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https://www.readbyqxmd.com/read/29118429/mesenchymal-stem-cells-internalize-mycobacterium-tuberculosis-through-scavenger-receptors-and-restrict-bacterial-growth-through-autophagy
#1
Arshad Khan, Lovepreet Mann, Ramesha Papanna, Mi-Ae Lyu, Christopher R Singh, Scott Olson, N Tony Eissa, Jeffrey Cirillo, Gobardhan Das, Robert L Hunter, Chinnaswamy Jagannath
Human mesenchymal stem cells (MSCs) express scavenger receptors that internalize lipids, including oxidized low-density lipoprotein (oxLDL). We report that MSCs phagocytose Mycobacterium tuberculosis (Mtb) through two types of scavenger receptors (SRs; MARCO and SR-B1), as blockade of the receptors with antibodies or siRNA knockdown decreased the uptake of Mtb. MSCs also expressed mannose receptor (MR) that was found to endocytose rhodamine-labeled mannosylated BSA (rMBSA), though the receptor was not involved in the uptake of Mtb...
November 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28983891/infection-and-failure-rates-following-total-hip-arthroplasty-for-septic-arthritis-a-case-controlled-study
#2
Madhavan C Papanna, Ryad Chebbout, Simon Buckley, Ian Stockley, Andrew Hamer
INTRODUCTION: Total hip arthroplasty (THA) is performed as a 2-stage or single stage procedure in patients with a history of septic arthritis of the native hip joint. The decision is based on whether the infection is active or quiescent in the joint. The aim of this study was to compare the outcomes of total hip arthroplasty for septic arthritis of the native hip to a control series of patients treated for primary osteoarthritis of the hip with standard THA. METHODS: Between March 2000 and March 2013, 18 cases of septic arthritis of the hip treated with THA were retrospectively identified...
October 4, 2017: Hip International: the Journal of Clinical and Experimental Research on Hip Pathology and Therapy
https://www.readbyqxmd.com/read/28925589/recurrent-twin-twin-transfusion-syndrome-rttts-and-twin-anemia-polycythemia-sequence-taps-after-fetoscopic-laser-surgery-fls-size-of-the-cannula-does-matter
#3
Roopali Donepudi, Joost Akkermans, Lovepreet Mann, Frans J Klumper, Johanna M Middeldorp, Enrico Lopriore, Kenneth J Moise, Michael Bebbington, Anthony Johnson, Dick Oepkes, Ramesha Papanna
OBJECTIVES: Optimal outcomes after fetoscopic laser surgery (FLS) for twin-twin transfusion (TTTS) depend on the successful completion of ablation of all placental anastomoses. Our objective was to determine the incidence and risk factors for the occurrence of recurrent TTTS (rTTTS) or twin anemia-polycythemia sequence (TAPS) after FLS, and focusing on the impact of different diameters of the cannula. METHODS: A secondary analysis of prospectively collected data of 666 consecutive patients undergoing laser surgery for TTTS from 2 centers was performed...
September 19, 2017: Ultrasound in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/28828062/femoral-neck-fractures-in-osteogenesis-imperfecta-treated-with-bisphosphonates
#4
M C Papanna, S Tafazal, M J Bell, S N Giles, J A Fernandes
PURPOSE: Osteogenesis imperfecta (OI) is a condition characterised by bone fragility and multiple fractures, which cause considerable morbidity in the affected patients. Most cases are associated with mutations in one of the type I collagen genes. Recently, bisphosponates have been used widely to reduce pain and the incidence of fragility fractures in OI in children, even though there have been concerns raised regarding the long-term complications of it due to their effect on the bone...
June 1, 2017: Journal of Children's Orthopaedics
https://www.readbyqxmd.com/read/28697490/the-influence-of-blood-pressure-on-fetal-aortic-distensibility-an-animal-validation-study
#5
Christoph Wohlmuth, Kenneth J Moise, Ramesha Papanna, Ciprian Gheorghe, Anthony Johnson, Yisel Morales, Helena M Gardiner
BACKGROUND/AIMS: Aortic distension waveforms describe the change in diameter or cross-sectional area over the cardiac cycle. We aimed to validate the association of aortic fractional area change (AFAC) with blood pressure (BP) in a fetal lamb model. METHODS: Four pregnant ewes underwent open fetal surgery under general anesthesia at 107-120 gestational days. A 4-Fr catheter was introduced into the fetal femoral artery and vein, or the carotid artery and jugular vein...
