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https://www.readbyqxmd.com/read/28719908/ultrasound-guided-intrauterine-labeling-of-rat-fetuses
#1
Hatice Gulcin Gumus, Miriam Illa, Laura Pla, Anna Gonzalez, Elena Demicheva, David Ley, Fatima Crispi, Eduard Gratacos
AIM: To compare intra-amniotic versus fetal subcutaneous injections for selective fetal labeling in multifetal rat pregnancies. METHODS: A total of 14 pregnant rats were randomized to receive intra-amniotic injections of dyes (including Fluorescein, Indigo Carmine, or Evans Blue) or fetal subcutaneous injections (of commercial tattoo ink) both guided by ultrasound at 15-17 days of gestation. Survival, injection, and labeling success rates of both techniques were compared...
July 18, 2017: Gynecologic and Obstetric Investigation
https://www.readbyqxmd.com/read/28719437/a-12-day-old-male-with-a-skin-tag-on-the-chin
#2
Syed M Abedi, Freya Van Driessche, Jaime Alexander, Edward Heilman, Sharon Glick
No abstract text is available yet for this article.
July 14, 2017: American Journal of Dermatopathology
https://www.readbyqxmd.com/read/28718301/web-addiction-in-the-brain-cortical-oscillations-autonomic-activity-and-behavioral-measures
#3
Michela Balconi, Salvatore Campanella, Roberta Finocchiaro
Background and aims Internet addiction (IA) was recently defined as a disorder tagging both the impulse control and the reward systems. Specifically, inhibitory deficits and reward bias were considered highly relevant in IA. This research aims to examine the electrophysiological correlates and autonomic activity [skin conductance response (SCR) and heart rate] in two groups of young subjects (N = 25), with high or low IA profile [tested by the Internet Addiction Test (IAT)], with specific reference to gambling behavior...
July 18, 2017: Journal of Behavioral Addictions
https://www.readbyqxmd.com/read/28713775/salivary-tick-cystatin-omc2-targets-lysosomal-cathepsins-s-and-c-in-human-dendritic-cells
#4
Tina Zavašnik-Bergant, Robert Vidmar, Andreja Sekirnik, Marko Fonović, Jiří Salát, Lenka Grunclová, Petr Kopáček, Boris Turk
To ensure successful feeding tick saliva contains a number of inhibitory proteins that interfere with the host immune response and help to create a permissive environment for pathogen transmission. Among the potential targets of the salivary cystatins are two host cysteine proteases, cathepsin S, which is essential for antigen- and invariant chain-processing, and cathepsin C (dipeptidyl peptidase 1, DPP1), which plays a critical role in processing and activation of the granule serine proteases. Here, the effect of salivary cystatin OmC2 from Ornithodoros moubata was studied using differentiated MUTZ-3 cells as a model of immature dendritic cells of the host skin...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28713399/metabolite-profiling-reveals-developmental-inequalities-in-pinot-noir-berry-tissues-late-in-ripening
#5
Amanda M Vondras, Mauro Commisso, Flavia Guzzo, Laurent G Deluc
Uneven ripening in Vitis vinifera is increasingly recognized as a phenomenon of interest, with substantial implications for fruit and wine composition and quality. This study sought to determine whether variation late in ripening (∼Modified Eichhorn-Lorenz stage 39) was associated with developmental differences that were observable as fruits within a cluster initiated ripening (véraison). Four developmentally distinct ripening classes of berries were tagged at cluster véraison, sampled at three times late in ripening, and subjected to untargeted HPLC-MS to measure variation in amino acids, sugars, organic acids, and phenolic metabolites in skin, pulp, and seed tissues separately...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28707961/encephalocraniocutaneous-lipomatosis-a-case-report-with-review-of-literature
#6
Shaista Siddiqui, Shazia Naaz, Mehtab Ahmad, Zafar Ahmad Khan, Shagufta Wahab, Basmah Abdur Rashid
Encephalocraniocutaneous lipomatosis (ECCL) or Haberland syndrome is an uncommon sporadic neurocutaneous syndrome of unknown origin. The rarity and common ignorance of the condition often makes diagnosis difficult. The hallmark of this syndrome is the triad of skin, ocular and central nervous system (CNS) involvement and includes a long list of combination of conditions. Herein we report a case of a 5-month-old male child who presented to our centre with complaint of seizure. The patient had various cutaneous and ocular stigmatas of the disease in the form of patchy alopecia of the scalp, right-sided limbal dermoid and a nodular skin tag near the lateral canthus of the right eye...
