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Liposomes characterization

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https://www.readbyqxmd.com/read/29785106/sn38-loaded-100-nm-targeted-liposomes-for-improving-poor-solubility-and-minimizing-burst-release-and-toxicity-in-vitro-and-in-vivo-study
#1
Yi-Ping Fang, Chih-Hung Chuang, Yi-Jhun Wu, Hsin-Che Lin, Yun-Chi Lu
Background: SN38 (7-ethyl-10-hydroxycamptothecin) is a camptothecin derivative acts against various tumors. However, SN38 is hydrolyzed in the physiological environment (pH 7.4), and this instability interferes with its potential therapeutic effect. Our objective was to investigate SN38-loaded liposomes to overcome the poor solubility of SN38 and its biodistribution, which further diminish its toxicity. Materials and methods: The sub-100 nm targeted liposomes was employed to deliver SN-38 and evaluate the characterization, release behaviors, cytotoxicity, in vivo pharmacokinetics and biochemical assay...
2018: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29783687/impact-of-particle-size-and-polydispersity-index-on-the-clinical-applications-of-lipidic-nanocarrier-systems
#2
REVIEW
M Danaei, M Dehghankhold, S Ataei, F Hasanzadeh Davarani, R Javanmard, A Dokhani, S Khorasani, M R Mozafari
Lipid-based drug delivery systems, or lipidic carriers, are being extensively employed to enhance the bioavailability of poorly-soluble drugs. They have the ability to incorporate both lipophilic and hydrophilic molecules and protecting them against degradation in vitro and in vivo. There is a number of physical attributes of lipid-based nanocarriers that determine their safety, stability, efficacy, as well as their in vitro and in vivo behaviour. These include average particle size/diameter and the polydispersity index (PDI), which is an indication of their quality with respect to the size distribution...
May 18, 2018: Pharmaceutics
https://www.readbyqxmd.com/read/29780479/pentacyclic-adenine-a-versatile-and-exceptionally-bright-fluorescent-dna-base-analogue
#3
Mattias Bood, Anders F Füchtbauer, Moa S Wranne, Jong Jin Ro, Sangamesh Sarangamath, Afaf H El-Sagheer, Déborah L M Rupert, Rachel S Fisher, Steven W Magennis, Anita C Jones, Fredrik Höök, Tom Brown, Byeang Hyean Kim, Anders Dahlén, L Marcus Wilhelmsson, Morten Grøtli
Emissive base analogs are powerful tools for probing nucleic acids at the molecular level. Herein we describe the development and thorough characterization of pentacyclic adenine (pA), a versatile base analog with exceptional fluorescence properties. When incorporated into DNA, pA pairs selectively with thymine without perturbing the B-form structure and is among the brightest nucleobase analogs reported so far. Together with the recently established base analog acceptor qAnitro , pA allows accurate distance and orientation determination via Förster resonance energy transfer (FRET) measurements...
April 14, 2018: Chemical Science
https://www.readbyqxmd.com/read/29771146/nanosized-ethanol-based-malleable-liposomes-of-cytarabine-to-accentuate-transdermal-delivery-formulation-optimization-in-vitro-skin-permeation-and-in-vivo-bioavailability
#4
Rakesh Raj, Pooja Mongia Raj, Alpana Ram
Cytarabine is a pyrimidine nucleoside analog used predominantly for acute myeloid leukemia (AML) and also for other indications, including acute lymphocytic leukemia, chronic myelogenous leukemia, and lymphoma by parenteral route due to its low oral bioavailability. Parenteral administration requires constant plasma level, monitoring for its fluctuation and poor patients compliances. Hence the objective of this work is to construct optimized nanosized malleable liposomes of cytarabine to accentuate transdermal delivery of drug to circumvent previously mentioned drawbacks...
