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single particle analysis

Yusuke Matsui, Masahiro Horikawa, Younes Jahangiri Noudeh, John A Kaufman, Kenneth J Kolbeck, Khashayar Farsad
Background: The aim of the study was to evaluate the association between baseline Lipiodol uptake in hepatocellular carcinoma (HCC) after transarterial chemoembolization (TACE) with early tumor recurrence, and to identify a threshold baseline uptake value predicting tumor response. Patients and methods: A single-institution retrospective database of HCC treated with Lipiodol-TACE was reviewed. Forty-six tumors in 30 patients treated with a Lipiodol-chemotherapy emulsion and no additional particle embolization were included...
December 2017: Radiology and Oncology
S Candás-Zapico, D J Kutscher, M Montes-Bayón, J Bettmer
Titanium dioxide (TiO2) belongs to the materials that have gained great importance in many applications. In its particulate form (micro- or nanoparticles), it has entered a huge number of consumer products and food-grade TiO2, better known as E171 within the European Union, represents an important food additive. Thus, there is an increasing need for analytical methods able to detect and quantify such particles. In this regard, inductively coupled-mass spectrometry (ICP-MS), in particular single particle ICP-MS (spICP-MS), has gained importance due to its simplicity and ease of use...
April 1, 2018: Talanta
Zahra Hussaini, Pin Ann Lin, Bharath Natarajan, Wenhui Zhu, Renu Sharma
For many reaction processes, such as catalysis, phase transformations, nanomaterial synthesis etc., nanoscale observations at high spatial (sub-nanometer) and temporal (millisecond) resolution are required to characterize and comprehend the underlying factors that favor one reaction over another. The combination of such spatial and temporal resolution (up to 600 µs), while rich in information, produces a large number of snapshots, each of which must be analyzed to obtain the structural (and thereby chemical) information...
December 28, 2017: Ultramicroscopy
S Kumbhani, T Longin, L M Wingen, C Kidd, V Perraud, B J Finlayson-Pitts
Real-time in situ mass spectrometry analysis of airborne particles is important in several applications, including exposure studies in ambient air, industrial settings, and assessing impacts on visibility and climate. However, obtaining molecular and 3D structural information is more challenging, especially for heterogeneous solid or semisolid particles. We report a study of extractive electrospray ionization mass spectrometry (EESI-MS) for the analysis of solid particles with an organic coating. The goal is to elucidate how much of the overall particle content is sampled, and determine the sensitivity of this technique to the surface layers...
January 11, 2018: Analytical Chemistry
Xuexiao Cao, Meng Wang, Lili Sun, Xiaoliang Ren, Gaosheng Pei
BACKGROUND: The highly selective capture of individual components of complex systems such as Chinese medicine extracts is a great challenge. With the rapid development of nanoscience, magnetic materials have attracted increased attention as suitable candidates for use in drug transport vehicles. RESULTS: In this work, amino-modified Fe3 O4 nanoparticles (MNP-NH2 ) have been synthesized and successfully used to selectively isolate Luteolin, a type of flavonoid, from peanut shell, coupled with high-performance liquid chromatography (HPLC)...
January 9, 2018: Journal of the Science of Food and Agriculture
Yoshihiko Hagiwara, Atsuko Niimi, Mayu Isono, Motohiro Yamauchi, Takaaki Yasuhara, Siripan Limsirichaikul, Takahiro Oike, Hiro Sato, Kathryn D Held, Takashi Nakano, Atsushi Shibata
DNA double-strand breaks (DSBs) induced by ionising radiation are considered the major cause of genotoxic mutations and cell death. While DSBs are dispersed throughout chromatin after X-rays or γ-irradiation, multiple types of DNA damage including DSBs, single-strand breaks and base damage can be generated within 1-2 helical DNA turns, defined as a complex DNA lesion, after high Linear Energy Transfer (LET) particle irradiation. In addition to the formation of complex DNA lesions, recent evidence suggests that multiple DSBs can be closely generated along the tracks of high LET particle irradiation...
December 12, 2017: Oncotarget
Douglas R Keene, Sara F Tufa
This chapter gives insight into task-specific methodologies for the evaluation of matrix ultrastructure by light and electron microscopy. It separately considers the isolation and preparation of molecular isolates for negative staining, immunolabeling, rotary shadowing, and single particle analysis. Also considered is the preparation of whole tissues and cultured cells by chemical fixation and cryofixation methodologies. Immunoelectron microscopy for immunoidentification of matrix components may be accomplished en bloc or via section-surface protocols; the advantages and pitfalls in both methodologies are described...