January 1, 2017: Neuroradiology Journal
https://www.readbyqxmd.com/read/28701426/dynamics-of-in-vivo-asc-speck-formation
#7
Paola Kuri, Nicole L Schieber, Thomas Thumberger, Joachim Wittbrodt, Yannick Schwab, Maria Leptin
Activated danger or pathogen sensors trigger assembly of the inflammasome adaptor ASC into specks, large signaling platforms considered hallmarks of inflammasome activation. Because a lack of in vivo tools has prevented the study of endogenous ASC dynamics, we generated a live ASC reporter through CRISPR/Cas9 tagging of the endogenous gene in zebrafish. We see strong ASC expression in the skin and other epithelia that act as barriers to insult. A toxic stimulus triggered speck formation and rapid pyroptosis in keratinocytes in vivo...
July 12, 2017: Journal of Cell Biology
https://www.readbyqxmd.com/read/28698740/the-correlation-between-body-mass-index-with-the-occurrence-of-skin-tag
#8
Nelva K Jusuf, Imam B Putra, Johan Kartayana
BACKGROUND: Skin tag is a benign tumour of the skin with a soft consistency which commonly occurs in the flexure area. Skin tag often found in individuals with middle and old age. Until now, there are many of theories have been proposed to explain the mechanism of the skin tag, one of the theory is obesity. AIM: To determine the body mass index (BMI) in patients with skin tag. METHODS: This study is an analytic study with cross-sectional designs involving 32 subjects with skin tag and 32 controls...
June 15, 2017: Open Access Macedonian Journal of Medical Sciences
https://www.readbyqxmd.com/read/28647779/transcriptome-profiling-and-digital-gene-expression-analysis-of-the-skin-of-dybowski-s-frog-rana-dybowskii-exposed-to-aeromonas-hydrophila
#9
Yi-Gang Xu, Long-Hui Chai, Wen Shi, Dan-Dan Wang, Jing-Yu Zhang, Xiang-Hong Xiao
Recently, populations of Rana dybowskii, an important amphibian species in Northeast China, have decreased, mainly owing to the disease caused by Aeromonas hydrophila. However, effective control methods have not yet been developed. In order to explore the immune responses of R. dybowskii upon exposure to A. hydrophila infection, Illumina high-throughput transcriptome sequencing and digital gene expression (DGE) technology were employed to investigate transcriptomic changes in the skin of R. dybowskii exposed to A...
June 24, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28640899/normative-3d-opto-electronic-stereo-photogrammetric-posture-and-spine-morphology-data-in-young-healthy-adult-population
#10
Moreno D'Amico, Edyta Kinel, Piero Roncoletta
Observational cross-sectional study. The current study aims to yield normative data: i.e., the physiological standard for 30 selected quantitative 3D parameters that accurately capture and describe a full-skeleton, upright-standing attitude. Specific and exclusive consideration was given to three distinct categories: postural, spine morphology and pelvic parameters. To capture such 3D parameters, the authors selected a non-ionising 3D opto-electronic stereo-photogrammetric approach. This required the identification and measurement of 27 body landmarks, each specifically tagged with a skin marker...
2017: PloS One
https://www.readbyqxmd.com/read/28607792/a-constellation-of-rare-findings-in-a-case-of-goldenhar-syndrome
#11
Mitesh Bedi, Rakesh Kumar Jain, Vipin Kumar Barala, Abhimanyu Singh, Hiranmayi Jha
An 18-month-old child presented with right macrostomia, bilateral preauricular skin tags, bilateral CTEV, squint in bilateral eyes, thoracic vertebral anomalies, right sided aortic arch, and associated left pulmonary agenesis. The patient did not have any associated respiratory symptoms. Ipsilateral pulmonary agenesis is considered as a rare association with Goldenhar syndrome and a case of contralateral pulmonary aplasia has been described as an even rarer association.
