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https://www.readbyqxmd.com/read/29150608/rapid-neo-sex-chromosome-evolution-and-incipient-speciation-in-a-major-forest-pest
#1
Ryan R Bracewell, Barbara J Bentz, Brian T Sullivan, Jeffrey M Good
Genome evolution is predicted to be rapid following the establishment of new (neo) sex chromosomes, but it is not known if neo-sex chromosome evolution plays an important role in speciation. Here we combine extensive crossing experiments with population and functional genomic data to examine neo-XY chromosome evolution and incipient speciation in the mountain pine beetle. We find a broad continuum of intrinsic incompatibilities in hybrid males that increase in strength with geographic distance between reproductively isolated populations...
November 17, 2017: Nature Communications
https://www.readbyqxmd.com/read/29150517/multi-omics-reveal-the-lifestyle-of-the-acidophilic-mineral-oxidizing-model-species-leptospirillum-ferriphilum-t
#2
Stephan Christel, Malte Herold, Sören Bellenberg, Mohamed El Hajjami, Antoine Buetti-Dinh, Igor V Pivkin, Wolfgang Sand, Paul Wilmes, Ansgar Poetsch, Mark Dopson
Leptospirillum ferriphilum plays a major role in acidic, metal rich environments where it represents one of the most prevalent iron oxidizers. These milieus include acid rock and mine drainage as well as biomining operations. Despite its perceived importance, no complete genome sequence of this model species' type strain is available, limiting the possibilities to investigate the strategies and adaptations Leptospirillum ferriphilum(T) applies to survive and compete in its niche. This study presents a complete, circular genome of Leptospirillum ferriphilum(T) DSM 14647 obtained by PacBio SMRT long read sequencing for use as a high quality reference...
November 17, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29150514/sinorhizobium-meliloti-glutathione-reductase-is-required-for-both-redox-homeostasis-and-symbiosis
#3
Guirong Tang, Ningning Li, Yumin Liu, Liangliang Yu, Junhui Yan, Li Luo
Glutathione (L-γ-glutamyl-L-cysteinylglycine; GSH), one of the key antioxidants in Sinorhizobium meliloti, is required for the development of alfalfa (Medicago sativa) nitrogen-fixing nodules. Glutathione exists either in a reduced form (GSH) or an oxidized form (GSSG), and its content is regulated by two pathways in S. meliloti The first pathway is the de novo synthesis of glutathione from its constituent amino acids, namely, Glu, Cys, and Gly, catalyzed by γ-glutamyl cysteine synthetase (GshA) and glutathione synthetase (GshB)...
November 17, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29150315/surgical-options-for-osteoporotic-vertebral-compression-fractures-complicated-with-spinal-deformity-and-neurologic-deficit
#4
Kalliopi Alpantaki, Michael Dohm, Panagiotis Korovessis, Alexander G Hadjipavlou
INTRODUCTION: This paper describes surgical options for Osteoporotic vertebral compression fracture (OVCF) with acute flexible or chronic rigid kyphosis, and pseudarthrosis complicated with pain and neurologic deficit. METHODS: This study has two components. a) A prospective clinical study of surgical treatment of 31 patients (age: 69±11 years) with either acute flexible or progressive pseudarthrotic kyphosis manifested with severe pain or neurological deficit between 2010 and 2014...
November 12, 2017: Injury
https://www.readbyqxmd.com/read/29150010/androgen-deprivation-alters-attention-to-sexually-provocative-visual-stimuli-in-elderly-men
#5
Jaime L Palmer-Hague, Vivian Tsang, Charlenn Skead, Richard J Wassersug, Eleni Nasiopoulos, Alan Kingstone
INTRODUCTION: Testosterone is known to regulate male sexual interest, but the exact way that androgens influence men's sexual cognition remains unclear. AIM: To investigate the influence of androgen deprivation (AD) on visual responses to sexually suggestive stimuli in men treated for prostate cancer with AD therapy. METHODS: Patients with AD-treated prostate cancer, patients with prostate cancer not on AD therapy, and age-matched healthy control participants were exposed to images of male and female runway models fully or minimally clothed...
