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https://www.readbyqxmd.com/read/28081290/membrane-microdomains-and-the-cytoskeleton-constrain-athir1-dynamics-and-facilitate-the-formation-of-an-athir1-associated-immune-complex
#1
Xueqin Lv, Yanping Jing, Jianwei Xiao, Yongdeng Zhang, Yingfang Zhu, Russell Julian, Jinxing Lin
Arabidopsis hypersensitive induced reaction (AtHIR) proteins function in plant innate immunity. However, the underlying mechanisms by which AtHIRs participate in plant immunity remain elusive. Here, using VA-TIRFM and FLIM-FRET, we revealed that AtHIR1 is present in membrane microdomains and co-localizes with the membrane microdomain marker REM1.3. Single-particle tracking analysis revealed that membrane microdomains and the cytoskeleton, especially microtubules, restrict the lateral mobility of AtHIR1 at the plasma membrane and facilitate its oligomerization...
January 12, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28081257/influence-of-la3-substitution-on-structure-morphology-and-magnetic-properties-of-nanocrystalline-ni-zn-ferrite
#2
Y K Dasan, B H Guan, M H Zahari, L K Chuan
Lanthanum substituted Ni-Zn ferrite nanoparticles (Ni0.5Zn0.5LaxFe1-xO4; 0.00 ≤x≤ 1.00) synthesized by sol-gel method were presented. X-ray diffraction patterns reveal the typical single phase spinel cubic ferrite structure, with the traces of secondary phase for lanthanum substituted nanocrystals. In addition, the structural analysis also demonstrates that the average crystallite size varied in the range of 21-25 nm. FTIR spectra present the two prominent absorption bands in the range of 400 to 600 cm-1 which are the fingerprint region of all ferrites...
2017: PloS One
https://www.readbyqxmd.com/read/28079221/sizing-and-eddy-currents-in-magnetic-core-nanoparticles-an-optical-extinction-approach
#3
Luis J Mendoza Herrera, Ignacio J Bruvera, Lucía B Scaffardi, Daniel C Schinca
Optical extinction is a handy and ubiquitous technique that allows us to study colloidal nanoparticles in their native state. The typical analysis of the extinction spectrum can be extended in order to obtain structural information of the sample such as the size distribution of the cores and the thickness of the coating layers. In this work the extinction spectra of Fe3O4, Fe3O4@Au, and Fe3O4@SiO2@Au single and multilayer nanoparticles are obtained by solving full Mie theory with a frequency dependent susceptibility derived from the Gilbert equation and considering the effect of Eddy currents...
January 12, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28078862/a-placebo-controlled-trial-of-a-proprietary-lipid-lowering-nutraceutical-supplement-in-the-management-of-dyslipidemia
#4
M Houston, R Rountree, J Lamb, S Phipps, S Meng, B Zhang
There is an ever growing emergence in the popularity of patient-driven care. As this health and wellness model grows, inquiries into diet, lifestyle, and supplemental approaches will continue to become a focal point for the healthcare consumer. Because of this, the aim of this study is to determine the tolerability, and overall effectiveness of a proprietary multi-ingredient lipid-lowering supplement in subjects with dyslipidemia. Forty participants were recruited for a single-center, double-blind randomized, placebocontrolled trial...
October 2016: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/28077236/high-resolution-ultrahigh-pressure-long-column-reversed-phase-liquid-chromatography-for-top-down-proteomics
#5
Yufeng Shen, Nikola Tolić, Paul D Piehowski, Anil K Shukla, Sangtae Kim, Rui Zhao, Yi Qu, Errol Robinson, Richard D Smith, Ljiljana Paša-Tolić
Separation of proteoforms for global intact protein analysis (i.e. top-down proteomics) has lagged well behind what is achievable for peptides in traditional bottom-up proteomic approach and is becoming a true bottle neck for top-down proteomics. Herein, we report use of long (≥1M) columns containing short alkyl (C1-C4) bonded phases to achieve high-resolution RPLC for separation of proteoforms. At a specific operation pressure limit (i.e., 96.5MPa or 14Kpsi used in this work), column length was found to be the most important factor for achieving maximal resolution separation of proteins when 1...
