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Liposomes characterization

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https://www.readbyqxmd.com/read/28530812/probing-the-binding-modes-of-a-multi-domain-protein-to-lipid-based-nanoparticles-by-relaxation-based-nmr
#1
Alberto Ceccon, Vitali Tugarinov, Andrew J Boughton, David Fushman, G Marius Clore
The interactions of two model multi-domain proteins - covalently linked di-ubiquitins, Ub2 - with lipid-based nanoparticles have been quantitatively probed by the measurements of NMR lifetime line broadening, ΔR2. By combined analysis of ΔR2 profiles arising from interactions with liposomes of varying sizes, an approach recently developed for the characterization of interactions of mono-ubiquitin with liposomes, we determine how the parameters of exchange (liposome binding) and dynamics of each individual domain of Ub2 on the surface of liposomes change when the domains are covalently attached to one another by a flexible linker...
May 22, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/28528112/microencapsulation-and-characterization-of-liposomal-vesicles-using-a-supercritical-fluid-process-coupled-with-vacuum-driven-cargo-loading
#2
Wen-Chyan Tsai, Syed S H Rizvi
A new technique of liposomal microencapsulation, consisting of supercritical fluid extraction followed by rapid expansion of the supercritical solution and vacuum-driven cargo loading, was successfully developed. It is a continuous flow-through process without usage of any toxic organic solvent. For use as a coating material, the solubility of soy phospholipids in supercritical carbon dioxide was first determined using a dynamic equilibrium system and the data was correlated with the Chrastil model with good agreement...
June 2017: Food Research International
https://www.readbyqxmd.com/read/28524721/thermoresponsive-pegylated-bubble-liposome-nanovectors-for-efficient-sirna-delivery-via-endosomal-escape
#3
Kholod Alamoudi, Patricia Martins, Jonas G Croissant, Sachin Patil, Haneen Omar, Niveen M Khashab
AIM: Improving the delivery of siRNA into cancer cells via bubble liposomes. Designing a thermoresponsive pegylated liposome through the introduction of ammonium bicarbonate salt into liposomes so as to control their endosomal escape for gene therapy. METHODS: A sub-200 nm nanovector was fully characterized and examined for cellular uptake, cytotoxicity, endosomal escape and gene silencing. RESULTS: The siRNA-liposomes were internalized into cancer cells within 5 min and then released siRNAs in the cytosol prior to lysosomal degradation upon external temperature elevation...
May 19, 2017: Nanomedicine
https://www.readbyqxmd.com/read/28513597/thermophoretic-migration-of-vesicles-depends-on-mean-temperature-and-head-group-chemistry
#4
Emma L Talbot, Jurij Kotar, Lucia Parolini, Lorenzo Di Michele, Pietro Cicuta
A number of colloidal systems, including polymers, proteins, micelles and hard spheres, have been studied in thermal gradients to observe and characterize their driven motion. Here we show experimentally the thermophoretic behaviour of unilamellar lipid vesicles, finding that mobility depends on the mean local temperature of the suspension and on the structure of the exposed polar lipid head groups. By tuning the temperature, vesicles can be directed towards hot or cold, forming a highly concentrated region...
May 17, 2017: Nature Communications
https://www.readbyqxmd.com/read/28504409/multifunctional-liposomes-for-image-guided-intratumoral-chemo-phototherapy
#5
Dyego Miranda, Kevin Carter, Dandan Luo, Shuai Shao, Jumin Geng, Changning Li, Upendra Chitgupi, Steven G Turowski, Nasi Li, G Ekin Atilla-Gokcumen, Joseph A Spernyak, Jonathan F Lovell
Intratumoral (IT) drug injections reduce systemic toxicity, but delivered volumes and distribution can be inconsistent. To improve IT delivery paradigms, porphyrin-phospholipid (PoP) liposomes are passively loaded with three hydrophilic cargos: sulforhodamine B, a fluorophore; gadolinium-gadopentetic acid, a magnetic resonance (MR) agent; and oxaliplatin, a colorectal cancer chemotherapeutic. Liposome composition is optimized so that cargo is retained in serum and storage, but is released in less than 1 min with exposure to near infrared light...
