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epithelial thickness mapping

Menglin Wu, Wen Fan, Qiang Chen, Zhenlong Du, Xiaoli Li, Songtao Yuan, Hyunjin Park
Assessment of serous retinal detachment plays an important role in the diagnosis of central serous chorioretinopathy (CSC). In this paper, we propose an automatic, three-dimensional segmentation method to detect both neurosensory retinal detachment (NRD) and pigment epithelial detachment (PED) in spectral domain optical coherence tomography (SD-OCT) images. The proposed method involves constructing a probability map from training samples using random forest classification. The probability map is constructed from a linear combination of structural texture, intensity, and layer thickness information...
September 1, 2017: Biomedical Optics Express
Smail Hadj-Rabia, Gaelle Brideau, Yasser Al-Sarraj, Rachid C Maroun, Marie-Lucile Figueres, Stéphanie Leclerc-Mercier, Eric Olinger, Stéphanie Baron, Catherine Chaussain, Dominique Nochy, Rowaida Z Taha, Bertrand Knebelmann, Vandana Joshi, Patrick A Curmi, Marios Kambouris, Rosa Vargas-Poussou, Christine Bodemer, Olivier Devuyst, Pascal Houillier, Hatem El-Shanti
PurposeWe aimed to identify the genetic cause to a clinical syndrome encompassing hypohidrosis, electrolyte imbalance, lacrimal gland dysfunction, ichthyosis, and xerostomia (HELIX syndrome), and to comprehensively delineate the phenotype.MethodsWe performed homozygosity mapping, whole-genome sequencing, gene sequencing, expression studies, functional tests, protein bioinformatics, and histological characterization in two unrelated families with HELIX syndrome.ResultsWe identified biallelic missense mutations (c...
August 3, 2017: Genetics in Medicine: Official Journal of the American College of Medical Genetics
Qiao Lu, Yongjun Hu, Jiaxin Chen, Shan Jin
Mass spectrometry imaging (MSI) is an innovative and powerful tool in biomedical research. It is well-known that folic acid (FA) has a high affinity for folic acid receptor (FR), which is overexpressing in epithelial cancer. Herein, we propose a novel method to diagnose cancer through direct mapping of the label-free FA spatial distribution in tissue sections by state-of-the-art laser desorption postionization-mass spectrometry imaging (LDPI-MSI). Compared with other tumor imaging methods, such as fluorescence imaging, photoacoustic imaging (PAI), magnetic resonance imaging (MRI), and micro-SPECT/CT, complicated synthesis and labeling processes are not required...
August 2, 2017: Analytical Chemistry
Menglin Wu, Qiang Chen, Xiaojun He, Ping Li, Wen Fan, Songtao Yuan, Hyunjin Park
OBJECTIVE: Accurate segmentation of neurosensory retinal detachment (NRD)-associated subretinal fluid in spectral domain optical coherence tomography (SD-OCT) is vital for the assessment of central serous chorioretinopathy (CSC). A novel two-stage segmentation algorithm was proposed, guided by Enface fundus imaging. METHODS: In the first stage, Enface fundus image was segmented using thickness map prior to detecting the fluid-associated abnormalities with diffuse boundaries...
April 18, 2017: IEEE Transactions on Bio-medical Engineering
Marcella Q Salomão, Ana Luisa Hofling-Lima, Bernardo T Lopes, Ana Laura C Canedo, Daniel G Dawson, Rui Carneiro-Freitas, Renato Ambrósio
PURPOSE OF REVIEW: Refractive surgery has stimulated considerable progress in corneal and anterior segment imaging, and optical characterization of the eye. From front surface corneal topography, we evolved to three-dimensional corneal tomography with limbus to limbus characterization of the front and back corneal surfaces and pachymetric mapping. Corneal anatomical evaluation has further evolved to layered or segmental tomography with the ability to characterize corneal epithelial thickness profile and the elevation of stromal front surface...
July 2017: Current Opinion in Ophthalmology
Nicolas Schierbaum, Johannes Rheinlaender, Tilman E Schäffer
Malignant transformation drastically alters the mechanical properties of the cell and its response to the surrounding cellular environment. We studied the influence of the physical contact between adjacent cells in an epithelial monolayer on the viscoelastic behavior of normal MCF10A, non-invasive cancerous MCF7, and invasive cancerous MDA-MB-231 human breast cells. Using an atomic force microscopy (AFM) imaging technique termed force clamp force mapping (FCFM) to record images of the viscoelastic material properties, we found that normal MCF10A cells are stiffer and have a lower fluidity at confluent than at sparse density...
