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https://www.readbyqxmd.com/read/29666886/whole-genome-sequence-and-genome-annotation-of-xanthomonas-citri-pv-mangiferaeindicae-causal-agent-of-bacterial-black-spot-on-mangifera-indica
#1
Feng Liu, Xiong-Chang Ou, Ru-Lin Zhan
A newly isolated strain XC01 was identified as Xanthomonas citri pv. mangiferaeindicae, isolated from an infected mango fruit in Guangxi, China. The complete genome sequence of XC01 was carried out using the PacBio RSII platform. The genome contains a circular chromosome with 3,865,165 bp, 3442 protein-coding genes, 53 tRNAs, and 2 rRNA operons. Phylogenetic analysis revealed that this pathogen is very close to the soybeans bacterial pustule pathogen X. citri pv. glycines CFBP 2526, with a completely different host range...
April 17, 2018: Archives of Microbiology
https://www.readbyqxmd.com/read/29666522/development-of-a-novel-colorimetric-sensor-based-on-alginate-beads-for-monitoring-rainbow-trout-spoilage
#2
Marjan Majdinasab, Seyed Mohammad Hashem Hosseini, Marziyeh Sepidname, Manizheh Negahdarifar, Peiwu Li
Alginate is a non-toxic, renewable, and linear copolymer obtained from the brown algae Laminaria digitata that can be easily shaped into beads. Its good gel forming properties have made it useful for entrapping food and pharmaceutical ingredients. In this study, alginate beads were used in a novel application as a colorimetric sensor in food intelligent packaging. Colorimetric sensor was developed through entrapping red cabbage extract as a pH indicator in alginate beads. The pH indicator beads were used in rainbow trout packaging for monitoring fillets spoilage...
May 2018: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/29665937/-current-understanding-of-myeloproliferative-neoplasm-related-gene-mutations-and-cytokine-review
#3
Zhi-Peng He, Yong Wu
Myeloproliferative neoplasm(MPN) is clonal hematopoietic stem cell disorder characterized by abnormal proliferation and expansion of one or more myeloid lineages. BCR-ABL-negative MPN includes polycythemia vera (PV), essential thrombocythemia (ET) and primary myelofibrosis (PMF). The mutations of JAK2, CALR and MPL genes are involved in the pathogenesis of MPN that provided a more complete molecular diagnostic standard for MPN. More and more new mutated genes related to prognosis of MPN were discovered in the past few years, at same time it was found that cytokines were also involved in the genesis and development of MPN...
April 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/29663178/validation-of-contrast-enhanced-time-resolved-magnetic-resonance-angiography-in-pre-ablation-planning-in-patients-with-atrial-fibrillation-comparison-with-traditional-technique
#4
Tarek Zghaib, Adeel Shahid, Chiara Pozzessere, Kristin K Porter, Linda C Chu, John Eng, Hugh Calkins, Ihab R Kamel, Saman Nazarian, Stefan L Zimmerman
Bolus timing is critical to optimal magnetic resonance angiography (MRA) acquisitions but can be challenging in some patients. Our purpose was to evaluate whether contrast-enhanced time-resolved magnetic resonance angiography (TR-MRA), a dynamic multiphase sequence that does not rely on bolus timing, is a viable alternative method to conventional 3D fast-long angle shot contrast-enhanced magnetic resonance angiography (CE-MRA). Coronal subtracted conventional CE-MRA images in 50 consecutive patients presenting for pre-atrial fibrillation ablation pulmonary venous (PV) mapping were compared with 50 TR-MRA images performed in 50 subsequent patients...
