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GPR 30

Vincent Portegijs, Lars-Eric Fielmich, Matilde Galli, Ruben Schmidt, Javier Muñoz, Tim van Mourik, Anna Akhmanova, Albert J R Heck, Mike Boxem, Sander van den Heuvel
During cell division, the mitotic spindle segregates replicated chromosomes to opposite poles of the cell, while the position of the spindle determines the plane of cleavage. Spindle positioning and chromosome segregation depend on pulling forces on microtubules extending from the centrosomes to the cell cortex. Critical in pulling force generation is the cortical anchoring of cytoplasmic dynein by a conserved ternary complex of Gα, GPR-1/2, and LIN-5 proteins in C. elegans (Gα-LGN-NuMA in mammals). Previously, we showed that the polarity kinase PKC-3 phosphorylates LIN-5 to control spindle positioning in early C...
October 2016: PLoS Genetics
Alejandro Orlowski, Verónica C De Giusti, María C Ciancio, María S Espejo, Ernesto A Aiello
The sodium/bicarbonate cotransporter (NBC) transports extracellular Na(+) and HCO3(-) into the cytoplasm upon intracellular acidosis, restoring the acidic pHi to near neutral values. Two different NBC isoforms have been described in the heart, the electroneutral NBCn1 (1Na(+):1HCO3(-)) and the electrogenic NBCe1 (1Na(+):2HCO3(-)). Certain non-genomic effects of aldosterone (Ald) were due to an orphan G protein-couple receptor 30 (GPR30). We have recently demonstrated that Ald activates GPR30 in adult rat ventricular myocytes, which transactivates the epidermal growth factor receptor (EGFR) and in turn triggers a reactive oxygen species (ROS)- and PI3K/AKT-dependent pathway, leading to the stimulation of NBC...
June 1, 2016: Channels
Karsen Seto, Minh Hoang, Thaddeus Santos, Mausumi Bandyopadhyay, Mark S Kindy, Subhajit Dasgupta
The non-genomic membrane bound oestrogen receptor (mER) regulates intracellular signals through receptor-ligand interactions. The mER, along with G-protein coupled oestrogen receptor GPR 30 (GPER), induces diverse cell signalling pathways in murine lymphocytes. The mER isoform ER-alpha46 has recently been demonstrated in human B and T lymphocytes as an analogue receptor for chemokine CCL18, the signalling events of which are not clearly understood. Ligand-induced mER and GPER signalling events are shared with BCR, CD19 mediated intracellular signalling through phospholipase C, PIP2/IP3/PI3 mediated activation of Akt, MAP kinase, and mTOR...
July 2016: International Journal of Biochemistry & Cell Biology
Miltiadis Alamaniotis, Dimitrios Bargiotas, Lefteri H Tsoukalas
Integration of energy systems with information technologies has facilitated the realization of smart energy systems that utilize information to optimize system operation. To that end, crucial in optimizing energy system operation is the accurate, ahead-of-time forecasting of load demand. In particular, load forecasting allows planning of system expansion, and decision making for enhancing system safety and reliability. In this paper, the application of two types of kernel machines for medium term load forecasting (MTLF) is presented and their performance is recorded based on a set of historical electricity load demand data...
2016: SpringerPlus
Hongling Dong, Chaoyang Zhu, Jingyi Chen, Xing Ye, Yu-Ping Huang
Maltocin P28 is a phage-tail like bacteriocin produced by Stenotrophomonas maltophilia P28. The ORF8 of maltocin P28 gene cluster is predicted to encode an endolysin and we name it endolysin P28. Sequence analysis revealed that it contains the lysozyme_like superfamily conserved domain. Endolysin P28 has the four consensus motifs as that of Escherichia coli phage lambda gpR. In this study, endolysin P28 was expressed in E. coli BL21 (DE3) and purified with a C-terminal oligo-histidine tag. The antibacterial activity of endolysin P28 increased as the temperature rose from 25 to 45°C...
