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Mayra Diosa-Toro, Liliana Echavarría-Consuegra, Jacky Flipse, Geysson Javier Fernández, Joost Kluiver, Anke van den Berg, Silvio Urcuqui-Inchima, Jolanda M Smit
BACKGROUND: Due to the high burden of dengue disease worldwide, a better understanding of the interactions between dengue virus (DENV) and its human host cells is of the utmost importance. Although microRNAs modulate the outcome of several viral infections, their contribution to DENV replication is poorly understood. METHODS AND PRINCIPAL FINDINGS: We investigated the microRNA expression profile of primary human macrophages challenged with DENV and deciphered the contribution of microRNAs to infection...
October 2017: PLoS Neglected Tropical Diseases
Jacky Flipse, Mayra A Diosa-Toro, Tabitha E Hoornweg, Denise P I van de Pol, Silvio Urcuqui-Inchima, Jolanda M Smit
The dogma is that the human immune system protects us against pathogens. Yet, several viruses, like dengue virus, antagonize the hosts' antibodies to enhance their viral load and disease severity; a phenomenon called antibody-dependent enhancement of infection. This study offers novel insights in the molecular mechanism of antibody-mediated enhancement (ADE) of dengue virus infection in primary human macrophages. No differences were observed in the number of bound and internalized DENV particles following infection in the absence and presence of enhancing concentrations of antibodies...
2016: Scientific Reports
Jacky Flipse, Silvia Torres, Mayra Diosa-Toro, Heidi van der Ende-Metselaar, José Herrera-Rodriguez, Silvio Urcuqui-Inchima, Anke Huckriede, Izabela A Rodenhuis-Zybert, Jolanda M Smit
Dengue virus infects immune cells, including monocytes, macrophages and dendritic cells (DC). We compared virus infectivity in macrophages and DC, and found that the virus origin determined the cell tropism of progeny virus. The highest efficiency of re-infection was seen for macrophage-derived dengue virus. Furthermore, in the presence of enhancing antibodies, macrophage-derived virus gave greater enhancement of infection compared with immature DC-derived virus. Taken together, our results highlight the importance of macrophages in dengue infection...
July 2016: Journal of General Virology
Jorge Andrés Castillo, Juan Camilo Castrillón, Mayra Diosa-Toro, Juan Guillermo Betancur, Georges St Laurent, Jolanda M Smit, Silvio Urcuqui-Inchima
BACKGROUND: Dengue virus (DENV) is the most common vector-borne viral infection worldwide with approximately 390 million cases and 25,000 reported deaths each year. MicroRNAs (miRNAs) are small non-coding RNA molecules responsible for the regulation of gene expression by repressing mRNA translation or inducing mRNA degradation. Although miRNAs possess antiviral activity against many mammalian-infecting viruses, their involvement in DENV replication is poorly understood. METHODS: Here, we explored the relationship between DENV and cellular microRNAs using bioinformatics tools...
January 27, 2016: BMC Infectious Diseases
Daniel Arango, Mayra Diosa-Toro, Laura S Rojas-Hernandez, Jessica L Cooperstone, Steven J Schwartz, Xiaokui Mo, Jinmai Jiang, Thomas D Schmittgen, Andrea I Doseff
SCOPE: High incidence of inflammatory diseases afflicts the increasing aging-population infringing a great health burden. Dietary flavonoids, including the flavone apigenin, are emerging as important anti-inflammatory nutraceuticals due to their health benefits, lack of adverse effects and reduced costs. MicroRNAs (miRs) play a central role in inflammation by regulating gene expression, yet how dietary ingredients affect miRs is poorly understood. The aim of this study was to identify miRs involved in the anti-inflammatory activity of apigenin and apigenin-rich diets and determine their immune regulatory mechanisms in macrophages and in vivo...
April 2015: Molecular Nutrition & Food Research
Mayra Diosa-Toro, Silvio Urcuqui-Inchima, Jolanda M Smit
Flaviviruses are the most prevalent arthropod-borne viruses worldwide, and nearly half of the 70 Flavivirus members identified are human pathogens. Despite the huge clinical impact of flaviviruses, there is no specific human antiviral therapy available to treat infection with any of the flaviviruses. Therefore, there is a continued search for novel therapies, and this review describes the current knowledge on the usage of RNA interference (RNAi) in combating flavivirus infections. RNAi is a process of sequence-specific gene silencing triggered by double-stranded RNA...
2013: Advances in Virus Research
Gregory Hostetler, Ken Riedl, Horacio Cardenas, Mayra Diosa-Toro, Daniel Arango, Steven Schwartz, Andrea I Doseff
SCOPE: Flavones have reported anti-inflammatory activities, but the ability of flavone-rich foods to reduce inflammation is unclear. Here, we report the effect of flavone glycosylation in the regulation of inflammatory mediators in vitro and the absorption of dietary flavones in vivo. METHODS AND RESULTS: The anti-inflammatory activities of celery extracts, some rich in flavone aglycones and others rich in flavone glycosides, were tested on the inflammatory mediators tumor necrosis factor α (TNF-α) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) in lipopolysaccharide-stimulated macrophages...
April 2012: Molecular Nutrition & Food Research
Mayra A Diosa-Toro, Fabián A Jaimes B, María T Rugeles L, Paula A Velilla H
Sepsis, defined as a systemic inflammatory response syndrome caused by an infection, is a significant cause of mortality worldwide. It is currently accepted that death associated to sepsis is due to an immune hyperactivation state involving the development of a broad proinflammatory response along with alterations in the coagulation system. It is now clear that besides the inflammatory events, the clinical course of sepsis is characterized by the development of an anti-inflammatory response that could lead to death in its attempt to balance the initial response...
December 2011: Revista Chilena de Infectología: órgano Oficial de la Sociedad Chilena de Infectología
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