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https://www.readbyqxmd.com/read/29679858/anti-inflammatory-effect-of-apo-9-fucoxanthinone-via-inhibition-of-mapks-and-nf-kb-signaling-pathway-in-lps-stimulated-raw-264-7-macrophages-and-zebrafish-model
#1
Eun-A Kim, Seo-Young Kim, Bo-Ram Ye, Junseong Kim, Seok-Chun Ko, Won Woo Lee, Kil-Nam Kim, Il-Whan Choi, Won-Kyo Jung, Soo-Jin Heo
In this study, we confirmed the anti-inflammatory effect of Apo-9-fucoxanthinone (AF) in in vitro RAW 264.7 cells and in vivo zebrafish model. In lipopolysaccharide (LPS)-stimulated zebrafish, AF significantly decreased the production of reactive oxygen species (ROS), nitric oxide (NO) and cell death. In addition, the mRNA expression of inducible nitric oxide synthase (iNOS), suppressed cyclooxygenase-2 (COX-2) and an inflammatory cytokines; IL-1β, TNF-α were shown reduction. And AF significantly inhibited NO production and expression of iNOS in LPS-stimulated RAW 264...
April 18, 2018: International Immunopharmacology
https://www.readbyqxmd.com/read/29679702/alamandine-attenuates-sepsis-associated-cardiac-dysfunction-via-inhibiting-mapks-signaling-pathways
#2
Peng Li, Xi-Ru Chen, Fei Xu, Chi Liu, Chang Li, Hui Liu, Hui Wang, Wei Sun, Yan-Hui Sheng, Xiang-Qing Kong
Sepsis-induced myocardial dysfunction represents a major cause of death in intensive care units. Alamandine is an important biologically active peptide in the renin-angiotensin system. The present study evaluated whether alamandine improves cardiac dysfunction, inflammation, and apoptosis, and affects the signaling pathways involved in these events. Experiments were carried out in mice treated with lipopolysaccharide (LPS) or alamandine, and in neonatal rat cardiomyocytes. Alamandine increased the ejection fraction and fractional shortening, both of which were decreased upon LPS infusion in mice...
April 18, 2018: Life Sciences
https://www.readbyqxmd.com/read/29676132/-inhibition-of-yiqi-jiedu-formula-on-proliferation-of-nasopharyngeal-carcinoma-cells-by-mapk-erk-signaling-pathway
#3
Jing Hu, Na Dai, Bing-Yan Xu, Fang-Liang Zhou, Ting Lin, Jing-Jing Luo, Jing-Ying Fan, Ying-Chun He
To study the effect of aqueous extracts of Yiqi Jiedu formula (YQ) on the proliferation of CNE2 cells in human nasopharyngeal carcinoma, and investigate its mechanism to provide a new theoretical basis for the clinical application of YQ. CNE2 cells were treated with different concentrations (0.125, 0.25, 0.5, 0.25 g·L⁻¹) of YQ, positive control medicine (cisplatin 4.0 mg·L⁻¹), inhibitor PD98059 (50 μmol·L⁻¹), activator isoproterenol hydrochloride (20 μmol·L⁻¹), activator isoproterenol hydrochloride (ISO)+YQ 0...
March 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29675422/elucidation-on-predominant-pathways-involved-in-the-differentiation-and-mineralization-of-odontoblast-like-cells-by-selective-blockade-of-mitogen-activated-protein-kinases
#4
Jia Tang, Takashi Saito
Aim: To analyze the effect of three mitogen-activated protein kinase (MAPK) inhibitors, namely, SB202190 (p38 inhibitor), SP600125 (JNK inhibitor), and PD98059 (ERK inhibitor) in Dex-stimulated MDPC-23 cell differentiation and mineralization. Methods: Experiment was divided into five groups, control (cells without Dex and inhibitors treatment), Dex (cells with Dex treatment but without inhibitors), Dex + SB202190, Dex + SP600125, and Dex + PD98059. Cell differentiation was assessed by alkaline phosphatase (ALP) activity assay and real time RT-PCR...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29674280/the-chemotherapeutic-effect-of-%C3%AE-2-himachalen-6-ol-in-chemically-induced-skin-tumorigenesis
#5
Hamid E Daaboul, Carole Dagher, Robin I Taleb, Kikki Bodman-Smith, Wassim N Shebaby, Mirvat El-Sibai, Mohamad A Mroueh, Costantine F Daher
β-2-himachalen-6-ol (HC), a novel sesquiterpene derived from Lebanese wild carrot, was shown to possess a remarkable anticancer activity. The present study investigates the in vitro anticancer activity of HC and its effect on papillomas induced using a DMBA/TPA skin carcinogenesis mouse model. HaCaT-ras II-4 epidermal squamous cell viability was assessed using WST-1 kit. Cell cycle was analyzed by flow cytometry, and pro/anti-apoptotic proteins were measured using western blot. Mice papillomas were induced by DMBA and promoted with TPA for 18 weeks...
