keyword
MENU ▼
Read by QxMD icon Read
search

cayetanensis

keyword
https://www.readbyqxmd.com/read/29113617/molecular-detection-of-cyclospora-cayetanensis-in-human-stool-specimens-using-unex-based-dna-extraction-and-real-time-pcr
#1
Yvonne Qvarnstrom, Theresa Benedict, Paula L Marcet, Ryan E Wiegand, Barbara L Herwaldt, Alexandre J da Silva
Cyclospora cayetanensis is a coccidian parasite associated with diarrheal illness. In the USA, foodborne outbreaks of cyclosporiasis have been documented almost every year since the mid-1990s. The typical approach used to identify this parasite in human stools is an examination of acid-fast-stained smears under bright-field microscopy. UV fluorescence microscopy of wet mounts is more sensitive and specific than acid-fast staining but requires a fluorescence microscope with a special filter not commonly available in diagnostic laboratories...
November 8, 2017: Parasitology
https://www.readbyqxmd.com/read/28941898/interlaboratory-validation-of-an-improved-method-for-detection-of-cyclospora-cayetanensis-in-produce-using-a-real-time-pcr-assay
#2
Helen R Murphy, Hediye Nese Cinar, Gopal Gopinath, Kathy E Noe, Lacresha D Chatman, Nancy E Miranda, June H Wetherington, Jason Neal-McKinney, Gabrielle S Pires, Elizabeth Sachs, Kristopher J Stanya, Cynthia L Johnson, Fernanda S Nascimento, Monica Santin, Aleksey Molokin, Mansour Samadpour, Harish Janagama, Amy Kahler, Candace Miller, Alexandre J da Silva
A collaborative validation study was performed to evaluate the performance of a new U.S. Food and Drug Administration method developed for detection of the protozoan parasite, Cyclospora cayetanensis, on cilantro and raspberries. The method includes a sample preparation step in which oocysts are recovered from produce using an enhanced produce washing solution containing 0.1% Alconox and a commercially available method to disrupt the C. cayetanensis oocysts and extract DNA. A real-time PCR assay targeting the C...
February 2018: Food Microbiology
https://www.readbyqxmd.com/read/28882168/detection-of-cryptosporidium-parvum-and-cyclospora-cayetanensis-infections-among-people-living-in-a-slum-area-in-kathmandu-valley-nepal
#3
Balkrishna Bhattachan, Jeevan Bahadhur Sherchand, Sarmila Tandukar, Bhim Gopal Dhoubhadel, Leesa Gauchan, Ganesh Rai
OBJECTIVE: The aim of this study is to determine the prevalence of Cyclospora cayetanensis and Cryptosporidium parvum infections among people living a slum in Kathmandu valley, Nepal. RESULTS: Ten different parasites were detected in the stool samples; the prevalence of any parasite was in 27.1% (71/262). The prevalence of C. cayetanensis and C. parvum were 14.1% (10/71) and 5.6% (4/71), respectively. This study showed high prevalence of intestinal parasitic infections along with the coccidian parasites in the slum area of Kathmandu Valley...
September 7, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28697813/multilocus-sequence-typing-and-clonal-population-genetic-structure-of-cyclospora-cayetanensis-in-humans
#4
Junqiang Li, Yankai Chang, K E Shi, Rongjun Wang, Kanda Fu, Shan Li, Jinling Xu, Liting Jia, Zhenxin Guo, Longxian Zhang
To investigate the prevalence of Cyclospora cayetanensis in a longitudinal study and to conduct a population genetic analysis, fecal specimens from 6579 patients were collected during the cyclosporiasis - prevalent seasons in two urban areas of central China in 2011-2015. The overall incidence of C. cayetanensis infection was 1·2% (76/6579): 1·6% (50/3173) in Zhengzhou and 0·8% (26/3406) in Kaifeng (P 0·05). All the isolates clustered in the C. cayetanensis clade based on the small subunit ribosomal RNA gene sequence phylogenetic analysis...
