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gene pyramiding in wheat breeding

Dhouha Kthiri, Alexander Loladze, P R MacLachlan, Amidou N'Diaye, Sean Walkowiak, Kirby Nilsen, Susanne Dreisigacker, Karim Ammar, Curtis J Pozniak
Widening the genetic basis of leaf rust resistance is a primary objective of the global durum wheat breeding effort at the International Wheat and Maize Improvement Center (CIMMYT). Breeding programs in North America are following suit, especially after the emergence of new races of Puccinia triticina such as BBG/BP and BBBQD in Mexico and the United States, respectively. This study was conducted to characterize and map previously undescribed genes for leaf rust resistance in durum wheat and to develop reliable molecular markers for marker-assisted breeding...
2018: PloS One
Jianhui Wu, Qingdong Zeng, Qilin Wang, Shengjie Liu, Shizhou Yu, Jingmei Mu, Shuo Huang, Hanan Sela, Assaf Distelfeld, Lili Huang, Dejun Han, Zhensheng Kang
NGS-assisted super pooling emerging as powerful tool to accelerate gene mapping and haplotype association analysis within target region uncovering specific linkage SNPs or alleles for marker-assisted gene pyramiding. Conventional gene mapping methods to identify genes associated with important agronomic traits require significant amounts of financial support and time. Here, a single nucleotide polymorphism (SNP)-based mapping approach, RNA-Seq and SNP array assisted super pooling analysis, was used for rapid mining of a candidate genomic region for stripe rust resistance gene Yr26 that has been widely used in wheat breeding programs in China...
April 17, 2018: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
Stefan Böhmdorfer, Josua Timotheus Oberlerchner, Christina Fuchs, Thomas Rosenau, Heinrich Grausgruber
Background: Anthocyanins are abundant secondary metabolites responsible for most blue to blue-black, and red to purple colors of various plant organs. In wheat grains, anthocyanins are accumulated in the pericarp and/or aleurone layer. Anthocyanin pigmented wheat grains can be processed into functional foods with potential health benefits due to the antioxidant properties of the anthocyanins. The grain anthocyanin content can be increased by pyramidizing the different genes responsible for the accumulation of anthocyanins in the different grain layers...
2018: Plant Methods
Jindong Liu, Zhonghu He, Awais Rasheed, Weie Wen, Jun Yan, Pingzhi Zhang, Yingxiu Wan, Yong Zhang, Chaojie Xie, Xianchun Xia
BACKGROUND: Black point is a serious threat to wheat production and can be managed by host resistance. Marker-assisted selection (MAS) has the potential to accelerate genetic improvement of black point resistance in wheat breeding. We performed a genome-wide association study (GWAS) using the high-density wheat 90 K and 660 K single nucleotide polymorphism (SNP) assays to better understand the genetic basis of black point resistance and identify associated molecular markers. RESULTS: Black point reactions were evaluated in 166 elite wheat cultivars in five environments...
November 23, 2017: BMC Plant Biology
Firdissa E Bokore, Richard D Cuthbert, Ron E Knox, Harpinder S Randhawa, Colin W Hiebert, Ron M DePauw, Asheesh K Singh, Arti Singh, Andrew G Sharpe, Amidou N'Diaye, Curtis J Pozniak, Curt McCartney, Yuefeng Ruan, Samia Berraies, Brad Meyer, Catherine Munro, Andy Hay, Karim Ammar, Julio Huerta-Espino, Sridhar Bhavani
Quantitative trait loci controlling stripe rust resistance were identified in adapted Canadian spring wheat cultivars providing opportunity for breeders to stack loci using marker-assisted breeding. Stripe rust or yellow rust, caused by Puccinia striiformis Westend. f. sp. tritici Erikss., is a devastating disease of common wheat (Triticum aestivum L.) in many regions of the world. The objectives of this research were to identify and map quantitative trait loci (QTL) associated with stripe rust resistance in adapted Canadian spring wheat cultivars that are effective globally, and investigate opportunities for stacking resistance...
December 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
Ali Aliakbari Sadeghabad, Ali Dadkhodaie, Bahram Heidari, Hooman Razi, Reza Mostowfizadeh-Ghalamfarsa
Leaf rust, caused by Puccinia triticina, is a common wheat disease worldwide. Developing resistant cultivars through deploying new or pyramiding resistance genes in a suitable line, is the most effective approach to control this disease. However, to stack genes in a genotype, efficient and reliable markers are required. In the present study, F2 plants and their corresponding F3 families from a cross between the resistant line; Thatcher (Tc) Lr18, and the susceptible cultivar 'Boolani' were used to map rust resistance gene, Lr18 using SSR markers on chromosome 5BL of hexaploid wheat...
