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Liposomes characterization

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https://www.readbyqxmd.com/read/28221378/characterization-of-drug-encapsulation-and-retention-in-archaea-inspired-tetraether-liposomes
#1
Geoffray Leriche, Jessica L Cifelli, Kevin C Sibucao, Joseph P Patterson, Takaoki Koyanagi, Nathan C Gianneschi, Jerry Yang
The passive leakage of small molecules across membranes is a major limitation of liposomal drug formulations. Here, we evaluate the leakage of 3 clinically used chemotherapeutic agents (cytarabine, methotrexate and vincristine) encapsulated in liposomes comprised of a synthetic, archaea-inspired, membrane-spanning tetraether lipid. Liposomes comprised of the pure tetraether lipid exhibited superior retention of both a neutrally and positively charged drug (up to an ∼9-fold decrease in the rate of drug leakage) compared to liposomes formed from a commercial diacyl lipid, while exhibiting a similar retention of a negatively charged drug that did not appreciably leak from either type of liposome...
February 21, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28216150/novel-biodegradable-poly-gamma-glutamic-acid-amphotericin-b-complexes-show-promise-as-improved-amphotericin-b-formulations
#2
T Dinh, Q Zia, S Zubair, P Stapleton, R Singh, M Owais, S Somavarapu
Commercially available amphotericin B (AmB) formulations are limited either by cytotoxicities, lower efficacies, shelf-life related issues or high production costs. AmB complexes based on poly(gamma-glutamic acid) (PGGA) have been prepared and evaluated for their efficacies against AmB-deoxycholate (Fungizone®) and liposomal AmB (AmBisome®). Physical characterizations showed that AmB/PGGA complexes are nanoscopic (20-40nm) with a negative zetapotential (-51.0mV), water-soluble, stable in solution (up to 4weeks, at 4 °C and 25 °C), and have a theoretical drug loading (up to 76...
February 16, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/28208292/nanoviscosity-measurements-revealing-domain-formation-in-biomimetic-membranes
#3
Imad Younus Hasan, Adam Mechler
Partitioning of lipid molecules in biomimetic membranes is a model system for the study of naturally occurring domains, such as rafts, in biological membranes. The existence of nanometer scale membrane domains in binary lipid mixtures has been shown with microscopy methods; however, the nature of these domains has not been established unequivocally. A common notion is to ascribe domain separation to thermodynamic phase equilibria. However, characterizing thermodynamic phases of single bilayer membranes has not been possible due to their extreme dimensions: the size of the domains falls to the order of tens to hundreds of nanometers whereas the membrane thickness is only a few nanometers...
February 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28207486/particle-based-delivery-of-the-hiv-envelope-protein
#4
Benedikt Asbach, Ralf Wagner
PURPOSE OF REVIEW: A major focus in HIV vaccine research is the development of suitable antigens that elicit broadly neutralizing antibody responses targeting HIV's envelope protein (Env). Delivery of Env in a repetitive manner on particle-based carriers allows higher avidity interactions and is therefore expected to efficiently engage B cells, thus leading to affinity maturation that results in superior antibody responses characterized by improved breadth, potency, and durability. This review summarizes current work that is evaluating diverse types of such particulate carriers for Env delivery...
February 15, 2017: Current Opinion in HIV and AIDS
https://www.readbyqxmd.com/read/28203076/light-triggered-liposomal-cargo-delivery-platform-incorporating-photosensitizers-and-gold-nanoparticles-for-enhanced-singlet-oxygen-generation-and-increased-cytotoxicity
#5
Zofia Kautzka, Sandhya Clement, Ewa M Goldys, Wei Deng
We developed light-triggered liposomes incorporating 3-5 nm hydrophobic gold nanoparticles and Rose Bengal (RB), a well-known photosensitizer used for photodynamic therapy. Singlet oxygen generated by these liposomes with 532 nm light illumination was characterized for varying the molar ratio of lipids and gold nanoparticles while keeping the amount of RB constant. Gold nanoparticles were found to enhance the singlet oxygen generation rate, with a maximum enhancement factor of 1.75 obtained for the molar ratio of hydrogenated soy l-α-phosphatidylcholine:1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(hexanoylamine):gold of 57:5:17 compared with liposomes loaded with RB alone...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28196324/delivery-of-liposomal-quantum-dots-via-monocytes-for-imaging-of-inflamed-tissue
#6
Gil Aizik, Nir Waiskopf, Majd Agbaria, Yael Levi-Kalisman, Uri Banin, Gershon Golomb
Quantum dots (QDs), semiconductor nanocrystals, are fluorescent nanoparticles of growing interest as an imaging tool of a diseased tissue. However, a major concern is their biocompatibility, cytotoxicity, and fluorescence instability in biological milieu, impeding their use in biomedical applications, in general, and for inflammation imaging, in particular. In addition, for an efficient fluorescent signal at the desired tissue, and avoiding systemic biodistribution and possible toxicity, targeting is desired...