January 5, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28076019/viral-and-prion-infections-of-the-central-nervous-system-radiologic-pathologic-correlation-from-the-radiologic-pathology-archives
#6
Kelly K Koeller, Robert Y Shih
Viral infections of the central nervous system (CNS) range in clinical severity, with the most severe proving fatal within a matter of days. Some of the more than 100 different viruses known to affect the brain and spinal cord are neurotropic with a predilection for producing CNS infection. The host response to viral infection of the CNS is responsible for the pathophysiology and imaging findings seen in affected patients. Viral CNS infections can take the form of meningitis, encephalitis, encephalomyelitis, or, when involving the spinal cord and nerve roots, encephalomyeloradiculitis...
January 2017: Radiographics: a Review Publication of the Radiological Society of North America, Inc
https://www.readbyqxmd.com/read/28072489/development-and-application-of-sub-2%C3%A2-%C3%A2%C2%B5m-particle-co2-based-chromatography-coupled-to-mass-spectrometry-for-comprehensive-analysis-of-lipids-in-cottonseed-extracts
#7
Carolina Salazar, Michael D Jones, Drew Sturtevant, Patrick J Horn, Janna Crossley, Khadiza Zaman, Kent D Chapman, Mark Wrona, Giorgis Isaac, Norman Smith, Vladimir Shulaev
RATIONALE: Refined cottonseed oil has widespread applications in the food and chemical industries. Although the major lipids comprising cottonseed oil (triacylglycerols) are well known, there are many diverse lipid species in cotton seeds that occur at much lower levels and have important nutritional or anti-nutritional properties. METHODS: The lipid technical samples were prepared in chloroform. The biological samples were extracted using a mixture of isopropanol:chloroform:H2 O (2:1:0...
January 10, 2017: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/28070848/intravaginal-delivery-of-polyphenon-60-and-curcumin-nanoemulsion-gel
#8
Atinderpal Kaur, Yashaswee Saxena, Rakhi Bansal, Sonal Gupta, Amit Tyagi, Rakesh Kumar Sharma, Javed Ali, Amulya Kumar Panda, Reema Gabrani, Shweta Dang
Polyphenon 60 (P60) and curcumin (CUR) were loaded in a single nanoemulsion system and their combined antibacterial action was studied against uropathogenic Escherichia coli. To enhance availability at target organs and to inhibit enzymatic degradation in gastro intestinal tract, vaginal route of administration was explored. P60 + CUR nanoemulsion (NE) was formulated by ultra-sonication and optimized using Box-Behnken design. Optimized NE showed Z-average of 211.2 nm, polydispersity index of 0.343, and zeta potential of -32...
January 9, 2017: AAPS PharmSciTech
https://www.readbyqxmd.com/read/28069206/optimization-of-simultaneous-ultrasound-assisted-toxic-dyes-adsorption-conditions-from-single-and-multi-components-using-central-composite-design-application-of-derivative-spectrophotometry-and-evaluation-of-the-kinetics-and-isotherms
#9
Ebrahim Sharifpour, Hedayat Haddadi, Mehrorang Ghaedi
Present study is devoted on the efficient application of Sn (O, S)-NPs -AC for simultaneous sonicated accelerated adsorption of some dyes from single and multi-components systems. Sn (O, S) nanoparticles characterization by FESEM, EDX, EDX mapping and XRD revel its nano size structure with high purity of good crystallinity. Present adsorbent due to its nano spherical shape particles with approximate diameter of 40-60nm seems to be highly effective in this regard. The effects of five variables viz. pH (3.5-9...
May 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28065477/quantitative-analysis-of-the-yield-of-avian-h7-influenza-virus-haemagglutinin-protein-produced-in-silkworm-pupae-with-the-use-of-the-codon-optimized-dna-a-possible-oral-vaccine
#10
Kuniaki Nerome, Sayaka Matsuda, Kenichi Maegawa, Shigeo Sugita, Kazumichi Kuroda, Kazunori Kawasaki, Reiko Nerome
In this study, we aimed to quantitatively compare the increased production of three H7 influenza virus-like particle (VLP) haemagglutinin (HA) with the use of a codon-optimized single HA gene in silkworm pupae. Recombinant baculovirus (Korea H7-BmNPV) could produce 0.40 million HA units per pupa, corresponding to 1832μg protein. The yield of the HA produced in larva was estimated to be approximately 0.31 million HA units per larva, and there were no significant differences between the three HA proteins. We could establish efficient recovery system of HA production in larvae and pupae with the use of three cycles sonication methods...