2015: Frontiers in Microbiology
Fei Wang, Qing Yang, Sarah Huesman, Min Xu, Xiaoming Li, Danwen Lou, Stephen C Woods, Corina Marziano, Patrick Tso
Both glucagon-like peptide-1 (GLP-1) and apolipoprotein A-IV (apoA-IV) are produced from the gut and enhance postprandial insulin secretion. This study investigated whether apoA-IV regulates nutrient-induced GLP-1 secretion and whether apoA-IV knockout causes compensatory GLP-1 release. Using lymph-fistula-mice, we first determined lymphatic GLP-1 secretion by administering apoA-IV before an intraduodenal Ensure infusion. apoA-IV changed neither basal nor Ensure-induced GLP-1 secretion relative to saline administration...
October 15, 2015: American Journal of Physiology. Gastrointestinal and Liver Physiology
Dandan Shang, Zheng Li, Zhuxia Zhu, Huamei Chen, Lujun Zhao, Xudong Wang, Yan Chen
Flavonoids are structurally similar to steroid hormones, particularly estrogens, and therefore have been studied for their potential effects on hormone-dependent cancers. Baicalein is the primary flavonoid derived from the root of Scutellaria baicalensis Georgi. In the present study, we investigated the effects of baicalein on 17β-estradiol (E2)-induced migration, adhesion and invasion of MCF-7 and SK-BR-3 breast cancer cells. The results demonstrated that baicalein suppressed E2-stimulated wound-healing migration and cell‑Matrigel adhesion, and ameliorated E2-promoted invasion across a Matrigel-coated Transwell membrane...
April 2015: Oncology Reports
Norbert Nass, Thomas Kalinski
Tamoxifen is still the most frequently used antiestrogen for the treatment of patients with premenopausal, estrogen receptor positive breast cancer. However, in 20-30% of these cases, tamoxifen therapy fails due to an existing or developing resistance. The prediction of tamoxifen resistance by appropriate biomarker analysis and the development of novel therapies for tamoxifen resistance in premenopausal breast cancer is, therefore, an important goal of ongoing research. Tamoxifen resistance is associated with altered estrogen receptor expression especially on the plasma membrane, including the alternative G-protein coupled receptor GPR-30 (GPER) and estrogen receptor splice products, such as ERα36...
March 2015: Pathology, Research and Practice
Panos Panagos, Cristiano Ballabio, Pasquale Borrelli, Katrin Meusburger, Andreas Klik, Svetla Rousseva, Melita Perčec Tadić, Silas Michaelides, Michaela Hrabalíková, Preben Olsen, Juha Aalto, Mónika Lakatos, Anna Rymszewicz, Alexandru Dumitrescu, Santiago Beguería, Christine Alewell
Rainfall is one the main drivers of soil erosion. The erosive force of rainfall is expressed as rainfall erosivity. Rainfall erosivity considers the rainfall amount and intensity, and is most commonly expressed as the R-factor in the USLE model and its revised version, RUSLE. At national and continental levels, the scarce availability of data obliges soil erosion modellers to estimate this factor based on rainfall data with only low temporal resolution (daily, monthly, annual averages). The purpose of this study is to assess rainfall erosivity in Europe in the form of the RUSLE R-factor, based on the best available datasets...
April 1, 2015: Science of the Total Environment
Suravi Majumder, Sumana Das, Sujata Roy Moulik, Buddhadev Mallick, Puja Pal, Dilip Mukherjee
GPR-30, now named as GPER (G protein-coupled estrogen receptor) was first identified as an orphan receptor and subsequently shown to be required for estrogen-mediated signaling in certain cancer cells. Later studies demonstrated that GPER has the characteristics of a high affinity estrogen membrane receptor on Atlantic croaker and zebra fish oocytes and mediates estrogen inhibition of oocyte maturation in these two distantly related teleost. To determine the broad application of these findings to other teleost, expression of GPER mRNA and its involvement in 17β-estradiol mediated inhibition of oocyte maturation in other cyprinid, Cyprinus carpio was investigated...
