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Liyan Kong, Jiaming Zhu, Wen Wang, Lehe Jin, Yanjiao Fu, Bohui Duan, Liang Tan
As an integral glycoprotein on the surface of endothelial cells, thrombomodulin (TM) has very high affinity for thrombin. TM has been regarded to be a marker of endothelial damage since it can be released during endothelial cell injury. In this work, a highly sensitive fluorescence method for the quantitative detection of TM was developed. TM antibody (Ab) and bovine serum albumin (BSA) were bound on gold nanoparticles (AuNPs) to construct BSA-AuNPs-Ab nanocomposites and they were characterized by transmission electron microscope and UV-vis spectrophotometry...
October 17, 2016: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Mitsuaki Kojima, Joao Antonio Gimenes-Junior, Simone Langness, Koji Morishita, Ophelie Lavoie-Gagne, Brian Eliceiri, Todd W Costantini, Raul Coimbra
BACKGROUND: Previous studies have shown that mesenteric lymph (ML) has a crucial role in driving the systemic inflammatory response after trauma/hemorrhagic shock (T/HS). The specific mediators in the ML that contribute to its biological activity remain unclear despite decades of study. Exosomes are extracellular vesicles that are shed into body fluids such as serum and urine that can mediate intercellular communication. We hypothesized that exosomes are present in the ML after trauma/shock and are responsible for the biological activity of ML...
October 25, 2016: Journal of Trauma and Acute Care Surgery
D-X Ban, Y Liu, T-W Cao, S-J Gao, S-Q Feng
STUDY DESIGN: The rat's acellular spinal cord scaffold (ASCS) and spinal cord neurons were prepared in vitro to explore their biocompatibility. OBJECTIVES: The preparation of ASCS and co-culture with neuron may lay a foundation for clinical treatment of spinal cord injury (SCI). SETTING: Tianjin Medical University General Hospital, ChinaMethods:ASCS was prepared by chemical extraction method. Hematoxylin and eosin (H&E), myelin staining and scanning electron microscope were used to observe the surface structure of ASCS...
October 25, 2016: Spinal Cord
Jianbo Tan, Xuechao Zhang, Dongdong Liu, Yuhao Bai, Chundong Huang, Xueliang Li, Li Zhang
Carbon dioxide (CO2 )-responsive polymer nano-objects are prepared by photoinitiated reversible addition-fragmentation chain transfer dispersion polymerization of 2-hydroxypropyl methacrylate and 2-(dimethylamino)ethyl methacrylate (DMAEMA) in water at room temperature using a poly(poly(ethylene glycol) methyl ether methacrylate) macromolecular chain transfer agent. Kinetic studies confirm that full monomer conversions are achieved in all cases within 10 min of visible-light irradiation (405 nm, 0.5 mW cm(-2) )...
October 25, 2016: Macromolecular Rapid Communications
Shun-Jin Hu, Bing Shu, Hua Jin, Xiao-Feng Li, Jia-Rong Mao, Ke-Jun Ren, Lei Gao, Li Yang, You-Wan Wu, Yong-Jun Wang
OBJECTIVE: To evaluate the renal protective effect of Tangshenkang Granule () in a rat model of diabetic nephropathy (DN). METHODS: Forty male Sprague-Dawley rats were randomly divided into control, DN, Tangshenkang and benazepril groups. DN model was established in the rats of DN, Tangshenkang and benazepril groups. Tangshenkang Granule solution and benazepril hydrochloride solution were intragastrically administered daily to the rats in the Tangshenkang and benazepril groups for 8 weeks, respectively...
October 24, 2016: Chinese Journal of Integrative Medicine
Vassilios Atsaves, Maria G Detsika, Elpida Poulaki, Hara Gakiopoulou, Elias A Lianos
Although the protective role of HO-1 induction in various forms of kidney disease is well established, mechanisms other than heme catabolism to biliverdin, bilirubin and carbon monoxide have recently been identified. Unraveling these mechanisms requires the generation of appropriate animal models. The present study describes the generation of a HO-1 deficient Hmox1 (-/-) rat model and characterizes its renal and extrarenal phenotype. Hmox1 (-/-) rats had growth retardation and splenomegaly compared to their Hmox1 (+/+) littermates...
October 24, 2016: Transgenic Research
Catherine I Carty, Alison M Lee, Nathan A E Wienandt, Edward L Stevens, Derron A Alves, John A Browne, Jill Bryan, Eoin G Ryan, Joseph P Cassidy
BACKGROUND: An unusual presentation of skin disease was identified in two related neonatal Pedigree Limousin calves presented to University Veterinary Hospital, University College Dublin, following detailed post mortem examination a diagnosis of dermatosparaxis was made. Dermatosparaxis in animals or Ehlers Danlos Syndrome, which is the analogous condition seen in humans, is a connective tissue disorder characterised by extreme skin fragility. To the authors' knowledge this is the first report of such a diagnosis in the Limousin breed and the features of this lethal phenotype were severe in comparison to previous reports of the condition...