April 16, 2018: International Journal of Cardiovascular Imaging
https://www.readbyqxmd.com/read/29661998/association-and-expression-of-virulence-from-plasmids-of-the-group-b-strain-in-pseudomonas-syringae-pv-eriobotryae
#5
Tran Dang Khanh, Tran Dang Xuan
Pseudomonas syringae pv. eriobotryae causes serious stem canker in loquat ( Eriobotrya japonica ) trees. This study was conducted to determine whether plasmids are involved with its virulence. The strain NAE89, which belonged to the B group, harbored two plasmids at approximately 6.2 and 50 Mdal that caused stem canker and halo leaf spots on loquat plants. Following digestion with Bam HI and ligation into the Bam HI cloning site of the broad range host cosmid pLAFR3, four DNA fragments at 3.8, 6.6, 12.3, and 22...
April 14, 2018: Pathogens
https://www.readbyqxmd.com/read/29661201/feasibility-of-portal-or-superior-mesenteric-vein-resection-and-reconstruction-by-allogeneic-vein-for-pancreatic-head-cancer-a-case-control-study
#6
Xing-Mao Zhang, Jie Zhang, Hua Fan, Qiang He, Ren Lang
BACKGROUND: There are few reports about resection of portal vein (PV)/superior mesenteric vein (SMV) and reconstruction by using allogeneic vein. This case-control study was designed to explore the feasibility and safety of this operation type in patients with T3 stage pancreatic head cancer. METHODS: A total of 42 patients (Group A) underwent PV/SMV resection and reconstruction by using allogeneic vein were 1:1 matched to 42 controls (Group B) with other types of resection and reconstruction...
April 16, 2018: BMC Gastroenterology
https://www.readbyqxmd.com/read/29660912/increased-plasma-viscosity-in-plasma-cell-dyscrasia-and-whole-blood-viscosity-in-polycythemia-vera
#7
Dong Woo Shin, Ja-Yoon Gu, Jun Sik Kim, Jae-Seol Jung, Dong-Yeop Shin, Youngil Koh, Inho Kim, Hyun Kyung Kim
BACKGROUND: Although hyperviscosity syndrome in plasma cell dyscrasia (PCD) and thrombosis in myeloproliferative neoplasm (MPN) are major causes of morbidity and mortality, blood viscosity measurements are often underutilized. OBJECTIVE: This study aimed to characterize whether whole blood viscosity (WBV) or plasma viscosity (PV) could be predictive of hyperviscosity syndrome in PCD and could be elevated in subgroups of MPN. METHODS: A total of 75 patients with hematologic diseases: PCD (n = 26), MPN (n = 25) including polycythemia vera (P...
April 13, 2018: Clinical Hemorheology and Microcirculation
https://www.readbyqxmd.com/read/29660906/viscometer-validation-studies-for-routine-and-experimental-hemorheological-measurements
#8
David Kovacs, Kinga Totsimon, Katalin Biro, Peter Kenyeres, Istvan Juricskay, Gabor Kesmarky, Kalman Toth, Andras Toth
BACKGROUND: Viscosity measurement is challenging due to the internal properties of blood and the artifacts deriving from the various viscometer systems. OBJECTIVE: We aimed to determine the pitfalls of a cone-plate viscometer (Brookfield DV-III Ultra LV) before starting measurements and compare it to our capillary type model (Hemorex Hevimet 40). Effects of sample storage and thermal calibration were assessed as well. METHODS AND RESULTS: Intra-observer variability was studied by 10 replicate measurements of 7 blood samples, mean coefficients of variation were less than 5%...
April 13, 2018: Clinical Hemorheology and Microcirculation
https://www.readbyqxmd.com/read/29660691/proteomic-analysis-of-liquid-biopsy-from-tumor-draining-vein-indicates-that-high-expression-of-exosomal-ecm1-is-associated-with-relapse-in-stage-i-iii-colon-cancer
#9
Sandra Santasusagna, Isabel Moreno, Alfons Navarro, Joan J Castellano, Francisco Martinez, Raquel Hernández, Carmen Muñoz, Mariano Monzo
BACKGROUND: The analysis of exosomes in blood obtained from the tumor-draining mesenteric vein (MV) can identify tumor biomarkers before they reach target organs and form the premetastatic niche where circulating tumor cells can anchor. Our group has recently shown that microRNAs in plasma from the MV-but not the peripheral vein (PV)-have been related to liver metastases in colon cancer (CC) patients. Here we examine the exosomal protein cargo in plasma from the MV and paired PV in 31 CC patients...
