Read by QxMD icon Read

Glutathione peroxidase

Luís M Félix, Ana M Vidal, Cindy Serafim, Ana M Valentim, Luís M Antunes, Sandra M Monteiro, Manuela Matos, Ana M Coimbra
Ketamine is a widely used pharmaceutical that has been detected in water sources worldwide. Zebrafish embryos were used in this study to investigate the oxidative stress and apoptotic signals following a 24h exposure to different ketamine concentrations (0, 50, 70 and 90 mg L-1 ). Early blastula embryos (∼2 h post fertilisation-hpf) were exposed for 24 h and analysed at 8 and 26 hpf. Reactive oxygen species and apoptotic cells were identified in vivo, at 26 hpf. Enzymatic activities (superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), lactate dehydrogenase (LDH) and acetylcholinesterase (AChE)), glutathione levels (oxidised (GSSG) and reduced (GSH)), oxidative damage (lipid peroxidation (LPO) and protein carbonyls (CO)) as well as oxidative stress (gclc, gstp1, sod1 and cat), apoptosis (casp3a, casp6, casp8, casp9, aifm1 and tp53) and cell proliferation (pcna) related-genes were evaluated at 8 and 26 hpf...
March 7, 2018: Chemosphere
Donatella Pietraforte, Eleonora Paulicelli, Clarice Patrono, Lucrezia Gambardella, Giuseppe Scorza, Antonella Testa, Paola Fattibene
Reactive oxygen species (ROS) are important mediators of the cytotoxicity induced by the direct reaction of ionising radiation (IR) with all critical cellular components, such as proteins, lipids, and nucleic acids. The derived oxidative damage may propagate in exposed tissues in a dose- and spatiotemporal dependent manner to other cell compartments, affecting intracellular signalling, and cell fate. To understand how cell damage is induced, we studied the oxidative events occurring immediately after cell irradiation by analysing the fate of IR-derived ROS, the intracellular oxidative damage, and the modification of redox environment accumulating in Chinese hamster ovary (CHO) within 1 h after cell irradiation (dose range 0-10 Gy)...
March 16, 2018: Free Radical Research
S J Hartman, O N Genther-Schroeder, S L Hansen
To compare trace mineral (TM) repletion in feedlot steers after depletion by S and Mo, 72 Red Angus steers blocked by BW (253 ± 14 kg) were assigned (6 steers per pen, fed via GrowSafe bunks) to corn silage depletion diets (DEP) supplemented with NRC (1996) recommended concentrations of Cu, Mn, Se, and Zn (CON) or supplemented with 0.3% S (CaSO4), 2 mg of Mo/kg DM, and no added Cu, Mn, Zn, or Se (ANT). Three 62 d TM repletion strategies (REP) were applied within DEP diets on d 89: 1) Multimin90 injection (contains Cu, Mn, Se, Zn) and 100% of recommended Cu, Mn, Zn, and Se from inorganic sources (ITM), 2) saline injection and 150% of recommended TM from inorganic sources (ING), or 3) saline injection and 150% of recommended TM provided as 25% organic and 75% inorganic sources (BLEND)...
March 13, 2018: Journal of Animal Science
Milad Moloudizargari, Esmaeil Mortaz, Mohammad Hossein Asghari, Ian M Adcock, Frank A Redegeld, Johan Garssen
Omega-3 polyunsaturated fatty acids (PUFAs) have well established anti-cancer properties. Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are among this biologically active family of macromolecules for which various anti-cancer effects have been explained. These PUFAs have a high safety profile and can induce apoptosis and inhibit growth of cancer cells both in vitro and in vivo , following a partially selective manner. They also increase the efficacy of chemotherapeutic agents by increasing the sensitivity of different cell lines to specific anti-neoplastic drugs...
