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Browning polyphenols

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https://www.readbyqxmd.com/read/29322691/raspberry-supplementation-improves-insulin-signaling-and-promotes-brown-like-adipocyte-development-in-white-adipose-tissue-of-obese-mice
#1
Tong Xing, Yifei Kang, Xinglian Xu, Bo Wang, Min Du, Mei-Jun Zhu
SCOPE: Excessive lipid accumulation in white adipose tissue (WAT) leads to chronic inflammation and metabolic dysfunction. Raspberry (RB) contains high amount of polyphenolics and dietary fibers. The objective of the study is to evaluate effects of RB supplementation on WAT morphology, inflammation and insulin signaling in high fat diet (HFD)-induced obese mice, and further explore the underlying mechanisms. METHODS AND RESULTS: C57BL/6J mice were fed with a control diet or a HFD supplemented with 0 or 5% freeze dried RB for 12 weeks...
January 10, 2018: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/29291143/comparative-study-of-the-banana-pulp-browning-process-of-giant-dwarf-and-fhia-23-during-fruit-ripening-based-on-image-analysis-and-the-polyphenol-oxidase-and-peroxidase-biochemical-properties
#2
Pilar Escalante-Minakata, Vrani Ibarra-Junquera, José de Jesús Ornelas-Paz, Victoria García-Ibáñez, José J Virgen-Ortíz, Apolinar González-Potes, Jaime D Pérez-Martínez, Mario Orozco-Santos
This work presents a novel method to associate the polyphenol oxidase (PPO) and the peroxidase (POD) activities with the ripening-mediated color changes in banana peel and pulp by computational image analysis. The method was used to follow up the de-greening of peel and browning of homogenized pulp from 'Giant Dwarf' (GD: Musa AAA, subgroup Cavendish) and FHIA-23 (tetraploid hybrid, AAAA) banana cultivars. In both cultivars, the color changes of peel during the ripening process clearly showed four stages, which were used to group the fruit into ripening stages...
January 2018: 3 Biotech
https://www.readbyqxmd.com/read/29287342/inhibitory-effects-of-edible-seaweeds-polyphenolics-and-alginates-on-the-activities-of-porcine-pancreatic-%C3%AE-amylase
#3
Nazikussabah Zaharudin, Armando Asunción Salmeán, Lars Ove Dragsted
Edible seaweeds are valuable because of their organoleptic properties and complex polysaccharide content. A study was conducted to investigate the potential of dried edible seaweed extracts, its potential phenolic compounds and alginates for α-amylase inhibitory effects. The kinetics of inhibition was assessed in comparison with acarbose. The methanol extract of Laminaria digitata and the acetone extract of Undaria pinnatifida showed inhibitory activity against α-amylase, IC50 0.74 ± 0.02 mg/ml and 0...
April 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29251618/-evaluation-of-adjuvant-effects-of-fucoidane-from-brown-seaweed-fucus-evanescens-and-its-structural-analogues-for-the-strengthening-vaccines-effectiveness
#4
T A Kuznetsova, L A Ivanushko, E V Persiyanova, A L Shutikova, S P Ermakova, M Yu Khotimchenko, N N Besednova
The use of sulfated polysaccharides from brown seaweed Fucus evanescens as adjuvants (native fucoidan in combination with polyphenols, fucoidan without polyphenols, a product of enzymatic hydrolysis of fucoidan) stimulated the formation of specific antibodies to the surface antigen of the hepatitis B virus (HBs-AG). Immunization of mice with vaccine compositions containing HBs-AG and fucoidan samples resulted in increasing the serum level of the pro-inflammatory (TNF-a, IFN-g, IL-2) cytokines. Increased production of these cytokines was detected in the culture of splenocytes additionally stimulated in vitro by fucoidans or phytohemagglutinin...
November 2017: Biomedit︠s︡inskai︠a︡ Khimii︠a︡
https://www.readbyqxmd.com/read/29243815/effect-of-grinding-at-modified-atmosphere-or-vacuum-on-browning-antioxidant-capacities-and-oxidative-enzyme-activities-of-apple
#5
Ah-Na Kim, Kyo-Yeon Lee, Hyun-Jin Kim, Jiyeon Chun, William L Kerr, Sung-Gil Choi
This study evaluated the effects of grinding at atmospheric pressure (control), under vacuum (∼2.67 kPa), or with modified atmosphere (N2 and CO2 ) on the browning, antioxidant activity, phenolics, and oxidative enzyme activity of apples as a function of time. The control group was affected most, showing distinct browning and losing most of the antioxidant activity and concentrations of the main phenolic compounds. The modified atmosphere groups retained color, antioxidant activity, and phenolic compounds better than the control group...
