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https://www.readbyqxmd.com/read/28644752/expression-and-purification-of-swine-rag2-in-e-coli-for-production-of-porcine-rag2-polyclonal-antibodies
#1
Yu-Bei Jin, Wen-Tao Yang, Ke-Yan Huang, Hong-Liang Chen, Seria-Masole Shonyela, Jing Liu, Qiong Liu, Bo Feng, You Zhou, Shu-Li Zhi, Yan-Long Jiang, Jian-Zhong Wang, Hai-Bin Huang, Chun-Wei Shi, Gui-Lian Yang, Chun-Feng Wang
Recombination activating gene 2 (RAG2) is necessary for immature B cell differentiation. Antibodies to human and rabbit RAG2 are currently commercially available, but antibodies to swine RAG remain unavailable to date. In this study, the swine RAG2 genes sequence was synthesized and then cloned into a pET-28a vector. The recombinant fusion protein was successfully expressed in E. coli, purified through nickel column chromatography, and further digested with Tobacco Etch Virus protease. The cleaved protein was purified by molecular-exclusion chromatography and named pRAG2...
June 23, 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/28643180/first-complete-genome-sequences-of-genogroup-v-genotype-3-porcine-sapoviruses-common-5-terminal-genomic-feature-of-sapoviruses
#2
Tomoichiro Oka, Yen Hai Doan, Takashi Shimoike, Kei Haga, Takenori Takizawa
Sapoviruses (SaVs) are enteric viruses and have been detected in various mammals. They are divided into multiple genogroups and genotypes based on the entire major capsid protein (VP1) encoding region sequences. In this study, we determined the first complete genome sequences of two genogroup V, genotype 3 (GV.3) SaV strains detected from swine fecal samples, in combination with Illumina MiSeq sequencing of the libraries prepared from viral RNA and PCR products. The lengths of the viral genome (7494 nucleotides [nt] excluding polyA tail) and short 5'-untranslated region (14 nt) as well as two predicted open reading frames are similar to those of other SaVs...
June 22, 2017: Virus Genes
https://www.readbyqxmd.com/read/28639938/a-pilot-study-of-large-scale-production-of-mutant-pigs-by-enu-mutagenesis
#3
Tang Hai, Chunwei Cao, Haitao Shang, Weiwei Guo, Yanshuang Mu, Shulin Yang, Ying Zhang, Qiantao Zheng, Tao Zhang, Xianlong Wang, Yu Liu, Qingran Kong, Kui Li, Dayu Wang, Meng Qi, Qianlong Hong, Rui Zhang, Xiupeng Wang, Qitao Jia, Xiao Wang, Guosong Qin, Yongshun Li, Ailing Luo, Weiwu Jin, Jing Yao, Jiaojiao Huang, Hongyong Zhang, Menghua Li, Xiangmo Xie, Xuejuan Zheng, Kenan Guo, Qinhua Wang, Shibin Zhang, Liang Li, Fei Xie, Yu Zhang, Xiaogang Weng, Zhi Yin, Kui Hu, Yimei Cong, Peng Zheng, Hailong Zou, Leilei Xin, Jihan Xia, Jinxue Ruan, Hegang Li, Weiming Zhao, Jing Yuan, Zizhan Liu, Weiwang Gu, Ming Li, Yong Wang, Hongmei Wang, Shiming Yang, Zhonghua Liu, Hong Wei, Jianguo Zhao, Qi Zhou, Anming Meng
N-ethyl-N-nitrosourea (ENU) mutagenesis is a powerful tool to efficiently generate large scale of mutants and discover genes with novel functions at the whole-genome level in C. elegans, flies, zebrafish and mice, but has never been tried in large model animals. In the current study, we reported a successful systematic three-generation ENU mutagenesis screening in pigs with the establishment of Chinese Swine Mutagenesis Consortium. A total of 6,770 G1 and 6,800 G3 pigs were screened, 36 dominant and 91 recessive novel pig families with various phenotypes were established...
June 22, 2017: ELife
https://www.readbyqxmd.com/read/28631596/cholesterol-25-hydroxylase-acts-as-a-host-restriction-factor-on-pseudorabies-virus-replication
#4
Jiang Wang, Lei Zeng, Li Zhang, Zhen-Zhen Guo, Shao-Fang Lu, Sheng-Li Ming, Guo-Li Li, Bo Wan, Ke-Gong Tian, Guo-Yu Yang, Bei-Bei Chu
Cholesterol 25-hydroxylase (CH25H) catalyses the production of 25-hydroxycholesterol (25HC) from cholesterol by adding a second hydroxyl group at position 25. The aim of this study was to examine the antiviral effect of CH25H on pseudorabies virus (PRV), a swine pathogen that can cause devastating disease and economic losses worldwide. The results showed that porcine ch25h was induced by either interferon or PRV infection. PRV infection of porcine alveolar macrophages (3D4/21 cells) was attenuated by CH25H overexpression and enhanced by silencing of CH25H...