February 20, 2018: Oncotarget
Raúl Cassia, Macarena Nocioni, Natalia Correa-Aragunde, Lorenzo Lamattina
Here, we review information on how plants face redox imbalance caused by climate change, and focus on the role of nitric oxide (NO) in this response. Life on Earth is possible thanks to greenhouse effect. Without it, temperature on Earth's surface would be around -19°C, instead of the current average of 14°C. Greenhouse effect is produced by greenhouse gasses (GHG) like water vapor, carbon dioxide (CO2 ), methane (CH4 ), nitrous oxides (Nx O) and ozone (O3 ). GHG have natural and anthropogenic origin. However, increasing GHG provokes extreme climate changes such as floods, droughts and heat, which induce reactive oxygen species (ROS) and oxidative stress in plants...
2018: Frontiers in Plant Science
Noura Brahmi, Mongi Saoudi, Yamina Kadri, Chomous Kallel, Aida El Euch, Fatma Makni Ayadi, Abdel Halim Harrath, Abdelfatah El Feki, Mohamed Salah Allagui
High fat diet (HFD) exposure is associated with various pathological dysfunctions, including haematological disorders and oxidative stress. The in vitro analysis of AECG revealed the presence of important levels of polyphenols and flavonoids, and denoted antioxidant capacities confirmed by nitric oxide radical (NO• ), reducing the power and HPLC chemical components' determinations. The animals exposed to HFD revealed a severe damage in the blood cells structure and haematological parameters accompanied with a significant decrease in serum Mg2+ and Ca2+ ATPase activities...
March 15, 2018: Archives of Physiology and Biochemistry
Stefano Magni, François Gagné, Chantale André, Camilla Della Torre, Joëlle Auclair, Houda Hanana, Camilla Carla Parenti, Francesco Bonasoro, Andrea Binelli
Microplastics (MPs), plastic debris smaller than 5mm, are widely found in both marine and freshwater ecosystems. However, few studies regarding their hazardous effects on inland water organisms, have been conducted. For this reason, the aim of our research was the evaluation of uptake and chronic toxicity of two mixtures (MIXs) of virgin polystyrene microbeads (PMs) of 10μm and 1μm in size (MIX 1, with 5×105 of 1μmsizePMs/L and 5×105 of 10μmsizePMs/L, and MIX 2 with 2×106 of 1μmsizePMs/L and 2×106 of 10μmsizePMs/L) on freshwater zebra mussel Dreissena polymorpha (Mollusca: Bivalvia) during 6 exposure days...
March 12, 2018: Science of the Total Environment
Magdalena B Król, Michał Galicki, Peter Grešner, Edyta Wieczorek, Ewa Jabłońska, Edyta Reszka, Zbigniew Morawiec, Wojciech Wąsowicz, Jolanta Gromadzińska
BACKGROUND: The aim of this study was to establish whether the gene expression of estrogen receptor alpha (encoded by ESR1) correlates with the expression of glutathione peroxidase 1 (encoded by GPX1) in the tumor and adjacent tumor-free breast tissue, and whether this correlation is affected by breast cancer. Such relationships may give further insights into breast cancer pathology with respect to the status of estrogen receptor. METHODS: We used the quantitative real-time PCR technique to analyze differences in the expression levels of the ESR1 and GPX1 genes in paired malignant and non-malignant tissues from breast cancer patients...
March 15, 2018: Acta Biochimica Polonica
Phaniendra Alugoju, Vkd Krishan Swamy, Latha Periyasamy
Aging is characterized by gradual accumulation of macromolecular damage leading to progressive loss of physiological function and increased susceptibility to diverse diseases. Effective anti-aging strategies involving caloric restriction or antioxidant supplementation are receiving growing attention to attenuate macromolecular damage in age associated pathology. In the present study, we for the first time investigated the effect of quercetin, caloric restriction and combined treatment (caloric restriction with quercetin) on oxidative stress parameters, acetylcholinesterase and ATPases enzyme activities in the cerebral cortex of aged male wistar rat brain...
