keyword
MENU ▼
Read by QxMD icon Read
search

blood tests

keyword
https://www.readbyqxmd.com/read/29224162/methylation-specific-multiplex-ligation-dependent-probe-amplification-ms-mlpa
#1
Cathy B Moelans, Lilit Atanesyan, Suvi P Savola, Paul J van Diest
This chapter describes a method for the rapid assessment of promoter hypermethylation levels or methylation of imprinted regions in human genomic DNA extracted from various sources using methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA). Multiplex ligation-dependent probe amplification (MLPA) is a powerful and easy-to-perform PCR-based technique that can identify gains, amplifications, losses, deletions, methylation and mutations of up to 55 targets in a single reaction, while requiring only minute quantities of DNA (about 50 ng) extracted from blood, fresh frozen or formalin-fixed paraffin-embedded materials...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29223998/cryptococcal-meningitis-in-apparently-immunocompetent-patients-association-with-idiopathic-cd4-lymphopenia
#2
Samuel Shribman, Alastair Noyce, Sharmilee Gnanapavan, Jonathan Lambourne, Thomas Harrison, Frederick Schon
We present two cases of cryptococcal meningitis in people subsequently diagnosed with idiopathic CD4+ lymphopenia. Both presented with new onset headaches without sinister features and were sent home on multiple occasions from emergency departments. Cryptococcal meningitis in HIV-negative patients poses major diagnostic and management problems; the associated mortality is 9%-27%. We suggest performing blood and cerebrospinal fluid cryptococcal antigen tests in all people with lymphocytic meningitis.
December 9, 2017: Practical Neurology
https://www.readbyqxmd.com/read/29223963/field-evaluation-of-two-point-of-care-tests-for-syphilis-among-men-who-have-sex-with-men-verona-italy
#3
Antonella Zorzi, Maddalena Cordioli, Lorenzo Gios, Paola Del Bravo, Igor Toskin, Rosanna W Peeling, Karel Blondeel, Giuseppe Cornaglia, James Kiarie, Ronald Ballard, Massimo Mirandola
OBJECTIVES: The incidence of HIV and syphilis among men who have sex with men (MSM) in Europe has recently increased. Rapid point-of-care tests (POCTs) for syphilis can improve access to screening. The purpose of this study was to evaluate the performance of two syphilis POCTs compared with laboratory tests among MSM. METHODS: The study was undertaken in Verona, Italy. Asymptomatic MSM, potentially exposed to syphilis, were enrolled prospectively. The POCTs evaluated were SD Bioline Syphilis 3...
December 2017: Sexually Transmitted Infections
https://www.readbyqxmd.com/read/29223938/-prevalence-of-anti-toxoplasma-gondii-antibodies-in-blood-donors-in-buenos-aires-city-from-1967-to-2017
#4
Federico J Kaufer, Liliana A Carral, Matías T Messina, María Vanessa Schneider, Miriam Méndez, Lourdes Herrera, Cristina B Freuler, Ricardo A Durlach
A prevalence study of antibodies anti Toxoplasma gondii in voluntary blood donors who attended the hemotherapy service at the Hospital Alemán during the first four months of the years 1997, 2007 and 2017 was carried out and the results were compared to the study carried out in 1967. The sera where processed with the Sabin Feldman Dye Test. The global average seroprevalence in 1967 was 67.0% (CI95%, 64.4%-69.6%); in 1997, 35.0% (CI95%, 33.3%-38.3%); in 2007, 31.9% (CI95%, 29.6%-34.2%) and in 2017, 21.2% (CI95%, 19...
2017: Medicina
https://www.readbyqxmd.com/read/29223854/baicalein-improves-liver-inflammation-in-diabetic-db-db-mice-by-regulating-hmgb1-tlr4-nf-%C3%AE%C2%BAb-signaling-pathway
#5
Huafeng Yin, Lihao Huang, Ting Ouyang, Lvyi Chen
The current study was designed to investigate the hepatoprotective effects and possible mechanisms of Baicalein (BA) on the diabetic liver injury in vivo and in vitro. The results exhibited that BA significantly restored the blood glucose in oral glucose tolerance test (OGTT) and inhibited the levels of insulin, alanine aminotransferase (ALT), aspartate aminotransaminase (AST), total cholesterol (TC) and triglyceride (TG) in C57BL/KsJ-db/db mice. Moreover, BA strikingly attenuated the extent of steatosis in the liver tissues of diabetic mice...