December 2017: Sexual Medicine
https://www.readbyqxmd.com/read/29149730/divergence-of-insulin-superfamily-ligands-receptors-and-igf-binding-proteins-in-marine-versus-freshwater-stickleback-evidence-of-selection-in-known-and-novel-genes
#6
Tim Pellissier, Hend Al Nafea, Sara V Good
Three-spine stickleback (Gasterosteus aculeatus) is a teleost model for understanding genetic, physiological and morphological changes accompanying freshwater (FW) adaptation. There is growing evidence that the insulin superfamily plays important roles in traits involved in marine and FW adaptation. We performed a candidate gene analysis to look for evidence of selection on 33 insulin superfamily ligand-receptor genes and insulin-like growth factor binding proteins (Igfbp's) in stickleback. Using genotype data from 11 marine and 10 FW populations, we calculated the number of SNPs per site in regulatory and intronic regions, the number of synonymous and nonsynonymous mutations in coding regions, Wright's fixation index (Fst), and performed t-tests to identify SNPs with divergent genotype frequencies between marine/FW versus Atlantic/Pacific populations...
November 10, 2017: Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics
https://www.readbyqxmd.com/read/29149722/effects-of-middle-ear-quasi-static-stiffness-on-sound-transmission-quantified-by-a-novel-3-axis-optical-force-sensor
#7
Ivo Dobrev, Jae Hoon Sim, Baktash Aqtashi, Alexander M Huber, Thomas Linder, Christof Röösli
BACKGROUND: Intra-operative quantification of the ossicle mobility could provide valuable feedback for the current status of the patient's conductive hearing. However, current methods for evaluation of middle ear mobility are mostly limited to the surgeon's subjective impression through manual palpation of the ossicles. This study investigates how middle ear transfer function is affected by stapes quasi-static stiffness of the ossicular chain. The stiffness of the middle ear is induced by a) using a novel fiber-optic 3-axis force sensor to quantify the quasi-static stiffness of the middle ear, and b) by artificial reduction of stapes mobility due to drying of the middle ear...
November 10, 2017: Hearing Research
https://www.readbyqxmd.com/read/29149570/seagrass-mediated-phosphorus-and-iron-solubilisation-in-tropical-sediments
#8
Kasper Elgetti Brodersen, Klaus Koren, Maria Mosshammer, Peter Ralph, Michael Kühl, Jakob Santner
Tropical seagrasses are nutrient limited owing to the strong phosphorus fixation capacity of carbonate-rich sediments, yet they form densely-vegetated, multi-species meadows in oligotrophic tropical waters. Using a novel combination of high-resolution, two-dimensional chemical imaging of O2, pH, iron, sulphide, calcium and phosphorus, we found that tropical seagrasses are able to mobilize the essential nutrients iron and phosphorus in their rhizosphere via multiple biogeochemical pathways. We show that tropical seagrasses mobilize phosphorus and iron within their rhizosphere via plant-induced local acidification, leading to dissolution of carbonates and release of phosphate, and via local stimulation of microbial sulphide production, causing reduction of insoluble Fe(III) oxyhydroxides to dissolved Fe(II) with concomitant phosphate release into the rhizosphere porewater...
November 17, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/29148933/surgical-strategies-for-the-treatment-of-os-odontoideum-with-atlantoaxial-dislocation
#9
Xinghuo Wu, Kirkham B Wood, Yong Gao, Shuai Li, Jing Wang, Ting Ge, Boming Zhao, Zengwu Shao, Shuhua Yang, Cao Yang
OBJECTIVE This study aimed to compare the clinical results of using posterior fixation and fusion with or without anterior decompression to treat os odontoideum with atlantoaxial dislocation. METHODS Twenty-five consecutive patients with os odontoideum were included in this study. Sixteen patients with reducible atlantoaxial dislocation were treated by single-level posterior fusion and stabilization; the other 9 were treated with posterior fusion and stabilization combined with transoral decompression. Pre- and postoperative CT scans and MR images were obtained...
November 17, 2017: Journal of Neurosurgery. Spine
https://www.readbyqxmd.com/read/29148842/erratum
#10
(no author information available yet)
Yao H, Lu H, Zhao H, et al. Open reduction assisted with an external fixator and internal fixation with calcaneal locking plate for intra-articular calcaneal fractures. Foot Ankle Int. 2017;38(10):1107-1114. (Original DOI: 10.1177/1071100717715908) The above article appeared with the incorrect corresponding author. The correct corresponding author is Huading Lu, PhD. The corrected corresponding author information is below: Corresponding Author: Huading Lu, PhD, Department of Orthopaedics, The Fifth Affiliated Hospital of Sun Yat-sen University, NO...