July 12, 2017: Fetal Diagnosis and Therapy
https://www.readbyqxmd.com/read/28455084/recipient-umbilical-artery-elongation-redundancy-in-twin-twin-transfusion-syndrome
#6
COMPARATIVE STUDY
Roopali Donepudi, Lovepreet K Mann, Christoph Wohlmuth, Anthony Johnson, Michael W Bebbington, Kenneth J Moise, David S Boudreaux, Helena Gardiner, Ramesha Papanna
BACKGROUND: Chronic hypertension in adults causes arterial lengthening in major arteries, but the effects of early fetal hypertension on the twin-twin transfusion syndrome recipient's vascular architecture remains unknown. OBJECTIVE: We hypothesize that arterial cord redundancy is related to recipient hypertension and subsequent heart failure. Our objectives were to: (1) establish a 3-dimensional color Doppler ultrasound method of measuring umbilical arterial length relative to its corresponding venous segment in the umbilical cord using artery vein angle; (2) compare recipient artery vein angle to gestational age-matched controls; and (3) test the association of artery vein angle with recipient heart failure...
August 2017: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/28400306/can-3-dimensional-power-doppler-indices-improve-the-prenatal-diagnosis%C3%A2-of-a-potentially-morbidly-adherent-placenta-in-patients-with%C3%A2-placenta-previa
#7
Ziad A Haidar, Ramesha Papanna, Baha M Sibai, Nina Tatevian, Oscar A Viteri, Patricia C Vowels, Sean C Blackwell, Kenneth J Moise
BACKGROUND: Traditionally, 2-dimensional ultrasound parameters have been used for the diagnosis of a suspected morbidly adherent placenta previa. More objective techniques have not been well studied yet. OBJECTIVE: The objective of the study was to determine the ability of prenatal 3-dimensional power Doppler analysis of flow and vascular indices to predict the morbidly adherent placenta objectively. STUDY DESIGN: A prospective cohort study was performed in women between 28 and 32 gestational weeks with known placenta previa...
August 2017: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/28370497/cardiac-pathophysiology-in-twin-twin-transfusion-syndrome-new-insights-into-its-evolution
#8
Christoph Wohlmuth, David Boudreaux, Kenneth J Moise, Anthony Johnson, Ramesha Papanna, Michael Bebbington, Helena M Gardiner
OBJECTIVES: In twin-twin transfusion syndrome (TTTS) unbalanced transfer of vasoactive mediators and fluid from the 'donor' to the 'recipient' alters cardiovascular function. We evaluated cardiac function in 145 monochorionic-diamniotic (MCDA) pregnancies with and without TTTS. METHODS: Echocardiography was performed in uncomplicated MCDA, n = 26; TTTS stage I+II, n = 61; TTTS stage III+IV, n = 58, before selective fetoscopic laser photocoagulation (SFLP) in TTTS, and afterwards in 41 (34%) at a mean of 1...
March 31, 2017: Ultrasound in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/28295455/cryopreserved-human-umbilical-cord-versus-biocellulose-film-for-prenatal-spina-bifida-repair-in-a-physiologic-rat-model
#9
Saul Snowise, Lovepreet Mann, Yisel Morales, Kenneth J Moise, Anthony Johnson, Stephen Fletcher, Raymond J Grill, Scheffer C G Tseng, Ramesha Papanna
BACKGROUND: Prenatal spina bifida (SB) repair with a regenerative patch may improve neurological outcomes by decreasing inflammatory scarring. OBJECTIVE: This study aims to compare cryopreserved human umbilical cord (HUC) and biocellulose film (BCF) patches sutured over SB lesions for regeneration of native cells and inflammatory response. STUDY DESIGN: Sprague-Dawley rats were gavaged with retinoic acid (RA) on embryonic day 10 to induce SB...
March 13, 2017: Prenatal Diagnosis
https://www.readbyqxmd.com/read/28221114/mycobacterium-tuberculosis-infection-in-free-roaming-wild-asian-elephant
#10
Basavegowdanadoddi Marinaik Chandranaik, Beechagondahalli Papanna Shivashankar, Kunigal Srinivasa Umashankar, Poojappa Nandini, Papanna Giridhar, Somenahalli Munivenkatappa Byregowda, Basavegowdanadoddi Marinaik Shrinivasa
Postmortem examination of a wild Asian elephant at Rajiv Gandhi National Park, India, revealed nodular lesions, granulomas with central caseation, and acid-fast bacilli in the lungs. PCR and nucleotide sequencing confirmed the presence of Mycobacterium tuberculosis. This study indicates that wild elephants can harbor M. tuberculosis that can become fatal.