March 2017: Breeding Science
Mulualem T Kassa, Frank M You, Colin W Hiebert, Curtis J Pozniak, Pierre R Fobert, Andrew G Sharpe, James G Menzies, D Gavin Humphreys, Nicole Rezac Harrison, John P Fellers, Brent D McCallum, Curt A McCartney
BACKGROUND: Lr16 is a widely deployed leaf rust resistance gene in wheat (Triticum aestivum L.) that is highly effective against the North American Puccinia triticina population when pyramided with the gene Lr34. Lr16 is a seedling leaf rust resistance gene conditioning an incompatible interaction with a distinct necrotic ring surrounding the uredinium. Lr16 was previously mapped to the telomeric region of the short arm of wheat chromosome 2B. The goals of this study were to develop numerous single nucleotide polymorphism (SNP) markers for the Lr16 region and identify diagnostic gene-specific SNP marker assays for marker-assisted selection (MAS)...
February 15, 2017: BMC Plant Biology
Genqiao Li, Guihua Bai, Brett F Carver, Norman C Elliott, Rebecca S Bennett, Yanqi Wu, Robert Hunger, J Michael Bonman, Xiangyang Xu
Eight QTL for coleoptile length were identified in a genome-wide association study on a set of 893 wheat accessions, four of which are novel loci. Wheat cultivars with long coleoptiles are preferred in wheat-growing regions where deep planting is practiced. However, the wide use of gibberellic acid (GA)-insensitive dwarfing genes, Rht-B1b and Rht-D1b, makes it challenging to breed dwarf wheat cultivars with long coleoptiles. To understand the genetic basis of coleoptile length, we performed a genome-wide association study on a set of 893 landraces and historical cultivars using 5011 single nucleotide polymorphism (SNP) markers...
February 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
Meng Lin, Dadong Zhang, Shubing Liu, Guorong Zhang, Jianming Yu, Allan K Fritz, Guihua Bai
BACKGROUND: Pre-harvest sprouting (PHS) in wheat can cause substantial reduction in grain yield and end-use quality. Grain color (GC) together with other components affect PHS resistance. Several quantitative trait loci (QTL) have been reported for PHS resistance, and two of them on chromosome 3AS (TaPHS1) and 4A have been cloned. METHODS: To determine genetic architecture of PHS and GC and genetic relationships of the two traits, a genome-wide association study (GWAS) was conducted by evaluating a panel of 185 U...
October 12, 2016: BMC Genomics
N Ali, Js Pat Heslop-Harrison, H Ahmad, R A Graybosch, G L Hein, T Schwarzacher
Pyramiding of alien-derived Wheat streak mosaic virus (WSMV) resistance and resistance enhancing genes in wheat is a cost-effective and environmentally safe strategy for disease control. PCR-based markers and cytogenetic analysis with genomic in situ hybridisation were applied to identify alien chromatin in four genetically diverse populations of wheat (Triticum aestivum) lines incorporating chromosome segments from Thinopyrum intermedium and Secale cereale (rye). Out of 20 experimental lines, 10 carried Th...
August 2016: Heredity
Quddoos H Muqaddasi, Ulrike Lohwasser, Manuela Nagel, Andreas Börner, Klaus Pillen, Marion S Röder
In a number of crop species hybrids are able to outperform line varieties. The anthers of the autogamous bread wheat plant are normally extruded post anthesis, a trait which is unfavourable for the production of F1 hybrid grain. Higher anther extrusion (AE) promotes cross fertilization for more efficient hybrid seed production. Therefore, this study aimed at the genetic dissection of AE by genome wide association studies (GWAS) and determination of the main effect QTL. We applied GWAS approach to identify DArT markers potentially linked to AE to unfold its genetic basis in a panel of spring wheat accessions...
2016: PloS One
Burkhard Steuernagel, Sambasivam K Periyannan, Inmaculada Hernández-Pinzón, Kamil Witek, Matthew N Rouse, Guotai Yu, Asyraf Hatta, Mick Ayliffe, Harbans Bariana, Jonathan D G Jones, Evans S Lagudah, Brande B H Wulff
Wild relatives of domesticated crop species harbor multiple, diverse, disease resistance (R) genes that could be used to engineer sustainable disease control. However, breeding R genes into crop lines often requires long breeding timelines of 5-15 years to break linkage between R genes and deleterious alleles (linkage drag). Further, when R genes are bred one at a time into crop lines, the protection that they confer is often overcome within a few seasons by pathogen evolution. If several cloned R genes were available, it would be possible to pyramid R genes in a crop, which might provide more durable resistance...
June 2016: Nature Biotechnology
Jun Guo, Xiuli Zhang, Yanlin Hou, Jinjin Cai, Xiaorong Shen, Tingting Zhou, Huihui Xu, Herbert W Ohm, Hongwei Wang, Anfei Li, Fangpu Han, Honggang Wang, Lingrang Kong
Wheat lines with shortened Th. ponticum chromatin carrying Fhb7 and molecular markers linked to Fhb7 will accelerate the transfer of Fhb7 to breeding lines and provide an important resource for future map-based cloning of this gene. Fusarium head blight is a major wheat disease globally. A major FHB resistance gene, designated as Fhb7, derived from Thinopyrum ponticum, was earlier transferred to common wheat, but was not used in wheat breeding due to linkage drag. The aims of this study were to (1) saturate this FHB resistance gene region; (2) develop and characterize secondary translocation lines with shortened Thinopyrum segments carrying Fhb7 using ph1b; (3) pyramid Fhb7 and Fhb1 by marker-assisted selection...