2018: Methods in Cell Biology
Miroslava Beňovská, Ondřej Wiewiorka, Jana Pinkavová
This study evaluates the performance of microscopic part of a hybrid analyzer FUS-2000 (Dirui Industrial Co., Changchun, China), its analytical properties and particle recognition. The evaluation of trueness, repeatability, detection limit, carry-over, linearity range and analytical stability was performed according to Dirui protocol guidelines designed by Dirui Company to guarantee the quality of the instrument. Trueness for low, medium and high-value concentrations was calculated with bias of 15.5, 4.7 and -6...
January 8, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
O A Goryacheva, P K Mishra, I Yu Goryacheva
MicroRNAs (miRNAs) are a class of small non-coding RNAs that are involved in nearly all developmental processes and human pathologies. MiRNAs are considered to be promising biomarkers, since their dysregulation correlates with the development and progress of many diseases. Short length, sequence homology among family members, susceptibility to degradation, and low abundance in total RNA samples make miRNA analysis a challenging task. Photoluminescent semiconductor nanoparticles (quantum dots, QDs) possess unique properties such as bright photoluminescence, photostability and narrow emission peaks, wide possibilities for surface modification and bioconjugation, which serve as the basis for the development of different analytical methods for variety of analytes...
March 1, 2018: Talanta
Albert Kéri, Ildikó Kálomista, Ditta Ungor, Ádám Bélteki, Edit Csapó, Imre Dékány, Thomas Prohaska, Gábor Galbács
In this study, the information that can be obtained by combining normal and high resolution single particle ICP-MS (spICP-MS) measurements for spherical bimetallic nanoparticles (BNPs) was assessed. One commercial certified core-shell Au-Ag nanoparticle and three newly synthesized and fully characterized homogenous alloy Au-Ag nanoparticle batches of different composition were used in the experiments as BNP samples. By scrutinizing the high resolution spICP-MS signal time profiles, it was revealed that the width of the signal peak linearly correlates with the diameter of nanoparticles...
March 1, 2018: Talanta
Julian Kappler, Jan O Daldrop, Florian N Brünig, Moritz D Boehle, Roland R Netz
We study the mean first-passage time τMFP for the barrier crossing of a single massive particle with non-Markovian memory by Langevin simulations in one dimension. In the Markovian limit of short memory time τΓ, the expected Kramers turnover between the overdamped (high-friction) and the inertial (low-friction) limits is recovered. Compared to the Markovian case, we find barrier crossing to be accelerated for intermediate memory time, while for long memory time, barrier crossing is slowed down and τMFP increases with τΓ as a power law τMFP∼τΓ2...
January 7, 2018: Journal of Chemical Physics
Muntazim Munir Khan, Sergey Shishatskiy, Volkan Filiz
This work reports on the preparation and gas transport performance of mixed matrix membranes (MMMs) based on the polymer of intrinsic microporosity (PIM-1) and potassium dodecahydrododecaborate (K₂B12H12) as inorganic particles (IPs). The effect of IP loading on the gas separation performance of these MMMs was investigated by varying the IP content (2.5, 5, 10 and 20 wt %) in a PIM-1 polymer matrix. The derived MMMs were characterized by scanning electron microscopy (SEM), thermogravimetric analysis (TGA), single gas permeation tests and sorption measurement...
January 2, 2018: Membranes
Lucas M Oliveira, Ze Ye, Al Katz, Alexandra Alimova, Hui Wei, Gabor T Herman, Paul Gottlieb
The 3-dimensional structure of the nucleocapsid (NC) of bacteriophage φ6 is described utilizing component tree analysis, a topological and geometric image descriptor. The component trees are derived from density maps of cryo-electron microscopy single particle reconstructions. Analysis determines position and occupancy of structure elements responsible for RNA packaging and transcription. Occupancy of the hexameric nucleotide triphosphorylase (P4) and RNA polymerase (P2) are found to be essentially complete in the NC...