January 15, 2015: General and Comparative Endocrinology
Cinthia Santos Miotto de Amorim, Mauro Emilio Conforto Gracitelli, Amélia Pasqual Marques, Vera Lúcia Dos Santos Alves
OBJECTIVE: The purpose of this study was to assess the effectiveness of global postural reeducation (GPR) relative to segmental exercises (SE) in the treatment of scapular dyskinesis (SD) associated with neck pain. METHODS: Participants with SD and neck pain (n = 30) aged 18 to 65 years were randomly assigned to one of two groups: GPR and SE (stretching exercises). The upper extremity was assessed using the Disabilities of the Arm, Shoulder, and Hand questionnaire; function of the neck was estimated using the Neck Disability Index; pain severity was measured using a visual analogical scale; and health-related quality of life was assessed using the Short Form-12...
July 2014: Journal of Manipulative and Physiological Therapeutics
Camila Moraes, Hércules J Rebelato, Maria Esmeria C Amaral, Thais Marangoni Resende, Eduarda V C Silva, Marcelo A M Esquisatto, Rosana Catisti
Consequences of gestational protein restriction (GPR) on liver metabolism in rat offspring were investigated. Pregnant dams were divided into groups: normal (NP, 17% casein) or low-protein diet (LP, 6% casein). Livers were collected from 30-day-old offspring (d30) for analysis or isolation of mitochondria. At d30, hepatic and muscle glycogen was increased in LP group. Mitochondrial swelling and oxygen uptake (recorded with a Clark-type electrode) were significantly reduced in NP female and LP pups. Thiobarbituric acid reactive substances production was lower in females (NP or LP), suggesting significant inhibition of lipid peroxidation...
September 2014: Journal of Physiological Sciences: JPS
Tao Li, Xudong Xiao, Jie Zhang, Yu Zhu, Yi Hu, Jiatao Zang, Kaizhi Lu, Tiande Yang, Hengjiang Ge, Xiaoyong Peng, Dan Lan, Liangming Liu
Several medical conditions exhibit age- and sex-based differences. Whether or not traumatic shock exhibits such differences with regard to vascular responsiveness is not clear. In a cohort of 177 healthy subjects and 842 trauma patients (21-82 years) as well as different ages (4, 8, 10, 14, 18, and 24 wk; 1 and 1.5 years) and sexes of Sprague-Dawley normal and traumatic shock rats, the age- and sex-based differences of vascular responsiveness and the underlying mechanisms were investigated. Middle-aged and young women as well as female rats of reproductive age had higher vascular responsiveness in the normal condition and a lower decrease in vascular responsiveness after traumatic shock than older men and male rats of identical age...
April 15, 2014: American Journal of Physiology. Heart and Circulatory Physiology
Chengliu Gou, Zhaohui Yang, Jing Huang, Huiling Wang, Haiyin Xu, Like Wang
Anaerobic co-digestion of waste activated sludge and food waste was investigated semi-continuously using continuously stirred tank reactors. Results showed that the performance of co-digestion system was distinctly influenced by temperature and organic loading rate (OLR) in terms of gas production rate (GPR), methane yield, volatile solids (VS) removal efficiency and the system stability. The highest GPR at 55 °C was 1.6 and 1.3 times higher than that at 35 and 45 °C with the OLR of 1 g VSL(-1)d(-1), and the corresponding average CH₄ yields were 0...
June 2014: Chemosphere
Yang Li, Yan Chen, Zhu-Xia Zhu, Xiao-Hong Liu, Li Yang, Lei Wan, Ting-Wen Lei, Xu-Dong Wang
Over-expression of cleaved cyclin E in breast tumors is closely associated with tumor progression and resistance to antiestrogens. 17β-Estradiol (E2) has been recently shown to induce cyclin E processing in breast cancer cells. Tamoxifen has been used in patients with estrogen-sensitive breast cancer, yet resistance to antiestrogens and recurrence will appear in some of the patients after its continued use. We therefore addressed possible effects of tamoxifen on the generation of cleaved cyclin E and its signal mechanism(s) in estrogen-responsive MCF-7 breast cancer cells that express both G protein-coupled protein (GPR) 30 and estrogen receptor α (ERα)...