2017: Case Reports in Pediatrics
https://www.readbyqxmd.com/read/28601875/activities-of-mscs-derived-from-transgenic-mice-seeded-on-adm-scaffolds-in-wound-healing-and-assessment-by-advanced-optical-techniques
#12
Ning Wang, Hanping Liu, Xuefeng Li, Qilei Zhang, Maosheng Chen, Ying Jin, Xiaoyuan Deng
BACKGROUND/AIMS: Bone marrow Mesenchymal stem cells (MSCs) are promising for promoting cutaneous wound healing through reinforcing cellular processes. We evaluated the effect of GFP-tagged MSCs transplantation on skin regeneration in excisional wounds in mice. METHODS: MSCs from GFP-labeled transgenic mice were co-cultured with acellular dermal matrix (ADM) scaffolds, and MSC-ADM scaffolds were transplanted into surgical skin wounds of BALB/c mice. After implantation, the survival and behavior of MSCs were examined by second harmonic generation and two-photon excitation fluorescence imaging, western blotting and DNA amplification and sequencing...
2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28567799/cox-2-selective-inhibitors-celecoxib-and-deracoxib-modulate-trpv3-channels
#13
Stefan Spyra, Anne Meisner, Michael Schaefer, Kerstin Hill
BACKGROUND AND PURPOSE: The transient receptor potential vanilloid 3 channel (TRPV3) is a heat-sensitive ion channel which is predominantly expressed in keratinocytes. TRPV3 is involved in multiple physiological and pathophysiological processes within the skin, including cutaneous nociception, temperature sensation, and the development of itch. The role of TRPV3 in such processes is only poorly understood, therefore, the establishment of selective modulators of TRPV3 channels is highly desirable...
May 31, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/28476590/automating-mouse-weighing-in-group-homecages-with-raspberry-pi-micro-computers
#14
Omid Noorshams, Jamie D Boyd, Timothy H Murphy
BACKGROUND: Operant training systems make use of water or food restriction and make it necessary to weigh animals to ensure compliance with experimental endpoints. In other applications periodic weighing is necessary to assess drug side-effects, or as an endpoint in feeding experiments. Periodic weighing while essential can disrupt animal circadian rhythms and social structure. NEW METHOD: Automatic weighing system within paired mouse homecages. Up to 10 mice freely move between two cages (28×18×9cm) which were connected by a weighing chamber mounted on a load cell...
June 15, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28461902/effect-of-skin-dielectric-properties-on-the-read-range-of-epidermal-ultra-high-frequency-radio-frequency-identification-tags
#15
Dumtoochukwu O Oyeka, John C Batchelor, Ali Mohamad Ziai
This Letter presents an investigation of the effect of human tissue conductivity and permittivity on the performance of epidermal transfer tattoo ultra-high frequency radio-frequency identification (RFID) tags. The measurements were carried out on 20 individuals and the variations in the measured dielectric properties correlate well with variations in the measured tag read range on the individuals and to a lesser extent with their body mass index values. Simulation results also showed the effects of permittivity and conductivity on the designed resonance frequency of the RFID tag...
April 2017: Healthcare Technology Letters
https://www.readbyqxmd.com/read/28445980/the-pp4r1-sub-unit-of-protein-phosphatase-pp4-is-essential-for-inhibition-of-nf-%C3%AE%C2%BAb-by-merkel-polyomavirus-small-tumour-antigen
#16
Hussein Abdul-Sada, Marietta Müller, Rajni Mehta, Rachel Toth, J Simon C Arthur, Adrian Whitehouse, Andrew Macdonald
Merkel cell carcinoma (MCC) is a highly aggressive skin cancer with a high metastatic potential. The majority of MCC cases are caused by the Merkel cell polyomavirus (MCPyV), through expression of the virus-encoded tumour antigens. Whilst mechanisms attributing tumour antigen expression to transformation are being uncovered, little is known of the mechanisms by which MCPyV persists in the host. We previously identified the MCPyV small T antigen (tAg) as a novel inhibitor of nuclear factor kappa B (NF-kB) signalling and a modulator of the host anti-viral response...