2016: Irish Veterinary Journal
Ji-Xia Chai, Hui-Hui Li, Yuan-Yuan Wang, Qiang Chai, Wen-Xin He, Yan-Mei Zhou, Xiao-Dong Hu, Zhen-Huan Wang
OBJECTIVE: To explore the effect of diallyl disulfide (DADS) on hippocampal synapses and learning and memory abilities in a mouse model of A1zheimer's disease (AD). METHODS: Mouse models of AD established by agglutinated Aβ1-42 injection in the lateral cerebral ventricle were randomized into 4 groups and treated with DADS at the daily doses of 0, 10, 50 and 100 mg/kg by gavage for 30 consecutive days. The learning and memory abilities of the mice were assessed with Morris water maze test; the structures of the dendritic spines and synapses in CA1 region of the hippocampus were observed under transmission electron microscope with silver staining; PSD95 and SYP protein and mRNA expressions in the hippocampus were detected with Western blotting and RT-PCR...
October 20, 2016: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
Kalyan Kong, Noriko Hiraishi, Mohannad Nassar, Masayuki Otsuki, Cynthia K Y Yiu, Junji Tagami
PURPOSE: Phytic acid (IP6) works well as an etchant in dentin bonding to remove the smear layer due to its acidity and chelating effect. This study compared the etching effect of IP6 with phosphoric acid (PA) and ethylenediaminetetraacetic acid (EDTA) on resin-dentin bond strength, micromorphology of the etched dentin surface and nanoleakage formation along resin-dentin interfaces and compared the protecting effect against collagen degradation. METHODS: Dentin disks and flat dentin surfaces were obtained from extracted human teeth...
October 21, 2016: Journal of Prosthodontic Research
Emmanuel João Nogueira Leal Silva, Laise Silva Villarino, Victor Talarico Leal Vieira, Thais Accorsi-Mendonça, Henrique Dos Santos Antunes, Gustavo De-Deus, Hélio Pereira Lopes
INTRODUCTION: This study evaluated the bending resistance and cyclic fatigue life of a new single-file reciprocating instrument (Unicone; Medin, Nové Město na Moravě, Czech Republic). Reciproc (VDW, Munich, Germany) and WaveOne (Dentsply Maillefer) instruments were used as references for comparison. METHODS: Flexibility was determined by 45° bending tests using a universal testing machine. The cyclic fatigue test was performed using a custom-made device. For this test, an artificial canal with a 60° angle and a 5-mm radius of curvature was used...
October 21, 2016: Journal of Endodontics
Athina M Mavridou, Esther Hauben, Martine Wevers, Evert Schepers, Lars Bergmans, Paul Lambrechts
INTRODUCTION: The aim of this study was to investigate the 3-dimensional (3D) structure and the cellular and tissue characteristics of external cervical resorption (ECR) in vital teeth and to understand the phenomenon of ECR by combining histomorphological and radiographic findings. METHODS: Twenty-seven cases of vital permanent teeth displaying ECR were investigated. ECR diagnosis was based on clinical and radiographic examination with cone-beam computed tomographic imaging...
October 21, 2016: Journal of Endodontics
Marco Aurélio Versiani, Fuad Jacob Abi Rached-Junior, Anil Kishen, Jesus Djalma Pécora, Yara Terezinha Silva-Sousa, Manoel Damião de Sousa-Neto
INTRODUCTION: Metallic antibacterial nanoparticles have been shown to provide distinct antibacterial advantage and antibiofilm efficacy when applied in infected root canals. The purpose of this study was to evaluate the influence of incorporating zinc oxide nanoparticles (ZnO-Np) on the physicochemical properties of Grossman sealer. METHODS: Grossman sealer was prepared according to its original formula. Additionally, 4 experimental sealers were prepared by replacing the zinc oxide component of the powder with ZnO-Np (average size of 20 nm) in different amounts (25%, 50%, 75%, and 100%)...
October 21, 2016: Journal of Endodontics
Haibo He, Ziqing Zhou, Chen Dong, Xin Wang, Qiong-Wei Yu, Yunyi Lei, Liqiang Luo, Yuqi Feng
A boronate-decorated nanomagnetic organic-inorganic hybrid material was facilely synthesized by utilizing the nanomagnetic polyhedral oligomeric silsesquioxanes (POSS) composite (Fe3O4@POSS) as the base platform. A simple copolymerization occurred between 3-acrylamidophenylboronic acid (AAPBA) and the residual end vinyl groups supplied by the substrate. Here the special emphasis was placed on the octavinyl POSS, which not only acted as the building blocks for a hybrid architecture but also facilitated the process of grafting boronate groups onto the surface of POSS based nanomagnetic composite (Fe3O4@POSS)...