May 15, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28503066/essential-oil-mediated-glycerosomes-increase-transdermal-paeoniflorin-delivery-optimization-characterization-and-evaluation-in-vitro-and-in-vivo
#6
Kai Zhang, Yongtai Zhang, Zhe Li, Nana Li, Nianping Feng
In this study, a novel glycerosome carrier containing essential oils was prepared for topical administration of paeoniflorin (PF) to enhance its transdermal drug delivery and improve drug absorption in the synovium. The formulation of glycerosomes was optimized by a uniform design, and the final vehicle was composed of 5% (w/v) phospholipid, 0.6% (w/v) cholesterol, and 10% (v/v) glycerol, with 2% (v/v) Speranskia tuberculata essential oil (STO) as the transdermal enhancer. The in vitro transdermal flux of PF loaded in the STO-glycerosomes was 1...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28502122/humanized-nog-mice-as-a-model-for-tuberculosis-vaccine-induced-immunity-a-comparative-analysis-with-the-mouse-and-guinea-pig-models-of-tuberculosis
#7
Ajay Grover, Amber Troy, Jenny Rowe, JoLynn M Troudt, Elizabeth Creissen, Jennifer McLean, Prabal Banerjee, Gerold Feuer, Angelo A Izzo
The humanized mouse model has been developed as a model to identify and characterize human immune responses to human pathogens and has been used to better identify vaccine candidates. In the current studies, the humanized mouse was used to determine the ability of a vaccine to affect the immune response to infection with Mycobacterium tuberculosis. Both human CD4(+) and CD8(+) T cells responded to infection in humanized mice as a result of infection. In humanized mice vaccinated with either BCG or with CpG-C, a liposome-based formulation containing the M...
May 14, 2017: Immunology
https://www.readbyqxmd.com/read/28499202/novel-dual-ves-phospholipid-self-assembled-liposomes-with-an-extremely-high-drug-loading-efficiency
#8
Yongpeng Hou, Chen Yao, Longbing Ling, Yawei Du, Ruiyu He, Muhammad Ismail, Ying Zhang, Zhenglin Fu, Xinsong Li
Vitamin E succinate (VES), a unique selective anti-cancer drug, has attracted much attention for its ability to induce apoptosis in various cancer cells. Importantly, it has been reported that VES is largely non-toxic to normal cells. However, poor aqueous solubility and bioavailability extensively restricted its clinical utility. In this report, dual VES phospholipid conjugate (di-VES-GPC) prodrug based liposomes were prepared in order to develop an efficient delivery system for VES. Di-VES-GPC was first synthesized by conjugating VES with l-α-glycerophosphorylcholine (GPC) using N,N'-dicyclohexylcarbodiimide (DCC) as a coupling agent...
May 2, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28497975/adjuvants-based-on-synthetic-mycobacterial-cord-factor-analogues-biophysical-properties-of-neat-glycolipids-and-nano-self-assemblies-with-dda
#9
Rie S Kallerup, Henrik Franzyk, Mikkel L Schiøth, Sarah Justesen, Birte Martin-Bertelsen, Fabrice Rose, Cecilie M Madsen, Dennis Christensen, Karen S Korsholm, Anan Yaghmur, Camilla Foged
Synthetic mycobacterial cord factor analogues, e.g., trehalose 6,6'-dibehenate (TDB), are highly promising adjuvants due to their strong immunopotentiating capabilities, but their biophysical properties have remained poorly characterized. Here, we report the synthesis of an array of synthetic TDB analogues varying in acyl chain length, degree of acylation and headgroup display, which was subjected to biophysical characterization of neat non-dispersed self-assembled nanostructures in excess buffer and as aqueous dispersions with cationic dimethyldioctadecylammonium (DDA) bromide...
May 12, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28492224/endogenous-adenosine-maintains-cartilage-homeostasis-and-exogenous-adenosine-inhibits-osteoarthritis-progression
#10
Carmen Corciulo, Matin Lendhey, Tuere Wilder, Hanna Schoen, Alexander Samuel Cornelissen, Gregory Chang, Oran D Kennedy, Bruce N Cronstein
Osteoarthritis (OA) is characterized by cartilage destruction and chondrocytes have a central role in this process. With age and inflammation chondrocytes have reduced capacity to synthesize and maintain ATP, a molecule important for cartilage homeostasis. Here we show that concentrations of ATP and adenosine, its metabolite, fall after treatment of mouse chondrocytes and rat tibia explants with IL-1β, an inflammatory mediator thought to participate in OA pathogenesis. Mice lacking A2A adenosine receptor (A2AR) or ecto-5'nucleotidase (an enzyme that converts extracellular AMP to adenosine) develop spontaneous OA and chondrocytes lacking A2AR develop an 'OA phenotype' with increased expression of Mmp13 and Col10a1...
