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filmarray respiratory

Frederick S Nolte, Lori Gauld, Susan B Barrett
We compared two rapid, point-of care, nucleic acid amplification tests for detection of influenza A and B viruses (Alere™ i, Alere and COBAS: ® Liat, Roche Diagnostics) with the influenza A and B virus test components of FilmArray® respiratory panel (BioFire Diagnostics) using 129 respiratory specimens collected in universal viral transport medium (80 influenza A and 16 influenza B virus positive) from both adult and pediatric patients. The sensitivities of the Alere test were 71.3% for influenza A and 93...
August 31, 2016: Journal of Clinical Microbiology
David Gilbert, Gita Gelfer, Lian Wang, Jillian Myers, Kristina Bajema, Michael Johnston, James Leggett
Two diagnostic bundles were compared in 127 evaluable patients admitted with community-acquired pneumonia (CAP). Diagnostic modalities in all patients included cultures of sputum (if obtainable) and blood, urine for detection of the antigens of Streptococcus pneumoniae and Legionella pneumophila, and nasal swabs for PCR probes for S. pneumoniae and Staphylococcus aureus. At least one procalcitonin level was measured in all patients. For virus detection, patients were randomized to either a 5-virus, lab-generated PCR panel or the broader and faster FilmArray PCR panel...
September 2016: Diagnostic Microbiology and Infectious Disease
Urania Rappo, Audrey N Schuetz, Stephen G Jenkins, David P Calfee, Thomas J Walsh, Martin T Wells, James P Hollenberg, Marshall J Glesby
Rapid and definitive diagnosis of viral respiratory infections is imperative in patient triage and management. We compared the outcomes for adult patients with positive tests for respiratory viruses at a tertiary care center across two consecutive influenza seasons (winters of 2010-2011 and 2012). Infections were diagnosed by conventional methods in the first season and by multiplex PCR (FilmArray) in the second season. FilmArray decreased the time to diagnosis of influenza compared to conventional methods (median turnaround times of 1...
August 2016: Journal of Clinical Microbiology
Jonathan H K Chen, Ho-Yin Lam, Cyril C Y Yip, Sally C Y Wong, Jasper F W Chan, Edmond S K Ma, Vincent C C Cheng, Bone S F Tang, Kwok-Yung Yuen
A broad range of viral and bacterial pathogens can cause acute respiratory tract infection. For rapid detection of a broad respiratory pathogen spectrum, multiplex real-time PCR is ideal. This study evaluated the performance of the new Luminex NxTAG Respiratory Pathogen Panel (NxTAG-RPP) in comparison with the BioFire FilmArray Respiratory Panel (FA-RP) or singleplex real-time PCR as reference. A total of 284 clinical respiratory specimens and 3 influenza A/H7N9 viral culture samples were tested. All clinical specimens were processed and analyzed in parallel using NxTAG-RPP and the reference standard method...
July 2016: Journal of Clinical Microbiology
X Qin, D M Zerr, M P Kronman, A L Adler, J E Berry, S Rich, A M Buccat, M Xu, J A Englund
A state-wide pertussis outbreak occurred in Washington during the winter-spring months of 2012, concurrent with respiratory viral season. We compared performance characteristics of a laboratory-developed pertussis PCR (LD-PCR for Bordetella pertussis, Bordetella parapertussis, and Bordetella holmesii) and rapid multiplex PCR (RM-PCR) for respiratory viruses (FilmArray™, BioFire, B. pertussis data unblinded following FDA approval post outbreak). We analyzed three cohorts of patients using physician testing orders as a proxy for clinical suspicion for pertussis or respiratory viruses: Cohort 1, tested by LD-PCR for pertussis pathogens only by nasopharyngeal swab; Cohort 2, by RM-PCR for respiratory viruses only by mid-nasal turbinate swab; and Cohort 3, by both methods...
2016: Annals of Clinical Microbiology and Antimicrobials
Mark G Wahrenbrock, Scott Matushek, Sue Boonlayangoor, Vera Tesic, Kathleen G Beavis, Angella Charnot-Katsikas
The Xpert Flu/RSV XC was compared to the FilmArray respiratory panel for detection of influenza (Flu) A, Flu B, and respiratory syncytial virus (RSV), using 128 nasopharyngeal swabs. Positive agreements were 100% for Flu A and RSV and 92.3% for Flu B. The Xpert may be useful in clinical situations when extensive testing is not required and may serve an important role in laboratories already performing broader respiratory panel testing.
July 2016: Journal of Clinical Microbiology
Eunkyung Song, Huanyu Wang, Doug Salamon, Preeti Jaggi, Amy Leber
The FilmArray Respiratory Panel (RP) v1.7 assay has improved sensitivity for detection of human adenovirus (HAdV), compared to an earlier version (RP v1.6). RP v1.7 was designed for detection of species B, C, and E but may show variable detection of species A, D, and F. We sought to evaluate the clinical and analytical performance of RP v1.7 for detection of HAdV in a large pediatric cohort. Respiratory specimens obtained from a tertiary care children's hospital between February 2014 and February 2015 were tested for HAdV by RP v1...
