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https://www.readbyqxmd.com/read/28212518/zno-tio2-composites-for-photocatalytic-inactivation-of-escherichia-coli
#1
Diptipriya Sethi, Ramasamy Sakthivel
ZnO/TiO2 composite was synthesized from zinc nitrate and anatase TiO2 by using the incipient wet impregnation method followed by calcination at 350°C for 3h. These samples were characterized through several characterization techniques. XRD peaks confirms the presence of single anatase phase of titania in the 3 and 6wt% ZnO/TiO2 composite, whereas in all other composites both anatase titania and zinc oxide phases were found. UV-visible (diffuse) reflectance spectra show titania and ZnO absorbs in the ultraviolet (UV) region whereas ZnO/TiO2 absorbs both UV and visible light...
February 11, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28210618/an-optimized-procedure-for-the-site-directed-labeling-of-ngf-and-prongf-for-imaging-purposes
#2
Pierluigi Di Matteo, Mariantonietta Calvello, Stefano Luin, Laura Marchetti, Antonino Cattaneo
Neurotrophins are growth factors of fundamental importance for the development, survival and maintenance of different neuronal and non-neuronal populations. Over the years, the use of labeled neurotrophins has helped in the study of their biological functions, leading to a better understanding of the processes that regulate their transport, traffic, and signaling. However, the diverse and heterogeneous neurotrophin labeling strategies adopted so far have often led to poorly reproducible protocols and sometimes conflicting conclusions...
2017: Frontiers in Molecular Biosciences
https://www.readbyqxmd.com/read/28209364/variable-threshold-method-for-determining-the-boundaries-of-imaged-subvisible-particles
#3
R E Cavicchi, Cayla Collett, Srivalli Telikepalli, Zhishang Hu, Michael Carrier, Dean C Ripple
An accurate assessment of particle characteristics and concentrations in pharmaceutical products by flow imaging requires accurate particle sizing and morphological analysis. Analysis of images begins with the definition of particle boundaries. Commonly a single threshold defines the level for a pixel in the image to be included in the detection of particles, but depending on the threshold level, this results in either missing translucent particles or oversizing of less transparent particles due to the halos and gradients in intensity near the particle boundaries...
February 13, 2017: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28208767/alternative-cdep-design-to-facilitate-cell-isolation-for-identification-by-raman-spectroscopy
#4
Cynthia Hanson, Elizabeth Vargis
Dielectrophoresis (DEP) uses non-uniform electric fields to cause motion in particles due to the particles' intrinsic properties. As such, DEP is a well-suited label-free means for cell sorting. Of the various methods of implementing DEP, contactless dielectrophoresis (cDEP) is advantageous as it avoids common problems associated with DEP, such as electrode fouling and electrolysis. Unfortunately, cDEP devices can be difficult to fabricate, replicate, and reuse. In addition, the operating parameters are limited by the dielectric breakdown of polydimethylsiloxane (PDMS)...
February 9, 2017: Sensors
https://www.readbyqxmd.com/read/28208588/depicting-binding-mediated-translocation-of-hiv-1-tat-peptides-in-living-cells-with-nanoscale-pens-of-tat-conjugated-quantum-dots
#5
Chien Y Lin, Jung Y Huang, Leu-Wei Lo
Cell-penetrating peptides (CPPs) can translocate across cell membranes, and thus have great potential for the cellular delivery of macromolecular cargoes. However, the mechanism of this cellular uptake process is not yet fully understood. In this study, a time-lapse single-particle light-sheet microscopy technique was implemented to obtain a parallel visualization of the translocating process of individual human immunodeficiency virus 1 (HIV-1) transactivator of transcription (Tat) peptide conjugated quantum dots (TatP-QDs) in complex cellular terrains...
