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https://www.readbyqxmd.com/read/28930998/in-vitro-ovule-cultivation-for-live-cell-imaging-of-zygote-polarization-and-embryo-patterning-in-arabidopsis-thaliana
#1
Daisuke Kurihara, Yusuke Kimata, Tetsuya Higashiyama, Minako Ueda
In most flowering plants, the zygote and embryo are hidden deep in the mother tissue, and thus it has long been a mystery of how they develop dynamically; for example, how the zygote polarizes to establish the body axis and how the embryo specifies various cell fates during organ formation. This manuscript describes an in vitro ovule culture method to perform live-cell imaging of developing zygotes and embryos of Arabidopsis thaliana. The optimized cultivation medium allows zygotes or early embryos to grow into fertile plants...
September 11, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28930990/application-of-an-amplitude-integrated-eeg-monitor-cerebral-function-monitor-to-neonates
#2
Nora Bruns, Susanne Blumenthal, Irmgard Meyer, Susanne Klose-Verschuur, Ursula Felderhoff-Müser, Hanna Müller
Amplitude-integrated EEG (aEEG) is an easily accessible technique to monitor the electrocortical activity in preterm and term infants in neonatal intensive care units (NICUs). This method was first used to monitor newborns after asphyxia, providing information about future neurological outcomes. The aEEG is also helpful to select newborns who benefit from cooling. The aEEG monitoring of preterm infants is becoming more widespread, as various studies have shown that neurodevelopmental outcome is related to early aEEG tracings...
September 6, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28930926/fractures-of-the-nasal-bones-is-external-splinting-really-warranted
#3
Ourania Schoinohoriti, Dimosthenis Igoumenakis, George Rallis
BACKGROUND: It has been advocated that reduction of nasal bone fractures should be followed by internal packing and/or external splinting. Despite the ample literature concerning the advantages and limitations of various splint types, the necessity and effectiveness of external splinting has not been well documented. OBJECTIVE: To present the authors' experience and review the literature on treatment of nasal bone fractures, focusing on the indications and effectiveness of external splinting following closed reduction...
September 19, 2017: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/28930904/spontaneous-reduction-of-fractured-thoracolumbar-spine-with-complete-dislocation-case-report-with-literature-review
#4
Chen Zhao, Bing Zhang, Jiandang Shi, Yaping Li, Long Pang
Fractures and dislocations with complete lateral displacement of the spine at the lumbar and thoracic levels are some of the most severe types of thoracolumbar trauma. They are usually caused by high-energy trauma and accompanied by extreme, life-threatening injuries and neurological deficits that vary in severity, which may delay operative intervention. It is often difficult to reduce such a complex fracture-dislocation because of the force that the muscles exert on the bone and the injured part of the spinal cord...
September 2017: Orthopaedic Nursing
https://www.readbyqxmd.com/read/28930858/a-retrospective-study-comparing-percutaneous-and-open-pedicle-screw-fixation-for-thoracolumbar-fractures-with-spinal-injuries
#5
Bowen Wang, Yong Fan, Jingjing Dong, Hu Wang, Faqi Wang, Zhichen Liu, Haoyuan Liu, Yafei Feng, Fengrong Chen, Zheyuan Huang, Ruisong Chen, Wei Lei, Zixiang Wu
BACKGROUND: The purpose of this study was to evaluate the effectiveness between percutaneous and open pedicle screw fixation for treating thoracolumbar fractures with spinal injuries. METHODS: A total of 105 patients with thoracolumbar fractures and spinal injuries were divided into a percutaneous pedicle screw fixation (PPSF) group with 56 patients, who underwent percutaneous pedicle screw fixation, and an open pedicle screw fixation (OPSF) group with 49 patients, who underwent open pedicle screw fixation in accordance with the treatment project...