December 7, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/29223792/prediction-of-persistent-hemodynamic-depression-after-carotid-angioplasty-and-stenting-using-artificial-neural-network-model
#6
Jin Pyeong Jeon, Chulho Kim, Byoung-Doo Oh, Sun Jeong Kim, Yu-Seop Kim
OBJECTIVES: To assess and compare predictive factors for persistent hemodynamic depression (PHD) after carotid artery angioplasty and stenting (CAS) using artificial neural network (ANN) and multiple logistic regression (MLR) or support vector machines (SVM) models. PATIENTS AND METHODS: A retrospective data set of patients (n=76) who underwent CAS from 2007 to 2014 was used as input (training cohort) to a back-propagation ANN using TensorFlow platform. PHD was defined when systolic blood pressure was less than 90mmHg or heart rate was less 50 beats/min that lasted for more than one hour...
December 5, 2017: Clinical Neurology and Neurosurgery
https://www.readbyqxmd.com/read/29223713/diagnostic-values-of-dce-mri-and-dsc-mri-for-differentiation-between-high-grade-and-low-grade-gliomas-a-comprehensive-meta-analysis
#7
Jianye Liang, Dexiang Liu, Peng Gao, Dong Zhang, Hanwei Chen, Changzheng Shi, Liangping Luo
RATIONALE AND OBJECTIVES: This study aimed to collect the studies on the role of dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) and dynamic susceptibility contrast MRI (DSC-MRI) in differentiating the grades of gliomas, and evaluate the diagnostic performances of relevant quantitative parameters in glioma grading. MATERIALS AND METHODS: We systematically searched studies on the diagnosis of gliomas with DCE-MRI or DSC-MRI in Medline, PubMed, China National Knowledge Infrastructure database, Cochrane Library, and Embase published between January 2005 and December 2016...
December 6, 2017: Academic Radiology
https://www.readbyqxmd.com/read/29223710/behavioral-responses-of-cd-1-mice-to-conspecific-and-heterospecific-blood-odors-and-to-a-blood-odor-component
#8
Christian Lahger, Matthias Laska
The odor of blood may have both aversive and attractive properties for mammals, depending on the species of the odor donor and the species perceiving the odor. To better understand the informational content of blood odor for a prey species we assessed behavioral responses of male CD-1 mice (n=60) to the odor of blood of same-sex and opposite-sex conspecifics, of a natural predator of mice (cat), and of a herbivore (horse) and an omnivore (human) non-predator of mice. Further, we assessed their behavior towards the mammalian blood odor component trans-4,5-epoxy-(E)-2-decenal which recent studies have shown to be as attractive to mammalian predators as the odor of real blood...
December 6, 2017: Physiology & Behavior
https://www.readbyqxmd.com/read/29223704/decellularized-heart-ecm-hydrogel-using-supercritical-carbon-dioxide-for-improved-angiogenesis
#9
Yoojin Seo, Youngmee Jung, Soo Hyun Kim
Initial angiogenesis within the first 3 days is critical for healing ischemic diseases such as myocardial infarction. Recently, decellularized extracellular matrix (dECM) has been reported to provide tissue-derived ECM components and can be used as a scaffold for cell delivery for angiogenesis in tissue engineering. Decellularization by various detergents such as sodium dodecyl sulfate (SDS) and triton X-100 can remove the cell nuclei in tissue organs. However, this leads to ECM structure denaturation, decreased presence of various ECM proteins and cytokines, and loss of mechanical properties...
December 6, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29223684/thromboelastometry-as-guidance-for-blood-management-in-patients-undergoing-cardiac-surgery
#10
C Sarrais Polo, A Alonso Morenza, J Rey Picazo, L Álvarez Mercadal, R Beltrao Sial, C Aguilar Lloret
OBJECTIVE: Thromboelastometry is a viscoelastometric method for haemostasis testing in a whole blood sample. The aim of this study was to assess the results of using thromboelastometry as guidance for blood management in cardiac surgery, postoperative adverse events and ICU stay. MATERIAL AND METHOD: Analytical and comparative non-randomised quasi-experimental prospective study with a retrospective control group. The inclusion criteria for the 80 patients were: patients undergoing cardiac surgery who had had prior cardiac surgery, endocarditis surgery or aortic arch surgery...