June 2016: Journal of Clinical Microbiology
M Dorsch, B Meehan, W P Michalski, H G Heine, A J Foord, G Carlile, J Wang, S McCullough, K Zuelke
In this study, we explored the potential utility of the human-focused FilmArray® Respiratory Panel for the diagnosis of a broad range of influenza viruses of veterinary concern as compared with the standard portfolio of recommended TaqMan®-based diagnostic tests. In addition, we discuss some potential operational advantages associated with the use of such integrated sample extraction, amplification and analysis devices in the context of a future long-term, dual-role strategy for the detection of emergency diseases of both human and veterinary concern...
March 2016: Australian Veterinary Journal
Dena R Shibib, Scott M Matushek, Kathleen G Beavis, Susan H Gawel, Angella Charnot-Katsikas
During the enterovirus D68 (EV-D68) outbreak of 2014, the BioFire FilmArray (FA) respiratory panel was used to detect rhinovirus/enterovirus in respiratory specimens; suspected EV-D68-positive specimens were sent to CDC for confirmation. Positive rhinovirus/enterovirus FA targets revealed patterns loosely associated with EV-D68 that may be useful for confirmation triaging.
February 2016: Journal of Clinical Microbiology
Natalya Azadeh, Kenneth K Sakata, Anjuli M Brighton, Holenarasipur R Vikram, Thomas E Grys
The FilmArray respiratory panel (FARP) reliably and rapidly identifies 17 viruses and 3 bacterial pathogens. A nasopharyngeal swab FARP (NP FARP) is performed for many patients with respiratory symptoms. For patients who are acutely ill or immunocompromised or fail to improve, a bronchoalveolar lavage sample FARP (BAL FARP) is performed in addition to the NP FARP. To date, no studies have compared the yield of a BAL FARP with that of an NP FARP. We retrospectively studied all patients who had a BAL FARP within 7 days after an NP FARP between June 2013 and May 2014...
December 2015: Journal of Clinical Microbiology
Gita Gelfer, James Leggett, Jillian Myers, Luan Wang, David N Gilbert
The etiology of community-acquired pneumonia (CAP) is determined in less than half of the patients based on cultures of sputum and blood plus testing urine for the antigens of Streptococcus pneumoniae and Legionella pneumophila. This study added nasal polymerase chain reaction (PCR) probes for S. pneumoniae, Staphylococcus aureus, and respiratory viruses. Serum procalcitonin (PCT) levels were measured. Pathogens were identified in 78% of the patients. For detection of viruses, patients were randomized to either a 5-virus laboratory-generated PCR bundle or the 17-virus FilmArray PCR platform...
December 2015: Diagnostic Microbiology and Infectious Disease
Carrie L Byington, Krow Ampofo, Chris Stockmann, Frederick R Adler, Amy Herbener, Trent Miller, Xiaoming Sheng, Anne J Blaschke, Robert Crisp, Andrew T Pavia
BACKGROUND: This study: (1) describes the viral etiology of respiratory illness by prospectively collecting weekly symptom diaries and nasal swabs from families for 1 year, (2) analyzed data by reported symptoms, virus, age, and family composition, and (3) evaluated the duration of virus detection. METHODS: Twenty-six households (108 individuals) provided concurrent symptom and nasal swab data for 4166 person-weeks. The FilmArray polymerase chain reaction (PCR) platform (BioFire Diagnostics, LLC) was used to detect 16 respiratory viruses...
October 15, 2015: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
Richard E Nelson, Chris Stockmann, Adam L Hersh, Andrew T Pavia, Kent Korgenksi, Judy A Daly, Marc R Couturier, Krow Ampofo, Emily A Thorell, Elizabeth H Doby, Jeff A Robison, Anne J Blaschke
BACKGROUND: Rapid multiplex polymerase chain reaction (PCR) assays simultaneously detect several respiratory viral pathogens with high sensitivity. Maximizing detection of influenza at the point of care has the potential to reduce unnecessary antibiotic use, laboratory tests and hospitalizations. However, the cost-effectiveness of rapid multiplex PCR assays for influenza has not been compared with other diagnostic methods in children. METHODS: For children presenting to the emergency department with influenza-like illness, we compared costs and outcomes using 4 different testing strategies for detection of influenza: (1) a rapid multiplex PCR platform (FilmArray); (2) traditional PCR; (3) direct-fluorescent antibody and (4) rapid antigen tests...