April 16, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29671254/co-enzyme-q10-upregulates-hsp70-and-protects-chicken-primary-myocardial-cells-under-in-vitro-heat-stress-via-pkc-mapk
#6
Jiao Xu, Shu Tang, Bin Yin, Jiarui Sun, Endong Bao
In this report, we investigate the protective mechanism of co-enzyme Q10 on chicken primary myocardial cells during heat stress. Morphological observations indicate that addition of co-enzyme Q10 protects myocardial cells from heat stress, reduces the damage of mitochondria and nucleus, and decreases the mean number of vacuolated mitochondria. We have previously shown that co-enzyme Q10 can protect myocardial cells by upregulating the expression of Hsp70. Therefore, signaling pathways involved in this process were explored...
April 18, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29669336/clic1-promotes-the-progression-of-gastric-cancer-by-regulating-the-mapk-akt-pathways
#7
Bo-Pei Li, Yuan-Tian Mao, Zhen Wang, Ye-Yang Chen, Ye Wang, Chong-Yu Zhai, Bo Shi, Si-Yu Liu, Jin-Lu Liu, Jun-Qiang Chen
BACKGROUND/AIMS: Chloride intracellular channel 1 (CLIC1), which is a member of the chloride channel protein family, is associated with various human tumors. Recent studies have shown that CLIC1 is involved in the occurrence and development of gastric cancer (GC). However, the exact mechanism remains unclear in GC. METHODS: Effects of CLIC1 on the progression of GC in vivo and in vitro and the potential underlying mechanisms have been investigated by analysing 54 patients with GC, as well as human gastric cell lines SGC-7901 and MGC-803, utilizing proteomics, RT-PCR, Western blotting, flow cytometry, Cell invasion and migration assays and xenograft tumor models...
April 13, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29669300/gzmb-gene-silencing-confers-protection-against-synovial-tissue-hyperplasia-and-articular-cartilage-tissue-injury-in-rheumatoid-arthritis-through-the-mapk-signaling-pathway
#8
Cui-Xia Bao, Hai-Xia Chen, Xue-Jie Mou, Xiang-Kui Zhu, Qi Zhao, Xin-Guang Wang
INTRODUCTION: Rheumatoid arthritis (RA) represents the most commonly occurring inflammatory type of arthritis and is a major cause of disability. Reports have placed emphasis on the potential of, granzyme B (GZMB) as a potentially valuable prognostic marker in early RA, the mechanism of which still remains largely unclear. Thus, the aim of the current study was to investigate the effects GZMB gene silencing influences synovial tissue hyperplasia and articular cartilage tissue injury of RA through the regulation of the MAPK signaling pathway...
April 15, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29666854/casein-glycomacropeptide-hydrolysates-inhibit-pge2-production-and-cox2-expression-in-lps-stimulated-raw-264-7-macrophage-cells-via-akt-mediated-nf-%C3%AE%C2%BAb-and-mapk-pathways
#9
Tiange Li, Dongxiao Gao, Min Du, Xue Cheng, Xueying Mao
A casein glycomacropeptide hydrolysate (GMPH) was found to possess inhibitory activity against lipopolysaccharide (LPS)-induced inflammatory response in our previous study. In the current study, the inhibitory effect and the underlying molecular mechanism of GMPH on inflammatory response in LPS-stimulated RAW264.7 macrophages were further investigated. Results showed that GMPH significantly suppressed LPS-induced intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) production. GMPH reduced the production of prostaglandin E2 (PEG2) and the expression of cyclooxygenase-2 (COX-2) and cytosolic phospholipase A2 (cPLA2) in LPS-stimulated macrophages...
April 18, 2018: Food & Function
https://www.readbyqxmd.com/read/29665555/hesperidin-promotes-programmed-cell-death-by-downregulation-of-nongenomic-estrogen-receptor-signalling-pathway-in-endometrial-cancer-cells
#10
Z B Cincin, B Kiran, Y Baran, B Cakmakoglu
Endometrial carcinoma (EC) is the most common malignant gynecologic tumor in women. EC is thought to be caused by increasing estrogen levels relative to progesterone in the body. Hesperidin (Hsd), a biologically active flavonoid, could be extracted from Citrus species. It has been recently shown that Hsd could exert anticarcinogenic properties in different cancer types. However, the effects of Hsd and its molecular mechanisms on EC remain unclear. In this study, the antiproliferative, apoptotic and genomic effects of Hsd in EC and its underlying mechanisms were identified...