September 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28930856/the-role-of-ultrasonography-in-examination-of-the-stability-of-tile-b2-pelvic-fractures-7-case-reports-and-a-literature-review
#6
Bin-Fei Zhang, Hong Zhang, Peng-Fei Wang, Hu Wang, Jin-Lai Lei, Ya-Hui Fu, Yu-Xuan Cong, Hai Huang, Xiao-Ming Huo, Yan Zhuang, Kun Zhang
BACKGROUND: Determining whether a Tile-B2 pelvic fracture is stable is very challenging. We sought to identify the role of ultrasonography in determining the stability of Tile-B2 pelvic fractures. METHODS: We collected the clinical data of patients with Tile-B2 pelvic fractures who presented at Xi'an Hong-Hui Hospital between June 1, 2016, and August 5, 2016. The treatment strategy of each patient was determined by a team of senior surgeons in the department. A single sinologist observed the movement of the fracture sites in patients during rest, under compression, and during separation to determine fracture stability...
September 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28930778/radioprotection-with-amifostine-enhances-bone-strength-and-regeneration-and-bony-union-in-a-rat-model-of-mandibular-distraction-osteogenesis
#7
Catherine N Tchanque-Fossuo, Alexis Donneys, Sagar S Deshpande, Deniz Sarhaddi, Noah S Nelson, Laura A Monson, Sara E Dahle, Steve A Goldstein, Steven R Buchman
BACKGROUND: Using distraction osteogenesis (DO) to regenerate robust endogenous bone could greatly enhance postoncologic reconstruction of head and neck cancer. However, radiation (XRT) corrosive effects still preclude DO's immense potential. We posit that adjunctive pretreatment with the radioprotectant amifostine (AMF) can optimize wound healing and allow for successful DO with quantifiable enhancements in bony union and strength despite previous surgical bed irradiation. METHODS: Two groups of murine left hemimandibles were exposed to a human equivalent radiation dosage fractionated over 5 daily doses of 7 Gy...
September 19, 2017: Annals of Plastic Surgery
https://www.readbyqxmd.com/read/28930617/genetic-manipulation-of-microalgae-for-the-production-of-bioproducts
#8
Patricia Vazquez-Villegas, Mario A Torres-Acosta, Sergio A Garcia-Echauri, Jose M Aguilar-Yanez, Marco Rito-Palomares, Federico Ruiz-Ruiz
Microalgae have been used during the past four decades in the Bio-industries for the production of high added value products and development of useful approaches with environmental applications. The fast growing rate, simple growth requirements and using sunlight as the major source of energy are the key factors for usage of algae. In the past 15 years, a considerable progress has been made regarding the use of microalgae for production of proteins, nutraceuticals, food supplements, molecular tags for diagnostics and fixation of greenhouse gases...
January 1, 2018: Frontiers in Bioscience (Elite Edition)
https://www.readbyqxmd.com/read/28930518/microperimetric-biofeedback-training-fundamentals-strategies-and-perspectives
#9
Enzo Maria Vingolo, Giuseppe Napolitano, Serena Fragiotta
Microperimetric biofeedback training (MBFT) is a visual rehabilitative strategy based on fixation stability improvement reinforcing or creating a new preferential fixation locus. The rationale consists in reeducating visual system to a new visual condition, promoting retina-brain transmission, and thus cortical plasticity. The use of MBFT found is major application in visual diseases involving central vision, but later it revealed promising functional outcomes even in myopia, inherited retinal degenerations and nystagmus...
January 1, 2018: Frontiers in Bioscience (Scholar Edition)
https://www.readbyqxmd.com/read/28929828/increased-risk-of-aseptic-loosening-for-43-525-rotating-platform-vs-fixed-bearing-total-knee-replacements
#10
Oystein Gothesen, Stein Hakon L Lygre, Michelle Lorimer, Stephen Graves, Ove Furnes
Background and purpose - Given similar functional outcomes with mobile and fixed bearings, a difference in survivorship may favor either. This study investigated the risk of aseptic loosening for the most used subtypes of mobile-bearing rotating-platform knees, in Norway and Australia. Patients and methods - Primary TKRs reported to the Norwegian and Australian joint registries, between 2003 and 2014, were analyzed with aseptic loosening as primary end-point and all revisions as secondary end-point. We hypothesized that no difference would be found in the rate of revision between rotating-platform and the most used fixed-bearing TKRs, or between keeled and non-keeled tibia...