April 13, 2018: Translational Oncology
https://www.readbyqxmd.com/read/29660447/the-usefulness-of-ventricular-pacing-during-atrial-fibrillation-ablation-in-a-persistent-left-superior-vena-cava-a-case-report
#10
Sen Matsumoto, Yasuharu Matsunaga-Lee, Nobutaka Masunaga, Yuzuru Takano
A 69-year-old woman with palpitations was referred to our hospital for a second session of atrial fibrillation (AF) catheter ablation. She had a history of AF ablation including pulmonary vein (PV) isolation and persistent left superior vena cava (PLSVC) isolation. Electrophysiologic studies showed the veno-atrial connections that had recovered. After PV isolation was performed, AF was induced by atrial premature contraction (APC) from the PLSVC, and AF storm occurred. During PLSVC isolation, AF was not induced by APC from the PLSVC...
April 13, 2018: Indian Pacing and Electrophysiology Journal
https://www.readbyqxmd.com/read/29660238/heat-shock-transcription-factor-3-regulates-plant-immune-response-through-modulation-of-salicylic-acid-accumulation-and-signaling-in-cassava
#11
Yunxie Wei, Guoyin Liu, Yanli Chang, Chaozu He, Haitao Shi
As the terminal components of signal transduction, heat stress transcription factors (Hsfs) mediate the activation of multiple genes responsive to various stresses. However, the information and functional analysis are very limited in non-model plants, especially in cassava (Manihot esculenta), one of the most important crops in the tropical area. In this study, 32 MeHsfs were identified from cassava genome, the evolutionary tree, gene structures and motifs were also analyzed. Gene expression analysis found that MeHsfs were commonly regulated by Xanthomonas axonopodis pv manihotis (Xam)...
April 16, 2018: Molecular Plant Pathology
https://www.readbyqxmd.com/read/29659148/hydrogen-sulphide-increases-pulmonary-veins-and-atrial-arrhythmogenesis-with-activation-of-protein-kinase-c
#12
Chao-Shun Chan, Yung-Kuo Lin, Yu-Hsun Kao, Yao-Chang Chen, Shih-Ann Chen, Yi-Jen Chen
Hydrogen sulphide (H2 S), one of the most common toxic air pollutants, is an important aetiology of atrial fibrillation (AF). Pulmonary veins (PVs) and left atrium (LA) are the most important AF trigger and substrate. We investigated whether H2 S may modulate the arrhythmogenesis of PVs and atria. Conventional microelectrodes and whole-cell patch clamp were performed in rabbit PV, sinoatrial node (SAN) or atrial cardiomyocytes before and after the perfusion of NaHS with or without chelerythrine (a selective PKC inhibitor), rottlerin (a specific PKC δ inhibitor) or KB-R7943 (a NCX inhibitor)...
April 16, 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29658111/architectonic-characteristics-of-the-visual-thalamus-and-superior-colliculus-in-titi-monkeys
#13
Mary Kl Baldwin, Leah Krubitzer
Titi monkeys are arboreal, diurnal New World monkeys whose ancestors were the first surviving branch of the New World radiation. In the current study, we use cytoarchtectonic and immunohistochemical characteristics to compare subcortical structures associated with visual processing with those of other well-studied primates. Our goal was to appreciate features that are similar across all New World monkeys, and primates in general, versus those features that are unique to titi monkeys and other primate taxa. We examined tissue stained for Nissl substance, cytochrome oxidase (CO), acetylcholinesterase (AChE), calbindin (Cb), parvalbumin (Pv), and vesicular glutamate transporter 2 (VGLUT2) to characterize the superior colliculus, lateral geniculate nucleus, and visual pulvinar...
