loading ability for chlorantraniliprole (42% w/w) and could effectively preserve chlorantraniliprole against degradation under thermal conditions and UV radiation...
August 15, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28808811/construction-and-biocompatibility-of-a-thin-type-i-ii-collagen-composite-scaffold
#13
Long Han, Zhong-Wen Zhang, Bo-He Wang, Zhen-Kun Wen
Articular cartilage injury is a common type of damage observed in clinical practice. A matrix-induced autologous chondrocyte implant was developed to repair articular cartilage as an advancement on the autologous chondrocyte implant procedure. Here, we establish a thin double layer of collagen as a novel and effective bioscaffold for the regeneration of cartilaginous lesions. We created a collagen membrane with double layers using a cover slip, a cover slip, and the collagen was then freeze-dried under vacuum...
August 14, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28808700/effect-of-chlorhexidine-and-ethanol-on-microleakage-of-composite-resin-restoration-to-dentine
#14
Iman Ramezanian Nik, Ehsan Baradaran Naseri, Sara Majidinia, Sara Ramezanian Nik, Mohammad Jafari Giv
OBJECTIVE: To evaluate the effect of chlorhexidine and ethanol on microleakage of composite resin restoration to dentine. METHODS: Class II cavities with dentinal margin were prepared on 96 premolar teeth. All specimens were acid-etched, rinsed and dried. Then the samples were randomly divided into four groups according to pre-treatment of the dentine: no treatment (control group); treatment with 100% ethanol for 60 s (group 2); treatment with 2% chlorhexidine for 60 s (group 3); 100% ethanol for 60 s and then 2% chlorhexidine for 60 s (group 4)...
2017: Chinese Journal of Dental Research
https://www.readbyqxmd.com/read/28808635/effect-of-dentin-biomodifiers-on-the-immediate-and-long-term-bond-strengths-of-a-simplified-etch-and-rinse-adhesive-to-dentin
#15
Payal Singh, Rajni Nagpal, Udai Pratap Singh
OBJECTIVES: This in vitro study evaluated the effect of dentin biomodifiers on the immediate and long-term bond strengths of a simplified etch and rinse adhesive to dentin. MATERIALS AND METHODS: Flat coronal dentin surfaces were prepared in 120 extracted human molars. Teeth were randomly divided into 5 groups (n = 24) according to 5 different surface pre-treatments: No pre-treatment (control); 1M carbodiimide (EDC); 0.1% epigallocatechin-3-gallate (EGCG); 2% minocycline (MI); 10% sodium ascorbate (SA)...
August 2017: Restorative Dentistry & Endodontics
https://www.readbyqxmd.com/read/28808634/white-mineral-trioxide-aggregate-mixed-with-calcium-chloride-dihydrate-chemical-analysis-and-biological-properties
#16
Hany Mohamed Aly Ahmed, Norhayati Luddin, Thirumulu Ponnuraj Kannan, Khairani Idah Mokhtar, Azlina Ahmad
OBJECTIVES: This study aimed to evaluate the chemical and biological properties of fast-set white mineral trioxide aggregate (FS WMTA), which was WMTA combined with calcium chloride dihydrate (CaCl2·2H2O), compared to that of WMTA. MATERIALS AND METHODS: Surface morphology, elemental, and phase analysis were examined using scanning electron microscope (SEM), energy dispersive X-ray microanalysis (EDX), and X-ray diffraction (XRD), respectively. The cytotoxicity and cell attachment properties were evaluated on human periodontal ligament fibroblasts (HPLFs) using methyl-thiazol-diphenyltetrazolium (MTT) assay and under SEM after 24 and 72 hours, respectively...
August 2017: Restorative Dentistry & Endodontics
https://www.readbyqxmd.com/read/28808598/synthesis-characterization-and-adsorptive-properties-of-fe3o4-go-nanocomposites-for-antimony-removal
#17
Xiuzhen Yang, Tengzhi Zhou, Bozhi Ren, Zhou Shi, Andrew Hursthouse
A magnetic Fe3O4/GO composite with potential for rapid solid-liquid separation through a magnetic field was synthesized using GO (graphene oxide) and Fe3O4 (ferriferous oxide). Characterization of Fe3O4/GO used scanning electron microscope (SEM), X-ray diffractometer (XRD), Fourier transform infrared spectrometer (FT-IR), and Vibrating Sample Magnetometer (VSM). A number of factors such as pH and coexisting ions on adsorbent dose were tested in a series of batch experiments. The results showed that GO and Fe3O4 are strongly integrated...
2017: Journal of Analytical Methods in Chemistry
https://www.readbyqxmd.com/read/28808474/effect-of-shin-iseihaito-xinyiqingfeitang-on-acute-streptococcus-pneumoniae-murine-sinusitis-via-macrophage-activation
#18
Masaaki Minami, Toru Konishi, Hiroshi Takase, Zhixia Jiang, Tetsuya Arai, Toshiaki Makino
Streptococcus pneumoniae (S. pneumoniae) causes sinusitis. The general treatment of S. pneumonia sinusitis is by using antibiotics; however, one of their serious problems is the attenuation of their effect. Shin'iseihaito (Xinyiqingfeitang), a formula of Japanese traditional Kampo medicine, has been used for the treatment of sinusitis in Japan. In this study, we investigated the efficacy of Shin'iseihaito against S. pneumoniae-caused sinusitis in mice. Oral administration of Shin'iseihaito extract (SSHT) decreased the nasal colonization of S...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28808467/comparison-of-the-penetration-depth-of-conventional-and-nano-particle-calcium-hydroxide-into-dentinal-tubules
#19
Vahid Zand, Hadi Mokhtari, Aila Hasani, Golchin Jabbari
INTRODUCTION: The aim of this in vitro study was to evaluate and compare the penetration depth of conventional (CH) and nano-particle calcium hydroxide (NCH) into dentinal tubules. METHODS AND MATERIALS: Ninety human single-rooted teeth were instrumented by RaCe rotary system and after chemomechanical preparation were randomly divided in two equal groups (n=45). In the first group conventional CH and in the other NCH was used as intracanal medicament. After 2 weeks of incubation all roots were intentionally split at longitudinal axis and prepared for scanning electron microscope (SEM) observation...
2017: Iranian Endodontic Journal
https://www.readbyqxmd.com/read/28808388/caesalpinia-crista-linn-induces-protection-against-dna-and-membrane-damage
#20
R Sunil Kumar, Ramesh Balenahalli Narasingappa, Chandrashekhar G Joshi, Talakatta K Girish, Ananda Danagoudar
BACKGROUND: Caesalpinia crista is a medicinal herb used to cure various ailments in subtropical and tropical regions of Southeast Asia. OBJECTIVE: The objective of this evaluation of C. crista against free radical induced DNA and erythrocyte damage. MATERIALS AND METHODS: The profiles of polyphenol and flavonoid were quantified through reversed-phase high-performance liquid chromatography. Free radical induced DNA and membrane damage were performed using H2O2 as oxidative agent...
July 2017: Pharmacognosy Magazine
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