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https://www.readbyqxmd.com/read/28107215/metabolic-cardiac-and-renal-effects-of-the-slow-hydrogen-sulfide-releasing-molecule-gyy4137-during-resuscitated-septic-shock-in-swine-with-pre-existing-coronary-artery-disease
#1
Benedikt L Nubaum, Josef Vogt, Ulrich Wachter, Oscar McCook, Martin Wepler, José Matallo, Enrico Calzia, Michael Gröger, Michael Georgieff, Mark E Wood, Matthew Whiteman, Peter Radermacher, Sebastian Hafner
Decreased levels of endogenous hydrogen sulfide (H2S) contribute to atherosclerosis, whereas equivocal data are available on H2S effects during sepsis. Moreover, H2S improved glucose utilization in anaesthetized, ventilated, hypothermic mice, but normothermia and/or sepsis blunted this effect. The metabolic effects of H2S in large animals are controversial. Therefore, we investigated the effects of the H2S donor GYY4137 during resuscitated, fecal peritonitis-induced septic shock in swine with genetically and diet-induced coronary artery disease (CAD)...
January 19, 2017: Shock
https://www.readbyqxmd.com/read/28100261/serotypes-of-streptococcus-suis-isolated-from-healthy-pigs-in-phayao-province-thailand
#2
P Thongkamkoon, T Kiatyingangsulee, M Gottschalk
BACKGROUND: Streptococcus suis (S. suis) is an important swine and human pathogen. There are 33 serotypes that have been described. Zoonotic cases are very common the Northern part of Thailand, especially in Phayao Province. However, the prevalence of S. suis and, more particularly the different serotypes, in pigs in this region is poorly known and needed to be addressed. THE CONTEXT AND PURPOSE OF THE STUDY: Distribution of S. suis serotypes varies depending on the geographical area...
January 19, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28099453/elimination-of-porcine-epidemic-diarrhea-virus-in-an-animal-feed-manufacturing-facility
#3
Anne R Huss, Loni L Schumacher, Roger A Cochrane, Elizabeth Poulsen, Jianfa Bai, Jason C Woodworth, Steve S Dritz, Charles R Stark, Cassandra K Jones
Porcine Epidemic Diarrhea Virus (PEDV) was the first virus of wide scale concern to be linked to possible transmission by livestock feed or ingredients. Measures to exclude pathogens, prevent cross-contamination, and actively reduce the pathogenic load of feed and ingredients are being developed. However, research thus far has focused on the role of chemicals or thermal treatment to reduce the RNA in the actual feedstuffs, and has not addressed potential residual contamination within the manufacturing facility that may lead to continuous contamination of finished feeds...
2017: PloS One
https://www.readbyqxmd.com/read/28098524/swine-influenza-virus-h1n2-characterization-and-transmission-in-ferrets-chile
#4
Nicolás Bravo-Vasquez, Erik A Karlsson, Pedro Jimenez-Bluhm, Victoria Meliopoulos, Bryan Kaplan, Shauna Marvin, Valerie Cortez, Pamela Freiden, Melinda A Beck, Christopher Hamilton-West, Stacey Schultz-Cherry
Phylogenetic analysis of the influenza hemagglutinin gene (HA) has suggested that commercial pigs in Chile harbor unique human seasonal H1-like influenza viruses, but further information, including characterization of these viruses, was unavailable. We isolated influenza virus (H1N2) from a swine in a backyard production farm in Central Chile and demonstrated that the HA gene was identical to that in a previous report. Its HA and neuraminidase genes were most similar to human H1 and N2 viruses from the early 1990s and internal segments were similar to influenza A(H1N1)pdm09 virus...
February 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28095773/dietary-pea-fiber-increases-diversity-of-colonic-methanogens-of-pigs-with-a-shift-from-methanobrevibacter-to-methanomassiliicoccus-like-genus-and-change-in-numbers-of-three-hydrogenotrophs
#5
Yuheng Luo, Hong Chen, Bing Yu, Jun He, Ping Zheng, Xiangbing Mao, Gang Tian, Jie Yu, Zhiqing Huang, Junqiu Luo, Daiwen Chen
BACKGROUND: Pea fiber (PF) is a potential fibrous supplement in swine production. The influence of dietary PF on microbial community in the colon of pigs remains largely unexplored. Methanogens in the hindgut of monogastric animals play important roles in degradation of dietary fibers and efficient removal of microbial metabolic end product H2. Understanding the impact of dietary PF on the structure of colonic methanogens may help understand the mechanisms of microbe-mediated physiological functions of PF...
