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https://www.readbyqxmd.com/read/28330253/parameters-influencing-agrobacterium-mediated-transformation-system-in-safflower-genotypes-aks-207-and-pkv-pink
#1
Dipti Raghunath Dhumale, Prashant Raghunath Shingote, Mahendra Shankarrao Dudhare, Pravin Vishwanath Jadhav, Prashant Bhaskar Kale
Shoot regeneration in safflower (Carthamus tinctorius 'AKS 207' and 'PKV Pink') genetically transformed using Agrobacterium was used for assessing various constraints to the efficiency of transformation including infection period, virulence induction medium, co-cultivation period, bacterial titre, selection regime, and the natural phenolic compound acetosyringone. Transformation frequency was promising with 8-10-day-old cotyledonary leaf explants. Therefore, explants of that age cultured on Agrobacterium minimal medium (AB) containing 100 µM acetosyringone were infected with Agrobacterium (cell titre 0...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28329800/characterizing-readiness-for-advance-care-planning-from-the-perspective-of-residents-families-and-clinicians-an-interpretive-descriptive-study-in-supportive-living
#2
Marta Shaw, Jennifer Hewson, David B Hogan, Shelley Raffin Bouchal, Jessica Simon
Background and Objectives: Advance care planning (ACP) involves reflection on health care preferences and communication of the person's wishes in case of future incapacity. ACP is particularly pertinent in supportive living (SL) as residents are at high risk of cognitive decline and changes in health status, but the readiness of residents, families, and health care practitioners to engage in ACP discussions is not known. The purpose of this study was to explore the readiness among these stakeholders and the factors that influence the degree of readiness...
February 18, 2017: Gerontologist
https://www.readbyqxmd.com/read/28328497/synergistic-effects-of-nanodrug-ultrasound-hyperthermia-and-thermal-ablation-on-solid-tumors-an-animal-study
#3
Li-Chen Chiu, Sheng-Kai Wu, Win-Li Lin, Gin-Shin Chen
OBJECTIVE: Delivery barriers of nanodrug in large tumors due to heterogeneous blood supply, elevated interstitial pressure and long transport distances can degrade the efficacy of cancer treatment. In this study, we proposed a therapeutic strategy to improve the tumor growth inhibition by injecting pegylated liposomal doxorubicin (PLD), and then applying a short time of ultrasound hyperthermia (HT) on the entire solid tumor, and inflicting ultrasound thermal ablation (Ab) in the low-perfused tumor region...
March 17, 2017: IEEE Transactions on Bio-medical Engineering
https://www.readbyqxmd.com/read/28327967/genome-sequence-of-pacific-abalone-haliotis-discus-hannai-the-first-draft-genome-in-family-haliotidae
#4
Bo-Hye Nam, Woori Kwak, Young-Ok Kim, Dong-Gyun Kim, Hee Jeong Kong, Woo-Jin Kim, Jeong-Ha Kang, Jung Youn Park, Cheul Min An, Ji-Young Moon, Choul Ji Park, Jae Woong Yu, Joon Yoon, Minseok Seo, Kwondo Kim, Duk Kyung Kim, SaetByeol Lee, Samsun Sung, Chul Lee, Younhee Shin, Myunghee Jung, Byeong-Chul Kang, Shin Ga-Hee, Sojeong Ka, Kelsey Caetano-Anolles, Seoae Cho, Heebal Kim
Background: Abalones are large marine snails in the family Haliotidae and the genus Haliotis belonging to the class Gastropoda of the phylum Mollusca. The family Haliotidae contains only one genus, Haliotis, and this single genus is known to contain several species of abalone. With 18 additional subspecies, the most comprehensive treatment of Haliotidae considers 56 species valid [1]. Abalone is an economically important fishery and aquaculture animal which is considered a highly-prized seafood delicacy...
March 7, 2017: GigaScience
https://www.readbyqxmd.com/read/28327552/probing-medin-monomer-structure-and-its-amyloid-nucleation-using-13-c-direct-detection-nmr-in-combination-with-structural-bioinformatics
#5
Hannah A Davies, Daniel J Rigden, Marie M Phelan, Jillian Madine
Aortic medial amyloid is the most prevalent amyloid found to date, but remarkably little is known about it. It is characterised by aberrant deposition of a 5.4 kDa protein called medin within the medial layer of large arteries. Here we employ a combined approach of ab initio protein modelling and (13)C-direct detection NMR to generate a model for soluble monomeric medin comprising a stable core of three β-strands and shorter more labile strands at the termini. Molecular dynamics simulations suggested that detachment of the short, C-terminal β-strand from the soluble fold exposes key amyloidogenic regions as a potential site of nucleation enabling dimerisation and subsequent fibril formation...