December 6, 2017: Revista Española de Anestesiología y Reanimación
https://www.readbyqxmd.com/read/29223126/hpev-3-predominated-among-parechovirus-a-positive-infants-during-an-outbreak-in-2013-2014-in-queensland-australia
#11
Donna McNeale, Claire Y T Wang, Katherine E Arden, Ian M Mackay
BACKGROUND: Parechoviruses (HPeV) are endemic seasonal pathogens detected from the respiratory tract, gut, blood and central nervous system (CNS) of children and adults, sometimes in conjunction with a range of acute illnesses. HPeV CNS infection may lead to neurodevelopmental sequelae, especially following infection by HPeV-3, hence screening and genotyping are important to inform epidemiology, aetiology and prognosis. OBJECTIVES: To identify and characterise HPeVs circulating during an outbreak between November 2013 and April 2014 in Queensland, Australia...
December 6, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29223123/differentiation-of-human-blood-from-animal-blood-using-raman-spectroscopy-a-survey-of-forensically-relevant-species
#12
Kyle C Doty, Igor K Lednev
The identification of blood samples is a crucial facet of forensic investigations, particularly for violent crimes. One step in forensic serology (i.e., the analysis of bodily fluids) that is often skipped or overlooked is the determination that a bloodstain is of human or nonhuman origin. Typically, subsequent to identifying a stain as blood using a presumptive blood test, which have the propensity of providing false positive results, the stain is submitted for extraction of a DNA profile to compare with those in a database...
November 27, 2017: Forensic Science International
https://www.readbyqxmd.com/read/29223020/depressive-symptoms-impaired-glucose-metabolism-high-visceral-fat-and-high-systolic-blood-pressure-in-a-subgroup-of-women-with-recent-gestational-diabetes
#13
U Ferrari, F Banning, I Freibothe, K Tröndle, V Sacco, C Wichmann, S Reif, S Moschko, A Potzel, C Gar, N N Sommer, D Popp, J Seissler, A Lechner, H Künzel
Women with gestational diabetes (GDM) are a high risk group for early type 2 diabetes (T2D). Depression is a risk factor for T2D in the general population. We investigated in women after a recent pregnancy with GDM and without a clinical diagnosis of depression, whether mild to moderate depressive symptoms associate with pathologic glucose metabolism. In a cross-sectional analysis, we examined 173 women, 9 ± 3 months after delivery with several psychopathological assessments, 5-point oral glucose tolerance test with insulin, anthropometrics, and laboratory chemistry...
December 5, 2017: Journal of Psychiatric Research
https://www.readbyqxmd.com/read/29222972/factors-associated-with-adverse-pregnancy-outcomes-in-women-with-systematic-lupus-erythematosus
#14
Masashi Deguchi, Yoko Maesawa, Shino Kubota, Mayumi Morizane, Kenji Tanimura, Yasuhiko Ebina, Hideto Yamada
The aim of this prospective study was to determine clinical factors associated with adverse pregnancy outcomes in women with systematic lupus erythematosus (SLE). Fifty-six pregnancies from 46 women with SLE were enrolled. Risk factors for pregnancy loss, premature delivery, hypertensive disorders of pregnancy (HDP), and light-for-date neonate (LFD), were evaluated. Univariate and multivariate logistic regression analyses revealed a history of two or more pregnancy losses before 10 gestational weeks (GW) (OR 11...
November 27, 2017: Journal of Reproductive Immunology
https://www.readbyqxmd.com/read/29222893/postprandial-long-chain-n-3-polyunsaturated-fatty-acid-response-to-krill-oil-and-fish-oil-consumption-in-healthy-women-a-randomised-controlled-single-dose-crossover-study
#15
Hyunsin H Sung, Andrew J Sinclair, Paul A Lewandowski, Xiao Q Su
BACKGROUND AND OBJECTIVES: Krill oil (KO) and fish oil (FO) are good sources of health-benefiting long chain n- 3 polyunsaturated fatty acids (LC n-3 PUFA), EPA and DHA. There are conflicting outcomes on the bioavailability of LC n-3 PUFA from KO compared with FO. This study investigated the postprandial incorporation of LC n- 3 PUFA into plasma lipids following consumption of 5 capsules of KO or FO in comparison with olive oil (OO) control in healthy women. METHODS AND STUDY DESIGN: 10 women (aged 18-45 years) consumed a high-fat (15 g) breakfast, supplemented with 5 g of KO, FO, or OO in a random order with a minimum seven-day washout period between the supplementations...