January 17, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28093083/genetic-analysis-of-teat-number-in-pigs-reveals-some-developmental-pathways-independent-of-vertebra-number-and-several-loci-which-only-affect-a-specific-side
#6
Gary A Rohrer, Dan J Nonneman
BACKGROUND: Number of functional teats is an important trait in commercial swine production. As litter size increases, the number of teats must also increase to supply nutrition to all piglets. Therefore, a genome-wide association analysis was conducted to identify genomic regions that affect this trait in a commercial swine population. Genotypic data from the Illumina Porcine SNP60v1 BeadChip were available for 2951 animals with total teat number (TTN) records. A subset of these animals (n = 1828) had number of teats on each side recorded...
January 4, 2017: Genetics, Selection, Evolution: GSE
https://www.readbyqxmd.com/read/28081386/effects-of-biodiesel-made-from-swine-and-chicken-fat-residues-on-co-co2-and-nox-emissions
#7
Vivian Feddern, Anildo Cunha Junior, Marina C De Prá, Marcio L Busi da Silva, Rodrigo da S Nicoloso, Martha M Higarashi, Arlei Coldebella, Paulo G de Abreu
Implications Emissions from motor vehicles can contribute considerably to the levels of greenhouse gases in the atmosphere. The use of biodiesel to replace or augment diesel can not only decrease our dependency on fossil fuels but also help decrease air pollution. Thus, different sources of feedstocks are constantly being explored for affordable biodiesel production. However, the amount of CO, CO2 and/or NOx emissions can vary largely depending on type of feedstock used to produce biodiesel. In this work we demonstrated animal fat feasibility in replacing petrodiesel with less impact regarding greenhouse gas emissions than other sources...
January 12, 2017: Journal of the Air & Waste Management Association
https://www.readbyqxmd.com/read/28077800/oxidative-stress-induced-by-se-deficient-high-energy-diet-implicates-neutrophil-dysfunction-via-nrf2-pathway-suppression-in-swine
#8
Tianshu Yang, Zeping Zhao, Tianqi Liu, Ziwei Zhang, Pengzu Wang, Shiwen Xu, Xin Gen Len, Anshan Shan
The mechanism of the interaction between Se deficiency and high energy remains limited. The aim of the current study was to identify whether Se-deficient, high-energy diet can induce oxidative stress, and downregulate the Nrf2 pathway and phagocytic dysfunction of neutrophils. We detected the phagocytic activity, ROS production, protein levels of Nrf2 and Nrf2 downstream target genes, and the mRNA levels of 25 selenoproteins, heat shock proteins, and cytokines in neutrophils. Cytokine ELISA kits were used to measure the serum cytokines...
January 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/28069803/ig-light-chain-precedes-heavy-chain-gene-rearrangement-during-development-of-b-cells-in-swine
#9
Marek Sinkora, Jana Sinkorova, Katerina Stepanova
The current mammalian paradigm states that 1) rearrangements in the IgH locus precede those in IgL loci, 2) IgLλ genes rearrange only when IgLκ genes are consumed, and 3) the surrogate L chain is necessary for selection of productive IgH gene rearrangements. We show in swine that IgL rearrangements precede IgH gene rearrangements, resulting in the expression of naked IgL on a surface of precursor B cells. Findings also suggest that there is no dependency on the surrogate L chain, and thus the authentic IgL proteins may be used for selection of the IgH repertoire...
January 9, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28069290/classical-swine-fever-vaccines-state-of-the-art
#10
Sandra Blome, Claudia Moß, Ilona Reimann, Patricia König, Martin Beer
Due to its impact on animal health and pig industry, classical swine fever (CSF) is still one of the most important viral diseases of pigs. To control the disease, safe and highly efficacious live attenuated vaccines exist for decades. These vaccines have usually outstanding efficacy and safety but lack differentiability of infected from vaccinated animals (DIVA or marker strategy). In contrast, the first generation of E2 subunit marker vaccines shows constraints in efficacy, application, and production. To overcome these limitations, new generations of marker vaccines are developed...