February 10, 2017: Sensors
https://www.readbyqxmd.com/read/28208346/density-of-states-from-mode-expansion-of-the-self-dynamic-structure-factor-of-a-liquid-metal
#6
E Guarini, S Bellissima, U Bafile, E Farhi, A De Francesco, F Formisano, F Barocchi
We show that by exploiting multi-Lorentzian fits of the self-dynamic structure factor at various wave vectors it is possible to carefully perform the Q→0 extrapolation required to determine the spectrum Z(ω) of the velocity autocorrelation function of a liquid. The smooth Q dependence of the fit parameters makes their extrapolation to Q=0 a simple procedure from which Z(ω) becomes computable, with the great advantage of solving the problems related to resolution broadening of either experimental or simulated self-spectra...
January 2017: Physical Review. E
https://www.readbyqxmd.com/read/28208315/tracer-counterpermeation-analysis-of-diffusivity-in-finite-length-nanopores-with-and-without-single-file-dynamics
#7
David M Ackerman, James W Evans
We perform a tracer counterpermeation (TCP) analysis for a stochastic model of diffusive transport through a narrow linear pore where passing of species within the pore is inhibited or even excluded (single-file diffusion). TCP involves differently labeled but otherwise identical particles from two decoupled infinite reservoirs adsorbing into opposite ends of the pore, and desorbing from either end. In addition to transient behavior, we assess steady-state concentration profiles, spatial correlations, particle number fluctuations, and diffusion fluxes through the pore...
January 2017: Physical Review. E
https://www.readbyqxmd.com/read/28207240/interfaces-in-heterogeneous-catalysts-advancing-mechanistic-understanding-through-atomic-scale-measurements
#8
Wenpei Gao, Zachary D Hood, Miaofang Chi
Developing novel catalysts with high efficiency and selectivity is critical for enabling future clean energy conversion technologies. Interfaces in catalyst systems have long been considered the most critical factor in controlling catalytic reaction mechanisms. Interfaces include not only the catalyst surface but also interfaces within catalyst particles and those formed by constructing heterogeneous catalysts. The atomic and electronic structures of catalytic surfaces govern the kinetics of binding and release of reactant molecules from surface atoms...
February 16, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/28206760/nano-engineered-eggshell-silver-tailored-co-polyester-polymer-blend-film-with-antimicrobial-properties
#9
Boniface J Tiimob, Gregory Mwinyelle, Woubit Abdela, Temesgen Samuel, Shaik Jeelani, Vijaya K Rangari
In this study, the reinforcement effect of different proportions of eggshell/silver nanomaterial (ES-Ag) on structural and antimicrobial properties of 70/30 poly (butylene-co-adipate terephthalate)/polylactic acid (PBAT/PLA) immiscible blends were investigated. The ES-Ag was synthesized using a single step ball milling process, and characterized with X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) and transmission electron microscopy (TEM). These results confirmed the existence of silver nanoparticles (Ag NPs) in the interstitial spaces of the eggshell particles...
February 16, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28199086/crystal-growth-and-structure-analysis-of-ce18w10o57-a-complex-oxide-containing-tungsten-in-an-unusual-trigonal-prismatic-coordination-environment
#10
Dileka Abeysinghe, Mark D Smith, Jeongho Yeon, T Thao Tran, Robert Paria Sena, Joke Hadermann, P Shiv Halasyamani, Hans-Conrad Zur Loye
The noncentrosymmetric tungstate oxide, Ce18W10O57, was synthesized for the first time as high-quality single crystals via the molten chloride flux method and structurally characterized by single-crystal X-ray diffraction. The compound is a structural analogue to the previously reported La18W10O57, which crystallizes in the hexagonal space group P6̅2c. The +3 oxidation state of cerium in Ce18W10O57 was achieved via the in situ reduction of Ce(IV) to Ce(III) using Zn metal. The structure consists of both isolated and face-shared WO6 octahedra and, surprisingly, isolated WO6 trigonal prisms...