December 15, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/29241566/a-catechol-oxidase-acppo-from-cherimoya-annona-cherimola-mill-is-localized-to-the-golgi-apparatus
#6
Patricio Olmedo, Adrián A Moreno, Dayan Sanhueza, Iván Balic, Christian Silva-Sanzana, Baltasar Zepeda, Julian C Verdonk, César Arriagada, Claudio Meneses, Reinaldo Campos-Vargas
Cherimoya (Annona cherimola) is an exotic fruit with attractive organoleptic characteristics. However, it is highly perishable and susceptible to postharvest browning. In fresh fruit, browning is primarily caused by the polyphenol oxidase (PPO) enzyme catalyzing the oxidation of o-diphenols to quinones, which polymerize to form brown melanin pigment. There is no consensus in the literature regarding a specific role of PPO, and its subcellular localization in different plant species is mainly described within plastids...
January 2018: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/29218491/a-specific-amino-acid-residue-in-the-catalytic-site-of-dandelion-polyphenol-oxidases-acts-as-selector-for-substrate-specificity
#7
Sarah M Prexler, Ratna Singh, Bruno M Moerschbacher, Mareike E Dirks-Hofmeister
Successful site-directed mutagenesis combined with in silico modeling and docking studies for the first time offers experimental proof of the role of the 'substrate selector' residue in plant polyphenol oxidases. The plant and fungi enzymes responsible for tissue browning are called polyphenol oxidases (PPOs). In plants, PPOs often occur as families of isoenzymes which are differentially expressed, but little is known about their physiological roles or natural substrates. In a recent study that explored these structure-function relationships, the eleven known dandelion (Taraxacum officinale) PPOs were shown to separate into two different phylogenetic groups differing in catalytic cavity architecture, kinetic parameters, and substrate range...
December 7, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/29195964/phenolics-content-fruit-flesh-colour-and-browning-in-cultivated-eggplant-wild-relatives-and-interspecific-hybrids-and-implications-for-fruit-quality-breeding
#8
Prashant Kaushik, Pietro Gramazio, Santiago Vilanova, María D Raigón, Jaime Prohens, Mariola Plazas
Increasing the content in bioactive phenolics in the eggplant (Solanum melongena) fruit is of interest, but may result in enhanced browning. We evaluated six varieties of S. melongena, 22 accessions of wild related species and 42 interspecific hybrids between cultivated eggplant and wild relatives for phenolics content, fruit flesh colour, polyphenol oxidase (PPO) activity, and fruit flesh browning. Wild relatives generally had a higher content in phenolics and a broader range of variation than cultivated eggplant...
December 2017: Food Research International
https://www.readbyqxmd.com/read/29171232/-process-of-gallnut-suppository-preparation
#9
Mubarak Iminjan, Xue-Mei Cheng, Nazuk Abudu, Abdurahman Nurulla, Zhi-Hong Ji, Halmurat Upur
The main objective was to research the process of gallnut suppository preparation with its water extract as the main drug, and evaluate its irritation to rectal mucosa. gallnut extract was obtained by decocting method, and its suppository preparation was obtained by fusion method with semi-synthetic aliphatic esters and rose flower oil as the matrix. Weight difference and in vitro melting time limit of the suppository were assayed and UV-Vis was used to determine the contents of polyphenols, tannin and saccharide...
August 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29163391/colletotrichum-gloeosporioides-contaminated-tea-infusion-blocks-lipids-reduction-and-induces-kidney-damage-in-mice
#10
Jin Li, Kang Sun, Qingping Ma, Jin Chen, Le Wang, Dingjun Yang, Xuan Chen, Xinghui Li
When the homogenate of fresh tea tree leaves was fermented to produce black tea beverage, the Colletotrichum gloeosporioides (main pathogen or endophyte of Camellia sinensis) may be mixed into the fermentation liquor. However, it was unclear whether C. gloeosporioides-contaminated tea beverage would damage human health. Therefore, we investigated the changes of functional components and the influences on mice. C. gloeosporioides was added to the green tea infusion. After cultivation of 48 h, tea polyphenols, caffeine, and L-theanine decreased by 31...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29157606/high-performance-thin-layer-chromatography-hptlc-direct-bioautography-as-a-method-of-choice-for-alpha-amylase-and-antioxidant-activity-evaluation-in-marine-algae
#11
Snezana Agatonovic-Kustrin, David W Morton
High-Performance Thin-layer chromatography (HPTLC) combined with DPPH free radical method and α-amylase bioassay was used to compare antioxidant and antidiabetic activities in ethanol and ethyl acetate extracts from 10 marine macroalgae species (3 Chlorophyta, 4 Phaeophyta and 3 Rhodophyta) from Blue Lagoon beach (Malaysia). Samples were also evaluated for their phenolic and stigmasterol content. On average, higher antioxidant activity was observed in the ethyl acetate extracts (55.1mg/100g gallic acid equivalents (GAE) compared to 35...