January 5, 2017: Vaccine
https://www.readbyqxmd.com/read/28064392/chemical-composition-structural-properties-and-source-apportionment-of-organic-macromolecules-in-atmospheric-pm10-in-a-coastal-city-of-southeast-china
#11
Yanting Chen, Wenjiao Du, Jinsheng Chen, Youwei Hong, Jinping Zhao, Lingling Xu, Hang Xiao
Particulate matter (PM10) associated with the fractions of organic macromolecules, including humic acid (HA), kerogen + black carbon (KB), and black carbon (BC), was determined during summer and winter at urban and suburban sites in a coastal city of southeast China. The organic macromolecules were characterized by elemental analysis (EA), scanning electron microscopy (SEM), and Fourier transform infrared spectroscopy (FTIR), and their sources were identified by using stable carbon/nitrogen isotope (δ(13)C/δ(15)N) and the Hybrid Single Particle Lagrangian Integrated Trajectory (HYSPLIT) Model...
January 7, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28063711/fine-particulates-over-south-asia-review-and-meta-analysis-of-pm2-5-source-apportionment-through-receptor-model
#12
Nandita Singh, Vishnu Murari, Manish Kumar, S C Barman, Tirthankar Banerjee
Fine particulates (PM2.5) constitute dominant proportion of airborne particulates and have been often associated with human health disorders, changes in regional climate, hydrological cycle and more recently to food security. Intrinsic properties of particulates are direct function of sources. This initiates the necessity of conducting a comprehensive review on PM2.5 sources over South Asia which in turn may be valuable to develop strategies for emission control. Particulate source apportionment (SA) through receptor models is one of the existing tool to quantify contribution of particulate sources...
January 4, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28061390/wettability-and-surface-morphology-of-eroded-dentin-treated-with-chitosan
#13
Mirian Saavedra Ururahy, Fabiana Almeida Curylofo-Zotti, Rodrigo Galo, Lucas Fabricio Bahia Nogueira, Ana Paula Ramos, Silmara Aparecida Milori Corona
OBJECTIVE: To assess the effect of chitosan, at concentrations of 2.5% and 5.0%, on the wettability of the eroded dentin, followed by analysis of surface morphology by SEM. METHODS: 104 bovine dentin slabs were ground, polished and then immersed in 20mL of citric acid (pH=3.2) under continuous stirring for 2h. Specimens were randomly divided according to the dentin substrate: sound and eroded, and then, subdivided into 4 groups (n=10): without rewetting (control), 1% acetic acid, 2...
December 2, 2016: Archives of Oral Biology
https://www.readbyqxmd.com/read/28057332/influence-of-geometry-and-material-of-insulating-posts-on-particle-trapping-using-positive-dielectrophoresis
#14
Georg R Pesch, Fei Du, Michael Baune, Jorg Thöming
Insulator-based dielectrophoresis (iDEP) is a powerful particle analysis technique based on electric field scattering at material boundaries which can be used, for example, for particle filtration or to achieve chromatographic separation. Typical devices consist of microchannels containing an array of posts but large scale application was also successfully tested. Distribution and magnitude of the generated field gradients and thus the possibility to trap particles depends apart from the applied field strength on the material combination between post and surrounding medium and on the boundary shape...
December 26, 2016: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28045263/single-and-double-strand-breaks-of-dry-dna-exposed-to-protons-at-the-bragg-peak-energies
#15
Mounir Souici, Talat Tariq Khalil, Dominique Muller, Quentin Raffy, Rémi Barillon, Abdelfettah Belafrites, Christophe Champion, Michel Fromm
Ultra-thin layers (<20 nm) of pBR322 plasmid DNA were deposited onto 2.5 µm thick polyester films and exposed to proton Bragg-peak energies (90-3000 keV) at various fluences. A quantitative analysis of the DNA damage radio-induction is here reported in terms of single and double strand breaks (SSB and DSB, respectively). The corresponding yields as well as G-values and the cross sections exhibit a fairly good agreement with the rare available data stemming from close experimental conditions; namely based on alpha particles irradiations...