March 14, 2018: CNS & Neurological Disorders Drug Targets
Thomas George Back, Kai N Sands, Tyler A Tuck
Selenium compounds play an important role in redox homeostasis in living organisms. One of their major functions is to suppress the harmful effects of hydrogen peroxide, hydroperoxides and downstream reactive oxygen species that lead to oxidative stress, which has in turn been implicated in many diseases and degenerative conditions. The glutathione peroxidase (GPx) family of selenoenzymes plays a key protective role by catalyzing the reduction of peroxides with glutathione. Considerable effort has been expended toward the discovery of small-molecule selenium compounds that mimic GPx...
March 14, 2018: Chemistry: a European Journal
Chen Chen, Wenzhong Hu, Ruidong Zhang, Aili Jiang, Chenghui Liu
BACKGROUND: Effect of hydrogen sulfide (H2 S) on inhibiting surface whitening of fresh-cut carrots was investigated. Whitening index, H2 O2 and malondialdehyde (MDA) contents, the activities of catalase (CAT), ascorbate peroxidase (APX), glutathione reductase (GR), phenylalanine ammonialyase (PAL), polyphenol oxidase (PPO) and peroxidase (POD) and antioxidant capacities were evaluated. RESULTS: H2 S treatment significantly inhibited the surface whitening of fresh-cut carrots (P < 0...
March 14, 2018: Journal of the Science of Food and Agriculture
Xiaofeng Wang, Wei Wang, Wei Li, Zhiduo Niu, Qing Wang, Dianwen Zhang, Hui Li, Jincheng Wang
Local drug delivery systems have been proposed for the treatment of periodontitis, and Hylotelephium purpureum may be a suitable agent for use in such a system. The present study aimed to formulate a Hylotelephium purpureum gel (HPG) and evaluate the anti-inflammatory activity and antinociceptive effects of Hylotelephium purpureum and to assess the duration of action and efficacy of HPG in the treatment of experimental periodontitis in a KM mouse model. First, an acute toxicity study was performed, and secondly, xylene-induced mouse-ear edema, acetic acid-induced mouse peritoneal capillary permeability and carrageenan-induced hind-paw edema tests were used to investigate the anti-inflammatory activity of the gel...
April 2018: Biomedical Reports
Zhaoheng Liu, Wei Wang, Fang Cao, Shuo Liu, Xinxin Zou, Guodong Li, Haojie Yang, Yang Jiao
Air pollution is the main cause of respiratory diseases. Fine particulates with the diameter below 2.5  μ m can get into the alveoli and then enter the blood circulation through the lung tissue ventilation function and cause multiple systemic diseases especially the respiratory diseases. This study investigated the pathological mechanism of the lungs injury in rats induced by PM2.5 and the effect and mechanism of the Chinese herbal medicine number 2 Feibi Recipe (number 2 FBR) on lungs injury. In this experiment, Wistar rats were used...
2018: Evidence-based Complementary and Alternative Medicine: ECAM
Min Yang, Youcong Chuan, Cunwu Guo, Jingjing Liao, Yanguo Xu, Xinyue Mei, Yixiang Liu, Huichuan Huang, Xiahong He, Shusheng Zhu
Panax notoginseng is a highly valuable medicinal herb, but its culture is strongly hindered by replant failure, mainly due to autotoxicity. Deciphering the response mechanisms of plants to autotoxins is critical for overcoming the observed autotoxicity. Here, we elucidated the response of P. notoginseng to the autotoxic ginsenoside Rg1 via transcriptomic and cellular approaches. Cellular analyses demonstrated that Rg1 inhibited root growth by disrupting the cell membrane and wall. Transcriptomic analyses confirmed that genes related to the cell membrane, cell wall decomposition and reactive oxygen species (ROS) metabolism were up-regulated by Rg1 stress...
2018: Frontiers in Plant Science
Cuimei Zhang, Shangli Shi
Drought severely limits global plant distribution and agricultural production. Elucidating the physiological and molecular mechanisms governing alfalfa stress responses will contribute to the improvement of drought tolerance in leguminous crops. In this study, the physiological and proteomic responses of two alfalfa ( Medicago sativa L.) varieties contrasting in drought tolerance, Longzhong (drought-tolerant) and Gannong No. 3 (drought-sensitive), were comparatively assayed when seedlings were exposed to -1...