May 17, 2018: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29761344/beyond-the-magic-bullet-current-progress-of-therapeutic-vaccination-in-multiple-sclerosis
#5
Barbara Willekens, Nathalie Cools
Multiple sclerosis (MS) is a chronic immune-mediated disease of the central nervous system (CNS) characterized by neuroinflammation, neurodegeneration and impaired repair mechanisms that lead to neurological disability. The crux of MS is the patient's own immune cells attacking self-antigens in the CNS, namely the myelin sheath that protects nerve cells of the brain and spinal cord. Restoring antigen-specific tolerance via therapeutic vaccination is an innovative and exciting approach in MS therapy. Indeed, leveraging the body's attempt to prevent autoimmunity, i...
May 14, 2018: CNS Drugs
https://www.readbyqxmd.com/read/29760126/experimental-amphotericin-b-deoxycholate-formulations-for-pulmonary-aspergillosis-efficacy-biodistribution-and-nephrotoxicity
#6
Alicia López-Sánchez, Alba Pérez-Cantero, Carlos Torrado-Salmerón, Adela Martin-Vicente, Víctor García-Herrero, María Ángeles González-Nicolás, Alberto Lázaro, Alberto Tejedor, Santiago Torrado-Santiago, Juan José García-Rodríguez, Javier Capilla, Susana Torrado
An experimental micellar formulation of amphotericin B (AMB) with sodium deoxycholate (DCH), AMB:DCH 1:1.5, was obtained and characterized to determine its aggregation state and particle size. Biodistribution, nephrotoxicity and efficacy against pulmonary aspergillosis in a murine model were studied and compared to the liposomal commercial amphotericin B after intravenous administration. The administration of 5 mg/kg AMB:DCH 1:1.5 presented 2.8-fold higher lung concentrations (18.125 ± 3.985 μg/g, after 6 daily doses) and lower kidney exposure (0...
May 14, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29755535/optimization-and-characterization-of-aspirin-encapsulated-nano-liposomes
#7
Samira Khodayar, Hassan Bardania, Seyed Abbas Shojaosadati, Fatemeh Bagheri
Resistance to aspirin and its cytotoxicity significantly limits its therapeutic applications. Nano-liposomal encapsulation of aspirin can reduce its cytotoxicity. In this study, aspirin encapsulating nano-liposomes (AS-NL) was prepared and its performance in drug delivery and also cytotoxicity was evaluated. The effects of two independent variables including number of freeze/thawing cycles and concentration of aspirin on encapsulation efficiency was investigated using response surface methodology (RSM). A drug profile release was obtained by AS-NL...
2018: Iranian Journal of Pharmaceutical Research: IJPR
https://www.readbyqxmd.com/read/29752967/hiv-1-matrix-protein-interactions-with-trna-implications-for-membrane-targeting
#8
Christy R Gaines, Emre Tkacik, Amalia Rivera-Oven, Phoebe Somani, Alecia Achimovich, Tawakalitou Alabi, Angela Zhu, Noel Getachew, Ae Lim Yang, Matthew McDonough, Tarik Hawkins, Zoe Spadaro, Michael F Summers
The N-terminally myristoylated matrix domain (MA) of the HIV-1 Gag polyprotein promotes virus assembly by targeting Gag to the inner leaflet of the plasma membrane (PM). Recent studies indicate that, prior to membrane binding, MA associates with cytoplasmic tRNAs (including tRNALys3 ), and in vitro studies of tRNA-dependent MA interactions with model membranes have led to proposals that competitive tRNA interactions contribute to membrane discrimination. We have characterized interactions between native, mutant, and unmyristylated (myr-) MA proteins and recombinant tRNALys3 by NMR spectroscopy and isothermal titration calorimetry (ITC)...