2018: PloS One
Ye Tian, Ling Ma, Manfei Gong, Guoqiang Su, Shaobin Zhu, Wenqiang Zhang, Shuo Wang, Zhibin Li, Chaoxiang Chen, Lihong Li, Lina Wu, Xiaomei Yan
Extracellular vesicles (EVs) have stimulated considerable scientific and clinical interest, yet protein profiling and sizing of individual EVs remains challenging due to their small particle size, low abundance of proteins, and overall heterogeneity. Building upon a laboratory-built high-sensitivity flow cytometer (HSFCM), we report here a rapid approach for quantitative multiparameter analysis of single EVs down to 40 nm with an analysis rate up to 10,000 particles per minute. Statistically robust particle size distribution was acquired in minutes with a resolution and profile well matched with those of cryo-TEM measurements...
January 4, 2018: ACS Nano
Anders S Hansen, Maxime Woringer, Jonathan B Grimm, Luke D Lavis, Robert Tjian, Xavier Darzacq
Single-particle tracking (SPT) has become an important method to bridge biochemistry and cell biology since it allows direct observation of protein binding and diffusion dynamics in live cells. However, accurately inferring information from SPT studies is challenging due to biases in both data analysis and experimental design. To address analysis bias, we introduce 'Spot-On', an intuitive web-interface. Spot-On implements a kinetic modeling framework that accounts for known biases, including molecules moving out-of-focus, and robustly infers diffusion constants and subpopulations from pooled single-molecule trajectories...
January 4, 2018: ELife
Netanel Shpigel, Mikhael D Levi, Sergey Sigalov, Leonid Daikhin, Doron Aurbach
Quartz crystal microbalance with dissipation monitoring (QCM-D) generates surface-acoustic waves in quartz crystal plates that can effectively probe the structure of films, particulate composite electrodes of complex geometry rigidly attached to quartz crystal surface on one side and contacting a gas or liquid phase on the other side. The output QCM-D characteristics consist of the resonance frequency (MHz frequency range) and resonance bandwidth measured with extra-ordinary precision of a few tenths of Hz...
January 3, 2018: Accounts of Chemical Research
Yan Zhang, Jun Zhang, Fei Tang, Weihua Li, Xiaohao Wang
High-throughput, high-precision single-stream focusing of microparticles has potential wide range of applications in bio-chemical analysis and clinical diagnosis. In this work, we develop a sheathless three-dimensional (3D) particle focusing method in a single-layer microchannel. This novel microchannel consists of periodic high-aspect-ratio curved channels and straight channels. The proposed method takes advantage of both the curved channels, which induce Dean flow to promote particle migration, and straight channels, which suppress the remaining stirring effects of Dean flow to stabilize the achieved particle focusing...
January 3, 2018: Analytical Chemistry
Fredmoore L Orosco, Arturo O Lluisma
Marine animals typically harbor a community of viruses, a number of which are known to cause diseases. In shrimp aquaculture, viral pathogens are the principal causes of major economic losses. However, the composition of the viral load of shrimps in wild population is poorly known. In this study, we explored the viral diversity in the microbiome of wild Penaeus monodon collected from six sites in the Philippines, with a view to detecting pathogenic forms. We employed a metagenomic approach via particle-associated nucleic acid isolation, sequence-independent single primer amplification, and pyrosequencing...
September 2017: Virusdisease
Kevin Cushing, Eva Undvall, Yvonne Ceder, Hans Lilja, Thomas Laurell
Cancer cells display acoustic properties enabling acoustophoretic separation from white blood cells (WBCs) with 2-3 log suppression of the WBC background. However, a subset of WBCs has overlapping acoustic properties with cancer cells, which is why label-free acoustophoretic cancer cell isolation needs additional purification prior to analysis. This paper reports for the first time a proof of concept for continuous flow acoustophoretic negative selection of WBCs from cancer cells using negative acoustic contrast elastomeric particles (EPs) activated with CD45-antibodies that specifically bind to WBCs...
February 13, 2018: Analytica Chimica Acta
S Huang, B Mohanty, K Xia
Rock crushing is a common process in mining and related operations. Although a number of particle crushing tests have been proposed in the literature, most of them are concerned with single-particle crushing, i.e., a single rock sample is crushed in each test. Considering the realistic scenario in crushers where many fragments are involved, a laboratory crushing apparatus is developed in this study. This device consists of a Hopkinson pressure bar system and a piston-holder system. The Hopkinson pressure bar system is used to apply calibrated dynamic loads to the piston-holder system, and the piston-holder system is used to hold rock samples and to recover fragments for subsequent particle size analysis...
December 2017: Review of Scientific Instruments
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