November 1, 2017: Foot & Ankle International
https://www.readbyqxmd.com/read/29147961/spatiotemporal-competition-and-task-relevance-shape-the-spatial-distribution-of-emotional-interference-during-rapid-visual-processing-evidence-from-gaze-contingent-eye-tracking
#11
Briana L Kennedy, Daniel Pearson, David J Sutton, Tom Beesley, Steven B Most
People's ability to perceive rapidly presented targets can be disrupted both by voluntary encoding of a preceding target and by spontaneous attention to salient distractors. Distinctions between these sources of interference can be found when people search for a target in multiple rapid streams instead of a single stream: voluntary encoding of a preceding target often elicits subsequent perceptual lapses across the visual field, whereas spontaneous attention to emotionally salient distractors appears to elicit a spatially localized lapse, giving rise to a theoretical account suggesting that emotional distractors and subsequent targets compete spatiotemporally during rapid serial visual processing...
November 16, 2017: Attention, Perception & Psychophysics
https://www.readbyqxmd.com/read/29147640/robinsoniella-peoriensis-infection-related-to-right-femoral-hardware
#12
Justin Schmetterer, Joseph Gorvetzian, Shaun Yang
Discovered in swine manure relatively recently, Robsinoniella peoriensis is a gram-positive anaerobic bacilli that has been reported to cause human infections. Its antimicrobial susceptibility, epidemiology and overall pathogenicity are still not fully understood. We report a case of a hardware related soft tissue infection in the right femur caused by Robinsoneilla peoriensis following an open reduction internal fixation in a 67 year old immunocompetent woman. The patient was successfully treated with six weeks of ertapenem...
2017: IDCases
https://www.readbyqxmd.com/read/29146773/glyoxal-as-an-alternative-fixative-to-formaldehyde-in-immunostaining-and-super-resolution-microscopy
#13
Katharina N Richter, Natalia H Revelo, Katharina J Seitz, Martin S Helm, Deblina Sarkar, Rebecca S Saleeb, Elisa D'Este, Jessica Eberle, Eva Wagner, Christian Vogl, Diana F Lazaro, Frank Richter, Javier Coy-Vergara, Giovanna Coceano, Edward S Boyden, Rory R Duncan, Stefan W Hell, Marcel A Lauterbach, Stephan E Lehnart, Tobias Moser, Tiago Outeiro, Peter Rehling, Blanche Schwappach, Ilaria Testa, Bolek Zapiec, Silvio O Rizzoli
Paraformaldehyde (PFA) is the most commonly used fixative for immunostaining of cells, but has been associated with various problems, ranging from loss of antigenicity to changes in morphology during fixation. We show here that the small dialdehyde glyoxal can successfully replace PFA Despite being less toxic than PFA, and, as most aldehydes, likely usable as a fixative, glyoxal has not yet been systematically tried in modern fluorescence microscopy. Here, we tested and optimized glyoxal fixation and surprisingly found it to be more efficient than PFA-based protocols...
November 16, 2017: EMBO Journal
https://www.readbyqxmd.com/read/29146509/isolated-coronal-shear-fracture-of-the-nonossified-humeral-trochlea-in-a-7-year-old-patient
#14
Michiyo Tomaru, Denju Osada, Kazuya Tamai, Hiroshi Taneichi
Coronal shear fractures of the nonossified humeral trochlea are extremely rare in skeletally immature children. This fracture is difficult to diagnose accurately on routine radiographs. In addition, the mechanism of injury and appropriate treatment methods are unknown. We present the case of a 7-year-old boy who sustained an isolated coronal shear fracture of the nonossified humeral trochlea. Internal fixation of the fracture was performed with 2 K-wires. Two and a half years after surgery, the patient had pain-free active range of motion of the elbow from 0° to 130° with full forearm rotation and magnetic resonance imaging showed healing of the chondral fragment of the trochlea...
November 14, 2017: Journal of Hand Surgery
https://www.readbyqxmd.com/read/29146433/a-novel-technique-for-cervical-facet-joint-hyperplasia-spondylotic-radiculopathy-by-laminar-and-lateral-mass-screw-co-fixations
#15
Sun-Ren Sheng, Ke Wang, Majid Nisar, Jiao-Xiang Chen, Ai-Min Wu, Xiang-Yang Wang
OBJECTIVE: To describe the novel technique and report the outcomes of cervical spondylotic radiculopathy caused by facet joint hyperplasia treated with minimally invasive surgery by laminar and lateral mass screw co-fixations. METHOD: In this retrospective study, patients with spondylotic radiculopathy caused by facet joint hyperplasia underwent this technique in our unit between January 2010 and June 2015. Hospital charts, magnetic resonance imaging studies, and follow-up records for all the patients were reviewed...