June 20, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28629420/comparison-of-the-effects-of-platelet-rich-or-growth-factor-rich-plasma-on-intestinal-anastomosis-healing-in-pigs
#5
Gessica Giusto, Cristina Vercelli, Selina Iussich, Massimiliano Tursi, Giovanni Perona, Marco Gandini
BACKGROUND: The use of autologous platelet-rich plasma (PRP) and plasma rich in growth factors (PRGF) has been proposed for the treatment of several acute and chronic syndromes, such as corneal epithelial defects and dry eye syndrome, gum bleeding during oral surgery, and in orthopaedic surgery. We hypothesized that PRGF, rather than PRP, could be more effective because of its intrinsic characteristics in promoting the healing of intestinal anastomosis. The purpose of the present study was to evaluate and compare the effects of PRP and PRGF on various parameters of anastomotic healing in a swine model...
June 19, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28627373/development-and-application-of-a-population-physiologically-based-pharmacokinetic-model-for-penicillin-g-in-swine-and-cattle-for-food-safety-assessment
#6
Miao Li, Ronette Gehring, Jim E Riviere, Zhoumeng Lin
Penicillin G is a widely used antimicrobial in food-producing animals, and one of the most predominant drug residues in animal-derived food products. Due to reduced sensitivity of bacteria to penicillin, extralabel use of penicillin G is common, which may lead to violative residues in edible tissues and cause adverse reactions in consumers. This study aimed to develop a physiologically based pharmacokinetic (PBPK) model to predict drug residues in edible tissues and estimate extended withdrawal intervals for penicillin G in swine and cattle...
June 13, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28627067/a-by-product-of-swine-slaughtering-as-a-protein-source-in-broiler-diets-effects-on-performance-composition-of-excreta-litter-quality-and-on-foot-pad-health
#7
M Kölln, A Loi-Brügger, J Kamphues
Foot pad dermatitis (FPD) is of great concern in poultry industry, and dietary strategies are needed to improve foot pad health because of animal welfare and economic reasons. As the main factor for the development of FPD is the DM content of litter (consisting mainly of excreta; Kamphues et al., 2011), there are different dietary approaches to influence this disease pattern. In two consecutive trials, a total of 200 broilers were kept from day 7 until the 35th day of life. They were divided into four groups at each trial and fed with one of four experimental diets, based on wheat and corn mainly, but differing in the protein source: Group 1 was fed a diet with soya bean meal (SBM) as the main protein source, whereas Group 2, Group 3 and Group 4 were assigned to diets with 4, 8 and 12% of a protein-rich (66...
June 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/28624926/limited-awareness-of-animal-influenza-prevention-and-control-among-dai-lue-smallholder-farmers-in-southwest-china
#8
Xiang Zhao, Gareth Davey
Awareness of animal influenza and its prevention and control is important for ensuring livestock health, production and welfare. In China, a country stereotyped as a major source of emerging zoonotic infectious diseases, research on the public understanding of animal influenza is limited to the Han, the main ethnic group. The present qualitative study in Southwest China investigated awareness of animal influenza among the Dai, an ethnic minority. The participants (15 men and 10 women, ages 18-83) were smallholder farmers of pigs and poultry in rural areas of Jinghong, Xishuangbanna, Yunnan Province...
June 17, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28622866/pathology-and-genetic-findings-in-a-rare-case-of-mycobacterium-caprae-infection-in-a-sow
#9
Benedetta Amato, Teresa Maria Capucchio, Elena Biasibetti, Elena Mangano, Beatrice Maria Boniotti, Lodovica Maria Pacciarini, Sergio Migliore, Maria Vitale, Michele Fiasconaro, Vincenzo Di Marco Lo Presti
Bovine tuberculosis, a reemerging zoonosis in diverse ecological scenarios, has been reported in the autochthonous Nebrodi black pig breed population used for meat production in Italy. During a routine abattoir inspection in 2013, 24 of 299 carcasses (8%) of Nebrodi black pigs presented tuberculosis-like lesions at pathologic examination. Mycobacterium bovis was isolated from 23 animals and M. caprae from a 3-year-old sow. The sow showed severe diffuse lesions involving the visceral organs, right coxofemoral joint, and mammary glands...