July 12, 2017: Parasitology
https://www.readbyqxmd.com/read/28682770/the-prevalence-of-cyclospora-cayetanensis-and-cryptosporidium-spp-in-turkish-patients-infected-with-hiv-1
#5
Hayriye Kirkoyun Uysal, Gokhan Tolga Adas, Kevser Atalik, Semih Altiparmak, Ozer Akgul, Suat Saribas, Mert Gurcan, Pelin Yuksel, Taner Yildirmak, Bekir Kocazeybek, Tevhide Ziver, Yasar Ali Oner
Opportunistic infections such as cryptosporidiosis and cyclosporiasis are commonly encountered in patients with acquired immunodeficiency syndrome (AIDS). We investigated the existence of opportunistic protozoans that significantly affect the quality of life in HIV-1 infected patients using conventional and molecular methods. The study group comprised 115 HIV-1 positive patients. In the identification of Cyclospora cayetanensis and Cryptosporidium, the formol-ether precipitation method was used and smears were evaluated in optical microscope by staining modified Ziehl-Neelsen (ZN)...
September 26, 2017: Acta Parasitologica
https://www.readbyqxmd.com/read/28648295/detection-and-prevalence-of-protozoan-parasites-in-ready-to-eat-packaged-salads-on-sale-in-italy
#6
Tiziana Caradonna, Marianna Marangi, Federica Del Chierico, Nicola Ferrari, Sofia Reddel, Giorgia Bracaglia, Giovanni Normanno, Lorenza Putignani, Annunziata Giangaspero
To investigate the prevalence of protozoan contamination by Giardia duodenalis, Cryptosporidium spp., Toxoplasma gondii and Cyclospora cayetanensis, in 'ready to eat' (RTE) salads on sale in Italy, 648 packages were purchased from industrial and local brands. Nine individual packages from each brand were collected per month, pooled and subjected to microscopy and molecular analyses. Microscopic examination of 864 slides detected Cryptosporidium spp. but also Blastocystis hominis and Dientamoeba fragilis. Molecular tools identified G...
October 2017: Food Microbiology
https://www.readbyqxmd.com/read/28590822/evaluation-of-an-improved-u-s-food-and-drug-administration-method-for-the-detection-of-cyclospora-cayetanensis-in-produce-using-real-time-pcr
#7
Helen R Murphy, Seulgi Lee, Alexandre J da Silva
Cyclospora cayetanensis is a protozoan parasite that causes human diarrheal disease associated with the consumption of fresh produce or water contaminated with C. cayetanensis oocysts. In the United States, foodborne outbreaks of cyclosporiasis have been linked to various types of imported fresh produce, including cilantro and raspberries. An improved method was developed for identification of C. cayetanensis in produce at the U.S. Food and Drug Administration. The method relies on a 0.1% Alconox produce wash solution for efficient recovery of oocysts, a commercial kit for DNA template preparation, and an optimized TaqMan real-time PCR assay with an internal amplification control for molecular detection of the parasite...
July 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28506035/simultaneous-molecular-detection-of-cryptosporidium-and-cyclospora-from-raw-vegetables-in-korea
#8
Seobo Sim, Jua Won, Jae-Whan Kim, Kyungjin Kim, Woo-Yoon Park, Jae-Ran Yu
Cryptosporidium and Cyclospora are well-known coccidian protozoa that can cause waterborne and foodborne diarrheal illnesses. There have been a few reports regarding contamination in different vegetables with Cryptosporidium, but no data are available regarding the sources of Cyclospora infections in Korea. In the present study, we collected 6 kinds of vegetables (perilla leaves, winter-grown cabbages, chives, sprouts, blueberries, and cherry tomatoes) from July 2014 to June 2015, and investigated contamination by these 2 protozoa using multiplex quantitative real-time PCR...
April 2017: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/28460996/water-borne-protozoa-parasites-the-latin-american-perspective
#9
REVIEW
Félix Manuel Rosado-García, Milena Guerrero-Flórez, Gabriele Karanis, María Del Carmen Hinojosa, Panagiotis Karanis
Health systems, sanitation and water access have certain limitations in nations of Latin America (LA): typical matters of developing countries. Water is often contaminated and therefore unhealthy for the consumers and users. Information on prevalence and detection of waterborne parasitic protozoa are limited or not available in LA. Only few reports have documented in this field during the last forty years and Brazil leads the list, including countries in South America and Mexico within Central America region and Caribbean islands...
July 2017: International Journal of Hygiene and Environmental Health
https://www.readbyqxmd.com/read/28394980/-cyclospora-cayetanensis
#10
Thomas Weitzel, Valeska Vollrath, Lorena Porte
No abstract text is available yet for this article.