February 15, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28198180/engineering-recombinant-virus-like-nanoparticles-from-plants-for-cellular-delivery
#11
Lou Brillault, Philippe V Jutras, Noor Dashti, Eva C Thuenemann, Garry Morgan, George P Lomonossoff, Michael J Landsberg, Frank Sainsbury
Understanding capsid assembly following recombinant expression of viral structural proteins is critical to the design and modification of virus-like nanoparticles for biomedical and nanotechnology applications. Here, we use plant-based transient expression of the Bluetongue virus (BTV) structural proteins, VP3 and VP7, to obtain high yields of empty and green fluorescent protein (GFP)-encapsidating core-like particles (CLPs) from leaves. Single-particle cryo-electron microscopy of both types of particles revealed considerable differences in CLP structure compared to the crystal structure of infection-derived CLPs; in contrast, the two recombinant CLPs have an identical external structure...
February 15, 2017: ACS Nano
https://www.readbyqxmd.com/read/28196962/spatial-and-temporal-analysis-of-alphavirus-replication-and-assembly-in-mammalian-and-mosquito-cells
#12
Joyce Jose, Aaron B Taylor, Richard J Kuhn
Sindbis virus (SINV [genus Alphavirus, family Togaviridae]) is an enveloped, mosquito-borne virus. Alphaviruses cause cytolytic infections in mammalian cells while establishing noncytopathic, persistent infections in mosquito cells. Mosquito vector adaptation of alphaviruses is a major factor in the transmission of epidemic strains of alphaviruses. Though extensive studies have been performed on infected mammalian cells, the morphological and structural elements of alphavirus replication and assembly remain poorly understood in mosquito cells...
February 14, 2017: MBio
https://www.readbyqxmd.com/read/28195734/bovine-serum-albumin-adsorption-on-tio2-colloids-the-effect-of-particle-agglomeration-and-surface-composition
#13
Augusto Márquez, Thomas Berger, Andrea Feinle, Nicola Huesing, Martin Himly, Albert Duschl, Oliver Diwald
Protein adsorption at nanostructured oxides strongly depends on synthesis conditions and sample history of the material investigated. We measured the adsorption of bovine serum albumin (BSA) to commercial Aeroxide TiO2 P25 nanoparticles in aqueous dispersions. Significant changes in adsorption capacity were induced by mild sample washing procedures and attributed to the structural modification of adsorbed water and surface hydroxyls. Motivated by the lack of information about the sample history of commercial TiO2 nanoparticle samples we used vapor phase grown TiO2 nanoparticles, a well-established model system for adsorption and photo-catalysis studies, and performed on this material for the first time a systematic and quantitative BSA adsorption study...
February 14, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28195202/application-of-pristine-and-doped-sno2-nanoparticles-as-a-matrix-for-agro-hazardous-material-organophosphate-detection
#14
Naushad Khan, Taimur Athar, H Fouad, Ahmad Umar, Z A Ansari, S G Ansari
With an increasing focus on applied research, series of single/composite materials are being investigated for device development to detect several hazardous, dangerous, and toxic molecules. Here, we report a preliminary attempt of an electrochemical sensor fabricated using pristine Ni and Cr-doped nano tin oxide material (SnO2) as a tool to detect agro-hazardous material, i.e. Organophosphate (OP, chlorpyrifos). The nanomaterial was synthesized using the solution method. Nickel and chromium were used as dopant during synthesis...
February 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28194674/biomonitoring-of-atmospheric-pollution-a-novel-approach-for-the-evaluation-of-natural-and-anthropogenic-contribution-to-atmospheric-aerosol-particles
#15
Rosa Caggiano, Giuseppe Calamita, Serena Sabia, Serena Trippetta
The investigation of the potential natural and anthropogenic contribution to atmospheric aerosol particles by using lichen-bag technique was performed in the Agri Valley (Basilicata region, southern Italy). This is an area of international concern since it houses one of the largest European on-shore reservoirs and the biggest oil/gas pre-treatment plant (i.e., Centro Olio Val d'Agri (COVA)) within an anthropized context. In particular, the concentrations of 17 trace elements (Al, Ca, Cd, Cr, Cu, Fe, K, Li, Mg, Mn, Na, Ni, P, Pb, S, Ti, and Zn) were measured in lichen bags exposed in 59 selected monitoring points over periods of 6 months (from October 2011 to April 2012) and 12 months (from October 2011 to October 2012)...