February 20, 2017: ACS Nano
https://www.readbyqxmd.com/read/28192250/bifunctional-viscous-nanovesicles-co-loaded-with-resveratrol-and-gallic-acid-for-skin-protection-against-microbial-and-oxidative-injuries
#7
Justina Vitonyte, Maria Letizia Manca, Carla Caddeo, Donatella Valenti, Josè Esteban Peris, Iris Usach, Amparo Nacher, Maria Matos, Gemma Gutiérrez, Germano Orrù, Xavier Fernàndez-Busquets, Anna Maria Fadda, Maria Manconi
Resveratrol and gallic acid were co-loaded in phospholipid vesicles aiming at protecting the skin from external injuries, such as oxidative stress and microbial infections. Liposomes were prepared using biocompatible phospholipids dispersed in water. To improve vesicle stability and applicability, the phospholipids and the phenols were dispersed in water/propylene glycol or water/glycerol, thus obtaining PEVs and glycerosomes, respectively. The vesicles were characterized by size, morphology, physical stability, and their therapeutic efficacy was investigated in vitro...
February 10, 2017: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/28191842/synthesis-and-functional-characterization-of-novel-sialyl-lewisx-mimic-decorated-liposomes-for-e-selectin-mediated-targeting-to-inflamed-endothelial-cells
#8
Chanikarn Chantarasrivong, Akiharu Ueki, Ryutaro Ohyama, Johan Unga, Shinya Nakamura, Isao Nakanishi, Yuriko Higuchi, Shigeru Kawakami, Hiromune Ando, Akihiro Imamura, Hideharu Ishida, Fumiyoshi Yamashita, Makoto Kiso, Mitsuru Hashida
Sialyl LewisX (sLeX) is a natural ligand of E-selectin that is overexpressed by inflamed and tumor endothelium. Although sLeX is a potential ligand for drug targeting, synthesis of the tetrasaccharide is complicated with many reaction steps. In this study, structurally simplified novel sLeX analogs were designed and linked with 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-polyethyleneglycol-2000 (DSPE-PEG) for E-selectin-mediated liposomal delivery. The sLeX structural simplification strategies include (1) replacement of the Gal-GlcNAc disaccharide unit with lactose to reduce many initial steps and (2) substitution of neuraminic acid with a negatively charged group, i...
February 13, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28190740/protective-efficacy-of-a-lipid-antigen-vaccine-in-a-guinea-pig-model-of-tuberculosis
#9
Gérald Larrouy-Maumus, Emilie Layre, Simon Clark, Jacques Prandi, Emma Rayner, Marco Lepore, Gennaro de Libero, Ann Williams, Germain Puzo, Martine Gilleron
The bacillus Calmette Guérin (BCG) vaccine, the only licensed vaccine against TB, displays partial and variable efficacy, thus making the exploitation of novel vaccination strategies a major priority. Most of the current vaccines in pre-clinical or clinical development are based on the induction of T cells recognizing protein antigens. However, a large number of T cells specific for mycobacterial lipids are induced during infection, suggesting that lipid-based vaccines might represent an important component of novel sub-unit vaccines...
