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JOURNAL ARTICLE
R D Simoni, M K Shallenberger
Active transport of amino acids in isolated membrane vesicles of E. coli ML 308-225 is stimulated by oxidation of D-lactate, and this stimulation is dependent on electron transport [Kaback, H. R. & Milner, L. S. (1970) Proc. Nat. Acad. Sci. USA 66, 1008]. In attempting to relate these results to amino-acid transport in intact cells, we isolated mutants of E. coli ML 308-225 that contain defects in D-lactate dehydrogenase (EC 1.1.2.4) and electron transport. Intact cells of these mutants are normal for transport of proline and alanine...
September 1972: Proceedings of the National Academy of Sciences of the United States of America
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