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Liposomes characterization

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https://www.readbyqxmd.com/read/28921442/preparation-of-immunoliposomes-by-direct-coupling-of-antibodies-based-on-a-thioether-bond
#1
Raquel Petrilli, Josimar O Eloy, Robert J Lee, Renata F V Lopez
Drug delivery is of paramount importance, since the drug needs to be delivered to a specific site, in adequate concentration, avoiding degradation in order to provide therapeutic efficacy. Different nanocarriers have been used over the years for this purpose and liposomes are well-established systems due to the high biocompatibility and the possibility to vehiculate both hydrophilic and lipophilic drugs. In order to circumvent the rapid clearance by the reticuloendothelial system and to avoid the healthy cells exposure to the drug, long circulating liposomes containing polyethyleneglycol (PEG) and functionalized liposomes for targeted delivery have been developed...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28918188/the-effect-of-thiolated-phospholipids-on-formation-of-supported-lipid-bilayers-on-gold-substrates-investigated-by-surface-sensitive-methods
#2
Abdulhalim Kılıç, M Fazeli Jadidi, Hakan Özgür Özer, Fatma Neşe Kök
Most of the model lipid membrane studies on gold involve the usage of various surface-modification strategies to rupture liposomes and induce lipid bilayer formation since liposomes with polar surfaces do not interact with bare, hydrophobic gold. In this study, a thiol-modified phospholipid, 1,2-Dipalmitoyl-sn-Glycero-3-Phosphothioethanol (DPPTE) was incorporated into phosphatidylcholine (PC) based liposomes to form supported lipid bilayer (SLB) on gold surfaces without further modification. The binding kinetics of liposomes with different DPPTE ratio (0...
September 8, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28917937/pyrazole-based-compounds-in-chitosan-liposomal-emulsion-for-antimicrobial-cotton-fabrics
#3
Ahmed Nada, Marwa Al-Moghazy, Ahmed A F Soliman, Gehan M T Rashwan, Taghreed Hosny Ahmed Eldawy, Ashraf Abd Elhakim Hasan, Galal Hosni Sayed
The chemistry of pyrazoles has gained increasing attention due to its diverse pharmacological properties such as antiviral, antagonist, antimicrobial, anticancer, anti-inflammatory, analgesic, anti-prostate cancer, herbicidal, acaricidal and insecticidal activities. 1-phenyl pyrazole-3, 5-diamine, 4-[2-(4-methylphenyl) diazenyl] and 1H- pyrazole-3 (1), 5-diamine, 4-[2-(4-methylphenyl) diazenyl] (2) were synthesized, characterized and encapsulated into liposomal chitosan emulsions for textile finishing. The chemical modifications of cotton fabrics were demonstrated by infrared analysis...
September 13, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28914064/-targeted-drug-delivery-system-potential-application-to-resveratrol
#4
Hassan Farghali, Ludmila Kameníková
Drug delivery system (DDS) is intended to increasing effectiveness of drugs through targeted distribution and to reducing of unwanted effects. In this mini-review, the basic principles of nanotechnology that were developed for DDS were reported including sections on the present research in key areas that are important for future investigations. Attention is paid on resveratrol as a model phytochemical with interesting pharmacologic profile which was demonstrated in great numbers of studies and for its wide use as supplemental therapy...
2017: Ceská a Slovenská Farmacie
https://www.readbyqxmd.com/read/28912139/a-combination-rnai-chemotherapy-layer-by-layer-nanoparticle-for-systemic-targeting-of-kras-p53-with-cisplatin-to-treat-non-small-cell-lung-cancer
#5
Li Gu, Jason Z Deng, Sweta Roy, Paula T Hammond
PURPOSE: Mutation of the Kirsten ras sarcoma viral oncogene homolog (KRAS) and loss of p53 function are commonly seen in non-small cell lung cancer (NSCLC). Combining therapeutics targeting these tumor defensive pathways with cisplatin in a single nanoparticle platform are rarely developed in clinic. EXPERIMENTAL DESIGN: Cisplatin was encapsulated in liposomes which multiple polyelectrolyte layers including siKRAS and miR-34a were built on to generate multifunctional layer-by-layer nanoparticle...