November 9, 2016: Analytica Chimica Acta
Mathias Procop, Vasile-Dan Hodoroaba, Ralf Terborg, Dirk Berger
A method is proposed to determine the effective detector area for energy-dispersive X-ray spectrometers (EDS). Nowadays, detectors are available for a wide range of nominal areas ranging from 10 up to 150 mm2. However, it remains in most cases unknown whether this nominal area coincides with the "net active sensor area" that should be given according to the related standard ISO 15632, or with any other area of the detector device. Moreover, the specific geometry of EDS installation may further reduce a given detector area...
October 25, 2016: Microscopy and Microanalysis
Manjie Li, Zhaowei Liu, Yongcan Chen, Yang Hai
Interaction between old, corroded iron pipe surfaces and bulk water is crucial to the water quality protection in drinking water distribution systems (WDS). Iron released from corrosion products will deteriorate water quality and lead to red water. This study attempted to understand the effects of pipe materials on corrosion scale characteristics and water quality variations in WDS. A more than 20-year-old hybrid pipe section assembled of unlined cast iron pipe (UCIP) and galvanized iron pipe (GIP) was selected to investigate physico-chemical characteristics of corrosion scales and their effects on water quality variations...
October 18, 2016: Water Research
Quentin Jeangros, Martial Duchamp, Jérémie Werner, Maximilian Kruth, Rafal E Dunin-Borkowski, Björn Niesen, Christophe Ballif, Aïcha Hessler-Wyser
Changes in the nanostructure of methylammonium lead iodide (MAPbI3) perovskite solar cells are assessed as a function of current/voltage stimulus by biasing thin samples in situ in a transmission electron microscope. Various degradation pathways are identified both in situ and ex situ, predominantly at the positively biased MAPbI3 interface. Iodide migrates into the positively biased transport layer and also volatilises along with organic species, which triggers the nucleation of PbI2 nanoparticles and voids and hence decreases the cell performance...
October 24, 2016: Nano Letters
Beata Borowiak-Sobkowiak, Agnieszka Woźniak, Waldemar Bednarski, Magda Formela, Sławomir Samardakiewicz, Iwona Morkunas
The aim of this study was to investigate whether and to what extent oxidative stress is induced in leaves of one- and two-month-old plants of Asparagus officinalis L. cv. Argenteuil infested by Brachycorynella asparagi (Mordvilko) at a varied population size. The pest B. asparagi has been described as the most damaging species feeding on asparagus. Analyses using electron paramagnetic resonance (EPR) demonstrated generally higher concentrations of semiquinone radicals with g-values of 2.0045 ± 0.0005 and 2...
October 20, 2016: International Journal of Molecular Sciences
Y Lu, K Wang, F-R Chen, W Zhang, M L Sui
It has been fundamentally important and technologically challenging to elucidate the migration behavior of solute atoms in solvents, which can help to understand the growth of nanoparticles. Recently, ascribed to the booming development of start-of-the-art liquid environmental transmission electron microscopes (LETEMs), it has become possible to disclose, in situ, the phase segregation mechanism of elementary units in a solvent at the nanoscale. In addition, bombardment with an electron beam can induce a locally positive potential, with the application of low-conductive Si3N4 and water in LETEMs...
October 24, 2016: Physical Chemistry Chemical Physics: PCCP
Alexander Karabanov, Grzegorz Kwiatkowski, Carlo U Perotto, Daniel Wiśniewski, Jonathan McMaster, Igor Lesanovsky, Walter Köckenberger
A theory of dynamic nuclear polarisation (DNP) by thermal mixing is suggested based on purely quantum considerations. A minimal 6-level microscopic model is developed to test the theory and link it to the well-known thermodynamic model. Optimal conditions for the nuclear polarization enhancement and effects of inhomogeneous broadening of the electron resonance are discussed. Macroscopic simulations of nuclear polarization spectra displaying good agreement with experiments, involving BDPA and trityl free radicals, are presented...
October 24, 2016: Physical Chemistry Chemical Physics: PCCP
Reda Boulmène, Muthuramalingam Prakash, Majdi Hochlaf
Ab initio and DFT studies on CO2 interacting with different tautomers and isomers of triazole (TZ) are carried out to understand the adsorption mechanism and their mutual preferential sites. We used post Hartree-Fock methods (MP2, CCSD(T), and CCSD(T)-F12) and various DFTs (PBE, PBE0, M05-2X, and M11) with and without considering the dispersion correction for comparison. We determined hence the equilibrium structures, vibrational frequencies and binding energies of TZ-CO2 clusters and mapped their potential energy surfaces along the intermonomer coordinates...
October 24, 2016: Physical Chemistry Chemical Physics: PCCP
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