June 2015: Pediatric Infectious Disease Journal
Jian Zhuge, Eric Vail, Jeffrey L Bush, Lauren Singelakis, Weihua Huang, Sheila M Nolan, Janet P Haas, Helen Engel, Millicent Della Posta, Esther C Yoon, John T Fallon, Guiqing Wang
An outbreak of severe respiratory illness associated with enterovirus D68 (EV-D68) infection was reported in mid-August 2014 in the United States. In this study, we evaluated the diagnostic utility of an EV-D68-specific real-time reverse transcription-PCR (rRT-PCR) that was recently developed by the Centers for Disease Control and Prevention in clinical samples. Nasopharyngeal (NP) swab specimens from patients in a recent outbreak of respiratory illness in the lower Hudson Valley, New York State, were collected and examined for the presence of human rhinovirus or enterovirus using the FilmArray Respiratory Panel (RP) assay...
June 2015: Journal of Clinical Microbiology
Robert C Jerris, Sally R Williams, Heather J MacDonald, Danielle R Ingebrigtsen, Lars F Westblade, Beverly B Rogers
BACKGROUND: The FilmArray Respiratory Panel (RP) detects multiple pathogens, including Bordetella pertussis. The multiplex PCR system is appropriate for a core laboratory or point of care due to ease of use. The purpose of this study is to compare the analytical sensitivity of the FilmArray RP, which targets the promoter region of the B. pertussis toxin gene, with the Focus real-time PCR assay, which targets the insertion sequence IS481. METHODS: Seventy-one specimens from patients aged 1 month to 18 years, which had tested positive for B...
May 2015: Journal of Clinical Pathology
Débora N Marcone, Guadalupe Carballal, Carmen Ricarte, Marcela Echavarria
Acute respiratory infections, which are commonly caused by viruses, are an important cause of morbidity and mortality in children. In Argentina, national surveillance programs for the detection of respiratory viruses are usually performed by using immunofluorescence (IF) assays, although it is well known that molecular methods are more sensitive. An automated multiplex PCR device, the FilmArray-Respiratory Panel (FilmArray-RP), can detect 17 viral and 3 bacterial pathogens in a closed system that requires only 5 min of hands-on time and 1h of instrumentation time...
January 2015: Revista Argentina de Microbiología
Romain Basmaci, Stéphane Bonacorsi, Brice Ilharreborde, Catherine Doit, Mathie Lorrot, Mahmoud Kahil, Benoît Visseaux, Nadhira Houhou, Philippe Bidet
AIM: Kingella kingae osteoarticular (KKO) infections are frequently associated with upper respiratory tract infections. However, no comparative studies detecting respiratory viruses had ever been performed between KKO and non-KKO (NKO). PATIENTS & METHODS: Eighteen viruses were searched by FilmArray(®) Respiratory Panel (BioFire Diagnostics, UT, USA) in the oropharynx of 6-to-48-month-children admitted for KKO and NKO in 2013. RESULTS: At least one virus was detected in the oropharynx of 19/21 (90...
2015: Future Microbiology
Emily K Martin, Jane Kuypers, Helen Y Chu, Kirsten Lacombe, Xuan Qin, Bonnie Strelitz, Miranda Bradford, Charla Jones, Eileen J Klein, Janet A Englund
BACKGROUND: Human rhinovirus (HRV) infections are highly prevalent, genetically diverse, and associated with both mild upper respiratory tract and more severe lower tract illnesses (LRTI). OBJECTIVE: To characterize the molecular epidemiology of HRV infections in young children seeking acute medical care. STUDY DESIGN: Nasal swabs collected from symptomatic children <3 years of age receiving care in the Emergency and Urgent Care Departments at Seattle Children's Hospital were analyzed by a rapid polymerase chain reaction (PCR) system (FilmArray(®)) for multiple viruses including HRV/enterovirus...
January 2015: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
Maria E Andersson, Sigvard Olofsson, Magnus Lindh
Recently, molecular methods capable of detecting almost all microbial agents that may cause acute respiratory infection have been introduced. The FilmArray Respiratory Panel assay, which integrates nucleic acid extraction, nested amplification and detection in a reaction pouch preloaded with all reagents required for detection of 17 viruses and 3 bacteria, was compared with an in-house real-time PCR that detects these agents in 8 parallel amplifications. When 128 clinical samples representing 18 of these agents were analysed by both assays the agreement was excellent, with kappa values ranging between 0...
December 2014: Scandinavian Journal of Infectious Diseases
S A Butt, V P Maceira, M E McCallen, K A Stellrecht
BACKGROUND: Due to the insensitivity of rapid tests for respiratory viruses, nucleic acid amplification tests are quickly becoming the standard of care. OBJECTIVES AND STUDY DESIGN: The performance of the FilmArray Respiratory Panel (RP) and Verigene RV+ (RV+) were compared in a retrospective analysis of 89 clinical specimens previously determined to be positive for the following viruses by our test of record, Prodesse (Pro): influenza A (29, FluA), influenza B (13, FluB), respiratory syncytial virus (12, RSV), human metapneumovirus (10, hMPV), parainfluenza (14, PIV), and adenovirus (10, AdV)...
November 2014: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
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