June 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28622863/down-regulating-heat-shock-protein-27-is-involved-in-porcine-epidemic-diarrhea-virus-escaping-from-host-antiviral-mechanism
#10
Min Sun, Zeyanqiu Yu, Jiale Ma, Zihao Pan, Chengping Lu, Huochun Yao
Porcine epidemic diarrhea virus (PEDV), is the etiological agent of porcine epidemic diarrhea virus (PED), causes high mortality with severe vomiting, diarrhea, and dehydration in swine farms. In this study, the PEDV strain 85-7 could be proliferated effectively in MARC-145 cells, and caused a distinct inhibition of the expression of interferon-β (IFN-β, encoded by IFNB1), which suggested that a full understanding how this virus manipulates the host immune responses is critical in the fight against the spread of PEDV...
June 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28619166/surveillance-of-antimicrobial-resistance-among-escherichia-coli-from-chicken-and-swine-china-2008-2015
#11
Peng Zhang, Zhangqi Shen, Chunping Zhang, Li Song, Bing Wang, Jun Shang, Xiuying Yue, Zhina Qu, Xinnan Li, Liqin Wu, Yongjun Zheng, Anand Aditya, Yang Wang, Shixin Xu, Congming Wu
The objective of this study was to investigate the antimicrobial resistance trend in Escherichia coli from food animals in China. During 2008-2015, a total of 15,130 E. coli were isolated from chicken and swine from seven provinces. The susceptibilities of these isolates to nine classes of antimicrobial agents were determined using broth microdilution susceptibility method. The findings of this study include: (1) multi-drug resistance was highly prevalent in E. coli; (2) these E. coli isolates showed high resistant rate (>80%) to several old drugs, including ampicillin, tetracycline and sulfisoxazole; (3) increasing resistance to colistin, florfenicol and ceftiofur was observed; (4) the E...
May 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28608607/zearalenone-induces-ros-mediated-mitochondrial-damage-in-porcine-ipec-j2-cells
#12
Wentao Fan, Tongtong Shen, Qiaoqi Ding, Yanan Lv, Li Li, Kehe Huang, Liping Yan, Suquan Song
In this study, the mitochondrial damage effect and mechanism of zearalenone (ZEA) in swine small intestine IPEC-J2 cells in vitro were comprehensively characterized. The analyses revealed that ZEA at high doses (8 and 7 μg/mL) can significantly increase P < 0.05 the malondialdehyde levels and decrease antioxidant enzymes activities after 48 h of exposure. Meanwhile, the reactive oxygen species (ROS) accumulation increased in high dose ZEA-treated groups after 2 h treatment, but decreased due to the ROS-induced mitochondrial damage and the caused cell apoptosis after 48 h of high does ZEA treatment...
June 13, 2017: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/28606148/development-of-a-stochastic-agent-based-model-to-evaluate-surveillance-strategies-for-detection-of-emergent-porcine-reproductive-and-respiratory-syndrome-strains
#13
A G Arruda, Z Poljak, D Knowles, A McLean
BACKGROUND: The objective of the current study was to develop a stochastic agent-based model using empirical data from Ontario (Canada) swine sites in order to evaluate different surveillance strategies for detection of emerging porcine reproductive and respiratory syndrome virus (PRRSV) strains at the regional level. Four strategies were evaluated, including (i) random sampling of fixed numbers of swine sites monthly; (ii) risk-based sampling of fixed numbers, specifically of breeding sites (high-consequence sites); (iii) risk-based sampling of fixed numbers of low biosecurity sites (high-risk); and (iv) risk-based sampling of breeding sites that are characterized as low biosecurity sites (high-risk/high-consequence)...
June 12, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28603717/assessment-of-the-risk-to-public-health-due-to-use-of-antimicrobials-in-pigs-an-example-of-pleuromutilins-in-denmark
#14
Lis Alban, Johanne Ellis-Iversen, Margit Andreasen, Jan Dahl, Ute W Sönksen
Antibiotic consumption in pigs can be optimized by developing treatment guidelines, which encourage veterinarians to use effective drugs with low probability of developing resistance of importance for human health. In Denmark, treatment guidelines for use in swine production are currently under review at the Danish Veterinary and Food Administration. Use of pleuromutilins in swine has previously been associated with a very low risk for human health. However, recent international data and sporadic findings of novel resistance genes suggest a change of risk...
2017: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/28601004/novel-thioglycosyl-analogs-of-glycosyltransferase-substrates-as-antiviral-compounds-against-classical-swine-fever-virus-and-hepatitis-c-virus
#15
Gabriela Pastuch-Gawolek, Binay Chaubey, Boguslaw Szewczyk, Ewelina Krol
Hepatitis C virus (HCV) and classical swine fever virus (CSFV) are important pathogens for which new therapeutic approaches are in high demand. Herein, we report the synthesis of newly designed thioglycosyl analogs of glycosyltransferase substrates which were evaluated using cell-based assays for cytotoxicity and antiviral activity against both viruses. The antiviral activity of synthesized compounds against CSFV and HCV was confirmed using pseudo-plaque reduction assays where a significant arrest of viral growth was observed in the presence of selected compounds...