May 11, 2017: Nature Communications
https://www.readbyqxmd.com/read/28487935/folate-receptor-targeted-ultrasonic-pfob-nanoparticles-synthesis-characterization-and-application-in-tumor-targeted-imaging
#11
Keshi Li, Yahui Liu, Shengmin Zhang, Youfeng Xu, Jianshuai Jiang, Fengying Yin, Yue Hu, Baosan Han, Shuxiong Ge, Li Zhang, Yong Wang
In this study, we aimed to determine an effective strategy for the synthesis of folate receptor (FR) targeted-nanoparticles (FRNPs). The nanoparticles used as ultrasound contrast agents (UCAs) were composed of a liquid core of perfluorooctyl bromide (PFOB) liposome and a targeted shell chemically conjugated with folic acid (FA) and polyethylene glycol (PEG). This was done in order to avoid recognition and clearance by the mononuclear phagocyte system [also known as the reticuloendothelial system (RES)] and enhance the targeting capability of the nanoparticles to tumors overexpressing folate receptor (FR)...
June 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28486807/near-infrared-responsive-gold-layersome-nanoshells
#12
Akram Abbasi, Keunhan Park, Arijit Bose, Geoffrey D Bothun
Anionic liposomes coated with the cationic polyelectrolyte poly-l-lysine (PLL), or layersomes, were used as soft, self-assembled templates for synthesizing gold nanoshells that absorb near infrared radiation. The gold-nanoshells were formed using two techniques (a) direct reduction of tetrachloroauric acid on the layersomes, and (b) reduction of a tetrachloroauric acid/potassium carbonate 'growth' solution on nanosized gold seeds bound to the surface of layersomes. The resulting structures were characterized by transmission and scanning electron microscopy, and visible-near infrared spectroscopy...
May 9, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28475934/delivery-of-cationic-quantum-dots-using-fusogenic-liposomes-in-living-cells
#13
Anna L L Matos, Goreti Pereira, Paulo E Cabral Filho, Beate S Santos, Adriana Fontes
Quantum dots (QDs) are fluorescent nanocrystals that present unique optical properties, especially a high photostability. However, their use for intracellular studies is still limited since their passage through the living cell membranes does not occur passively. In this work, we adapted the ethanol injection method to encapsulate cationic hydrophilic QDs into fusogenic liposomes, to deliver them in living cells. Liposomes were characterized using zeta potential, dynamic light scattering (DLS), fluorescence microscopy and transmission electron microscopy (TEM)...
April 23, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28465086/dual-7-ethyl-10-hydroxycamptothecin-conjugated-phospholipid-prodrug-assembled-liposomes-with-in-vitro-anticancer-effects
#14
Yawei Du, Wei Zhang, Ruiyu He, Muhammad Ismail, Longbing Ling, Chen Yao, Zhenglin Fu, Xinsong Li
7-Ethyl-10-hydroxycamptothecin (SN38), as a highly active topoisomerase I inhibitor, is 200-2000-fold more cytotoxic than irinotecan (CPT-11) commercially available as Camptosar®. However, poor solubility and low stability extensively restricted its clinical utility. In this report, dual SN38 phospholipid conjugate (Di-SN38-PC) prodrug based liposomes were developed in order to compact these drawbacks. Di-SN38-PC prodrug was first synthesized by inhomogeneous conjugation of two SN38-20-O-succinic acid molecules with L-α-glycerophosphorylcholine (GPC)...
April 22, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/28462699/pegylated-liposomes-of-meloxicam-optimization-by-quality-by-design-in-vitro-characterization-and-cytotoxicity-evaluation
#15
Jessy Shaji, Ipshita Menon
BACKGROUND: Chemoprevention is a strategy which uses therapeutic agents which are essentially not anti-cancer drugs but, however, impedes the carcinogenesis. Meloxicam (MLX) is one such agent which has the potential to be employed in the chemopreventive therapy of colorectal cancer. However, MLX poses shortcomings with respect to its dose required for cytotoxicity. To improve the formulation, we used Quality by design (QbD) for optimization. QbD is a method that employs quality-improving scientific methods that build quality into the formulation by isolating the factors which affect the critical quality attributes the formulation...