2018: Frontiers in Plant Science
Guglielmo Duranti, Roberta Ceci, Federica Patrizio, Paolo Sgrò, Luigi Di Luigi, Stefania Sabatini, Francesco Felici, Ilenia Bazzucchi
The polyphenolic flavonoid quercetin has been shown to be a powerful antioxidant, in vitro and in murine models. However, its effect on redox status has been poorly examined in humans, particularly in combination with strenuous exercise. We hypothesized that quercetin supplementation would beneficially affect redox homeostasis in healthy individuals undergoing eccentric exercise. To test this hypothesis, the effects of chronic consumption of quercetin on glutathione system (reduced, oxidized, and reduced to oxidized glutathione ratio), oxidative damage [thiobarbituric acid reactive substances (TBARs)], antioxidant enzymatic network (catalase, glutathione peroxidase, superoxide dismutase) and resistance to lysis, were investigated in erythrocytes, a traditional model widely used to study the effects of oxidative stress as well as the protective effects of antioxidants...
February 2018: Nutrition Research
Xiaopeng Tang, Bo Liu, Xiangrong Wang, Qifang Yu, Rejun Fang
The epidermal growth factor (EGF) has been widely used for protection of stress-induced intestinal mucosa dysfunction. However, whether EGF would alleviate oxidative injury and reduce apoptosis in porcine intestine is not yet known. Therefore, the aim of this study was to investigate the effect of EGF on lipopolysaccharides (LPS)-induced induction of oxidative stress and ensuing apoptosis in the porcine intestinal epithelial cell line, IPEC-J2. The present study showed that EGF significantly increased cell viability and decreased the LPS-induced induction of apoptosis, dehydrogenase (LDH) release and malonaldehyde (MDA) production...
March 14, 2018: International Journal of Molecular Sciences
Pasquale Pignatelli, Danilo Menichelli, Daniele Pastori, Francesco Violi
The role of oxidative stress in the onset and progression of atherosclerosis and its impact on the development of cardiovascular events has been widely described. Thus, an increased oxidative stress has been described in several atherosclerotic risk factors, such as hypertension, dyslipidaemia, peripheral artery disease, metabolic syndrome, diabetes and obesity. Among others, specific oxidative pathways involving both pro-oxidant and antioxidant enzymes seem to play a major role in the production of reactive oxidant species (ROS), such as NADPH oxidase, myeloperoxidase, superoxide dismutase, glutathione peroxidase...
March 14, 2018: Kardiologia Polska
M Yasar, I Erdi, B Kaya
AIM: We investigated the effects of atorvastatin and N-acetyl cysteine in decreasing ischemia-reperfusion damage after detorsion of a volvulus of the cecum and ascending colon. METHODS: Wistar albino rats (250-300 g) were divided into four groups. A cecal-ascending colon volvulus was created by the intestinal clockwise 720° rotation. At the end of one hour, the bowel was detorsioned. Group I (n = 7) was the sham (laparotomy) group, Group II (n = 7) the control (no treatment, volvulus or detorsion), Group III (n = 7) (N-acetyl cysteine administered ) , and Group IV (n = 7) (atorvastatin administered ) group...
2018: Bratislavské Lekárske Listy
K A Sudharshana Murthy, Ambarisha Bhandiwada, Shivani L Chandan, Surakshith L Gowda, G Sindhusree
Background: Prevalence of Gestational Diabetes Mellitus in India is increasing. In addition to performing physiological role in fetoplacental unit during pregnancy, cytokines also play pathophysiological role if expressed in abnormal amounts or sites. Objective: To estimate Proinflammatory Cytokines TNF-α, IL-6, IL-8 and anti-oxidants Glutathione Peroxidase (GTX), Superoxide dismutase (SOD), uric acid and Bilirubin levels in GDM and correlate with pregnancy outcome...
January 2018: Indian Journal of Endocrinology and Metabolism
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"