2018: Asia Pacific Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/29222857/serum-levels-of-the-s100b-protein-and-neuron-specific-enolase-are-associated-with-mortality-in-critically-ill-patients
#16
Izabela Duda, Łukasz Krzych, Halina Jędrzejowska-Szypułka, Joanna Lewin-Kowalik
INTRODUCTION: Evaluation of the prognostic potential of the S100B protein and neuron-specific enolase (NSE) as predictors of mortality in critically ill patients in intensive care units (ICU). MATERIALS AND METHODS: The study was conducted on 62 patients. Basic clinical variables and blood samples for S100B and NSE level testing were obtained during the first four days after admission. Mortality was described as the patient's death during hospitalization in the ICU...
December 10, 2017: Acta Biochimica Polonica
https://www.readbyqxmd.com/read/29222825/outcome-of-sonography-based-minimally-invasive-surgery-for-deep-infiltrating-endometriosis-of-the-ureter-and-urinary-bladder-a-retrospective-cohort-study
#17
Gernot Hudelist, Ayman Tammaa, Kristine Aas-Eng, Lisa Kirchner, Nadja Fritzer, Zoltan Nemeth, Michael Lamche
INTRODUCTION: To evaluate the accuracy of transvaginal sonography for preoperative detection of bladder endometriosis and surgical outcomes regarding fertility and pain symptoms of women with urinary tract endometriosis. MATERIAL AND METHODS: Retrospective cohort study of consecutive patients with urinary tract endometriosis undergoing laparoscopic partial cystectomy and/or ureterolysis/decompression, ureteric resection and end-to-end anastomosis or ureteroneocystostomy for ureteral stenosis and hydronephrosis...
December 8, 2017: Acta Obstetricia et Gynecologica Scandinavica
https://www.readbyqxmd.com/read/29222696/thrombomodulin-favors-leukocyte-microvesicle-fibrinolytic-activity-reduces-netosis-and-prevents-septic-shock-induced-coagulopathy-in-rats
#18
Julie Helms, Raphaël Clere-Jehl, Elsa Bianchini, Pierrick Le Borgne, Mélanie Burban, Fatiha Zobairi, Jean-Luc Diehl, Lelia Grunebaum, Florence Toti, Ferhat Meziani, Delphine Borgel
BACKGROUND: Septic shock-induced disseminated intravascular coagulation is responsible for increased occurrence of multiple organ dysfunction and mortality. Immunothrombosis-induced coagulopathy may contribute to hypercoagulability. We aimed at determining whether recombinant human thrombomodulin (rhTM) could control exaggerated immunothrombosis by studying procoagulant responses, fibrinolysis activity borne by microvesicles (MVs) and NETosis in septic shock. METHODS: In a septic shock model after a cecal ligation and puncture-induced peritonitis (H0), rats were treated with rhTM or a placebo at H18, resuscitated and monitored during 4 h...
December 8, 2017: Annals of Intensive Care
https://www.readbyqxmd.com/read/29222505/a-comparison-of-methods-to-measure-central-and-peripheral-oxytocin-concentrations-in-human-and-non-human-primates
#19
Arthur Lefevre, Raphaëlle Mottolese, Manon Dirheimer, Carmine Mottolese, Jean-René Duhamel, Angela Sirigu
Oxytocin (OT) concentration in the blood is considered to be a marker of its action in the brain. However, two problems have emerged when measuring OT level in the blood. First, it is unclear whether different methods of assessment lead to similar OT values. Second, it is unclear if plasma OT concentrations is informative on what OT does in the brain. To clarify these issues, we collected cerebrospinal fluid (CSF) from the brain ventricle of 25 patients during surgery to compare with plasma OT after simultaneous blood withdrawal...
December 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29222449/detection-of-prions-in-blood-of-cervids-at-the-asymptomatic-stage-of-chronic-wasting-disease
#20
Carlos Kramm, Sandra Pritzkow, Adam Lyon, Tracy Nichols, Rodrigo Morales, Claudio Soto
Chronic wasting disease (CWD) is a rapidly spreading prion disorder affecting captive and free-ranging cervids. The zoonotic potential of CWD is unknown, as well as the mechanism for its highly efficient transmission. A top priority to minimize further spreading of this disease and its potential impact on environmental prion contamination is the development of a non-invasive, sensitive, and specific test for ante-mortem detection of infected animals. Here, we optimized the protein misfolding cyclic amplification (PMCA) assay for highly efficient detection of CWD prions in blood samples...
December 8, 2017: Scientific Reports
keyword
keyword
111786
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"