January 3, 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28063923/phylogenetic-analysis-of-hepatitis-e-virus-strains-isolated-from-slaughter-age-pigs-in-colombia
#11
Jorge E Forero D, Cristian Gutiérrez-Vergara, Jaime Parra S, Guillermo Correa L, Berardo Rodríguez, Lina A Gutiérrez, Francisco Javier Díaz, Albeiro López-Herrera
Hepatitis E virus (HEV) is a zoonotic virus that causes acute hepatitis in humans, and can be transmitted via the fecal-oral route. Pigs are considered to be a reservoir for this infection-mainly where the disease is not endemic. In a previous study conducted in Antioquia, which is a region in Colombia where the production and consumption of pork meat is higher than in the rest of the country, the presence of anti-HEV IgG-type antibodies was reported in slaughter-age pigs. Aiming to identify the HEV genotype circulating in swine, animal liver, and feces samples from five swine cattle slaughterhouses located in six different sub-regions of Antioquia were collected...
January 4, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28063479/positive-immunomodulatory-effects-of-heterologous-dna-vaccine-modified-live-vaccine-prime-boost-immunization-against-the-highly-pathogenic-prrsv-infection
#12
Chaitawat Sirisereewan, Teerawut Nedumpun, Sawang Kesdangsakonwut, Yonlayong Woonwong, Roongtham Kedkovid, Jirapat Arunorat, Roongroje Thanawongnuwech, Sanipa Suradhat
Porcine reproductive and respiratory syndrome virus (PRRSV) infection is one of the most important swine pathogens, and causes a major economic impact worldwide. Recently, a new variant type 2 PRRSV, highly pathogenic PRRSV (HP-PRRSV) has emerged and continued to circulate in Southeast Asia region. Currently, commercially available PRRSV vaccines, modified live PRRS vaccines (MLV) are not able to provide complete protection against HP-PRRSV and been reported to induce negative immunomodulatory effects. Interestingly, a novel DNA vaccine was developed and successfully used to improve PRRSV-specific immune responses following MLV vaccination...
January 2017: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/28061998/effects-of-mycoplasma-hyopneumoniae-on-porcine-nasal-cavity-dendritic-cells
#13
Yumeng Shen, Weiwei Hu, Yanna Wei, Zhixin Feng, Qian Yang
Mycoplasma hyopneumoniae (Mhp) is the primary etiological agent responsible for swine enzootic pneumonia (EP), a disease that cause tremendous economic losses all over the swine industry. Dendritic cells (DCs), the most effective antigen-presenting cells, are widely distributed beneath respiratory epithelium. DCs uptake and present antigens to T cells, to initiate protective immune responses or generate immune-mediated pathology in different infections. In this study, we investigated the changes in the different DCs subpopulations, T cells and SIgA positive cells counts in porcine nasal cavity after long time Mhp infection...
January 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28061786/attenuated-actinobacillus-pleuropneumoniae-double-deletion-mutant-s-8%C3%A2-clpp-apxiic-confers-protection-against-homologous-or-heterologous-strain-challenge
#14
Fang Xie, Gang Li, Long Zhou, Yanhe Zhang, Ning Cui, Siguo Liu, Chunlai Wang
BACKGROUND: Actinobacillus pleuropneumoniae is the etiological agent of porcine pleuropneumonia, which leads to large economic losses to the swine industry worldwide. In this study, S-8△clpP△apxIIC, a double-deletion mutant of A. pleuropneumoniae was constructed, and its safety and protective efficacy were evaluated in pigs. RESULTS: The S-8△clpP△apxIIC mutant exhibited attenuated virulence in a murine (BALB/c) model, and caused no detrimental effects on pigs even at a dose of up to 1...
January 6, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28056965/phylogenetic-characterization-of-genes-encoding-for-viral-envelope-glycoprotein-orf5-and-nucleocapsid-protein-orf7-of-porcine-reproductive-respiratory-syndrome-virus-found-in-malaysia-in-2013-and-2014
#15
Seetha Jaganathan King, Peck Toung Ooi, Lai Yee Phang, Zeenathul Nazariah Binti Allaudin, Wei Hoong Loh, Chiou Yan Tee, Shiao Pau How, Lai Siong Yip, Pow Yoon Choo, Ban Keong Lim
BACKGROUND: Porcine reproductive and respiratory syndrome (PRRS) is one of the most expensive diseases of modern swine production & results in annual economic losses and cost the industry over 600 million USD in U.S. alone and billions of dollars worldwide. Two atypical PRRS cases were observed in 2013 and 2014 characterized by late-term abortion, fever and sudden increase in sow mortality which persisted for a prolonged period of time. METHODS: Lungs, lymph nodes and other samples were collected for disease investigation...