May 31, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28599000/modeling-the-live-pig-trade-network-in-georgia-implications-for-disease-prevention-and-control
#16
Esther Andrea Kukielka, Beatriz Martínez-López, Daniel Beltrán-Alcrudo
Live pig trade patterns, drivers and characteristics, particularly in backyard predominant systems, remain largely unexplored despite their important contribution to the spread of infectious diseases in the swine industry. A better understanding of the pig trade dynamics can inform the implementation of risk-based and more cost-effective prevention and control programs for swine diseases. In this study, a semi-structured questionnaire elaborated by FAO and implemented to 487 farmers was used to collect data regarding basic characteristics about pig demographics and live-pig trade among villages in the country of Georgia, where very scarce information is available...
2017: PloS One
https://www.readbyqxmd.com/read/28596049/modern-science-for-better-quality-control-of-medicinal-products-towards-global-harmonization-of-3rs-in-biologicals-the-report-of-an-epaa-workshop
#17
Katrin Schutte, Anna Szczepanska, Marlies Halder, Klaus Cussler, Ursula G Sauer, Catrina Stirling, Sylvie Uhlrich, Iwona Wilk-Zasadna, David John, Martin Bopst, Joerg Garbe, Harrie L Glansbeek, Robin Levis, Pieter-Jan Serreyn, Dean Smith, Paul Stickings
This article summarizes the outcome of an international workshop organized by the European Partnership for Alternative Approaches to Animal Testing (EPAA) on Modern science for better quality control of medicinal products: Towards global harmonization of 3Rs in biologicals. As regards the safety testing of biologicals, the workshop participants agreed to actively encourage the deletion of abnormal toxicity tests and target animal batch safety tests from all relevant legal requirements and guidance documents (country-specific guidelines, pharmacopoeia monographs, WHO recommendations)...
June 5, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28592317/modeling-the-spatio-temporal-dynamics-of-porcine-reproductive-respiratory-syndrome-cases-at-farm-level-using-geographical-distance-and-pig-trade-network-matrices
#18
Sara Amirpour Haredasht, Dale Polson, Rodger Main, Kyuyoung Lee, Derald Holtkamp, Beatriz Martínez-López
BACKGROUND: Porcine reproductive and respiratory syndrome (PRRS) is one of the most economically devastating infectious diseases for the swine industry. A better understanding of the disease dynamics and the transmission pathways under diverse epidemiological scenarios is a key for the successful PRRS control and elimination in endemic settings. In this paper we used a two step parameter-driven (PD) Bayesian approach to model the spatio-temporal dynamics of PRRS and predict the PRRS status on farm in subsequent time periods in an endemic setting in the US...
June 7, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28590167/effects-of-in-vivo-and-in-vitro-treatment-of-ascaris-suum-eggs-with-anthelmintic-agents-on-embryonation-and-infectivity-for-mice
#19
Jianguo Zhao, Qian Han, Chenghong Liao, Jinhua Wang, Lili Wu, Qun Liu, David S Lindsay
Ascaris suum is an important intestinal nematode causing economic losses in the swine production industry. Anthelminthic treatment is used to control A. suum infections and is part of normal production practices. Treatment with anthelminthic agents results in expulsion of adult worms from the intestinal tract and ends further contamination of the environment with eggs. The present study was conducted to determine the effects of drug treatment on the embryonation of A. suum eggs collected from worms obtained from pigs treated with 4 different commercially available anthelmintics...
June 7, 2017: Journal of Parasitology
https://www.readbyqxmd.com/read/28589207/prediction-and-in-vitro-verification-of-potential-ctl-epitopes-conserved-among-prrsv-2-strains
#20
Simon Welner, Morten Nielsen, Michael Rasmussen, Søren Buus, Gregers Jungersen, Lars Erik Larsen
Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) is the causative agent of one of the most important porcine diseases with a high impact on animal health, welfare, and production economy. PRRSV exhibits a multitude of immunoevasive strategies that, in combination with a very high mutation rate, has hampered the development of safe and broadly protective vaccines. Aiming at a vaccine inducing an effective cytotoxic T cell response, a bioinformatics approach was taken to identify conserved PRRSV-derived peptides predicted to react broadly with common swine leukocyte antigen (SLA) class I alleles...
June 7, 2017: Immunogenetics
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