April 11, 2017: Oncotarget
https://www.readbyqxmd.com/read/28398356/snp-based-hla-allele-tagging-imputation-and-association-with-antiepileptic-drug-induced-cutaneous-reactions-in-hong-kong-han-chinese
#17
H Gui, M Kwok, L Baum, P C Sham, P Kwan, S S Cherny
Human leukocyte antigen (HLA) genes control the regulation of the human immune system and are involved in immune-related diseases. Population surveys on relationships between single nucleotide polymorphisms (SNP) and HLA alleles are essential to conduct genetic association between HLA variants and diseases. Samples were obtained from our in-house database for epilepsy genetics and pharmacogenetics research. Using 184 epilepsy patients with both genome-wide SNP array and HLA-A/B candidate gene sequencing data, we sought tagging SNPs that completely represent sixHLA risk alleles; in addition, a Hong Kong population-specific reference panel was constructed for SNP-based HLA imputation...
April 11, 2017: Pharmacogenomics Journal
https://www.readbyqxmd.com/read/28397982/generation-of-chimeric-minipigs-by-aggregating-4-to-8-cell-stage-blastomeres-from-somatic-cell-nuclear-transfer-with-the-tracing-of-enhanced-green-fluorescent-protein
#18
Huili Ji, Chuan Long, Chong Feng, Ningning Shi, Yingdi Jiang, Guomin Zeng, Xirui Li, Jingjing Wu, Lin Lu, Shengsheng Lu, Dengke Pan
BACKGROUND: Blastocyst complementation is an important technique for generating chimeric organs in organ-deficient pigs, which holds great promise for solving the problem of a shortage of organs for human transplantation procedures. Porcine chimeras have been generated using embryonic germ cells, embryonic stem cells, and induced pluripotent stem cells; however, there are no authentic pluripotent stem cells for pigs. In previous studies, blastomeres from 4- to 8-cell-stage parthenogenetic embryos were able to generate chimeric fetuses efficiently, but the resulting fetuses did not produce live-born young...
April 11, 2017: Xenotransplantation
https://www.readbyqxmd.com/read/28371113/simulated-sunlight-mediated-photodynamic-therapy-for-melanoma-skin-cancer-by-titanium-dioxide-nanoparticle-gold-nanocluster-graphene-heterogeneous-nanocomposites
#19
Yan Cheng, Yun Chang, Yanlin Feng, Ning Liu, Xiujuan Sun, Yuqing Feng, Xi Li, Haiyuan Zhang
Simulated sunlight has promise as a light source able to alleviate the severe pain associated with patients during photodynamic therapy (PDT); however, low sunlight utilization efficiency of traditional photosensitizers dramatically limits its application. Titanium-dioxide-nanoparticle-gold-nanocluster-graphene (TAG) heterogeneous nanocomposites are designed to efficiently utilize simulated sunlight for melanoma skin cancer PDT. The narrow band gap in gold nanoclusters (Au NCs), and staggered energy bands between Au NCs, titanium dioxide nanoparticles (TiO2 NPs), and graphene can result in efficient utilization of simulated sunlight and separation of electron-hole pairs, facilitating the production of abundant hydroxyl and superoxide radicals...
March 31, 2017: Small
https://www.readbyqxmd.com/read/28368887/uptake-of-an-acrochordon-incidentally-detected-on-68ga-prostate-specific-membrane-antigen-pet-ct
#20
Gozde Daglioz Gorur, Turkay Hekimsoy, Serkan Isgoren, Aysun Sikar Akturk, Hakan Demir
Ga prostate-specific membrane antigen (PSMA) PET/CT is a promising tool for imaging of prostate cancer. Ga-PSMA PET/CT uptake of prostate cancer and its metastases are reflective of significant overexpression of PSMA. However, PSMA expression of benign neoplasms and nonprostate epithelial malignancies is not very well defined. We report a moderate Ga-PSMA uptake of an acrochordon (skin tag), which was incidentally found in a patient referred for staging prostate cancer. Acrochordon is a frequent, small, soft, skin-colored or hyperpigmented, benign, and usually pedunculated neoplasm of the skin...
June 2017: Clinical Nuclear Medicine
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