April 14, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29665093/the-mapk-activator-protein-1-signaling-regulates-changes-in-lung-tissue-of-rat-exposed-to-hypobaric-hypoxia
#11
Mrinalini Singh, Seema Yadav, Meetul Kumar, Shweta Saxena, Deepika Saraswat, Anju Bansal, Shashi B Singh
This study reports the role of MAPKs (JNK, ERK, and p38), and activator protein-1 (AP-1) transcription factor in the hypobaric hypoxia induced change in lung tissue. Healthy male Sprague-Dawley rats were exposed to hypobaric hypoxia for 6, 12, 24, 48, 72, and 120 hr. Hypoxia resulted in significant increase in reactive oxygen species (ROS), vascular endothelial growth factor (VEGF) and decreased nitric oxide (NO), these act as signaling molecules for activation of MAPK and also contribute in development of vascular leakage (an indicator of pulmonary edema) as confirmed by histological studies...
April 17, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29662665/molecular-mechanism-of-action-and-potential-biomarkers-of-growth-inhibition-of-synergistic-combination-of-afatinib-and-dasatinib-against-gefitinib-resistant-non-small-cell-lung-cancer-cells
#12
Miao Wang, Alex Yuang-Chi Chang
Epidermal growth factor receptor - tyrosine kinase inhibitor (EGFR-TKI) is the first choice of treatment for advanced non-small cell lung cancer (NSCLC) patients harbouring activating EGFR mutations. However, single agent usually has limited efficacy due to heterogeneous resistant mechanisms of cancer cells. Thus drug combination therapy would offer more benefits by synergistic interactions and avoidance of resistance emergence. In this study, we selected 8 NSCLC cell lines with different genetic characteristics as research models to investigate the efficacy of 4 agents (gefitinib, cetuximab, afatinib and dasatinib) and their combinations...
March 27, 2018: Oncotarget
https://www.readbyqxmd.com/read/29662304/chrysin-suppresses-proliferation-migration-and-invasion-in-glioblastoma-cell-lines-via-mediating-the-erk-nrf2-signaling-pathway
#13
Juan Wang, Handong Wang, Kangjian Sun, Xiaoliang Wang, Hao Pan, Jianhong Zhu, Xiangjun Ji, Xiang Li
Background: Chrysin, an active natural bioflavonoid, has been proven to protect against carcinogenesis. However, the role of chrysin in glioblastoma and the potential molecular mechanisms remain to be elucidated. In our previous study, we found that nuclear factor erythroid 2 (NF-E2)-related factor 2 (Nrf2) is highly expressed in a variety of glioblastoma cell lines associated with the mitogen-activated protein kinase (MAPK) pathway. The aim of this study was to evaluate the antitumor effects of chrysin in glioblastoma cells and how chrysin is related to the MAPK/Nrf2 signaling pathway...
2018: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/29659255/stimulation-of-innate-immune-function-by-panax-ginseng-after-heat-processing
#14
Myoung-Sook Shin, Ji Hoon Song, Pilju Choi, Jong Hun Lee, Song-Yi Kim, Kwang Soon Shin, Jungyeob Ham, Ki Sung Kang
Panax ginseng Meyer has been used for the treatment of immune diseases and for the strengthening the immune function. In this study, we evaluated the innate immune stimulating functions and action mechanisms of white ginseng (WG) and heat-processed ginseng (HPG) in RAW264.7 cells. According to LC-MS analysis results, WG contained typical ginsenosides, such as Rb1, Rc, Rb2, Rd, and Rg1, whereas HPG contained Rg3, Rk1, and Rg5 as well as typical ginsenosides. HPG, not WG enhanced NF-κB/AP-1 activity, cytokine production (IL-6 and TNF-α), and MHC class I and II expression in RAW264...
April 16, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29658909/pretreatment-with-argon-protects-human-cardiac-myocyte-like-progenitor-cells-from-oxygen-glucose-deprivation-induced-cell-death-by-activation-of-akt-and-differential-regulation-of-mapkinases
#15
Hong Qi, Lourdes Soto-Gonzalez, Konstantin A Krychtiuk, Sarah Ruhittel, Christoph Kaun, Walter S Speidl, Attila Kiss, Bruno K Podesser, Shanglong Yao, Klaus Markstaller, Klaus U Klein, Verena Tretter
BACKGROUND: The noble gas argon induces cardioprotection in a rabbit model of myocardial ischemia and reperfusion. However, no studies in human primary cells or subjects have been performed so far. We used human cardiac myocyte-like progenitor cells (HCMs) to investigate the protective effect on the cellular level. METHODS: HCMs were pretreated with 30% or 50% argon before oxygen-glucose deprivation (OGD) and reperfusion. We evaluated apoptotic states by flow cytometry and the activation of mitogen-activated protein kinase (MAPKs) members extracellular signal-regulated kinase (ERK), c-jun N-terminal kinase (JNK), p38 MAPkinase, and protein kinase B (Akt) by Westernblot analysis and by activity assays of downstream transcription factors...