September 14, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/28903625/radioprotective-activity-of-curcumin-encapsulated-liposomes-against-genotoxicity-caused-by-gamma-cobalt-60-irradiation-in-human-blood-cells
#6
Minh-Hiep Nguyen, Pham Ngoc Duy, Bingxue Dong, Thi-Huynh-Nga Nguyen, Chi-Bao Bui, Kunn Hadinoto
PURPOSE: While the radioprotective activity of curcumin against genotoxicity has been well established, its poor oral bioavailability has limited its successful clinical applications. Nanoscale formulations, including liposomes, have been demonstrated to improve curcumin bioavailability. The objective of the present work was (1) to prepare and characterize curcumin-encapsulated liposomes (i.e. size, colloidal stability, encapsulation efficiency, and payload), and (2) subsequently to evaluate their radioprotective activity against genotoxicity in human blood cells caused by Gamma Cobalt-60 irradiation...
September 14, 2017: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/28898610/-visceral-leishmaniasis-in-hiv-infected-patients-the-challenge-of-relapse-and-treatment-failure
#7
Patrícia Cipriano, Ana Cláudia Miranda, Isabel Antunes, Kamal Mansinho
INTRODUCTION: Visceral leishmaniasis is an endemic disseminated infection, considered to be the third most frequent opportunistic parasitic infection in Europe. It is especially prevalent in patients co-infected with human immunodeficiency virus, in whom it poses a great therapeutic challenge due to increased risk of relapse. The goal of this study is to characterize a population of co-infected patients, as well as the efficiency of the adopted treatment strategies. MATERIAL AND METHODS: Retrospective study with a sample composed of all patients with visceral leishmaniasis and human immunodeficiency virus admitted in an Infectious Diseases ward over a period of 10 years...
June 30, 2017: Acta Médica Portuguesa
https://www.readbyqxmd.com/read/28887231/therapeutic-activity-of-superoxide-dismutase-containing-enzymosomes-on-rat-liver-ischaemia-reperfusion-injury-followed-by-magnetic-resonance-microscopy
#8
Paulo Marcelino, H Susana Marinho, Maria Celeste Campos, Ana Rita Neves, Carla Real, Filipa S Fontes, Alexandra Carvalho, Gabriel Feio, M Bárbara F Martins, M Luísa Corvo
Liver ischaemia-reperfusion injury (IRI) may occur during hepatic surgery and is unavoidable in liver transplantation. Superoxide dismutase enzymosomes (SOD-enzymosomes), liposomes where SOD is at the liposomal surface expressing enzymatic activity in intact form without the need of liposomal disruption, were developed with the aim of having a better insight into its antioxidant therapeutic outcome in IRI. We also aimed at validating magnetic resonance microscopy (MRM) at 7T as a tool to follow IRI. SOD-enzymosomes were characterized and tested in a rat ischaemia-reperfusion model and the therapeutic outcome was compared with conventional long circulating SOD liposomes and free SOD using biochemical liver injury biomarkers, histology and MRM...
September 6, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28877444/new-tethered-phospholipid-bilayers-integrating-functional-g-protein-coupled-receptor-membrane-protein
#9
Meriem Chadli, Samuel Rebaud, Ofelia Maniti, Bruno Tillier, Sandra Cortès, Agnes Girard-Egrot
Membrane proteins exhibiting extra- and intra-cellular domains require an adequate near-native lipid platform for their functional reconstitution. With this aim, we developed a new technology enabling the formation of a peptide-tethered Bilayer Lipid Membrane (pep-tBLM), a lipid bilayer grafted onto peptide spacers, by the way of a metal-chelate interaction. To this end, we designed an original peptide spacer derived from the natural α-laminin thiopeptide (P19), possessing a cysteine residue in N-terminal extremity for grafting on gold and modified in C-terminal extremity by four histidine residues (P19-4H)...
September 6, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28872688/liposomal-%C3%AE-galactosylceramide-is-taken-up-by-gut-associated-lymphoid-tissue-and-stimulates-local-and-systemic-immune-responses
#10
Kan Kaneko, Arlene McDowell, Yasuyuki Ishii, Sarah Hook
OBJECTIVES: α-Galactosylceramide (α-GalCer), a synthetic glycosphingolipid that exhibits potent immunostimulatory effects through activation of natural killer T (NKT) cells, can be used to treat conditions such as atopy, cancer, infection and autoimmunity. Administration of therapeutics through the oral route has advantages such as patient convenience, safety and reduced cost; however, there has been little research to investigate whether oral delivery of α-GalCer is possible. The aim of this study was therefore to determine whether α-GalCer formulated in either DMSO/Tween 80 or in liposomes, could access lymphoid tissue and stimulate immune activation following oral administration...