March 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28153514/association-of-acute-toxic-encephalopathy-with-litchi-consumption-in-an-outbreak-in-muzaffarpur-india-2014-a-case-control-study
#11
Aakash Shrivastava, Anil Kumar, Jerry D Thomas, Kayla F Laserson, Gyan Bhushan, Melissa D Carter, Mala Chhabra, Veena Mittal, Shashi Khare, James J Sejvar, Mayank Dwivedi, Samantha L Isenberg, Rudolph Johnson, James L Pirkle, Jon D Sharer, Patricia L Hall, Rajesh Yadav, Anoop Velayudhan, Mohan Papanna, Pankaj Singh, D Somashekar, Arghya Pradhan, Kapil Goel, Rajesh Pandey, Mohan Kumar, Satish Kumar, Amit Chakrabarti, P Sivaperumal, A Ramesh Kumar, Joshua G Schier, Arthur Chang, Leigh Ann Graham, Thomas P Mathews, Darryl Johnson, Liza Valentin, Kathleen L Caldwell, Jeffery M Jarrett, Leslie A Harden, Gary R Takeoka, Suxiang Tong, Krista Queen, Clinton Paden, Anne Whitney, Dana L Haberling, Ram Singh, Ravi Shankar Singh, Kenneth C Earhart, A C Dhariwal, L S Chauhan, S Venkatesh, Padmini Srikantiah
BACKGROUND: Outbreaks of unexplained illness frequently remain under-investigated. In India, outbreaks of an acute neurological illness with high mortality among children occur annually in Muzaffarpur, the country's largest litchi cultivation region. In 2014, we aimed to investigate the cause and risk factors for this illness. METHODS: In this hospital-based surveillance and nested age-matched case-control study, we did laboratory investigations to assess potential infectious and non-infectious causes of this acute neurological illness...
April 2017: Lancet Global Health
https://www.readbyqxmd.com/read/27644453/abstracts-of-the-26th-world-congress-on-ultrasound-in-obstetrics-and-gynecology-rome-italy-24-28-september-2016
#12
C Wohlmuth, F A Osei, K J Moise, A Johnson, R Papanna, M Bebbington, H Gardiner
No abstract text is available yet for this article.
September 2016: Ultrasound in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/27621952/neurological-outcomes-after-human-umbilical-cord-patch-for-in-utero-spina-bifida-repair-in-a-sheep-model
#13
Ramesha Papanna, Lovepreet K Mann, Saul Snowise, Yisel Morales, Sanjay P Prabhu, Scheffer C G Tseng, Raymond Grill, Stephen Fletcher, Kenneth J Moise
OBJECTIVES: The objective of our study was to test the hypothesis that in utero repair of surgically created spina bifida in a sheep model using cryopreserved human umbilical cord (HUC) patch improves neurological outcome. METHODS: Spina bifida with myelotomy was surgically created in timed pregnant ewes at gestational day (GD) 75. The fetuses were randomly assigned to unrepaired versus HUC and treated at GD 95 and then delivered at GD 140. Neurological evaluation was performed using the Texas Spinal Cord Injury Scale (TSCIS), bladder control using ultrasound, and the hindbrain herniation...
July 2016: American Journal of Perinatology Reports
https://www.readbyqxmd.com/read/27407191/a-gradient-based-facile-hplc-method-for-simultaneous-estimation-of-antioxidants-extracted-from-tea-powder
#14
Shankara H Nanjegowda, Manasa G Papanna, Raghu Ram Achar, Kanchugarakoppal S Rangappa, Puttaswamappa Mallu, Shivananju Nanjunda Swamy
A new simple, rapid and precise RP-HPLC method was developed for the extraction and quantitative estimation of caffeine (C), (-)-epigallocatechin gallate (EGCG), (+)-catechin(Ct), (-)-epicatechin(EC), and (-)-epicatechin gallate (ECG) (collectively named as Tea Powder Bioactives TPBAs) extracted from tea powder using different ratios of ethanol: water. The simultaneous determination of TPBAs was performed using the UV spectrophotometric method which employs the absorbance at 205 nm (λmax of caffeine and polyphenols)...
May 2016: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/27400004/cryopreserved-human-umbilical-cord-for-in-utero-myeloschisis-repair
#15
Ramesha Papanna, Stephen Fletcher, Kenneth J Moise, Lovepreet K Mann, Scheffer C G Tseng
BACKGROUND: There is an urgent need is for a regenerative patch for in utero repair of spina bifida in situations in which primary closure is difficult. CASES: We present two cases of large myeloschisis defects that underwent in utero spina bifida repair at midgestation with closure of the skin defect by using a cryopreserved human umbilical cord patch. The pregnancies were uncomplicated, and the births occurred at 37 weeks by planned cesarean delivery. The repair sites were intact with no evidence of cerebrospinal fluid leakage, with skin regenerated after delivery over a period of 3-4 weeks...