May 2018: Shock
https://www.readbyqxmd.com/read/29658083/antidiabetic-properties-of-low-molecular-weight-bdnf-mimetics-depend-on-the-type-of-activation-of-post-receptor-signaling-pathways
#16
R U Ostrovskaya, S S Yagubova, T A Gudasheva, S B Seredenin
Reduced proliferation and enhanced apoptosis of β cells in diabetes mellitus are associated with a deficiency of brain-derived neurotrophic factor (BDNF). Low-molecular weight compounds similar to different BDNF loops were synthesized at the V. V. Zakusov Research Institute of Pharmacology. They produce a potentiating effect on TrkB phosphorylation, but differently activate post-receptor signaling pathways. We compared their effects on the severity of streptozotocin-induced diabetes mellitus in C57Bl/6 mice...
April 16, 2018: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29657028/monosodium-urate-crystals-induced-icam-1-expression-and-cell-cell-adhesion-in-renal-mesangial-cells-implications-for-the-pathogenesis-of-gouty-nephropathy
#17
Shue-Fen Luo, Chia-Yin Chin, Ling-Jun Ho, Wen-Yi Tseng, Chang-Fu Kuo, Jenn-Haung Lai
BACKGROUND: Renal disease is prevalent in gouty patients and monosodium urate (MSU) crystal deposition in the kidney can be detected in some gouty nephropathy patients. MSU crystals can induce inflammatory events, we investigated the MSU-induced expression of intercellular adhesion molecule (ICAM)-1 on human renal mesangial cells (HRMCs) and the involved signal transduction mechanisms. METHODS: The HRMCs cell line was purchased from ScienCell Research Laboratories...
January 31, 2018: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/29656315/acamprosate-protects-against-adjuvant-induced-arthritis-in-rats-via-blocking-the-erk-mapk-and-nf-%C3%AE%C2%BAb-signaling-pathway
#18
Jun Pan, Rilong Jin, Miaoda Shen, Ronghuan Wu, Sanzhong Xu
Osteoarthritis is a type of joint disease that results from the breakdown of joint cartilage and underlying bone and is believed to be caused by mechanical stress on the joint and low-grade inflammatory processes. Acamprosate significantly ameliorates the pathological features of experimental autoimmune encephalomyelitis due to its anti-inflammatory effect. The aims of the present study were to investigate the anti-arthritis activities of acamprosate and elucidate the underlying mechanisms. Adjuvant-induced arthritis (AIA) was induced by intradermal injection of complete Freund's adjuvant...
April 14, 2018: Inflammation
https://www.readbyqxmd.com/read/29655675/eupatilin-suppresses-the-allergic-inflammatory-response-in-vitro-and-in-vivo
#19
Eun-Hye Song, Kyung-Sook Chung, Yun-Mi Kang, Jong-Hyun Lee, Minho Lee, Hyo-Jin An
INTRODUCTION: Eupatilin, a pharmacologically active ingredient found in Artemisia asiatica, has been reported to have anti-oxidative, anti-inflammatory, and anti-apoptotic activities. However, molecular mechanisms underlying its anti-allergic properties are not yet clear. In this study, we investigated the effects of eupatilin on allergic inflammation in phorbol 12-myristate 13-acetate plus calcium ionophore A23187 (PMACI)-stimulated human mast cells and a compound 48/80-induced anaphylactic shock model...
March 15, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/29653168/purification-partial-characterization-and-inducing-tumor-cell-apoptosis-activity-of-a-polysaccharide-from-ganoderma-applanatum
#20
Dong Zhen, Ling Su, Yue Miao, Fei Zhao, Guihong Ren, Shad Mahfuz, Hui Song
In this study, a homogeneous polysaccharide (designated as GAP-3S) with an average molecular weight of 6.82×105 Da was purified from Ganoderma applanatum. GAP-3S was composed of glucose, galactose, fucose and xylose in the molar ratio of 7.1:2.6:1.1:1. It could significantly inhibit the proliferation of MCF-7 cells in a dose- and time-dependent manner. Flow cytometry analysis indicated that GAP-3S could also induce cell apoptosis. Treatment of MCF-7 cells with GAP-3S lead to the collapse of mitochondrial membrane potential and the accumulation of ROS, up-regulated expression of Bax, cleavage of PARP, p-p38 and p-JNK, and the down-regulated expression of Bcl-2, PARP and p-ERK...
April 10, 2018: International Journal of Biological Macromolecules
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