November 14, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/29129902/quantification-of-fraglide-1-a-new-functional-ingredient-in-vinegars
#12
Aydan H Yatmaz, Tetsuaki Kinoshita, Akio Miyazato, Masahiro Takagi, Yoshio Tsujino, Fumiaki Beppu, Naohiro Gotoh
Vinegar is a widely used condiment in the world, and is produced from ethanol by acetic acid fermentation. Different fruits, vegetables, cereals, and wines can be used as ingredients for vinegar production. It is known that vinegar has many nutrient components such as organic acids, polyphenols, and aromatic compounds. Because of these bioactive components, it has many health benefits. China has a long history of producing vinegar and has been using it for health products and as medicine. Chinese aromatic Zhenjiang vinegar (Kozu) is produced from sticky rice...
November 13, 2017: Journal of Oleo Science
https://www.readbyqxmd.com/read/29125293/oxidation-of-wine-polyphenols-by-secretomes-of-wild-botrytis-cinerea-strains-from-white-and-red-grape-varieties-and-determination-of-their-specific-laccase-activity
#13
Sabrina Zimdars, Julia Hitschler, Andreas Schieber, Fabian Weber
Processing of Botrytis cinerea infected grapes leads to enhanced enzymatic browning reactions mainly caused by the enzyme laccase which is able to oxidize a wide range of phenolic compounds. The extent of color deterioration depends on the activity of the enzymes secreted by the fungus. The present study revealed significant differences in the oxidative properties of secretomes of several B. cinerea strains isolated from five grape varieties. The presumably laccase containing secretomes varied in their catalytic activity toward six phenolic compounds present in grapes...
November 10, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29125219/effects-of-a-grapevine-shoot-extract-containing-resveratrol-and-resveratrol-oligomers-on-intestinal-adenoma-development-in-mice-in-vitro-and-in-vivo-studies
#14
Michael T Empl, Hong Cai, Shan Wang, Johannes Junginger, Tina Kostka, Marion Hewicker-Trautwein, Karen Brown, Andreas J Gescher, Pablo Steinberg
SCOPE: Evidence suggests that the dietary consumption of plant extracts containing polyphenols might help prevent the onset of cancers of the gastrointestinal tract. In the present study, the chemopreventive and antiproliferative efficacy of a grapevine shoot extract (Vineatrol(®) 30) containing resveratrol and resveratrol oligomers was investigated in vivo and in vitro. METHODS AND RESULTS: The in vivo study was performed using Apc(Min) mice on a high-fat diet, which represents a model of human adenomatous polyposis, while the potential of the extract as well as some of its isolated constituents to inhibit intestinal adenoma cell proliferation in vitro was investigated using APC10...
November 10, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/29061705/polyphenol-and-caffeine-rich-post-fermented-pu-er-tea-improves-diet-induced-metabolic-syndrome-by-remodeling-intestinal-homeostasis-in-mice
#15
Xiaoyu Gao, Qiuhong Xie, Ping Kong, Ling Liu, Sheng Sun, Boyu Xiong, Baojia Huang, Liang Yan, Jun Sheng, Hongyu Xiang
Post-fermented Pu-er tea (PE) protects against metabolic syndrome (MS), but little is known regarding its underlying mechanisms. Animal experiments were performed to determine whether gut microbiota mediated the improvement in diet-induced MS by PE and its main active components (PEAC). We confirmed that PE altered the body composition and energy efficiency; attenuated metabolic endotoxemia and systemic and multiple-tissue inflammation; and improved the glucose and lipid metabolism disorder in high-fat diet (HFD)-fed mice via multiple pathways...