August 2016: Obstetrics and Gynecology
https://www.readbyqxmd.com/read/27222097/preterm-delivery-after-fetoscopic-laser-surgery-for-twin-twin-transfusion-syndrome-etiology-and-risk-factors
#16
A Malshe, S Snowise, L K Mann, N Boring, A Johnson, M W Bebbington, K J Moise, R Papanna
OBJECTIVE: Preterm delivery after fetoscopic laser surgery (FLS) for twin-twin transfusion syndrome (TTTS) is a major complication. The causative factors leading to preterm delivery continue to be elusive and a better understanding of the risk factors could reduce complications. The objective of this study was to determine the etiology of preterm delivery after FLS for TTTS and its associated risk factors. METHODS: This was a secondary analysis of a prospective study of 203 patients with TTTS who underwent FLS at a single center between September 2011 and December 2014...
May 2017: Ultrasound in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/27153404/preterm-prelabor-rupture-of-membranes-after-fetoscopic-laser-surgery-for-twin-twin-transfusion-syndrome
#17
S Snowise, L K Mann, K J Moise, A Johnson, M W Bebbington, R Papanna
OBJECTIVE: To assess the incidence, clinical course, risk factors and outcomes of preterm prelabor rupture of membranes (PPROM) after fetoscopic laser surgery (FLS) for twin-twin transfusion syndrome (TTTS). METHODS: This was a prospective observational cohort study of 154 consecutive cases of TTTS. PPROM was defined as rupture of membranes before 34 weeks' gestation. Procedure-to-PPROM and PPROM-to-delivery intervals were determined. Relevant preoperative and intraoperative variables were analyzed by univariate and multivariate logistic regression to determine their impact on PPROM after FLS...
May 2017: Ultrasound in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/26989864/changes-in-ductus-venosus-flow-profile-in-twin-twin-transfusion-syndrome-role-in-risk-stratification
#18
C Wohlmuth, F A Osei, K J Moise, I Wieser, A Johnson, R Papanna, M Bebbington, H M Gardiner
OBJECTIVES: To evaluate changes in ductus venosus (DV) waveforms and the timing of these changes in twin-twin transfusion syndrome (TTTS), to relate these to disease severity and to assess the clinical applicability of the suggested measurements in the prediction of TTTS. METHODS: DV time intervals and velocity-time integrals (VTI) normalized to cardiac cycle and total VTI, respectively, as well as velocity ratios were analyzed in 149 monochorionic diamniotic (MCDA) twin pairs...
December 2016: Ultrasound in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/26984411/the-use-of-recombinant-morphogenic-protein-2-rhbmp-2-in-children-undergoing-revision-surgery-for-persistent-non-union
#19
Madhavan C Papanna, K A Saldanha, Binu Kurian, James A Fernandes, Stan Jones
The purpose of the study was to evaluate the safety and efficacy with the use of BMP-2 for treating persistent non-unions in children with underlying complex conditions. Between October 2006 and November 2010 in our unit, 15 patients were treated with rhBMP-2 to enhance bone union. There were nine females and six males with a mean age of 9.5 years (range 4-15) at time of surgery. Seventy-five per cent of the patients required revision of internal fixation with insertion of rhBMP-2 to the non-union site, and the reminder had freshening of the non-union site with rhBMP-2 application...
April 2016: Strategies in Trauma and Limb Reconstruction
https://www.readbyqxmd.com/read/26663469/aortic-distensibility-as-a-surrogate-for-intertwin-pulse-pressure-differences-in-monochorionic-pregnancies-with-and%C3%A2-without-twin-twin-transfusion-syndrome
#20
C Wohlmuth, F A Osei, K J Moise, A Johnson, R Papanna, M Bebbington, H M Gardiner
OBJECTIVES: Twin-twin transfusion syndrome (TTTS) complicates 10-15% of monochorionic diamniotic (MCDA) twin pregnancies. The donor response to hypovolemia allows the transfer of vasoactive mediators to the recipient, causing increased recipient afterload and hypertension. Our objective was to apply a novel speckle-tracking technique to measure the aortic fractional area change (AFAC) during the cardiac cycle in MCDA twins with and without TTTS, and identify intertwin differences in AFAC and parameters of cardiac function...
August 2016: Ultrasound in Obstetrics & Gynecology
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