February 2017: Revista Chilena de Infectología: órgano Oficial de la Sociedad Chilena de Infectología
https://www.readbyqxmd.com/read/28327845/-intestinal-coccidiosis-in-children-admitted-to-a-hospital-in-peru-and-comparison-of-two-methods-for-detecting-cryptosporidium-spp
#11
Heber Silva-Díaz, Hamer Campos-Flores, Jean Pier Llagas-Linares, Dunalia LLatas-Cancino
A cross-sectional study was done between October 2014 and June 2015 to estimate the frequency of infection due to intestinal coccidiosis in children admitted to a hospital in Peru, and compare the modified acid-fast staining procedure with the enzyme-linked immunoassay (ELISA) method for the detection of Cryptosporidium spp. Coccidia were detected using the modified acid-fast staining procedure and the Cryptosporidium ELISA method (kit r-Biopharm) on seriated stool samples. Out of a total of 325 children, 5...
October 2016: Revista Peruana de Medicina Experimental y Salud Pública
https://www.readbyqxmd.com/read/28168979/first-report-of-tunisian-coastal-water-contamination-by-protozoan-parasites-using-mollusk-bivalves-as-biological-indicators
#12
Khemissa Ghozzi, Marianna Marangi, Roberto Papini, Ibtissem Lahmar, Rafika Challouf, Najoua Houas, Rym Ben Dhiab, Giovanni Normanno, Hamouda Babba, Annunziata Giangaspero
In order to establish seawater contamination by emerging protozoan parasites, we used qPCR to molecularly characterize and evaluate the parasitic burden of Giardia duodenalis, Cryptosporidium spp., Toxoplasma gondii, and Cyclospora cayetanensis in 1255 wild bivalve mollusks collected along the Tunisian coasts. T. gondii, G. duodenalis and C. cayetanensis were detected in 6.9% (99% CI=1.6-12.2%) pools of Ruditapes decussatus. None of the samples were found positive to Cryptosporidium spp.; 6.6% pools of R. decussatus were positive for T...
April 15, 2017: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/27934806/molecular-appraisal-of-intestinal-parasitic-infection-in-transplant-recipients
#13
Pooja Yadav, Shehla Khalil, Bijay Ranjan Mirdha
BACKGROUND & OBJECTIVES: Diarrhoea is the main clinical manifestation caused by intestinal parasitic infections in patients, with special reference to transplant recipients who require careful consideration to reduce morbidity and mortality. Further, molecular characterization of some important parasites is necessary to delineate the different modes of transmission to consider appropriate management strategies. We undertook this study to investigate the intestinal parasitic infections in transplant recipients with or without diarrhoea, and the genotypes of the isolated parasites were also determined...
August 2016: Indian Journal of Medical Research
https://www.readbyqxmd.com/read/27905288/cyclospora-cayetanensis-opportunistic-protozoan-parasite-in-van-province-turkey-a-report-of-seven-cases
#14
Zeynep Taş Cengiz, Yunus Emre Beyhan, Hasan Yılmaz
The aim of this study is to report seven Cyclospora cayetanensis (C.cayetanensis) cases determined in Yuzuncu Yil University Medical Faculty Parasitology Laboratory, Van province, Turkey. In the study native-Lugol, formalin-ethyl acetate and modified acid-fast staining methods were performed to stool samples of the patients sent from outpatient clinics of the hospital. All of the patients infected with C. cayetanensis were older than 15 years. In our cases there were not a history of international travel. Only one of our seven cases was immunosuppressed...
September 2016: Türkiye Parazitolojii Dergisi
https://www.readbyqxmd.com/read/27899155/comparative-sequence-analysis-of-cyclospora-cayetanensis-apicoplast-genomes-originating-from-diverse-geographical-regions
#15
COMPARATIVE STUDY
Hediye Nese Cinar, Yvonne Qvarnstrom, Yuping Wei-Pridgeon, Wen Li, Fernanda S Nascimento, Michael J Arrowood, Helen R Murphy, AhYoung Jang, Eunje Kim, RaeYoung Kim, Alexandre da Silva, Gopal R Gopinath
BACKGROUND: Cyclospora cayetanensis is an emerging coccidian parasite that causes endemic and epidemic diarrheal disease called cyclosporiasis, and this infection is associated with consumption of contaminated produce or water in developed and developing regions. Food-borne outbreaks of cyclosporiasis have occurred almost every year in the USA since the 1990s. Investigations of these outbreaks are currently hampered due to lack of molecular epidemiological tools for trace back analysis...