April 15, 2018: Journal of Comparative Neurology
https://www.readbyqxmd.com/read/29657591/the-lesion-characteristics-assessed-by-lge-mri-after-the-cryoballoon-ablation-and-conventional-radiofrequency-ablation
#14
Jun Kurose, Kunihiko Kiuchi, Koji Fukuzawa, Shumpei Mori, Hirotoshi Ichibori, Hiroki Konishi, Yayoi Taniguchi, Kiyohiro Hyogo, Hiroshi Imada, Hideya Suehiro, Yu-Ichi Nagamatsu, Tomomi Akita, Makoto Takemoto, Ken-Ichi Hirata, Shinsuke Shimoyama, Yoshiaki Watanabe, Tatsuya Nishii, Noriyuki Negi, Katsusuke Kyotani
Background: Rhythm outcomes after the pulmonary vein isolation (PVI) using the cryoballoon (CB) are reported to be excellent. However, the lesions after CB ablation have not been well discussed. We sought to characterize and compare the lesion formation after CB ablation with that after radiofrequency (RF) ablation. Methods: A total of 42 consecutive patients who underwent PVI were enrolled (29 in the CB group and 13 in the RF group). The PVI lesions were assessed by late gadolinium enhancement magnetic resonance imaging 1-3 months after the PVI...
April 2018: Journal of Arrhythmia
https://www.readbyqxmd.com/read/29656958/polymorphisms-in-tlrs-influence-circulating-cytokines-production-in-plasmodium-vivax-malaria-tlr-polymorphisms-influence-cytokine-productions-in-malaria-vivax
#15
Allyson Guimarães Costa, Rajendranath Ramasawmy, Fernando Fonseca Almeida Val, Hiochelson Najibe Santos Ibiapina, Amanda Carvalho Oliveira, Andréa Monteiro Tarragô, Nadja Pinto Garcia, Maria Izabel Ovellar Heckmann, Wuelton Marcelo Monteiro, Adriana Malheiro, Marcus Vinícius Guimarães Lacerda
The efficiency of the immune system has been shaped throughout the evolutionary process allowing adaptations. In a Plasmodium vivax infection, the host attempts to develop an innate immune response to keep in check the parasite that is associated with inflammatory and regulatory processes. Production of pro-inflammatory and regulatory cytokines simultaneously appears to be a balancing mechanism for the host to prevent the onset of severe disease. Changes in the dynamics of circulating cytokines production can influence the pathogenesis, severity of the disease and episodes of recurrent Plasmodium vivax malaria (Pv-malaria)...
April 12, 2018: Cytokine
https://www.readbyqxmd.com/read/29656326/the-impact-of-the-cartosound%C3%A2-image-directly-acquired-from-the-left-atrium-for-integration-in-atrial-fibrillation-ablation
#16
Kenichi Kaseno, Kaori Hisazaki, Kohki Nakamura, Etsuko Ikeda, Kanae Hasegawa, Daisetsu Aoyama, Yuichiro Shiomi, Hiroyuki Ikeda, Tetsuji Morishita, Kentaro Ishida, Naoki Amaya, Hiroyasu Uzui, Hiroshi Tada
PURPOSE: Intracardiac echocardiographic (ICE) imaging might be useful for integrating three-dimensional computed tomographic (CT) images for left atrial (LA) catheter navigation during atrial fibrillation (AF) ablation. However, the optimal CT image integration method using ICE has not been established. METHODS: This study included 52 AF patients who underwent successful circumferential pulmonary vein isolation (CPVI). In all patients, CT image integration was performed after the CPVI with the following two methods: (1) using ICE images of the LA derived from the right atrium and right ventricular outflow tract (RA-merge) and (2) using ICE images of the LA directly derived from the LA added to the image for the RA-merge (LA-merge)...