July 5, 2013: Toxicology
Shoulei Kang, Ying Liu, Di Sun, Chunle Zhou, Aiying Liu, Chuanying Xu, Yanling Hao, Dongye Li, Changdong Yan, Hong Sun
G protein-coupled receptor (GPR) 30 is a novel estrogen receptor. Recent studies suggest that activation of the GPR30 confers rapid cardioprotection in isolated rat heart. It is unknown whether chronic activation of GPR30 is beneficial or not for heart failure. In this study we investigated the cardiac effect of sustained activation or inhibition of GPR30. Female Sprague-Dawley rats were divided into 7 groups #2Q1: sham surgery (Sham), bilateral ovariectomy (OVX), OVX+estrogen (E(2)), OVX+isoproterenol (ISO), OVX+ISO+G-1, OVX+ISO+E(2)+G15, OVX+ISO+E(2)...
2012: PloS One
E Farfour, J Leto, M Barritault, C Barberis, J Meyer, B Dauphin, A-S Le Guern, A Leflèche, E Badell, N Guiso, A Leclercq, A Le Monnier, M Lecuit, V Rodriguez-Nava, E Bergeron, J Raymond, S Vimont, E Bille, E Carbonnelle, H Guet-Revillet, H Lécuyer, J-L Beretti, C Vay, P Berche, A Ferroni, X Nassif, O Join-Lambert
Matrix-associated laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is a rapid and simple microbial identification method. Previous reports using the Biotyper system suggested that this technique requires a preliminary extraction step to identify Gram-positive rods (GPRs), a technical issue that may limit the routine use of this technique to identify pathogenic GPRs in the clinical setting. We tested the accuracy of the MALDI-TOF MS Andromas strategy to identify a set of 659 GPR isolates representing 16 bacterial genera and 72 species by the direct colony method...
August 2012: Journal of Clinical Microbiology
T Soga, S L Dalpatadu, D W Wong, I S Parhar
Synthetic glucocorticoid (dexamethasone; DEX) treatment during the neonatal stage is known to affect reproductive activity. However, it is still unknown whether neonatal stress activates gonadotropin-inhibitory hormone (GnIH) synthesizing cells in the dorsomedial hypothalamus (DMH), which could have pronounced suppressive action on gonadotropin-releasing hormone (GnRH) neurons, leading to delayed pubertal onset. This study was designed to determine the effect of neonatal DEX (1.0mg/kg) exposure on reproductive maturation...
August 30, 2012: Neuroscience
James W Simpkins, Meharvan Singh, Courtney Brock, Anne M Etgen
This review is intended to assess the state of current knowledge on the role of estrogen receptors (ERs) in the neuroprotective effects of estrogens in models for acute neuronal injury and death. We evaluate the overall evidence that estrogens are neuroprotective in acute injury and critically assess the role of ERα, ERβ, GPR 30, and nonreceptor-mediated mechanisms in these robust neuroprotective effects of this ovarian steroid hormone. We conclude that all three receptors, as well as nonreceptor-mediated mechanisms can be involved in neuroprotection, depending on the model used, the level of estrogen administrated, and the mode of administration of the steroid...
2012: Neuroendocrinology
Serge Carreau, Helene Bouraima-Lelong, Christelle Delalande
The biosynthesis of steroids and the production of spermatozoa are two major functions of the mammalian testis which are tightly controlled by gonadotropins and numerous locally produced factors. Among these are the estrogens that are produced within the seminiferous epithelium via the irreversible transformation of androgens (C19) into estrogens (C18) by aromatase. We have recently reported that male germ cells are the new source of estrogens in the testis. For instance, estrogen receptors (ER) are found mainly in spermatids that give rise to spermatozoa...
April 2011: Spermatogenesis
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