April 28, 2017: Pharmaceutical Nanotechnology
https://www.readbyqxmd.com/read/28461265/preparation-and-characterization-of-isoniazid-loaded-crude-soybean-lecithin-liposomes
#16
Christian Isalomboto Nkanga, Rui Werner Krause, Xavier Siwe Noundou, Roderick Bryan Walker
Tuberculosis (TB) is a poverty related infectious disease that is rapidly giving rise to public health concerns. Lengthy drug administration and frequent adverse side-effects associated with TB treatment make anti-tubercular drugs (ATDs) good candidates for drug delivery studies. This work aimed to formulate and prepare liposomes as a cost-effective option for ATD delivery. Liposomes were prepared by film hydration using crude soybean lecithin (CL) and not pure phospholipids as in the normal practice. Cholesterol was also used (up to 25% mass ratio), and isoniazid (INH) was encapsulated as model drug using a freeze-thaw loading technique...
April 29, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28457130/azide-modified-membrane-lipids-synthesis-properties-and-reactivity
#17
Sindy Lindner, Kai Gruhle, Rico Schmidt, Vasil M Garamus, Daniel Ramsbeck, Gerd Hause, Annette Meister, Andrea Sinz, Simon Drescher
In the present work, we describe the synthesis and the temperature-dependent behavior of photoreactive membrane lipids as well as their capability to study peptide/lipid interactions. The modified phospholipids contain an azide group either in the middle part or at the end of an alkyl chain and also differ in the linkage (ester vs ether) of the second alkyl chain. The temperature-dependent aggregation behavior of the azidolipids was studied using differential scanning calorimetry (DSC), Fourier-transform infrared (FTIR) spectroscopy, and small-angle X-ray scattering (SAXS)...
May 10, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28451782/oligo-and-polypeptide-conjugates-of-cationic-porphyrins-binding-cellular-uptake-and-cellular-localization
#18
Ádám Orosz, Szilvai Bősze, Gábor Mező, Ildikó Szabó, Levente Herényi, Gabriella Csík
Recently, we have characterized the DNA and nucleoprotein (NP) binding of bis(4-N-methylpyridyl)-15,20-di(4-carboxyphenyl)porphyrin (BMPCP) and meso-tri(4-N-methylpyridyl)-mono(4-carboxyphenyl)porphyrin (TMPCP) and their tetrapeptide conjugates (BMPCP-4P2 and TMPCP-4P, respectively). In this work, we investigated the interaction of TMPCP conjugated to the tetrapeptide branches of branched chain polymeric polypeptide with poly-L-lysine backbone (AK) with DNA or NP using spectroscopic methods. Analysis of absorption spectra revealed the external binding but no intercalation of TMPCP-AK to DNA...
April 27, 2017: Amino Acids
https://www.readbyqxmd.com/read/28450130/from-gene-to-function-cell-free-electrophysiological-and-optical-analysis-of-ion-pumps-in-nanodiscs
#19
Erik Henrich, Janina Sörmann, Peter Eberhardt, Oliver Peetz, Julija Mezhyrova, Nina Morgner, Klaus Fendler, Volker Dötsch, Josef Wachtveitl, Frank Bernhard, Christian Bamann
Nanodiscs that hold a lipid bilayer surrounded by a boundary of scaffold proteins have emerged as a powerful tool for membrane protein solubilization and analysis. By combining nanodiscs and cell-free expression technologies, even completely detergent-free membrane protein characterization protocols can be designed. Nanodiscs are compatible with various techniques, and due to their bilayer environment and increased stability, they are often superior to detergent micelles or liposomes for membrane protein solubilization...
April 24, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28447868/doxorubicin-loaded-liposomes-enhancing-the-oral-bioavailability-by-modulation-of-physicochemical-characteristics
#20
Marjan Daeihamed, Azadeh Haeri, Seyed Nasser Ostad, Masoud Faghih Akhlaghi, Simin Dadashzadeh
AIM: In this study, the effects of liposome characteristics on oral absorption of doxorubicin, as a hydrophilic low-permeability drug, were investigated. MATERIALS & METHODS: Different doxorubicin-loaded liposomes were prepared, characterized and orally administered to 18 groups of rats. Plasma concentrations of doxorubicin and its aglycone metabolite were measured, and Caco-2 uptake and transport of optimum liposomes were investigated. RESULTS: After studying different factors, a fourfold increase in oral bioavailability was achieved with the non-PEGylated, 120-nm-sized positively charged rigid liposomes (lipid to drug ratio = 10)...
April 27, 2017: Nanomedicine
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