March 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28327129/short-term-overfeeding-of-zebrafish-with-normal-or-high-fat-diet-as-a-model-for-the-development-of-metabolically-healthy-versus-unhealthy-obesity
#6
Kathrin Landgraf, Susanne Schuster, Andrej Meusel, Antje Garten, Thomas Riemer, Dorit Schleinitz, Wieland Kiess, Antje Körner
BACKGROUND: Obese individuals differ in their risk of developing metabolic and cardiovascular complications depending on fat distribution (subcutaneous versus visceral) and adipose tissue (AT) phenotype (hyperplasic versus hypertrophic). However, the exact mechanisms which determine whether an obese individual is metabolically healthy or unhealthy are not clear, and analyses of the underlying pathomechanisms are limited by the lack of suitable in vivo models in which metabolically healthy versus metabolically unhealthy AT accumulation can be specifically induced...
March 21, 2017: BMC Physiology
https://www.readbyqxmd.com/read/28325875/ab-initio-solution-of-macromolecular-crystal-structures-without-direct-methods
#7
Airlie J McCoy, Robert D Oeffner, Antoni G Wrobel, Juha R M Ojala, Karl Tryggvason, Bernhard Lohkamp, Randy J Read
The majority of macromolecular crystal structures are determined using the method of molecular replacement, in which known related structures are rotated and translated to provide an initial atomic model for the new structure. A theoretical understanding of the signal-to-noise ratio in likelihood-based molecular replacement searches has been developed to account for the influence of model quality and completeness, as well as the resolution of the diffraction data. Here we show that, contrary to current belief, molecular replacement need not be restricted to the use of models comprising a substantial fraction of the unknown structure...
March 21, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28325316/removal-of-dye-by-carboxymethyl-cellulose-acrylamide-and-graphene-oxide-via-a-free-radical-polymerization-process
#8
Kokkarachedu Varaprasad, Tippabattini Jayaramudu, Emmanuel Rotimi Sadiku
Carboxymethyl cellulose has been used for the design of novel engineered hydrogels in order to obtain effective three-dimensional structures for industrial applications. In this work, dye removal carboxymethyl cellulose-acrylamide-graphene oxide (CMC-AM-GO) hydrogels were prepared by a free-radical polymerization method. The GO was developed by the modified Hummers method. The CMC-AM-GO and GO were characterized by FTIR, XRD and SEM. The swelling and swelling kinetics were calculated using gravimetric process...
May 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28324832/determination-of-the-interconversion-energy-barrier-of-three-novel-pentahelicene-derivative-enantiomers-by-dynamic-high-resolution-liquid-chromatography
#9
Pavel Majek, Jan Krupcik, Zachary S Breitbach, Milan K Dissanayake, Peter Kroll, Aaron A Ruch, LeGrande M Slaughter, Daniel W Armstrong
Dynamic high resolution liquid chromatography (DHPLC) was used to determine the kinetic and thermodynamic activation parameters of interconversion of three novel pentahelicene derivatives {3,5-bis(trifluoromethyl)benzo[i]pentahelicene, naphtho[1,2-i]pentahelicene and 4-methoxybenzo[i]pentahelicene}. DHPLC was performed on a chiral isopropyl - carbamate cyclofructan 6 (LARIHC CF6-P) column under normal phase conditions. Variation of the column temperature and flow rate was used to study the interconversion process...
December 21, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28324142/a-phase-ii-study-of-partial-and-subtotal-thymectomy-for-thymoma-jart02
#10
Motoki Yano, Yoshitaka Fujii, Junji Yoshida, Tomoki Utsumi, Hiroyuki Shiono, Motoshi Takao, Masayuki Tanahashi, Yushi Saito
BACKGROUND: We believe the merit of preservation of a part of the thymus following surgery for thymoma. We evaluated the efficacy of partial or subtotal thymectomy for early-stage thymoma in the prospective study. METHODS: The Japanese Association for Research on the Thymus conducted a multiple institutional study of thymectomy for thymoma localized in the thymus without total thymectomy. Patients without autoimmune disease who had an anterior mediastinal tumor that had been clinically diagnosed as an early-stage thymoma were enrolled in the study...