May 9, 2018: Journal of Molecular Biology
https://www.readbyqxmd.com/read/29744848/model-phospholipid-liposomes-to-study-the-%C3%AE-amyloid-peptide-induced-membrane-disruption
#9
Wei Qiang, Katelynne E Doherty
Model phospholipid liposomes have been utilized widely to study the molecular interactions between peptides and membrane bilayers. In the mechanistic study of Alzheimer's disease (AD), disruption of neuronal cell membranes has been considered as a major contribution for the β-amyloid (Aβ) peptides' neurotoxicity. However, clear interpretation of the Aβ-induced cellular membrane at high-resolution level is challenging because of the co-existence of multiple pathways. Here we present the generation of simplified model liposome systems that will facilitate the in-depth mechanistic studies...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29744672/efficacy-and-safety-of-neoadjuvant-treatment-with-bevacizumab-liposomal-doxorubicin-cyclophosphamide-and-paclitaxel-combination-in-locally-regionally-advanced-her2-negative-grade-iii-at-premenopausal-status-breast-cancer-a-phase-ii-study
#10
Ekaterini C Tampaki, Athanasios Tampakis, Constantinos E Alifieris, Dimitrios Krikelis, Anastasia Pazaiti, Michalis Kontos, Dimitrios T Trafalis
BACKGROUND: In the era of personalized therapy, targeted treatment in specific patient populations is mandated. OBJECTIVE: We evaluated the efficacy and safety of neoadjuvant treatment on locally advanced breast cancer (LABC) with a monoclonal agent against vascular endothelial growth factor (VEGF), bevacizumab plus chemotherapy combination of liposomal doxorubicin, cyclophosphamide and paclitaxel (PLAC-B). METHODS: Patients enrolled were at premenopausal status and characterized by human epidermal growth factor receptor 2 (HER2)-negative, hormone-receptor positive (estrogen receptor/progesterone receptor-positive [ER/PR+]) or triple-negative (TNBC), LABC (T > 3 cm), with high-grade ductal carcinoma...
May 9, 2018: Clinical Drug Investigation
https://www.readbyqxmd.com/read/29741759/anionic-nanoliposomes-reduced-atherosclerosis-progression-in-low-density-lipoprotein-receptor-ldlr-deficient-mice-fed-a-high-fat-diet
#11
Smriti M Krishna, Joseph V Moxon, Roby J Jose, Jiaze Li, Amirhossein Sahebkar, Mahmoud R Jaafari, Mahdi Hatamipour, Dawie Liu, Jonathan Golledge
Atherosclerosis is a systemic disease characterized by the deposition of cholesterol and inflammatory cells within the arterial wall. Removal of cholesterol from the vessel wall may have an impact on the size and composition of atherosclerotic lesions. Anionic phospholipids or liposome vesicles composed of a lipid bilayer such as nanoliposomes have been suggested as treatments for dyslipidemia. In this study, we investigated the effect of anionic nanoliposomes on atherosclerosis in a mouse model. Low-density lipoprotein receptor knockout mice (Ldlr-/- ) were fed with an atherosclerosis promoting high fat and cholesterol (HFC) diet for 12 weeks...
May 9, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29730004/in-vitro-and-in-vivo-characterization-of-quercetin-loaded-multiphase-hydrogel-for-wound-healing-application
#12
Rajendra Jangde, Shikha Srivastava, Manju R Singh, Deependra Singh
The present work aim to prepare and evaluate multiphase hydrogel system incorporated with quercetin loaded liposomes (QLH), for wound healing. The quercetin loaded liposomal hydrogel were prepared by taking 15% carbopol and varying gelatin ratio. The clear and transparent hydrogel was obtained by taking ratio of gelatin to carbapol (6/4) compared to other ratios. The best prepared hydrogel were characterized for surface morphology, water vapor transmission rate (WVTR), swelling ratio, hemocompatibility, stability, in-vitro release and in-vivo studies...