October 23, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/29055247/optimization-and-validation-of-reverse-phase-hplc-method-for-qualitative-and-quantitative-assessment-of-polyphenols-in-seaweed
#16
Gaurav Rajauria
A simple reverse phase-high performance liquid chromatography (RP-HPLC) coupled to a diode array detector (DAD) and negative ion electrospray mass spectrometer (ESI-MS) method was developed for simultaneous identification and quantification of phenolic antioxidants in seaweed. The proposed method was validated in terms of linearity, limits of detection (LOD), limits of quantification (LOQ), recovery and intermediate precision. The calibration curves were linear with correlation coefficient ranging from 0.9909 to 0...
October 13, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29027275/multifaceted-supramolecular-interactions-from-c-methylresorcin-4-arene-lead-to-an-enhancement-in-in-vitro-antibacterial-activity-of-gatifloxacin
#17
Arnab Dawn, Harish Chandra, Chandra Ade-Browne, Jagjit Yadav, Harshita Kumari
Mimicking the antibacterial activity of polyphenols in synthetic systems is an attractive approach for the development of novel active pharmaceutical ingredients. Resorcinarenes represent a class of polyphenols which have been exploited for decades for their attractive chemical scaffold suitable for forming host-guest complexes with hydrophobic guest molecules. However, the polyphenolic character of resorcinarenes, which could be a potential asset to the pharmaceutical industry, have been least exploited. The present work represents an unprecedented interplay of chemical nature of resorcinarene conferring an antimicrobial activity together with its ability to interact with an antibacterial drug gatifloxacin, improving the overall antibacterial activity...
October 12, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/29017399/recombinant-expression-purification-and-characterization-of-polyphenol-oxidase-2-vvppo2-from-shine-muscat-vitis-labruscana-bailey-%C3%A3-vitis-vinifera-l
#18
Ayako Katayama-Ikegami, Yuka Suehiro, Takane Katayama, Kazushi Jindo, Hiroyuki Itamura, Tomoya Esumi
Polyphenol oxidases (PPOs) catalyze browning reactions in various plant organs, therefore controlling the reactions is important for the food industry. PPOs have been assumed to be involved in skin browning of white grape cultivars; however, the molecular mechanism underlying PPO-mediated browning process remains elusive. We have recently identified a new PPO gene named VvPPO2 from "Shine Muscat" (Vitis labruscana Bailey × V. vinifera L.), and have shown that the gene is transcribed at a higher level than the previously identified VvPPO1 in browning, physiologically disordered berry skins at the maturation stage...
October 11, 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/28974022/effect-of-green-and-brown-propolis-extracts-on-the-expression-levels-of-micrornas-mrnas-and-proteins-related-to-oxidative-stress-and-inflammation
#19
Vincenzo Zaccaria, Valeria Curti, Arianna Di Lorenzo, Alessandra Baldi, Cristina Maccario, Sabrina Sommatis, Roberto Mocchi, Maria Daglia
A large body of evidence highlights that propolis exerts many biological functions that can be ascribed to its antioxidant and anti-inflammatory components, including different polyphenol classes. Nevertheless, the molecular mechanisms are yet unknown. The aim of this study is to investigate the mechanisms at the basis of propolis anti-inflammatory and antioxidant activities. The effects of two brown and green propolis extracts-chemically characterized by RP-HPLC-PDA-ESI-MSn-on the expression levels of miRNAs associated with inflammatory responses (miR-19a-3p and miR-203a-3p) and oxidative stress (miR-27a-3p and miR-17-3p), were determined in human keratinocyte HaCat cell lines, treated with non-cytotoxic concentrations...
October 1, 2017: Nutrients
https://www.readbyqxmd.com/read/28958519/full-inhibition-of-enzymatic-browning-in-the-presence-of-thiol-functionalised-silica-nanomaterial
#20
Sara Muñoz-Pina, José V Ros-Lis, Ángel Argüelles, Carmen Coll, Ramón Martínez-Máñez, Ana Andrés
Darkening processed fruits and vegetables is caused mainly by enzymatic browning through polyphenol oxidase (PPO) action. Accordingly, we explored the potential of four silica-based materials (MCM-41 nanometric size, MCM-41 micrometric size, UVM-7 and aerosil), non-functionalised and functionalised with thiol groups, to inhibit PPO activity in the model system and apple juice. All materials showed relevant performance when immobilising and inhibiting PPO in model systems, and support topology is a main factor for enzyme immobilisation and inhibition...
February 15, 2018: Food Chemistry
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