March 21, 2017: World Journal of Surgery
https://www.readbyqxmd.com/read/28323386/the-association-of-maternal-lymphatic-markers-and-critical-congenital-heart-defects-in-the-fetus-a-population-based-case-control-study
#11
Martina A Steurer, Mary E Norton, Rebecca J Baer, Gary M Shaw, Sheila Keating, Anita J Moon-Grady, Christina D Chambers, Laura L Jelliffe-Pawlowski
The objective ot this study was to investigate whether lymphatic markers measured in women during the second trimester are associated with critical congenital heart defects (CCHDs) in offspring. This is a retrospective cohort study of pregnant women who participated in the California Prenatal Screening Program. CCHD data in the offspring was captured by linking birth certificate data with hospital patient discharge records. Second trimester samples were assayed for vascular endothelial growth factor (VEGF), platelet derived growth factor (PDGF) AA/BB, and PDGF AB...
March 21, 2017: American Journal of Medical Genetics. Part A
https://www.readbyqxmd.com/read/28323071/the-central-role-of-trna-in-genetic-code-expansion
#12
REVIEW
Noah M Reynolds, Oscar Vargas-Rodriguez, Dieter Söll, Ana Crnković
BACKGROUND: The development of orthogonal translation systems (OTSs) for genetic code expansion (GCE) has allowed for the incorporation of a diverse array of non-canonical amino acids (ncAA) into proteins. Transfer RNA, the central molecule in the translation of the genetic message into proteins, plays a significant role in the efficiency of ncAA incorporation. SCOPE OF REVIEW: Here we review the biochemical basis of OTSs for genetic code expansion. We focus on the role of tRNA and discuss strategies used to engineer tRNA for the improvement of ncAA incorporation into proteins...
March 17, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28322842/simultaneous-blockade-of-nmda-receptors-and-parp-1-activity-synergistically-alleviate-immunoexcitotoxicity-and-bioenergetics-in-3-nitropropionic-acid-intoxicated-mice-evidences-from-memantine-and-3-aminobenzamide-interventions
#13
Saravana Babu Chidambaram, Ranju Vijayan, Sathiya Sekar, Sugumar Mani, Barathidsan Rajamani, Ramakrishnan Ganapathy
Interlink between excitotoxicity and cellular bioenergetics depletion is implicated as one of the central deteriorative pathways in many neurodegenerative diseases including Huntington's disease (HD). Chronic administration of 3-nitropropionic acid (3-NP) depletes ATP and NAD(+;) and increases TNFα, IL-6 and glutamate content resulting in "immunoexcitotoxicity". Present study was designed to determine whether the combination of memantine (MN) and 3-aminobenzamide (3-AB), PARP inhibitor, can ameliorate immunoexcitotoxicity and improve bioenergetics in a better manner than individual administration against 3-NP intoxication in mice...
March 16, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28322554/schottky-barriers-in-bilayer-phosphorene-transistors
#14
Yuanyuan Pan, Yang Dan, Yangyang Wang, Meng Ye, Han Zhang, Ruge Quhe, Xiuying Zhang, Jingzhen Li, Wanlin Guo, Li Yang, Jing Lu
It is unreliable to evaluate the Schottky barrier height (SBH) in monolayer (ML) 2D material field effect transistors (FETs) with strongly interacted electrode from the work function approximation (WFA) because of existence of the Fermi-level pinning. Here, we report the first systematical study of bilayer (BL) phosphorene FETs in contact with a series of metals with a wide work function range ( Al, Ag, Cu, Au, Cr, Ti, Ni, and Pd) by using both ab initio electronic band calculations and quantum transport simulation (QTS)...
March 21, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28322513/site-of-metabolism-prediction-based-on-ab-initio-derived-atom-representations
#15
Arndt R Finkelmann, Andreas H Göller, Gisbert Schneider
Machine learning models for site of metabolism (SoM) prediction offer the ability to identify metabolic soft spots in low molecular weight drug molecules at low computational cost and enable data-based reactivity prediction. SoM prediction is an atom classification problem. Successful construction of machine learning models requires atom representations that capture the reactivity-determining features of a potential reaction site. We have developed a descriptor scheme that characterizes an atom's steric and electronic environment and its relative location in the molecular structure...