November 2015: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
Bin Zhang, Xia Liu, Weina Xu, Jianzhong Chang, Ang Li, Xinguo Mao, Xueyong Zhang, Ruilian Jing
Axillary meristems play an important role in determining final plant architecture and floral structures. Tomato Ls, Arabidopsis LAS and rice MOC1 are orthologous genes regulating axillary meristem initiation and outgrowth. Their functions are generally conserved but the functional specificities are divergent among species. Obvious differences between rice panicles and wheat spikes suggest the divergent functions of MOC1 and its wheat ortholog. We show that TaMOC1 might be involved in wheat spikelet development...
2015: Scientific Reports
Joey C Cainong, William W Bockus, Yigao Feng, Peidu Chen, Lili Qi, Sunish K Sehgal, Tatiana V Danilova, Dal-Hoe Koo, Bernd Friebe, Bikram S Gill
This manuscript describes the transfer and molecular cytogenetic characterization of a novel source of Fusarium head blight resistance in wheat. Fusarium head blight (FHB) caused by the fungus Fusarium graminearum Schwabe [telomorph = Gibberella zeae (Schwein. Fr.) Petch] is an important disease of bread wheat, Triticum aestivum L. (2n = 6x = 42, AABBDD) worldwide. Wheat has limited resistance to FHB controlled by many loci and new sources of resistance are urgently needed. The perennial grass Elymus tsukushiensis thrives in the warm and humid regions of China and Japan and is immune to FHB...
June 2015: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
Yuanfeng Hao, Ryan Parks, Christina Cowger, Zhenbang Chen, Yingying Wang, Dan Bland, J Paul Murphy, Mohammed Guedira, Gina Brown-Guedira, Jerry Johnson
A new powdery mildew resistance gene Pm54 was identified on chromosome 6BL in soft red winter wheat. Powdery mildew is causing increasing damage to wheat production in the southeastern USA. To combat the disease, a continuing need exists to discover new genes for powdery mildew resistance and to incorporate those genes into breeding programs. Pioneer(®) variety 26R61 (shortened as 26R61) and AGS 2000 have been used as checks in the Uniform Southern Soft Red Winter Wheat Nursery for a decade, and both have provided good resistance across regions during that time...
March 2015: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
Jianmin Zheng, Zehong Yan, Li Zhao, Shizhao Li, Zengyan Zhang, Rosewarne Garry, Wuyun Yang, Zongjun Pu
Stripe rust, a major disease in areas where cool temperatures prevail, can strongly influence grain yield. To control this disease, breeders have incorporated seedling resistance genes from a variety of sources outside the primary wheat gene pool. The wheat line C51, introduced from the International Center for Agricultural Research in the Dry Areas (ICARDA), Syria, confers resistance to all races of Puccinia striiformis f. sp. tritici (PST) in China. To map the resistant gene(s) against stripe rust in wheat line C51, 212 F8 recombinant inbred lines (RILs) derived from the cross X440 x C51 were inoculated with Chinese PST race CYR33 (Chinese yellow rust, CYR) in the greenhouse...
August 2014: Journal of Genetics
X L Zhou, D J Han, X M Chen, H L Gou, S J Guo, L Rong, Q L Wang, L L Huang, Z S Kang
We report a new stripe rust resistance gene on chromosome 7AS in wheat and molecular markers useful for transferring it to other wheat genotypes. Several new races of the stripe rust pathogen have established throughout the wheat growing regions of China in recent years. These new races are virulent to most of the designated seedling resistance genes limiting the resistance sources. It is necessary to identify new genes for diversification and for pyramiding different resistance genes in order to achieve more durable resistance...
November 2014: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
Daniel Stirnweis, Samira D Milani, Susanne Brunner, Gerhard Herren, Gabriele Buchmann, David Peditto, Tina Jordan, Beat Keller
The development of high-yielding varieties with broad-spectrum durable disease resistance is the ultimate goal of crop breeding. In plants, immune receptors of the nucleotide-binding-leucine-rich repeat (NB-LRR) class mediate race-specific resistance against pathogen attack. When employed in agriculture this type of resistance is often rapidly overcome by newly adapted pathogen races. The stacking of different resistance genes or alleles in F1 hybrids or in pyramided lines is a promising strategy for achieving more durable resistance...
September 2014: Plant Journal: for Cell and Molecular Biology
Urmil Bansal, Harbans Bariana, Debbie Wong, Mandeep Randhawa, Thomas Wicker, Matthew Hayden, Beat Keller
This article covers detailed characterization and naming of QSr.sun - 5BL as Sr56 . Molecular markers linked with adult plant stem rust resistance gene Sr56 were identified and validated for marker-assisted selection. The identification of new sources of adult plant resistance (APR) and effective combinations of major and minor genes is well appreciated in breeding for durable rust resistance in wheat. A QTL, QSr.sun-5BL, contributed by winter wheat cultivar Arina providing 12-15 % reduction in stem rust severity, was reported in an Arina/Forno recombinant inbred line (RIL) population...
June 2014: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
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