April 7, 2017: Acta Biomaterialia
Julie M Schallhorn, Maolong Tang, Yan Li, Derek J Louie, Winston Chamberlain, David Huang
PURPOSE: To distinguish between corneal ectasia and contact lens-related warpage by characteristic patterns on corneal topography and optical coherence tomography (OCT) epithelial thickness maps. SETTING: Casey Eye Institute, Portland, Oregon, USA. DESIGN: Prospective and retrospective case series. METHODS: Axial and mean power maps were obtained on corneal topography systems. Epithelial thickness maps were generated using RTVue OCT...
January 2017: Journal of Cataract and Refractive Surgery
Aysun Sanal Dogan, Mutlu Acar, Mustafa Kosker, Nese Arslan, Canan Gurdal
PURPOSE: To evaluate whether the corneal epithelial thickness (CET) maps obtained by optical coherence tomography (OCT) of the congenital myogenic eyelid ptosis differ from controls. METHODS: CET maps of 13 patients with congenital myogenic eyelid ptosis (m/f = 9/4; mean age 20.5 ± 7.5) and randomly selected one eye of 13 controls (m/f = 7/6; mean age 21.5 ± 6.5) were investigated. Three pachymetry scans of each eye were performed by OCT (RTVue-XR, Optovue Inc...
December 26, 2016: International Ophthalmology
Thibaut Van Acker, Stijn J M Van Malderen, Marjolein Van Heerden, James Eric McDuffie, Filip Cuyckens, Frank Vanhaecke
Two-dimensional elemental mapping (bioimaging) via laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) was performed on 5 μm thick formalin-fixed, paraffin-embedded kidney tissue sections from Cynomolgus monkeys administered with increasing pharmacological doses of cisplatin. Laterally resolved pixels of 1 μm were achieved, enabling elemental analysis on a (sub-)cellular level. Zones of high Pt response were observed in the renal cortex, where proximal tubules are present, the epithelium of which is responsible for partial reabsorption of cisplatin...
November 16, 2016: Analytica Chimica Acta
Shiro Jimi, Francesco De Francesco, Giuseppe A Ferraro, Michele Riccio, Shuuji Hara
The mouse excisional dorsal full-thickness wound model with a silicon splint fixed on the skin has been widely used to mimic human wound healing. However, the method cannot accurately quantify dermal remodeling, since the initial point of epithelialization on the wound surface is unclear. To overcome this limitation, we have developed a novel mouse excisional wound model to assess the degree of epithelial extension and regeneration, using a plastic ring-shaped splint fixed beneath the surrounding epidermal tissue...
June 2017: Journal of Cellular Physiology
Ronald H Silverman, Raksha Urs, Arindam RoyChoudhury, Timothy J Archer, Marine Gobbe, Dan Z Reinstein
PURPOSE: Scanning Scheimpflug provides information regarding corneal thickness and 2-surface topography while arc-scanned high-frequency ultrasound allows depiction of the epithelial and stromal thickness distributions. Both techniques are useful in detection of keratoconus. Our aim was to develop and test a keratoconus classifier combining information from both methods. METHODS: We scanned 111 normal and 30 clinical keratoconus subjects with Artemis-1 and Pentacam data...
March 10, 2017: European Journal of Ophthalmology
S Catalan, L Cadarso, F Esteves, J Salgado-Borges, M Lopez, C Cadarso
Purpose. To compare the characteristics of asymmetric keratoconic eyes and normal eyes by Fourier domain optical coherence tomography (OCT) corneal mapping. Methods. Retrospective corneal and epithelial thickness OCT data for 74 patients were compared in three groups of eyes: keratoconic (n = 22) and normal fellow eyes (n = 22) in patients with asymmetric keratoconus and normal eyes (n = 104) in healthy subjects. Areas under the curve (AUC) of receiver operator characteristic (ROC) curves for each variable were compared across groups to indicate their discrimination capacity...
2016: Journal of Ophthalmology
Dan Z Reinstein, Marine Gobbe, Timothy J Archer, Glenn I Carp
PURPOSE: Case series report of normal-appearing eyes after hyperopic LASIK exhibiting diurnal shift in refractive error found to correlate with diurnal shifts in epithelial thickness profile. METHODS: Patients complaining of diurnal shift in vision during the course of the day with more hyperopia on waking and decreasing hyperopia in the evening in the absence of abnormal epithelial or slit-lamp findings were examined. Diurnal concomitant measurement of refraction, topography, and Artemis very high-frequency digital ultrasound scanning (VHFDU) (ArcScan Inc...