February 9, 2017: Vaccine
https://www.readbyqxmd.com/read/28186461/aceclofenac-cocrystal-nanoliposomes-for-rheumatoid-arthritis-with-better-dermatokinetic-attributes-a-preclinical-study
#10
Gajanand Sharma, Mandeep Kaur Saini, Kanika Thakur, Namarta Kapil, Neeraj Kumar Garg, Kaisar Raza, Vijay G Goni, Anil Pareek, Om Prakash Katare
AIM: The aim of present research was to complex aceclofenac with lysine (LYS) and the developed aceclofenac-LYS cocrystal was encapsulated in lipid bilayers of liposomes by employing dual carrier approach for the treatment of pain-related disorders in rheumatoid arthritis (RA). MATERIALS & METHODS: The developed carriers were characterized for particle size, drug release, ex vivo and in vivo studies, dermatokinetic modeling, complete freund's adjuvant (CFA)-induced RA rat model, radiant heat tail-flick method, formalin-induced paw-licking model, paw edema model and xylene-induced ear edema model in mice...
February 10, 2017: Nanomedicine
https://www.readbyqxmd.com/read/28183626/stavudine-loaded-gelatin-liposomes-for-hiv-therapy-preparation-characterization-and-in-vitro-cytotoxic-evaluation
#11
Debasis Nayak, Ankita Boxi, Sarbani Ashe, Neethi Chandra Thathapudi, Bismita Nayak
Despite continuous research and availability of 25 different active compounds for treating chronic HIV-1 infection, there is no absolute cure for this deadly disease. Primarily, the residual viremia remains hidden in latently infected reservoir sites and persistently release the viral RNA into the blood stream. The study proposes the dual utilization of the prepared stavudine-containing nanoformulations to control the residual viremia as well as target the reservoir sites. Gelatin nanoformulations containing very low dosage of stavudine were prepared through classical desolvation process and were later loaded in soya lecithin-liposomes...
April 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28183623/the-role-of-nanoparticles-in-the-albumin-cytarabine-and-albumin-methotrexate-interactions
#12
Danuta Pentak, Małgorzata Maciążek-Jurczyk, Zygmunt H Zawada
Understanding the interactions which occur between nanomaterials and biomolecules is one of the most important issues in nanotechnology. Determining the properties of nanoparticles obtained through the use of novel methods and defining the scope of their application as drug carriers has important practical significance. Nanoparticles containing methotrexate and cytarabine obtained by a modified reverse-phase evaporation method (mREV) were characterized through the use of the UV/Vis and NMR methods. Obtained results confirmed high degree of analysed drugs encapsulation...
April 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28174679/engineering-remotely-triggered-liposomes-to-target-triple-negative-breast-cancer
#13
Alexandra Sneider, Rahul Jadia, Brandon Piel, Derek VanDyke, Christopher Tsiros, Prakash Rai
Triple Negative Breast Cancer (TNBC) continues to present a challenge in the clinic, as there is still no approved targeted therapy. TNBC is the worst sub-type of breast cancer in terms of prognosis and exhibits a deficiency in estrogen, progesterone, and human epidermal growth factor 2 (HER2) receptors. One possible option for the treatment of TNBC is chemotherapy. The issue with many chemotherapy drugs is that their effectiveness is diminished due to poor water solubility, and the method of administration directly or with a co-solvent intravenously can lead to an increase in toxicity...
2017: Oncomedicine
https://www.readbyqxmd.com/read/28165817/pcm-and-tat-co-modified-liposome-with-improved-myocardium-delivery-in-vitro-and-in-vivo-evaluations
#14
Xin Wang, Hua Huang, Liangke Zhang, Yan Bai, Huali Chen
In this study, PCM and TAT co-modified liposome was developed as a novel drug carrier for myocardium delivery with evaluation of its in vitro and in vivo properties. Liposomes containing fluorescent probe coumarin-6 were prepared by thin-film hydration. The PCM ligands specifically bind to the PCM receptors in the extracellular connective tissue of primary myocardium cells (MCs), while the TAT ligands functioned as a classical cell penetrating peptide to make liposomes internalized by MCs. The unmodified liposome (L), PCM-modified liposome (PL), TAT-modified liposome (TL) and PCM and TAT co-modified liposome (PTL) were prepared and characterized...
November 2017: Drug Delivery
https://www.readbyqxmd.com/read/28165805/development-and-characterization-of-polymer-coated-liposomes-for-vaginal-delivery-of-sildenafil-citrate
#15
Hanan Refai, Doaa Hassan, Rehab Abdelmonem
Vaginal administration of sildenafil citrate has shown recently to develop efficiently the uterine lining with subsequent successful embryo implantation following in vitro fertilization. The aim of the present study was to develop sildenafil-loaded liposomes coated with bioadhesive polymers for enhanced vaginal retention and improved drug permeation. Three liposomal formulae were prepared by thin-film method using different phospholipid:cholesterol ratios. The optimal liposomal formulation was coated with bioadhesive polymers (chitosan and HPMC)...