January 3, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28044291/droplet-microfluidic-and-magnetic-particles-platform-for-cancer-typing
#16
Davide Ferraro, Jérôme Champ, Bruno Teste, M Serra, Laurent Malaquin, Stéphanie Descroix, Patricia de Cremoux, Jean-Louis Viovy
Analyses of nucleic acids are routinely performed in hospital laboratories to detect gene alterations for cancer diagnosis and treatment decision. Among the different possible investigations, mRNA analysis provides information on abnormal levels of genes expression. Standard laboratory methods are still not adapted to the isolation and quantitation of low mRNA amounts and new techniques needs to be developed in particular for rare subsets analysis. By reducing the volume involved, time process, and the contamination risks, droplet microfluidics provide numerous advantages to perform analysis down to the single cell level...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28039566/the-structure-of-fcpb-a-light-harvesting-complex-in-the-diatom-cyclotella-meneghiniana
#17
Anja Röding, Egbert Boekema, Claudia Büchel
Diatoms possess fucoxanthin chlorophyll proteins (FCP) as light-harvesting systems. These membrane intrinsic proteins bind fucoxanthin as major carotenoid and Chl c as accessory chlorophyll. The relatively high sequence homology to higher plant light-harvesting complex II gave rise to the assumption of a similar overall structure. From centric diatoms like Cyclotella meneghiniana, however, two major FCP complexes can be isolated. FCPa, composed of Fcp2 and Fcp6 subunits, was demonstrated to be trimeric, whereas FCPb, known to contain Fcp5 polypeptides, is of higher oligomeric state...
December 30, 2016: Photosynthesis Research
https://www.readbyqxmd.com/read/28035048/live-cell-single-molecule-labeling-and-analysis-of-myosin-motors-with-quantum-dots
#18
Hiroyasu Hatakeyama, Yoshihito Nakahata, Hirokazu Yarimizu, Makoto Kanzaki
Quantum dots (QDs) are a powerful tool for quantitatively analyzing dynamic cellular processes by single-particle tracking. However, tracking of intracellular molecules with QDs is limited by their inability to penetrate the plasma membrane and bind to specific molecules of interest. Although several techniques for overcoming these problems have been proposed, they are either complicated or inconvenient. To address this issue, in this study, we developed a simple, convenient, and nontoxic method for labeling intracellular molecules in cells using HaloTag technology and electroporation...
January 1, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28034735/hydrophilic-drug-encapsulation-in-shell-core-microcarriers-by-two-stage-polyelectrolyte-complexation-method
#19
Annalisa Dalmoro, Alexander Y Sitenkov, Sara Cascone, Gaetano Lamberti, Anna Angela Barba, Rouslan I Moustafine
In this study a protocol exploiting the combination of the ultrasonic atomization and the complexation between polyelectrolytes was developed to efficiently encapsulate a hydrophilic chemotherapeutic agent essentially used in the treatment of colon cancer, 5-fluorouracil, in enteric shell-core alginate-based microcarriers. The atomization assisted by ultrasound allowed to obtain small droplets by supplying low energy and avoiding drug degradation. In particular microcarriers were produced in a home-made apparatus where both the core (composed of alginate, drug, and Pluronic F127) and shell (composed of only alginate) feed were separately sent to the coaxial ultrasonic atomizer where they were nebulized and placed in contact with the complexation bulk...
December 26, 2016: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28033028/single-particle-analysis-methods-for-detection-of-platelet-extracellular-vesicles-in-suspension-excluding-flow-cytometry
#20
Edit I Buzás, Chris Gardiner, Changwon Lee, Zachary J Smith
Extracellular vesicles (EVs) are small, membrane-bound particles released by all cell types, including abundant release by platelets. EVs are a topic of increasing interest in the academic and clinical community due to their increasingly recognised and diverse role in normal biology as well as in disease. However, typical analysis methods to characterise EVs released by cultured cells or isolated from whole blood or other body fluids are restricted to bulk analysis of all EVs in a sample. In this review, we discuss the motivation for analysis of individual EVs, as well as discuss three emerging methods for physical and chemical characterisation of individual EVs: nanoparticle tracking analysis, tunable resistive pulse sensing and Raman spectroscopy...
December 29, 2016: Platelets
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