March 21, 2017: ChemMedChem
https://www.readbyqxmd.com/read/28322383/unique-agreement-of-experimental-and-computational-infrared-spectroscopy-a-case-study-of-lithium-bromide-solvation-in-an-important-electrochemical-solvent
#16
Maciej Smiechowski, Joanna Krakowiak, Piotr Bruździak, Janusz Stangret
Infrared (IR) spectroscopy is a widely used and invaluable tool in the studies of solvation phenomena in electrolyte solutions. Using state-of-the-art chemometric analysis of a spectral series measured in a concentration-dependent manner, the spectrum of the solute-affected solvent can be extracted, providing a detailed view of the structural and energetic states of the solvent molecules influenced by the solute. Concurrently, ab initio molecular dynamics (AIMD) simulations provide the solvation shell picture at an atomistic detail level and allow for a consistent decomposition of the theoretical IR spectrum in terms of distance-dependent contributions of the solvent molecules...
March 21, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28322343/unprecedented-simultaneous-enhancement-in-damage-tolerance-and-fatigue-resistance-of-zirconia-ta-composites
#17
A Smirnov, J I Beltrán, T Rodriguez-Suarez, C Pecharromán, M C Muñoz, J S Moya, J F Bartolomé
Dense (>98 th%) and homogeneous ceramic/metal composites were obtained by spark plasma sintering (SPS) using ZrO2 and lamellar metallic powders of tantalum or niobium (20 vol.%) as starting materials. The present study has demonstrated the unique and unpredicted simultaneous enhancement in toughness and strength with very high flaw tolerance of zirconia/Ta composites. In addition to their excellent static mechanical properties, these composites also have exceptional resistance to fatigue loading. It has been shown that the major contributions to toughening are the resulting crack bridging and plastic deformation of the metallic particles, together with crack deflection and interfacial debonding, which is compatible with the coexistence in the composite of both, strong and weak ceramic/metal interfaces, in agreement with predictions of ab-initio calculations...
March 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28322115/lower-extremity-kinematics-of-curve-sprinting-displayed-by-runners-using-a-transtibial-prosthesis
#18
Yumeng Li, Kathy J Simpson, Lee Nolan, Marilyn Miller, Benjamin Johnson
The purpose of the study was to determine if the kinematics exhibited by skilled runners wearing a unilateral, transtibial prosthesis during the curve section of a 200-m sprint race were influenced by interaction of limb-type (prosthetic limb (PROS-L) vs. nonprosthetic limb (NONPROS-L)) and curve-side (inside and outside limb relative to the centre of the curve). Step kinematics, toe clearance and knee and hip flexion/extension, hip ab/adduction for one stride of each limb were generated from video of 13 males running the curve during an international 200 m transtibial-classified competition...
March 21, 2017: Journal of Sports Sciences
https://www.readbyqxmd.com/read/28320908/reaction-and-relaxation-at-surface-hotspots-using-molecular-dynamics-and-the-energy-grained-master-equation-to-describe-diamond-etching
#19
David R Glowacki, W J Rodgers, Robin Shannon, Struan H Robertson, Jeremy N Harvey
The extent to which vibrational energy transfer dynamics can impact reaction outcomes beyond the gas phase remains an active research question. Molecular dynamics (MD) simulations are the method of choice for investigating such questions; however, they can be extremely expensive, and therefore it is worth developing cheaper models that are capable of furnishing reasonable results. This paper has two primary aims. First, we investigate the competition between energy relaxation and reaction at 'hotspots' that form on the surface of diamond during the chemical vapour deposition process...
April 28, 2017: Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences
https://www.readbyqxmd.com/read/28320907/a-velocity-map-imaging-study-of-methyl-non-resonant-multiphoton-ionization-from-the-photodissociation-of-ch3i-in-the-a-band
#20
Sonia Marggi Poullain, David V Chicharro, Luis Rubio-Lago, Alberto García-Vela, Luis Bañares
Chemical reaction dynamics and, particularly, photodissociation in the gas phase are generally studied using pump-probe schemes where a first laser pulse induces the process under study and a second one detects the produced fragments. Providing an efficient detection of ro-vibrationally state-selected photofragments, the resonance enhanced multiphoton ionization (REMPI) technique is, without question, the most popular approach used for the probe step, while non-resonant multiphoton ionization (NRMPI) detection of the products is scarce...
April 28, 2017: Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences
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