September 20, 2017: Acta Orthopaedica
https://www.readbyqxmd.com/read/28929800/microcosm-investigation-of-growth-and-phytoremediation-potential-of-azolla-japonica-along-nitrogen-gradients
#11
Hun Park, Uhram Song
Although Azolla species are among the most promising plants for use in phytoremediation, more studies on their growth and nitrogen (N) uptake along the N gradients of growing media are required. In this study, N concentration-dependent growth in growing media and phosphorus (P) and N accumulation by Azolla japonica were studied by estimating direct N uptake from media by molybdenum-iron proteins. The doubling time of A. japonica was less than a week, regardless of the N concentration (0, 5, and 25 mg N/L) present in the growth media, indicating that this plant is suitable for remediation...
October 3, 2017: International Journal of Phytoremediation
https://www.readbyqxmd.com/read/28929389/plate-fixation-of-the-proximal-humerus-an-international-multicentre-comparative-study-of-postoperative-complications
#12
Frank J P Beeres, N D L Hallensleben, S J Rhemrev, J C Goslings, F Oehme, S A G Meylaerts, R Babst, N W L Schep
OBJECTIVE: The primary aim was to evaluate the number of complications following locking plate fixation of proximal humeral fractures in country X and in country Y. The secondary aim was to identify risk factors for complications. METHODS: Multicentre retrospective case series of 282 consecutive patients with proximal humeral fractures, treated with a locking plate between 2010 and 2014. SETTING: two level 1 trauma centres in country X and one in country Y...
September 19, 2017: Archives of Orthopaedic and Trauma Surgery
https://www.readbyqxmd.com/read/28929365/management-of-comminuted-fractures-of-the-distal-humerus-clinical-outcome-after-primary-external-fixation-versus-immediate-fixation-with-locking-plates
#13
Clemens Kösters, Simon Lenschow, Esther Schulte-Zurhausen, Steffen Roßlenbroich, Michael J Raschke, Benedikt Schliemann
OBJECTIVES: The surgical treatment of comminuted fractures of the distal humerus remains a challenging problem. The aim of the present study was to compare the clinical outcomes of primary external fixation with second-staged open reduction and internal fixation (ORIF) and initial definitive internal fixation in surgically treated patients with comminuted distal humerus fractures. DESIGN: Retrospective comparative study. SETTING: Level one trauma center...
September 19, 2017: Archives of Orthopaedic and Trauma Surgery
https://www.readbyqxmd.com/read/28929195/patterns-of-bone-tracer-uptake-on-spect-ct-in-symptomatic-and-asymptomatic-patients-with-primary-total-hip-arthroplasty
#14
Thierry Schweizer, Filippo-Franco Schiapparelli, Niccolo Rotigliano, Helmut Rasch, Felix Amsler, Michael T Hirschmann
PURPOSE: The primary purpose of this study was to compare bone tracer uptake (BTU) on SPECT/CT in symptomatic and asymptomatic total hip arthroplasty (THA) and identify a possible relationship between BTU patterns and patient's symptoms. The secondary purpose was to investigate if the fixation methods (cemented versus uncemented) lead to different BTU patterns. METHODS: A total of 58 THAs, 31 symptomatic (group S) and 27 asymptomatic (group AS), were prospectively collected and retrospectively analyzed...
September 19, 2017: European Journal of Nuclear Medicine and Molecular Imaging
https://www.readbyqxmd.com/read/28928978/implantation-of-the-micra-leadless-pacemaker-in-a-patient-with-a-low-body-mass-index-of-16
#15
Gary Tse, Tong Liu, Guangping Li, Wing Tak Wong, Gary Chin Pang Chan, Yat Sun Chan, Bryan Yan
A 71-year-old female patient has a history of complete heart block and recurrent pacemaker site infection requiring multiple pacemaker explanations. A leadless pacemaker using passive fixation was inserted into the right ventricular apex via transvenous approach without complications. This case illustrates the feasibility of implanting a leadless pacemaker system in a small-sized adult with a low body mass index of 16 (weight: 33.7 kg, height: 145 cm) which may have potential application in elderly Asian subjects...