June 1, 2016: Journal of Refractive Surgery
Hossein Rabbani, Rahele Kafieh, Mahdi Kazemian Jahromi, Sahar Jorjandi, Alireza Mehri Dehnavi, Fedra Hajizadeh, Alireza Peyman
Optical Coherence Tomography (OCT) is one of the most informative methodologies in ophthalmology and provides cross sectional images from anterior and posterior segments of the eye. Corneal diseases can be diagnosed by these images and corneal thickness maps can also assist in the treatment and diagnosis. The need for automatic segmentation of cross sectional images is inevitable since manual segmentation is time consuming and imprecise. In this paper, segmentation methods such as Gaussian Mixture Model (GMM), Graph Cut, and Level Set are used for automatic segmentation of three clinically important corneal layer boundaries on OCT images...
2016: International Journal of Biomedical Imaging
Marcony R Santhiago, Natalia T Giacomin, David Smadja, Samir J Bechara
This review outlines risk factors of post-laser in situ keratomileusis (LASIK) ectasia that can be detected preoperatively and presents a new metric to be considered in the detection of ectasia risk. Relevant factors in refractive surgery screening include the analysis of intrinsic biomechanical properties (information obtained from corneal topography/tomography and patient's age), as well as the analysis of alterable biomechanical properties (information obtained from the amount of tissue altered by surgery and the remaining load-bearing tissue)...
2016: Clinical Ophthalmology
Yan Li, Winston Chamberlain, Ou Tan, Robert Brass, Jack L Weiss, David Huang
PURPOSE: To screen for subclinical keratoconus by analyzing corneal, epithelial, and stromal thickness map patterns with Fourier-domain optical coherence tomography (OCT). SETTING: Four centers in the United States. DESIGN: Cross-sectional observational study. METHODS: Eyes of normal subjects, subclinical keratoconus eyes, and the topographically normal eye of a unilateral keratoconus patient were studied. Corneas were scanned using a 26,000 Hz Fourier-domain OCT system (RTVue)...
February 2016: Journal of Cataract and Refractive Surgery
Raksha Urs, Harriet O Lloyd, Dan Z Reinstein, Ronald H Silverman
PURPOSE: To compare corneal thickness and epithelial thickness measurements in maps obtained using the RTVue spectral domain optical coherence tomography (SD-OCT) system and the Artemis 2 immersion arc-scanning very-high-frequency ultrasound (VHF-US) system. SETTING: Department of Ophthalmology, Columbia University Medical Center, New York, New York, USA. DESIGN: Comparative study. METHODS: Eyes of normal volunteers were scanned with the SD-OCT system followed by the VHF-US system and then again by the SD-OCT system...
January 2016: Journal of Cataract and Refractive Surgery
Pablo Zoroquiain, Maria Sb Ganimi, Sarah Alghamdi, Julia V Burnier, Sultan S Aldrees, Miguel N Burnier
Conjunctival melanoma is a rare malignant tumour of the eye. Its diagnosis represents a challenge for general pathologists due to low exposure to ocular biopsies and a broad differential diagnosis. In addition, conjunctival samples are often small and are associated with a high frequency of artefacts due to their processing. Here, we present the first case to date of a traumatic iridial extrusion masquerading as a conjunctival melanocytic neoplasm. An 83-year-old Asian man presented with a conjunctival-pigmented nodule surrounded by an area of diffuse pigmentation...
2016: Ecancermedicalscience
Nadia Zakaria, Sorcha Ní Dhubhghaill, Michiel Taal, Zwi Berneman, Carina Koppen, Marie-José Tassignon
PURPOSE: Cultivated limbal epithelial stem cell transplantation (CLET) is a surgical approach reserved for severe stem cell deficiency and anterior segment pathology. The recipient cornea typically displays a highly vascularized pannus that must be dissected before graft placement. Extensive pannus dissection, however, can result in corneal thinning and increase the risk of ectasia and ultimately, of perforation. We aimed to examine the value of anterior segment optical coherence tomography (AS-OCT) intraoperatively during pannus resection and long-term postoperative follow-up of CLET surgery...
November 2015: Asia-Pacific Journal of Ophthalmology
Dan Z Reinstein, Timothy J Archer, Raksha Urs, Marine Gobbe, Arindam RoyChoudhury, Ronald H Silverman
PURPOSE: To assess the effectiveness of a keratoconus-detection algorithm derived from Artemis very high-frequency (VHF) digital ultrasound (ArcScan Inc., Morrison, CO) epithelial thickness maps in the fellow eye from a series of patients with unilateral keratoconus. METHODS: The study included 10 patients with moderate to advanced keratoconus in one eye but a clinically and algorithmically topographically normal fellow eye. VHF digital ultrasound epithelial thickness data were acquired and a previously developed classification model was applied for identification of keratoconus to the clinically normal fellow eyes...
November 2015: Journal of Refractive Surgery
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