January 5, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28056961/rope-based-oral-fluid-sampling-for-early-detection-of-classical-swine-fever-in-domestic-pigs-at-group-level
#16
Klaas Dietze, Anna Tucakov, Tatjana Engel, Sabine Wirtz, Klaus Depner, Anja Globig, Robert Kammerer, Susan Mouchantat
BACKGROUND: Non-invasive sampling techniques based on the analysis of oral fluid specimen have gained substantial importance in the field of swine herd management. Methodological advances have a focus on endemic viral diseases in commercial pig production. More recently, these approaches have been adapted to non-invasive sampling of wild boar for transboundary animal disease detection for which these effective population level sampling methods have not been available. In this study, a rope-in-a-bait based oral fluid sampling technique was tested to detect classical swine fever virus nucleic acid shedding from experimentally infected domestic pigs...
January 5, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28056374/kinetic-parameter-estimation-model-for-anaerobic-co-digestion-of-waste-activated-sludge-and-microalgae
#17
Eunyoung Lee, Jewel Cumberbatch, Meng Wang, Qiong Zhang
Anaerobic co-digestion has a potential to improve biogas production, but limited kinetic information is available for co-digestion. This study introduced regression-based models to estimate the kinetic parameters for the co-digestion of microalgae and Waste Activated Sludge (WAS). The models were developed using the ratios of co-substrates and the kinetic parameters for the single substrate as indicators. The models were applied to the modified first-order kinetics and Monod model to determine the rate of hydrolysis and methanogenesis for the co-digestion...
December 23, 2016: Bioresource Technology
https://www.readbyqxmd.com/read/28051323/bisphenol-a-interferes-with-swine-vascular-endothelial-cell-functions
#18
Giuseppina Basini, Simona Bussolati, Stefano Grolli, Roberto Ramoni, Francesca Grasselli
Several studies have demonstrated that the endocrine disruptor bisphenol A (BPA) negatively affects animal and human health. An angiogenic process has been suggested among the events disrupted by this molecule, but the underlying mechanisms have not yet been clarified. The effect of BPA on angiogenesis was investigated by means of a bioassay previously validated in our laboratory. Using immortalized swine aortic endothelial cell line (AOC), the development of new blood vessels through a three-dimensional in vitro angiogenesis assay was evaluated...
October 20, 2016: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/28050660/new-alcohol-and-onyx-mixture-for-embolization-feasibility-and-proof-of-concept-in-both-in-vitro-and-in-vivo-models
#19
Mohammad Saeed Kilani, Fatemeh Zehtabi, Sophie Lerouge, Gilles Soulez, Jean Michel Bartoli, Vincent Vidal, Mohammad F Badran
INTRODUCTION: Onyx and ethanol are well-known embolic and sclerotic agents that are frequently used in embolization. These agents present advantages and disadvantages regarding visibility, injection control and penetration depth. Mixing both products might yield a new product with different characteristics. The aim of this study is to evaluate the injectability, radiopacity, and mechanical and occlusive properties of different mixtures of Onyx 18 and ethanol in vitro and in vivo (in a swine model)...
January 3, 2017: Cardiovascular and Interventional Radiology
https://www.readbyqxmd.com/read/28044192/porcine-mast-cells-infected-with-h1n1-influenza-virus-release-histamine-and-inflammatory-cytokines-and-chemokines
#20
In Hong Lee, Hyun Soo Kim, Sang Heui Seo
Mast cells reside in many tissues, including the lungs, and might play a role in enhancing influenza virus infections in animals. In this study, we cultured porcine mast cells from porcine bone marrow cells with IL-3 and stem cell factor to study the infectivity and activation of the 2009 pandemic H1N1 influenza virus of swine origin. Porcine mast cells were infected with H1N1 influenza virus, without the subsequent production of infectious viruses but were activated, as indicated by the release of histamines...
January 2, 2017: Archives of Virology
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