February 13, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28193500/helical-reconstruction-in-relion
#16
Shaoda He, Sjors H W Scheres
We describe a new implementation for the reconstruction of helical assemblies in the empirical Bayesian framework of RELION. Our approach calculates optimal linear filters for the 3D reconstruction by embedding helical symmetry operators in Fourier-space, and deals with deviations from perfect helical symmetry through Gaussian-shaped priors on the orientations of individual segments. By incorporating our approach into the standard pipeline for single-particle analysis in RELION, our implementation aims to be easily accessible for non-experienced users...
February 10, 2017: Journal of Structural Biology
https://www.readbyqxmd.com/read/28192943/ultrafast-chiral-chromatography-as-the-second-dimension-in-two-dimensional-liquid-chromatography-experiments
#17
Chandan L Barhate, Erik L Regalado, Nathan D Contrella, Joon Lee, Junyong Jo, Alexey A Makarov, Daniel W Armstrong, Christopher J Welch
Chromatographic separation and analysis of complex mixtures of closely related species is one of the most challenging tasks in modern pharmaceutical analysis. In recent years, two-dimensional liquid chromatography (2D-LC) has become a valuable tool for improving peak capacity and selectivity. However, the relatively slow speed of chiral separations has limited the use of chiral stationary phases (CSPs) as the second dimension in 2D-LC, especially in the comprehensive mode. Realizing that the recent revolution in the field of ultrafast enantioselective chromatography could now provide significantly faster separations, we herein report an investigation into the use of ultrafast chiral chromatography as a second dimension for 2D chromatographic separations...
January 23, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28192905/extracting-metallic-nanoparticles-from-soils-for-quantitative-analysis-method-development-using-engineered-silver-nanoparticles-and-sp-icp-ms
#18
D M Schwertfeger, Jessica R Velicogna, Alexander H Jesmer, Selin Saatcioglu, Heather McShane, Richard P Scroggins, Juliska I Princz
The lack of an efficient and standardized method to disperse soil particles and quantitatively subsample the nanoparticulate fraction for characterization analyses is hindering progress in assessing the fate and toxicity of metallic engineered nanomaterials in the soil environment. This study investigates various soil extraction and extract preparation techniques for their ability to remove nanoparticulate Ag from a field soil amended with biosolids contaminated with engineered silver nanoparticles (AgNPs), while presenting a suitable suspension for quantitative single-particle inductively coupled plasma mass spectroscopy (SP-ICP-MS) analysis...
January 30, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28191458/structural-study-of-heterogeneous-biological-samples-by-cryoelectron-microscopy-and-image-processing
#19
REVIEW
H E White, A Ignatiou, D K Clare, E V Orlova
In living organisms, biological macromolecules are intrinsically flexible and naturally exist in multiple conformations. Modern electron microscopy, especially at liquid nitrogen temperatures (cryo-EM), is able to visualise biocomplexes in nearly native conditions and in multiple conformational states. The advances made during the last decade in electronic technology and software development have led to the revelation of structural variations in complexes and also improved the resolution of EM structures. Nowadays, structural studies based on single particle analysis (SPA) suggests several approaches for the separation of different conformational states and therefore disclosure of the mechanisms for functioning of complexes...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28181801/optokinetically-encoded-nanoprobe-based-multiplexing-strategy-for-microrna-profiling
#20
Sungi Kim, Jeong-Eun Park, Woosung Hwang, Jinyoung Seo, Young-Kwang Lee, Jae-Ho Hwang, Jwa-Min Nam
Multiplexed real-time analysis on multiple interacting molecules and particles is needed to obtain information on binding patterns between multiple ligands and receptors, specificity of bond formations and interacting pairs in a complex medi-um, often found in chemical and biological systems, and difference in binding affinity and kinetics for different binding pairs in one solution. In particular, multiplexed profiling of microRNA (miRNA) in a reliable, quantitative manner is of great demand for the use of miRNA in cell biology, biosensing and clinical diagnostic applications, and accurate diagnosis of cancers with miRNA is not possible without detecting multiple miRNA sequences in a highly specific manner...
February 9, 2017: Journal of the American Chemical Society
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