November 29, 2016: Parasites & Vectors
https://www.readbyqxmd.com/read/27853120/multiplex-touchdown-pcr-to-simultaneously-detect-cryptosporidium-parvum-giardia-lamblia-and-cyclospora-cayetanensis-the-major-causes-of-traveler-s-diarrhea
#16
Ji-Hun Shin, Sang-Eun Lee, Tong Soo Kim, Da-Won Ma, Jong-Yil Chai, Eun-Hee Shin
This study aimed to develop a multiplex-touchdown PCR method to simultaneously detect 3 species of protozoan parasites, i.e., Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis, the major causes of traveler's diarrhea and are resistant to standard antimicrobial treatments. The target genes included the Cryptosporidium oocyst wall protein for C. parvum, Glutamate dehydrogenase for G. lamblia, and 18S ribosomal RNA (18S rRNA) for C. cayetanensis. The sizes of the amplified fragments were 555, 188, and 400 bps, respectively...
October 2016: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/27553132/evaluation-of-library-preparation-methods-for-illumina-next-generation-sequencing-of-small-amounts-of-dna-from-foodborne-parasites
#17
Fernanda S Nascimento, Yuping Wei-Pridgeon, Michael J Arrowood, Delynn Moss, Alexandre J da Silva, Eldin Talundzic, Yvonne Qvarnstrom
Illumina library preparation methods for ultra-low input amounts were compared using genomic DNA from two foodborne parasites (Angiostrongylus cantonensis and Cyclospora cayetanensis) as examples. The Ovation Ultralow method resulted in libraries with the highest concentration and produced quality sequencing data, even when the input DNA was in the picogram range.
November 2016: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/27433881/multilocus-sequence-typing-tool-for-cyclospora-cayetanensis
#18
Yaqiong Guo, Dawn M Roellig, Na Li, Kevin Tang, Michael Frace, Ynes Ortega, Michael J Arrowood, Yaoyu Feng, Yvonne Qvarnstrom, Lin Wang, Delynn M Moss, Longxian Zhang, Lihua Xiao
Because the lack of typing tools for Cyclospora cayetanensis has hampered outbreak investigations, we sequenced its genome and developed a genotyping tool. We observed 2 to 10 geographically segregated sequence types at each of 5 selected loci. This new tool could be useful for case linkage and infection/contamination source tracking.
August 2016: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/27417091/prevalence-of-enteric-protozoan-oocysts-with-special-reference-to-sarcocystis-cruzi-among-fecal-samples-of-diarrheic-immunodeficient-patients-in-iran
#19
Mahmoud Agholi, Shahrbanou Naderi Shahabadi, Mohammad Hossein Motazedian, Gholam Reza Hatam
The genus Sarcocystis is not usually considered as an important enteric pathogen in immune compromised patients. It might be expected that species for which humans are the final host (Sarcocystis hominis and Sarcocystis suihominis as well as possibly others) would be encountered increasingly often in immunodeficient persons. This study aimed to address how to detect and differentiate Sarcocystis oocysts and/or sporocysts from enteric protozoans in the diarrheal samples of immunodeficient patients in Shiraz, Iran...
June 2016: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/27338018/replication-and-maintenance-of-the-plasmodium-falciparum-apicoplast-genome
#20
REVIEW
Morgan E Milton, Scott W Nelson
Members of the phylum Apicomplexa are responsible for many devastating diseases including malaria (Plasmodium spp.), toxoplasmosis (Toxoplasma gondii), babesiosis (Babesia bovis), and cyclosporiasis (Cyclospora cayetanensis). Most Apicomplexans contain a unique and essential organelle called the apicoplast. Derived from an ancient chloroplast, the apicoplast replicates and maintains a 35 kilobase (kb) circular genome. Due to its essential nature within the parasite, drugs targeted to proteins involved in DNA replication and repair of the apicoplast should be potent and specific...
August 2016: Molecular and Biochemical Parasitology
keyword
keyword
111731
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"