November 13, 2017: World Neurosurgery
https://www.readbyqxmd.com/read/29145978/outpatient-management-of-ankle-fractures
#16
REVIEW
Charles Qin, Robert G Dekker, Mia M Helfrich, Anish R Kadakia
Interest in outpatient orthopedic surgery has been fueled by provider desire to control costs and development of rapid recovery protocols. Open reduction and internal fixation (ORIF) is a commonly elected treatment strategy for ankle fracture that may be performed in an outpatient setting. Lessons on cost-savings of the outpatient model in orthopedics can be learned in total joint replacement and spine surgery. Moreover, in properly selected patients, outpatient ORIF has been shown to be comparably safe. Reasons for admission of the surgically managed patient with ankle fractures, including concern for surgical delay and additional social factors, warrant further investigation...
January 2018: Orthopedic Clinics of North America
https://www.readbyqxmd.com/read/29145968/effectiveness-of-a-step-by-step-oral-recount-before-a-practical-simulation-of-fracture-fixation
#17
Marcelo Abagge, Christiano Saliba Uliana, Sergei Taggesell Fischer, Kodi Edson Kojima
OBJECTIVE: To evaluate the effectiveness of a step-by-step oral recount by residents before the final execution of a practical exercise simulating a surgical fixation of a radial diaphyseal fracture. MATERIAL AND METHODS: The study included 10 residents of orthopaedics and traumatology (four second- year and six first-year residents) divided into two groups with five residents each. All participants initially gathered in a room in which a video was presented demonstrating the practical exercise to be performed...
October 2017: Injury
https://www.readbyqxmd.com/read/29145966/outcome-evaluation-of-staged-treatment-for-bicondylar-tibial-plateau-fractures
#18
Vincenzo Giordano, Ney Pecegueiro do Amaral, Hilton A Koch, Rodrigo Pires E Albuquerque, Felipe Serrão de Souza, José Félix Dos Santos Neto
BACKGROUND: The universal accepted strategy for treating high-energy tibial plateau fractures remains a topic of ongoing debate. The challenge for the practicing orthopaedic trauma surgeon is to provide anatomical articular fracture reduction, with successfully managing the complex soft-tissue injury that is commonly present at patient admission. The primary aim of the actual study was to evaluate the results of a staged protocol for the treatment of high-energy bicondylar tibial plateau fractures...
October 2017: Injury
https://www.readbyqxmd.com/read/29145963/functional-results-and-isokinetic-muscle-strength-in-patients-with-fraser-type-i-floating-knee-treated-with-internal-fixation
#19
Fernando Brandao Andrade-Silva, Adriana Carvalho, Caio Mansano, Aline Giese, Marcos de Camargo Leonhardt, Dennis Barbosa, Kodi Edson Kojima, Jorge Santos Silva
INTRODUCTION: According to Fraser's description, ipsilateral femoral and tibial diaphyseal fractures are characterised as type I floating knee and have a better prognosis than fractures with joint involvement (type II). There are few reports of functional and muscle strength in these patients. The objective of this study was to evaluate the functional and isokinetic muscle strength of patients with type I floating knee undergoing femoral and tibial internal fixation. METHODS: Patients with type I floating knee undergoing concomitant femoral and tibial internal fixation were invited for clinical evaluation...
October 2017: Injury
https://www.readbyqxmd.com/read/29145961/vertical-femoral-neck-fractures-in-young-adults-a-closed-fixation-strategy-using-a-transverse-cancellous-lag-screw
#20
João Antonio Matheus Guimarães, Leonardo Rosa Rocha, Tito Henrique Noronha Rocha, Danielle Cabral Bonfim, Rafaela Sartore da Costa, Amanda Dos Santos Cavalcanti, Carlos Rodrigo Roesler, Jamila Alessandra Perini Machado, Diego Pinheiro Aguiar, Maria Eugenia Leite Duarte
Vertical femoral neck fractures (Pauwels type III classification) in young adults generally occur as a consequence of high-energy trauma and are frequently seen in association with multiple injuries. Considering the controversies regarding the optimal fixation for this fracture, our aim was to evaluate the clinical outcome of a closed fixation strategy for vertical femoral neck fractures in young adults using two parallel and one transverse cancellous lag screws. This was a single-surgeon, prospective study including 20 young adults with average age of 38...
October 2017: Injury
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