May 3, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29723469/automated-removal-of-phospholipids-from-membrane-proteins-for-h-d-exchange-mass-spectrometry-workflows
#13
Kyle W Anderson, Elyssia S Gallagher, Jeffrey W Hudgens
Membrane proteins are currently the most common targets for pharmaceuticals. However, characterization of their structural dynamics by hydrogen/deuterium exchange mass spectrometry (HDX-MS) is sparse due to insufficient automated methods to handle full-length membrane proteins in lipid bilayers. Additionally, membrane lipids used to mimic the membrane environment and to solubilize membrane proteins can impair chromatography performance and cause ion suppression in the mass spectrometer. The workflow discussed herein advances HDX-MS capabilities and other MS applications for membrane proteins by providing a fully automated method for HDX-MS analysis based on a phospholipid removal scheme compatible with robotic handling...
May 9, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29723251/spatiotemporal-assessment-of-spontaneous-metastasis-formation-using-multimodal-in-vivo-imaging-in-her2-and-triple-negative-metastatic-breast-cancer-xenograft-models-in-mice
#14
Inga B Fricke, Raquel De Souza, Lais Costa Ayub, Giulio Francia, Robert Kerbel, David A Jaffray, Jinzi Zheng
BACKGROUND: Preclinical breast cancer models recapitulating the clinical course of metastatic disease are crucial for drug development. Highly metastatic cell lines forming spontaneous metastasis following orthotopic implantation were previously developed and characterized regarding their biological and histological characteristics. This study aimed to non-invasively and longitudinally characterize the spatiotemporal pattern of metastasis formation and progression in the MDA-MB-231-derived triple negative LM2-4 and HER2+ LM2-4H2N cell lines, using bioluminescence imaging (BLI), contrast enhanced computed tomography (CT), fluorescence imaging, and 2-deoxy-2-[fluorine-18]fluoro-D-glucose positron emission tomography ([18F]FDG-PET)...
2018: PloS One
https://www.readbyqxmd.com/read/29722421/carbon-nanotubes-cnts-based-advanced-dermal-therapeutics-current-trends-and-future-potential
#15
REVIEW
Kaushik Kuche, Rahul Maheshwari, Vishakha Tambe, Kit-Kay Mak, Hardi Jogi, Nidhi Raval, Mallikarjuna Rao Pichika, Rakesh Kumar Tekade
The search for effective and non-invasive delivery modules to transport therapeutic molecules across skin has led to the discovery of a number of nanocarriers (viz.: liposomes, ethosomes, dendrimers, etc.) in the last few decades. However, available literature suggests that these delivery modules face several issues including poor stability, low encapsulation efficiency, and scale-up hurdles. Recently, carbon nanotubes (CNTs) emerged as a versatile tool to deliver therapeutics across skin. Superior stability, high loading capacity, well-developed synthesis protocol as well as ease of scale-up are some of the reason for growing interest in CNTs...
May 3, 2018: Nanoscale
https://www.readbyqxmd.com/read/29721081/companion-diagnostic-64-cu-liposome-positron-emission-tomography-enables-characterization-of-drug-delivery-to-tumors-and-predicts-response-to-cancer-nanomedicines
#16
Helen Lee, Daniel Gaddy, Manuela Ventura, Nicholas Bernards, Raquel de Souza, Dmitri Kirpotin, Thomas Wickham, Jonathan Fitzgerald, Jinzi Zheng, Bart S Hendriks
Deposition of liposomal drugs into solid tumors is a potentially rate-limiting step for drug delivery and has substantial variability that may influence probability of response. Tumor deposition is a shared mechanism for liposomal therapeutics such that a single companion diagnostic agent may have utility in predicting response to multiple nanomedicines. Methods: We describe the development, characterization and preclinical proof-of-concept of the positron emission tomography (PET) agent, MM-DX-929, a drug-free untargeted 100 nm PEGylated liposome stably entrapping a chelated complex of 4-DEAP-ATSC and 64 Cu (copper-64)...