September 5, 2017: Journal of Pharmacy and Pharmacology
https://www.readbyqxmd.com/read/28866966/construction-of-nano-scale-liposomes-loaded-with-melatonin-via-supercritical-fluid-technology
#11
Quan Zhang, Chunfeng Ou, Shengying Ye, Xianliang Song, Shucan Luo
Melatonin-loaded liposomes (MLL) were successfully prepared using rapid expansion of supercritical solution technology. The effects of supercritical pressure on encapsulation efficiency and average particle size were then analyzed. Meanwhile, temperature, formation time and ethanol concentration in the products were studied and optimized based on the response surface methodology (RSM). An in vitro simulated digestion model was also established to evaluate the release performance of MLL. The results showed that 140 bar was the best pressure for maximizing the encapsulation efficiency value using RSM optimization, reaching up to 82...
September 4, 2017: Journal of Microencapsulation
https://www.readbyqxmd.com/read/28866151/liposomes-coated-with-thiolated-chitosan-as-drug-carriers-of-curcumin
#12
Riwang Li, Li Deng, Zhengwei Cai, Shuyun Zhang, Kun Wang, Lihua Li, Shan Ding, Changren Zhou
Liposome is one of a promising delivery system to improve water solubility, stability, and bioavailability of curcumin. But its instability is not favorable for long-circulating treatment, controlled release or conservation. To overcome the disadvantages, thiol derivatised chitosan (CSSH) were synthesized and utilized to coat liposomes. The CSSH coated curcumin liposomes (Cur-Lip-CSSH) had an encapsulation efficiency (EE) of 93.95%, a drug loading (DL) of 7.95%, an average particle size of 406.0nm, and a positive zeta-potential of 36...
November 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28865342/inclusion-of-oligonucleotide-antimicrobials-in-biocompatible-cationic-liposomes-a-structural-study
#13
Marianna Mamusa, Francesco Barbero, Costanza Montis, Laura Cutillo, Ana Gonzalez-Paredes, Debora Berti
HYPOTHESIS: Transcription factor decoys (TFD) are short oligonucleotides designed to block essential genetic pathways in bacteria and defeat resistant infections. TFD protection in biological fluids and their delivery to the site of infection require formulation in appropriate delivery systems. In this work, we build on a classical phosphatidylcholine/phosphatidylethanolamine (POPC/DOPE) scaffold to design TFD-loaded cationic liposomes by combining the DNA-complexing abilities of a bolaamphiphile, (1,1'-(dodecane-1,12-diyl)-bis-(9-amino-1,2,3,4-tetrahydroacridinium) chloride (12-bis-THA), with the biocompatible cationic lipid ethyl-phosphatidylcholine (DPePC)...
August 24, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28865290/erythrocyte-membrane-encapsulated-celecoxib-improves-the-cognitive-decline-of-alzheimer-s-disease-by-concurrently-inducing-neurogenesis-and-reducing-apoptosis-in-app-ps1-transgenic-mice
#14
Jing-Wen Guo, Pei-Pei Guan, Wei-Yan Ding, Si-Ling Wang, Xue-Shi Huang, Zhan-You Wang, Pu Wang
Alzheimer's disease (AD) is characterized by the loss of neurogenesis and excessive induction of apoptosis. The induction of neurogenesis and inhibition of apoptosis may be a promising therapeutic approach to combating the disease. Celecoxib (CB), a cyclooxygenase-2 specific inhibitor, could offer neuroprotection. Specifically, the CB-encapsulated erythrocyte membranes (CB-RBCMs) sustained the release of CB over a period of 72 h in vitro and exhibited high brain biodistribution efficiency following intranasal administration, which resulted in the clearance of aggregated β-amyloid proteins (Aβ) in neurons...
July 14, 2017: Biomaterials
https://www.readbyqxmd.com/read/28864033/characterising-the-effect-of-antimalarial-drugs-on-the-maturation-and-clearance-of-murine-blood-stage-plasmodium-parasites-in-vivo
#15
David S Khoury, Deborah Cromer, Trish Elliott, Megan S F Soon, Bryce S Thomas, Kylie R James, Shannon E Best, Rosemary A Aogo, Jessica A Engel, Kate H Gartlan, Jasmin Akter, Ismail Sebina, Ashraful Haque, Miles P Davenport
The artemisinins are the first-line therapy for severe and uncomplicated malaria, since they cause rapid declines in parasitemia after treatment. Despite this, in vivo mechanisms underlying this rapid decline remain poorly characterised. The overall decline in parasitemia is the net effect of drug inhibition of parasites and host clearance, which competes against any ongoing parasite proliferation. Separating these mechanisms in vivo was not possible through measurements of total parasitemia alone. Therefore, we employed an adoptive transfer approach in which C57BL/6J mice were transfused with Plasmodium berghei ANKA strain-infected, fluorescent red blood cells, and subsequently drug-treated...