April 14, 2018: Journal of Interventional Cardiac Electrophysiology: An International Journal of Arrhythmias and Pacing
https://www.readbyqxmd.com/read/29656325/dosing-of-the-second-generation-cryoballoon-using-acute-time-to-pulmonary-vein-isolation-as-an-indicator-of-durable-ablation-in-a-canine-model
#17
Wilber Su, Nicolas Coulombe, Nicole Kirchhof, Erin Grassl, Dan Wittenberger
BACKGROUND: Rigid time-based dosing protocol(s) currently used in the clinic for cryoballoon ablation of atrial fibrillation may be inadequate to guide the circumferential and transmural cryothermal energy transfer across the pulmonary vein (PV) and may result in injury to collateral tissues or electrical gaps between the PV and left atrium (LA). OBJECTIVE: A physiologic endpoint (e.g., acute time-to-PV isolation a.k.a. time-to-effect; TTE) may be effective in the determination of a transmural lesion formation and may allow for individualized ablation dosing across each PV...
April 14, 2018: Journal of Interventional Cardiac Electrophysiology: An International Journal of Arrhythmias and Pacing
https://www.readbyqxmd.com/read/29655755/concentration-of-natural-aroma-compounds-from-fruit-juice-hydrolates-by-pervaporation-in-laboratory-and-semi-technical-scale-part-1-base-study
#18
Anna Dawiec-Liśniewska, Antoni Szumny, Daria Podstawczyk, Anna Witek-Krowiak
The first article of a two-article series presents pervaporation (PV) of volatile aroma compounds from fruit juice hydrolates (plum, apple, blackcurrant and cherry). The purpose of this research was to evaluate the effectiveness of fruit juice hydrolates separation on a laboratory scale (inert gas flow pervaporation system) and semi-technical (vacuum pervaporation system) by means of pervaporation. To create precise matrices for hydrolates before and after pervaporation for each of the separated systems, solid phase microextraction (SPME) technique and the gas chromatography-mass spectrometry (GC-MS) was applied...
August 30, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29653114/adrenal-androgen-measurement-for-assessing-the-selectivity-of-adrenal-venous-sampling-in-primary-aldosteronism
#19
Hongyun Li, Xuebin Zhang, Shanmei Shen, Yan Zhang, Wenjing Zhang, Wenhuan Feng, Ping Li, Dalong Zhu
Adrenal venous sampling (AVS) is the reference standard for primary aldosteronism (PA) subtyping. Cortisol is widely used to assess the success of selective AVS, but it is not always reliable. The aim of the present study was to investigate the usefulness of adrenal androgens, compared to cortisol, in assessing the selectivity of AVS. We consecutively recruited 37 patients with PA undergoing AVS. AVS procedures were performed with the bilateral simultaneous technique without cosyntropin stimulation. We collected two baseline blood samples from 10 patients with a 15-min interval between time -15 (t-15) and time 0 (t0) to measure the variability in the levels of the tested hormones over time...
April 10, 2018: Steroids
https://www.readbyqxmd.com/read/29651577/pathological-margin-clearance-and-survival-after-pancreaticoduodenectomy-in-a-us-and-european-pancreatic-center
#20
Stijn van Roessel, Gyulnara G Kasumova, Omidreza Tabatabaie, Sing Chau Ng, L Bengt van Rijssen, Joanne Verheij, Robert M Najarian, Thomas M van Gulik, Marc G Besselink, Olivier R Busch, Jennifer F Tseng
BACKGROUND: The optimal definition of a margin-negative resection and its exact prognostic significance on survival in resected pancreatic adenocarcinoma remains unknown. This study was designed to assess the relationship between pathological margin clearance, margin type, and survival. METHODS: Patients who underwent pancreaticoduodenectomy with curative intent at two academic institutions, in Amsterdam, the Netherlands, and Boston, Massachusetts, between 2000 and 2014 were retrospectively evaluated...
April 12, 2018: Annals of Surgical Oncology
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