2018: Theranostics
https://www.readbyqxmd.com/read/29709613/impact-of-membrane-curvature-on-amyloid-aggregation
#17
REVIEW
Mayu S Terakawa, Yuxi Lin, Misaki Kinoshita, Shingo Kanemura, Dai Itoh, Toshihiko Sugiki, Masaki Okumura, Ayyalusamy Ramamoorthy, Young-Ho Lee
The misfolding, amyloid aggregation, and fibril formation of intrinsically disordered proteins/peptides (or amyloid proteins) have been shown to cause a number of disorders. The underlying mechanisms of amyloid fibrillation and structural properties of amyloidogenic precursors, intermediates, and amyloid fibrils have been elucidated in detail; however, in-depth examinations on physiologically relevant contributing factors that induce amyloidogenesis and lead to cell death remain challenging. A large number of studies have attempted to characterize the roles of biomembranes on protein aggregation and membrane-mediated cell death by designing various membrane components, such as gangliosides, cholesterol, and other lipid compositions, and by using various membrane mimetics, including liposomes, bicelles, and different types of lipid-nanodiscs...
April 27, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29702340/in-vitro-photodynamic-activity-of-lipid-vesicles-with-zinc-phthalocyanine-derivative-against-enterococcus-faecalis
#18
Lukasz Sobotta, Jolanta Dlugaszewska, Piotr Kasprzycki, Sebastian Lijewski, Anna Teubert, Jadwiga Mielcarek, Maria Gdaniec, Tomasz Goslinski, Piotr Fita, Ewa Tykarska
Zinc(II) phthalocyanine bearing eight non-peripheral 2-propoxy substituents was subjected to physicochemical study and, after incorporation in lipid vesicles, assessed as a potential photosensitizer for antibacterial photodynamic therapy. The phthalocyanine derivative obtained in the macrocyclization reaction was characterized by MS and NMR techniques. Moreover, its chemical purity was confirmed by HPLC analysis. X-ray structural analysis revealed that overcrowding of the phthalocyanine derivative leads to a strong out-of-plane distortion of the π-system of the macrocycle core...
April 17, 2018: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29695068/cationic-liposomes-carrying-sirna-impact-of-lipid-composition-on-physicochemical-properties-cytotoxicity-and-endosomal-escape
#19
Anna Lechanteur, Vincent Sanna, Amandine Duchemin, Brigitte Evrard, Denis Mottet, Géraldine Piel
In recent year, cationic liposomes have gained a lot of attention for siRNA delivery. Despite this, intracellular barriers as endosomal escape and cytosolic delivery of siRNA still represent a challeng, as well as the cytotoxicity due to cationic lipids. To address these issues, we developed four liposomal formulations, composed of two different cationic lipids (DOTAP and DC-Cholesterol) and different ratio of co-lipids (cholesterol and DOPE). The objective is to dissect these impacts on siRNA efficacy and cytotoxicity...
April 24, 2018: Nanomaterials
https://www.readbyqxmd.com/read/29694498/production-characterization-and-toxicology-assay-of-creatine-pegylated-nanoliposome-with-polysorbate-80-for-brain-delivery
#20
Diego B Borin, Nathana J Mezzomo, Rodrigo A Vaucher, Guilherme DO Carmo, Luiz C Rodrigues Junior, Fernando B Sulczewski, Claiton I Schwertz, Ricardo E Mendes, Adriani P Damiani, Vanessa M DE Andrade, Virgínia C Rech, Carina R Boeck
Creatine acts intracellularly as energy buffer and storage, demonstrating protective effects in animal models of neurodegenerative diseases. However, its permeability throught blood-brain barrier (BBB) is reduced. The aim of the present study was developing a carrier to facilitate the delivery of creatine to the central nervous system. Creatine nanoliposomes were produced, characterized and assayed in models of toxicity in vitro and in vivo. Particles showed negative zeta potential (-12,5 mV), polydispersity index 0...
April 23, 2018: Anais da Academia Brasileira de Ciências
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