August 31, 2017: International Journal for Parasitology
https://www.readbyqxmd.com/read/28854413/fattigation-platform-nanoparticles-using-apo-transferrin-stearic-acid-as-a-core-for-receptor-oriented-cancer-targeting
#16
Hardik H Amin, Nilesh M Meghani, Chulhun Park, Van Hong Nguyen, Thao Truong-Dinh Tran, Phuong Ha-Lien Tran, Beom-Jin Lee
A major hurdle in cancer treatment is the precise targeting of drugs to the cancer site. As many cancer cells overexpress the transferrin receptor (TfR), the transferrin (Tf)-TfR interaction is widely exploited to target cancer cells. In this study, novel amphiphilic apo-Tf stearic acid (TfS) conjugates were prepared and characterized by Fourier transform infrared (FTIR) spectroscopy, matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry, and trinitrobenzenesulfonic acid (TNBS) assay...
August 16, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28852989/biophysical-aspects-of-biomineralization
#17
REVIEW
Maytê Bolean, Ana M S Simão, Marina B Barioni, Bruno Z Favarin, Heitor G Sebinelli, Ekeveliny A Veschi, Tatiane A B Janku, Massimo Bottini, Marc F Hoylaerts, Rosangela Itri, José L Millán, Pietro Ciancaglini
During the process of endochondral bone formation, chondrocytes and osteoblasts mineralize their extracellular matrix (ECM) by promoting the synthesis of hydroxyapatite (HA) seed crystals in the sheltered interior of membrane-limited matrix vesicles (MVs). Several lipid and proteins present in the membrane of the MVs mediate the interactions of MVs with the ECM and regulate the initial mineral deposition and posterior propagation. Among the proteins of MV membranes, ion transporters control the availability of phosphate and calcium needed for initial HA deposition...
August 29, 2017: Biophysical Reviews
https://www.readbyqxmd.com/read/28852163/expression-and-purification-of-the-pain-receptor-trpv1-for-spectroscopic-analysis
#18
Phanindra Velisetty, Richard A Stein, Francisco J Sierra-Valdez, Valeria Vásquez, Julio F Cordero-Morales
The transient receptor potential vanilloid 1 (TRPV1) channel is an essential component of the cellular mechanism through which noxious stimuli evoke pain. Functional and structural characterizations of TRPV1 shed light on vanilloid activation, yet the mechanisms for temperature and proton gating remain largely unknown. Spectroscopic approaches are needed to understand the mechanisms by which TRPV1 translates diverse stimuli into channel opening. Here, we have engineered a minimal cysteine-less rat TRPV1 construct (eTRPV1) that can be stably purified and reconstituted for spectroscopic studies...
August 29, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28839228/magnetic-immuno-loop-mediated-isothermal-amplification-based-on-dna-encapsulating-liposome-for-the-ultrasensitive-detection-of-p-glycoprotein
#19
Hongmei Cao, Xueen Fang, Peng Liu, Hua Li, Weiwei Chen, Baohong Liu, Jilie Kong
Determination of proteins, especially low-abundance proteins with high sensitivity and specificity, is essential for characterizing proteomes and studying their biochemical functions. Herein, a novel Magnetic-Immuno-Loop-Mediated Isothermal Amplification (Im-LAMP) based on DNA-encapsulating liposomes (liposome-Im- LAMP), was developed for trace amounts of proteins. To the best of our knowledge, this is our first report about the magnetic Im-LAMP approach based on liposomes encapsulated template DNA as the detection reagent...
August 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28839211/a-biomimetic-microfluidic-tumor-microenvironment-platform-mimicking-the-epr-effect-for-rapid-screening-of-drug-delivery-systems
#20
Yuan Tang, Fariborz Soroush, Joel B Sheffield, Bin Wang, Balabhaskar Prabhakarpandian, Mohammad F Kiani
Real-time monitoring of tumor drug delivery in vivo is a daunting challenge due to the heterogeneity and complexity of the tumor microenvironment. In this study, we developed a biomimetic microfluidic tumor microenvironment (bMTM) comprising co-culture of tumor and endothelial cells in a 3D environment. The platform consists of a vascular compartment featuring a network of vessels cultured with endothelial cells forming a complete lumen under shear flow in communication with 3D solid tumors cultured in a tumor compartment...
August 24, 2017: Scientific Reports
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