September 2017: Oxford Medical Case Reports
https://www.readbyqxmd.com/read/28928953/treatment-of-femoral-shaft-fractures-with-monoaxial-external-fixation-in-polytrauma-patients
#16
Gianluca Testa, Domenico Aloj, Alessandro Ghirri, Eraclite Petruccelli, Vito Pavone, Alessandro Massé
Background: Femoral shaft fractures, typical in younger people, are often associated with polytrauma followed by traumatic shock. In these situations, despite intramedullary nailing being the treatment of choice, external fixation could be used as the definitive treatment. The aim of this study is to report evidence regarding definitive treatment of femoral shaft fractures with monoaxial external fixation. Methods: Between January 2006 and December 2015, 83 patients with 87 fractures were treated at the Department of Orthopaedics and Traumatology CTO of Turin, with a monoaxial external fixation device...
2017: F1000Research
https://www.readbyqxmd.com/read/28928818/the-value-of-computer-assisted-navigation-for-bone-reconstruction-after-tumor-resection
#17
Xuejun Peng, Fengping Wang, Jing Su
This study was designed to evaluate the use of computer-assisted navigation with computed tomography (CT) images for bone reconstruction after resection in malignant bone tumor treatment. Forty-five patients with malignant bone tumors were recruited for this study. CT scan images in a computer-assisted navigation system were used to assist during the osteotomy, the pairing with allografts, and the monitoring of the allograft and joint lines to perform joint reconstruction. Our results show that osteotomy and allograft pairing were successful in all patients...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28928719/the-green-berry-consortia-of-the-sippewissett-salt-marsh-millimeter-sized-aggregates-of-diazotrophic-unicellular-cyanobacteria
#18
Elizabeth G Wilbanks, Verena Salman-Carvalho, Ulrike Jaekel, Parris T Humphrey, Jonathan A Eisen, Daniel H Buckley, Stephen H Zinder
Microbial interactions driving key biogeochemical fluxes often occur within multispecies consortia that form spatially heterogeneous microenvironments. Here, we describe the "green berry" consortia of the Sippewissett salt marsh (Falmouth, MA, United States): millimeter-sized aggregates dominated by an uncultured, diazotrophic unicellular cyanobacterium of the order Chroococcales (termed GB-CYN1). We show that GB-CYN1 is closely related to Crocosphaera watsonii (UCYN-B) and "Candidatus Atelocyanobacterium thalassa" (UCYN-A), two groups of unicellular diazotrophic cyanobacteria that play an important role in marine primary production...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28928010/three-dimensional-strut-plate-for-the-treatment-of-mandibular-fractures-a-systematic-review
#19
REVIEW
J C S de Oliveira, L B Moura, J D S de Menezes, M A C Gabrielli, V A Pereira Filho, E Hochuli-Vieira
The treatment of mandibular fractures by open reduction and internal fixation is very variable. Thus, there are many controversies about the best fixation system in terms of stability, functional recovery, and postoperative complications. This systematic review sought scientific evidence regarding the best indication for the use of three-dimensional (3D) plates in the treatment of mandibular fractures. A systematic search of the PubMed/MEDLINE, Elsevier/Scopus, and Cochrane Library databases was conducted to include articles published up until November 2016...
September 16, 2017: International Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/28927955/modified-hybrid-fixation-using-absorbable-plate-and-screw-for-mandibular-advancement-surgery
#20
Koichiro Ueki, Kunio Yoshizawa, Akinori Moroi, Asami Hotta, Takamitsu Tsutsui, Kenichi Fukaya, Ryota Hiraide, Akihiro Takayama, Tatsuya Tsunoda, Yuki Saito, Nana Baba, Momoko Sato
PURPOSE: The purpose of this study was to examine the skeletal stability of mandibular advancement after sagittal split ramus osteotomy (SSRO) with modified hybrid fixation using absorbable plates and screws. MATERIALS AND METHODS: A total of 54 Japanese patients were enrolled in this study. Of them, 23 who were diagnosed with mandibular prognathism underwent setback surgery with bi-cortical plate fixation using absorbable plates and screws (setback group). Another 23 who were diagnosed with mandibular retrognathism underwent advancement surgery with modified hybrid fixation using absorbable plates (advance group), and 8 who were diagnosed with mandibular retrognathism underwent advancement surgery with titanium plates (titanium group)...
August 10, 2017: Journal of Cranio-maxillo-facial Surgery
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