November 2017: Drug Delivery
https://www.readbyqxmd.com/read/28161665/lipid-nanoparticles-assessment-by-flow-cytometry
#16
Anna Bryła, Wojciech Juzwa, Marek Weiss, Grażyna Lewandowicz
BACKGROUND: Liposomes are promising carriers for drugs and bioactive compounds. Size and structure are their crucial parameters. Thus, it is essential to assess individual vesicles as prepared. Currently available techniques fail to measure liposome's size and structure simultaneously, with a high throughput. To solve this problem, we have developed a novel, flow cytometric method quantifying liposomes. METHODS: Firstly, the following fluorescent staining combinations were tested: DiD/TO, Rh123/DiD, Syto9/DiD...
February 1, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28160164/liposomal-drug-product-development-and-quality-current-us-experience-and-perspective
#17
Mamta Kapoor, Sau L Lee, Katherine M Tyner
Research in the area of liposomes has grown substantially in the past few decades. Liposomes are lipid bilayer structures that can incorporate drug substances to modify the drug's pharmacokinetic profile thereby improving drug delivery. The agency has received over 400 liposomal drug product submissions (excluding combination therapies), and there are currently eight approved liposomal drug products on the US market. In order to identify the pain points in development and manufacturing of liposomal drug products, a retrospective analysis was performed from a quality perspective on submissions for new and generic liposomal drug products...
February 3, 2017: AAPS Journal
https://www.readbyqxmd.com/read/28155540/optimizing-novel-penetration-enhancing-hybridized-vesicles-for-augmenting-the-in-vivo-effect-of-an-anti-glaucoma-drug
#18
Sarah S Naguib, Rania M Hathout, Samar Mansour
Usually the topical delivery of ocular drugs poses a great challenge. Accordingly, the work in this study comprised the use of different hybrids of generally regarded as safe (GRAS) oils and surfactants in order to develop and optimize novel acetazolamide (AZD) entrapped-vesicular systems aiming at improving its ocular delivery and reaching better therapeutic outcomes in the treatment of glaucoma. The phospholipid/cholesterol bilayer of the vesicles was enriched with hybrids of Tween 80, Labrasol, Transcutol and Labrafac lipophile WL in different masses and proportions according to a mixture design viz...
November 2017: Drug Delivery
https://www.readbyqxmd.com/read/28153496/the-daunorubicin-interplay-with-mimetic-model-membranes-of-cancer-cells-a-biophysical-interpretation
#19
Ana Catarina Alves, Daniela Ribeiro, Miguel Horta, José L F C Lima, Cláudia Nunes, Salette Reis
The present work aimed to study the interactions between the anticancer drug daunorubicin and lipid membrane mimetic models of cancer cells composed by their most representative classes of phospholipids, with different degrees of complexity. Regarding these anticancer drug-membrane interactions, several biophysical parameters were assessed using liposomes (LUVs) composed of different molar ratios of DMPC, DOPC, DPPS, DOPE and Chol. In this context, daunorubicin's membrane concentration was determined by calculating its partition coefficient (Kp) between liposomes and water using derivative UV/vis spectrophotometry at 37°C and pH6...
January 30, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28147550/characterization-of-the-interface-of-binary-mixed-dopc-dops-liposomes-in-water-the-impact-of-charge-condensation
#20
Cornelis Lütgebaucks, Carlos Macias-Romero, Sylvie Roke
Solutions of liposomes composed of binary mixtures of anionic dioleoylphosphatidylserine (DOPS) and zwitterionic dioleoylphosphatidylcholine (DOPC) are investigated with label-free angle-resolved (AR) second harmonic scattering (SHS) and electrophoretic mobility measurements. The membrane surface potential is extracted from the AR-SHS response. The surface potential changes from -10 to -145 mV with varying DOPS content ( from 0% to 100%) and levels off already at ∼ 10 % DOPS content. The ζ-potential shows the same trend but with a drastically lower saturation value (-44 mV)...
January 28, 2017: Journal of Chemical Physics
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